mRNA_S-ischiensis_contig91.20923.1 (mRNA) Schizocladia ischiensis KU_0333

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-ischiensis_contig91.20923.1
Unique NamemRNA_S-ischiensis_contig91.20923.1
TypemRNA
OrganismSchizocladia ischiensis KU_0333 (Schizocladia ischiensis KU_0333)
Homology
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A7M5VAF3_9CNID (Uncharacterized protein n=1 Tax=Clytia hemisphaerica TaxID=252671 RepID=A0A7M5VAF3_9CNID)

HSP 1 Score: 63.9 bits (154), Expect = 1.850e-8
Identity = 26/54 (48.15%), Postives = 38/54 (70.37%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIES 162
            WKF K+ Q WLLQNMYDK+ V +  FK +  YLE L+G  R++ L+ A++ +E+
Sbjct:   98 WKFRKLRQTWLLQNMYDKEKVSKANFKILLEYLEDLKGAAREQTLKEAKEFVEA 151
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A091MK47_9PASS (Uncharacterized protein C7orf50 (Fragment) n=2 Tax=Acanthisitta chloris TaxID=57068 RepID=A0A091MK47_9PASS)

HSP 1 Score: 58.9 bits (141), Expect = 5.880e-7
Identity = 24/53 (45.28%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIE 159
            WKF K  Q WLL +MYDK+ V ++ F  +  YL+GLQGG R + +++A D ++
Sbjct:   68 WKFQKTRQTWLLLHMYDKEKVPDKYFTILLDYLQGLQGGARDKTVQKAEDFMK 120
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: T2M730_HYDVU (Uncharacterized protein C7orf50 n=2 Tax=Hydra vulgaris TaxID=6087 RepID=T2M730_HYDVU)

HSP 1 Score: 57.4 bits (137), Expect = 1.650e-6
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIE 159
            WKF KV Q WLLQNMY+K  V++  FK +  YL  L+G  R++ +E AR ++E
Sbjct:   70 WKFQKVRQVWLLQNMYNKLQVNKHHFKVLLSYLNDLKGISREKTIETARCIVE 122
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A8C3QZJ8_9PASS (Chromosome 7 open reading frame 50 n=1 Tax=Cyanoderma ruficeps TaxID=181631 RepID=A0A8C3QZJ8_9PASS)

HSP 1 Score: 56.6 bits (135), Expect = 1.880e-6
Identity = 27/64 (42.19%), Postives = 39/64 (60.94%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERA----RDLIESGGSTPT 180
            WKF K  Q WLL +MYDK+ V ++ F  +  YL+GLQGG R + +++A    ++   S G  PT
Sbjct:   36 WKFQKTRQTWLLLHMYDKEKVPDKYFTILLDYLQGLQGGARDKTVQKAEAFMKEFDSSDGEDPT 99
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A371DL14_9APHY (Uncharacterized protein n=1 Tax=Polyporus brumalis TaxID=139420 RepID=A0A371DL14_9APHY)

HSP 1 Score: 60.1 bits (144), Expect = 2.410e-6
Identity = 37/103 (35.92%), Postives = 55/103 (53.40%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIESGGSTPTPAQAVP------TPGGPTSGSAPKRSKSDHENKHGAAAAPAGE 291
            WKFNK  Q WL++N++ ++A+ E     + +YL+G+QGGGR+  ++  R+ IE       PA AVP      TP G T   A +  K   E + G+   P  E
Sbjct:  240 WKFNKARQNWLIRNVWSEEAIPEIYVPLVTKYLQGVQGGGRESLIKLCREAIEPPKPPAEPATAVPDAQAEATPDGQTDTPAKRTVKFADEPEEGSKTEPTTE 342
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A6J0H479_9PASS (uncharacterized protein C7orf50 homolog isoform X6 n=4 Tax=Pipridae TaxID=114313 RepID=A0A6J0H479_9PASS)

HSP 1 Score: 57.0 bits (136), Expect = 5.480e-6
Identity = 24/53 (45.28%), Postives = 35/53 (66.04%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIE 159
            WKF K  Q WLL +MYDK+ V ++ F  +  YL+GLQGG R + +E+A   ++
Sbjct:  106 WKFQKARQTWLLLHMYDKEKVPDKYFTILLDYLQGLQGGARDKTVEKAEAFMK 158
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A6J0H476_9PASS (uncharacterized protein C7orf50 homolog isoform X1 n=4 Tax=Pipridae TaxID=114313 RepID=A0A6J0H476_9PASS)

