mRNA_P-littoralis_Contig83.71.4 (mRNA) Pylaiella littoralis U1_48

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: D8LK25_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LK25_ECTSI)

HSP 1 Score: 1028 bits (2657), Expect = 0.000e+0
Identity = 617/1072 (57.56%), Postives = 693/1072 (64.65%), Query Frame = 2

Query:  665 RRYFGDDEDAFRSAVDWHHSRLRAAAGGDVELRGVEGGXXXXXXXXXE----VGAGAGARGTFAVFTSHRRGGRAREVLSAALSPSSVMTVAHNREQGGCFFVHASAADVDALLLLPEADXXXXXXXXXXXXXXXXXXXXRLFDGFVAVPPSLKLAPSLLDLDDD------------------------------------------------------GISPGKALRRNSGVEGLVVVLLSPPLASSADDEDGLALVERWRRDWSSASLDLHSLLFWSAEDGH-PRHCSGEREGEEDXXXXXXXXXXXXXAAAEVLAREWGGAARTVHALAERLGGVSPAEACGWNDVRVEAEGTGVGTRRRSSSSGWVTLRNIGHLLPG-ESPDKSQPTTRPGSSXXXXXXXXXXXXXXXXDS---------------------EREKKACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRDADGSLGTGDFQYDLSQRKVVQYIEGNMGSPDFIDDDEGGHGTHVAGSVAGSIYSGWTGPNACPDGVAGATADQTAISCVGACLAPSVLAEYVANNIFDLGALCPEYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDVGTSDLLEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLGDDISDCSISAPGLSKNSLAVGSSMSGGARLSPGEMDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGGDGSCELDKNSGTSMSCPIAAGAAALVRQYFVDGFYSDDLEARGGLCTATS--RWACDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDADGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGG-PGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTMSGMCSGDEASCSLDNRNNVERIVVPASDVTAGTWTVRVSAGNGLVEGGLQSYALAVSLPISSGGTAATTA 3628
            RR+FG DE+AFR AVDW HSRL A A            XXXXXXXX E      A  G  GTF  FTSH RG R RE LSAALSP+SV+T AHNREQGGCF V ASAADV++L+      XXXX                RLFDGFVAVPPSLKLAPSLLD   D                                                      G  PGKALR N+G EGLVV LL  P  + + +    AL + W+R WSS  LDL  L FWS  DGH PR    E+             XXXXX AA+ LAREWG AARTVHALAERLGGVSPAEACGWNDVRV AEG G     RSSS+GWVTLR+IGHLL G E  +++QP  +  SS    XXXXXXXXX    S                     EREK ACLMGLVSFLA+RPEVARVSALPRAEL N VA RIVQG SAT+ PIWDRGVDGSG+VVQVVDSG+ +GSCFFR+SATT GPDHGYLRD+DG+LG GD+ YDLSQRK                                                                                       Y+CD YW + CLSDDRSETLE AKGVAP AKLA+LD+G+SD LEQA+GGTMWE S G GAR+HS SWGF D+PCT+DD  VSFDEWA++NPEHLL+FAAGNLGDD+  CSI++P LSKN LAVGSSMSG  RLSPG++D+VSDFSS GPT D RIKPDI+APGH++FSA+NGG+GSC+LD+NSGTSMSCPIAAGAAALVRQY VDGFY+DD+ ARG LC A +   WACD FSPSGALVKA+LIHGADAMGGSTDPDG RGFGRI+L+SA+PFEGE +WALY+EDADG+TTSAGQ   V A E E R+F                  PGGIRATL WMDP AT SSSVQL+HDLDLF++GPDGTTWTMSG C+ D+ SC LD+RNNVER+VVPASD+TAGTWTV VSA   LVEGG Q+YALAVSLPIS     +T A
Sbjct:    5 RRFFGHDEEAFRLAVDWQHSRLLATAXXXXXXXXXXXXXXXXXXXXAEGEKEAVARRGGTGTFVAFTSHARGARGREALSAALSPTSVLTAAHNREQGGCFIVRASAADVESLMXXXXXXXXXXGPDGGISGS-------RLFDGFVAVPPSLKLAPSLLDYGSDFDAYGLGVGVDVSGGRDGGSGRGLAGGESSNAGAPGLAPAAATGAMASGERRGAGTLPGKALRHNTGGEGLVVQLLLSPETTGSRESALAALAQSWKRGWSSDLLDLGRLSFWSDADGHRPREAGDEQA-----------PXXXXXTAADFLAREWGSAARTVHALAERLGGVSPAEACGWNDVRVAAEGGG-----RSSSAGWVTLRDIGHLLHGAERDERAQPPRQARSSDTSGXXXXXXXXXSAGSSGGHPASGDGGMDDGSGSASASEREKTACLMGLVSFLAARPEVARVSALPRAELFNAVAGRIVQGGSATTQPIWDRGVDGSGEVVQVVDSGIDEGSCFFRDSATTLGPDHGYLRDSDGTLGAGDYTYDLSQRK---------------------------------------------------------------------------------------YDCDVYWETACLSDDRSETLEDAKGVAPGAKLAVLDLGSSDSLEQALGGTMWEVSAGTGARIHSASWGFIDEPCTVDDSAVSFDEWAYENPEHLLVFAAGNLGDDMEGCSIASPALSKNCLAVGSSMSGELRLSPGDLDEVSDFSSLGPTTDDRIKPDILAPGHYIFSASNGGEGSCQLDRNSGTSMSCPIAAGAAALVRQYLVDGFYADDVAARGSLCAAATDPAWACDAFSPSGALVKALLIHGADAMGGSTDPDGQRGFGRINLDSALPFEGEGVWALYMEDADGSTTSAGQ---VEAEETEVRTFTXXXXXXXXXXXXXXXXXPGGIRATLVWMDPPATTSSSVQLVHDLDLFVEGPDGTTWTMSGQCTSDQDSCILDDRNNVERVVVPASDITAGTWTVTVSASKDLVEGGPQTYALAVSLPISIAEAESTAA 963          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: A0A6H5JBB4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBB4_9PHAE)

