mRNA_S-ischiensis_contig10.1378.1 (mRNA) Schizocladia ischiensis KU_0333

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-ischiensis_contig10.1378.1
Unique NamemRNA_S-ischiensis_contig10.1378.1
TypemRNA
OrganismSchizocladia ischiensis KU_0333 (Schizocladia ischiensis KU_0333)
Homology
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: A0A7S3UXL7_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UXL7_HETAK)

HSP 1 Score: 85.5 bits (210), Expect = 2.880e-14
Identity = 37/86 (43.02%), Postives = 56/86 (65.12%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRPEVTPAILGMVSEFYQCRGCHKIFWDGPKSASIVRKLSKYVEADAQPP 709
            +V   FGL FR E FMSRC++CN +GF ++  +EA  R  V P +L  V+EF++C GC K++W+GPK ++   K S ++E  +  P
Sbjct:  332 EVVRHFGLVFREEDFMSRCAVCNMKGFAVIAREEAERRG-VKPKVLAKVAEFFECTGCGKVYWEGPKFSTACEKFSCFLEDSSTKP 416
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: A0A7S0S0Y2_9CHLO (Hypothetical protein (Fragment) n=1 Tax=Chlamydomonas leiostraca TaxID=1034604 RepID=A0A7S0S0Y2_9CHLO)

HSP 1 Score: 77.0 bits (188), Expect = 8.530e-13
Identity = 35/72 (48.61%), Postives = 48/72 (66.67%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRPEVTPAILGMVSEFYQCRGCHKIFWDGPKSASIV 667
            +VA  +G+TF     MSRCS+CN+  F L+P +  R+ P V P +L +V EF+QC GC K+FW GPKS S +
Sbjct:  106 EVARQWGITFDTATAMSRCSVCNAADFRLLPRE--RAAPHVPPKVLAVVDEFWQCGGCAKVFWMGPKSYSAI 175
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: H3GHZ0_PHYRM (Mut7-C domain-containing protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3GHZ0_PHYRM)

HSP 1 Score: 73.6 bits (179), Expect = 9.940e-12
Identity = 35/85 (41.18%), Postives = 53/85 (62.35%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRPE--VTPAILGMVSEFYQCRGCHKIFWDGPKSASIVRKLSKYVEADA 700
            ++   F L    E+ MSRCS CN++GFD+V L   R++ E  V P +L +V+EF+ CR CHKI+W+GPK  S    L +  + ++
Sbjct:   78 EIKAHFALKLVKEEMMSRCSRCNAKGFDVVDLDFVRNQTEDAVHPNVLEVVTEFWVCRVCHKIYWEGPKYDSNYANLLRMFDENS 162
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: A0A662WZY2_9STRA (Mut7-C domain-containing protein n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662WZY2_9STRA)

HSP 1 Score: 75.1 bits (183), Expect = 1.980e-11
Identity = 38/96 (39.58%), Postives = 59/96 (61.46%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRP--EVTPAILGMVSEFYQCRGCHKIFWDGPKS----ASIVRKLSKYVEADAQPPCSAS 721
            ++   F L    E+ MSRCS CN++GFD+V L   R++   EV P +L +V+EF+ CR CHKI+W+GPK     A+++R    +   + Q P +A+
Sbjct:  181 EIKTHFALRLVKEEMMSRCSRCNAKGFDVVDLDYVRTQTADEVHPNVLEVVTEFWVCRVCHKIYWEGPKYDSNYANLLRMFDDHSIVEPQHPPNAA 276
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: A0A7S2IFC6_9EUKA (Hypothetical protein n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2IFC6_9EUKA)

HSP 1 Score: 75.5 bits (184), Expect = 4.730e-11
Identity = 37/95 (38.95%), Postives = 48/95 (50.53%), Query Frame = 2 
Query:  437 RTLAAQVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRPEVTPAILGMVSEFYQCRGCHKIFWDGPKSASIVRKLSKYVEADAQPPCSAS 721
            R     V   FGL    ++FM RCS+CN RG+     +EA  R +  P +  MV EFY CR C K++W+GPKS +     S      A    SAS
Sbjct:  310 REQLRSVVSHFGLRLSADEFMMRCSVCNGRGYYKCEKEEAAKREDCPPRVAEMVDEFYVCRSCGKLYWEGPKSNNAFDHFSSVFNGFAAVSTSAS 404
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: A0A225WXL1_9STRA (3'-5' exonuclease domain-containing protein n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225WXL1_9STRA)

