mRNA_P-littoralis_Contig910.14.1 (mRNA) Pylaiella littoralis U1_48

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: D7FVS7_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FVS7_ECTSI)

HSP 1 Score: 674 bits (1738), Expect = 8.160e-239
Identity = 366/501 (73.05%), Postives = 397/501 (79.24%), Query Frame = 2

Query:  215 RRRQRPTSHQQSWTSRRTTAVLLYIFLGRCQGFILSNKLHHASAAAAAAISGHNIHRATGLDNLASPHRHDHHRGVSKPVLLR-QYQQRYPQFFSRPRKTVTTMAAAPRNGG-YGRFSDTDDEPAYRPFPPAAAAAGARARKPGRDWQEIGNADVLVPKDVDYPLGVVHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATRVRLNEFNHDALACDVARDFRVAYRDVEALYGRAAMARIPLFGVGHSLGAKVHVLLNCYPEVVDVARRRKANVIISFNNFPASESVPFINELRELGLSMGDLGSLGDNLSGIAKAVAGIPLMSGFAMQ----AEEWAPKAAAAVGSALSDVPQEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGRDGELEFSRRVFCAGKRSQALDGTHVTPNTPDFRDTRQARDWANKAGMGAEGGEFVEARARAVASTARLELDELIKTLSSYLRSQSLWVRREGRWDNDEM 1699
            RR++RP   QQ W S  +  VLL + LGRCQGF  S+K HH                 T LD+ A+ H  +H R V+ PVL R + ++R+P           T+A A R+ G YGRFSDT DEPAYRP    A AAG  +R  GRDW+EIGNADVLVP+DVDYPLGVVHFVGGQGVGVFPRN YGALLEGLVDAGFLVVATRVRLNEFNHD LACDVARDFRVAYRDVEALYGR AM RIPLFGVGHSLGAKVHVLLNCYPEVVDVARRRKANVIISFNNFPA +SVPFINELRELGLSM + G LGDNLSGIAKAV+G+PL++GFA Q    AEEW PKAA+AVGS LSDVPQEF PSP+ TWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAE LFNRFGRDGELEFSR           LDGTHVTPNTPDFRDTRQARDWA++AGMGA+G  FVE  AR  ASTA LELDELIK L+SYLRSQSLWVRREGRWD+DEM
Sbjct:    7 RRQRRPV--QQPWASGTSAIVLLALLLGRCQGFAFSSKAHH-----------------TRLDSPAA-HNSNHRRDVAAPVLQRSRRRRRHP--------ASPTIAGAHRDSGDYGRFSDTGDEPAYRP----ATAAGGASRS-GRDWEEIGNADVLVPRDVDYPLGVVHFVGGQGVGVFPRNAYGALLEGLVDAGFLVVATRVRLNEFNHDQLACDVARDFRVAYRDVEALYGRTAMRRIPLFGVGHSLGAKVHVLLNCYPEVVDVARRRKANVIISFNNFPAKDSVPFINELRELGLSMENFGPLGDNLSGIAKAVSGLPLVNGFASQGLSLAEEWGPKAASAVGSMLSDVPQEFKPSPEATWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAEVLFNRFGRDGELEFSR-----------LDGTHVTPNTPDFRDTRQARDWADRAGMGAQGDAFVEGGARVAASTASLELDELIKALTSYLRSQSLWVRREGRWDSDEM 463          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: A0A836CAD4_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CAD4_9STRA)

HSP 1 Score: 336 bits (861), Expect = 1.390e-106
Identity = 181/353 (51.27%), Postives = 232/353 (65.72%), Query Frame = 2

