mRNA_P-littoralis_Contig21.182.11 (mRNA) Pylaiella littoralis U1_48

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-littoralis_Contig21.182.11
Unique NamemRNA_P-littoralis_Contig21.182.11
TypemRNA
OrganismPylaiella littoralis U1_48 (Pylaiella littoralis U1_48 (Mung))
Homology
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BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Match: D7FNK3_ECTSI (Fanconi-associated nuclease n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNK3_ECTSI)

HSP 1 Score: 236 bits (603), Expect = 1.860e-70
Identity = 143/228 (62.72%), Postives = 164/228 (71.93%), Query Frame = 2 
Query:  293 QTPFQDAPLDLDFCPLFYLSRRVAVDARLAEIRAASETKLVSLVGNAWLANAHTVCRGLNWERSSVRHLQAIAAGLGGPAVAAVLAALCLNYKHFGGGLPDLLLMRAVRTGRDTRAAAEEGDTSAKESESAGVEAAPXXXXXAPSAEAEVST-----PERLASWLGMESGAVFDADEKNGGGSQPTGR---GGSGRGRSGGANNRAPPPPFEANDMNGLRVETWLLEV 952
            QTPFQDAPLDLDFCPLFYL+RR AVD RLAE+RAAS  +LV+LVG+AWLANA TVCRGLNW+RSSVRHLQAIAAGLGGPA+AA+LAALCLN+KHFGGGLPDLLLMRAVRT  DT AAA       ++ + A  +AA      A  AE+EVST     P    SWLG++S   FD D   GG  +P      GG+G+ R  G   R     F+A DM GLR+ETWLLEV
Sbjct:   77 QTPFQDAPLDLDFCPLFYLNRRTAVDTRLAELRAASAAELVALVGDAWLANAGTVCRGLNWDRSSVRHLQAIAAGLGGPAMAAILAALCLNHKHFGGGLPDLLLMRAVRT--DTNAAAVAAAGEGRDGKIAKGKAAAEAAPPAFEAESEVSTVRFSGPAAFVSWLGIDSEVAFDVD---GGDERPXXXXXDGGNGK-RGKGVPMRXXXXXFKAADMAGLRLETWLLEV 298
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BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Match: A0A6H5L3R1_9PHAE (Fanconi-associated nuclease n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L3R1_9PHAE)

HSP 1 Score: 245 bits (626), Expect = 8.580e-68
Identity = 140/220 (63.64%), Postives = 161/220 (73.18%), Query Frame = 2 
Query:  293 QTPFQDAPLDLDFCPLFYLSRRVAVDARLAEIRAASETKLVSLVGNAWLANAHTVCRGLNWERSSVRHLQAIAAGLGGPAVAAVLAALCLNYKHFGGGLPDLLLMRAVRTGRDTRAAAEEGDTSAKESESAGVEAAPXXXXXAPSAEAEVSTPERLASWLGMESGAVFDADEKNGGGSQPTGRGGSGRGRSGGANNRAPPPPFEANDMNGLRVETWLLEV 952
            QTPFQDAPLDLDFCPLFYL+RR AVDARLAE+RAAS  +LV+LVG+AWLANA TVCRGLNW++SSVRHLQAIAAGLGGPA+A++ AALCLN+KHFGGGLPDLLLMRAVRT  DT AA        ++ + A  +AA      A    +EVSTP    SWLG++S   FD D  N     P    GSG+ R  G   R  PPPF+A DM GLR+ETWLLEV
Sbjct: 1238 QTPFQDAPLDLDFCPLFYLNRRTAVDARLAELRAASAAELVALVGDAWLANAGTVCRGLNWDKSSVRHLQAIAAGLGGPAMASISAALCLNHKHFGGGLPDLLLMRAVRT--DTNAAVVAATGEGRDGKIAEGKAAAEAEPPAFEVVSEVSTPAAFVSWLGIDSEVAFDVDGGNERPPPPPTDRGSGK-RDKGVPKRPSPPPFKAADMAGLRLETWLLEV 1454
