mRNA_P-littoralis_Contig59.74.4 (mRNA) Pylaiella littoralis U1_48

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-littoralis_Contig59.74.4
Unique NamemRNA_P-littoralis_Contig59.74.4
TypemRNA
OrganismPylaiella littoralis U1_48 (Pylaiella littoralis U1_48 (Mung))
Homology
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: D7G5K3_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G5K3_ECTSI)

HSP 1 Score: 194 bits (492), Expect = 3.210e-60
Identity = 93/105 (88.57%), Postives = 97/105 (92.38%), Query Frame = 2 
Query:  257 PRLASSAPLQMVESEVKEEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDEVR 571
            P    +  +QMVESEVKEEF+KHMA TLDWGALCKAA+EVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEG LVCPETGRRFPIAEGVPNLMCTEDEVR
Sbjct:   21 PLRIEATDVQMVESEVKEEFVKHMATTLDWGALCKAAAEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGTLVCPETGRRFPIAEGVPNLMCTEDEVR 125
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: A0A836CHT2_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CHT2_9STRA)

HSP 1 Score: 121 bits (304), Expect = 9.880e-32
Identity = 57/103 (55.34%), Postives = 76/103 (73.79%), Query Frame = 2 
Query:  257 PRLASSAPLQMVESEVKEEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDE 565
            P    ++ +QM ES  + +F+KH+A TL+W AL +A  +VG+PE  LP A++  LL D +FL ALHRVLMD+HVV GALVCPE+GRRFPIA G+P++M  E E
Sbjct:   21 PLSIEASEVQMEESPFRPDFVKHIAPTLNWPALRQAVKQVGLPEDMLPEAVSDALLGDEAFLRALHRVLMDVHVVAGALVCPESGRRFPIAHGIPDMMIAEGE 123
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: A0A2P6VL84_9CHLO (Multifunctional methyltransferase subunit TRM112 At1g22270 n=1 Tax=Micractinium conductrix TaxID=554055 RepID=A0A2P6VL84_9CHLO)

HSP 1 Score: 102 bits (253), Expect = 3.540e-24
Identity = 44/95 (46.32%), Postives = 72/95 (75.79%), Query Frame = 2 
Query:  281 LQMVESEVKEEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDE 565
            +++ E++ + +F++H+   + W ALC+AA+ +G+ EG LP   T E+LED +F++  H  L+++H+ EGALVCPETGR+FP++ G+PNL+ TEDE
Sbjct:   29 VEVQEADFEPDFLQHIFPRIQWAALCEAAAAMGV-EG-LPAEATEEMLEDDAFMKKFHHALLEVHLQEGALVCPETGRKFPVSAGIPNLLLTEDE 121
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: B5Y448_PHATC (Predicted protein n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B5Y448_PHATC)

HSP 1 Score: 102 bits (253), Expect = 4.690e-24
Identity = 43/84 (51.19%), Postives = 65/84 (77.38%), Query Frame = 2 
Query:  314 FIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDE 565
            F++ + +TLDW AL +AAS++G+P  +LPP LT +L ED  FLEALH VLM++H+++G L CP TGR FP+ +G+PN++  E++
Sbjct:   45 FVEGLLSTLDWSALIEAASQLGIP--TLPPVLTEDLAEDPEFLEALHHVLMNVHLIQGILTCPATGREFPVRDGIPNMVLEEED 126
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: A9S9D3_PHYPA (Predicted protein n=1 Tax=Physcomitrium patens TaxID=3218 RepID=A9S9D3_PHYPA)

HSP 1 Score: 100 bits (249), Expect = 1.400e-23
Identity = 44/96 (45.83%), Postives = 66/96 (68.75%), Query Frame = 2 
Query:  281 LQMVESEVKEEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDEV 568
            L+  ESE+  +F++H+   LDW A  +AA  VG+   ++P  + P +L+D  FL   H  L+++H+ EGAL+CPETGRRFP+ +GVPN++  EDEV
Sbjct:   29 LETKESELNADFLRHIFPKLDWKAFHEAAQSVGV--NNIPDQVEPVMLDDDEFLRKFHHALLEVHLEEGALICPETGRRFPVTKGVPNMLLNEDEV 122
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: A0A210QVU1_MIZYE (Multifunctional methyltransferase subunit TRM112-like protein n=1 Tax=Mizuhopecten yessoensis TaxID=6573 RepID=A0A210QVU1_MIZYE)