Query:  647 WQEIGNADVLVPKDVDYPLGVVHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATRVRLNEFNHDALACDVARDFRVAYRDVEALYGRAAMARIPLFGVGHSLGAKVHVLLNCYPEVVDVARRRKA---NVIISFNNFPASESVPFINELRELGLSMGDLGSLGDNLSGIAKAVAGIPLMSGFAMQAE-------EWAPKAAAAVGSALSDVPQEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGRDGELEFSRRVFCAGKRSQALDGTHVTPNTPDFRDTRQARDWANKAGMG----AEGGEFVEARARAVASTARLELDELIKTLSSYLRSQSLW 1663
            W+ +G  +VL+PK V++PLGVVHF+GGQ VG  PR TYG  LEG+  AGF+V+AT +R+NEFNH  LACDVAR+FR+ YRDVEALYGRAA+ R P+FG+GHS+GAK+ VL+NC+P+V DVARRRKA   NV+ISFNNF A +SVP + E+R LGL    LG L + +  + + VA + L  G +  AE       EW        G+ L  VP EF P+P   W LL   Y+VRNNLV+QFK DT+DQS +L+ T+F+RFGRDG L++S            L G+HVTPNTPD  +     +W   A  G    A       +  RA AS A  ELDELI+T+  YL+SQ+LW
Sbjct:   95 WKRVGKVEVLLPKGVEFPLGVVHFIGGQAVGAVPRATYGPFLEGIARAGFVVLATPIRINEFNHQELACDVAREFRLCYRDVEALYGRAALRRAPVFGIGHSMGAKLQVLINCFPDVRDVARRRKAIEANVLISFNNFAARDSVPLLAEMRALGLR---LGGLSEGVKAVRETVASLGL-GGLSPMAERGLNLAVEW--------GAGLGGVPDEFKPAPDELWQLLEGEYAVRNNLVVQFKSDTLDQSGRLSRTIFDRFGRDGALQYS-----------TLQGSHVTPNTPDLSEV-DLDEWLTAAAGGGPAAARAAGVASSGVRASASVAAAELDELIRTVVIYLKSQALW 423          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: A0A7S2UUU1_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UUU1_9STRA)

HSP 1 Score: 191 bits (484), Expect = 1.170e-52
Identity = 125/319 (39.18%), Postives = 172/319 (53.92%), Query Frame = 2

Query:  788 AGFLVVATRVRLNEFNHDALACDVARDFRVAYRDVEALYGRAAMARIPLFGVGHSLGAKVHVLLNCYPEV--VDVARRRKANVIISFNNFPASESVPFINELRELGLSMGDLGSLGDNLSGIAKAVAG-----IP-----------LMSGFAMQAEEWAPKAAAAVGSALSDVPQEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGRDGELEFSRRVFCAGKRSQALDGTHVTPNTPDFRDT--RQARDWANKAGMGAEGGEFVEARARAVASTARLELDELIKTLSSYLRSQSLWVRREGRW 1684
            AGFLVVAT +  N F+H A A +  R FR A+RD+E LYG A   R+P FG+GHSLGAK+ VL +C   V   D   RR+ANV+ISFNNF A ESVP + E+  LG  +G    L D  SG    VAG     +P           L  GF    E+ AP+ A  V   +S++  EF+P P+  W  + D+Y V+ NL++QFK+D+IDQSP L++ L  +  R G LEF R           LDG H+TP   D ++   +   DW           +F +       S A +E +E++  + ++LR  S+W +RE  W
Sbjct:    1 AGFLVVATPIMANTFDHRAEAMESCRQFRSAWRDIEDLYGPAQANRLPQFGLGHSLGAKLIVLSSCLRGVGEKDPIERRRANVLISFNNFSARESVPLLEEIGALGKDLGQFQPLLDQFSGQVSNVAGKVQTQLPRIEKSLFSTSFLKQGFEA-VEKVAPQIAGIVSDTVSNIEFEFSPDPEEMWQAVEDQYPVKKNLIIQFKKDSIDQSPYLSQLLDEKLDRQGTLEFIR-----------LDGNHLTPVMQDIQNDWFKAQPDWTKS--------KFTDE----FQSLAMMEFEEMLGIVINFLRENSVW-QREQTW 294          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: W7TQQ9_9STRA (Uncharacterized protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TQQ9_9STRA)

HSP 1 Score: 177 bits (448), Expect = 2.450e-45
Identity = 143/388 (36.86%), Postives = 187/388 (48.20%), Query Frame = 2

