mRNA_P-fluviatile_contig12.1509.1 (mRNA) Porterinema fluviatile SAG_2381

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-fluviatile_contig12.1509.1
Unique NamemRNA_P-fluviatile_contig12.1509.1
TypemRNA
OrganismPorterinema fluviatile SAG_2381 (Porterinema fluviatile SAG_2381)
Homology
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BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: A0A6H5KCX6_9PHAE (LHCP protein n=20 Tax=Phaeophyceae TaxID=2870 RepID=A0A6H5KCX6_9PHAE)

HSP 1 Score: 188 bits (477), Expect = 4.510e-53
Identity = 93/103 (90.29%), Postives = 99/103 (96.12%), Query Frame = 2 
Query:    2 AFNGAALTTPAKSSSSMKMAFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEIKHGRISMLAVLGHLTAQNVRLPGMLSTSANLSFADMPNGVAALSKIP 310
            AFNGAA+ TPAKSSS+MKM+FESEIGAQPPLGFWDPLGLLADADQERFERLRYVE+KHGRI+MLA+ GHLT QNVRLPGMLS SANLSFADMPNGVAALSKIP
Sbjct:   22 AFNGAAVATPAKSSSAMKMSFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEVKHGRIAMLAIAGHLTQQNVRLPGMLSNSANLSFADMPNGVAALSKIP 124
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BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: FCPF_MACPY (Fucoxanthin-chlorophyll a-c binding protein F, chloroplastic (Fragment) n=1 Tax=Macrocystis pyrifera TaxID=35122 RepID=FCPF_MACPY)

HSP 1 Score: 185 bits (469), Expect = 5.430e-52
Identity = 92/103 (89.32%), Postives = 98/103 (95.15%), Query Frame = 2 
Query:    2 AFNGAALTTPAKSSSSMKMAFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEIKHGRISMLAVLGHLTAQNVRLPGMLSTSANLSFADMPNGVAALSKIP 310
            AFNGAAL+TPAKSSS+MKM+FESEIGAQ PLGFWDPLGLLADADQERFERLRYVE+KHGRI+MLA+ GHLT QN RLPGMLS SANLSFADMPNGVAALSKIP
Sbjct:   15 AFNGAALSTPAKSSSAMKMSFESEIGAQAPLGFWDPLGLLADADQERFERLRYVEVKHGRIAMLAIAGHLTQQNTRLPGMLSNSANLSFADMPNGVAALSKIP 117
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BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: A0A6H5JHT6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHT6_9PHAE)

HSP 1 Score: 183 bits (464), Expect = 4.000e-51
Identity = 91/103 (88.35%), Postives = 98/103 (95.15%), Query Frame = 2 
Query:    2 AFNGAALTTPAKSSSSMKMAFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEIKHGRISMLAVLGHLTAQNVRLPGMLSTSANLSFADMPNGVAALSKIP 310
            AFNGAA+ T AKSSS+MKM+FESEIGAQPPLGFWDPLGLLADADQERFERLRYVE+KHGRI+MLA+ GHLT QNVRLPGMLS SA+LSFADMPNGVAALSKIP
Sbjct:   25 AFNGAAVATSAKSSSAMKMSFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEVKHGRIAMLAIAGHLTQQNVRLPGMLSNSADLSFADMPNGVAALSKIP 127
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BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: FCPB_MACPY (Fucoxanthin-chlorophyll a-c binding protein B, chloroplastic n=6 Tax=Phaeophyceae TaxID=2870 RepID=FCPB_MACPY)

HSP 1 Score: 180 bits (457), Expect = 3.750e-50
Identity = 90/103 (87.38%), Postives = 96/103 (93.20%), Query Frame = 2 
Query:    2 AFNGAALTTPAKSSSSMKMAFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEIKHGRISMLAVLGHLTAQNVRLPGMLSTSANLSFADMPNGVAALSKIP 310
            AFNGAALTT AK+ S+MKM+FESEIGAQ PLGFWDPLGLLADADQERFERLRYVE+KHGRI+MLA+ GHLT QN RLPGMLS SANLSFADMPNGVAALSKIP
Sbjct:   21 AFNGAALTTSAKACSAMKMSFESEIGAQAPLGFWDPLGLLADADQERFERLRYVEVKHGRIAMLAIAGHLTQQNTRLPGMLSNSANLSFADMPNGVAALSKIP 123
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BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: D8LNK6_ECTSI (Light harvesting complex protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNK6_ECTSI)

