mRNA_P-littoralis_Contig130.61.1 (mRNA) Pylaiella littoralis U1_48

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-littoralis_Contig130.61.1
Unique NamemRNA_P-littoralis_Contig130.61.1
TypemRNA
OrganismPylaiella littoralis U1_48 (Pylaiella littoralis U1_48 (Mung))
Homology
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BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: A0A6H5K2A1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K2A1_9PHAE)

HSP 1 Score: 191 bits (486), Expect = 1.140e-55
Identity = 105/129 (81.40%), Postives = 106/129 (82.17%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD 388
            ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV+
Sbjct:   65 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVE 171
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BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: A0A6S8BTP7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A6S8BTP7_9STRA)

HSP 1 Score: 187 bits (474), Expect = 1.610e-55
Identity = 103/129 (79.84%), Postives = 104/129 (80.62%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD 388
            ESTLHLVLR RGGMQIFVKTLTGKTITLDVEPSDTID VKQKIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV+
Sbjct:    4 ESTLHLVLRRRGGMQIFVKTLTGKTITLDVEPSDTIDNVKQKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVE 110
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BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: A0A7S1UDB6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UDB6_9STRA)

HSP 1 Score: 189 bits (480), Expect = 5.820e-55
Identity = 104/129 (80.62%), Postives = 105/129 (81.40%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD 388
            ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTID VKQKIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV+
Sbjct:   48 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDNVKQKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVE 154
[Close]
BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: A0A7S4VZT1_9STRA (Hypothetical protein (Fragment) n=3 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4VZT1_9STRA)

HSP 1 Score: 187 bits (474), Expect = 1.570e-54
Identity = 103/129 (79.84%), Postives = 104/129 (80.62%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD 388
            ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTID VK KIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV+
Sbjct:    6 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDNVKTKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVE 112
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BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: A0A7S4B1G7_CHRCT (Hypothetical protein (Fragment) n=2 Tax=Eukaryota TaxID=2759 RepID=A0A7S4B1G7_CHRCT)

HSP 1 Score: 186 bits (472), Expect = 1.940e-54
Identity = 103/129 (79.84%), Postives = 104/129 (80.62%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD 388
            ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTID VK KIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV+
Sbjct:   57 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDTVKTKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVE 163
[Close]
BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: A0A7S2ESJ4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S2ESJ4_9STRA)

HSP 1 Score: 187 bits (474), Expect = 2.230e-54
Identity = 103/129 (79.84%), Postives = 104/129 (80.62%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD 388
            ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTID VK KIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV+
Sbjct:   22 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDNVKTKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVE 128
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BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: H0Z8Y5_TAEGU (Uncharacterized protein n=3 Tax=Taeniopygia guttata TaxID=59729 RepID=H0Z8Y5_TAEGU)

HSP 1 Score: 193 bits (491), Expect = 3.060e-54
Identity = 115/189 (60.85%), Postives = 123/189 (65.08%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD-----------------------------SWRTAAPFRTTTSKRSRPSTWCCACAAGNRF 481
            ESTLHLVLRLRGGMQIFVKTLTGKTITL+VEPSDTI+ VK KIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKEST HLVLRLRGGMQIFVKTLTGKTITL+V+                             SW+  AP  TTTS+RS+  TWCCA     +F
Sbjct:  216 ESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKEST-HLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGSWKMGAPCPTTTSRRSQLCTWCCASGVAVKF 381
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BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: UBIQP_PHYIN (Polyubiquitin n=16 Tax=Eukaryota TaxID=2759 RepID=UBIQP_PHYIN)

HSP 1 Score: 187 bits (476), Expect = 3.430e-54
Identity = 103/129 (79.84%), Postives = 105/129 (81.40%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD 388
            ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSD+ID VKQKIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV+
Sbjct:   64 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDSIDNVKQKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVE 170
[Close]
BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: A0A7R9U6E0_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U6E0_9STRA)

HSP 1 Score: 185 bits (470), Expect = 4.990e-54
Identity = 102/129 (79.07%), Postives = 104/129 (80.62%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVD 388
            ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTI+ VK KIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV+
Sbjct:   34 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIENVKTKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVE 140
[Close]
BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Match: A0A7S3XFR2_9CHLO (Hypothetical protein n=1 Tax=Picocystis salinarum TaxID=88271 RepID=A0A7S3XFR2_9CHLO)