HSP 1 Score: 936 bits (2420), Expect = 8.070e-312
Identity = 524/878 (59.68%), Postives = 587/878 (66.86%), Query Frame = 2

Query:  665 RRYFGDDEDAFRSAVDWHHSRLRAAAGGDVELR------GVEGGXXXXXXXXXEVGAGAGARGTFAVFTSHRRGGRAREVLSAALSPSSVMTVAHNREQGGCFFVHASAADVDALLLLPEADXXXXXXXXXXXXXXXXXXXXRLFDGFVAVPPSLKLAPSLLDLDDD---------------------------------------------------GISPGKALRRNSGVEGLVVVLLSPPLASSADDEDGLALVERWRRDWSSASLDLHSLLFWSAEDGH-PRHCSGEREGEEDXXXXXXXXXXXXXAAAEVLAREWGGAARTVHALAERLGGVSPAEACGWNDVRVEAEGTGVGTRRRSSSSGWVTLRNIGHLLPGESPDKSQPTTRP----------------------GSSXXXXXXXXXXXXXXXXD---SEREKKACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRDADGSLGTGDFQYDLSQRKVVQYIEGNMGSPDFIDDDEGGHGTHVAGSVAGSIYSGWTGPNACPDGVAGATADQTAISCVGACLAPSVLAEYVANNIFDLGALCPEYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDVGTSDLLEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLGDDISDCSISAPGLSKNSLAVGSSMSGGARLSPGEMDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGGDGSCELDKNSGTSMSCPIAAGAAALVRQYFVDGFYSDDLEARGGLCTATS--RWACDPFSPSGALVKAM 3043
            RR+FG DE+AFR AVDWHHSRL A               G   G         E    +G  GTF  FTSH R  R RE LSA LSP+SV+T AHNREQGGCF V AS ADV++LL      XXXXXXX             RLFDGFVAVPPSLKLAPSLLD   D                                                   G  PGKALRRN+G EGLVV LL  P A+ + +    AL E W+R WSS SLDL  L FWS  DGH PR    E+                   AA+ LAREWG AARTVHALAERLGGVSPAEACGWN VRV AEG G     RSSS+GWVTLR+IGHLLP    D   P  R                       GSS                    SEREK ACLMGLVSFLA+RPEV RVSALPRAEL N VA RIVQG SAT+ PIWDRGVDGSG+VVQVVDSG+ +GSCFFR+SATT GPDHGYLRD DG+LG GD+ YDLSQRKVVQYIE   G   + +DD  GHG+HVAGSVAGSIYSGWTGP  CPDGV  AT +QTA+SCVG CL P VL EY+++N FDL ALCPEY+CD YW + CLSDDRSETLE AKGVAP AKLA+LD+G+SD LEQA+GGTMWE S G GAR+HS SWGF D+PCT+DD +VSFD+WA++NPEHLL+FAAGNLGDD++ CSI++P +SKN LAVGSSMSG  RLSPG+MD+VSDFSS GPT D RIKPDI+APGH++FSA+NGGDGSC+LD+NSGTSMSCPIAAGAAALVRQY VDGFY++D+ ARG LC A +   WACD FSPSGALVK M
Sbjct:    5 RRFFGHDEEAFRLAVDWHHSRLLATXXXXXXXXXXXXXXGDNQGATGAAAQGEEAVGRSGGSGTFVAFTSHARAARGREALSAVLSPTSVLTAAHNREQGGCFIVRASTADVESLLXXXXXXXXXXXXXDGSSGGS------RLFDGFVAVPPSLKLAPSLLDYGSDFSSYDSGVGVLGGGRDGGRGLAGGDSSNAGPPGLAPDAATEAVASEERRGAGTLPGKALRRNTGGEGLVVQLLLSPEATGSRESALAALAESWKRSWSSDSLDLSRLSFWSDADGHRPREADDEQA------------PSPPRTAADFLAREWGSAARTVHALAERLGGVSPAEACGWNGVRVTAEGGG-----RSSSAGWVTLRDIGHLLPVAGRDDHAPPPRQVRXXXXXXXXXXXXXVNGNRSAGSSGGHPASGDRGMDDGSGSASASEREKTACLMGLVSFLAARPEVVRVSALPRAELFNAVAGRIVQGGSATTQPIWDRGVDGSGEVVQVVDSGIDEGSCFFRDSATTLGPDHGYLRDYDGTLGAGDYTYDLSQRKVVQYIESTRGDDVYSEDDPDGHGSHVAGSVAGSIYSGWTGPANCPDGVGDATEEQTAMSCVGVCLDPEVLEEYISDNTFDLDALCPEYDCDVYWEAACLSDDRSETLEDAKGVAPGAKLAVLDLGSSDSLEQALGGTMWEVSAGTGARIHSASWGFIDEPCTVDDSSVSFDQWAYENPEHLLVFAAGNLGDDMTGCSIASPAVSKNCLAVGSSMSGELRLSPGDMDEVSDFSSMGPTTDDRIKPDILAPGHYIFSASNGGDGSCQLDRNSGTSMSCPIAAGAAALVRQYLVDGFYAEDVGARGSLCAAGTDPAWACDAFSPSGALVKVM 859          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 463 bits (1191), Expect = 5.210e-136
Identity = 355/947 (37.49%), Postives = 474/947 (50.05%), Query Frame = 2

Query:  866 GGRAREVLSAALSPSSVMTVAHNREQGGCFFVHASAADVDALLLLPEADXXXXXXXXXXXXXXXXXXXXRLFDGFVAVPPSLKLAPSLLD-------LDDDGISPGK--------ALRRNSGVEGLVVVLLSPPLASSADDEDGLALVERWRRDWSSASLDLHSLLFWS--AEDGHPRHCSGEREGEEDXXXXXXXXXXXXXAAAEVLAREWGGAARTVHALAERLGGVSPAEACGWNDVRVEAEGTGVGTRRRSSSSGWVTLRNIGHLLPGESPDKSQPTTRPGSSXXXXXXXXXXXXXXXXDSEREKKACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRDADGSLGTGDFQYDLSQRKVVQYIEGNMGSPDFIDDDEGGHGTHVAGSVAGSIYSGWTGPNACPDGVAGATADQT-AISCVGACL-----APSVLAEYVANNIFDLGALCPEYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDV--GTSDLLEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLGD-DISDCSISAPGLSKNSLAVGSSMSGGARL----SPGEMDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGGDG--SCELDKNSGTSMSCPIAAGAAALVRQYFVDG-FYSDDLEARGGLCTATSRWACDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDADGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGGPGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTMSGMCSGDEASCSLDNRNNVERIVVPASDVTAGTWTVRVSAGNGLVEGGLQSYALAVSLPISSG 3607
