mRNA_P-fluviatile_contig9.15162.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: D7G588_ECTSI (Histidyl-tRNA Synthetase Similar to Histidine Ammonia-Lyase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G588_ECTSI)

HSP 1 Score: 1266 bits (3277), Expect = 0.000e+0
Identity = 710/877 (80.96%), Postives = 761/877 (86.77%), Query Frame = 2

Query:   59 MALEGSAALAVSVGARKVLPQDVLLVAVHGARVDLHPATLDKACPVSCKAPHGGDTPFELSDGAGDTTADSALSEPAARATIFARIVSLMNGRSGLRPEVLIFLCDILNNGITPLLAKEGSDGPSVAKAMLGGGSCMFKGEVRPLVEAATSCGLSPLSMLTAREGKTLVLGRFDRIGLAAMASAAASNAIEIADGVAALACEATRASTAPFDTARFEVRPHRGQIACAERLRLMLEGSKSVNSKKASTAADDDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDCVQPRDGTQTELTVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASKSGPEGLLRELQAVLAEEVALSVALLRXXXXXXXXXXAGPLNVSARPANGGDAPNQASPPGAPGGGGADTTGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRTGEQALGPLLCAMAERLSSGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLPIGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGVRVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASV 2689
            MA EGSAALAVSVGARKVLP+DVLLVAVHGARVDLHPA+LDKACPV      GGD  F +SDGAG+T A+S+ SE AARATIFAR+VSLMNGRSGLRPEVL  LCD+LNNG+TPLL K+G +GPS+A+A+LG GSC+FKGEVRPL+E  +SC LSPLS LT REG TL LGRFD+IG AA+A+AAASNAIEIADGVAAL CEATRA+TAPFD   F+VRPHRGQIACA+RLRLML+GSKS NS K  +  D     PEAISTIPQYHGPARDAIAAA R+IMTEFN SDAGPLGVG+ CVQPRDG QTELT TFL+EAV VL SGCR+R+ AL GS A G  + GG G       G EG+LRELQ+ LAEE+ALSVA+LR XXXXXXXXX                       GAPGGG  DT GMSAAQIAKIEAKRXXXXXXXXXXXXX     KAAGLGVG AEL+Q +APA AAAA +GADEI  +IDPFRTGEQA+GP LCAMAERLS+GG QRKPKIAKGARDFLPEQ +R+RE AFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLPIGNFQIKLNHRRLLDAILDICGCPPEKFR+ICSAIDKLDKMDW +VRQEMVEEKGLAP+SA+KIG FVNGNAG AKELWAKLTEEK FGDHPDA AAMSELELLFKYLDAMGTL+YVSFDLSLARGLTYYTGLIYECVVVDGG+RVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIMEKRLQ+ERG K APSNVQV  +SV
Sbjct:    1 MASEGSAALAVSVGARKVLPEDVLLVAVHGARVDLHPASLDKACPVPSTPSRGGDKAFAVSDGAGETVAESSRSETAARATIFARVVSLMNGRSGLRPEVLTLLCDMLNNGVTPLLPKDGGEGPSLARAVLGAGSCLFKGEVRPLLEVLSSCELSPLSTLTGREGSTLELGRFDQIGTAAVAAAAASNAIEIADGVAALTCEATRANTAPFDALNFDVRPHRGQIACADRLRLMLDGSKSANSTKGDSTVD-----PEAISTIPQYHGPARDAIAAAGRSIMTEFNGSDAGPLGVGDGCVQPRDGKQTELTATFLTEAVRVLTSGCRERSQALQGS-ANGASSCGGGG----DVFGVEGVLRELQSALAEEMALSVAVLRQXXXXXXXXXXXXXXXXXXXXX------XXXXSGAPGGG-VDTAGMSAAQIAKIEAKRXXXXXXXXXXXXXXXXXXKAAGLGVGCAELKQRVAPAGAAAA-VGADEIAAKIDPFRTGEQAMGPFLCAMAERLSNGGAQRKPKIAKGARDFLPEQ-MRVREQAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLPIGNFQIKLNHRRLLDAILDICGCPPEKFRSICSAIDKLDKMDWAEVRQEMVEEKGLAPDSADKIGGFVNGNAGGAKELWAKLTEEKKFGDHPDAVAAMSELELLFKYLDAMGTLQYVSFDLSLARGLTYYTGLIYECVVVDGGIRVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIMEKRLQEERGDKGAPSNVQVRSSSV 858          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: A0A6H5KM67_9PHAE (Histidine--tRNA ligase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KM67_9PHAE)

HSP 1 Score: 1057 bits (2734), Expect = 0.000e+0
Identity = 588/694 (84.73%), Postives = 616/694 (88.76%), Query Frame = 2