HSP 1 Score: 184 bits (468), Expect = 8.040e-50
Identity = 91/103 (88.35%), Postives = 97/103 (94.17%), Query Frame = 2 
Query:    2 AFNGAALTTPAKSSSSMKMAFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEIKHGRISMLAVLGHLTAQNVRLPGMLSTSANLSFADMPNGVAALSKIP 310
            AFNGAA+ TPAKSSS+MKM+FESEIGAQPPLGFWDPLGLL DADQERFERLRYVE+KHGRI+MLA+ GHLT QN RLPGMLS SANLSFADMPNGVAALSKIP
Sbjct:  181 AFNGAAVATPAKSSSAMKMSFESEIGAQPPLGFWDPLGLLEDADQERFERLRYVEVKHGRIAMLAIAGHLTQQNTRLPGMLSNSANLSFADMPNGVAALSKIP 283
[Close]
BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: FCPC_MACPY (Fucoxanthin-chlorophyll a-c binding protein C, chloroplastic n=6 Tax=Phaeophyceae TaxID=2870 RepID=FCPC_MACPY)

HSP 1 Score: 174 bits (440), Expect = 1.110e-47
Identity = 88/103 (85.44%), Postives = 94/103 (91.26%), Query Frame = 2 
Query:    2 AFNGAALTTPAKSSSSMKMAFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEIKHGRISMLAVLGHLTAQNVRLPGMLSTSANLSFADMPNGVAALSKIP 310
            AFNGAALTT AKSSS+MKM+FESEIGAQ PLGFWDPLGLL DADQ+ FERLRYVE+K GRI+MLA+ GHLT QN RLPGMLS SANLSFADMPNGVAALSKIP
Sbjct:   20 AFNGAALTTSAKSSSAMKMSFESEIGAQAPLGFWDPLGLLEDADQDAFERLRYVEVKLGRIAMLAIAGHLTQQNARLPGMLSNSANLSFADMPNGVAALSKIP 122
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BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: FCPD_MACPY (Fucoxanthin-chlorophyll a-c binding protein D, chloroplastic (Fragment) n=2 Tax=Phaeophyceae TaxID=2870 RepID=FCPD_MACPY)

HSP 1 Score: 171 bits (434), Expect = 2.940e-47
Identity = 83/95 (87.37%), Postives = 90/95 (94.74%), Query Frame = 1 
Query:  307 PPLGLAQIVAFVGFLELAVMKNVEGSFPGDFTNGGNPFASSWDAMSEETQESKRAIELNNGRAAQMGILAMMVHEKLSNQPYIINDLVGASYTFN 591
            PP GLAQI  F+GFLELAVMKNVEGSFPGDFT GGNPFASSWDAMSEETQESKRAIELNNGRAAQMGILA+MVHE+L+N+PY+INDL+GASY FN
Sbjct:   88 PPGGLAQIFGFIGFLELAVMKNVEGSFPGDFTLGGNPFASSWDAMSEETQESKRAIELNNGRAAQMGILALMVHEELNNKPYVINDLLGASYNFN 182
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BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: FCPE_MACPY (Fucoxanthin-chlorophyll a-c binding protein E, chloroplastic n=3 Tax=Phaeophyceae TaxID=2870 RepID=FCPE_MACPY)

HSP 1 Score: 172 bits (435), Expect = 5.280e-47
Identity = 84/102 (82.35%), Postives = 93/102 (91.18%), Query Frame = 2 
Query:    5 FNGAALTTPAKSSSSMKMAFESEIGAQPPLGFWDPLGLLADADQERFERLRYVEIKHGRISMLAVLGHLTAQNVRLPGMLSTSANLSFADMPNGVAALSKIP 310
            FNGAAL T AKSSS+MKM+FESEIGAQPP+GFWDPLGL+ADADQERF+RLRYVEIKHGRI+MLAV+GH+T QN RLPGMLS   NL+FAD PNGVAA SKIP
Sbjct:   17 FNGAALATSAKSSSAMKMSFESEIGAQPPIGFWDPLGLVADADQERFDRLRYVEIKHGRIAMLAVVGHITQQNTRLPGMLSFKENLAFADSPNGVAAFSKIP 118
[Close]
BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: A0A6H5JDS8_9PHAE (LHCP protein n=5 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JDS8_9PHAE)