HSP 1 Score: 57.0 bits (136), Expect = 7.070e-6
Identity = 24/53 (45.28%), Postives = 35/53 (66.04%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIE 159
            WKF K  Q WLL +MYDK+ V ++ F  +  YL+GLQGG R + +E+A   ++
Sbjct:  123 WKFQKARQTWLLLHMYDKEKVPDKYFTILLDYLQGLQGGARDKTVEKAEAFMK 175
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A8C5UAV8_9PASS (Chromosome 7 open reading frame 50 n=2 Tax=Passeriformes TaxID=9126 RepID=A0A8C5UAV8_9PASS)

HSP 1 Score: 55.8 bits (133), Expect = 8.130e-6
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIE 159
            WKF K  Q WLL +MYDK+ V ++ F  +  YL+GLQGG R + +++A   ++
Sbjct:   76 WKFQKTRQTWLLLHMYDKEKVPDKYFTILLDYLQGLQGGARDKTVQKAEAFMK 128
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A6J2H3Q3_9PASS (uncharacterized protein C7orf50 homolog n=15 Tax=Passeriformes TaxID=9126 RepID=A0A6J2H3Q3_9PASS)

HSP 1 Score: 57.0 bits (136), Expect = 8.650e-6
Identity = 24/53 (45.28%), Postives = 35/53 (66.04%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIE 159
            WKF K  Q WLL +MYDK+ V ++ F  +  YL+GLQGG R + +E+A   ++
Sbjct:  138 WKFQKARQTWLLLHMYDKEKVPDKYFTILLDYLQGLQGGARDKTVEKAEAFMK 190
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BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Match: A0A812TZ63_9DINO (Hypothetical protein n=1 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812TZ63_9DINO)

HSP 1 Score: 57.0 bits (136), Expect = 8.980e-6
Identity = 27/57 (47.37%), Postives = 37/57 (64.91%), Query Frame = 1 
Query:    1 WKFNKVTQAWLLQNMYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIESGGS 171
            W+FNK TQAWLL++ Y+ + V ++ F+ + RYL GL G  R RAL  A  L+   GS
Sbjct:  105 WRFNKATQAWLLRHAYEPELVPKQVFRILLRYLAGLSGVARDRALAEASTLVTLKGS 161
The following BLAST results are available for this feature:
BLAST of mRNA_S-ischiensis_contig91.20923.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 23
Match NameE-valueIdentityDescription
A0A7M5VAF3_9CNID1.850e-848.15Uncharacterized protein n=1 Tax=Clytia hemisphaeri... [more]
A0A091MK47_9PASS5.880e-745.28Uncharacterized protein C7orf50 (Fragment) n=2 Tax... [more]
T2M730_HYDVU1.650e-649.06Uncharacterized protein C7orf50 n=2 Tax=Hydra vulg... [more]
A0A8C3QZJ8_9PASS1.880e-642.19Chromosome 7 open reading frame 50 n=1 Tax=Cyanode... [more]
A0A371DL14_9APHY2.410e-635.92Uncharacterized protein n=1 Tax=Polyporus brumalis... [more]
A0A6J0H479_9PASS5.480e-645.28uncharacterized protein C7orf50 homolog isoform X6... [more]
A0A6J0H476_9PASS7.070e-645.28uncharacterized protein C7orf50 homolog isoform X1... [more]
A0A8C5UAV8_9PASS8.130e-643.40Chromosome 7 open reading frame 50 n=2 Tax=Passeri... [more]
A0A6J2H3Q3_9PASS8.650e-645.28uncharacterized protein C7orf50 homolog n=15 Tax=P... [more]
A0A812TZ63_9DINO8.980e-647.37Hypothetical protein n=1 Tax=Symbiodinium natans T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-ischiensis_contig91contigS-ischiensis_contig91:450446..453172 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Schizocladia ischiensis KU_03332021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionProtein of unknown function DUF2373
Ec32 orthologEc-22_000160.1
Exons3
Model size1149
Cds size591
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-ischiensis_contig91.20923.1prot_S-ischiensis_contig91.20923.1Schizocladia ischiensis KU_0333polypeptideS-ischiensis_contig91 450962..453130 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622817412.3321893-UTR-S-ischiensis_contig91:450445..4509611622817412.3321893-UTR-S-ischiensis_contig91:450445..450961Schizocladia ischiensis KU_0333UTRS-ischiensis_contig91 450446..450961 -
1695741095.5961554-UTR-S-ischiensis_contig91:450445..4509611695741095.5961554-UTR-S-ischiensis_contig91:450445..450961Schizocladia ischiensis KU_0333UTRS-ischiensis_contig91 450446..450961 -
1622817412.3755453-UTR-S-ischiensis_contig91:453130..4531721622817412.3755453-UTR-S-ischiensis_contig91:453130..453172Schizocladia ischiensis KU_0333UTRS-ischiensis_contig91 453131..453172 -
1695741095.6420248-UTR-S-ischiensis_contig91:453130..4531721695741095.6420248-UTR-S-ischiensis_contig91:453130..453172Schizocladia ischiensis KU_0333UTRS-ischiensis_contig91 453131..453172 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622817412.343459-CDS-S-ischiensis_contig91:450961..4514681622817412.343459-CDS-S-ischiensis_contig91:450961..451468Schizocladia ischiensis KU_0333CDSS-ischiensis_contig91 450962..451468 -
1695741095.6075664-CDS-S-ischiensis_contig91:450961..4514681695741095.6075664-CDS-S-ischiensis_contig91:450961..451468Schizocladia ischiensis KU_0333CDSS-ischiensis_contig91 450962..451468 -
1622817412.3541954-CDS-S-ischiensis_contig91:452833..4528991622817412.3541954-CDS-S-ischiensis_contig91:452833..452899Schizocladia ischiensis KU_0333CDSS-ischiensis_contig91 452834..452899 -
1695741095.6181438-CDS-S-ischiensis_contig91:452833..4528991695741095.6181438-CDS-S-ischiensis_contig91:452833..452899Schizocladia ischiensis KU_0333CDSS-ischiensis_contig91 452834..452899 -
1622817412.3647566-CDS-S-ischiensis_contig91:453112..4531301622817412.3647566-CDS-S-ischiensis_contig91:453112..453130Schizocladia ischiensis KU_0333CDSS-ischiensis_contig91 453113..453130 -
1695741095.628916-CDS-S-ischiensis_contig91:453112..4531301695741095.628916-CDS-S-ischiensis_contig91:453112..453130Schizocladia ischiensis KU_0333CDSS-ischiensis_contig91 453113..453130 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-ischiensis_contig91.20923.1