Query:  902 MTEFNSSDAGPLGVGEDCVQPRDGTQTELTVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASKSGPEGLLRELQAVLAEEVALSVALLRXXXXXXXXXXAGPLNVSARPANGGDAPNQASPP--GAPGGGGADTTGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRTGEQALGPLLCAMAERLSSGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLPIGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGVRVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASVGPGLLEERMRVGRLLWSNDISAEYSTADNPKFPKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDLPQALLAAGCSVVTAQATL 2977
            MTEFN SDAGPLGVG+ CVQPRDG QTELTVTFLSEAV VL SGCR+R+ AL GS     GA  G GGG+ +  G EG+LRELQ  LAEE+ALSVA+LR  XXXXXXXX                     PP  GAPGGG  DT GMSAAQI KIEAKR  XXXXXXXXXXX     KAAGLGVG AEL+Q +APA AAAA +GADEI  +IDPFRTGEQA+GP LCAMAERLS+GG QRKPKIAKGARDFLPEQ +R+RE AFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILS+LPIGNFQIKLNHRRLLDAILDICGCPPEKFR+ICSAIDKLDKMDW +VRQEMVEEKGLAP+SA+KIG FVNGNAG AKELWAKLTEEK FGDHPDA AAMSELELLFKYLDAMGTL+YVSFDLSLARGLTYYTGLIYECVVVDGG+RVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIMEKRLQ+ERG K APSNVQVY+ASVGPGLLEERMRVGRLLWSNDISAEYST DNPKF KQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRRED+PQALLAAGC++VT QA L
Sbjct:    1 MTEFNGSDAGPLGVGDGCVQPRDGKQTELTVTFLSEAVRVLTSGCRERSQALQGSA---NGASSGGGGGDVT--GVEGVLRELQFALAEEMALSVAVLRQEXXXXXXXXXXXXXXXXX--------XXIQPPQSGAPGGG-VDTAGMSAAQIDKIEAKRAAXXXXXXXXXXXXXXXXKAAGLGVGCAELKQRVAPAGAAAA-VGADEIAAKIDPFRTGEQAMGPFLCAMAERLSNGGAQRKPKIAKGARDFLPEQ-MRVREQAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSALPIGNFQIKLNHRRLLDAILDICGCPPEKFRSICSAIDKLDKMDWAEVRQEMVEEKGLAPDSADKIGGFVNGNAGGAKELWAKLTEEKKFGDHPDAVAAMSELELLFKYLDAMGTLQYVSFDLSLARGLTYYTGLIYECVVVDGGIRVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIMEKRLQEERGDKGAPSNVQVYVASVGPGLLEERMRVGRLLWSNDISAEYSTLDNPKFSKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDVPQALLAAGCTLVTQQAGL 678          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: A0A835Z9D3_9STRA (AA_TRNA_LIGASE_II domain-containing protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9D3_9STRA)

HSP 1 Score: 733 bits (1893), Expect = 1.290e-247
Identity = 475/872 (54.47%), Postives = 567/872 (65.02%), Query Frame = 2

Query:   86 AVSVGARKVLPQDVLLVAVHGARVDLHPATLDK----ACPVSCKAPHGGDTPFELSDGAGDTTADSALSEPAARATIFARIVSLMNGRSGLRPEVLIFLCDILNNGITPLL-AKEGSDGPSVAKAMLGGGSCMFKGEVRPLVEAATSCGLSPLSMLTAREGKTLVLGRFDRIGLAAMASAAASNAIEIADGVAALACEATRASTAPFDTARFEV-RPHRGQIACAERLRLMLEGSKSVNSKKASTAADDDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDCVQPRDGTQTEL-TVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASKSGP-EGLLRELQAVLAEEVALSVA------LLRXXXXXXXXXXAGPLNVSARPANGGDAPNQASPPGAPGGGGADTTGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAG-------LGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRTGEQALGPLLCAMAERLSSGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLPIGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTD------VRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGVRVGSIAAGGRYDNLVGMFSASGQTPCVGVSIGIERVLTIME 2620
            AV+VGARK+L  DV+ VA   A+VDL  ATLD+    A  +  KA  G    F+ +  AG    +S L E  ARA I AR+ SLM  RS +R E +  L  +LN G+ PLL A EG +G +VA+A+LG GS      ++PL  A T+ GL+PL  LT  EG TL L  F   G   MA+AAA  A+++ADG AAL+ +A  A + P   A F+V R HRGQ+  AER+RL+L GS+S+NS  A    DDDA     +  +PQYHGPAR+A+ AA +   TE NS++AGPL    D   P    Q    +   L+ A+ +L+ G R R  A+  +   G+GA         S   P  G L  L+  LAEE+A +           XXXXXXXXXX                   A P            G++  QIAK   K       XXXXXXX                LG GTAE+++ +  AA       A+ +   +D    GE  LG ++  + E+L SGG +RKPK+ KG RD LPEQ +R+RE AF AIR VFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLAL+ VGNIKRFHIAKVYRRDQPN+  GR+REFYQCDFDIAG YG MV DAEVLS ACEILS LPIG+FQ+KLNHRRLLDAILDICGCP EKFRTICSAIDKLDK  W +      VR+EMVEEKGLAPE+A+KIG FV   +G  ++LW +LT+E  FG HPDAAAAM EL LLF YLDAM +L+++ FDLSLARGLTYYTGLIYECVV+DG  +VGSIAAGGRYDNLVGMFS++ QTPCVGVSIGIERV  IME
Sbjct:    1 AVTVGARKLLNADVMAVATGAAKVDLDAATLDRCGAEAQYLKSKASPGATAAFDAAALAG-IPEESVLPEAEARAAIVARLSSLMQCRSCIRAEAVSLLASLLNAGLCPLLPASEGDEGAAVARALLGMGSAYTPTGIQPLAGAMTTAGLAPLQELTTMEGDTLRLRAFAATGATCMAAAAAXTALDLADGAAALSLDACAAFSEPLQGAHFDVYRQHRGQMEVAERVRLLLTGSRSLNSPVAPVYQDDDA-----LRAVPQYHGPAREAVLAAGKVAETELNSAEAGPLTRRHDSAGPYHPQQASCCSYQPLAHALLILMQGSRARLAAMSAAAGNGIGA---------STYVPLRGALSSLRRALAEELAAAATRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADP----------DAGLTPEQIAKRREKAEKNAALXXXXXXXXXXXDXXXXXXXXXXLLGDGTAEIKRRVMAAAGGEGEPSAEAVLSAVDFNCAGEGTLGGVIAGLIEKLGSGGARRKPKLPKGTRDQLPEQ-MRVREQAFEAIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALNSVGNIKRFHIAKVYRRDQPNLARGRYREFYQCDFDIAGAYGPMVADAEVLSAACEILSGLPIGDFQVKLNHRRLLDAILDICGCPAEKFRTICSAIDKLDKAPWEEASDHHRVRKEMVEEKGLAPEAADKIGGFVT-YSGPPRQLWQQLTDEAKFGQHPDAAAAMGELALLFDYLDAMDSLRFIQFDLSLARGLTYYTGLIYECVVLDGETKVGSIAAGGRYDNLVGMFSST-QTPCVGVSIGIERVFAIME 844          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: A0A2D4BVR0_PYTIN (Histidine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BVR0_PYTIN)

