mRNA_P-littoralis_Contig74.85.2 (mRNA) Pylaiella littoralis U1_48

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-littoralis_Contig74.85.2
Unique NamemRNA_P-littoralis_Contig74.85.2
TypemRNA
OrganismPylaiella littoralis U1_48 (Pylaiella littoralis U1_48 (Mung))
Homology
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BLAST of mRNA_P-littoralis_Contig74.85.2 vs. uniprot
Match: D7FQY5_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FQY5_ECTSI)

HSP 1 Score: 628 bits (1619), Expect = 1.030e-216
Identity = 337/530 (63.58%), Postives = 402/530 (75.85%), Query Frame = 2 
Query:  197 LFLCSRSNVACTIRDRGVEVECDLTFSHIPKVLKGCLHTICQNCAEDGLQRSENASSIKCPLCGLTTSDVITTSALQDNIMALQDLHRKNSYCDFCDDDTVQASHVCDECESLLCTFHVKAHAVSRGTKSHTVASLTEKARKGAELGILHCVPITCARHAGVPAKLFCSEPCGATICHNCSVTEHSGHTVYLADSEEVELLHRTGLTRRGVSLGHSLAKVQGALDAVEAVKTGVEQHSRMAEKSAVQVFASIRAAVDSREKAVLENLQADRASKLQDLNKQLNELTETIKAYAAALDTTNESLKYLHGNQLLAMEPTLRECLDKHEANFLSEQLEPCSGDRVEFEYDRDIAIALRLGISQVGYLAQTKADFGSTSDRTHKRGKPRAPEQEVHTSWTSNGHDIHFTLAAIVGARPQEELTLATPTDVVIVEIRNGSELEFLKNGKPGPVIGRVALHTKLKGARHLKYESEHFFESLSQQAAPKWQQRKQLQEGKQQQELSSCHEID-DGIPTVRFTKTLRVSGRRQRVEGS 1783
            +FL  R  +   +R   + +  DLTFSHIPKVLKGCLHTICQNCAE+GLQRS++ SSI CPLCG++TSDV +TSALQDNI+ALQD+HRKNS+CDFCDD   +ASHVC EC SLLCTFH+ AH+VSRGTKSH V SL  +          H V ITCARH  V AKLFCSEPCG TICHNCSV EHSGH+V+ +DS EVE +HR GLTRRGVSLGH LA+V+G LDAVEAVKTGVE+H+R+AE + VQ F++IRAAVDSREK +L+NL+ADRA K QDLN QL +L + ++AY  ALD+TN SL+ LHG+Q LAMEPT+RECLD  EA F S +L PC  DRVEFE+DR IA ALR GIS++G +               K+ K  APEQE+H S T + H+IHF+LAAIVGARPQE  TLA+PTD VIVEIR+GSE +FLK+G  GPVIGRVALHTK KGA+HL YE+EHFFESLSQ+A P  Q RKQ  + +++++       D D +P VRFTK LRV GRR   EGS
Sbjct:    8 IFLLFRRALLTHVRG-DMAIGGDLTFSHIPKVLKGCLHTICQNCAEEGLQRSKDGSSIMCPLCGVSTSDVTSTSALQDNIVALQDVHRKNSHCDFCDD-VAKASHVCHECASLLCTFHMTAHSVSRGTKSHRVVSLKNREAS-------HRVQITCARHRDVEAKLFCSEPCGTTICHNCSVAEHSGHSVFFSDSAEVEQVHRAGLTRRGVSLGHRLARVRGGLDAVEAVKTGVEKHTRLAESATVQAFSAIRAAVDSREKEILKNLRADRAQKTQDLNAQLAKLGQVMRAYEIALDSTNTSLERLHGDQFLAMEPTIRECLDAREAEFPSLELTPCVEDRVEFEHDRSIANALRQGISRLGCVVNAVRP-------RKKKEKSTAPEQELHASSTPSEHNIHFSLAAIVGARPQEA-TLASPTDKVIVEIRSGSEHDFLKSGTTGPVIGRVALHTKFKGAKHLAYETEHFFESLSQRATPTRQSRKQQHQAEKERQKQPTPSRDGDCVPAVRFTKVLRVFGRRG--EGS 518
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BLAST of mRNA_P-littoralis_Contig74.85.2 vs. uniprot
