mRNA_S-dermatodea_contig11077.705.1 (mRNA) Saccorhiza dermatodea SderLu1190fm monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-dermatodea_contig11077.705.1
Unique NamemRNA_S-dermatodea_contig11077.705.1
TypemRNA
OrganismSaccorhiza dermatodea SderLu1190fm monoicous (Saccorhiza dermatodea SderLu1190fm monoicous)
Homology
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BLAST of mRNA_S-dermatodea_contig11077.705.1 vs. uniprot
Match: A0A6H5L472_9PHAE (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L472_9PHAE)

HSP 1 Score: 338 bits (866), Expect = 7.000e-107
Identity = 250/397 (62.97%), Postives = 298/397 (75.06%), Query Frame = 1 
Query:   25 SSRLDSIVTQLQSEIESGATPQVQSRVNLDSLFADLRPPSSALTSLSPTTTSSSPGIPTAAGINSDQPEKPPPASDTSGGLKPSPST--ATPGGGQDLESLSDMLGRIQANAKARDLADSRTRAAALRSGGPLSEG--LSTLTLSKHKELKTRFASIRQGRVSWVVLPMMRRARKAGIPLSTGVYNAAIAAYLGTPSKYEDALRVLELLRNSGDPDVGPDLKSYNIAMRVCGEAGKWRVVFKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLVEAAGMGSISPRQNMIQIVYDMEQAGVEPTKYTYTLLLDCLAQRGKAFDGFQVMARLVDARAPLLPLGYRAGLLLCKEYGDWRRAMVLMEDMRVSKKRVS 1203
            SSRL  +V+QLQ E+ +G+TPQV+S + LD LFADLR         + TTT       T  G N+  P+ P        G   +  +  A   G  DLESLSDML R+QA+AKARDLA++  R+ ALRSGGP+ +    S +T+   +++KTRF +IR+GRV W VLP++ +A++AGIPLSTGVYNAAIAAY GTP KYEDALRVL +LR   DP V PDL SYN AM VC EAG+WR+V +    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLVEAAGMGS +PR++MI++V++MEQAGVEP  YT+T LL CLA+R K FDGFQ  ARLVDARAPLLP G+RAGL  C  YGDWRRAM+LMEDMRV+K+R S
Sbjct:  115 SSRLQDVVSQLQGELRAGSTPQVRSSIKLDGLFADLRGQEDTPRGRNSTTT-------TTTGPNTIPPKSPKQQRRLPNGKLSAAGSGDAALEGTDDLESLSDMLDRVQASAKARDLAEAEARSLALRSGGPIGDDPVRSWVTIGNQRDIKTRFRNIREGRVGWAVLPLVMKAKQAGIPLSTGVYNAAIAAYAGTPRKYEDALRVLNMLRREEDPGVRPDLGSYNAAMWVCSEAGQWRLVMELMTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLVEAAGMGSAAPRKHMIEVVHEMEQAGVEPNSYTFTTLLICLARRRKTFDGFQ--ARLVDARAPLLPHGHRAGLQFCDAYGDWRRAMILMEDMRVAKRRPS 502
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BLAST of mRNA_S-dermatodea_contig11077.705.1 vs. uniprot
Match: D7FLV0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FLV0_ECTSI)

HSP 1 Score: 345 bits (885), Expect = 3.630e-106
Identity = 254/405 (62.72%), Postives = 300/405 (74.07%), Query Frame = 1 
Query:   25 SSRLDSIVTQLQSEIESGATPQVQSRVNLDSLFADLR---PPSSALTSLSPTTTSSSPGIPTAAGINSDQPEKPPPASDTSGGLKPSPSTATPGGGQ-------DLESLSDMLGRIQANAKARDLADSRTRAAALRSGGPLSE--GLSTLTLSKHKELKTRFASIRQGRVSWVVLPMMRRARKAGIPLSTGVYNAAIAAYLGTPSKYEDALRVLELLRNSGDPDVGPDLKSYNIAMRVCGEAGKWRVVFKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLVEAAGMGSISPRQNMIQIVYDMEQAGVEPTKYTYTLLLDCLAQRGKAFDGFQVMARLVDARAPLLPLGYRAGLLLCKEYGDWRRAMVLMEDMRVSKKRVS 1203
