mRNA_P-littoralis_Contig65.71.1 (mRNA) Pylaiella littoralis U1_48

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1108 bits (2866), Expect = 0.000e+0
Identity = 599/941 (63.66%), Postives = 711/941 (75.56%), Query Frame = 1

Query:   10 AASGWLCPSLRVVERALSTTAKDDFNGDAHMFEAAVKWDGEKLFASSSSSSSSNQDGVIRAPHLACAAYGRGSEILSDLKDLLSPEAVRLVSHSSEDGVCFIATASPAQAAAIAAEPDQFGLESFAPFPSAMKLAPGLLEHQQSSHL--------GRLSARHGTSMRLSNVEGLTVELSPGTLPAHSSEADPLDPLIGDLLRDLMS*SLDLHRMNFWSDPAMFEVVHVGATPGGAARGRDWSMAATAVHELSKAGRTSPGDICSWNSVAVNQVATDVLLVSGLDHLLYSGKEAGGN-EKAATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFIDGDGQEATHGYYYDAM--GSPASSSFSSYSTYGVFQGGDFSFYPERRKIIQYIDMIKPDS------TTSIPGYYLYPADGFGDDEKAGHGTHTAGTAAGATLNTPAETVTCGTGETLSCVGDCIDATGSDDDLVSYYEQYA--DIDRLCPMLECDDDSELCLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDSAGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGENDFNAECTIGSPAIAKNVLAVGATSTGETR-LNMDGIHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGTDAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCNDGFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWSSGEKDTANVNERVVVDAADVESGTWTVSVSSNSLTTPLQSFSLVVNGAVSPGTGEGAT 2772
            AA  W  PSL +VE  L+TTAKDDFNG A  FEAA  W+GE+L  S        QDG  RAPHL CA YG G E  S LK  LSP+AVR+VSHS + G CF+ATAS AQA+AI  + +QFGLESF+PFPSA+KLAPGL++H +S  +         RL ARHG +MR+SNVEGL V+L+P TLPA S+ A+     I DLL DLMS S+DLH  NFWSDP M    H+ ++ GGA R RDWS AA+ VHELS+A  TSPGDICSW+ VA+ Q   D LLV+GLDHLL+SG+ AGG+ E+ +TELH ACFM LVSF A R +VLRV P H+KR L+ AAR IIQ+A++TDTPLT AGL+GTGE+IQV+DTGLDETSCFFID  G+E  HGY+++ +  GS  SS       Y VF+ GDF++  ERRK+IQYI+++ PDS      TT    Y   P D FG D+ AGHGTHTAG+AAGATLN PAET+TC   ETL CVG CID + + DDLVSY  Q A  D+DRLCPM  CD  ++ CL D+V  TL  NGG+AQGAKL+IFD+F      ++  GNG+WEPCLEAGCK+HS S GGD LC    ++ILYD+FMY+N ENLL+FAAGNDGE +  + CT+ SPAI KNVLAVGA+++G+TR ++ +G   ID VA FSS+GFTTDGRIKPEVVAPGDM+YSAA DGTD+HSCRLWAY GTSMS P+ AGA+AMVRQYFVD +FY ADVTAR FC+DGF+CEGFSPS+ATVKA+LINSANLMG  S PD YRGFGRIHLE GMPL GEG+L LFVADA +TSI+E + QEYLFDVDADAGL  RATLSWID PAT+ SA QLV+DLDL V+SPSGT + MW S   D  NVNERV+VDA DVE+GTWTV V SNSL T  QS+SLVVNGA+S GTGEGA+
Sbjct:   25 AADTWSPPSLELVENGLATTAKDDFNGHARAFEAAATWNGERLRRS--------QDGT-RAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESF---IIDLLGDLMSASVDLHSTNFWSDPVMAGGEHL-SSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQL---IYYPVFEAGDFTYTEERRKVIQYIELVNPDSDYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE-RSVCTMASPAIGKNVLAVGASTSGDTRVISTEGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAISAGTGEGAS 948          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 1048 bits (2711), Expect = 0.000e+0
Identity = 571/931 (61.33%), Postives = 673/931 (72.29%), Query Frame = 1