Match: UPI0018855882 (E3 ubiquitin-protein ligase TRIM33-like n=1 Tax=Pollicipes pollicipes TaxID=41117 RepID=UPI0018855882)

HSP 1 Score: 62.8 bits (151), Expect = 1.840e-6
Identity = 49/189 (25.93%), Postives = 82/189 (43.39%), Query Frame = 2 
Query:  257 ECDLTFSH---IPKVLKGCLHTICQNCAEDGLQRSENASSIKCPLCGLTTSDVITTSALQDNIMALQDLHRKNSYCDFCDDDTVQA--SHVCDECESLLCTFHVKAHAVSRGTKSHTVASLTEKARKGAELGILHCVPITCARHAGVPAKLFCSEPCGATICHNCSVTEHSGH---TVYLADSEEVELL 799
             C++   H    PK+L GCLHT+C  CA+       +++  +C LC +T+S  +    L + ++  Q   +++  C  C +   QA  S  C EC + LC   ++AH V++ T  H +    +            C    C+ H       +C + C    C  C ++ H  H   T+   D+E  E L
Sbjct:   77 SCNVLLRHGECTPKLLTGCLHTVCAPCAQQC-----SSAGFQCQLC-MTSSPSLLDHPLVEELLQRQHEEQRSRLCGQCAERGGQAAASGTCTECSTDLCNVCIQAHRVTKPTWGHNIDVCQDS-----------CAQQQCSSHYKTFDN-YC-DTCNTLGCGECILSAHRSHQSCTLLAKDAEVKETL 246
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BLAST of mRNA_P-littoralis_Contig74.85.2 vs. uniprot
Match: A0A0F8B4S9_LARCR (Tripartite motif-containing protein 16 n=1 Tax=Larimichthys crocea TaxID=215358 RepID=A0A0F8B4S9_LARCR)

HSP 1 Score: 59.3 bits (142), Expect = 2.840e-5
Identity = 69/301 (22.92%), Postives = 123/301 (40.86%), Query Frame = 2 
Query:  302 CLHTICQNCAEDGLQRSENASSIKCPLCGLTTSDVITTSALQDNIMA---LQDLHR---------------KNSYCDFCDDDTVQASHVCDECESLLCTFHVKAHAVSRGTKSHTVASLTEKARKGAELGILHCVPITCARHAGVPAKLFC--SEPCGATICHNCSVTEHSGHTVYLADSEEVELLHRTGLTRRGVSLGHSLAKVQGALDAVEAVKTGVEQHSRMAEKSAV---QVFASIRAAVDSREKAVL------ENLQADRASKLQD-LNKQLNELTETIKAYAAALDTTNESLKYL 1114
            C H+ C NC +      +      CP C  T    I    L  N M    ++DL +               ++  CDFC    ++A   C +C+   C  H++ H  S     H +   ++K ++             C+ H  V  K+FC   + C   IC+ CS+ EH GH + +A+ E  E   R   +++ +       ++Q     V+ ++  VE  +  A+K+ +   + F  +   V+ R   V       EN++  R   LQ+ L +++ EL     A    L  T++ +++L
Sbjct:   29 CGHSYCMNCIKSHWDEEDQKEIHSCPQCRQT---FIPRPVLVKNTMLADLVEDLKKTGLQAAPADQCYAGPEDESCDFCTGRKLKAVKSCLQCQVSYCEQHLQPHYESPAFDKHKLVDPSKKLQEN-----------ICSWHNEV-MKIFCRTDQQC---ICYLCSMDEHKGHDIVVAEGERTERQKRLETSQQKIQ-----QRIQDRERDVKLLQQEVEAINHSADKAVMDSEKSFTELVYIVEKRSSDVKQLIRSQENIEVSRVKDLQEKLQQEITELRRK-DAELEQLSNTDDHIQFL 305
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BLAST of mRNA_P-littoralis_Contig74.85.2 vs. uniprot
Match: UPI000F5DED6C (tripartite motif-containing protein 16-like n=1 Tax=Larimichthys crocea TaxID=215358 RepID=UPI000F5DED6C)