Query:  638 GRDWQEIGNA-DVLVPKDVDYPLGVVHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATRVRLNEFNHDALACDVARDFRVAYRDVEALYGRAAMARIPLFGVGHSLGAKVHVLLNCYPEVVD--------VARRRKANVIISFNNFPASESVPFINELRELGLSMGDL---GSLGD-----------NLSGIAKAVAGIPLMSGFAMQAEEWAPKAAAAVGSALSDVPQ----EFNPSPQTTWDLLTD--------------RYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGRDGELEFSRRVFCAGKRSQALDGTHVTPNTP------DFRDTRQA------------RDWANKAG---MGAEGGEFVEARARAVASTARLELD 1615
            G +WQ +    DV  P+    P G+VHFVGG  VG FPR  YG LLE L   G+ V+AT +  + F+H A A  +A  +      VE +YG     ++P+FG+GHSLGAK+ +LL   P  +          A  R+ANV+++FNNF A +SVP + ELRELG S+  +   G L D           NLSG +   A     SGF     + A K        LS +P     +F PSP+ TW LL+                Y+V NNLV+ F +DTIDQS +L   L  R GR+G     R           L GTH+TPNTP      D  +  +             R W  + G   +GAEGG   +A AR VA  AR ELD
Sbjct:  131 GGEWQRVDEGVDVFFPEASAPPKGLVHFVGGAVVGAFPRAAYGPLLERLGRQGYCVIATALPFSLFDHAAGARALAMAYARGEAYVEGVYGPRVRQQLPVFGLGHSLGAKMLILLGSPPPSLPSFSSSSAPTAGPRRANVLVAFNNFSAKQSVPLLEELRELGQSVAGMDLSGKLEDISRMVSSLAALNLSGASSPNAQRETTSGF-----DLASKGLTMAMDFLSSLPTSGVPDFTPSPEETWALLSSPPLFPSSSLPSSSPSYAVLNNLVVSFDKDTIDQSEELVALLRER-GREGGRGGGRVEHVR------LVGTHLTPNTPRGVLDADSEEKMEEVKEKDRVWDTYERKWVEEVGEERLGAEGGS--DASARTVAKVARRELD 504          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: A0A8J6CFW4_DIALT (Uncharacterized protein n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J6CFW4_DIALT)

HSP 1 Score: 159 bits (403), Expect = 1.470e-39
Identity = 142/380 (37.37%), Postives = 187/380 (49.21%), Query Frame = 2

Query:  599 PAAAAAGARARKPGRD---WQEIGNADVLVPKDVDYPLGVVHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATR-VRLNEFNHDALA---CDVARDFRVAYRDVEALYGRAAMARIPLFGVGHSLGAKVHVLLNCYPEVVD-VARRRKANVIISFNNFPASESVPFINELR---ELGLSMGDLGS-----LGDNLSG-IAKAVAGIPLMSGFAMQAEEWAPKAAAAVGSALSDVP--QEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRF-----GRDGELEFSRRVFCAGKRSQALDGTHVTPNTPDFRDTRQARDWANKAGMGAEGGEFVEARARAVASTARLELDELIKTLSSYLRSQS-LW 1663
            P A AAGARA  P  +   W+ I   DVL+P D     GVVHFVGG  VGV PR  YG LLE L   G+ ++AT   +L  F+H A A   C+   D   A R   A+  R    R+P+FG+GHSLGAKV VL          +  RR AN ++S+NN+ A  SVP    L     +GL     G+     L   L G I +  AG  + + F     E   + +  +G+AL+ V    +F P P+ T + +   Y V +NL+++F  DTI +S  LA  L  RF     G  G L + R           L GTHVTPNTPD R           AG+GA+ G    A A+A+   A  ELD LI TL  +   ++ LW
Sbjct:   64 PRARAAGARAPAPEDEPAPWERIEGCDVLLPPDAVDVRGVVHFVGGALVGVVPRQAYGPLLESLAATGYAIIATPDPQLLGFDHGAAARAVCERYADAAFAIRQRYAVDDR----RVPVFGLGHSLGAKVLVLAGSDGACARALGVRRAANALLSYNNYSARRSVPLFEPLLAALRVGLDGAGAGAASARELVRQLEGAIDEYAAGTEIAAPFRAGLRELG-RLSGQLGTALAGVALADDFTPGPEETLERVRRAYDVSSNLLVRFSADTICESEALARELRARFTAPDTGIGGRLSYKR-----------LRGTHVTPNTPDLR-----------AGVGADAG---PAAAQAL-DAAVAELDALIDTLLIFFDKEARLW 412          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: A0A7S1XE42_9RHOD (Hypothetical protein n=1 Tax=Compsopogon caeruleus TaxID=31354 RepID=A0A7S1XE42_9RHOD)

HSP 1 Score: 149 bits (375), Expect = 7.170e-36
Identity = 115/308 (37.34%), Postives = 161/308 (52.27%), Query Frame = 2