HSP 1 Score: 602 bits (1551), Expect = 1.780e-195
Identity = 429/970 (44.23%), Postives = 544/970 (56.08%), Query Frame = 2

Query:   89 VSVGARKVLPQDVLLVAVHGARVDLHPATLDKACPVSCKAPHGGDTPFELSDGAGDTTADSALSEPAARATIFARIVSLMNGRSGLRPEVLIFLCDILNNGITPLLAKEGSDGPSV----AKAMLGGGSCMFKGEVRPLVEAATSCGLSP-LSMLTAREGKTLVLGRFDRIGLAAMASAAASNAIEIADGVAALACEATRASTAPFDTARFEV-RPHRGQIACAERLRLMLEGSKSVNSKKASTAADDDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDCVQPRDGTQTELTVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASKSGPEG-----LLRELQAVLAEEVALSVALLRXXXXXXXXXXAGPLNVSARPANGGDAPNQASPPGAPGGGGADT--TGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRTGEQALGPLLCAMAERLSSGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLP-IGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGVRVGSIAAGGRYDNLVGMFSASGQT-PCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASVGPGLLEERMRVGRLLWSNDISAEYSTADNPKFPKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDLPQALLAAGCS 2953
            V++   K+  +DV  VA  GA+V    A    A  V   AP  GD    +   +    A  AL   AA A +  R+V L    S     +  F+ D+LN+ + PLL    +D   +    A    G       G+++ L  A     ++P +  L+ RE   L       + +  +  + A   + +AD VAAL CE  +AS+ PF+    +  RPHRG +  A  LRLMLE SK VN+ K       DA   + I  IPQYHGPARDA+AAA + +  E NS  A P   G     P+   +T L  T   +A+ VL+ G  +R   +                 EAS   P+      L+      L  E  L+ A                  + A+ A       +A+          D     M  AQ  KI  KR  XXXXXXXXXXX      +  +G G+A LRQ +    A AA + A         F          +  +  RL SGG +RKPKIAKGA+DFLP Q +++RE  F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++ALH  GNIKR+HIA+VYRRD P +  GRFREFYQCDFDIAG Y  M+PDAEVLS+  E+L   P +G F++KL+HR LLDAIL ICG P +KFRT CSAIDKLDK  W  VR EMV+EKG+    A++I  FV    G  +EL   +TEEK FGD+ DAA AM EL  LF+YLDAMG L +VSFDLSLARGL YYTGLIYE V+      VGSIAAGGRYDNLVGMFSA+GQ  PCVGVS+GIER+  I++   +  +      +  QV +AS G  LL ER+ V + LW  +ISAE    +NPKF KQ+   L+ GIPFMV++GE E+ +G V++KDM    +  + R ++   LL   C+
Sbjct:    5 VTIAGAKLQLEDVCSVAALGAKVAADVA----AQSVDHDAPMMGDEVVAVELPSDAAAAHGALVTRAALAVLLDRVVRLP--ASAATEGLARFIADLLNHNVLPLLPSGSTDATVLVVLAAAQSRGLARVGTDGKIQELSMALMDASIAPPVWALSRRELAALSETPVVLLAVTTLVVSGARGLLPVADAVAALTCEVVQASSLPFEAEYVDASRPHRGIVTSATNLRLMLETSKFVNAPK-------DAPDADCIRHIPQYHGPARDAVAAAAKTVELELNS--AVPTTAG--WFHPQ-ALKTVLASTV--DALTVLLQGSNERRRVVAP---------------EASAIAPQSQCAVELVEVTTQALVAEAELAFAFFDKEEQ----------QLKAKQAESNARAQEAAEKXXXXXXXEDEKLAAMPEAQRLKILEKRRKXXXXXXXXXXXXXXXXDSVRVGAGSAALRQHVQSLGAWAAHVNA---------FDARPSGFSQFVEELFARLGSGGARRKPKIAKGAQDFLPPQ-MQLREKIFNKIRMVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMALHNPGNIKRYHIARVYRRDNPQMARGRFREFYQCDFDIAGTYAPMLPDAEVLSIGIEVLQQFPELGPFKVKLSHRVLLDAILAICGVPADKFRTTCSAIDKLDKEPWAAVRSEMVDEKGIPEAVADRIQHFVT-RVGSPRELHRAMTEEKLFGDNADAARAMGELGTLFEYLDAMGVLDFVSFDLSLARGLDYYTGLIYEFVLTSPEHNVGSIAAGGRYDNLVGMFSATGQQIPCVGVSMGIERIFGILQAHAEKTQAINRPTA--QVLVASTGSSLLLERLSVCKELWRANISAEIMQIENPKFKKQLQHALETGIPFMVVIGEDELAKGEVKVKDMASKEEVSVARSEMVAELLRRSCA 916          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: A0A7S2LH49_9STRA (Histidine--tRNA ligase n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2LH49_9STRA)