HSP 1 Score: 76.3 bits (186), Expect = 5.380e-11
Identity = 36/85 (42.35%), Postives = 53/85 (62.35%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRPE--VTPAILGMVSEFYQCRGCHKIFWDGPKSASIVRKLSKYVEADA 700
            ++   F L    E+ MSRCS CN++GFD+V L   R++ E  V P +L +V+EF+ CR CHKI+W+GPK  S    L +  + D+
Sbjct:  859 EIKTHFALKLVKEEMMSRCSRCNAKGFDIVDLDYVRNQTEDEVHPNVLEIVTEFWVCRVCHKIYWEGPKYDSNYANLLRMFDEDS 943
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: A0A662X4K9_9STRA (Uncharacterized protein n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662X4K9_9STRA)

HSP 1 Score: 75.9 bits (185), Expect = 6.670e-11
Identity = 38/96 (39.58%), Postives = 59/96 (61.46%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRP--EVTPAILGMVSEFYQCRGCHKIFWDGPKS----ASIVRKLSKYVEADAQPPCSAS 721
            ++   F L    E+ MSRCS CN++GFD+V L   R++   EV P +L +V+EF+ CR CHKI+W+GPK     A+++R    +   ++Q P  A+
Sbjct:  720 EIKTHFALRLVKEEMMSRCSRCNAKGFDVVDLDYVRTQTADEVHPNVLEVVTEFWVCRVCHKIYWEGPKYDSNYANLLRMFDDHSIVESQHPSPAA 815
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: A0A329RI35_9STRA (3'-5' exonuclease domain-containing protein n=2 Tax=Phytophthora TaxID=4783 RepID=A0A329RI35_9STRA)

HSP 1 Score: 75.9 bits (185), Expect = 7.410e-11
Identity = 36/85 (42.35%), Postives = 53/85 (62.35%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRPE--VTPAILGMVSEFYQCRGCHKIFWDGPKSASIVRKLSKYVEADA 700
            ++   F L    ++ MSRCS CN++GFD+V L   RS+ E  V P +L +V+EF+ CR CHKI+W+GPK  S    L +  + D+
Sbjct:  957 EIKTHFALKLVKDEMMSRCSRCNAKGFDVVDLDYVRSQTEDEVHPNVLEVVTEFWVCRVCHKIYWEGPKYDSNYANLLRMFDEDS 1041
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BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: W2IDV0_PHYPR (3'-5' exonuclease domain-containing protein n=7 Tax=Phytophthora parasitica TaxID=4792 RepID=W2IDV0_PHYPR)

HSP 1 Score: 74.7 bits (182), Expect = 1.510e-10
Identity = 35/85 (41.18%), Postives = 53/85 (62.35%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRPE--VTPAILGMVSEFYQCRGCHKIFWDGPKSASIVRKLSKYVEADA 700
            ++   F L    ++ MSRCS CN++GFD+V L   R++ E  V P +L +V+EF+ CR CHKI+W+GPK  S    L +  + D+
Sbjct:  676 EIKTHFALKLVKDEMMSRCSRCNAKGFDVVDLDYVRNQTEDEVHPNVLEVVTEFWVCRVCHKIYWEGPKYDSNYANLLRMFDEDS 760
[Close]
BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Match: W2PQF7_PHYPN (3'-5' exonuclease domain-containing protein n=17 Tax=Phytophthora TaxID=4783 RepID=W2PQF7_PHYPN)