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BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Match: L1J0F1_GUITC (Fanconi-associated nuclease (Fragment) n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1J0F1_GUITC)

HSP 1 Score: 105 bits (261), Expect = 7.050e-23
Identity = 49/108 (45.37%), Postives = 71/108 (65.74%), Query Frame = 2 
Query:  296 TPFQDAPLDLDFCPLFYLSRRVAVDARLAEIRAASETKLVSLVGNAWLANAHTVCRGLNWERSSVRHLQAIAAGLGGPAVAAVLAALCLNYKHFGGGLPDLLLMRAVR 619
            TPFQDAP+DLDF P FY  R   + AR+A++   S+ +L+  +   W  +  T+CRG +W   S+  LQ +A  LGG  ++A+  ALC+NY+HF GG+PDL+L+R  R
Sbjct:   52 TPFQDAPIDLDFHPFFYKRREALILARIADLELWSDQQLLLELRERWQQSFGTLCRGASWGSCSLSFLQLVALCLGGRRISAICRALCINYRHFSGGMPDLVLLRGRR 159
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BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Match: A0A482R5S3_9EURY (VRR-NUC domain-containing protein n=1 Tax=Euryarchaeota archaeon TaxID=2026739 RepID=A0A482R5S3_9EURY)

HSP 1 Score: 90.5 bits (223), Expect = 1.250e-15
Identity = 49/113 (43.36%), Postives = 67/113 (59.29%), Query Frame = 2 
Query:  296 TPFQDAPLDLDFCPL-FYLSRRVAVDARLAEIRA-----ASETKLVSLVGNAWLANAHTVCRGLNWERSSVRHLQAIAAGLGGPAVAAVLAALCLNYKHFGGGLPDLLLMRAV 616
            TP+ DAPLDLD CPL FY  RR A+     E+ A     +    L++ +G  W  +   V RG+ W   S++ LQ +A G+GGPA A ++ A+C +YKH   GLPDLLL R +
Sbjct:  464 TPYTDAPLDLD-CPLLFYAHRREAIVRLTTELAACGADDSGRAALIARIGGTWRKHKGCVARGMRWGSFSLQLLQLLAVGMGGPACAMLVDAMCFDYKHLASGLPDLLLWRVL 575
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BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Match: A0A7S3HLV0_9STRA (Fanconi-associated nuclease n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HLV0_9STRA)

HSP 1 Score: 84.0 bits (206), Expect = 1.410e-13
Identity = 49/108 (45.37%), Postives = 69/108 (63.89%), Query Frame = 2 
Query:  296 TPFQDAPLDLDFCPLFYLSRRVAVDARLAEIRAASETKLVSLVGNAWLANAHTVCRGLNWERSSVRHLQAIAAGLGGPAVAAVLAALCLNYKHFGGGLPDLLLMRAVR 619
            TPFQDAPLDL     FY +RRVA+  R+A+I + S  +L++ +G  +  +    CRG++W   ++  LQ +A  +G   VAAV  AL +NYKHF GG PDL+L+R  R
Sbjct:  260 TPFQDAPLDLGHRS-FYRTRRVAIQKRIAQIASFSTPQLIAELGVVYRKHYGENCRGVHWG-VTLPLLQLMAVCMGSATVAAVCNALSVNYKHFTGGAPDLMLVRVQR 365
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BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Match: A0A836CMA8_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CMA8_9STRA)