HSP 1 Score: 169 bits (427), Expect = 3.000e-46
Identity = 81/95 (85.26%), Postives = 88/95 (92.63%), Query Frame = 1 
Query:  307 PPLGLAQIVAFVGFLELAVMKNVEGSFPGDFTNGGNPFASSWDAMSEETQESKRAIELNNGRAAQMGILAMMVHEKLSNQPYIINDLVGASYTFN 591
            PP GLAQI AF+GFLELAVMKNVEGSFPGDFT GGNPF SSWDAMSEETQ SKRAIELNNGRAAQMGILA+MVHE+L+N+PY+INDL+GA Y FN
Sbjct:   87 PPAGLAQIFAFIGFLELAVMKNVEGSFPGDFTLGGNPFGSSWDAMSEETQASKRAIELNNGRAAQMGILALMVHEELNNKPYVINDLLGAGYNFN 181
[Close]
BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Match: D8LJH9_ECTSI (Light harvesting complex protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJH9_ECTSI)

HSP 1 Score: 163 bits (413), Expect = 3.140e-45
Identity = 79/95 (83.16%), Postives = 86/95 (90.53%), Query Frame = 1 
Query:  307 PPLGLAQIVAFVGFLELAVMKNVEGSFPGDFTNGGNPFASSWDAMSEETQESKRAIELNNGRAAQMGILAMMVHEKLSNQPYIINDLVGASYTFN 591
            PPLGLAQIVAF+GFLEL VMKNVEGSFPGD T GGNPF + WD MSEET+ SKRAIELNNGRAAQMGILAMM+HE++SNQPYIINDL+ A YTFN
Sbjct:   12 PPLGLAQIVAFIGFLELNVMKNVEGSFPGDMTIGGNPFGAQWDKMSEETKLSKRAIELNNGRAAQMGILAMMIHEEISNQPYIINDLLNAPYTFN 106
The following BLAST results are available for this feature:
BLAST of mRNA_P-fluviatile_contig12.1509.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KCX6_9PHAE4.510e-5390.29LHCP protein n=20 Tax=Phaeophyceae TaxID=2870 RepI... [more]
FCPF_MACPY5.430e-5289.32Fucoxanthin-chlorophyll a-c binding protein F, chl... [more]
A0A6H5JHT6_9PHAE4.000e-5188.35Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
FCPB_MACPY3.750e-5087.38Fucoxanthin-chlorophyll a-c binding protein B, chl... [more]
D8LNK6_ECTSI8.040e-5088.35Light harvesting complex protein n=1 Tax=Ectocarpu... [more]
FCPC_MACPY1.110e-4785.44Fucoxanthin-chlorophyll a-c binding protein C, chl... [more]
FCPD_MACPY2.940e-4787.37Fucoxanthin-chlorophyll a-c binding protein D, chl... [more]
FCPE_MACPY5.280e-4782.35Fucoxanthin-chlorophyll a-c binding protein E, chl... [more]
A0A6H5JDS8_9PHAE3.000e-4685.26LHCP protein n=5 Tax=Ectocarpus sp. CCAP 1310/34 T... [more]
D8LJH9_ECTSI3.140e-4583.16Light harvesting complex protein n=1 Tax=Ectocarpu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-fluviatile_contig12contigP-fluviatile_contig12:2464809..2466394 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Porterinema fluviatile SAG_23812021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score181.8
Seed ortholog evalue3.1e-43
Seed eggNOG ortholog2880.D7G1R7
EggNOG free text desc.Chlorophyll A-B binding protein
EggNOG OGs2CDPH@1,2S3EU@2759
Ec32 ortholog descriptionLight harvesting complex protein
Ec32 orthologEc-07_001470.1
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons2
Model size1548
Cds size594
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-fluviatile_contig12.1509.1prot_P-fluviatile_contig12.1509.1Porterinema fluviatile SAG_2381polypeptideP-fluviatile_contig12 2464809..2465402 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815520.0881107-CDS-P-fluviatile_contig12:2464808..24654021622815520.0881107-CDS-P-fluviatile_contig12:2464808..2465402Porterinema fluviatile SAG_2381CDSP-fluviatile_contig12 2464809..2465402 +
1693562123.6112297-CDS-P-fluviatile_contig12:2464808..24654021693562123.6112297-CDS-P-fluviatile_contig12:2464808..2465402Porterinema fluviatile SAG_2381CDSP-fluviatile_contig12 2464809..2465402 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815520.1238635-UTR-P-fluviatile_contig12:2465402..24654841622815520.1238635-UTR-P-fluviatile_contig12:2465402..2465484Porterinema fluviatile SAG_2381UTRP-fluviatile_contig12 2465403..2465484 +
1693562123.6211426-UTR-P-fluviatile_contig12:2465402..24654841693562123.6211426-UTR-P-fluviatile_contig12:2465402..2465484Porterinema fluviatile SAG_2381UTRP-fluviatile_contig12 2465403..2465484 +
1622815520.147549-UTR-P-fluviatile_contig12:2465524..24663941622815520.147549-UTR-P-fluviatile_contig12:2465524..2466394Porterinema fluviatile SAG_2381UTRP-fluviatile_contig12 2465525..2466394 +
1693562123.6292367-UTR-P-fluviatile_contig12:2465524..24663941693562123.6292367-UTR-P-fluviatile_contig12:2465524..2466394Porterinema fluviatile SAG_2381UTRP-fluviatile_contig12 2465525..2466394 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-fluviatile_contig12.1509.1

