prot_S-ischiensis_contig11.2020.1 (polypeptide) Schizocladia ischiensis KU_0333

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: A0A6H5KEF6_9PHAE (2-isopropylmalate synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KEF6_9PHAE)

HSP 1 Score: 855 bits (2209), Expect = 2.250e-305
Identity = 439/552 (79.53%), Postives = 495/552 (89.67%), Query Frame = 0

Query:  130 VCVKPTRHDVETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYELSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTGVTDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGTPRQTHPQNDV 681
            V V+P+RHD+ET RDPTRVKVFDTTLRDGEQSPGCTMTREEKL+VA+QLSKLGVDIIEAGFPIAS GDFEAV+EIAT+VG  +NPPIICGLARAL+KDIDVC  AV  ARFPRIHTFIATSDIHMKHKLKK+RE+VL IT+EMVTHARS V+DVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTP EFM LMHHLR TVRGV+ VTISVHGHDDLGMAVANFM+AIEGGARQVECTINGIGERAGNAALEEIVMALHVRK+YYNP+F RPV S+  L+NI+T+EI KSS+LVSSMTGM+VQANKAIVGANAF+HESGIHQDGVLKNKETYEIMDAELVGL Q ++ LVLGKHSGRHAFRSRL E GY L+ DELNRAF+RFK+LADKKKE+T+LDL SIVNDEIR+V N + ++LV +QV AGT N+PTAT+TLYDT  + E  +A  GTGPVDAAYKA+D++ G    KL EYSVASVTAGIDALGEVT RV+D++S R + G++ADTD+IHAS++AY+ A+NRLL+ RG P + HPQ DV
Sbjct:  109 VNVQPSRHDIETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGQGDNPPIICGLARALRKDIDVCANAVAPARFPRIHTFIATSDIHMKHKLKKSREQVLEITREMVTHARSHVDDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFMGLMHHLRGTVRGVDKVTISVHGHDDLGMAVANFMSAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFDRPVLSETALTNINTKEIVKSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLYQDTS-LVLGKHSGRHAFRSRLQESGYSLTDDELNRAFLRFKDLADKKKEITSLDLASIVNDEIRDV-NIRRYELVGMQVAAGTTNKPTATLTLYDTDLEKEGCVAAIGTGPVDAAYKAIDDIVGTKSTKLLEYSVASVTAGIDALGEVTVRVQDELSKREYIGKAADTDVIHASAEAYLHAVNRLLSNRGQPDKAHPQKDV 658          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: A0A835YKD7_9STRA (2-isopropylmalate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YKD7_9STRA)

HSP 1 Score: 798 bits (2061), Expect = 3.800e-284
Identity = 425/590 (72.03%), Postives = 488/590 (82.71%), Query Frame = 0

Query:  100 VSMVTMDNPAAVASASTGPQESPPPLPLKPVCVKPTRHDV--ETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPN-FGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYELSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTG----VTDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGTPRQTHPQNDVS 682
            +SMV MD   +V+S+           P+  V V+P+RHD   ET RDPTRVK+FDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVD+IEAGFPIASPGDFEAV+EIA  VG   NPPIICGLARALKKDIDVC EAV+ ARFPRIHTFIATSDIHM+HKLKKTRE+VLAIT EMV+HA+SL++DVEFSAEDALRSD +FL++V+SVAI AGA+TINVPDTVGYTTP EF +L+H+LR  V G++GVTISVHGH+DLGMAVANF+AAIEGGARQVECT+NGIGERAGNAALEEIVMALHVRK Y+N   F RP +S+  L+N+ T+E++KSSRLVSSMTGM VQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDA LVG+ Q S  LVLGKHSGRHAFRSRL E+GY L+ DELN+AF RFK+LADKKKE++ LDLESIVN+E+RE     AF L  VQVLAG RN PTATV ++D  AD+ES +   GTGPVDA ++A++          D+KL EYSV SVTAGIDALGEVT RV+D  +GR FFGR ADTD+I+AS+ AYV AINR++A R  P   HPQ D S
Sbjct:    1 MSMVIMDPTPSVSSS-----------PVAAVNVRPSRHDTDTETGRDPTRVKIFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDVIEAGFPIASPGDFEAVKEIAETVGTANNPPIICGLARALKKDIDVCAEAVRPARFPRIHTFIATSDIHMEHKLKKTREQVLAITHEMVSHAKSLIDDVEFSAEDALRSDPEFLAKVFSVAIAAGASTINVPDTVGYTTPGEFKSLIHYLREHVAGIDGVTISVHGHNDLGMAVANFLAAIEGGARQVECTVNGIGERAGNAALEEIVMALHVRKGYFNAAAFARPADSERALTNVVTQELYKSSRLVSSMTGMTVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAALVGVVQES-NLVLGKHSGRHAFRSRLAEMGYTLADDELNKAFARFKDLADKKKEISVLDLESIVNEEMREATGHIAFALRGVQVLAGNRNTPTATVIIHDKDADTESHIPAIGTGPVDATFQAINAAVAGHARTGDMKLLEYSVDSVTAGIDALGEVTVRVQDATTGRTFFGRGADTDVIYASAMAYVDAINRIVAARAGPTPKHPQKDTS 578          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: D8LHU2_ECTSI (2-isopropylmalate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHU2_ECTSI)