HSP 1 Score: 61.2 bits (147), Expect = 1.100e-8
Identity = 29/49 (59.18%), Postives = 37/49 (75.51%), Query Frame = 2 
Query:  461 RGLNWERSSVRHLQAIAAGLGGPAVAAVLAALCLNYKHFGGGLPDLLLM 607
            R L W R S+  L AIAAG+GG  +A++L ALCL+ +H GGGLPDLLL+
Sbjct:    3 RWLRWGRLSLPQLMAIAAGIGGAGLASLLTALCLDSRHLGGGLPDLLLL 51
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BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Match: A0A7S4AK23_9STRA (Fanconi-associated nuclease n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4AK23_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 1.740e-6
Identity = 42/124 (33.87%), Postives = 60/124 (48.39%), Query Frame = 2 
Query:  296 TPFQDAPLDLDF-----------CPLFYLSRRVAVDARLAEIRAASETKLVSLV-----GNAWLANA-HTVCRGLNWERSSVRHLQAIAAGLGGPAVAAVLAALCLNYKHFGGGLPDLLLMRAV 616
            TP+Q AP DL             C   Y  R+ ++D  L ++ +     L S V        W  N+ H     L  +   VR L  +AAGLGG  +A++   L  +Y+H+ GGLPDLLL+RA+
Sbjct:  670 TPYQGAPFDLHVGAEQIDSTGLGCGGIYSRRKSSIDDFLGKLSSLDGEGLSSFVYDSINSRVWYTNSLHRPDLVLESDLKQVRTLSMVAAGLGGKLLASIFRCLFFDYRHYSGGLPDLLLVRAL 793
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BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Match: A0A7S4R0Q4_9STRA (Fanconi-associated nuclease n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4R0Q4_9STRA)

HSP 1 Score: 58.5 bits (140), Expect = 2.370e-5
Identity = 43/143 (30.07%), Postives = 64/143 (44.76%), Query Frame = 2 
Query:  296 TPFQDAPLDLDFCPL----------------------------FYLSRRVAVDARLAEIRAASETKLVSLVGNAWLANAHTVCRG--------LNWERSSVRHLQAIAAGLGGPAVAAVLAALCLNYKHFGGGLPDLLLMRAV 616
            TP+Q APLDL    L                            F+  RR  ++  L ++   S+  L  LV  +  +   ++ +         L+ +   VR L  IAAGLGG  ++A+   LC +Y+H+ GG+PDLLL RAV
Sbjct: 1328 TPYQGAPLDLHVGQLMCGRITTKESLKIYDSQEIRLTCAPSRSFFERRRSKIEQYLRKVECMSQQDLCDLVYESVTSQICSLEKDSISTNDTFLSRDLKEVRTLSMIAAGLGGKLLSAIFRCLCFDYRHYSGGMPDLLLARAV 1470
The following BLAST results are available for this feature:
BLAST of mRNA_P-littoralis_Contig21.182.11 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
D7FNK3_ECTSI1.860e-7062.72Fanconi-associated nuclease n=1 Tax=Ectocarpus sil... [more]
A0A6H5L3R1_9PHAE8.580e-6863.64Fanconi-associated nuclease n=1 Tax=Ectocarpus sp.... [more]
L1J0F1_GUITC7.050e-2345.37Fanconi-associated nuclease (Fragment) n=1 Tax=Gui... [more]
A0A482R5S3_9EURY1.250e-1543.36VRR-NUC domain-containing protein n=1 Tax=Euryarch... [more]