HSP 1 Score: 602 bits (1551), Expect = 2.630e-195
Identity = 378/784 (48.21%), Postives = 477/784 (60.84%), Query Frame = 2

Query:  632 IADGVAALACE--ATRASTAPFDTARF-EVRPHRGQIACAERLRLMLEGSKSV--NSKKASTAADDDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDCVQPRDGTQTELTVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASKSGPEGLLRELQAVLAEEVALSVALLRXXXXXXXXXXAGPLNVSARPANGGDAPNQASPPGAPGGGGADT-TGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRT-----GEQALGPLLCAMAERLSSGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLPIGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGVRVGSIAAGGRYDNLVGMFSASGQ-TPCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASVGPGLLEERMRVGRLLWSNDISAEYSTADNPKFPKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDLPQALLAAG 2947
            I+D +A+L+ E   +  S   F +  + E+RPHRG I  A  +R  L+GS +V  N K  + ++DD     +     PQYHGPAR++I  AC+ +  E N S+       +D V          +V  LS    +      D   AL  S    +         E + S  E  +  LQ  L  E  +    +                  A+        N    P A GG G D   GMS AQ AKI                 KR +K+                 AAA  +  GA      I P  +     G++AL   L    E L SGG QRKPK+AKG RD+LPEQ + IR+ AFT IR VFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR+LA++ VGNIKRFHI KVYRRDQP +  GR+REFYQCDFDIAG YG MVPD+E L+VACEIL SLPIG+F IKLNHRRLLDAILD+CG P +KFRTICSA+DKLDK  W++VR+EMVEEKGL+ + A+KIG FV    G   +L+  L E K+FG+H  A+ AM +L +LF+YL+AMG LK++SFDLSLARGL YYTG+IYE V ++G  +VGSI  GGRYD LV MF  +G+ TPCVGVS+GIERV T+ME+RL+ E+G      NV V +AS G  +L ERM++  +LW  +ISAE+S  +NPK   ++   L R IPFMVI+GE+E +QG  ++KD+   T++ +   DL + L + G
Sbjct:  171 ISDPIASLSIERLGSALSIDSFSSTNYDELRPHRGCIESASVIRACLQGSTNVAANEKNVTPSSDDSC---KYAKLTPQYHGPARESITGACKTMELEMNCSEMNGSASLDDTVAMLAAKSVLESVLTLSTGSSMRCGSKTDTTQAL--SAMCNLA--------EVAPS-LETAVVTLQNSLESEAKMGCVFVDDELAKKEAELKAKEEEKAKRTAARGTNNN---PNASGGDGKDEFAGMSEAQKAKI---------------LKKRAEKEXXXXXXXXXXXXXXAGGAAALTSIFGAG--IAAIYPLLSTKSDLGDEALVSELEQAIEALLSGGMQRKPKVAKGTRDYLPEQ-MAIRDKAFTIIRRVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFLAVNAVGNIKRFHIGKVYRRDQPQLSKGRYREFYQCDFDIAGVYGRMVPDSECLAVACEILDSLPIGDFGIKLNHRRLLDAILDLCGVPADKFRTICSAVDKLDKEPWSEVRREMVEEKGLSGDVADKIGEFVV-LKGKPWDLYNSLMESKSFGNHKGASEAMEDLRILFEYLEAMGKLKFISFDLSLARGLDYYTGVIYEAVCMNGNTQVGSIGGGGRYDTLVSMFQEAGKVTPCVGVSVGIERVFTLMEERLRQEQGGSIKQPNVTVLVASAGDNMLRERMKLTNVLWDANISAEFSQQENPKLKFEIANALDRQIPFMVIVGEEETKQGKCKVKDLNARTEDTVDVSDLVKTLRSKG 918          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: A0A8K1C9F9_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1C9F9_PYTOL)

HSP 1 Score: 600 bits (1547), Expect = 9.440e-195
Identity = 408/875 (46.63%), Postives = 508/875 (58.06%), Query Frame = 2