HSP 1 Score: 58.5 bits (140), Expect = 3.010e-5
Identity = 69/301 (22.92%), Postives = 123/301 (40.86%), Query Frame = 2 
Query:  302 CLHTICQNCAEDGLQRSENASSIKCPLCGLTTSDVITTSALQDNIMA---LQDLHR---------------KNSYCDFCDDDTVQASHVCDECESLLCTFHVKAHAVSRGTKSHTVASLTEKARKGAELGILHCVPITCARHAGVPAKLFC--SEPCGATICHNCSVTEHSGHTVYLADSEEVELLHRTGLTRRGVSLGHSLAKVQGALDAVEAVKTGVEQHSRMAEKSAV---QVFASIRAAVDSREKAVL------ENLQADRASKLQD-LNKQLNELTETIKAYAAALDTTNESLKYL 1114
            C H+ C NC +      +      CP C  T    I    L  N M    ++DL +               ++  CDFC    ++A   C +C+   C  H++ H  S     H +   ++K ++             C+ H  V  K+FC   + C   IC+ CS+ EH GH + +A+ E  E   R   +++ +       ++Q     V+ ++  VE  +  A+K+ +   + F  +   V+ R   V       EN++  R   LQ+ L +++ EL     A    L  T++ +++L
Sbjct:   29 CGHSYCMNCIKSHWDEEDQKEIHSCPQCRQT---FIPRPVLVKNTMLADLVEDLKKTGLQAAPADQCYAGPEDVSCDFCTGRKLKAVKSCLQCQVSYCEQHLQPHYESPAFDKHKLVDPSKKLQEN-----------ICSWHNEV-MKIFCRTDQQC---ICYLCSMDEHKGHDIVVAEGERTERQKRLETSQQKIQ-----QRIQDRERDVKLLQQEVEAINHSADKAVMDSEKSFTELVYIVEKRSSDVKQLIRSQENIEVSRVKDLQEKLQQEITELRRK-DAELEQLSNTDDHIQFL 305
The following BLAST results are available for this feature:
BLAST of mRNA_P-littoralis_Contig74.85.2 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
D7FQY5_ECTSI1.030e-21663.58Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
UPI00188558821.840e-625.93E3 ubiquitin-protein ligase TRIM33-like n=1 Tax=Po... [more]
A0A0F8B4S9_LARCR2.840e-522.92Tripartite motif-containing protein 16 n=1 Tax=Lar... [more]
UPI000F5DED6C3.010e-522.92tripartite motif-containing protein 16-like n=1 Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-littoralis_Contig74contigP-littoralis_Contig74:378622..381489 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Pylaiella littoralis U1_48 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FQY5
PFAMszf-B_box
Model size2317
Max annot lvl2759|Eukaryota
KEGG koko:K08883
Hectar predicted targeting categoryother localisation
Exons3
Evalue2.85e-182
EggNOG OGsKOG2177@1|root,KOG2177@2759|Eukaryota
Ec32 ortholog descriptionSecreted RING/FYVE/PHD-type and B-box zinc finger protein
Ec32 orthologEc-09_003280.1
EC2.3.2.27
Descriptionzinc ion binding
Cds size1362
COG categoryO
BRITEko00000,ko01000,ko01001,ko04121