            G  A   L   LSP +V  V+H+ + G CFF  AS A   A++   E                         + F   P  LKLAP L+D        +++  S G         A  R   VEGL V L    LA  A   +  + +     D  SAS+DLHS  FWS    DG   H S                      A  V AR+W  AA  VH L+E      P + C WN + V   G              + +  + HLL                                 +S     AC MGLVSFLA RPEV RVS  P   + N  A  ++Q ASAT TP+ D G+DG+G+++QV+DSG+ + SC+F + +     DHGY  D  G        Y   +   + +  G+     + +  + GHGTH AGS AG+  +         D     T + T  +SCVGAC+        +++     +  DL  LCP Y CD    + CLSDD  ETL    G+A  AKLAI DV  G  D    ++G  +WE    AG +LHS S G  D  C++    +  D++ ++N E+LLIFAAGN G  + S C+I+ PG+ KNSLA+G+S SG  R+        +D V++FSS G T D RIKP++VAPG  V+SAA+ G    SC L    GTSMSCPI AGAAA++RQYFVD  FY+ D+ ARG  C+    + C+ FSPS A VKA+LI+ A+ MGGS++PDG RGFGR+HLE+ MP EGE    L+V DA  T+ + G         +EE  F V          +A+ G   +RATL+W+DP ATA+S+VQL+H+LDL +  PDGTT+ M G       S + DNRN  ER++V A+DV  GTWTV V A N + +   QSY+L V+  IS G
Sbjct:   82 GHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFG---------------------LENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLA--ARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSP--------------------AGAVRARDWRKAASVVHELSEAAS-TGPGDICSWNRIFVHHAG-----------DDSLLVSGLDHLL---------------------FSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEE-VDHGYYFDELGRAAVYSSSYSSDETATI-FNGGDFSY--YPERRKAGHGTHTAGSAAGATLN---------DPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDA-SSSIGNNLWEVCTEAGCKLHSNSLG-ADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARG-FCS--DGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGT--------LEEYLFDV----------DADAGLD-LRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWG-------SDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDS--QSYSLVVNGAISPG 906          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 447 bits (1151), Expect = 1.950e-130
Identity = 382/1055 (36.21%), Postives = 506/1055 (47.96%), Query Frame = 2

Query:  629 LQRFHDVLETNSRRYFGDDEDAFRSAVDWHHSRLRAAAGGDVELRGVEGGXXXXXXXXXEVGAGAGARGTFAVFTSHRRGGRAREVLSAALSPSSVMTVAHNREQGGCFFVHASAADVDALLLLPEADXXXXXXXXXXXXXXXXXXXXRLFDGFVAVPPSLKLAPSLLDLDDDGISPGK-------------ALRRNSGVEGLVVVLLSPPL-ASSADDEDGLALVERWRRDWSSASLDLHSLLFWSAEDGHPRHCSGEREGEEDXXXXXXXXXXXXXAAAEVLAREWGGAARTVHALAERLGGVSPAEACGWNDVRVEAEGTGVGTRRRSSSSGWVTLRNIGHLLPGESPDKSQPTTRPGSSXXXXXXXXXXXXXXXXDSEREKKACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRDA--DGSLGT-----------GDFQYDLSQRKVVQYIEGNMGSPD---------------------FIDDDEGGHGTHVAGSVAGSIYSGWTGPNACPDGVAGATADQTAISCVGACLAPSVLAEYVANNIF-----DLGALCPEYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDVGTSDL-LEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLGD-DISDCSISAPGLSKNSLAVGSSMSGGARLSPGE----MDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGGDGS--CELDKNSGTSMSCPIAAGAAALVRQYFVDG-FYSDDLEARGGLCTATSRWACDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDADGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGGPGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTMSGMCSGDEASCSLDNRNNVERIVVPASDVTAGTWTVRVSAGNGLVEGGLQSYALAVSLPISSG 3607