            SSRL  +V+QLQ E+ +G TPQV+S + LDSLFADLR     S    S +PTTT                P+  PP S       PS      G G        DLESLS+ML R+QA+AKARDLA++  R+  LRSGGP+ +    S +T+   +++KTRF +IR+GRV W VLP++ +A++AGIPLSTGVYNAAIAAY GTP KYEDALRVL +LR   DP V PDL SYN AM VC EAG+WR+V +    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLVEAAGMGS +PR++MI++V++MEQAGVEP  YT+T LL+CLA+R K FDGFQVMARLVDARAPLLP G+RAGL  C  YGDWRRAM+LMEDMRV+K+R S
Sbjct:   65 SSRLQDVVSQLQGELRAGTTPQVRSSIKLDSLFADLRGQEDTSRGRNSKTPTTTG---------------PKTIPPKSPKQPRRLPSGKFCAAGSGDAALQETDDLESLSNMLDRVQASAKARDLAEAEARSLELRSGGPIGDDPARSWVTIGNQRDMKTRFRNIREGRVGWAVLPLVMKAKQAGIPLSTGVYNAAIAAYSGTPRKYEDALRVLNMLRREEDPGVRPDLGSYNAAMWVCSEAGQWRLVMELMTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLVEAAGMGSAAPRKHMIEVVHEMEQAGVEPNSYTFTTLLNCLARRRKIFDGFQVMARLVDARAPLLPHGHRAGLQFCDAYGDWRRAMILMEDMRVAKRRPS 454
The following BLAST results are available for this feature:
BLAST of mRNA_S-dermatodea_contig11077.705.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
A0A6H5L472_9PHAE7.000e-10762.97Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]
D7FLV0_ECTSI3.630e-10662.72Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-dermatodea_contig11077contigS-dermatodea_contig11077:4680..7417 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Saccorhiza dermatodea SderLu1190fm monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score350.1
Seed ortholog evalue1.3e-93
Seed eggNOG ortholog2880.D7FLV0
KEGG koko:K17710
EggNOG free text desc.endonuclease activity
EggNOG OGsKOG4197@1,KOG4197@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03016,ko03029
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionPentatricopeptide repeat
Ec32 orthologEc-26_001150.1
Exons4
Model size1207
Cds size1203
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-dermatodea_contig11077.705.1prot_S-dermatodea_contig11077.705.1Saccorhiza dermatodea SderLu1190fm monoicouspolypeptideS-dermatodea_contig11077 4680..7415 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622924186.2656753-CDS-S-dermatodea_contig11077:4679..54171622924186.2656753-CDS-S-dermatodea_contig11077:4679..5417Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig11077 4680..5417 +
1694079077.310982-CDS-S-dermatodea_contig11077:4679..54171694079077.310982-CDS-S-dermatodea_contig11077:4679..5417Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig11077 4680..5417 +
1622924186.2769349-CDS-S-dermatodea_contig11077:6224..64401622924186.2769349-CDS-S-dermatodea_contig11077:6224..6440Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig11077 6225..6440 +