HSP 1 Score: 789 bits (2038), Expect = 1.080e-281
Identity = 407/516 (78.88%), Postives = 458/516 (88.76%), Query Frame = 0

Query:  166 MTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYELSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTGVTDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGTPRQTHPQNDV 681
            MTREEKL+VA+QL KLGVDIIEAGFPIAS GDFEAV+EIAT+VG   NPPIICGLARALKKDIDVC  AV  ARFPRIHTFIATSDIHMKHKLKK+RE+VL IT+EMVTHARS V+DVEFSAEDALRSDYDFLS+VYSVAIEAGATTINVPDTVGYTTP EFM LMHHLR TVRG++ VTISVHGHDDLGMAVANFM+AIEGGARQVECTINGIGERAGNAALEEIVMALHVRK+YYNP+F RPV S+  L+NI T+EI KSS+LVSSMTGM+VQANKAIVGANAF+HESGIHQDGVLKNKETYEIMDAELVGL Q ++ LVLGKHSGRHAFRSRL E GY L+ DELNRAF+RFK+LADKKKE+T+LDL SIVNDEIR+V N + ++LV +QV AGT N+PTATVTLYDT  + E  +A  GTGPVDAAYKA+D++ G    KL EYSVASVTAGIDALGEVT RV+D++S R + G++ADTD+IHAS++AY+ A+NRLL+ RG P + HPQ DV
Sbjct:    1 MTREEKLMVAKQLGKLGVDIIEAGFPIASQGDFEAVQEIATVVGQGGNPPIICGLARALKKDIDVCANAVAPARFPRIHTFIATSDIHMKHKLKKSREQVLEITREMVTHARSHVDDVEFSAEDALRSDYDFLSKVYSVAIEAGATTINVPDTVGYTTPKEFMGLMHHLRGTVRGIDKVTISVHGHDDLGMAVANFMSAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFDRPVLSETALTNIKTKEIVKSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTS-LVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEITSLDLASIVNDEIRDV-NIRRYELVGMQVAAGTTNKPTATVTLYDTDLEKEGCVAAIGTGPVDAAYKAIDDIVGTRSTKLLEYSVASVTAGIDALGEVTVRVQDELSKREYIGKAADTDVIHASAEAYLHAVNRLLSNRGQPDKAHPQKDV 514          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: A0A4D9D6G1_9STRA (2-isopropylmalate synthase n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D6G1_9STRA)

HSP 1 Score: 764 bits (1974), Expect = 4.620e-271
Identity = 399/546 (73.08%), Postives = 461/546 (84.43%), Query Frame = 0

Query:  134 PTRHDVETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYE-LSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTGVTDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGTPRQTHPQ 678
            PTRHD ET RDP RVK+FDTTLRDGEQSPGCTMT EEKL VA+QL+KLGVD+IEAGFPIAS GDF+AV  IA  VGN ENPPIICGLARAL+KDI+ C  AVK A FPRIHTFIATSDIHM HKLKK+REEVL IT+EMVT+ARSL EDVEFSAEDALRSD  FL +VYSVA+EAGATT+NVPDTVGYTTPSEF AL+  LR  V+GVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKS+YNP FGR  E +  L+NI T EI+K+SRLVS++TGM++Q NKAIVGANAFAHESGIHQDG+LKNK TYEIMDAE +GL +   GLVLGKHSGRHAFRSRLTELGYE LS  ELN+AFVRFKE+ADKKKE+TNLDLESIVN+E+R  +++  ++L+  QV+ G    PTAT+TL +   + E T+A TGTGPVDAAY AV +  G+  +KL E+ V+SVTAGIDALGEVT R+ D  SG++++GRSA+TD++ A++ AY+ A+NR+   RG+    HPQ
Sbjct:   26 PTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALRKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRAHVKGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKN--GLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLEYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVVAATQAYLGALNRVAISRGS-LPVHPQ 568          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: A0A6V1NWK8_HETAK (2-isopropylmalate synthase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1NWK8_HETAK)