            L+   + L T ++  F     AF +A  W+  RLR +          + G         E G G   R TF+              L   LSP +V  V+H+ + G CF   AS A   A++   E                         + F   P +LKLAP L+D  +     G+                R S VEGL V L    L A SA  E   + +     D  SAS+DLHS  FWS     P    GE                       V AR+W  AA  VH L+E  G  SP + C W++V +   G              + +  + HLL                                 +S     AC MGLVSF A RPEV RVS      + NE A  I+Q ASAT TP+ D G+DG+G+V+QVVD+G+ + SCFF +       DHGY  +    GS G+           GDF Y   +RKV+QYIE  + +PD                     F  DD  GHGTH AGS AG+  +        P      + D+T + CVG C+  S   + + +        DL  LCP Y CD   +  CLSD+  ETL    G+A  AKL+I DV      L + +G  +WE    AG ++HS S G  D  CT  +  + +D++ ++NPE+LLIFAAGN G+ + S C++++P + KN LAVG+S SG  R+   E    +D V+DFSS G T DGRIKP++VAPG  V+SAA+ G  S  C L    GTSMSCPI AGAAA+VRQYFVD  FY+ D+ ARG  C+    + C+ FSPS A VKA+LI+ A+ MG S+ PD  RGFGRIHLE  MP EGE   AL+V DA  T+        +A   V+E  F V  +  A +E         +RATL+W+D  AT++S++QL++DLDL +  P GT + M G       S   DNRN  ER++V A DV  GTWTV V + N LV    QSY+L V+  IS+G
Sbjct:   34 LELVENGLATTAKDDFNGHARAFEAAATWNGERLRRS----------QDGTRAPHLCCAEYGHG---RETFSR-------------LKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFG---------------------LESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAE---SFIIDLLGDLMSASVDLHSTNFWS----DPVMAGGEH---------------LSSHGGAVRARDWSKAASVVHELSEAAG-TSPGDICSWDEVALIQAG-----------DDSLLVTGLDHLL---------------------FSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEE-VDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIE--LVNPDSDYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLND-------PAETLTCSGDET-LGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGS-TDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLG-GDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVISTEGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARG-FCS--DGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTS--------IAEDTVQEYLFDVDAD--AGLE---------LRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWG-------SDVTDNRNVNERVIVDADDVETGTWTVLVYS-NSLVTDS-QSYSLVVNGAISAG 942          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 442 bits (1137), Expect = 8.020e-128
Identity = 380/1088 (34.93%), Postives = 510/1088 (46.88%), Query Frame = 2

Query:  629 LQRFHDVLETNSRRYFGDDEDAFRSAVDWHHSRLRAAAGGDVELRGVEGGXXXXXXXXXEVGAGAGARGTFAVFTSHRRGGRAREVLSAALSPSSVMTVAHNREQGGCFFVHASAADVDALLLLPEADXXXXXXXXXXXXXXXXXXXXRLFDGFVAVPPSLKLAPSLLDLDD------------DGISPGKALR-RNSGVEGLVVVLLSPPLASSADDEDGLALVERWRRDWSSASLDLHSLLFWSAEDGHPRHCSGEREGEEDXXXXXXXXXXXXXAAAEVLAREWGGAARTVHALAERLGGVSPAEACGWNDVRVE-AEGTGVGTRRRSSSSGWVTLRNIGHLLPGESPDKSQPTTRPGSSXXXXXXXXXXXXXXXXDSEREKKACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYL-------------------RDADGSLGT---------------------GDFQYDLSQRKVVQYI--------EGNMG---------------SPDFIDDDEGGHGTHVAGSVAGSIYSGWTGPNACPDGVAGATADQTAISCVGACL---APSVLAEYVANN--IFDLGALCP---EYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDVGTSDL-LEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLGDDI-SDCSISAPGLSKNSLAVGSSMSGGARLS--PG-EMDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGGD---GSCELDKNSGTSMSCPIAAGAAALVRQYFV-DGFYSDDLEARGGLCTATSRWA-CDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDADGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGGPGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTMSGMCSGDEASCSLDNRNNVERIVVPASDVTAGTWTVRVSAGNGLVEGGLQSYALAVSLPISSG 3607
            L+   + L T ++  F     AF +A  W+  RLR       E R              E G G   R TF+              L   LSP +V  V+H+ + G CFF  AS A   A++   E                         + F   P +LKLAP L+D               D +S     R R S VEGL + L    LAS + + +  A +     D   AS+DLHS  FWS      ++  G  +                     V AR+W  AA  VH L+E  G  SP + C WN V V  AEG              + +  + HLL                                 +S     AC MGLVSFLAS+PEV RV+      L N  A   +Q A+ T TP+ D G+DG+GQVVQV+DSGV + SCFF +  +     HG+                     D D SLG                      GDF     +RK++QYI        EG+ G               +  F  D E GHGTHVAGSVAG+  +      +C D           +SCVGAC+     + + +    N  I DL  LCP   +Y+CD   +  CLSDD +ETL    G+A  AKLAI D    +  L   VG  +WE    A  ++HS S+G  D  C++    V +D W   NPE+LLIFAAGN G+   S C++S+P ++KN LAVG+S SG  RLS  PG  +D V+ FSS GPT D RIKP+++APG  ++SAA+ G     SC L   +GTSMS PI AGA+A++RQYF+ + F++ D+ ARG  C     WA C+ FSPS A +KA++I+ A+ MGGS++PDG RGFGRIHLE+ MP  GE   AL+V D++ T+      +        E +F V+    AT   +       +R TL+W+DP AT  S+VQL+HDLDL +  P+GT +TM        AS   D+ N  ER++V A DV  GTW+V + A N   +  +QSY+L V+  IS G