Query:  638 GRDWQEIGNADVLVPKDVDYPLGV-------VHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATRVRLNEFNHDALACDVARDFRVAYRDVEALYGRAA--MARIPLFGVGHSLGAKVHVLLNCYPEVV-DVARRRKANVIISFNNFPASESVPFINEL---------RELGLSMGDLGSLGDNLSGIAKAVAGIPLMSGFAMQAEEWAPKAAAAVGSALSDVP-QEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGRDGELEFSRRVFCAGKRSQALDGTHVTPNTPDFR 1501
            G  W E+   DV++P D+  P G        VHF+GG G+G  P+  Y  L+E L + G +VVAT V L +FNH  LA D A  F      +E+   R +  +  +P+ GVGHSLGAK+HVLLN   +V  D    R  NV+IS+NNF A +S+P  N++         R  G+  G +GS+ D +  IA ++ GIP  S  ++ A          + +AL  +  +EF P P    D+L ++Y+V NN V+QF  D+ID S  LA  L  RF        SR         + L GTH+TP TPD +
Sbjct:   65 GLSWSEVNGCDVVMPVDLGEPSGGAARRPVWVHFMGGAGLGSRPKQFYRPLMERLAEQGCIVVATPVGL-QFNHTQLARDSAITFMETRAAIESSKVRESSRIGFLPVLGVGHSLGAKLHVLLNSDEDVRRDYVGARIGNVLISYNNFSALQSIPRWNDMIGGMGAASERIRGVRPG-VGSILDGVRSIANSL-GIPERSLDSLNAVG---PFLDGLNTALDSLQVEEFLPPPAKVDDMLQNQYAVANNCVVQFVEDSIDDSDNLARVLTTRFS-------SRNTVF-----RRLSGTHLTPVTPDLQ 354          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: U9VHV5_9CYAN (Uncharacterized protein n=1 Tax=Leptolyngbya sp. Heron Island J TaxID=1385935 RepID=U9VHV5_9CYAN)

HSP 1 Score: 139 bits (349), Expect = 7.460e-34
Identity = 94/288 (32.64%), Postives = 137/288 (47.57%), Query Frame = 2

Query:  647 WQEIGNADVLVPKDVDYPLGVVHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATRVRLNEFNHDALACDVARDFRVAYRDVEALYGRAAMAR---IPLFGVGHSLGAKVHVLLNCYPEVVDVARRRKANVIISFNNFPASESVPFINELRELGLSMGDLGSLGDNLSGIAKAVAGIPLMSGFAMQAEEWAPKAAAAVGSALSDVPQEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGRDGELEFSRRVFCAGKRSQALDGTHVTPNTPDFR 1501
            WQE+    +LVP   D P  ++HF+GG  +   P  TYG LLE L   G+++VAT   +N F+HDA+A +V   F     D   +Y R  +     +P++G+GHS+G KVH+L+     V      R  N++ISFNN+PA +S+P + +  +                                     +AP           D   EF PSP  T DL+ DRY+V  NL+++F++DTIDQ+  L++ LF RF +   +             Q L GTH TP   D R
Sbjct:    3 WQEVSGNWLLVP---DRPRAIIHFLGGAFIAAAPNLTYGWLLENLSQQGYIIVATPF-INTFDHDAIAAEVLTTF-----DQAMIYLRRQVIGDRYLPIYGMGHSMGCKVHLLIASLYNV-----DRAGNILISFNNYPARKSIPLLEQFVQ-------------------------------------FAP-----------DFDIEFTPSPGQTLDLVRDRYTVGRNLLIKFRKDTIDQTYDLSDVLFQRFPQFTAI-------------QILQGTHTTPVAQDVR 215          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: A0A0P4UIV2_9CYAN (Alpha/beta superfamily hydrolase n=3 Tax=Pseudanabaenales TaxID=2881377 RepID=A0A0P4UIV2_9CYAN)

HSP 1 Score: 137 bits (345), Expect = 3.000e-33
Identity = 94/286 (32.87%), Postives = 137/286 (47.90%), Query Frame = 2

Query:  644 DWQEIGNADVLVPKDVDYPLGVVHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATRVRLNEFNHDALACDVARDFRVAYRDVEALYGRAAMARIPLFGVGHSLGAKVHVLLNCYPEVVDVARRRKANVIISFNNFPASESVPFINELRELGLSMGDLGSLGDNLSGIAKAVAGIPLMSGFAMQAEEWAPKAAAAVGSALSDVPQEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGRDGELEFSRRVFCAGKRSQALDGTHVTPNTPDFR 1501
            DWQEI    VLVP   D P+ ++HF+GG  V   P+ TY  LLE L D G+L+VAT   +N F+H  +A  V   F  A R + +   + ++  +P++G+GHS+G K+H+L+     V      R  N+ ISFNN+ A E+VPF+ +  +     G                        F++                      EF PSP  T +L+ DRYSVR NL+++F  D+IDQS  LA+ L +             ++     +  L GTHVTP  PD +
Sbjct:    2 DWQEISGNWVLVP---DRPIAIIHFLGGAFVATAPQLTYRRLLEFLGDQGYLIVATPF-VNTFDHIEIAKSVLMSFDRALRGIRS---QLSVQYLPIYGMGHSMGCKLHLLIGSLFAV-----ERAGNIFISFNNYAAKEAVPFVEQFSQFLKPQG------------------------FSV----------------------EFTPSPSETKELIRDRYSVRRNLIIKFADDSIDQSLPLAQLLED-------------IYPGMITTHRLKGTHVTPLGPDMK 216          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: Q31PG7_SYNE7 (Uncharacterized protein n=5 Tax=Synechococcus elongatus TaxID=32046 RepID=Q31PG7_SYNE7)