Query:  353 EVLIFLCDILNNGITPLLAKEGSDGPSVA--KAMLGGGSCMFKGEVRPLVEAATSCGLSPLSMLTAREGKTLVLGRFDRIGLAAMASAAASNAIEIADGVAALACEATRASTAPFDTARFEV-RPHRGQIACAERLRLMLEGSKSVNSKKASTAADDDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDC--VQPRDGTQTELTVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASKSGPEGLLRELQAV--LAEEVALSVALLRXXXXXXXXXXAGPLNVSARPANGGDAPNQASPPGAPGGGGADTTGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRTGEQALGPLLCAMAERLSSGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLP-IGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGVRVGSIAAGGRYDNLVGMFSASGQT-PCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASVGPGLLEERMRVGRLLWSNDISAEYSTADNPKFPKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDLPQALLAAGC 2950
            E + FL  +LN  + PLLA    D  ++A   A  G G          L     S G++  S L+A E    V      + +A +    A   + +AD VAAL CE  +AS+ PF+    +V RPHRG +  A  LRLMLE SK +NS+K +      AA  E+I  IPQYHGPARDA+ AA +A+  E NS+      V +      P+   QT L  +   EA+ VL+ G   R                     +A+ S  E L         L E          XXXXXXXXXX                                   M  AQ  KI  KR        XXXX K+  K+   +GVG+A LRQ +    A  AT        R++ F     A    L  +  RL SGG +RKPKIAKGA+DFLP Q +++RE  F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++A+H  GNIKR+HIA+VYRRD P +  GRFREFYQCDFDIAG+Y  M+PDAEV+S+  E+L   P +G F++KL+HR LLDAIL ICG P EKFRT CSAIDKLDK  W  VR EMV+EKGL  E A++I  FV  + G  +EL AKLT E  FGD+ +A  AM EL LLF YLDAMG L ++SFDLSLARGL YYTGLIYE V+      VGSIAAGGRYDNLVGMFSA+GQ  PCVGVS+GIER+  I++   +       A S  QV +AS G  LL  R+ + + LW  +I+AE    +NPKF KQ++  L+ GIP+MV++GE E+ +G V++K+M+   +  + R D+   LL   C
Sbjct:   92 ETVQFLVALLNEDVLPLLASGPEDTAALAVLAAAEGRGFARVNDAAVELQSVLQSAGIALPSPLSATEKLAFVQIPAVLLAVATLTVGGARGLLPVADAVAALTCELLQASSVPFEAEFVDVSRPHRGIVTSATNLRLMLENSKYINSQKVA------AADAESIRYIPQYHGPARDAVLAAFKAVELELNSAVTTDSAVIKSAGTFHPQ-ALQTTLASSI--EALNVLLKGSNSRR--------------------QAADSSAEALAATSSCAIELVEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDEKLAAMPEAQRLKILEKRRIKLEKQKXXXXAKKGGKEEIKIGVGSAALRQYVLTQGAEWAT--------RVNAFDLRVTAFPMFLEDLFLRLGSGGSRRKPKIAKGAQDFLPPQ-MQLREKIFNKIRMVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMAMHNPGNIKRYHIARVYRRDNPQMARGRFREFYQCDFDIAGSYAPMLPDAEVISIGIEVLQQFPELGPFKVKLSHRLLLDAILAICGVPAEKFRTTCSAIDKLDKEPWASVRSEMVDEKGLPEEVADRIEHFVC-HVGSPRELHAKLTNENLFGDNAEAKRAMEELRLLFDYLDAMGVLPFISFDLSLARGLDYYTGLIYEFVLTSPEHNVGSIAAGGRYDNLVGMFSATGQQIPCVGVSMGIERIFGILQAHAEKTSSMNKATS--QVLVASTGGALLLPRLELCKELWRANIAAEIMQLENPKFTKQLHHALESGIPYMVVIGEDELAKGEVKIKEMSSKDEVTVARSDMVAELLRRNC 925          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: A0A1V9Z5Y5_9STRA (Histidine--tRNA ligase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9Z5Y5_9STRA)

HSP 1 Score: 600 bits (1547), Expect = 9.720e-195
Identity = 417/971 (42.95%), Postives = 545/971 (56.13%), Query Frame = 2