A0A7S3HLV0_9STRA1.410e-1345.37Fanconi-associated nuclease n=1 Tax=Spumella elong... [more]
A0A836CMA8_9STRA1.100e-859.18Uncharacterized protein (Fragment) n=1 Tax=Tribone... [more]
A0A7S4AK23_9STRA1.740e-633.87Fanconi-associated nuclease n=1 Tax=Pseudo-nitzsch... [more]
A0A7S4R0Q4_9STRA2.370e-530.07Fanconi-associated nuclease n=2 Tax=Ditylum bright... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-littoralis_Contig21contigP-littoralis_Contig21:673178..675965 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Pylaiella littoralis U1_48 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FNK3
PFAMsVRR_NUC
Model size1388
Max annot lvl2759|Eukaryota
KEGG koko:K15363
KEGG Pathwayko03460,map03460
Hectar predicted targeting categoryother localisation
Exons3
Evalue2.72e-05
EggNOG OGsKOG2143@1|root,KOG2143@2759|Eukaryota
EC3.1.4.1
Descriptionphosphodiesterase I activity
Cds size705
COG categoryS
BRITEko00000,ko00001,ko01000,ko03400
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790017.0120034-UTR-P-littoralis_Contig21:673177..6732561680790017.0120034-UTR-P-littoralis_Contig21:673177..673256Pylaiella littoralis U1_48UTRP-littoralis_Contig21 673178..673256 -
1680790017.0448017-UTR-P-littoralis_Contig21:674770..6748721680790017.0448017-UTR-P-littoralis_Contig21:674770..674872Pylaiella littoralis U1_48UTRP-littoralis_Contig21 674771..674872 -
1680790017.058177-UTR-P-littoralis_Contig21:675463..6759651680790017.058177-UTR-P-littoralis_Contig21:675463..675965Pylaiella littoralis U1_48UTRP-littoralis_Contig21 675464..675965 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790017.0252938-CDS-P-littoralis_Contig21:673256..6738171680790017.0252938-CDS-P-littoralis_Contig21:673256..673817Pylaiella littoralis U1_48CDSP-littoralis_Contig21 673257..673817 -
1680790017.0349092-CDS-P-littoralis_Contig21:674626..6747701680790017.0349092-CDS-P-littoralis_Contig21:674626..674770Pylaiella littoralis U1_48CDSP-littoralis_Contig21 674627..674770 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-littoralis_Contig21.182.11prot_P-littoralis_Contig21.182.11Pylaiella littoralis U1_48polypeptideP-littoralis_Contig21 673257..674770 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-littoralis_Contig21.182.11

>prot_P-littoralis_Contig21.182.11 ID=prot_P-littoralis_Contig21.182.11|Name=mRNA_P-littoralis_Contig21.182.11|organism=Pylaiella littoralis U1_48|type=polypeptide|length=235bp