HSP 1 Score: 100 bits (250), Expect = 1.550e-23
Identity = 52/104 (50.00%), Postives = 69/104 (66.35%), Query Frame = 2 
Query:  278 PLQMVESEVK-------EEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDEV 568
            PL++V ++V+        EFI  M   +DW ALC+AA  VG  EG LP ALT +  ED   L+ +H VLM + V+EG LVCPETGR+FPI+ G+PN++  EDEV
Sbjct:   36 PLKIVPTQVEVKTVDFNPEFISRMLPKIDWPALCQAAQSVGHGEG-LPEALTEKYEEDEMLLKKVHHVLMQVEVLEGNLVCPETGRQFPISSGIPNMLLNEDEV 138
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: A0A1Y1INK2_KLENI (Uncharacterized protein n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1INK2_KLENI)

HSP 1 Score: 100 bits (248), Expect = 1.970e-23
Identity = 45/92 (48.91%), Postives = 65/92 (70.65%), Query Frame = 2 
Query:  293 ESEVKEEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDEV 568
            E++    F++H+   LDW AL +AA+ +G+ E  LP  + P  LED  FL   H VL+++H+ EGALVCPETGRRFP+++G+PN++  EDEV
Sbjct:   33 ETDFNAGFLRHIYPKLDWKALREAAASLGLEE--LPEQVEPSQLEDEDFLRRFHHVLLEIHLEEGALVCPETGRRFPVSKGIPNMLLNEDEV 122
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: I0Z8N2_COCSC (Trm112p-domain-containing protein n=2 Tax=Coccomyxa TaxID=41891 RepID=I0Z8N2_COCSC)

HSP 1 Score: 97.8 bits (242), Expect = 1.550e-22
Identity = 42/95 (44.21%), Postives = 68/95 (71.58%), Query Frame = 2 
Query:  281 LQMVESEVKEEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDE 565
            ++ V+++   +F++H+   L+W ALC+AA+ +G P   LP  ++ E+L+D  FL + H  L+++ + EGALVCPETGR+FP+ +GVPNL+  EDE
Sbjct:   29 VEQVDADYDPDFLRHIYPRLEWKALCEAAATMGAP--GLPEEVSEEMLQDDDFLRSFHHALLELVLEEGALVCPETGRQFPVHKGVPNLLLNEDE 121
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: UPI0006B0D279 (multifunctional methyltransferase subunit TRM112-like protein n=1 Tax=Limulus polyphemus TaxID=6850 RepID=UPI0006B0D279)

HSP 1 Score: 97.8 bits (242), Expect = 1.640e-22
Identity = 48/104 (46.15%), Postives = 66/104 (63.46%), Query Frame = 2 
Query:  278 PLQMVESEVKE-------EFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDEV 568
            PL++  +EVKE       EF+  M   LDW ALC+AA  +G     LP ++ P    D  FL+  H VL+++ V+EG LVCPETGR+FP+ EG+PN++  EDEV
Sbjct:   22 PLKICATEVKEVAVDFNPEFVTRMIPKLDWDALCQAAESLGCLN-DLPRSVNPNHENDEEFLKKAHHVLLEVEVIEGELVCPETGRKFPVNEGIPNMLLNEDEV 124
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BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Match: A0A0G4GWP5_VITBC (Uncharacterized protein n=2 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4GWP5_VITBC)