Query:   31 PSLRVVERALSTTAKDDFNGDAHMFEAAVKWDGEKLFASSSSSSSSNQDGVIRAPHLACAAYGRGSEILSDLKDLLSPEAVRLVSHSSEDGVCFIATASPAQAAAIAAEPDQFGLESFAPFPSAMKLAPGLLEHQQS----------SHLGRLSARHGTSMRLSNVEGLTVELSPGTLPAHSSEADPLDPLIGDLLRDLMS*SLDLHRMNFWSDPAMFEVVHVGATPGGAARGRDWSMAATAVHELSKAGRTSPGDICSWNSVAVNQVATDVLLVSGLDHLLYSGKEAGGN-EKAATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFIDGDGQEATHGYYYDAMGSPA--SSSFSSYSTYGVFQGGDFSFYPERRKIIQYIDMIKPDSTTSIPGYYLYPADGFGDDEKAGHGTHTAGTAAGATLNTPAETVTCGTGETLSCVGDCIDATGSDDDLVSYYEQY--ADIDRLCPMLECDDDSEL-CLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDSAGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGENDFNAECTIGSPAIAKNVLAVGATSTGETR-LNMDGIHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGTDAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCNDGFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWSSGEKDTANVNERVVVDAADVESGTWTVSVSSNSLTTPLQSFSLVVNGAVSPGTGEGAT 2772
            P+L +VE AL TTAKDDF G    FEAA  W+GE+L  S   +         RAPHLACA YG G E  S LK  LSPEAVR+VSHS + G CF A+AS AQAAAI  + +QFGLE+F+PFPS +KLAPGL++H +S          S L RLSARHG  MR  NVEGL+VEL+PGTL A SSEA      I  +L DLMS S+DLH  NFWSDP M       ++P GA R RDW  AA+ VHELS+A  T PGDICSWN + V+    D LLVSGLDHLL+SG+ AGG+ E+ +TELH ACFM LVSFLA R +VLRV P  +KR L+AAAR +IQ+A++TDTPLT AGL+GTGEIIQV+D+GLDETSC+FID  G+E  HGYY+D +G  A  SSS+SS  T  +F GGDFS+YPERRK                                AGHGTHTAG+AAGATLN PAET+TC   +TLSCVG CID   + DDLVS   Q    D+DRLCPM  CD+ ++  CL DDV  TL+ NGG+AQGAKLAIFD+F        S GN LWE C EAGCK+HSNS G D  C +   DIL D+FMY+N ENLL+FAAGN+G  +  + CTI +P I KN LA+GA+++G+TR ++  G   ID VA FSSWGFTTD RIKPEVVAPGD +YSAA DGTD HSCRLWAY GTSMS P+ AGA+AM+RQYFVD +FY ADVTAR FC+DGF+CEGFSPS+ATVKA+LINSANLMGG S+PDG+RGFGR+HLE GMPL GEG+L LFVADA +TSI+  + +EYLFDVDADAGL  RATLSWIDPPAT  SA QLVH+LDL V+SP GTT+ MW S   D  NVNERV+VDA DV +GTWTV V +N+L T  QS+SLVVNGA+SPGTGEGA+
Sbjct:   27 PALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEET---------RAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSF---INHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSSYSSDETATIFNGGDFSYYPERRK--------------------------------AGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE-RSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAISPGTGEGAS 912          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 1017 bits (2629), Expect = 0.000e+0
Identity = 571/968 (58.99%), Postives = 695/968 (71.80%), Query Frame = 1

Query:   31 PSLRVVERALSTTAKDDFNGDAHMFEAAVKWDGEKLFASSSSSSSSNQDGVIRAPHLACAAYGRGSEILSDLKDLLSPEAVRLVSHSSEDGVCFIATASPAQAAAIAAEPDQFGLESFAPFPSAMKLAPGLLEHQQ--------SSHLGRLSARHGTSMRLSNVEGLTVELSPGTLPAHSSEADPLDPLIGDLLRDLMS*SLDLHRMNFWSDPAMFEVVHVGATPGGAARGRDWSMAATAVHELSKAGRTSPGDICSWNSVAVNQVATDVLLVSGLDHLLYSGKEAGGNEKA--ATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFI-DGDGQEATHGYYYDAMG-SP--ASSSFSS-----------------------YSTYGVFQGGDFSFYPERRKIIQYIDMIKPDSTTSIPG----------YYLYPADGFGDDEKAGHGTHTAGTAAGATLNTPAETVTCGTGETLSCVGDCIDATGSDD--DLVSYYEQYADIDRLCPML---ECDD-DSELCLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDS-AGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGENDFNAECTIGSPAIAKNVLAVGATSTGETRLNMDGIHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGT-DAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCNDGFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWSSGEKDTANVNERVVVDAADVESGTWTVSVSSNSLTTPLQSFSLVVNGAVSPGTGEGA 2769
