mRNA_P-littoralis_Contig193.63.1 (mRNA) Pylaiella littoralis U1_48

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-littoralis_Contig193.63.1
Unique NamemRNA_P-littoralis_Contig193.63.1
TypemRNA
OrganismPylaiella littoralis U1_48 (Pylaiella littoralis U1_48 (Mung))
Homology
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BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A6H5KT20_9PHAE (FtsJ domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KT20_9PHAE)

HSP 1 Score: 251 bits (641), Expect = 2.350e-72
Identity = 201/446 (45.07%), Postives = 231/446 (51.79%), Query Frame = 2 
Query:   35 GRVETAERKGRRTGVVVAVDLLDLEVDLPGVEAIRGDFRDVSVELKILASLAARERXXXXXXXXCPTTEAT----MKSGEETSVVDETTRASLGRNSDGGGDGGVENIGGFNKGKEEEGNVQKDFNGERVFLPRGTSASRALLPRPEQPSTDPLEELFLAATSTPSVAAEEPTATPAAPAAQDIKPSSLVVGGVRRGRVSRDENGANRSPPSAPSSSTPPGSGRRRRANVVLSDMAPSFSGDRDTDQARVAGLVLDALAACLGDEATRLREQPQRCGAGAGGSDPQDREARGRSTGAAAYPCR-ADGDEAMSSTRSSGRILPGKGGQGSVTDVPGISFPGPGEMNSSSSSRRSDVRGRLTVPCSENERNVGGGGDEIEEEGSDGTGLLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGFLLGGRK 1357
            G +   +R+G    VVVAVDLLD+E DLPGV+AIRGDFRD  VE +I + L+ARER         P  +      ++  E T+      RA L R+     D  + +  G+     ++G+       E  FL RGTSASR LLPRPEQPSTD LE LFLA                  PA                                              RA+VVLSDMAPSFSGDRDTDQ RVA LVLDALAACLGDEA+R R +P R GAG  G +  D    G  +GA       A G++A    R  G  +    G    +DV   S  G       SSSR+  V GR                DE      +G GLLARGGTFLGKFFAGRDEREVKEEAERLFE VKVVKPPASRSGSGE YLLATGFLLGGRK
Sbjct:  132 GVLSAGDRRGGEA-VVVAVDLLDMEADLPGVQAIRGDFRDAEVESRIFSLLSARERLQPAPGNERPKLDEQRVLPLQPPEVTAAESGKERADLRRD-----DTKLRSSDGYGV---DDGSASDGVGEEDAFLLRGTSASRTLLPRPEQPSTDSLEHLFLAGGGNRD------------PATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTRASVVLSDMAPSFSGDRDTDQGRVAALVLDALAACLGDEASR-RSEPPRKGAGERGRNSVDPLGGGEGSGAVRLGGDVAGGEKAARMRREEGNEVGATTGGTLSSDVGVSSNGGTAAETPVSSSRKHAVGGR----------------DEWGGIEGEGPGLLARGGTFLGKFFAGRDEREVKEEAERLFERVKVVKPPASRSGSGEMYLLATGFLLGGRK 539
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BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A6L5Z4E8_9RHOB (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Halovulum marinum TaxID=2662447 RepID=A0A6L5Z4E8_9RHOB)

HSP 1 Score: 58.2 bits (139), Expect = 1.050e-5
Identity = 32/60 (53.33%), Postives = 41/60 (68.33%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGFLLGGRK 1357
            +L  GGTF+ K  AG  E E+ ++ +R +  VK VKPPASRS S EK++LATGF   GRK
Sbjct:  180 ILVPGGTFVAKVLAGGAEGELLQQLKRDYASVKHVKPPASRSDSSEKFVLATGFR--GRK 237
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BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A2S7K0A4_9PROT (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Marinicaulis flavus TaxID=2058213 RepID=A0A2S7K0A4_9PROT)

HSP 1 Score: 58.2 bits (139), Expect = 1.260e-5
Identity = 30/54 (55.56%), Postives = 38/54 (70.37%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGF 1339
            +LA GG F+ K FAG  E ++    ++ FE VK VKP ASRSGS EKY++ATGF
Sbjct:  215 VLAPGGAFVCKVFAGGAEGDILTRLKQNFESVKHVKPKASRSGSAEKYVVATGF 268
[Close]
BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A316GGX9_9RHOB (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Silicimonas algicola TaxID=1826607 RepID=A0A316GGX9_9RHOB)