HSP 1 Score: 97.8 bits (242), Expect = 1.880e-22
Identity = 43/92 (46.74%), Postives = 65/92 (70.65%), Query Frame = 2 
Query:  293 ESEVKEEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDEV 568
            ESE + EFI+H+   LDW AL K A E+ +  G +PP+ + E  +D +FL A+H V+MD+H++EG LVCP   R +P+++G+PN++ T DEV
Sbjct:   39 ESEFRPEFIQHLLPKLDWEALYKTAQELNLSPG-VPPSYSEEDKKDENFLRAVHDVVMDVHILEGKLVCPNCNREYPVSKGIPNMLLTNDEV 129
The following BLAST results are available for this feature:
BLAST of mRNA_P-littoralis_Contig59.74.4 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G5K3_ECTSI3.210e-6088.57Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A836CHT2_9STRA9.880e-3255.34Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A2P6VL84_9CHLO3.540e-2446.32Multifunctional methyltransferase subunit TRM112 A... [more]
B5Y448_PHATC4.690e-2451.19Predicted protein n=1 Tax=Phaeodactylum tricornutu... [more]
A9S9D3_PHYPA1.400e-2345.83Predicted protein n=1 Tax=Physcomitrium patens Tax... [more]
A0A210QVU1_MIZYE1.550e-2350.00Multifunctional methyltransferase subunit TRM112-l... [more]
A0A1Y1INK2_KLENI1.970e-2348.91Uncharacterized protein n=1 Tax=Klebsormidium nite... [more]
I0Z8N2_COCSC1.550e-2244.21Trm112p-domain-containing protein n=2 Tax=Coccomyx... [more]
UPI0006B0D2791.640e-2246.15multifunctional methyltransferase subunit TRM112-l... [more]
A0A0G4GWP5_VITBC1.880e-2246.74Uncharacterized protein n=2 Tax=Vitrella brassicaf... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-littoralis_Contig59contigP-littoralis_Contig59:435210..437472 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Pylaiella littoralis U1_48 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7G5K3
Preferred nameTRMT112
PFAMsTrm112p
Model size668
Max annot lvl2759|Eukaryota
KEGG koko:K15448
Hectar predicted targeting categoryother localisation
GOsGO:0000154,GO:0001510,GO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0006139,GO:0006364,GO:0006396,GO:0006399,GO:0006400,GO:0006464,GO:0006479,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0008168,GO:0008213,GO:0008276,GO:0009451,GO:0009893,GO:0009987,GO:0010467,GO:0010468,GO:0010604,GO:0010628,GO:0016070,GO:0016072,GO:0016740,GO:0016741,GO:0018364,GO:0019219,GO:0019222,GO:0019538,GO:0022613,GO:0030488,GO:0031167,GO:0031323,GO:0031325,GO:0031974,GO:0031981,GO:0032259,GO:0032991,GO:0034470,GO:0034641,GO:0034660,GO:0036211,GO:0036265,GO:0042254,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043414,GO:0044085,GO:0044087,GO:0044089,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044464,GO:0045935,GO:0046483,GO:0046982,GO:0046983,GO:0048471,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051252,GO:0051254,GO:0060255,GO:0065007,GO:0070013,GO:0070475,GO:0070476,GO:0071704,GO:0071840,GO:0080090,GO:0090069,GO:0090070,GO:0090304,GO:0140096,GO:1901360,GO:1901564,GO:2000232,GO:2000234
Exons3
Evalue1.3e-62
EggNOG OGsKOG1088@1|root,KOG1088@2759|Eukaryota
Ec32 ortholog descriptionUncharacterised protein family UPF0434/Trm112
Ec32 orthologEc-22_000960.1
Descriptionpeptidyl-glutamine methylation
Cds size426
COG categoryS
BRITEko00000,ko03012,ko03016
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791216.619347-UTR-P-littoralis_Contig59:435209..4353571680791216.619347-UTR-P-littoralis_Contig59:435209..435357Pylaiella littoralis U1_48UTRP-littoralis_Contig59 435210..435357 +
1680791216.6596518-UTR-P-littoralis_Contig59:437378..4374721680791216.6596518-UTR-P-littoralis_Contig59:437378..437472Pylaiella littoralis U1_48UTRP-littoralis_Contig59 437379..437472 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791216.6331646-CDS-P-littoralis_Contig59:435357..4355821680791216.6331646-CDS-P-littoralis_Contig59:435357..435582Pylaiella littoralis U1_48CDSP-littoralis_Contig59 435358..435582 +
1680791216.642212-CDS-P-littoralis_Contig59:436291..4364671680791216.642212-CDS-P-littoralis_Contig59:436291..436467Pylaiella littoralis U1_48CDSP-littoralis_Contig59 436292..436467 +
1680791216.6507332-CDS-P-littoralis_Contig59:437353..4373781680791216.6507332-CDS-P-littoralis_Contig59:437353..437378Pylaiella littoralis U1_48CDSP-littoralis_Contig59 437354..437378 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-littoralis_Contig59.74.4prot_P-littoralis_Contig59.74.4Pylaiella littoralis U1_48polypeptideP-littoralis_Contig59 435358..437378 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-littoralis_Contig59.74.4