            PSL +VE ALSTTAKDDFNG A  FEAA  W+GE+L          ++    RAPHL CA YG G E  S LK  LSPEAVR+VSHS + G CF A+AS AQA+AI  + +QFGLESF+PFPSA+KLAPGL++H Q        ++ L RLSARHG  MR+SNVEGL++EL+PGTL + S EA+     I D L DLM  S+DLH  NFWSD  M     + + PGGA R RDWS AA+ VHELS+A  TSPGDICSWN V+V+    DVLLVSGLDHLL+SG+ AGG      + ELH ACFM LVSFLAS+ +VLRV P  S+  L+A+ARA IQ+AT TDTPLT AGL+GTG+++QV+D+G+DE+SCFF  D  G    HG+Y++  G +P   SSSFSS                       Y     F GGDF+ +P+RRKIIQYI++IK D T    G          Y   PAD F  D +AGHGTH AG+ AGATLNTPAE ++C   ET+SCVG C+D   ++D  DL +   +  D+DRLCP+    +CD  ++E CL DDV  TL  NGG+AQGAKLAIFD F  +N GL    GNGLWEPC EA CKIHSNS+GGD  C + PND+LYD +M  N ENLL+FAAGNDGE    + CT+ SPAIAKNVLAVGA+++G+TRL+      ID VA FSS+G T+D RIKPEV+APGD IYSAA DG+ D+HSCRLWAYAGTSMSTP+ AGASAM+RQYF++ +F+A D+TAR FC++   CE FSPSSAT+KA++INSANLMGG S+PDG RGFGRIHLE GMPL GEG+L LFV+D+  TSI E ++QEY FDV+A A L  R TLSW+DPPATT SA QLVHDLDL VISP+GT +TMW+SG  D+ANVNERV+VDA DVE+GTW+V + +++LTT +QS+SLVVNGA+SPGTG GA
Sbjct:   22 PSLELVENALSTTAKDDFNGHARAFEAAATWNGERL---------RHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAF---ISDTLGDLMLASVDLHSTNFWSDQNMGGGEQL-SFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAF-YDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERR-RSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGVGA 974          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 910 bits (2353), Expect = 0.000e+0
Identity = 513/909 (56.44%), Postives = 617/909 (67.88%), Query Frame = 1

Query:   31 PSLRVVERALSTTAKDDFNGDAHMFEAAVKWDGEKLFASSSSSSSSNQDGVIRAPHLACAAYGRGSEILSDLKDLLSPEAVRLVSHSSEDGVCFIATASPAQAAAIAAEPDQFGLESFAPFPSAMKLAPGLLEH-----QQSSHLGRLSARHGTSMRLSNVEGLTVELSPGTLPAHSSEADPLDPLIGDLLRDLMS*SLDLHRMNFWSDPAMFEVVHVGATPGGAARGRDWSMAATAVHELSKAGRTSPGDICSWNSVAVNQVATDVLLVSGLDHLLYSGKEAGGNEKAATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFIDGDGQEATHGYYYDAMGSPASSSFSSYSTYGVFQGGDFSFYPERRKIIQYIDMIKPDSTTSIPGYYLYPADGFGDDEKAGHGTHTAGTAAGATLNTPAET-VTCGTGETLSCVGDCIDA--TGSDDDLVSYYEQYA----DIDRLCPMLECDDDSE-LCLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDSAGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGEN-DFNAECTIGSPAIAKNVLAVGATSTGETRLNM-----------DGIHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGTDAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCND-GFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWS-SGEKDTANVNERVVVDAADVESGTWT 2676
            PSL VVE +L TTA+D F+G A  FE A +W GE L         S ++   RAPHL C  Y RG + L  L++LLSP AVR V+HSS  G CF  TAS A+A A++  P  +GL S +PFPSA+K+APG+LEH     Q S   GRL+  HG  MR  NVEGL +ELSPGTLPAHS EA      I +LL DLMS S+DLH  N WSDP      H+  TP GA R R+WSMAAT VH+L+ AGRTSPGDICSW+S++++    D+LLVSGLDHLL+    A G+++ ATEL  ACFM LVSFL++R +VLRV P H+ R L+A AR IIQ+AT T+ PLT AGL+GTG++IQV+DTGLDETSCFFID DG+E  HG+YY+A+G                                                 + P+     D+  GHGTH AG+A GAT+ TPAET  TC   E   CVG CIDA  T   DDL +   +Y+    DIDRLCPM++CDD ++  CL DDVG TLA +GG+AQGAKLA FD+F+  ++G    GNGLWEPCLEAGCKIHS S G D  C V   D+LYD+FMY N ENL++ AAGN G+  D    CT+ SPAI KN LAVGATS+GETR+             +G  D+D VA FSS+G T DGRIKPEVVAPGD IYSA  DG+D HSCRL A +GTSM+ P+ AGASAMVRQYF+D SFY AD  AR + +D G  CE FSPSSATVKA+LINSANLMGG S+PDGYRGFGRIHLE GMPL GEG+L LFVAD+  TS+ E   Q+++FDVDA+A L FRATLSWIDPPA++ S  QLVHDLDL V+SPSG TH MW+ SGE D+ NVNERV++ A DVESGT+T
Sbjct:   32 PSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEIL--------RSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSP-YYGLTSISPFPSALKVAPGVLEHGVSLDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMF---IAELLDDLMSDSIDLHGGNVWSDPVAANGEHL-TTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALGE------------------------------------------------IIPSSSSARDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 877 bits (2265), Expect = 9.940e-306
Identity = 516/950 (54.32%), Postives = 628/950 (66.11%), Query Frame = 1

Query:    4 GSAASGWLCPSLRVVERALSTTAKDDFNGDAHMFEAAVKWDGEKLFASSSSSSSSNQDGVIRAPHLACAAYGRGSEILSDLKDLLSPEAVRLVSHSSEDGVCFIATASPAQAAAIAAEPDQFGLESFAPFPSAMKLAPGLLEH-----QQSSHLGRLSARHGTSMRLSNVEGLTVELSPGTLPAHSSEADPLDPLIGDLLRDLMS*SLDLHRMNFWSDPAMFEVVHVGATPGGAARGRDWSMAATAVHELSKAGRTSPGDICSWNSVAVNQVATDVLLVSGLDHLLYSGKEAGGNEKAATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFIDGDGQEATHGYYYDAMGSPASSSFSSYSTYGVFQGGDFSFYPERRKIIQYIDMIKPDS------TTSIPG---YYLYPADGFGDDEKAGHGTHTAGTAAGATLNTPAET-VTCGTGETLSCVGDCIDA--TGSDDDLVSYYEQ----YADIDRLCPMLECDDDSE-LCLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDSAGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGE-NDFNAECTIGSPAIAKNVLAVGATSTGETRLNM-----------DGIHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGTDAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCND-GFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWS-SGEKDTANVNERVVVDAADVESGTWTVSVSSNSLTTPLQSFSLVVNGAV 2745
            G +A+    PSL VVE +L TTA + F+G A  FE A +W G+ +         S ++   RAPHL C  Y RG E LS L++LLSPEAVR V+HSS  G CF  TAS A+A A++  P  +GL S +PFPSA+K+APG+LEH     Q S   GRL+  HG  MR  NVEGL +ELSPGTLPAHS +A      I +LL DLMS S+DLH  N WSDP      H+  TP GA R R+WSMAAT VH+L+ AGRTSPGDICSW+S++++    D+LLVSGLDHLL+    A G+++ ATEL  ACFM LVSFL+SR +V RV P H+ R L+A AR IIQ+AT T  PLTAAGL+GTG++IQV                                                            IQYI++IK DS      +T+  G   +++ P DGF  D+   HGTHTAG+A GAT+ TPAET  TC   E   CVG CIDA  T   DDL++   +     ADIDR+CPML CD+++E  CL D V  TLA +GG+AQGAKL+ FDI+          GNGLWEPCLEAGCKIHS S+G D  C V   D+LYD+FMY N ENL++ AAGN GE  D    CT+ SPAI KN LAVGATS+GETR+ +           +G  D+D VA FSS+G T DGRIKPEVVAPGD +YSA  DG+D HSCRL A +GTSM+ P+ AGASAMVRQYF+D SFY AD  AR +C+D G  CE FSPSSATVKA+LINSANLMGG S+PDG+RGFGRIHLE GMPL GEG+L LFVAD+  TS+ E S Q+++FDVDA+A L FRATLSWIDPPA++ S  QLVHDLDL V+SPSG TH MW+ SGE D+ NVNERV++ A +VESGT+TV VS+N L T  Q++SLVVNGA+