Query:   89 VSVGARKVLPQDVLLVAVHGARVDLHP-ATLDKACPVSCKAPHGGDTPFELSDGAGDTTADSALSEPAARATIFARIVSLMNG---RSGLRPEVLIFLCDILNNGITPLLAKEGSDGPSVAKAML----GGGSCMFKGEVRPLVEAATSCGLSPLSMLTAREGKTLVLGRFDRIGLAAMASAAASNAIEIADGVAALACEATRASTAPFDTARFE-VRPHRGQIACAERLRLMLEGSKSVNSKKASTAADDDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDCVQPRDGTQTELTVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASKSGPEGLLRELQAVLAEEVALSVALLRXXXXXXXXXXA---GPLNVSARPANGGDAPNQASPPGAPGGGGADTTGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRTGEQALGPLLCAMAERLS--SGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLP-IGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGV-RVGSIAAGGRYDNLVGMFSASG-QTPCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASVGPGLLEERMRVGRLLWSNDISAEYSTADNPKFPKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDLPQALLAAGC 2950
            V++  +K+  +++  +A  GA+V + P A ++    ++ KA      P          TA ++L +  ARA + ARI  ++     RS     +  FL ++LN+ + P+L    S+    A  +L     G   +F  + + ++  A      P + L   E  T   G      + A   A A   + IAD VAAL CE  +A TAPF+    +  RPHRG I  A  LR ML+GSK+ N       A  D+  P+AI  IPQYHGPARD IA A +AI  E N+ + G      D +        + +++ +  A+  L  G  DR   L  +  A V  +      E S+     LL+    VL  E+ +S+  L               GP   +A      +A  +           A    M  AQ  KI  KR             K  K +    G GT ELR       A  A +G+  +     PF     +L  LL  +  RL   SGG++R+PKIAKGA+DF P Q +++RE  F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARY+ALH  GNIKR+ IA+VYRRD P +  GRFREFYQCDFDIAG Y LM+ DAEV+S+  E+L   P +G+F+IKL+HR+LLDAILD+CG P +K RT CSAIDKLDK  W  VR EMV+EK +APE A++I +FV    G  +EL AKL  EK FG +  AA AM EL LLF YLDAMG L YVSFDLSLARGL YYTGLIYE V+      +VGSIAAGGRYDNLVGMFSA+  Q PCVGVS+GIER+  +++K  +D +    A +  QV +A     LL  R+ V + LW N+I+AE   ++NPKF KQ+   L  GIP++V++GE E+  G V +K +    +  + R +L   L A GC
Sbjct:    5 VTLSTKKLSLEELSSIAFCGAKVAIEPFAPIELPAAIAAKA-----VPLPT-----PVTAATSLPKGIARALLVARINYILKHSELRSSAALAIAEFLVNLLNDDLVPVLPLSSSEAVDAAALVLLDVCAGRGSVFGPDGKAIMAIADVVKTFPAA-LAPIEVATFAQGNATLEAIVAATVAGAKGLLPIADAVAALTCEVRQAQTAPFNVEFHDSARPHRGIITSATHLRAMLDGSKATN-------AHTDSQDPDAIRCIPQYHGPARDTIAQAAKAIEVELNAVENGLYSTTRDGIGAFHPELLKTSLSMVQVALQSLAQGATDRLSLLMHTALAPVVFN------ERSQLSTFELLQATIGVLEHELQVSLQGLADADAALAKQAEKAKGPTMSAAAMQALAEAEAKED---------AKLAAMPEAQRNKILEKRRLKAEKAKEKQSKKAAKDELR-YGAGTTELR-------AHFAAVGSTALV----PFDFRPASLHSLLEELFARLGAKSGGQRREPKIAKGAQDFKPHQ-MQLREEIFHKIRAVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYMALHSPGNIKRYAIARVYRRDNPQMARGRFREFYQCDFDIAGTYPLMLADAEVISIGIEVLKEFPELGSFKIKLSHRKLLDAILDLCGVPADKIRTTCSAIDKLDKETWETVRAEMVDEKDVAPEVADRIQAFVT-QVGSPRELHAKLLAEKRFGTNAPAAEAMKELALLFNYLDAMGVLDYVSFDLSLARGLDYYTGLIYEFVLTGSNAGQVGSIAAGGRYDNLVGMFSATNAQIPCVGVSMGIERIFGMLQKHAEDTKTAAKAAA--QVLVAQTSKDLLIARLAVCKELWQNNIAAEILPSENPKFVKQLQYALDAGIPYVVVIGEDEIAAGNVNVKVLATKEEFTVPRSELVATLAAHGC 926          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: K0RV05_THAOC (Histidine--tRNA ligase (Fragment) n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RV05_THAOC)

HSP 1 Score: 595 bits (1535), Expect = 1.910e-194
Identity = 377/810 (46.54%), Postives = 484/810 (59.75%), Query Frame = 2

Query:  599 MASAAASNAIEIADGVAALACE--ATRASTAPFDTARF-EVRPHRGQIACAERLRLMLEGSKSVNSKKASTAAD-DDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDCVQPRDGTQTELTVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASK-----SGPEGLLRELQAVLAEEVALS----VALLRXXXXXXXXXXAGPLNVSARPANGGDAPNQASPPGAPGGGGADTTGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRTGEQ------ALGPLLCAMAERLSSGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLPIGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGVRVGSIAAGGRYDNLVGMFSASGQ-TPCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASVGPGLLEERMRVGRLLWSNDISAEYSTADNPKFPKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDLPQALLAAGCSVVTAQ 2968
            +A A + +A  + D ++AL+ E      +   F T  + E+RPHRG +  A  LR  L GS  V S K     +  DA   +   + PQYHGPAR+AIA+AC+ +  E N S+ G     +D +         L        +  L++G   R           +G+      G  S      +  E  + +L+A L  E  L     V+ L           A     +A  A  G+ PNQ        G   +  GM+ AQ AKI                 KR +K+A                 AA ++  G    F+   P  +G+       AL   L    E+L SGG QRKPK+A G RD+LPEQ + IR+ AF+ IR VF RHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGE+LALRYDLTVPFAR+LA++ VGNIKRFHI KVYRRD+P +  GR+REFYQCDFDIAG+YG MVPD+E L+VACEIL +LPIG+F IKLNHRRLLDAILDICG P +KFRTICSA+DKLDK  W++VR+EMVE+KGL  + A++IG FV    G+   ++  L E   FGDH  AA AM +L + F YL+AM  LK++SFDLSLARGL YYTG+IYE V ++G  +VGSI  GGRYDNLV MF  +G+ TPCVGVS+GIERV T+ME+RL+ E+G      NV+V +AS G  LL ERMR+ R+LW  +ISAE+S  +NPK   ++   L R IPFMVI GE+E ++G  ++KD+   T+E +   DL   L   G + +  Q
Sbjct:   57 LAQARSLSAKSVCDPISALSAERLGQYLTLDAFSTTNYDELRPHRGCVESASVLRTCLAGSTMVASSKTLKQPEGSDATCVKYAKSAPQYHGPAREAIASACKTMELELNCSEGGDAAELDDSI-------ALLACASALGGITTLVNGSSSR-----------IGSDSKCDAGSPSTLSTMANALESAVEQLKAALESEAKLGCDYVVSELSKREAELKAKEAEKAKRTAERAGSGN-PNQK-------GDKDEFAGMTEAQKAKI---------------LKKRAEKEAXXXXXXXXXXXXAAGGEAALSSIFGTGTAFLY--PLLSGKDLDLSNSALLESLEGAIEKLQSGGMQRKPKVALGTRDYLPEQ-MAIRQQAFSIIRRVFLRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGEMLALRYDLTVPFARFLAVNSVGNIKRFHIGKVYRRDRPVMSKGRYREFYQCDFDIAGHYGRMVPDSECLAVACEILDALPIGDFGIKLNHRRLLDAILDICGVPADKFRTICSAVDKLDKEPWSEVRREMVEDKGLTVDVADRIGEFVV-LKGEPWAMYNSLIEGGKFGDHKGAADAMEDLRICFTYLEAMDKLKFISFDLSLARGLDYYTGVIYEAVCMNGNTQVGSIGGGGRYDNLVSMFQEAGKVTPCVGVSVGIERVFTLMEERLRKEQGGSIKRPNVKVLVASAGENLLHERMRLARVLWDANISAEFSQQENPKLKFEIANALDRQIPFMVISGEEEAREGKCKVKDLANRTEETVAVSDLVTILCGKGVAPLILQ 821          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: A0A662YHR8_9STRA (Histidine--tRNA ligase n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662YHR8_9STRA)