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791531.863448-UTR-P-littoralis_Contig74:378621..3790571680791531.863448-UTR-P-littoralis_Contig74:378621..379057Pylaiella littoralis U1_48UTRP-littoralis_Contig74 378622..379057 +
1680791531.9178452-UTR-P-littoralis_Contig74:380970..3814891680791531.9178452-UTR-P-littoralis_Contig74:380970..381489Pylaiella littoralis U1_48UTRP-littoralis_Contig74 380971..381489 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791531.8797107-CDS-P-littoralis_Contig74:379057..3794641680791531.8797107-CDS-P-littoralis_Contig74:379057..379464Pylaiella littoralis U1_48CDSP-littoralis_Contig74 379058..379464 +
1680791531.8909504-CDS-P-littoralis_Contig74:379813..3799611680791531.8909504-CDS-P-littoralis_Contig74:379813..379961Pylaiella littoralis U1_48CDSP-littoralis_Contig74 379814..379961 +
1680791531.9028466-CDS-P-littoralis_Contig74:380163..3809701680791531.9028466-CDS-P-littoralis_Contig74:380163..380970Pylaiella littoralis U1_48CDSP-littoralis_Contig74 380164..380970 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-littoralis_Contig74.85.2prot_P-littoralis_Contig74.85.2Pylaiella littoralis U1_48polypeptideP-littoralis_Contig74 379058..380970 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-littoralis_Contig74.85.2

>prot_P-littoralis_Contig74.85.2 ID=prot_P-littoralis_Contig74.85.2|Name=mRNA_P-littoralis_Contig74.85.2|organism=Pylaiella littoralis U1_48|type=polypeptide|length=454bp
MALQDLHRKNSYCDFCDDDTVQASHVCDECESLLCTFHVKAHAVSRGTKS
HTVASLTEKARKGAELGILHCVPITCARHAGVPAKLFCSEPCGATICHNC
SVTEHSGHTVYLADSEEVELLHRTGLTRRGVSLGHSLAKVQGALDAVEAV
KTGVEQHSRMAEKSAVQVFASIRAAVDSREKAVLENLQADRASKLQDLNK
QLNELTETIKAYAAALDTTNESLKYLHGNQLLAMEPTLRECLDKHEANFL
SEQLEPCSGDRVEFEYDRDIAIALRLGISQVGYLAQTKADFGSTSDRTHK
RGKPRAPEQEVHTSWTSNGHDIHFTLAAIVGARPQEELTLATPTDVVIVE
IRNGSELEFLKNGKPGPVIGRVALHTKLKGARHLKYESEHFFESLSQQAA
PKWQQRKQLQEGKQQQELSSCHEIDDGIPTVRFTKTLRVSGRRQRVEGSQ
SPV*
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mRNA from alignment at P-littoralis_Contig74:378622..381489+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-littoralis_Contig74.85.2 ID=mRNA_P-littoralis_Contig74.85.2|Name=mRNA_P-littoralis_Contig74.85.2|organism=Pylaiella littoralis U1_48|type=mRNA|length=2868bp|location=Sequence derived from alignment at P-littoralis_Contig74:378622..381489+ (Pylaiella littoralis U1_48)
CTCACACACTCTGTATCAGATTAGGCGTCGTCTTCATAAAAACATCAACT
CTAAGTAACGCCGTTTCATGGAACTAATTAACTAGCACTTCGGTTTGACA