Sbjct:   23 GGSANSLPYPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDII--------RSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNP-YYGLTSISPFPSALKVAPGVLEHGVSRDQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMF---IAELLDDLMSDSIDLHGGNVWSDPVAANGEHL-TTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQV------------------------------------------------------------IQYINLIKADSVSGPASSTTSGGNRVWWVSP-DGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 784 bits (2025), Expect = 1.310e-272
Identity = 422/671 (62.89%), Postives = 505/671 (75.26%), Query Frame = 1

Query:  826 SGLDHLLYSGKEAGGN-EKAATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFIDGDGQEATHGYYYDAMGSPA--SSSFSSYSTYGVFQGGDFSFYPERRKIIQYIDMIKPDST-----TSIPGY--YLYPADGFGDDEKAGHG--------THTAGTAAGATLNTPAETVTCGTGETLSCVGDCIDATGSDDDLVSYYEQYA--DIDRLCPMLECDDDSEL-CLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDSAGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGENDFNAECTIGSPAIAKNVLAVGATSTGETR-LNMDGIHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGTDAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCNDGFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWSSGEKDTANVNERVVVDAADVESGTWTVSVSSNSLTTPLQSFSLVVNGAVSPGTGEGAT 2772
            +GLDHLL+SG+ AGG+ E+ +TELH ACFM LVS LA R +VLRV P  +KR L+AAAR I+Q+A++TDTPLT AGL+GTGEIIQV+D+GLDETSC+FID  G+E  HGYY+D +   A  SS +SS  T  +F GGDFS+YPERRKIIQYID++ PDS      TS   Y  Y  P D FG+D++A                +AAGATLN PAET+TC   +TLSCVG CID     DDLVS   Q    D+DRLCPM  CD+ ++  CL DDV  TL+ NGG+AQGAKLAIFD+F  +     S GN LWE C EAGCK+HSNS G D  C +   DIL D+FMY+N E+LL+FAAGN+G  +  + CTI +P I KN LA+GA+++G+TR ++  G   I+ VA FSSWGFTTD RIKPEVVAPGD +YSAA DGTD HSCRLWAY GTSMS P+ AGA+AM+RQYF+D +FY ADVTAR FC+D F+CEGFSPS+ATVKA+LINSANLMGG S+PDG+RGFGR+HLE GMPL GEGNL LFVADA +TSI+E +  EYLFDVDADAGL  RATLSWIDPP+T  SA QLVH+LDL V+SP GTT+ MW S   D  NVNERV+VDA DV +GTWTV V +NSL T  QS+SLVVNGA+SPGTGEGA+
Sbjct:    4 TGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVYSSLYSSDETATIFNGGDFSYYPERRKIIQYIDLVNPDSPYSSFYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACIDLLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEE-RSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAISPGTGEGAS 673          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 792 bits (2045), Expect = 1.290e-271
Identity = 467/945 (49.42%), Postives = 599/945 (63.39%), Query Frame = 1

Query:   31 PSLRVVERALSTTAKDDFNGDAHMFEAAVKWDGEKLFASSSSSSSSNQDGVIRAPHLACAAYGRGSEILSDLKDLLSPEAVRLVSHSSEDGVCFIATASPAQAAAIAAEPDQFGLESFAPFPSAMKLAPGLLEH---------------QQSSHLGRLSARHGTSMRLSNVEGLTVELSPGTLPAHSSEADPLDPLIGDLLRDLMS*SLDLHRMNFWSDPAMFEVVHVGATPGGAARGRDWSMAATAVHELSKAGRTSPGDICSWNSVAVNQVATDVLLVSGLDHLLYSGKE-AGGNEKAATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFIDGDGQEATHGYYYDAMGSP----ASSSFSSYSTYGVFQGGDFSFYPERRKIIQYIDMIKPDSTT-SIPGYYLYPADGFGDDEKAGHGTHTAGTAAGATLNTPAETVTCGTG-ETLSCVGDCIDATGSDDDLVSYYEQYADIDRLCPMLECDDDSELCLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDSAGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGENDFNAECTIGSPAIAKNVLAVGATSTGETRLN---MDG--------IHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGTDAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCNDGFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWSSGEKDTANVNERVVVDAADVE----SGTWTVSVSSNSLTT-PLQSFSLVVNGAVSP 2751