HSP 1 Score: 598 bits (1541), Expect = 6.900e-194
Identity = 425/971 (43.77%), Postives = 554/971 (57.05%), Query Frame = 2

Query:   77 AALAVSVGARKVLPQDVLLVAVHGARVDLHPATLDKACPVSCKAPHGGDTPFELSDGAGDTTADSALSEPAARATIFARIVSLMNGRSGLRPEV-LIFLCDILNNG-ITPLLAKEGSDGPS-VAKAMLGGGSCMFKGEVRPLVEAATSCGLSPLSMLTAREGKTLVLGRFDRIGLAAMASAAASNAIEIADGVAALACEATRASTAPFDTARFEV-RPHRGQIACAERLRLMLEGSKSVNSKKASTAADDDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDCVQPRDGT-----QTELTVTFLSEAVGVLISGCRDRALALGGSGAAGVGAHGGEGGGEASKSGPE-GLLRELQAVLAEEVALSVALLRXXXXXXXXXXAGPLNVSARPANGGDAPNQASPPGAPGGGGADTTGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGAD-EIFVRIDPFRTGEQALGPLLCAMAERLSSGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLP-IGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVVDGGVRVGSIAAGGRYDNLVGMFSASGQT-PCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASVGPGLLEERMRVGRLLWSNDISAEYSTADNPKFPKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDLPQALLAAGC 2950
            AA+ V++GA K+   DV  VA  GA+V +  A        S  AP        LSD   D  +  AL  P  RA +   +  L+  R+    +    F+  +LN+  +TPL+    +D    V  A   G   +  G +  +++ A+     PLS   A E   +       +  A +A   A   + +AD VAA++CE  RAS+A F+    +  RPHRG +  A+ LRLML+GSK VNS+   + A  D A   A+S IPQYHGPARDA+  A + +  E N +      VG+     R G        ++ +   ++A+GVL+ G  DR   L    A            +A K+     L+    A L +E+  S+A             A   N  A+ A                        M  AQ  KI  KR    XXXXXXXXX    K    +G+G+  LRQ L         +GA  +  V+   F      L   L  +  RL SGG +RKPKIAKG++DFLP Q + +RE  F  IR VFKRH  VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++ALH  GNIKR+HIA+VYRRD P +  GRFREFYQCDFDIAG+Y  M+PDAEVLS+  E+L   P +G  ++KL+HR LLDAIL ICG P +KFRT CSAIDKLDK  W++VR+EMVEEK +    A++I  FV    G  +EL A+L +E  FGD+ +A  AM++L LLF YLDAMG L ++SFDLSLARGL YYTGLIYE V+      VGSIAAGGRYDNLVGMFSA+ Q  PCVGVS+GIER+  I++     +  +   PS  QV +AS G  LL  R+ + + LW+ +ISAE    +NPKF KQ++  L+RG P+MV++GE E+ + VV++KDM    +  + R +L   LL  GC
Sbjct:    2 AAVRVTLGAGKLSIADVCCVAALGAQVAVE-AGSPHGGSASAAAPAAPTADVTLSD---DEASSYAL--PTTRAMLTLLLHRLLRLRASAATQANAAFVATLLNHAELTPLVPMSAADSAVLVLLAATEGRGFVRSGPMGQVLQRASVALPDPLS---AAEKTAVTSVPLALLAEATLAVGGARALLPVADAVAAVSCETLRASSAAFEADFVDAARPHRGIVTSAQNLRLMLDGSKFVNSQTEGSNATTDMA---AVSCIPQYHGPARDAVLVAYKTVEAEINGA------VGDSAAAKRAGAGVHPQAVKMALAATTQALGVLLQGSVDRLQVLDSKAA------------DAPKTTDAVELVEATAAALVQELVPSLAFFDEEEAQLKLRVA-EKNAKAQEAXXXXXXXXXXED-------EKLAAMPEAQRNKILEKRRKKLXXXXXXXXXXXXAKDELKIGLGSLALRQHLVG-------LGASWQASVQGAAFDLRVSGLSLFLEELFVRLGSGGARRKPKIAKGSQDFLPHQ-MALREKIFNKIRMVFKRHAGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMALHSPGNIKRYHIARVYRRDNPQMARGRFREFYQCDFDIAGSYAPMLPDAEVLSIGIEVLQQFPELGPVKVKLSHRLLLDAILAICGVPADKFRTTCSAIDKLDKEPWSNVRREMVEEKDIPETVADRIEPFVC-KVGSPRELHAQLVKEGLFGDNANARHAMNDLALLFDYLDAMGVLDFISFDLSLARGLDYYTGLIYEFVLTSPEHHVGSIAAGGRYDNLVGMFSATDQQIPCVGVSLGIERIFGILQAHADKQAASAAPPS--QVLVASTGGALLLPRLELCKQLWNANISAEVMQTENPKFTKQLHYALERGTPYMVVIGEDELTKDVVKVKDMASKDEVTVARSELVAELLRRGC 923          