>prot_P-fluviatile_contig12.1509.1 ID=prot_P-fluviatile_contig12.1509.1|Name=mRNA_P-fluviatile_contig12.1509.1|organism=Porterinema fluviatile SAG_2381|type=polypeptide|length=198bp
RVQRRRPDHPREVVVFDEDGLRVRDRRAAPPRVLGPARPPCRRGPGAVRA
PPVRGDQARPHLHARRPGPPDRAERPPPRHAVDLRQPVVRRHAQRRGRAL
EDPPLGLAQIVAFVGFLELAVMKNVEGSFPGDFTNGGNPFASSWDAMSEE
TQESKRAIELNNGRAAQMGILAMMVHEKLSNQPYIINDLVGASYTFN*
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mRNA from alignment at P-fluviatile_contig12:2464809..2466394+

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-fluviatile_contig12.1509.1 ID=mRNA_P-fluviatile_contig12.1509.1|Name=mRNA_P-fluviatile_contig12.1509.1|organism=Porterinema fluviatile SAG_2381|type=mRNA|length=1586bp|location=Sequence derived from alignment at P-fluviatile_contig12:2464809..2466394+ (Porterinema fluviatile SAG_2381)
CGCGTTCAACGGCGCCGCCCTGACCACCCCCGCGAAGTCGTCGTCTTCGA
TGAAGATGGCCTTCGAGTCCGAGATCGGCGCGCAGCCCCCCCTCGGGTTC
TGGGACCCGCTCGGCCTCCTTGCCGACGCGGACCAGGAGCGGTTCGAGCG
CCTCCGGTACGTGGAGATCAAGCACGGCCGCATCTCCATGCTCGCCGTCC
TGGGCCACCTGACCGCGCAGAACGTCCGCCTCCCCGGCATGCTGTCGACC
TCCGCCAACCTGTCGTTCGCCGACATGCCCAACGGCGTGGCCGCGCTCTC
GAAGATCCCCCGCTCGGCCTGGCCCAGATCGTTGCGTTCGTGGGCTTCTT
GGAGCTGGCGGTGATGAAGAACGTTGAGGGCTCCTTCCCGGGAGACTTCA
CGAACGGCGGCAACCCGTTCGCGTCGTCTTGGGACGCCATGTCGGAGGAG
ACCCAGGAGTCGAAGCGCGCGATCGAGCTCAACAACGGGCGCGCGGCACA
GATGGGCATCCTGGCCATGATGGTGCACGAGAAGCTCTCCAACCAGCCCT
ACATCATCAACGACCTCGTCGGCGCATCCTACACCTTCAACTGAAAACAG
ATGTGCTTTCGCTGCCTTGAATCATGAGCGAAAACGCGTTGCCCTGAATT
TTTGTGACGATCGACATGAAACTTTAGCCGCGGGGGGCTGTTGTGGGCGT
GTCGTGCATGGCGCAGCCGTGCCTACGCTCCGAGTGCGAGATGTAGACGT
TTCCTTGTTGGGAAGTACAAGTTCCGTCGGCCTCTTTTTTGTTGAAAGGG
CACCTGTAGAATAGGCATTACCCCTTGAACAAAAACAACGAAAATTTTTA
CGAAGCGGCTTTTGAAGCTCTGGTGAGACCGATAGAATAGAAGTGCTTGG
GTGGCAGAAGCAGCAACAACGCGGCGTTTGTGTACTAAGGGAGAAGCTGT
GGACTATCGAATGTCTTTCGCTTCTCCTGTGGGGTCAAGGTGTGTGTCGA
CGCACTTTCTTTTTATTTGCGGTAGAATCGGGAATTTTGTGTGAAGGACG
TGGATGGTGTGGCGCCGTCTGGTCGTTTGACTCCCGAGAGAGGTTGGTTG
AGTTCTTTCGAGCGAGGTCGCGGTCGATTTTGCAGACTTGTCTTCCGTTT
TTTATTCTACGGAGATCCTCGCCCGTTCGCGGGGGGGCCCTTGTTACGAA
AATGCCATGCGGGGTTATATTCCAGTCTCATGTGTCTCATGTGTTCAGTT
CTCGTTGACGTTCGATAGAACGTCTTCGCGGTTTCGTAAACAATCTCGCC
GACTTCCTCAAGTCGAAGCAAAGGGAGCGGGGATGAAGAGAGAGGCGTGC
ACCCACGTTGACCCATCCACCGTCTTCAGTCTGCCGCGCTGGGCGACACA
CTTTTTTGTGTTTATGTTTTGAAGTTCCGGCCAGGAGCCCTGTCGTTACC
CCCCTCGCTCAGCTCTTGTTTCGTTTGCTCTTCGTTCACATTCCCCCAGG
TCTCTTATCTTTTTACTATCACTTAAGTCTCTCTGGTTCATGTAGCAGTT
GCTTGATCAATGTTTCGATTCCCGCTTGTTCAACAC
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Coding sequence (CDS) from alignment at P-fluviatile_contig12:2464809..2466394+