            PSL   E+ L TTA  DF+ +A    A+    G++   SS              P+L+C  +G G      L+  L   AV+ VS ++  G CF+ TASPA+AA +++  D     +F  FP+A+K+APGLL+H                     GRLS  HG SM++ +  GL VELSPGTLP+HS         I +LL+ L S SLDLH +  WSD  M    H+ +  G A RGR+WS AAT V+E S++ + +P  +C W  V V+  A DVLLV+GLDHLLY G+E +G +E+ A EL  AC+M LVSFLASRH+VL+V   + K  L+AAARA IQ+A+   TPLT AGL+G+GE+IQ++D G++ET+CF   GDG E  HGYY+D + S      SSS S       F GGDFS+  +RRK+IQYID+I PD+   S  G     AD F  D+  GHGTH A T AG+T+++PAE V+C  G E + C G CI  + +      Y E   DI+RLCP   C  D   CL DD   TL+ +GG+AQGAK+A  D+F  + +  D AGNGLWE C +AGCKIHSNS+G D  C +   D+LYDE+MY+N E+LLLF+AGN G       CTI SP IAKNVL VG+TS+G TRL+   +DG        +  ID V+ FS +GFT DGRIKPEVVAPGDMIYSA+G+G D+ SC L AYAGTS ST VA+GA+AMVRQYF DSSFY+ADV +R FC+ GF C+ FSP + TVKA+LINSAN+MGG ++PD  RGFGRIHLE GMPLGG G++ LFVAD+ +  +    + ++ F+VD DAG+  RATLSW DP A++LS  QLV+DLDL V +P GT + MW     DT+NVNERV+V A  V     +  W V V     T     SFSLVV GA+SP
Sbjct:   25 PSLGFFEQTLLTTAGADFDSEAAFAFASAWQAGDRQGRSS--------------PYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWED---FTTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAG-ALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGE---DINRLCPAFGCSGDQ--CLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 945          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 709 bits (1831), Expect = 6.950e-243
Identity = 395/666 (59.31%), Postives = 486/666 (72.97%), Query Frame = 1

Query:  910 MELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFI-DGDGQEATHGYYYDAMG---SPASSSFSS------------------------YSTYGVFQGGDFSFYPERRKIIQYIDMIKPDSTTSIPG----------YYLYPADGFGDDEKAGHGTHTAGTAAGATLNTPAETVTCGTGETLSCVGDCIDATGSDD--DLVSYYEQYADIDRLCPML---ECDD-DSELCLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDS-AGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGENDFNAECTIGSPAIAKNVLAVGATSTGETRLNMDGIHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGT-DAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCNDGFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWSSGEKDTANVNERVVVDAADVESGTWTVSVSSNSLTTPLQSFSLVVNGAVSPGTGEGA 2769
            M LVSFLAS+ +VLRV P  S+  L+AAARA IQ+AT TDTPLT AGL+GTG+++QV+D+G+DE+SCFF  D  G    HG+Y++  G   S  SSSFSS                        Y     F GGDF+ YP+RRKIIQYI++IK D T    G          Y    AD F  D++AGHGTH AG+ AGATLNTPAE ++C   ET+SCVG C+D   ++D  DL + Y + AD+DRLCP+    +CD  +++ CL DDV  TL  NGG+AQGAKLAIFD F  +  GL    G GLWEPC +A CKIHSNS+GGD  C +  ND+LYDE+M  N ENLL+FAAGNDGE    + CTIGSPAIAKNVLAVGA+++G+TRL+      ID VA FSS+G T+D RIKPEV+APGD IYSAA DG+ D HSCRLWAY GTSMSTP+ AGASAM+RQYF++ +F+A D+TAR FC++   CE FSPSSAT+KA++INSANLMG  S+P+G RGFGRIHLE GMPLGGEG+L LFV+D+  TSI E ++QEY FDV+A AGL  R TLSW+DPPATT SA QLVHDLDL VISP+GT +TMW+SG  D++NVNERV+VDA DVE+GTW+V + +++LTT +QS+SLVVNGA+SPGTG GA