HSP 1 Score: 74.7 bits (182), Expect = 1.720e-10
Identity = 35/85 (41.18%), Postives = 53/85 (62.35%), Query Frame = 2 
Query:  452 QVAELFGLTFRPEKFMSRCSLCNSRGFDLVPLQEARSRPE--VTPAILGMVSEFYQCRGCHKIFWDGPKSASIVRKLSKYVEADA 700
            ++   F L    ++ MSRCS CN++GFD+V L   R++ E  V P +L +V+EF+ CR CHKI+W+GPK  S    L +  + D+
Sbjct:  952 EIKTHFALKLVKDEMMSRCSRCNAKGFDVVDLDYVRNQTEDEVHPNVLEVVTEFWVCRVCHKIYWEGPKYDSNYANLLRMFDEDS 1036
The following BLAST results are available for this feature:
BLAST of mRNA_S-ischiensis_contig10.1378.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 18
Match NameE-valueIdentityDescription
A0A7S3UXL7_HETAK2.880e-1443.02Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A7S0S0Y2_9CHLO8.530e-1348.61Hypothetical protein (Fragment) n=1 Tax=Chlamydomo... [more]
H3GHZ0_PHYRM9.940e-1241.18Mut7-C domain-containing protein n=1 Tax=Phytophth... [more]
A0A662WZY2_9STRA1.980e-1139.58Mut7-C domain-containing protein n=1 Tax=Nothophyt... [more]
A0A7S2IFC6_9EUKA4.730e-1138.95Hypothetical protein n=1 Tax=Haptolina brevifila T... [more]
A0A225WXL1_9STRA5.380e-1142.353'-5' exonuclease domain-containing protein n=1 Ta... [more]
A0A662X4K9_9STRA6.670e-1139.58Uncharacterized protein n=1 Tax=Nothophytophthora ... [more]
A0A329RI35_9STRA7.410e-1142.353'-5' exonuclease domain-containing protein n=2 Ta... [more]
W2IDV0_PHYPR1.510e-1041.183'-5' exonuclease domain-containing protein n=7 Ta... [more]
W2PQF7_PHYPN1.720e-1041.183'-5' exonuclease domain-containing protein n=17 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-ischiensis_contig10contigS-ischiensis_contig10:1351252..1353386 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Schizocladia ischiensis KU_03332021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score72.0
Seed ortholog evalue2.9e-10
Seed eggNOG ortholog4792.ETI37896
EggNOG free text desc.Mut7-C RNAse domain
EggNOG OGs3QHVQ@4776,KOG2207@1,KOG2207@2759
COG Functional cat.L
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons2
Model size1377
Cds size501
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-ischiensis_contig10.1378.1prot_S-ischiensis_contig10.1378.1Schizocladia ischiensis KU_0333polypeptideS-ischiensis_contig10 1351632..1352890 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815520.5319142-UTR-S-ischiensis_contig10:1351251..13516311622815520.5319142-UTR-S-ischiensis_contig10:1351251..1351631Schizocladia ischiensis KU_0333UTRS-ischiensis_contig10 1351252..1351631 -
1695739745.9291506-UTR-S-ischiensis_contig10:1351251..13516311695739745.9291506-UTR-S-ischiensis_contig10:1351251..1351631Schizocladia ischiensis KU_0333UTRS-ischiensis_contig10 1351252..1351631 -
1622815520.5696142-UTR-S-ischiensis_contig10:1352890..13533861622815520.5696142-UTR-S-ischiensis_contig10:1352890..1353386Schizocladia ischiensis KU_0333UTRS-ischiensis_contig10 1352891..1353386 -
1695739745.9556515-UTR-S-ischiensis_contig10:1352890..13533861695739745.9556515-UTR-S-ischiensis_contig10:1352890..1353386Schizocladia ischiensis KU_0333UTRS-ischiensis_contig10 1352891..1353386 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815520.5430162-CDS-S-ischiensis_contig10:1351631..13520701622815520.5430162-CDS-S-ischiensis_contig10:1351631..1352070Schizocladia ischiensis KU_0333CDSS-ischiensis_contig10 1351632..1352070 -
1695739745.9394286-CDS-S-ischiensis_contig10:1351631..13520701695739745.9394286-CDS-S-ischiensis_contig10:1351631..1352070Schizocladia ischiensis KU_0333CDSS-ischiensis_contig10 1351632..1352070 -
1622815520.5554192-CDS-S-ischiensis_contig10:1352828..13528901622815520.5554192-CDS-S-ischiensis_contig10:1352828..1352890Schizocladia ischiensis KU_0333CDSS-ischiensis_contig10 1352829..1352890 -
1695739745.947725-CDS-S-ischiensis_contig10:1352828..13528901695739745.947725-CDS-S-ischiensis_contig10:1352828..1352890Schizocladia ischiensis KU_0333CDSS-ischiensis_contig10 1352829..1352890 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-ischiensis_contig10.1378.1