HSP 1 Score: 137 bits (345), Expect = 3.500e-33
Identity = 90/255 (35.29%), Postives = 128/255 (50.20%), Query Frame = 2

Query:  647 WQEIGNADVLVPKDVDYPLGVVHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATRVRLNEFNHDALACDVARDFRVAYRDVEALYGRAAMARIPLFGVGHSLGAKVHVLLNCYPEVVDVARRRKANVIISFNNFPASESVPFINELRELGLSMGDLGSLGDNLSGIAKAVAGIPLMSGFAMQAEEWAPKAAAAVGSALSDVPQEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGRDGE 1411
            WQ I N  VL+P+    P  +VHF+GG  +   P+ TY  LLEGL D GF V+AT   +N F+H  LA +V R +R+AY  + AL        +P+FGVGHS+G+K+H+L+ C  +  D    R+ NV+++FNN+ A  S+P + +L                           P +                       DV  EF+PSP  T  L+ DRYSV NNL+++F+ D IDQ+  L   L +RF    E
Sbjct:    6 WQTIYNNAVLLPES---PKAIVHFLGGAFIAAAPQVTYRRLLEGLGDRGFAVIATPF-INTFDHTTLADEVGRSYRLAYERLLAL--GLISPELPIFGVGHSMGSKLHLLMGC-AQTSD----RRGNVLLAFNNYSARRSIPLLGQLA--------------------------PAL-----------------------DVTVEFSPSPARTLRLIRDRYSVANNLLVKFRNDEIDQTRDLIGALRDRFPETSE 200          

BLAST of mRNA_P-littoralis_Contig910.14.1 vs. uniprot
Match: A0A7S0Q4U5_9EUKA (Hypothetical protein n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0Q4U5_9EUKA)

HSP 1 Score: 140 bits (354), Expect = 5.530e-33
Identity = 112/328 (34.15%), Postives = 160/328 (48.78%), Query Frame = 2

Query:  602 AAAAAGARARKPGRDWQEIGNADVLVPKDVDYPLGVVHFVGGQGVGVFPRNTYGALLEGLVDAGFLVVATRVRL-NEFNHDALACDVARDFRVAYRDVEALYGRAA--MAR-IPLFGVGHSLGAKVHVLLNCYPEVV--DVARRRKANVIISFNNFPASESVPFINELRELGLSMGDLGSLGDNLSGIAKAVAG----IPL-----------------MSGFAMQAEEWAPKAAAAVGSALSDVPQEFNPSPQTTWDLLTDRYSVRNNLVLQFKRDTIDQSPQLAETLFNRFGR-DGELEFSRRVFCAGKRSQALDGTHVTPNTPDFR 1501
            A AA+ +    P   W ++   DVL+P   +  + VVHFVGG  VG  P+  Y + LE LV  G  VVAT   L ++ +H A A + A  +R A  +V  L    A  + R +P+FGV HSLGAK+ ++L   P+    D    R ANV++ FNNFPAS SVP + +   LG S     +  + L G+++ VA     +PL                 + G   +  E + +   + G+  ++   EF P+P  T  L+   Y +R NL+L+F  D IDQS +L   L  RF    G  EF           +   GTH+TPN PD R
Sbjct:   50 AGAASSSPWPPPQPCWCQVRGCDVLLPPPEEECVAVVHFVGGAFVGAAPQQAYRSFLELLVSFGLAVVATPCNLLSDLDHYAAATEAAARWRAAEPEVRELLRPWACELGRPLPVFGVAHSLGAKLLLILGSLPDGGGDDGVGPRVANVMLGFNNFPASRSVPLLEQAASLGSSAAP--ATAEALDGLSRLVADAACRLPLEASASPLDALGVAVGDLLDGAGGKLSELS-REVESFGADSAERAAEFRPTPAETEGLVLASYRIRRNLLLRFDADEIDQSVRLRSLLRRRFADIGGRTEF-----------RTTPGTHITPNLPDLR 363