Sbjct:   24 LELVENALSTTAKDDFNGHARAFEAAATWNGERLRHR---QDETRAPH-------LCCAEYGHG---RETFSR-------------LKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFG---------------------LESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAE--AFISDTLGDLMLASVDLHSTNFWS-----DQNMGGGEQ--------------LSFPGGAVRARDWSKAASVVHELSEAAG-TSPGDICSWNRVSVHHAEGD------------VLLVSGLDHLL--------------------HSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKD--------TETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYG-GDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARG-FC---DEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQ--------EYNFDVN----ATARLD-------LRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMW-------ASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTD--VQSYSLVVNGAISPG 969          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 415 bits (1066), Expect = 6.590e-118
Identity = 331/931 (35.55%), Postives = 443/931 (47.58%), Query Frame = 2

Query: 1181 SGVEGLVVVLLSPPLASSADDEDGLA----------LVERWRRDWSSASLDLHSLLFWSAEDGHPRHCSGEREGEEDXXXXXXXXXXXXXAAAEVLAREWGGAARTVHALAERLGGVSPAEACGWNDVRVEAEGTGVGTRRRSSSSGWVTLRNIGHLLPGESPDKSQPTTRPGSSXXXXXXXXXXXXXXXXDSEREKKACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRDADG----SLGT-----------------GDFQYDLSQRK--VVQYIEGNMGSP-----------------------------------------------DFIDDDEGGHGTHVAGSVAGSIYSGWTGPNACPDGVAGATADQ-TAISCVGACL-APSVLAE------------YVANNIFDLGALCPEYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDVGTSDLLEQAV---GGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLGDDISDCSISAPGLSKNSLAVGSSMSGGARLS--------------PGEMDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGGDGS--CELDKNSGTSMSCPIAAGAAALVRQYFV-DGFYSDDLEARGGLCTATSRWACDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDADGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGGPGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTMSGMCSGDEASCSLDNRNNVERIVVPASDV---------------TAGTWTVRVSAGNGLVEGGLQSYALAV 3586
            SGV GL V L    LA  A +  G +          L + WR +  S SLD+H   FWS         SG      D                EVLAREW  AA  +  L    GG +  + C W+ + V             +    +T++ + HLLP                                +++  K AC M L+SFLASRPEV RVS     +L N  A   VQ AS T +P+ D G+DG+G+V+Q+VD+G+   SCFF +     G  HGY  +  G    S G+                 GDF +DLS+RK  VV     +  SP                                               +F +DD GGHGTH AGS AG++ S    P+    G      ++   +SCVG C+       E            +      DL  LCP  +CD      CL DD +ETL+   GVA  A+LAI D+  +D     +   G  MWE +  AG++L S SWG     C    L V FD++ ++N + LL+FAAGN GDD S C++  P L+KN LAVG+S SG  RLS                ++D ++ FSS GPT D RIKP++VAPG  ++SAA  GDG+  C L + +GTSMS PI AGAAALVRQYF  + FY+ D+E RG  C +   + C+PF+PS A VKAMLI+ A+ MGGS +PDG RGFGR+HLE+ MP  GE   AL+V D+  T+T        A   + E SF V  + AA ++         +RATL+W+DP AT  SSVQL+HDLDL +  P G  ++M           ++D+ NN ER++VPAS++               + GTWTVRV +   L     QSY+L V