1694079077.323194-CDS-S-dermatodea_contig11077:6224..64401694079077.323194-CDS-S-dermatodea_contig11077:6224..6440Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig11077 6225..6440 +
1622924186.284266-CDS-S-dermatodea_contig11077:6682..67871622924186.284266-CDS-S-dermatodea_contig11077:6682..6787Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig11077 6683..6787 +
1694079077.3327224-CDS-S-dermatodea_contig11077:6682..67871694079077.3327224-CDS-S-dermatodea_contig11077:6682..6787Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig11077 6683..6787 +
1622924186.2929049-CDS-S-dermatodea_contig11077:7271..74151622924186.2929049-CDS-S-dermatodea_contig11077:7271..7415Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig11077 7272..7415 +
1694079077.342681-CDS-S-dermatodea_contig11077:7271..74151694079077.342681-CDS-S-dermatodea_contig11077:7271..7415Saccorhiza dermatodea SderLu1190fm monoicousCDSS-dermatodea_contig11077 7272..7415 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-dermatodea_contig11077.705.1

>prot_S-dermatodea_contig11077.705.1 ID=prot_S-dermatodea_contig11077.705.1|Name=mRNA_S-dermatodea_contig11077.705.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=polypeptide|length=401bp
EDQASLASSSRLDSIVTQLQSEIESGATPQVQSRVNLDSLFADLRPPSSA
LTSLSPTTTSSSPGIPTAAGINSDQPEKPPPASDTSGGLKPSPSTATPGG
GQDLESLSDMLGRIQANAKARDLADSRTRAAALRSGGPLSEGLSTLTLSK
HKELKTRFASIRQGRVSWVVLPMMRRARKAGIPLSTGVYNAAIAAYLGTP
SKYEDALRVLELLRNSGDPDVGPDLKSYNIAMRVCGEAGKWRVVFKLLKE
MEEDGIKPDTSSYNCALNGLAKCREWYRARRLFRKMGATGLTPDTYSFNG
LVEAAGMGSISPRQNMIQIVYDMEQAGVEPTKYTYTLLLDCLAQRGKAFD
GFQVMARLVDARAPLLPLGYRAGLLLCKEYGDWRRAMVLMEDMRVSKKRV
S
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mRNA from alignment at S-dermatodea_contig11077:4680..7417+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-dermatodea_contig11077.705.1 ID=mRNA_S-dermatodea_contig11077.705.1|Name=mRNA_S-dermatodea_contig11077.705.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=mRNA|length=2738bp|location=Sequence derived from alignment at S-dermatodea_contig11077:4680..7417+ (Saccorhiza dermatodea SderLu1190fm monoicous)
GAGGACCAGGCGTCTCTGGCCAGTAGCTCGCGTCTGGACAGCATTGTGAC
CCAGCTCCAGAGCGAAATCGAGTCCGGCGCGACTCCTCAAGTCCAATCTC
GTGTCAACCTAGATAGCCTGTTCGCGGACCTCCGACCGCCGAGCTCAGCG
TTAACGTCACTATCACCAACGACGACAAGCTCCTCCCCCGGGATTCCGAC
TGCGGCGGGAATCAACAGCGACCAGCCAGAGAAGCCCCCCCCTGCCAGTG
ACACCTCGGGTGGTTTGAAACCAAGCCCGAGCACCGCCACTCCAGGAGGG
GGGCAAGACCTAGAGTCCTTGTCCGATATGCTGGGCCGGATTCAGGCCAA
CGCGAAGGCTAGAGACCTCGCCGATTCGCGAACACGGGCGGCCGCTCTCA
GGTCGGGCGGCCCCCTCAGCGAAGGTCTCTCCACCCTGACGCTGTCAAAG
CACAAGGAGCTCAAGACGCGGTTCGCTAGCATCCGCCAGGGGAGGGTGAG