>prot_S-ischiensis_contig10.1378.1 ID=prot_S-ischiensis_contig10.1378.1|Name=mRNA_S-ischiensis_contig10.1378.1|organism=Schizocladia ischiensis KU_0333|type=polypeptide|length=167bp
MSRCSLCNSRGFDLVPLQEARSRPEVTPAILGMVSEFYQCRGCHKIFWDG
PKSASIVRKLSKYVEADAQPPCSASASRAAAAAATAAAAAGAPLSPSSPS
PSLSRAHEGFAVGDSDSGSSDVRPVVWAGGGAVQRLRLADARVSGQAGTH
ERTPPPPGQSPGKRGE*
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mRNA from alignment at S-ischiensis_contig10:1351252..1353386-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-ischiensis_contig10.1378.1 ID=mRNA_S-ischiensis_contig10.1378.1|Name=mRNA_S-ischiensis_contig10.1378.1|organism=Schizocladia ischiensis KU_0333|type=mRNA|length=2135bp|location=Sequence derived from alignment at S-ischiensis_contig10:1351252..1353386- (Schizocladia ischiensis KU_0333)
CAGGGAGTGGTGTGGTAGGCGACAATGCAGAGTAGGCAATGATACAGTAG
GCAGAGTCACCACGTCGCGTACCTGCGTAGGATTGTGAGCAAGCAGGAAA
GGGATCACCGTGTGAGCGTGGCACTCCTATGCTACAATCGCCAGGCACCT
CGCTGATGCCAAGAACATGTACAGTGCTTCCCAAGTGTACATCCGATAAG
TACCGCTCTGGTAATCCAAGCGAGATTTGTTTACTACTCGAGTACAAGTA
TTAGCAGGAACAACACAGGGCGAGGTGCAGGCCGCATCACTAAAGACCTC
AGCAATGGCCCTTCCTTATAGCTCGCGTAGTCGAGCGCCACTGTGACAGA
AAGTGCGAAGTGCTTCGAAGTACTGTTCATTGGAGCAAACCACAGCCGAA
TCAAGCCCCCCGCCCCCGGGCCCATATTGGCACGGGCGGACTTTGGCGGC
ACAGGTGGCGGAGCTGTTCGGACTGACCTTCCGCCCCGAGAAGTTCATGA
GCCGCTGCTCCCTGTGCAACTCCAGGGGGTTTGACCTCGTCCCGCTGCAG
GAGGCCAGGTGAGCAAACCAATGTGCTCCCAGGTAGCAGCACAGCAGACA
CGGTGTCGTGGATTTGGGGGTCTCTCCTAGGGGGTGTCTCACCTAGCTAG
GGGTCTCTCCTAATCCACGACAGCAGAGCAAACCGCCCTCCTCCAGCCGG
GTGCTCTGCATCTGTAGTCACCGATTGCGATGATAGCTTTGGCACTCACA
CAAAGTAAGTACGAGTATGCAAGTAGTGGAAGCGTACTCTACACAATGTA
CTACACGTGCTTCCGGATTTGTCTTAGAACAAACCACAGAGAGGTGCGGC
CCAGGTCACTGATCACCTCAACAGCGGTACAGGCCTTCCTTTGCTCGCAA
CACGTGCTCAACTCTATGTCGTTATGCCTCGGGCGTGGCCCCTCCGGGGG
TGGCGGGGTCCTGGTGGGGGCAGGGGGCATCGTGAACCAGGAGACATGCC
GAGCTGTCTGGCCGCACAGTCTACCGCCGTGCAGCTGGGACGTGCAAGTG
CACGCGTTGGTGTATGCTTCCAGTACAATAGGTAGAGTATATACACGAGT
CCCACATACAGTCCACAGCCGTGCTGGCACTCATACCAACTGTTTCCTCT
TTGCTACAGTATGTAAGTGAAATAGAGTATATTTGTACAGCTTGGTAGCT
TTGTGCACCGTCATCCTCTAGAGCCGGGCAAGGACTAAGGACACCCGCAA
CCTCGTTCGCCCCCTTTTCCCGCCCGTACCCCGGCCCGGGGGACTCGGGG
GGGGGGGTCCGCTCAGGTCCCGGCCAGAGGTGACACCGGCCATCCTCGGC
ATGGTCTCCGAGTTCTACCAGTGCCGCGGCTGCCACAAGATATTCTGGGA
CGGGCCCAAGAGCGCTTCCATCGTGCGCAAGCTGTCCAAATACGTCGAGG
CTGACGCCCAGCCGCCATGCTCTGCCAGTGCTTCCCGGGCGGCGGCGGCG
GCGGCGACGGCGGCGGCAGCGGCGGGGGCTCCGCTGAGTCCCAGCTCTCC
GAGTCCAAGCCTGAGTCGGGCACATGAGGGGTTCGCAGTCGGCGACTCGG
ACAGTGGCAGTAGTGACGTGCGGCCAGTGGTATGGGCGGGCGGCGGCGCC
GTTCAAAGGCTTCGGCTCGCCGACGCCCGGGTATCTGGGCAGGCTGGGAC
ACATGAGCGGACACCCCCACCCCCCGGCCAGTCTCCTGGGAAACGGGGCG
AGTGAAGTAGGGTGCCCCTCGACCGGGCCGGCGGGGTCGGGTGTGCCAGC
GGGGAGGTGACTCGGGTGACCTCTTGCGTGTGTGGCGGCGGCGCTCAGGA
TGGAAGCTGCTGATGGTGGTGGCAAAGAGGAGGAGGAGGAGCAAACTATA
GGAAAAATTGAGATAGATCCACCACAGCCAGTGCACCTGTGCAATCGGCA
GCGTCATCTGCACTTCCGGAAAGACGGTGGTGCTGTGCCGCTTGCATGCA
GGTGCCGCAAGCTTGGCGTGATTACAGTACTGCACTGTTTGGGCTGGGCG
AGCACGTCGTGGCTATTACACAGACAGAGAACGTACAGTACAAGCAGGCA
ATTAGCGGCTCCCCTAATCCACGACACGGTGTCAC
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Coding sequence (CDS) from alignment at S-ischiensis_contig10:1351252..1353386-