>prot_P-littoralis_Contig59.74.4 ID=prot_P-littoralis_Contig59.74.4|Name=mRNA_P-littoralis_Contig59.74.4|organism=Pylaiella littoralis U1_48|type=polypeptide|length=142bp
MWRPREKSRRNLMKWCCCYDITEYIAKAALFAFLCPPRLASSAPLQMVES
EVKEEFIKHMANTLDWGALCKAASEVGMPEGSLPPALTPELLEDSSFLEA
LHRVLMDMHVVEGALVCPETGRRFPIAEGVPNLMCTEDEVR*
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mRNA from alignment at P-littoralis_Contig59:435210..437472+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-littoralis_Contig59.74.4 ID=mRNA_P-littoralis_Contig59.74.4|Name=mRNA_P-littoralis_Contig59.74.4|organism=Pylaiella littoralis U1_48|type=mRNA|length=2263bp|location=Sequence derived from alignment at P-littoralis_Contig59:435210..437472+ (Pylaiella littoralis U1_48)
GTTTCTCGTTTCTCTTCGTGAGCTTTCATGAAAAAAGACAGCAGACAGAC
AGATAAACAGACAGACAGGCAGGCATTGTTTGGCTGTTCTTACGCTGAGA
TTTGATGTGTGATCGCTTGTCTGTGGCGACCAAGTCTGTCCTGCAGGCAT
GTGGCGGCCTCGAGAAAAGTCACGAAGAAATCTCATGAAATGGTGCTGCT
GCTACGATATAACCGAATACATCGCAAAAGCTGCCCTTTTTGCGTTTCTG
TGCCCCCCTCGGTTGGCGTCGTCGGCGCCTCTCCAGATGGTGGAATCGGA
AGTCAAAGAAGAATTTATCAAGCACATGGCAAACACTCTGGATTGGGGAG
CTCTCTGCAAAGCAGCATCCGAGGTACGTACTACGTACCCGCATTTTCGT
TTCATCGGGTGCTCACTTCTGCTGCTAGCAATCCGCACCATCCATCAACC
GACGCATCACTCCTTCCCTCGATTACTCAACACCCAATTAAGATACAGTT
TCGAAGTCCAAGCCTGTGTTGTGTCCCTTGGAAAAGGTGTCTCCTGAACT
ATATTCAAAATAGACATTTATATATATTTAGAAGAACTCTCTCGTACTTT
CCTGAAAAATCGAGCCTACCTCCGTTTCTGACGCATTCGCTGTTTGCTGG
GTGTCCTGTAACACATCGCTATCGACGAACCGACCCATCCATTCAACCCA
TCCGTCGATCACTAAATGCCTCTTGATTTTGCTCATGTTGGCCATGTTTC
ATGCCTGCTGCTCGTTTGCTTACTTCCTTTCAACAACTCAAAACGAACAA
CCCAAATCACCAATAATGTTGGCTTGATCGCCTTTTGTGTGTTTTGCCTG
CTTGCTCTCGAACACGTGAACCAAAAATCAAGTTCATAAAGAAACAAGCA
AATAGGCAAACAAACAAACAAAACAAACAAACAACGAGCAGACAGACACA
CAACCAACCAAACAAGCAAACAAACAAACAAATCTAGCAAGCAAACACAC
AAGCAAAAAATGAAGCACAACAAACAACGCTGTCTCATGATACGCATTCG
TGGACCTACTCAACAAAACGAACGGAAACAAGGTCGGCATGCCCGAAGGT
TCTTTGCCCCCCGCCCTGACCCCGGAGCTGCTAGAGGACTCCTCCTTCCT
CGAGGCCTTGCATCGCGTCTTGATGGACATGCACGTTGTGGAGGGCGCCC
TCGTTTGTCCGGAGACGGGACGTAGGTTTCCCATCGCGGAAGGCGTGCCG
AACCTCATGTGAGTTTTTGGAGGTTGTTCGTCACCTCCCTTCGCTGAAAA
TTCCACGTAGTCACACTTGGTCTATAACCACCGCGGGTGGTACCTTTGAG
AGAGTGCGCATCATTGGTAGGGAACGGCTTGTAACATTTGTAGGGAAATG
TGAGTGTTGTGGGCCCACCCTTGAGTACGGTAGCTTGACAAAGAAGCGGT
TTTAACCATCGATGGTAAGAGTTGTGATTGTACGTCAGTACGTGTTGGTT
CGTGTTGGCGCCAATGTTTTTCTGATGTGGAGATGGGCTTCAGATGGCAA
TCCTTGCGAGTAGTCTTTGTTTTTGTTTGGTGTGATAAGCATGGTGTTTG
TGTGCCGTACGGATGCATTCATGCGCGCATCGCATGATCGAATTTTTCTC
AAGCACGAGTACTGTTGACGTCCGTTTTGTCCTGCCTGCTAAAAAAAGTG
CGAGCTACTACTTTATGACATCGCTTATTGTTTTGCTTGTTTATCCTCTC
GTATCATCCGCTTCTTGTGACATGCTAGCTCATAAATGCAGACGCACCTG
GCACACAACTGTGTAACACGCAAAGACGTATCGTACCCTTCGAAGTAGAA
TTTTGGGGACAAAGAAACGCAAAATGGATTACGCACACAGAGGCGAGATA
AAAGTTCTCGGACAAGGCTTTACGTATGCGTGCGGTGGCGAAGATCGCCG
TCTGTGGATTGCACCATCAGGTGCACGCCAAGAAGAAGACAACAGTGCCA
TCAACGAAAACGTTTACACGATAGTGCGTAGCACCTGCATGCTACATGCT
ACCGCTTTTGCCGCAGCACCGTCCTATTTTTAATTTCTACCTGGTACTTT
TTATCGTGCGTCTTTTTTTCCCGTTTTTTTTTTTTTTTTGGCAGGTGCAC
CGAGGACGAGGTGCGATAAGCTGAGGACCGAAAAAACATAGCGGTAGCCG
TACACGCCTTTTTTTACTCTGTCCGAAAAAAAAAGCGAGGCAGCGGACGT
GATATGCGCGTGC
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Coding sequence (CDS) from alignment at P-littoralis_Contig59:435210..437472+