HSP 1 Score: 761 bits (1966), Expect = 3.350e-269
Identity = 402/553 (72.69%), Postives = 458/553 (82.82%), Query Frame = 0

Query:  130 VCVKPTRHDVETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYELSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTGVTDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGT--PRQTHPQND 680
            +CVKPT HD+ T RDPTRVKVFDTTLRDGEQSPGCTMT EEK+ VA+QL++LGVD+IEAGFP ASPGDF+AV+ IA  VG  ENPPIICGLARALK DI+ C EAVK A+FPRIHTFIATSDIHM+ KLKKTRE+V+AIT+EMV  ARSLVEDVEFSAEDALRSD DFL EVYSVAIEAGATTINVPDTVGYTTP EF ALMH LR  VRG++ VTISVHGHDDLGMAVANF+AAIEGGARQVECTINGIGERAGNAALEE+VMAL+VRK++YNP FGR  + +  L+N++TREI+K+SRLVSSMTGM+VQ NKAIVGANAFAHESGIHQDGVLKNK TYEIMDAE VGL      LVLGKHSGR AFR+RLTELGYE++ +EL RAF RFK LAD KKE+TN+DLE+IVNDEIR   N + F+L  +QVL G  N PTAT  + DT   SE      GTGPVDAA+K + E   V DVKL EY+V+SVTAGIDALGEVT R+ D  SG+VFFGR+++TD++ AS+ AY+ A NR+   R T   +  HPQ D
Sbjct:   61 ICVKPTAHDLATGRDPTRVKVFDTTLRDGEQSPGCTMTEEEKIAVAKQLARLGVDVIEAGFPFASPGDFKAVKRIAESVGRLENPPIICGLARALKADIEACAEAVKPAKFPRIHTFIATSDIHMEAKLKKTREQVVAITREMVGLARSLVEDVEFSAEDALRSDPDFLCEVYSVAIEAGATTINVPDTVGYTTPDEFYALMHKLRAGVRGIDQVTISVHGHDDLGMAVANFLAAIEGGARQVECTINGIGERAGNAALEEVVMALNVRKAHYNPAFGRATDDERALTNVNTREIYKTSRLVSSMTGMMVQPNKAIVGANAFAHESGIHQDGVLKNKRTYEIMDAETVGLDDNL--LVLGKHSGRAAFRARLTELGYEMAEEELMRAFSRFKVLADVKKEITNMDLEAIVNDEIRADVNDR-FKLDTIQVLCGDANIPTATARILDTQTGSEIVTNSIGTGPVDAAFKCILEAVKVQDVKLQEYAVSSVTAGIDALGEVTVRLLDLTSGKVFFGRASNTDVVVASAQAYINACNRVQISRETLPVKPLHPQRD 610          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: A0A7S2WKU9_9STRA (2-isopropylmalate synthase n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WKU9_9STRA)

HSP 1 Score: 760 bits (1963), Expect = 1.270e-268
Identity = 397/557 (71.27%), Postives = 459/557 (82.41%), Query Frame = 0

Query:  127 LKPVCVKPTRHDVETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYE-LSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTGVTDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGTPRQT-HPQNDV 681
            + PVC +PTRHD+ET RDP RVK+FDTTLRDGEQSPGCTMT EEK+ VARQL++LGVDIIEAGFP AS GDF+AV  IA  VGN E+PPIICGLARA+KKDI+ C  A+++A+FPRIHTFIATSDIHM+HKLKK+R+EVL IT EMV++ARS  EDVEFSAEDALRSD +FL EVYS AIEAGATT+NVPDTVGYTTP EF  L+  +R  VRG+E VTISVHGHDDLGMAVANFM+AIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNP FGRP ES+ PL+NI T+EIFKSSRLVS+MTGM VQ NKAIVGANAFAHESGIHQDGVLKNK TYEIMDAELVGL +    LVLGKHSGRHAFRSRL ELGYE LS +E+ RAFVRFKELADKKKE+TNLD+ES+VNDE +  +    F+L  +QV  G+ + PT           S +T A TGTGPVDAA++A+ ++    D  L EYSV+SVTAGIDALGEVT RVRD++  RV++GRS+D D++ AS+ A++ A+NR +A R    Q  HPQ D+
Sbjct:   67 MHPVCTQPTRHDMETGRDPERVKIFDTTLRDGEQSPGCTMTAEEKIQVARQLARLGVDIIEAGFPFASAGDFDAVSRIAKEVGNLEDPPIICGLARAMKKDIETCARAIEHAKFPRIHTFIATSDIHMEHKLKKSRDEVLDITTEMVSYARSFCEDVEFSAEDALRSDPEFLYEVYSRAIEAGATTLNVPDTVGYTTPREFQTLIEGMRTHVRGIESVTISVHGHDDLGMAVANFMSAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPRFGRPAESETPLTNIMTQEIFKSSRLVSTMTGMAVQPNKAIVGANAFAHESGIHQDGVLKNKATYEIMDAELVGLAENL--LVLGKHSGRHAFRSRLVELGYEKLSEEEIQRAFVRFKELADKKKEITNLDIESLVNDETQ-ANIDDRFKLEQLQVFCGSPSTPTXXXXXXXXXXXSVTTRASTGTGPVDAAFQAIKQIIPAQDCTLLEYSVSSVTAGIDALGEVTCRVRDEVVDRVYYGRSSDNDVVVASAQAFINALNRAVASRDDHSQRFHPQRDI 620          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: A0A7S0VM37_9CRYP (2-isopropylmalate synthase n=2 Tax=Hemiselmis TaxID=77924 RepID=A0A7S0VM37_9CRYP)

HSP 1 Score: 736 bits (1901), Expect = 8.190e-259
Identity = 387/560 (69.11%), Postives = 463/560 (82.68%), Query Frame = 0

Query:  128 KPVCVKPTRHDVETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYELSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTGVT--------DVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGTPR-QTHPQ 678
            KPVCV+PT+HD+ T RDP R+KVFDTTLRDGEQSPGC+MT EEK+ VA+QL+KLGVD+IEAGFPIAS  DF AV  IAT+VGN ENPPIICGLARA K DI+ C +A+  A FPRIHTFIATSDIHM+HKLKKTREEVLAIT EMV++A+S V+DVEFSAEDALRSD+ FL+EVYSVA++AGATT+NVPDTVGYTTP+EF AL+ +LR  V G+EGVTISVHGH+DLGMAV+NF++AIEGGARQVE TINGIGERAGNAALEE+VM L+VRK+YYN   GRP +S+A L+NI+ +EI +SS+LV+++TGM VQ NKAIVGANAFAHESGIHQDG+LKNK TYEI+DA  +GL++   G+VLGKHSGR AFRSRLTELG ELS DELN+AFVRFKE+ADKKKE+TN+D+ESIV DE ++      F+L  VQV  G +   TATVTL D+  D E T A TGTGPVDAA++AV+ L G++         VKL EYSV SVTAGIDALGEVT R+ D  SGR F+GR+++TD++ AS+ AYV A+NRLL  R   + + HPQ
Sbjct:   82 KPVCVEPTKHDLATGRDPARIKVFDTTLRDGEQSPGCSMTSEEKMQVAKQLAKLGVDVIEAGFPIASNDDFAAVNAIATVVGNQENPPIICGLARATKGDIEACAKAISPAAFPRIHTFIATSDIHMEHKLKKTREEVLAITAEMVSYAKSFVDDVEFSAEDALRSDWAFLAEVYSVAVKAGATTLNVPDTVGYTTPAEFKALVEYLREHVEGIEGVTISVHGHNDLGMAVSNFLSAIEGGARQVEVTINGIGERAGNAALEEVVMGLYVRKNYYNERLGRPADSEAELTNINKKEIHRSSKLVTALTGMPVQPNKAIVGANAFAHESGIHQDGMLKNKLTYEIVDASTIGLEKND-GIVLGKHSGRAAFRSRLTELGIELSDDELNKAFVRFKEVADKKKEITNMDIESIVGDEAQDT-TLDRFKLKHVQVQCGDKAIATATVTLMDSQEDVEVTDAATGTGPVDAAFQAVNRLCGLSGAGQACAEQVKLMEYSVTSVTAGIDALGEVTVRLADVKSGRTFYGRASETDVVVASTAAYVHALNRLLVQRSDLQPKLHPQ 639          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: W7TP25_9STRA (2-isopropylmalate synthase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TP25_9STRA)