>mRNA_S-ischiensis_contig10.1378.1 ID=mRNA_S-ischiensis_contig10.1378.1|Name=mRNA_S-ischiensis_contig10.1378.1|organism=Schizocladia ischiensis KU_0333|type=CDS|length=1002bp|location=Sequence derived from alignment at S-ischiensis_contig10:1351252..1353386- (Schizocladia ischiensis KU_0333)
ATGAGCCGCTGCTCCCTGTGCAACTCCAGGGGGTTTGACCTCGTCCCGCT
GCAGGAGGCCAGATGAGCCGCTGCTCCCTGTGCAACTCCAGGGGGTTTGA
CCTCGTCCCGCTGCAGGAGGCCAGGTCCCGGCCAGAGGTGACACCGGCCA
TCCTCGGCATGGTCTCCGAGTTCTACCAGTGCCGCGGCTGCCACAAGATA
TTCTGGGACGGGCCCAAGAGCGCTTCCATCGTGCGCAAGCTGTCCAAATA
CGTCGAGGCTGACGCCCAGCCGCCATGCTCTGCCAGTGCTTCCCGGGCGG
CGGCGGCGGCGGCGACGGCGGCGGCAGCGGCGGGGGCTCCGCTGAGTCCC
AGCTCTCCGAGTCCAAGCCTGAGTCGGGCACATGAGGGGTTCGCAGTCGG
CGACTCGGACAGTGGCAGTAGTGACGTGCGGCCAGTGGTATGGGCGGGCG
GCGGCGCCGTTCAAAGGCTTCGGCTCGCCGACGCCCGGGTATCTGGGCAG
GCTGGGACACATGAGCGGACACCCCCACCCCCCGGCCAGTCTCCTGGGAA
ACGGGGCGAGTGAGTCCCGGCCAGAGGTGACACCGGCCATCCTCGGCATG
GTCTCCGAGTTCTACCAGTGCCGCGGCTGCCACAAGATATTCTGGGACGG
GCCCAAGAGCGCTTCCATCGTGCGCAAGCTGTCCAAATACGTCGAGGCTG
ACGCCCAGCCGCCATGCTCTGCCAGTGCTTCCCGGGCGGCGGCGGCGGCG
GCGACGGCGGCGGCAGCGGCGGGGGCTCCGCTGAGTCCCAGCTCTCCGAG
TCCAAGCCTGAGTCGGGCACATGAGGGGTTCGCAGTCGGCGACTCGGACA
GTGGCAGTAGTGACGTGCGGCCAGTGGTATGGGCGGGCGGCGGCGCCGTT
CAAAGGCTTCGGCTCGCCGACGCCCGGGTATCTGGGCAGGCTGGGACACA
TGAGCGGACACCCCCACCCCCCGGCCAGTCTCCTGGGAAACGGGGCGAGT
GA
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