HSP 1 Score: 57.4 bits (137), Expect = 1.490e-5
Identity = 30/54 (55.56%), Postives = 37/54 (68.52%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGF 1339
            +L  GGTF+ K  AG  E E++   +R FE V  VKPPASRS S EKY++ATGF
Sbjct:  178 VLEEGGTFVAKVLAGGAEGELQLTLKRRFEKVANVKPPASRSDSSEKYVVATGF 231
[Close]
BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A6S6QUQ1_9HYPH (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Terrihabitans soli TaxID=708113 RepID=A0A6S6QUQ1_9HYPH)

HSP 1 Score: 56.6 bits (135), Expect = 2.640e-5
Identity = 30/54 (55.56%), Postives = 36/54 (66.67%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGF 1339
            +LA  G FL K  AG  ERE+    ++ F  VK +KPPASRSGS E Y+LATGF
Sbjct:  175 VLAPNGRFLAKVLAGGTERELLAMLKKDFREVKHIKPPASRSGSSELYVLATGF 228
[Close]
BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A0J9GS43_9RHOB (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Candidatus Rhodobacter lobularis TaxID=1675527 RepID=A0A0J9GS43_9RHOB)

HSP 1 Score: 56.2 bits (134), Expect = 3.730e-5
Identity = 28/54 (51.85%), Postives = 38/54 (70.37%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGF 1339
            +LA GGTF+ K  AG  E ++++  +R F  V  VKPPASRS S EK+++ATGF
Sbjct:  180 VLAEGGTFVAKVLAGGTEGDLQKTLKRRFAKVANVKPPASRSDSSEKFVVATGF 233
[Close]
BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A365U4Q7_9RHOB (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Rhodosalinus halophilus TaxID=2259333 RepID=A0A365U4Q7_9RHOB)

HSP 1 Score: 56.2 bits (134), Expect = 3.910e-5
Identity = 29/54 (53.70%), Postives = 36/54 (66.67%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGF 1339
            +L  GGTF+ K  AG  E  ++ E +R F  V  VKPPASRS S EKY++ATGF
Sbjct:  180 VLEEGGTFVAKVLAGGAEDALQAELKRRFAKVSNVKPPASRSDSSEKYVVATGF 233
[Close]
BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A4Q3Y9A2_9RHOB (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Loktanella sp. IMCC34160 TaxID=2510646 RepID=A0A4Q3Y9A2_9RHOB)

HSP 1 Score: 56.2 bits (134), Expect = 4.040e-5
Identity = 29/54 (53.70%), Postives = 39/54 (72.22%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGF 1339
            +LA GGTF+ K  AG  E E+++  ++ FE V  VKPPASRS S EK+++ATGF
Sbjct:  183 VLAPGGTFVAKVLAGGAEGELQKLLKQRFEKVANVKPPASRSDSSEKFVVATGF 236
[Close]
BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A1I1DM63_9RHOB (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Tropicimonas isoalkanivorans TaxID=441112 RepID=A0A1I1DM63_9RHOB)

HSP 1 Score: 55.8 bits (133), Expect = 5.380e-5
Identity = 29/54 (53.70%), Postives = 38/54 (70.37%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGF 1339
            +LA GGTF+ K  AG  E E+++  +  FE V  VKPPASRS S EK+++ATGF
Sbjct:  178 VLAPGGTFVAKVLAGGAEGELQKLLKNRFEKVANVKPPASRSDSSEKFVVATGF 231
[Close]
BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Match: A0A3M0WK59_9PROT (Ribosomal RNA large subunit methyltransferase E n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A3M0WK59_9PROT)