HSP 1 Score: 746 bits (1927), Expect = 3.000e-252
Identity = 389/521 (74.66%), Postives = 444/521 (85.22%), Query Frame = 0

Query:  134 PTRHDVETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYE-LSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTGVTDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHA 653
            PTRHD ET RDP RVK+FDTTLRDGEQSPGCTMT EEKL VA+QL+KLGVD+IEAGFPIAS GDF+AV  IA  VGN ENPPIICGLARALKKDI+ C  AVK A FPRIHTFIATSDIHM HKLKK+REEVL IT+EMVT+ARSL EDVEFSAEDALRSD  FL +VYSVA+EAGATT+NVPDTVGYTTPSEF AL+  LR  V+GVEGVTISVHGHDDLGMAVANFMAAI GGARQVECTINGIGERAGNAALEEIVMALHVRKS+YNP FGR  E +  L+NI T EI+K+SRLVS++TGM++Q NKAIVGANAFAHESGIHQDG+LKNK TYEIMDAE +GL +   GLVLGKHSGRHAFRSRLTELGYE LS  ELN+AFVRFKE+ADKKKE+TNLDLESIVN+E+R  +++  ++L+  QV+ G    PTAT+TL +   + E T+A TGTGPVDAAY AV +  G+  +KL E+ V+SVTAGIDALGEVT R+ D  SG++++GRSA+TD++ A
Sbjct:  174 PTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKN--GLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSA 692          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: A0A7S0EBN5_9CRYP (2-isopropylmalate synthase n=3 Tax=Geminigeraceae TaxID=589343 RepID=A0A7S0EBN5_9CRYP)

HSP 1 Score: 719 bits (1857), Expect = 4.420e-252
Identity = 382/565 (67.61%), Postives = 463/565 (81.95%), Query Frame = 0

Query:  127 LKPVCVKPTRHDVETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYELSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLYDTAADSESTLAMTGTGPVDAAYKAVDELTGV--------TDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGT-PRQTHPQNDVS 682
            +KPV +KP++HD+ T RDPTRVKVFDTTLRDGEQSPGC+MT +EK+ VA+QL+++GVD+IEAGFPIAS  DF AV++IA +VGN ENPPIICGLARA + DI  C EAVK A+FPRIHTFIATSDIHM+HKL+K+REEVL IT+EMV  A+SLV+DVEFSAEDALRSD  FL++V+SVAI+AGATTINVPDTVG+TTP+EF   + +LR+   G+E VTISVHGHDDLGMAVANF+ AIEGGARQVE T+NGIGERAGNAA+EE+VMALHVRKSYYNP FGRP +S+  L+NI  +EI +SSRLV+S+TGM+VQ NKAIVGANAFAHESGIHQDG+LKNK TYEI+DA  +GL   S G+VLGKHSGR AF++RL +LG  L+ DE N+AF+RFK+LADKKKE+TN DLESIVNDE +++  ++ F+++ VQV  G    PTATVTL D   + E T A TGTGPVDAA++AV+ L G+         DVKL EYSV+SVTAGIDALGEVT R++D  SGRVF GR+ADTD++ AS  AYV A+NRL+  R     + HPQ  V+
Sbjct:   85 MKPVNMKPSKHDLATGRDPTRVKVFDTTLRDGEQSPGCSMTSDEKMQVAKQLARMGVDVIEAGFPIASNDDFIAVKQIADVVGNYENPPIICGLARATEGDIRRCAEAVKGAKFPRIHTFIATSDIHMEHKLRKSREEVLKITEEMVKLAKSLVDDVEFSAEDALRSDRAFLAQVFSVAIKAGATTINVPDTVGFTTPTEFKETIEYLRKHTVGIEDVTISVHGHDDLGMAVANFLGAIEGGARQVEVTVNGIGERAGNAAMEEVVMALHVRKSYYNPMFGRPADSNDALTNIVLKEIHRSSRLVTSLTGMMVQPNKAIVGANAFAHESGIHQDGMLKNKLTYEIIDASTIGLDN-SDGIVLGKHSGRAAFKARLADLGIVLNEDETNKAFIRFKDLADKKKEITNADLESIVNDEAQDLAANR-FKVMHVQVTCGISMVPTATVTLRDEKDNLELTDASTGTGPVDAAFQAVNRLCGIQGGGQANAADVKLMEYSVSSVTAGIDALGEVTVRLQDPRSGRVFLGRAADTDVVVASCVAYVNALNRLILQRSDLAPKLHPQLSVA 647          