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAF-YDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELR-RSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGVGA 664          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 709 bits (1829), Expect = 2.520e-237
Identity = 472/1072 (44.03%), Postives = 608/1072 (56.72%), Query Frame = 1

Query:   31 PSLRVVERALSTTAKDDFNGDAHMFEAAVKWDGEKLFASSSSSSSSNQDGVIRAPHLACAA--YGRGSEILSDLKDLLSPEAV--RLVSHSSEDGVCFIATASPAQAAAIAAEPDQFGLESFAPFPSAMKLAPGLLEHQQSSHLG-----RLSARHGTSMRL----------------------------------------------SNVEGLTVELSPGTLPAHSSEADP-------LDPLIGDLLRDLMS*SLDLHRMNFWSDPAMFEVVHVGATPG---------GAARGRDWSMAATAVHELSKA-GRTSPGDICSWNSVAVNQVATDVLLVSGLDHLLYSGKEAGGNE-KAATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFIDGDGQEAT-HGYYYDAMGSPASSSFSSYSTYG-------VFQGGDFSFYPERRKIIQYIDMIKPD-------------------------------------STTSIPGYYLYPADGFGDDEKAGHGTHTAGTAAGATLNTPAETVT------CGTGETLSCVGDCIDAT---GSDDDLVSYYEQY-------ADIDRLCPMLECDD-DSELCLDDDVGATLAANGGIAQGAKLAIFDIFS-VENTGLDSAGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDGENDFNAECTIGSPAIAKNVLAVGATSTGETRLNM--------DG---IHDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGTDAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFCNDGFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWSSGEKDTANVNERVVVDAADV---------------ESGTWTVSVSSNSLT-TPLQSFSLVVNGAVSPGT 2757
            P L V+  AL TTA   F      F+AAV+W   +L            D    AP+LACA+    RG    + L  +L   A+  R+V  S+E GVC +ATA+P QA +I      F   S+A FPSA+KLAPGLL+H      G     RL   HG  +R                                               S V GLTVEL+PG L A +               L  +   +LMS SLD+H  +FWS     ++    A  G         G    R+W+ AA  +  L    G  + GD+CSW+ + V     D+L + G+DHLL  G+ AGGNE   A EL  ACFM L+SFLASR +VLRV P  + + L+AAA   +Q+A+ T++P+T AGL+GTGE+IQ++DTGLD  SCFF+D DG E   HGYY++ +G  ASS  S ++++        VF GGDF+F   RRK +  +D   P                                      +T++   Y    A  F +D+  GHGTHTAG+AAGA +++P+ + T      C  G  LSCVG CID     G DD + ++  QY        D+DRLCP L+CDD   E CL DD   TL  +GG+A+GA+LAIFDI +     G+D AGNG+WEP +EAG K+ SNSWG    C     D+L+D+FMY+N + LLLFAAGNDG+ D++  CT+G+PA+AKNVLAVGA+++G  RL+         DG   + DID +A FSS+G T D RIKPEVVAPGDMIYSAAG G   ++CRLW YAGTSMSTP+ AGA+A+VRQYF + +FYA DV  R FC D F CE F+PS+ATVKA+LINSANLMGG ++PDG RGFGR+HLE GMPL GEG+  LFVAD+  TS +E +  EY F VDA AG+  RATLSWIDPPATT S+ QLVHDLDL+V SP+G  ++MW  G  D+ N NERV+V A+++                 GTWTV V S  L     QS+SLVV GA+ P T
Sbjct:   30 PPLSVIRGALLTTAGVTFRHQGE-FKAAVEWHEARL------------DDARPAPYLACASPDSDRGGSGAT-LSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFS--SWAAFPSALKLAPGLLDHPHDDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLV-VDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD-DWSG-CTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFC-DSFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPPT 1081          