MRAVRTGRDTRAAAEEGDTSAKESESAGVEAAPAPAPVAPSAEAEVSTPE
RLASWLGMESGAVFDADEKNGGGSQPTGRGGSGRGRSGGANNRAPPPPFE
ANDMNGLRVETWLLEVSLFFFFCAKQHIYMQLESGLRSVFEFVCFLRLLQ
ISSVARRVGVSRKIRDKVPSNLWRHHACNGIRVCCSGRARGTFFLGRRRY
SSLSLSCCCRYVACARAPQIHTTAASLSMLPFEG*
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mRNA from alignment at P-littoralis_Contig21:673178..675965-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-littoralis_Contig21.182.11 ID=mRNA_P-littoralis_Contig21.182.11|Name=mRNA_P-littoralis_Contig21.182.11|organism=Pylaiella littoralis U1_48|type=mRNA|length=2788bp|location=Sequence derived from alignment at P-littoralis_Contig21:673178..675965- (Pylaiella littoralis U1_48)
GTGCCAATGTGGTAACTTCTAGCCCCGTGTGCTCCGTGTTGTCTGATATC
TTCTATTGCCGTTGCCAGGTAGGTAGGTAATTGATCTTGACATACTGACA
TCAACGTGTTTAGCACACACGAAAGTGCCACGCACATACGGTAGTACTCC
GAAGAAGTACGTAGCACTTCCTCCGCCCCGCAGCCATTCCTTTCACGCGT
GCACGCAATTGCGTGTCGTTACGTTCTTGTACCGCCATCCGTCCTGCCCG
TCCTCCCGCAAAACAAACAAACAAACAAACAAACATTTTTGACAGACGCC
GTTTCAAGACGCCCCACTGGATCTGGACTTTTGCCCCCTCTTCTACCTAA
GCCGGCGCGTGGCTGTGGACGCGCGCTTGGCGGAAATCCGGGCCGCTTCT
GAGACGAAGCTGGTCTCCCTGGTCGGAAACGCCTGGCTGGCCAATGCCCA
CACGGTGTGCCGTGGCCTGAATTGGGAACGGTCGTCCGTACGCCATCTTC
AGGCGCGTGGGGGTGTTTCATTGCGAGATCGGGGTCGGGGCATGTCCTCG
CTTTGCTGCAGAAGTAGCTGTCTCGGATAACGCTCCGTATTTGTTTTTCG
ACCATATCTCGCATACCCTTCCCTTCCCTTCCCTTCCCTTCCCTCCCCTT
GCCGTTCGTCCCGTCTGTGCACGTTTTGCGGTGTGATCATGGTGGGTGGG
TGCATGGGTGGGTGAGAGCGAGGCAGCAGAAGTGCGGTTCAGCAGGAGGA
GGCCCGCCTCCTATTTTTTGCCTCTTGACTCGTGCTTGCGCTCCTCTGCA
GGGCCATTTTTTCCGGCGTTCCGCGACAGATGAGACATTAGTCTGCACTG
TACGTCGTTGAGACATTAGTCTGCACTGTACTTCGAGTATTCTTCTCTCA
GCTTCTCTCTCTCTTGCTTGTTGTGCCGCGCGTAAATGTCGGTCTCTCTC
CCTCCGGAAACGCCCCTTATCTTTATCGAGTGAAGTTCGCCACCACAGCT
GTGCTAGAGTGTCACCCACCAACCACTCCCGTTGTCTCTGTTGTTGTTGT
TGTTGTGGTGGTGGTGGTGTCGGTTTGTTCCCCCTCGATGTAGGCGATCG
CGGCGGGGCTCGGCGGGCCCGCGGTGGCCGCAGTTTTGGCTGCGCTTTGC
CTCAACTACAAGCACTTCGGGGGCGGGTTACCGGACCTGCTTCTGATGCG
CGCGGTGCGGACGGGCCGGGACACCAGAGCGGCCGCAGAAGAAGGCGATA
CCTCTGCGAAGGAAAGTGAAAGTGCGGGAGTAGAGGCGGCGCCAGCACCG
GCTCCCGTCGCCCCTAGCGCTGAGGCTGAGGTTTCTACGGTGCGTGCCCC
ACGGCAAAAGTTATGTTTTTCAGGAGAGGGGGAGGTAATTGGTTGTGTGC
TCTTATCTTGTTGCGCCTGTGTATTCTTTTTTTGGCTTGCGCGCCGAACG
AAGTTCTTGCGTGTCGCTTGAGTGAGGGGGGGTCAGTCACCGTTCTATTC
ACTCTACCTCGGAGCCATGATACTGTTGTGCGTGCTGTCGAGCGTTAGCC
CGAGGATGGTTCAGACGAACTTGTCGCCATCATCGTTTAGAGCAAATAGG