Sbjct:  222 SGVTGLTVELAPGVLA--ATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWS---------SGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVV-----------HTDDDLLTMKGMDHLLP---------------------EGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSS---PSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDI-INDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYA-YCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERG-FCDS---FGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTST--------AELTLTEYSFAV--DAAAGID---------LRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMW-------RDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKR-LAGNETQSYSLVV 1073          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 398 bits (1023), Expect = 1.850e-116
Identity = 281/678 (41.45%), Postives = 380/678 (56.05%), Query Frame = 2

Query: 1745 ACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRDADGSLGT----------------GDFQYDLSQRKVVQYIEGNMGSPD----------------FIDDDEGGHGTHVAGSVAGSIYS-GWTGPNACPDGVAGATADQTA----------ISCVGACL-----APSVLAEYVANNIFDLGALCPEYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDVGTSDL-LEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLG-DDISDCSISAPGLSKNSLAVGSSMSGGARL----SPGEMDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGGDG--SCELDKNSGTSMSCPIAAGAAALVRQYFVDG-FYSDDLEARGGLCTATSRWACDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDADGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGGPGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTMSGMCSGDEASCSLDNRNNVERIVVPASDVTAGTWTVRVSAGNGLVEGGLQSYALAVSLPISSG 3607
            AC MGLVS LA RPEV RVS  P   + N  A  IVQ ASAT TP+ D G+DG+G+++QV+DSG+ + SC+F + +     DHGY  D                       GDF Y   +RK++QYI+  + +PD                F+  D+ G       ++     S    G +A     AGAT +  A          +SCVGAC+        +++        DL  LCP Y CD    + CLSDD  ETL    G+A  AKLAI DV   D     ++G  +WE    AG +LHS S G  D  C++    +  D++ ++N EHLLIFAAGN G ++ S C+I+ PG+ KNSLA+G+S SG  R+        ++ V++FSS G T D RIKP++VAPG  V+SAA+ G    SC L    GTSMSCPI AGAAA++RQYF+D  FY  D+ ARG  C+    + C+ FSPS A VKA+LI+ A+ MGGS++PDG RGFGR+HLE+ MP EGE    L+V DA  T+ + G         ++E  F V          +A+ G   +RATL+W+DP +TA+S+VQL+H+LDL +  PDGTT+ M G       S + DNRN  ER++V A+DV  GTWTV V A + + +   QSY+L V+  IS G
Sbjct:   30 ACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEE-VDHGYYFDELAQAAVYSSLYSSDETATIFNGGDFSYYPERRKIIQYID--LVNPDSPYSSFYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSA-----AGATLNNPAETITCNVTDTLSCVGACIDLLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLG-ADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARG-FCSDV--FICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGT--------LDEYLFDV----------DADAGLD-LRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWG-------SETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDS--QSYSLVVNGAISPG 667          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 405 bits (1041), Expect = 6.780e-116
Identity = 334/994 (33.60%), Postives = 448/994 (45.07%), Query Frame = 2

Query:  857 HRRGGRAREVLSAALSPSSVMTVAHNREQGGCFFVHASAADVDALLLLPEADXXXXXXXXXXXXXXXXXXXXRLFDGFVAVPPSLKLAPSLLDLDDDGISPGKALRRNSGV-----------------------EGLVVVLLSPPLAS-SADDEDGLALVERWRRDWSSASLDLHSLLFWSAEDGHPRHCSGEREGEEDXXXXXXXXXXXXXAAAEVLAREWGGAARTVHALAERLGGVSPAEACGWNDVRVEAEGTGVGTRRRSSSSGWVTLRNIGHLLPGESPDKSQPTTRPGSSXXXXXXXXXXXXXXXXDSEREKKACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRDADGSLGT------------------GDFQYDLSQRKVVQYIE---------GNMGSP-----DFIDDDEGGHGTHVAGSVAGSIYSGWTGPNACPDGVAGATADQTAISCVGACLAPSVLAEYVANNIFDLGALCPEYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDVGTSDL-LEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLG--DDISDCSISAPGLSKNSLAVGSSMSGGARLSPGEMDD--------------VSDFSSRGPTFDGRIKPDIVAPGHFVFSAA-NGGDGSCELDKNSGTSMSCPIAAGAAALVRQYFVDG-FYSDDLEARGGLCTATSRWACDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDADGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGGPGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTMSGMCSGDEASCSLDNRNNVERIVVPASDVT----AGTWTVRVSAGNGLVEGGLQSYALAVSLPIS 3601