TGATCCAAACTACAGCGCAGCAAGTGCGTGCTCAGGATTTTAGTATTTTG
GAAAAATCCAGCGGGTTCTCCTTCAACACAGTTTCTGATTTATGTGCTCT
TTCTCTGTTCCCGCTCCAACGTGGCGTGTACAATCCGTGACAGGGGTGTG
GAAGTGGAGTGTGACCTGACGTTTTCACATATTCCGAAGGTTTTGAAGGG
TTGTTTACACACAATTTGCCAAAATTGCGCAGAGGACGGGCTACAGCGCT
CGGAAAATGCCTCCTCAATCAAGTGTCCATTGTGCGGCTTGACGACTTCC
GACGTCATCACCACCTCTGCCCTGCAAGACAACATCATGGCTCTCCAGGA
TCTTCACCGGAAAAACAGTTATTGCGATTTCTGCGACGACGACACCGTCC
AGGCTTCTCATGTTTGCGATGAGTGCGAGAGCCTCCTCTGCACCTTCCAC
GTGAAGGCCCATGCGGTCTCTCGTGGCACGAAATCGCACACAGTAGCCTC
CCTGACAGAAAAGGCACGGAAGGGCGCTGAACTCGGAATCTTGCATTGTG
TTCCGATCACGTGTGCACGTCATGCTGGTGTTCCCGCCAAGCTGTTCTGC
TCCGAGCCCTGCGGCGCTACCATTTGTCATAATTGCAGTGTGACTGAGCA
CTCTGGTCATACTGTATATCTTGCTGATTCCGAGGAGGTTGAACTGCTTC
ACCGCACCGGCCTTACTCGGCGCGGGGTCTCTTTGGGGCATAGGTACGTA
GTAAGTAGGTGTGGCAGGCTCATGTACAATGGCATTAAAAGTCGTGTTAT
AGTCACCCAAGGGTCTCTCATAGTCTCGATTTGTATTTCGTAGGGTGTTT
TGACCTATCAGGAAACTGCGAGCCACTTTTCCCTTTCTCTCAGGATTCCC
TGACTACAGTATACCCTTCGTACTCGTTCAATGAGAATCTAGCGTTTAGC
AAGGCCGGCTCCAAATGCAAGATTTATCTACGACTTCGAAGTACCAAGCA
TCGTGCAATGAGCACGTAAACCGACCTTAAATACTGTACCTCCGTGGTGC
GACTGGTTTTGCGGTGTCGACGATTCGCCTTTTTCATTACAGTTTAGCTA
AAGTGCAAGGCGCGTTGGACGCGGTCGAGGCGGTCAAAACTGGGGTGGAG
CAACATTCACGAATGGCAGAAAAATCTGCTGTCCAGGTTTTTGCTTCTAT
CCGTGCAGCTGTGGATTCTAGGGAGAAGGCTGTACTCGAGGTATCGGCTT
TTTAAATATGTACTCCGAAGTACTTCAGTTTTGTAGTTGAAACATGCACA
ATTTCGCCAGAGTATTCCAACAAAAGCTTCAACATGTAATGTATCGGACT
AAGAAAGACATGTACATCGTGCAGGCTGTGGCGAATCTCAGCCACCTTTT
CATATGTCTGTGTATTTACTTCGTTCGACCAATCGTTCGCAGAATTTGCA
AGCTGATCGGGCAAGCAAACTACAAGACTTGAACAAGCAATTGAATGAGT
TGACCGAAACGATAAAGGCGTACGCGGCTGCTCTGGACACAACGAACGAA
AGCTTGAAATACTTGCACGGCAACCAGTTGCTGGCGATGGAACCGACTCT
GCGTGAGTGCCTCGACAAGCACGAGGCGAACTTCCTGTCAGAACAGCTCG
AGCCGTGCTCGGGAGACCGTGTGGAGTTTGAGTACGATCGGGATATCGCC
ATCGCTTTGCGACTAGGAATTTCCCAGGTCGGTTACCTCGCACAAACTAA
GGCGGACTTTGGAAGCACTTCGGATCGAACGCACAAACGAGGGAAGCCAA
GGGCTCCAGAACAAGAGGTTCACACATCATGGACATCAAACGGCCATGAC
ATCCACTTCACCCTTGCCGCTATTGTCGGGGCTCGCCCTCAAGAAGAGTT
GACATTGGCAACCCCTACCGATGTAGTTATCGTCGAGATAAGAAACGGGT
CGGAGCTTGAATTTCTGAAAAATGGAAAGCCTGGTCCCGTGATCGGTAGG
GTAGCTCTCCACACGAAGCTCAAAGGTGCCAGGCACCTGAAATATGAGTC
AGAACACTTCTTCGAGTCTTTGTCACAACAGGCTGCCCCCAAATGGCAGC
AGCGGAAGCAGCTTCAGGAAGGAAAGCAGCAACAAGAGTTATCGTCGTGC
CACGAAATCGACGACGGCATTCCGACTGTGAGATTTACCAAGACCTTACG
TGTTTCAGGTCGGCGCCAAAGAGTGGAAGGATCTCAATCACCCGTGTGAG
TATTGCGCACGCGAGAAATCTGACTATCGTTTATGTGTGTTGTCCTTTCG