>mRNA_P-littoralis_Contig59.74.4 ID=mRNA_P-littoralis_Contig59.74.4|Name=mRNA_P-littoralis_Contig59.74.4|organism=Pylaiella littoralis U1_48|type=CDS|length=426bp|location=Sequence derived from alignment at P-littoralis_Contig59:435210..437472+ (Pylaiella littoralis U1_48)
ATGTGGCGGCCTCGAGAAAAGTCACGAAGAAATCTCATGAAATGGTGCTG
CTGCTACGATATAACCGAATACATCGCAAAAGCTGCCCTTTTTGCGTTTC
TGTGCCCCCCTCGGTTGGCGTCGTCGGCGCCTCTCCAGATGGTGGAATCG
GAAGTCAAAGAAGAATTTATCAAGCACATGGCAAACACTCTGGATTGGGG
AGCTCTCTGCAAAGCAGCATCCGAGGTCGGCATGCCCGAAGGTTCTTTGC
CCCCCGCCCTGACCCCGGAGCTGCTAGAGGACTCCTCCTTCCTCGAGGCC
TTGCATCGCGTCTTGATGGACATGCACGTTGTGGAGGGCGCCCTCGTTTG
TCCGGAGACGGGACGTAGGTTTCCCATCGCGGAAGGCGTGCCGAACCTCA
TGTGCACCGAGGACGAGGTGCGATAA
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