            H  G  A   L   L   +V  V+     G CF V AS A+   L    +                        F  F A P +LK+AP LLD    G   G A  R++                          GL+V L    L S S  D      ++   +  SS SLDLH++  WS                               AA  +  REW  AA  V+  +E     +P++ CGW  V V      V           + +  + HLL                                 ++   + AC MGLVSFLASR EV +V+   R    N  A   +Q AS  STP+ D G+DGSG+V+Q++D+GV + +CF  +   T   +HGY  D   S                     GDF YD  +RKV+QYI+          + G P      F+ DD+ GHGTH A +VAGS  S      +C DG          I C G C+  S      A+   D+  LCP + C       CL DD ++TL    G+A  AK+A +DV   D       G  +WET   AG ++HS S+G  D  C +  L V +DE+ ++N EHLL+F+AGN G   D   C+I +PG++KN L VGS+ SG  RLS  ++D               VS+FS  G T DGRIKP++VAPG  ++SA+ NGGD SCEL   +GTS S  +A+GAAA+VRQYF D  FYS D+E+RG  C     + CD FSP    VKAMLI+ A+ MGG  +PD +RGFGRIHLE+ MP  G    AL+V D+               +  E+  F  + +  A +E         +RATL+W DPAA++ S  QL++DLDL +  PDGT +     C  D+      N N  ER++VPA  V        W VRV  G     G   S++L V+  IS
Sbjct:   72 HGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWED------------------------FTTFGAFPAALKIAPGLLD---HGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWS-------------------DLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQ-ETPSQVCGWEQVVVHHAANDV-----------LLVAGLDHLL---------------------YDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTE-VEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADG-------SEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCS---GDQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGV-DLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDSSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRG-FCD--QGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEV----------YSDEKLKFYFNVDPDAGME---------LRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRY-----CMWDDNCWDTSNVN--ERVIVPAEVVAQFSATDEWEVRVR-GRDFTVGDTVSFSLVVTGAIS 944          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 396 bits (1018), Expect = 9.210e-113
Identity = 358/1153 (31.05%), Postives = 497/1153 (43.10%), Query Frame = 2

Query:  602 LSASSS---GMVLQRFHDVLETNSRRYFGDDEDAFRSAVDWHHSRLRAAAGGDVELRGVEGGXXXXXXXXXEVGAGAGARGTFAVFTSHRRGGRAREVLSAALSPSSVMTVAHNREQGGCFFVHASAADVDALLLLPEADXXXXXXXXXXXXXXXXXXXXRLFDGFVAVP--PSLKLAPSLLD-----------LDDDGISPGKALRRNSGVEGLVV-------VLLSPPLASSADDED-GLALVERWRRDWSSASLDLHSLLFWSAEDGHPRHCSGEREGEEDXXXXXXXXXXXXXAAAEVLAREWGGAARTVHALAERLGGVSPAEACGWNDVRVEAEGTGVGTRRRSSSSGWVTLRNIGHLLPGESPDKSQPTTRPGSSXXXXXXXXXXXXXXXXDSEREKKACLMGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRDA-----DGS----LGTGDFQYDLSQRKVVQYIE---------------------GNMGSPDFIDDDE------------------------------------------------------------GGHGTHVAGSVAGSIYS-GWTGPNAC-PDGVAGATADQTAISCVGACLAPSVLAEYVANNIFDLGALCPEYECDTYW---SSVCLSDDRSETLEGAKGVAPNAKLAILDVGT--SDLLEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLG--DDISD---CSISAPGLSKNSLAVGSSMSG----------------------------------------GARLSPGEMDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGG-DG-SCELDKNSGTSMSCPIAAGAAALVRQYFVD-GFYSDDLEARGGLCTATSRWACDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDA---DGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGGPGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTM--SGMCSGDEASCSLDNRNNVERIVVPASDVTAGTWTVRVSA 3538