>prot_S-ischiensis_contig91.20923.1 ID=prot_S-ischiensis_contig91.20923.1|Name=mRNA_S-ischiensis_contig91.20923.1|organism=Schizocladia ischiensis KU_0333|type=polypeptide|length=197bp
MYDKDAVDERGFKRMCRYLEGLQGGGRQRALERARDLIESGGSTPTPAQA
VPTPGGPTSGSAPKRSKSDHENKHGAAAAPAGEEGGGEDGDGEARSADAQ
DRTDAKGKEKKKETNNKKKNSLRVCFAEPLGDSDSPSGPGEPTPTARDSG
DSHGDGSSGLEPRGDTARPEGHAAAAAAARASTVYDRAVCVAEILA*
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mRNA from alignment at S-ischiensis_contig91:450446..453172-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-ischiensis_contig91.20923.1 ID=mRNA_S-ischiensis_contig91.20923.1|Name=mRNA_S-ischiensis_contig91.20923.1|organism=Schizocladia ischiensis KU_0333|type=mRNA|length=2727bp|location=Sequence derived from alignment at S-ischiensis_contig91:450446..453172- (Schizocladia ischiensis KU_0333)
TGGAAGTTCAACAAGGTGACGCAGGCGTGGCTGCTGCAGAACATGTACGA
TAAGGATGCGGTGAGTCGTCGGCTTTGCCCCTCCCCCCCGCTGCATGTCT
CCCTCCCTCCCTCTCCCTCTCTGCCTGTCTCTCTCTCTCTCTCCCTCTCC
CTCTCCCTCTCCCTCTCTGTCTGTCTGTCTGTCTCTCTCCCTCTCTCTCC
CTCTCCCCCCCTCTCTCCCCCTCTCTCTCACGCACACACACACGCACACA
CGCCCCCACATGCGCCCGCTTAGGTGGATGAGCGTGGGTTCAAGAGGATG
TGCAGATACCTGGAGGGCCTGCAGGGGGGAGGGCGACAGGTGCGAATTCT
AGGGTTGTGAACTGATGCCGTTTGTTTACTGCTCAGTACCAGTTAACTGG
TTTAACCGGTCAACCGACCGGTGCCCAATCGTGTAGCCGGAGCGGGTGTC
ATGACCCGTTAAACGTTGATTTTGGCAATACTTTAGCCGTTGCCGGCAAC
CGGTTACAACCAACCGGTCACAAGACCGGTTAGCAGTTAAACTGGTACAG
GCCTTGCCAGTTCACAACCATGGCGGATTCGTTTGCCGGTGCACCCGTGG
GCGGACAGGGGTTTGGTCTATGGCTGTACCGCAATCGTCGGAGAGATCTT
GCCCCCCCGTGCCAATTTTTGGGAGGGCATTTCTCGCCAAATGCTGGTCG
GATTCGCGAGGAAGACTCGGAAAACCTCCCTCAGGACCTGGGGGAACGTT
GTCGAGGGCTTTCGGGACAATCCACGACCAGTCTGGCTAGAAATATTCCC
CCGAGAATTGTCCCGTGGGGGCCAAATCCCTCCGACGATTACAGTACTCG
TAGGCGGTGTGACCTCTCGATAAACGGTGACAACACAGGGCAAGGTGCAG
GCCACGTCACTACTGTAAAGACCTCGACAGTGGTAGTGATGTGATGTGAT
GTGGTTTGTACTCGAATGTTCAGTAGTTTGACATCACACCACATTGAACC