BLAST of mRNA_P-littoralis_Contig65.71.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 618 bits (1594), Expect = 9.120e-204
Identity = 418/1038 (40.27%), Postives = 563/1038 (54.24%), Query Frame = 1

Query:   37 LRVVERALSTTAKDDFNGDAHMFEAAVKWDGEKLFASSSSSSSSNQDGVIRAPHLACAAYGRGSEILSDLKDLLSPEAVRLVSHSSEDGVCFIATASPAQAAAIAAEPDQFGLESFAPFPSAMKLAPGLLEH--------------QQSSHLGRLSARHGTSMRLSNVEGLTVELSPGTLPAHSSEADPLDPLIGDLLRDLMS*SLDLHRMNFWSDP----AMFEVVHVGATPGGAARGRDWSMAATAVHELSKAGRTSPGDICSWNSVAVNQVATDVLLVSGLDHLLYSGKEAGGNEKAATELHTACFMELVSFLASRHQVLRVGPTHSKRPLDAAARAIIQTATSTDTPLTAAGLNGTGEIIQVLDTGLDETSCFFIDGDGQEATHGYYYDAMGSPASSSFSSYSTYGVFQGGDFSFYPERRKIIQYIDMIK-PD--STTSIPGYYLYPADGFGD-----------------------------------------------------------------DEKAGHGTHTAGTAAGA-TLNTPAETVTCGTGETLSCVGDCIDATGSDDDLVSYYEQYADIDRLCPMLECDDDSEL----CLDDDVGATLAANGGIAQGAKLAIFDIFSVENTGLDS-AGNGLWEPCLEAGCKIHSNSWGGDGLCLVYPNDILYDEFMYKNTENLLLFAAGNDG--ENDFNAE-CTIGSPAIAKNVLAVGATSTGE---TRLNMDG--IHD--------------------------------IDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAAGDGTDAHSCRLWAYAGTSMSTPVAAGASAMVRQYFVDSSFYAADVTARRFC---NDGFLCEGFSPSSATVKAVLINSANLMGGVSDPDGYRGFGRIHLENGMPLGGEGNLTLFVADAGNTSISEFSQQEYLFDVDADAGLSFRATLSWIDPPATTLSATQLVHDLDLAVISPSGTTHTMWSSGEKDTANVNERVVVDAADVESGTWTVSVSSNSLTTPLQSFSLVVNGAV 2745
            L  ++   +++A DDF      + AA  W  ++L   ++  S+        AP++ACA Y +G   L+ L+   +  AV  VS++  DG CFI TA P+ A A+   P+ F L S AP   ++KLA  LL+H                S H   L + +G  M L NV GL+V LSPG L A   E+   +  + D   DL S S+++ R +FWSD     + ++V  V          R+WS AA  V  L+     + G+IC +  + +  VA D++LV G++HLL  GK      +  +E  TAC+M L+S LA++ +VLRV P  S R  +A A A++Q+AT+T+TPL  AGL+GTGE+IQV+D+GLDETSCFF D DG +  HG+ +D +      + S+     V     F F   RRKI+QYI + K PD  ST+S    + Y +D  GD                                                                 D   GHGT TAG+AAGA +  +  E   C   E   C G CI A+  D       +   D+D  CPM  CD ++++    CL DD    L  +GG+A GA++++FD+  V      S AGN LW   +E G KIH+NSWGG  LC +  ++ LYD FMY+N E+LL+FAAGNDG  E+  N E CT+GSPA+ KN LAVGATS+G    TR   DG  +++                                +D VA FSS G T DGRIKP+VVAPGD + SA+ DGTD HSC+L A  GTS S P+ AGA+A+VRQYF D SF+A DV +R  C   +    C  F+PS+ATVKA++INSA+LMGG S+P+G RGFGR+HLE GMP+ G G   L V +A   +I++    E L  VD DAG+  RATL+W+DPP T LSA QL HDLDL VI+PSG  +TMW SG  DT NV ERV+V    +ESG W+V VS+ +L T  Q +SLVV GA+
Sbjct:   14 LGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGST--------APYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFA-EAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRV----------REWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLL-PGK------RFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVST-----VLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVD---AMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEA---AIADGEITE-LVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1013