HSP 1 Score: 185 bits (470), Expect = 5.150e-54
Identity = 102/128 (79.69%), Postives = 104/128 (81.25%), Query Frame = 2 
Query:    2 ESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSRTRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV 385
            ESTLHLVLRLRGGMQIFVKTLTGKTITL+VEPSD+ID VKQKIQDKEGIPPDQQRLIFAGK                       LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV
Sbjct:   15 ESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDSIDNVKQKIQDKEGIPPDQQRLIFAGKQ----------------------LEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDV 120
The following BLAST results are available for this feature:
BLAST of mRNA_P-littoralis_Contig130.61.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5K2A1_9PHAE1.140e-5581.40Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6S8BTP7_9STRA1.610e-5579.84Hypothetical protein (Fragment) n=1 Tax=Aureoumbra... [more]
A0A7S1UDB6_9STRA5.820e-5580.62Hypothetical protein (Fragment) n=1 Tax=Phaeomonas... [more]
A0A7S4VZT1_9STRA1.570e-5479.84Hypothetical protein (Fragment) n=3 Tax=Ditylum br... [more]
A0A7S4B1G7_CHRCT1.940e-5479.84Hypothetical protein (Fragment) n=2 Tax=Eukaryota ... [more]
A0A7S2ESJ4_9STRA2.230e-5479.84Hypothetical protein (Fragment) n=1 Tax=Ditylum br... [more]
H0Z8Y5_TAEGU3.060e-5460.85Uncharacterized protein n=3 Tax=Taeniopygia guttat... [more]
UBIQP_PHYIN3.430e-5479.84Polyubiquitin n=16 Tax=Eukaryota TaxID=2759 RepID=... [more]
A0A7R9U6E0_9STRA4.990e-5479.07Hypothetical protein (Fragment) n=1 Tax=Pinguiococ... [more]
A0A7S3XFR2_9CHLO5.150e-5479.69Hypothetical protein n=1 Tax=Picocystis salinarum ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-littoralis_Contig130contigP-littoralis_Contig130:277539..279165 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Pylaiella littoralis U1_48 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog6211.A0A068Y074
Preferred nameUBB
PFAMsubiquitin
Model size1146
Max annot lvl33208|Metazoa
KEGG koko:K04551,ko:K08770
KEGG Pathwayko03320,ko04137,ko04144,ko05012,ko05167,map03320,map04137,map04144,map05012,map05167
Hectar predicted targeting categoryother localisation
Exons4
Evalue2.53e-57
EggNOG OGsCOG5272@1|root,KOG0001@2759|Eukaryota,38ECC@33154|Opisthokonta,3B9NC@33208|Metazoa,3CXIG@33213|Bilateria
DescriptionUbiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
Cds size495
COG categoryO
BRITEko00000,ko00001,ko04121,ko04147
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-littoralis_Contig130.61.1prot_P-littoralis_Contig130.61.1Pylaiella littoralis U1_48polypeptideP-littoralis_Contig130 278150..279125 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789590.7793229-UTR-P-littoralis_Contig130:277538..2781491680789590.7793229-UTR-P-littoralis_Contig130:277538..278149Pylaiella littoralis U1_48UTRP-littoralis_Contig130 277539..278149 -
1680789590.841899-UTR-P-littoralis_Contig130:279125..2791651680789590.841899-UTR-P-littoralis_Contig130:279125..279165Pylaiella littoralis U1_48UTRP-littoralis_Contig130 279126..279165 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789590.7999015-CDS-P-littoralis_Contig130:278149..2782971680789590.7999015-CDS-P-littoralis_Contig130:278149..278297Pylaiella littoralis U1_48CDSP-littoralis_Contig130 278150..278297 -
1680789590.8089533-CDS-P-littoralis_Contig130:278388..2785221680789590.8089533-CDS-P-littoralis_Contig130:278388..278522Pylaiella littoralis U1_48CDSP-littoralis_Contig130 278389..278522 -
1680789590.8204894-CDS-P-littoralis_Contig130:278740..2788101680789590.8204894-CDS-P-littoralis_Contig130:278740..278810Pylaiella littoralis U1_48CDSP-littoralis_Contig130 278741..278810 -
1680789590.8329551-CDS-P-littoralis_Contig130:278982..2791251680789590.8329551-CDS-P-littoralis_Contig130:278982..279125Pylaiella littoralis U1_48CDSP-littoralis_Contig130 278983..279125 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-littoralis_Contig130.61.1