ACATCGAACCACGTCACTAAAGACCGCCACAGTGGCCCTTCCTTAGCTCC
CCAGGTTCAACCTTCCACACTGGAGGTCTTCAGACTCTTTAGTTCCTTAG
CTCGCCAGGCAGCTTCCTTCAATAGCTCGGTGTAGACATTGTGTGGCGGT
TGGATTGACCCAAACATAACAGTCGTGGACCCGTGCGCCTCACAACACAC
ACACCGAGGGAGCAAGGGGGGAGGGGGGGGGATAGACGGAGAGAGAGAGA
GAGAGAGAGAGAGAGAGAGACGGAGACATAGACAATAAGCGTGCACATGC
ACGCGACCACAAACGCACACACAAAGCACAATGTCTAGAGTTTTGAACTG
GAGCCGGTTTTTGCTGGTCGGTTCCAGTTTACTGGTTTAACCGGTTAGCC
GACCGGTGGCCTACCAGTTTCTCCAGTTATTTTCTCCTTTAAAATCGTGC
TCATTTGGCGTTTCGATATAGCCGCTGAGCCCTGGGCTTGCCTGTAAACC
ATGAACGGCTTCGTAAAACTGGTAAAAAACGGCGATAAATGGTTCAGTCA
CCAGTTATCTGGTTCCAACAACATAACTGGTACAAACCGGCCGAACCGAA
CTGCTTACAAGACCAGTTCTCAGTTAAACTGGTACAGGCTGCCCGTTCAC
AACTCCAACAATTTCATCAAGTACACCGTCCCTCCCCCGCCCTCCTGTTT
GCAGCGCGCCCTGGAGCGGGCGCGGGACCTGATTGAGTCCGGCGGGTCCA
CGCCAACGCCCGCCCAGGCCGTACCAACTCCCGGGGGCCCAACCTCAGGC
TCGGCTCCGAAAAGGAGCAAATCCGATCACGAGAATAAGCACGGCGCAGC
GGCAGCTCCGGCCGGCGAAGAGGGGGGCGGGGAGGATGGGGATGGGGAAG
CGAGGAGCGCCGATGCACAGGACAGAACCGACGCAAAAGGCAAGGAGAAG
AAGAAGGAGACGAACAACAAGAAGAAGAATTCGTTGAGGGTCTGCTTCGC
TGAGCCGCTGGGCGACTCAGATAGCCCCTCGGGGCCCGGGGAACCAACCC
CCACAGCTCGAGACTCGGGGGACAGTCACGGCGATGGCAGCAGCGGGCTG
GAGCCTCGAGGTGACACCGCCCGGCCTGAGGGGCATGCCGCCGCCGCCGC
CGCGGCAAGAGCGTCCACGGTATACGACCGGGCCGTGTGTGTTGCTGAGA
TCCTGGCCTGACGATCGGCTCACCGGGTGACTCGGATGACAGCGCGTCGG
GGCCCGGGGAACCGCCCGGGCCCGCGGCTGAAGACCCAGCCCGGGGGGAC
AGTCCGAGTCACTGCAGTGATGGCAGCGGGCTGGAACCTCGAGTTGACAC
CGCCCGGTCTGAGGGGCATGCCGTCCGCGGCAAGAGCGTCCACGGTATAC
GACAGGGCTGTGTGTGTTGCTGAGATCTTGGCCTGATGATCATCGGCAGC
CGGGCCCAAGCGGCTGAAGCCTGAGTAATGAGGACTTTGTCAAGTTCCTG
GATACCTGGATACCTTGGCTACCTTGACTAGTAGGCCAGAGGACGTAGTT
CGACGTCCCCAGACCACCTTAACTACAGTAGAGTCTTTAACTACTGTCAC
TCCGACTCTAATCCTTAACTCTTCTCGGCTTGTCAACAGTGATTACCGTA
ATCGTCGGAGAGATCTTGCCCCCCCATGCCAATATTCGGGAGAACATTTC
TCGCCGAATACTGGTCGGATCTGCGCG
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Coding sequence (CDS) from alignment at S-ischiensis_contig91:450446..453172-