HSP 1 Score: 55.8 bits (133), Expect = 5.860e-5
Identity = 29/54 (53.70%), Postives = 36/54 (66.67%), Query Frame = 2 
Query: 1178 LLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPASRSGSGEKYLLATGF 1339
            +LA GG F+ K  AG  E E+++  +R F  V  VKPPASRS S EKY+LA GF
Sbjct:  185 VLAPGGVFVAKVLAGGAEGELQKRLKRAFREVHNVKPPASRSDSAEKYVLALGF 238
The following BLAST results are available for this feature:
BLAST of mRNA_P-littoralis_Contig193.63.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 17
Match NameE-valueIdentityDescription
A0A6H5KT20_9PHAE2.350e-7245.07FtsJ domain-containing protein n=2 Tax=Ectocarpus ... [more]
A0A6L5Z4E8_9RHOB1.050e-553.33Ribosomal RNA large subunit methyltransferase E n=... [more]
A0A2S7K0A4_9PROT1.260e-555.56Ribosomal RNA large subunit methyltransferase E n=... [more]
A0A316GGX9_9RHOB1.490e-555.56Ribosomal RNA large subunit methyltransferase E n=... [more]
A0A6S6QUQ1_9HYPH2.640e-555.56Ribosomal RNA large subunit methyltransferase E n=... [more]
A0A0J9GS43_9RHOB3.730e-551.85Ribosomal RNA large subunit methyltransferase E n=... [more]
A0A365U4Q7_9RHOB3.910e-553.70Ribosomal RNA large subunit methyltransferase E n=... [more]
A0A4Q3Y9A2_9RHOB4.040e-553.70Ribosomal RNA large subunit methyltransferase E n=... [more]
A0A1I1DM63_9RHOB5.380e-553.70Ribosomal RNA large subunit methyltransferase E n=... [more]
A0A3M0WK59_9PROT5.860e-553.70Ribosomal RNA large subunit methyltransferase E n=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-littoralis_Contig193contigP-littoralis_Contig193:232631..234821 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Pylaiella littoralis U1_48 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog159749.K0T9C1
PFAMsFtsJ
Model size1810
Max annot lvl2836|Bacillariophyta
Hectar predicted targeting categoryother localisation
Exons2
Evalue5.04e-07
EggNOG OGsCOG0293@1|root,KOG4589@2759|Eukaryota,2XFZK@2836|Bacillariophyta
DescriptionFtsJ-like methyltransferase
Cds size1119
COG categoryD
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789929.6390436-UTR-P-littoralis_Contig193:232630..2330741680789929.6390436-UTR-P-littoralis_Contig193:232630..233074Pylaiella littoralis U1_48UTRP-littoralis_Contig193 232631..233074 -
1680789929.6609297-UTR-P-littoralis_Contig193:234193..2343101680789929.6609297-UTR-P-littoralis_Contig193:234193..234310Pylaiella littoralis U1_48UTRP-littoralis_Contig193 234194..234310 -
1680789929.6768954-UTR-P-littoralis_Contig193:234691..2348211680789929.6768954-UTR-P-littoralis_Contig193:234691..234821Pylaiella littoralis U1_48UTRP-littoralis_Contig193 234692..234821 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789929.6514509-CDS-P-littoralis_Contig193:233074..2341931680789929.6514509-CDS-P-littoralis_Contig193:233074..234193Pylaiella littoralis U1_48CDSP-littoralis_Contig193 233075..234193 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-littoralis_Contig193.63.1prot_P-littoralis_Contig193.63.1Pylaiella littoralis U1_48polypeptideP-littoralis_Contig193 233075..234193 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-littoralis_Contig193.63.1