CTGGGTCGTGCTGCCTATGATGAGGCGCGCGCGCAAGGCGGGCATACCGT
TAAGCACCGGGGTATACAACGCGGCGATCGCTGCGTACCTCGGCACACCG
AGCAAGTACGAGGACGCGTTGCGGGTTCTGGAGCTATTGAGGAACTCGGG
GGACCCTGATGTAGGGCCGGACCTCAAGTCGTACAACATCGCGATGCGGG
TGTGCGGGGAGGCTGGCAAGTGGCGGGTTGTGTTCAAGGTGGGCCCTGCT
GTAGAAGTCTGTGACGGAATACTGGTATGGTAGGCAGTAGGCAGGTGTCA
TAACCGTCCTCTTCAGAATTACGTCACGCTGTCTGCTCTGTGGACGGATA
CGTAACGGCAAACTCCCGTGCCTCCGCTTCAAGTTGTGAGTTTCAAAAGC
ATCGGAGTGCTCTCACATTGAGGGTTAGGTCCATGGTGGATGTATTGTTG
TCTGGATAGGCGAGAGTCTGCCGCCTCCAGAGCTCAGTAAATGTTACCAG
CGGCGATGCAGAGTAAGCCACTTAGCTCTACATGCCTGCCTTGCTTGTTG
CAATGTCCAGCTTCTTTGCGGAGCCGGGGTGTTGTTGTCAGTGCTATGTC
CTACTACAGCGGCAATGTAGATCTAGAGCTCTAAGCCAAGAAAAAAAAAT
GCTTCTTGTTGTGCAGCAGAGCTCGCCGCTCGACTACAAATATCTTCATC
AGGCGCAAACAGCATGAGCTTCGTAAGATAACCACCGCCAGCTGCTTTCC
AGGCCAACCATGTTCAGCACAAGACAGCTATGATACTCGTTGCATCGCCT
CTCCTAAACAGGGCTGTACAAAGTTACCCAGCAGCGGTACAGCCAGCAGT
CTGCACCTCTCAACATCGCCGATAACCCGAACAGCGAAAGCTTGATCGCA
AAACCACGAAGCACCGTTGTCCTTGCCCTGCGCTAATGCGCCTCCCCTCG
AAAAGAACCAAGGCATACACTCACACTCCCAGCCCCCCCCCCGCCCCCGC
CCCCGCCCACGCCGCCATTCCCACGTACCAAACAACGACGAGCAGCTGCT
GAAGGAGATGGAGGAGGACGGCATAAAGCCCGACACATCATCGTACAACT
GCGCGTTGAACGGACTGGCGAAATGTCGGGAGTGGTATCGTGCTCGCCGA
CTGTTCCGCAAGATGGGTGCGACCGGCCTGACTCCTGATACGTACAGCTT
CAACGGGCTGGTGGAGGCTGCAGGGATGGGGAGCATCTCTCCCAGACAAA
ACATGATCCAGGTGAACGTGGTGCCTTGCCGCCCTTAGACGAAGCGACCG
TTTTTTGTCGCTATTTCTCCCCGACGATTTCGGCTTTCGTAGCGCTACTT
ATGGTTGGTCGTTCTCGTGTCTTCACAGCAGAGCGTGCTACACCTCTCAC
GTAAAAATTTGTCCTGGATAAAAACCTGACGCAGCACCAAATCGGAGTGA
TCTTGAGGCCTCGTCGTTCCGTCTCCACCCCTTGCCTTGCGCCTTGTGCT
CAGATCGTGTACGACATGGAGCAGGCAGGAGTGGAGCCAACGAAGTACAC
CTACACGCTGCTGCTGGACTGCTTGGCTCAGAGGGGCAAGGCTTTTGACG
GCTTTCAGGTGAGCAGATCTCGATGCTAGCGAGAGCGTGCTGCTCATGTA
TGTAAGACCTCGCGGTTCCGCCTATGGTCGACACGGCTCGGCAGCGTAAG
AGTTGGGAGAACGCCTCTCTTTTGTGCCCGCCTTAAGTAACCGGAGGGTG
TCTACACCTTGAGCCGCCACTCTCGCCAATTGTTTTCCGCTAGGAACAAT
TGGACGCGTGGCTCAGGGCGCCACATGAGCTCAGAGTCCGCCTGCGTCAT
GCCGGCAATCGTCCTCATTATAGTGTATCCAAAATGAAATTTAATTTGCT
GCCCGCGTCCGCAGCACCCGTTGGCAAGATCTTGCCTACAGCAACGTCGC
TTGCTCTACGGTTGTGTCGTTGAACGTAACGATCGAGCATCCCCCTGAGT
AACCGCGGCCTACCTGTCCAGTTTGGCTGGGCAAAACCTGATTTTCGTTG
TATCAATTGTGCTGTATTTCGTGTATTTCTTGTGGCCGGCAGGTTATGGC
GCGTCTGGTGGACGCTCGCGCCCCGCTTCTACCGCTGGGATACCGCGCCG
GGCTGCTGCTCTGCAAAGAGTACGGGGACTGGCGCCGCGCTATGGTGTTA
ATGGAGGATATGCGGGTTTCCAAAAAGCGCGTTTCGGG
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Coding sequence (CDS) from alignment at S-dermatodea_contig11077:4680..7417+

>mRNA_S-dermatodea_contig11077.705.1 ID=mRNA_S-dermatodea_contig11077.705.1|Name=mRNA_S-dermatodea_contig11077.705.1|organism=Saccorhiza dermatodea SderLu1190fm monoicous|type=CDS|length=2406bp|location=Sequence derived from alignment at S-dermatodea_contig11077:4680..7417+ (Saccorhiza dermatodea SderLu1190fm monoicous)