BLAST of mRNA_S-ischiensis_contig11.2020.1 vs. uniprot
Match: A0A1X6P6Q5_PORUM (2-isopropylmalate synthase n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6P6Q5_PORUM)

HSP 1 Score: 702 bits (1812), Expect = 1.440e-245
Identity = 378/561 (67.38%), Postives = 441/561 (78.61%), Query Frame = 0

Query:  133 KPTRHDVETRRDPTRVKVFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDIIEAGFPIASPGDFEAVEEIATLVGNTENPPIICGLARALKKDIDVCGEAVKNARFPRIHTFIATSDIHMKHKLKKTREEVLAITKEMVTHARSLVEDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPSEFMALMHHLRRTVRGVEGVTISVHGHDDLGMAVANFMAAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKSYYNPNFGRPVESDAPLSNIDTREIFKSSRLVSSMTGMLVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAELVGLQQTSAGLVLGKHSGRHAFRSRLTELGYELSGDELNRAFVRFKELADKKKELTNLDLESIVNDEIREVHNSQAFQLVAVQVLAGTRNQPTATVTLY--------------DTAADSESTLAMTGTGPVDAAYKAVDELTG-VTDVKLTEYSVASVTAGIDALGEVTARVRDDISGRVFFGRSADTDIIHASSDAYVAAINRLLACRGTPRQTHPQ 678
            KPT+HD+ T RDP RVK+FDTTLRDGEQSPG T+T EEK+++ARQL+KLGVD+IEAGFPIAS GDF AV  IA  VGN  +PPIICGLARAL  DID C EA+K A FPRIHTFIATSD+HM+HKLKK+R EVLAI K MVTHARSL EDVEFSAEDA+RSD +FL EV+SVA+ AGATT+NVPDTVGYTTP EF  L+  +R  V GVE VTISVHGHDDLGMAVANF++A+E GARQ+ECTINGIGERAGNAALEE+VMALHVR++YYN   GR V    PL+NI+  EI+++SRLVSS+TGMLVQ NKAIVGANAF+HESGIHQDGVLKN ETYEIMDA+ VGL+  S  +VLGK SGRHAFRSRLTELGYELS +E+NRAFVRFKELAD KK +++ D+ESIVNDE+R V ++    L  VQV  G  + PT+TVTL               +  AD E T + TGTGPVDA +KA++   G + DV L EY+V+SVT GIDALGEVT RVRD  +GR + G S+DTD++ AS+ AYV AINR       P   HPQ
Sbjct:   69 KPTKHDLATGRDPNRVKIFDTTLRDGEQSPGATLTGEEKMIIARQLAKLGVDVIEAGFPIASVGDFRAVHAIAETVGNRPDPPIICGLARALTADIDRCWEAIKPAAFPRIHTFIATSDVHMEHKLKKSRAEVLAIAKAMVTHARSLCEDVEFSAEDAIRSDPEFLYEVFSVAVAAGATTLNVPDTVGYTTPFEFSKLIRGIRANVVGVENVTISVHGHDDLGMAVANFLSAVESGARQMECTINGIGERAGNAALEELVMALHVRRNYYNSRLGRVV-GGPPLTNINHTEIYQTSRLVSSLTGMLVQPNKAIVGANAFSHESGIHQDGVLKNPETYEIMDAKSVGLKDNS--MVLGKLSGRHAFRSRLTELGYELSDEEINRAFVRFKELADTKKTVSSWDIESIVNDEVRAV-DAPRVALHRVQVSCGNLSVPTSTVTLSVVSGAAMPRMSGSPEPMADGEVTQSATGTGPVDATFKAINAAVGELVDVALEEYTVSSVTKGIDALGEVTVRVRDGATGRRYTGNSSDTDVVVASAMAYVRAINRCTIAAEGPEPIHPQ 625