>mRNA_S-ischiensis_contig91.20923.1 ID=mRNA_S-ischiensis_contig91.20923.1|Name=mRNA_S-ischiensis_contig91.20923.1|organism=Schizocladia ischiensis KU_0333|type=CDS|length=1182bp|location=Sequence derived from alignment at S-ischiensis_contig91:450446..453172- (Schizocladia ischiensis KU_0333)
ATGTACGATAAGGATGCGATGTACGATAAGGATGCGGTGGATGAGCGTGG
GTTCAAGAGGATGTGCAGATACCTGGAGGGCCTGCAGGGGGGAGGGCGAC
AGGTGGATGAGCGTGGGTTCAAGAGGATGTGCAGATACCTGGAGGGCCTG
CAGGGGGGAGGGCGACAGCGCGCCCTGGAGCGGGCGCGGGACCTGATTGA
GTCCGGCGGGTCCACGCCAACGCCCGCCCAGGCCGTACCAACTCCCGGGG
GCCCAACCTCAGGCTCGGCTCCGAAAAGGAGCAAATCCGATCACGAGAAT
AAGCACGGCGCAGCGGCAGCTCCGGCCGGCGAAGAGGGGGGCGGGGAGGA
TGGGGATGGGGAAGCGAGGAGCGCCGATGCACAGGACAGAACCGACGCAA
AAGGCAAGGAGAAGAAGAAGGAGACGAACAACAAGAAGAAGAATTCGTTG
AGGGTCTGCTTCGCTGAGCCGCTGGGCGACTCAGATAGCCCCTCGGGGCC
CGGGGAACCAACCCCCACAGCTCGAGACTCGGGGGACAGTCACGGCGATG
GCAGCAGCGGGCTGGAGCCTCGAGGTGACACCGCCCGGCCTGAGGGGCAT
GCCGCCGCCGCCGCCGCGGCAAGAGCGTCCACGGTATACGACCGGGCCGT
GTGTGTTGCTGAGATCCTGGCCTGACGCGCCCTGGAGCGGGCGCGGGACC
TGATTGAGTCCGGCGGGTCCACGCCAACGCCCGCCCAGGCCGTACCAACT
CCCGGGGGCCCAACCTCAGGCTCGGCTCCGAAAAGGAGCAAATCCGATCA
CGAGAATAAGCACGGCGCAGCGGCAGCTCCGGCCGGCGAAGAGGGGGGCG
GGGAGGATGGGGATGGGGAAGCGAGGAGCGCCGATGCACAGGACAGAACC
GACGCAAAAGGCAAGGAGAAGAAGAAGGAGACGAACAACAAGAAGAAGAA
TTCGTTGAGGGTCTGCTTCGCTGAGCCGCTGGGCGACTCAGATAGCCCCT
CGGGGCCCGGGGAACCAACCCCCACAGCTCGAGACTCGGGGGACAGTCAC
GGCGATGGCAGCAGCGGGCTGGAGCCTCGAGGTGACACCGCCCGGCCTGA
GGGGCATGCCGCCGCCGCCGCCGCGGCAAGAGCGTCCACGGTATACGACC
GGGCCGTGTGTGTTGCTGAGATCCTGGCCTGA
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