GAGGACCAGGCGTCTCTGGCCAGTAGCTCGCGTCTGGACAGCATTGTGAC
CCAGCTCCAGAGCGAAATCGAGTCCGGCGCGACTCCTCAAGTCCAATCTC
GTGTCAACCTAGATAGCCTGTTCGCGGACCTCCGACCGCCGAGCTCAGCG
TTAACGTCACTATCACCAACGACGACAAGCTCCTCCCCCGGGATTCCGAC
TGCGGCGGGAATCAACAGCGACCAGCCAGAGAAGCCCCCCCCTGCCAGTG
ACACCTCGGGTGGTTTGAAACCAAGCCCGAGCACCGCCACTCCAGGAGGG
GGGCAAGACCTAGAGTCCTTGTCCGATATGCTGGGCCGGATTCAGGCCAA
CGCGAAGGCTAGAGACCTCGCCGATTCGCGAACACGGGCGGCCGCTCTCA
GGTCGGGCGGCCCCCTCAGCGAAGGTCTCTCCACCCTGACGCTGTCAAAG
CACAAGGAGCTCAAGACGCGGTTCGCTAGCATCCGCCAGGGGAGGGTGAG
CTGGGTCGTGCTGCCTATGATGAGGCGCGCGCGCAAGGCGGGCATACCGT
TAAGCACCGGGGTATACAACGCGGCGATCGCTGCGTACCTCGGCACACCG
AGCAAGTACGAGGACGCGTTGCGGGTTCTGGAGCTATTGAGGAACTCGGG
GGACCCTGATGTAGGGCCGGACCTCAAGTCGTACAACATCGCGATGCGGG
TGTGCGGGGAGGCTGGCAAGTGGCGGGTTGTGTTCAAGGAGGACCAGGCG
TCTCTGGCCAGTAGCTCGCGTCTGGACAGCATTGTGACCCAGCTCCAGAG
CGAAATCGAGTCCGGCGCGACTCCTCAAGTCCAATCTCGTGTCAACCTAG
ATAGCCTGTTCGCGGACCTCCGACCGCCGAGCTCAGCGTTAACGTCACTA
TCACCAACGACGACAAGCTCCTCCCCCGGGATTCCGACTGCGGCGGGAAT
CAACAGCGACCAGCCAGAGAAGCCCCCCCCTGCCAGTGACACCTCGGGTG
GTTTGAAACCAAGCCCGAGCACCGCCACTCCAGGAGGGGGGCAAGACCTA
GAGTCCTTGTCCGATATGCTGGGCCGGATTCAGGCCAACGCGAAGGCTAG
AGACCTCGCCGATTCGCGAACACGGGCGGCCGCTCTCAGGTCGGGCGGCC
CCCTCAGCGAAGGTCTCTCCACCCTGACGCTGTCAAAGCACAAGGAGCTC
AAGACGCGGTTCGCTAGCATCCGCCAGGGGAGGGTGAGCTGGGTCGTGCT
GCCTATGATGAGGCGCGCGCGCAAGGCGGGCATACCGTTAAGCACCGGGG
TATACAACGCGGCGATCGCTGCGTACCTCGGCACACCGAGCAAGTACGAG
GACGCGTTGCGGGTTCTGGAGCTATTGAGGAACTCGGGGGACCCTGATGT
AGGGCCGGACCTCAAGTCGTACAACATCGCGATGCGGGTGTGCGGGGAGG
CTGGCAAGTGGCGGGTTGTGTTCAAGCTGCTGAAGGAGATGGAGGAGGAC
GGCATAAAGCCCGACACATCATCGTACAACTGCGCGTTGAACGGACTGGC
GAAATGTCGGGAGTGGTATCGTGCTCGCCGACTGTTCCGCAAGATGGGTG
CGACCGGCCTGACTCCTGATACGTACAGCTTCAACGGGCTGGTGGAGGCT
GCAGGGATGGGGAGCATCTCTCCCAGACAAAACATGATCCAGCTGCTGAA
GGAGATGGAGGAGGACGGCATAAAGCCCGACACATCATCGTACAACTGCG
CGTTGAACGGACTGGCGAAATGTCGGGAGTGGTATCGTGCTCGCCGACTG
TTCCGCAAGATGGGTGCGACCGGCCTGACTCCTGATACGTACAGCTTCAA
CGGGCTGGTGGAGGCTGCAGGGATGGGGAGCATCTCTCCCAGACAAAACA
TGATCCAGATCGTGTACGACATGGAGCAGGCAGGAGTGGAGCCAACGAAG
TACACCTACACGCTGCTGCTGGACTGCTTGGCTCAGAGGGGCAAGGCTTT
TGACGGCTTTCAGATCGTGTACGACATGGAGCAGGCAGGAGTGGAGCCAA
CGAAGTACACCTACACGCTGCTGCTGGACTGCTTGGCTCAGAGGGGCAAG
GCTTTTGACGGCTTTCAGGTTATGGCGCGTCTGGTGGACGCTCGCGCCCC
GCTTCTACCGCTGGGATACCGCGCCGGGCTGCTGCTCTGCAAAGAGTACG
GGGACTGGCGCCGCGCTATGGTGTTAATGGAGGATATGCGGGTTTCCAAA
AAGCGCGTTTCGGTTATGGCGCGTCTGGTGGACGCTCGCGCCCCGCTTCT
ACCGCTGGGATACCGCGCCGGGCTGCTGCTCTGCAAAGAGTACGGGGACT
GGCGCCGCGCTATGGTGTTAATGGAGGATATGCGGGTTTCCAAAAAGCGC
GTTTCG
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