GTTTATACGGGCGCATCATTAGCCGGAATTACACTTTTTTGTTGCGTTCA
CATTCTATCGGCGGTACTTGCATTACTTTATCGCGGGGAGACTTCCCTTT
CCTTCGTCGACATTCGATCGAAAGTGTCCTTCGTCTTTCGATCGAATGTG
TTGCAACATAAGTAAGTACAAGACAGGTGTTTGCCTTGAGAGCTCCCGAC
AAATTTGGGGGAACTTTGACCCAAAAGCGAAAGAGTGAAAAGATATAAGA
ACATAGACTGAACATCCACGATACAACGAGTGATGTATGTCATGGACTCG
TGAGCGCATGGCATAACCCGCGTATGGTCGATGTGTGATGGTGCACGTCC
GGTAGGTCTTGGAGTTCATTAATGAGTCGATGGGAGACATGGATGCGATT
ACACAAATCGCATTGGAAGAGCAAAATAAAAAATGAATCGAAAGTCGAGT
TGTACATCAATTTGTCCG
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Coding sequence (CDS) from alignment at P-littoralis_Contig74:378622..381489+

>mRNA_P-littoralis_Contig74.85.2 ID=mRNA_P-littoralis_Contig74.85.2|Name=mRNA_P-littoralis_Contig74.85.2|organism=Pylaiella littoralis U1_48|type=CDS|length=1362bp|location=Sequence derived from alignment at P-littoralis_Contig74:378622..381489+ (Pylaiella littoralis U1_48)
ATGGCTCTCCAGGATCTTCACCGGAAAAACAGTTATTGCGATTTCTGCGA
CGACGACACCGTCCAGGCTTCTCATGTTTGCGATGAGTGCGAGAGCCTCC
TCTGCACCTTCCACGTGAAGGCCCATGCGGTCTCTCGTGGCACGAAATCG
CACACAGTAGCCTCCCTGACAGAAAAGGCACGGAAGGGCGCTGAACTCGG
AATCTTGCATTGTGTTCCGATCACGTGTGCACGTCATGCTGGTGTTCCCG
CCAAGCTGTTCTGCTCCGAGCCCTGCGGCGCTACCATTTGTCATAATTGC
AGTGTGACTGAGCACTCTGGTCATACTGTATATCTTGCTGATTCCGAGGA
GGTTGAACTGCTTCACCGCACCGGCCTTACTCGGCGCGGGGTCTCTTTGG
GGCATAGTTTAGCTAAAGTGCAAGGCGCGTTGGACGCGGTCGAGGCGGTC
AAAACTGGGGTGGAGCAACATTCACGAATGGCAGAAAAATCTGCTGTCCA
GGTTTTTGCTTCTATCCGTGCAGCTGTGGATTCTAGGGAGAAGGCTGTAC
TCGAGAATTTGCAAGCTGATCGGGCAAGCAAACTACAAGACTTGAACAAG
CAATTGAATGAGTTGACCGAAACGATAAAGGCGTACGCGGCTGCTCTGGA
CACAACGAACGAAAGCTTGAAATACTTGCACGGCAACCAGTTGCTGGCGA
TGGAACCGACTCTGCGTGAGTGCCTCGACAAGCACGAGGCGAACTTCCTG
TCAGAACAGCTCGAGCCGTGCTCGGGAGACCGTGTGGAGTTTGAGTACGA
TCGGGATATCGCCATCGCTTTGCGACTAGGAATTTCCCAGGTCGGTTACC
TCGCACAAACTAAGGCGGACTTTGGAAGCACTTCGGATCGAACGCACAAA
CGAGGGAAGCCAAGGGCTCCAGAACAAGAGGTTCACACATCATGGACATC
AAACGGCCATGACATCCACTTCACCCTTGCCGCTATTGTCGGGGCTCGCC
CTCAAGAAGAGTTGACATTGGCAACCCCTACCGATGTAGTTATCGTCGAG
ATAAGAAACGGGTCGGAGCTTGAATTTCTGAAAAATGGAAAGCCTGGTCC
CGTGATCGGTAGGGTAGCTCTCCACACGAAGCTCAAAGGTGCCAGGCACC
TGAAATATGAGTCAGAACACTTCTTCGAGTCTTTGTCACAACAGGCTGCC
CCCAAATGGCAGCAGCGGAAGCAGCTTCAGGAAGGAAAGCAGCAACAAGA
GTTATCGTCGTGCCACGAAATCGACGACGGCATTCCGACTGTGAGATTTA
CCAAGACCTTACGTGTTTCAGGTCGGCGCCAAAGAGTGGAAGGATCTCAA
TCACCCGTGTGA
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