>prot_P-littoralis_Contig193.63.1 ID=prot_P-littoralis_Contig193.63.1|Name=mRNA_P-littoralis_Contig193.63.1|organism=Pylaiella littoralis U1_48|type=polypeptide|length=373bp
MKSGEETSVVDETTRASLGRNSDGGGDGGVENIGGFNKGKEEEGNVQKDF
NGERVFLPRGTSASRALLPRPEQPSTDPLEELFLAATSTPSVAAEEPTAT
PAAPAAQDIKPSSLVVGGVRRGRVSRDENGANRSPPSAPSSSTPPGSGRR
RRANVVLSDMAPSFSGDRDTDQARVAGLVLDALAACLGDEATRLREQPQR
CGAGAGGSDPQDREARGRSTGAAAYPCRADGDEAMSSTRSSGRILPGKGG
QGSVTDVPGISFPGPGEMNSSSSSRRSDVRGRLTVPCSENERNVGGGGDE
IEEEGSDGTGLLARGGTFLGKFFAGRDEREVKEEAERLFEGVKVVKPPAS
RSGSGEKYLLATGFLLGGRKKR*
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mRNA from alignment at P-littoralis_Contig193:232631..234821-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-littoralis_Contig193.63.1 ID=mRNA_P-littoralis_Contig193.63.1|Name=mRNA_P-littoralis_Contig193.63.1|organism=Pylaiella littoralis U1_48|type=mRNA|length=2191bp|location=Sequence derived from alignment at P-littoralis_Contig193:232631..234821- (Pylaiella littoralis U1_48)
CGCGCCTCCACGGCTGCCCCCGCCGCTCCCCGAAGGACGAGTTGAGACGG
CCGAGAGAAAAGGGCGACGGACAGGGGTGGTGGTGGCGGTCGATCTGCTC
GATCTGGAGGTGGACTTGCCAGGGGTCGAGGTACGTGCGTGCGCAGGAGG
GGGTAGGGGGGTTCCCCCGCCGTAAGGAAAAAGAACGGTTTATCGGCGAC
TGTCGAAAGGTACGGTCTGCCTTTGTTGTGGTGGTCTTCACCACGGCGTT
TCAAATCAAGGGCAGCAGTCGTGGCGGCGGATAAGACACCCCGCCAGACG
TTAGTTGGGGATGGAAAGGTAGAATCCAAGCTCGCGCTCACTGCCGGCAC
CCAGGTCTGTATTTGCGGCGTAACCTCAGCGGCAAAACCGAGCGAATCAG
GATCTGCCCATCCGCACTACCACCACCGCCGCCCCACGCCCTCCACACGC
ATCCACAGCACACACACACATGCACACAAACTCTGCTTCGTGACTGTACG
GACGACTTCAGGCTATTCGAGGAGACTTCCGAGACGTTAGCGTGGAATTG
AAGATACTAGCATCTTTGGCGGCAAGAGAGCGGCGACGTCAGCAGCAGCA
GCAGCAGTGTCCCACAACAGAGGCGACGATGAAGAGTGGGGAAGAAACCA
GCGTCGTCGACGAAACGACGAGGGCGAGTCTTGGTCGAAACAGCGACGGT
GGTGGCGATGGCGGCGTCGAGAACATTGGCGGCTTCAACAAGGGGAAGGA
GGAGGAGGGCAACGTCCAGAAGGACTTCAACGGCGAGCGGGTGTTCCTTC
CCCGGGGTACGTCGGCCTCGAGGGCCCTTCTGCCGAGACCGGAGCAGCCC
TCGACGGATCCCCTGGAAGAGCTTTTCCTCGCCGCAACATCAACACCTTC
AGTAGCGGCGGAGGAGCCGACGGCGACACCAGCAGCACCAGCAGCACAGG
ACATCAAACCGTCGTCGCTTGTTGTGGGCGGTGTTCGTCGGGGCAGGGTC
TCGCGAGACGAGAACGGTGCAAATCGTTCCCCGCCCTCTGCTCCTTCTTC
CTCCACGCCCCCGGGGTCGGGCCGCCGCCGCCGCGCCAACGTCGTCCTGT
CGGACATGGCACCGTCGTTTTCGGGCGACCGAGACACGGACCAAGCGCGT
GTCGCGGGGCTGGTGCTGGATGCCCTCGCGGCGTGTTTGGGTGACGAAGC
CACGAGGCTCAGAGAGCAGCCCCAGAGATGTGGCGCAGGGGCAGGGGGGA
GTGACCCGCAGGACCGAGAGGCACGGGGGCGAAGTACTGGCGCTGCTGCT
TATCCTTGTCGGGCTGATGGGGACGAGGCTATGAGTAGTACTCGCAGCTC
TGGGAGGATCCTGCCGGGGAAAGGAGGGCAGGGCTCAGTGACTGATGTCC
CAGGGATTTCGTTTCCAGGCCCCGGGGAAATGAACAGCAGCAGCAGTAGT
CGTAGAAGCGATGTTCGGGGAAGATTGACGGTTCCGTGTTCCGAAAATGA
GCGAAATGTTGGAGGAGGAGGCGACGAAATAGAAGAAGAGGGGAGCGACG
GAACCGGGCTTCTGGCGCGCGGAGGAACGTTCCTGGGAAAGTTCTTCGCG
GGGAGGGACGAGCGGGAGGTGAAGGAAGAGGCAGAGAGGCTGTTCGAGGG
GGTGAAGGTGGTCAAGCCCCCGGCTTCTCGGTCGGGCTCCGGGGAGAAGT
ACCTTTTGGCCACGGGATTTTTGCTTGGCGGACGCAAGAAGCGCTAAGGA
TGATAGATAGGACATCACAGCATTGGGTACGTATTGCGTGGCATAATTTA
TCTGCCTCGGTGGCACGGAGGCCGGTTTGGACGTCGCGCTTGTACGCCGC
AAGAAGCGGTCATGTTTCCTGTCTTGTCCTTTAGAAATGAACGTGAATTC
GAAGACACATATGTATACCAGGCACCAGGTTACATATTTTAAGGGTTTCC
CCCTCCGCGACATTTGGCACTTCTTGAATTTTATCTAAGGATCATGCGTG
GCGCAACGCAGAAGCTGTGGTACCCATATTCCGTCACGAGTATATAGGCT
AAATACTCTGGGCCACCAAAGAACATGGACGGACGGGCGGAGGCAAAAGA
CAGACAAGGGAGAGGAAATGGTGTGGTCGGTTGTGGTTGTGGTTGTGGTG
GGCGGGGCTCTGATGGTCACCATTTCAAATTTCAAAAACTC
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Coding sequence (CDS) from alignment at P-littoralis_Contig193:232631..234821-