>prot_P-littoralis_Contig130.61.1 ID=prot_P-littoralis_Contig130.61.1|Name=mRNA_P-littoralis_Contig130.61.1|organism=Pylaiella littoralis U1_48|type=polypeptide|length=165bp
MQIFVKTLTGKTITLDVEPSDTIDGVKQKIQDKEGIPPDQQRLIFAGKSR
TRRASLRTSSASSSPASSWRTLEDGRTLSDYNIQKESTLHLVLRLRGGMQ
IFVKTLTGKTITLDVDSWRTAAPFRTTTSKRSRPSTWCCACAAGNRFGQY
LSVAAPFLSICVPL*
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mRNA from alignment at P-littoralis_Contig130:277539..279165-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-littoralis_Contig130.61.1 ID=mRNA_P-littoralis_Contig130.61.1|Name=mRNA_P-littoralis_Contig130.61.1|organism=Pylaiella littoralis U1_48|type=mRNA|length=1627bp|location=Sequence derived from alignment at P-littoralis_Contig130:277539..279165- (Pylaiella littoralis U1_48)
GGAGTCGACCCTCCACCTGGTGCTGCGGCTGCGTGGTGGCATGCAGATTT
TTGTCAAGACCCTCACGGGCAAGACCATCACTCTGGATGTGGAGCCGTCG
GACACGATCGACGGCGTGAAGCAAAAGATCCAGGACAAGGAGGGCATCCC
TCCGGACCAGCAGCGCCTCATCTTCGCCGGCAAGCAGCTGGAGGACGGCC
GCACCCTGTCGGACTACAACATCCAGAAGGAGTCGACCCTCCACCTGGTG
CTGCGCCTGCGCGGTGGCATGCAGATCTTTGTCAAGACCCTCACGGGCAA
GACCATCACCCTTGATGTGGAGCCGTCGGACACGATCGACGGCGTGAAGC
AGAAGATCCAGGACAAGGAGGGCATCCCTCCGGACCAGCAGCGCCTCATC
TTCGCCGGCAAGCAGCTGGAGGACGGCCGCACCCTGTCGGACTACAACAT
CCAGAAGGAGTCGACCCTCCACCTGGTGCTTCGCCTGCGTGGTGGCATGC
AGATCTTTGTCAAGACCCTCACGGGCAAGACCATCACTCTGGATGTGGAG
CCGTCGGACACGATTGACGGCGTGAAGCAAAAGATCCAGGACAAGGAGGG
CATCCCTCCGGACCAGCAGCGCCTCATCTTCGCCGGCAAGCAGCTGGAGG
ACGGTCGCACCCTGTCGGACTACAACATCCAAAAGGAGTCGACCCTCCAC
CTGGTGCTGCGCCTGCGTGGTGGCATGCAGATTTTTGTCAAGACCCTCAC
GGGCAAGACCATCACCCTGGATGTGGAGCCGTCAGACACGATCGACGGCG
TGAAGCAAAAGATCCAGGACAAGGAGGGCATCCCCCCCGACCAGCAGCGC
CTCATCTTCGCCGGCAAGCAGCTGGAGGACGGCCGCACCCTTTCGGACTA
CAACATCCAAAAGGAGTCGACCCTCCACCTGGTGCTGCGCCTGCGCGGCG
GGCAATAGATTTGGCCAATATTTGTCGGTTGCCGCTCCTTTTCTTTCAAT
TTGTGTCCCGCTGTAAGTAGTGGTTGTCGAAAAGTCTGAGGGGCCGTCTT
CCGTGTTTGTTTTTATTGGTACTGTTGCCTGCAATAGCCGACACTGGCGT
GACTTCTTCTGTGAACGAGGGGTTATATGGGTCGTGGATGGGAATTTGCC
CCGCTTGTGGTTTCTCTCACTTGCACTCCTGTCGGGATGCAACCCTTGTC
TGACTCTGAGTCGGCCATCTGTTGTATACAGGCTCGACCTTAGATTGTAG
AGGAGCACGATTGTTCTGTCTGTGGTAGTGTCTCTCTTCCGTGTACTGGT
GCCCAGAGGCTTTTGATTCAAAATGACGCAATCTGAGGTCAAGCAGGGCA
GATTTCATGTTCTCCAAGGGTCATGCTTTCATGGTCCTGATACTTGTTGT
CGTTGCCAGCACCAGGAGGAGTAAATGTGGCATGTGTGAAAACCAGTGTA
ACATGTCAAAATCTCAAAAGCCTCATTGCTCTGTACTTCGTCATGCTTGT
TGTGGACAACATGGCGTCCCGTTTCCGCTCTGCACCGAACTTCGCACGTG
ATGCGAATAGCTGGCCGTTTTTGGACGGCATCAGTTCAGTCGGCTTTGGG
AATCAAACTTTCACCAAAAACTGTCCT
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Coding sequence (CDS) from alignment at P-littoralis_Contig130:277539..279165-

>mRNA_P-littoralis_Contig130.61.1 ID=mRNA_P-littoralis_Contig130.61.1|Name=mRNA_P-littoralis_Contig130.61.1|organism=Pylaiella littoralis U1_48|type=CDS|length=495bp|location=Sequence derived from alignment at P-littoralis_Contig130:277539..279165- (Pylaiella littoralis U1_48)
ATGCAGATTTTTGTCAAGACCCTCACGGGCAAGACCATCACTCTGGATGT
GGAGCCGTCGGACACGATCGACGGCGTGAAGCAAAAGATCCAGGACAAGG
AGGGCATCCCTCCGGACCAGCAGCGCCTCATCTTCGCCGGCAAATCCAGG
ACAAGGAGGGCATCCCTCCGGACCAGCAGCGCCTCATCTTCGCCGGCAAG
CAGCTGGAGGACGCTGGAGGACGGTCGCACCCTGTCGGACTACAACATCC
AAAAGGAGTCGACCCTCCACCTGGTGCTGCGCCTGCGTGGTGGCATGCAG
ATTTTTGTCAAGACCCTCACGGGCAAGACCATCACCCTGGATGTGGACAG
CTGGAGGACGGCCGCACCCTTTCGGACTACAACATCCAAAAGGAGTCGAC
CCTCCACCTGGTGCTGCGCCTGCGCGGCGGGCAATAGATTTGGCCAATAT
TTGTCGGTTGCCGCTCCTTTTCTTTCAATTTGTGTCCCGCTGTAA
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