            L ASSS   G+ L +   +  +++   F   + A+ +A  W++ RL                            A  G+   +     +++G  A   L  A + S+V  V++    G CF V A  +   A++  PE                        FD   A P  PSLKLA SLLD            DD  +S   A  R++  EG+ +       V LSP + ++   E    A +  W  D +S S+++    FWS  D         R                         REW  AA  V  LA +    +  E CG+  +++           R  +   V +  + HLLPG    K  P+                           + AC MGL+S LA++PEV RVS L  A L N VA  +VQ A+ T TP+ D G+DG+G+V+QVVDSG+ + SCFF +       +HG+L D      DG+    LG   F +D+S+RK+VQYI+                       +G P   DD E                                                            GGHGT  AGS AG+I +       AC  D + G         C G C++ S +   + ++ FDL   CP Y CD      SS CLSDD  E L    GVAP A++++ DV    +++     G  +W ++   GA++H+ SWG     C + +    +D + ++NPEHLLIFAAGN G  +DI +   C++ +P L KNSLAVG++ SG                                         A   P  +D V+ FSS+GPT DGRIKPD+VAPG  V SA++ G DG SC+L  + GTS SCP+ AGAAALVRQYF D  F++ D+ +RGG    +S   C  F+PS A VKAM+I+ A  MGGS++P+G RGFGR+HLE+ MP +G     L V +A   DG  T      G A  E                          +RATLAW+DP  TA S+ QL HDLDL +  P G  +TM  SG+          D  N VER++VP   + +G W+V VSA
Sbjct:    2 LVASSSPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYNDRL-------------------------SYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPED-----------------------FDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTR------------------------VREWSRAAAVVDGLASKSAR-TVGEICGFGKLKL-----------RHVADDLVLVEGMNHLLPG----KRFPS-------------------------ETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADD-DGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPG---------CAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGAT-LCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIADGEITELVTVDGDAGVE--------------------------LRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVD---------DTVNVVERVIVPVESMESGVWSVVVSA 995          

BLAST of mRNA_P-littoralis_Contig83.71.4 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 386 bits (992), Expect = 5.100e-112
Identity = 284/702 (40.46%), Postives = 380/702 (54.13%), Query Frame = 2

Query: 1754 MGLVSFLASRPEVARVSALPRAELSNEVAARIVQGASATSTPIWDRGVDGSGQVVQVVDSGVAQGSCFFRNSATTFGPDHGYLRD-------------------------------ADGSLGT----------GDFQYDLSQRKVVQYI--------EGNMG--------------SPDFID-DDEGGHGTHVAGSVAGSIYSGWTGPNACPDGVAGATADQTAISCVGACL-------APSVLAEYVANNIFDLGALCP---EYECDTYWSSVCLSDDRSETLEGAKGVAPNAKLAILDVGTSDL-LEQAVGGTMWETSGGAGARLHSGSWGFPDDPCTMDDLTVSFDEWAFQNPEHLLIFAAGNLGD-DISDCSISAPGLSKNSLAVGSSMSGGARLS--PGE-MDDVSDFSSRGPTFDGRIKPDIVAPGHFVFSAANGGD---GSCELDKNSGTSMSCPIAAGAAALVRQYFV-DGFYSDDLEARGGLCTATSRWA-CDPFSPSGALVKAMLIHGADAMGGSTDPDGDRGFGRIHLESAMPFEGEDLWALYVEDADGTTTSAGQARGVAAFEVEERSFIVSEEDAATVEAEAEGGPGGIRATLAWMDPAATASSSVQLIHDLDLFLDGPDGTTWTMSGMCSGDEASCSLDNRNNVERIVVPASDVTAGTWTVRVSAGNGLVEGGLQSYALAVSLPISSG 3607
            MGLVSFLAS+PEV RV+      L N  A   +Q A+ T TP+ D G+DG+GQVVQV+DSGV + SCFF +  +     HG+  +                               AD   G+          GDF     +RK++QYI        EG+ G              S D  + DDE GHGTHVAGSVAG+  +      +C D           +SCVGAC+          +   YV   I DL  LCP   +Y+CD   +  CLSDD +ETL    G+A  AKLAI D       L   VG  +WE    A  ++HS S+G  D  C++    V +DEW   NPE+LLIFAAGN G+   S C+I +P ++KN LAVG+S SG  RLS  PG  +D V+ FSS GPT D RIKP+++APG  ++SAA+ G     SC L    GTSMS PI AGA+A++RQYF+ + F++ D+ ARG  C     WA C+ FSPS A +KA++I+ A+ MG S++P+G RGFGRIHLE+ MP  GE   AL+V D++ T+        +     +E +F V+    AT   +       +R TL+W+DP AT  S+VQL+HDLDL +  P+GT +TM        AS   D+ N  ER++V A DV  GTW+V + A N   +  +QSY+L V+  IS G
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCND--------TETMSCVGACVDDDEANDVDDLATPYV--EIADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYG-GDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARG-FC---DEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTS--------IPELTRQEYNFDVN----ATAGLD-------LRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMW-------ASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTD--VQSYSLVVNGAISPG 659