AAAGGCCGACAAGACGTACACACTACTACCGTTCGTACCCGGCCGGCGCA
CAGACCACCCGCTGCCGCGTACTGAGGTCCATCCCGCAAATGTCGCACAA
TTGCCGTGACAAAACAACGTTCCACCGTAAAGTGGGCAGGCTGCTGTCCT
ATTCCGGGCTGTACACAACCTCTTTCCTCCTTCCCTCCCTCCCTCCCTCC
CTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCTCTTTCTCCTC
ATCTACAGCATCTGACTTCGTCACTGCTTGCCTGCTTGCCTGCTTGCCTG
CTTGCCTGCTTGCCTGCTTGCCTGCTTGCCTGCTTGCCTGCTTGCCTGCC
TCCTTGAGTCCCATCCATCTCATTTTCGGACCACAACCTTCGCAACCTCT
TCTTCTCCACTCGGATAGTCTTTTCTCATAAGTACATATGCTTATGTTCT
CACTCGGAGACCGACGGTGTTATTGCTTTTTCTCACCCCCCCCCCCACCC
CTACCGCGGGCGCGCGCTCACCCCATAACGCCGCTGAAATCCTCTCAGCC
GGAAAGATTAGCCTCTTGGCTGGGAATGGAGTCGGGCGCTGTATTTGACG
CCGACGAAAAAAACGGGGGGGGATCACAACCCACCGGCAGAGGCGGGAGT
GGCAGGGGTAGGAGCGGCGGCGCCAATAATAGAGCGCCACCTCCCCCGTT
CGAGGCAAATGACATGAACGGGTTACGGGTGGAAACGTGGCTGTTGGAAG
TGAGTCTCTTTTTTTTTTTTTGCGCAAAACAGCATATATACATGCAGCTC
GAGTCGGGTCTGCGAAGTGTCTTCGAGTTTGTTTGTTTTTTACGCTTGCT
GCAGATCTCCTCTGTCGCCCGCCGTGTGGGTGTGTCGAGAAAGATACGTG
ATAAGGTCCCCTCCAATCTGTGGAGGCACCATGCGTGCAACGGGATTCGA
GTCTGTTGTTCTGGCCGGGCACGTGGGACATTTTTCCTTGGACGTCGGCG
TTACTCCTCTCTCTCCCTCTCCTGTTGTTGTCGCTATGTTGCCTGTGCTC
GTGCGCCGCAAATACATACCACGGCGGCGTCGCTCTCGATGCTCCCTTTC
GAAGGTTAAGGGGCCCAGCGACCGCCTGTCCGACCGGCAGACGGCTTGGT
TGCGCATCTTGGGCCGCGGGCGCGCCCGCGTAGGGGTG
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Coding sequence (CDS) from alignment at P-littoralis_Contig21:673178..675965-

>mRNA_P-littoralis_Contig21.182.11 ID=mRNA_P-littoralis_Contig21.182.11|Name=mRNA_P-littoralis_Contig21.182.11|organism=Pylaiella littoralis U1_48|type=CDS|length=705bp|location=Sequence derived from alignment at P-littoralis_Contig21:673178..675965- (Pylaiella littoralis U1_48)
ATGCGCGCGGTGCGGACGGGCCGGGACACCAGAGCGGCCGCAGAAGAAGG
CGATACCTCTGCGAAGGAAAGTGAAAGTGCGGGAGTAGAGGCGGCGCCAG
CACCGGCTCCCGTCGCCCCTAGCGCTGAGGCTGAGGTTTCTACGCCGGAA
AGATTAGCCTCTTGGCTGGGAATGGAGTCGGGCGCTGTATTTGACGCCGA
CGAAAAAAACGGGGGGGGATCACAACCCACCGGCAGAGGCGGGAGTGGCA
GGGGTAGGAGCGGCGGCGCCAATAATAGAGCGCCACCTCCCCCGTTCGAG
GCAAATGACATGAACGGGTTACGGGTGGAAACGTGGCTGTTGGAAGTGAG
TCTCTTTTTTTTTTTTTGCGCAAAACAGCATATATACATGCAGCTCGAGT
CGGGTCTGCGAAGTGTCTTCGAGTTTGTTTGTTTTTTACGCTTGCTGCAG
ATCTCCTCTGTCGCCCGCCGTGTGGGTGTGTCGAGAAAGATACGTGATAA
GGTCCCCTCCAATCTGTGGAGGCACCATGCGTGCAACGGGATTCGAGTCT
GTTGTTCTGGCCGGGCACGTGGGACATTTTTCCTTGGACGTCGGCGTTAC
TCCTCTCTCTCCCTCTCCTGTTGTTGTCGCTATGTTGCCTGTGCTCGTGC
GCCGCAAATACATACCACGGCGGCGTCGCTCTCGATGCTCCCTTTCGAAG
GTTAA
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