>mRNA_P-fluviatile_contig12.1509.1 ID=mRNA_P-fluviatile_contig12.1509.1|Name=mRNA_P-fluviatile_contig12.1509.1|organism=Porterinema fluviatile SAG_2381|type=CDS|length=1188bp|location=Sequence derived from alignment at P-fluviatile_contig12:2464809..2466394+ (Porterinema fluviatile SAG_2381)
CGCGTTCAACGGCGCCGCCCTGACCACCCCCGCGAAGTCGTCGTCTTCGA
TGAAGATGGCCTTCGAGTCCGAGATCGGCGCGCAGCCCCCCCTCGGGTTC
TGGGACCCGCTCGGCCTCCTTGCCGACGCGGACCAGGAGCGGTTCGAGCG
CCTCCGGTACGTGGAGATCAAGCACGGCCGCATCTCCATGCTCGCCGTCC
TGGGCCACCTGACCGCGCAGAACGTCCGCCTCCCCGGCATGCTGTCGACC
TCCGCCAACCTGTCGTTCGCCGACATGCCCAACGGCGTGGCCGCGCTCTC
GAAGATCCCCCGCTCGGCCTGGCCCAGATCGTTGCGTTCGTGGGCTTCTT
GGAGCTGGCGGTGATGAAGAACGTTGAGGGCTCCTTCCCGGGAGACTTCA
CGAACGGCGGCAACCCGTTCGCGTCGTCTTGGGACGCCATGTCGGAGGAG
ACCCAGGAGTCGAAGCGCGCGATCGAGCTCAACAACGGGCGCGCGGCACA
GATGGGCATCCTGGCCATGATGGTGCACGAGAAGCTCTCCAACCAGCCCT
ACATCATCAACGACCTCGTCGGCGCATCCTACACCTTCAACTGACGCGTT
CAACGGCGCCGCCCTGACCACCCCCGCGAAGTCGTCGTCTTCGATGAAGA
TGGCCTTCGAGTCCGAGATCGGCGCGCAGCCCCCCCTCGGGTTCTGGGAC
CCGCTCGGCCTCCTTGCCGACGCGGACCAGGAGCGGTTCGAGCGCCTCCG
GTACGTGGAGATCAAGCACGGCCGCATCTCCATGCTCGCCGTCCTGGGCC
ACCTGACCGCGCAGAACGTCCGCCTCCCCGGCATGCTGTCGACCTCCGCC
AACCTGTCGTTCGCCGACATGCCCAACGGCGTGGCCGCGCTCTCGAAGAT
CCCCCGCTCGGCCTGGCCCAGATCGTTGCGTTCGTGGGCTTCTTGGAGCT
GGCGGTGATGAAGAACGTTGAGGGCTCCTTCCCGGGAGACTTCACGAACG
GCGGCAACCCGTTCGCGTCGTCTTGGGACGCCATGTCGGAGGAGACCCAG
GAGTCGAAGCGCGCGATCGAGCTCAACAACGGGCGCGCGGCACAGATGGG
CATCCTGGCCATGATGGTGCACGAGAAGCTCTCCAACCAGCCCTACATCA
TCAACGACCTCGTCGGCGCATCCTACACCTTCAACTGA
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