>mRNA_P-littoralis_Contig193.63.1 ID=mRNA_P-littoralis_Contig193.63.1|Name=mRNA_P-littoralis_Contig193.63.1|organism=Pylaiella littoralis U1_48|type=CDS|length=1119bp|location=Sequence derived from alignment at P-littoralis_Contig193:232631..234821- (Pylaiella littoralis U1_48)
ATGAAGAGTGGGGAAGAAACCAGCGTCGTCGACGAAACGACGAGGGCGAG
TCTTGGTCGAAACAGCGACGGTGGTGGCGATGGCGGCGTCGAGAACATTG
GCGGCTTCAACAAGGGGAAGGAGGAGGAGGGCAACGTCCAGAAGGACTTC
AACGGCGAGCGGGTGTTCCTTCCCCGGGGTACGTCGGCCTCGAGGGCCCT
TCTGCCGAGACCGGAGCAGCCCTCGACGGATCCCCTGGAAGAGCTTTTCC
TCGCCGCAACATCAACACCTTCAGTAGCGGCGGAGGAGCCGACGGCGACA
CCAGCAGCACCAGCAGCACAGGACATCAAACCGTCGTCGCTTGTTGTGGG
CGGTGTTCGTCGGGGCAGGGTCTCGCGAGACGAGAACGGTGCAAATCGTT
CCCCGCCCTCTGCTCCTTCTTCCTCCACGCCCCCGGGGTCGGGCCGCCGC
CGCCGCGCCAACGTCGTCCTGTCGGACATGGCACCGTCGTTTTCGGGCGA
CCGAGACACGGACCAAGCGCGTGTCGCGGGGCTGGTGCTGGATGCCCTCG
CGGCGTGTTTGGGTGACGAAGCCACGAGGCTCAGAGAGCAGCCCCAGAGA
TGTGGCGCAGGGGCAGGGGGGAGTGACCCGCAGGACCGAGAGGCACGGGG
GCGAAGTACTGGCGCTGCTGCTTATCCTTGTCGGGCTGATGGGGACGAGG
CTATGAGTAGTACTCGCAGCTCTGGGAGGATCCTGCCGGGGAAAGGAGGG
CAGGGCTCAGTGACTGATGTCCCAGGGATTTCGTTTCCAGGCCCCGGGGA
AATGAACAGCAGCAGCAGTAGTCGTAGAAGCGATGTTCGGGGAAGATTGA
CGGTTCCGTGTTCCGAAAATGAGCGAAATGTTGGAGGAGGAGGCGACGAA
ATAGAAGAAGAGGGGAGCGACGGAACCGGGCTTCTGGCGCGCGGAGGAAC
GTTCCTGGGAAAGTTCTTCGCGGGGAGGGACGAGCGGGAGGTGAAGGAAG
AGGCAGAGAGGCTGTTCGAGGGGGTGAAGGTGGTCAAGCCCCCGGCTTCT
CGGTCGGGCTCCGGGGAGAAGTACCTTTTGGCCACGGGATTTTTGCTTGG
CGGACGCAAGAAGCGCTAA
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