BLAST of mRNA_P-fluviatile_contig9.15162.1 vs. uniprot
Match: W4FK51_9STRA (Histidine--tRNA ligase n=10 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FK51_9STRA)

HSP 1 Score: 590 bits (1521), Expect = 7.160e-191
Identity = 423/984 (42.99%), Postives = 546/984 (55.49%), Query Frame = 2

Query:   89 VSVGARKVLPQDVLLVAVHGARVDLHPATL-----DKACPVSC--KAPHGGDTPFELSDGAGDTTADSALSEPAARATIFARIVSLMNG---RSGLRPEVLIFLCDILNNGITPLLAKEGS-----DGPSVA--KAMLGGGSCMFKGE-VRPLVEAATSCGLSPLSMLTAREGKTLVLGRFDRIGLAAMASAAASNAIEIADGVAALACEATRASTAPFDTARFEVRPHRGQIACAERLRLMLEGSK-SVNSKKASTAADDDAATPEAISTIPQYHGPARDAIAAACRAIMTEFNSSDAGPLGVGEDCVQPRDGTQTELTVTFLSEAVGVLISGCRDR-ALALGGSGAAGVGAHGGEGGGEASKSGPEGLLRELQAVLAEEVALSVALL------RXXXXXXXXXXAGPLNVSARPANGGDAPNQASPPGAPGGGGADTTGMSAAQIAKIEAKRXXXXXXXXXXXXXKREKKKAAGLGVGTAELRQLLAPAAAAAATIGADEIFVRIDPFRTGEQALGPLLCAMAERLS--SGGEQRKPKIAKGARDFLPEQQLRIREHAFTAIRNVFKRHGAVEIDTPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARYLALHGVGNIKRFHIAKVYRRDQPNVQLGRFREFYQCDFDIAGNYGLMVPDAEVLSVACEILSSLP-IGNFQIKLNHRRLLDAILDICGCPPEKFRTICSAIDKLDKMDWTDVRQEMVEEKGLAPESAEKIGSFVNGNAGDAKELWAKLTEEKTFGDHPDAAAAMSELELLFKYLDAMGTLKYVSFDLSLARGLTYYTGLIYECVVV-DGGVRVGSIAAGGRYDNLVGMFSASG-QTPCVGVSIGIERVLTIMEKRLQDERGTKPAPSNVQVYIASVGPGLLEERMRVGRLLWSNDISAEYSTADNPKFPKQMNEVLQRGIPFMVILGEKEVQQGVVQLKDMTKHTQEDMRREDLPQALLAAG 2947
            V+VG++K+  ++   VA  GA+V L P  +     D A  V     A   GD PF              LS    RA + +R+  ++     RS    E L FL  +LN  + P      +     D  S+A   A+ G G    K + V  L E   S GLS    L++ E  TL  G      + A   + A     I+D VAAL CEA +AS +PF  A    RPHRG +  A  LR+ML+ SK +V S K  +           I +IPQYHGPARDAIAAA + + T+ N++ +    +G     P    Q+ L+   L  AV  L  G R R AL    S AA V          AS +    L+      L  EV  + ++L      +          A    V+A+ A      ++A               MS AQ AK+  KR             K+  K + G GVG++  R  L        T   D +    +PF    Q     L  +  +LS  SGGE+R+PKIAKGA+DFLP Q + +RE  F  +R VFKRHG VEI+TPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFAR++ALH  GN+KR+ IA+VYRRD P +  GRFREFYQCDFDIAG Y  M+ DAEV+S+  E+L   P +G F+IKL+HR+LLDAIL++CG P +K RT CSAIDKLDK  W  VR EMV+EK +APE A++I  FV    G  K L+A+L  +K FGDHP AA AM EL LL+ YL+AMG L YVSFDLSLARGL YYTGLIYE V+  +   +VGSIAAGGRYDNLVGMFSA+  Q PC+GVS+GIER+  +++K  +   G +   S  QV +     GLL  R+ V + LW+  I+AE   ++NPKF KQ+   L  GIP++V+LGE E     V++K +    +  +  +DL Q L + G
Sbjct:    5 VTVGSKKLSLEEFSSVAFVGAKVALEPLAVVDLHKDDAARVVALPSASSPGDGPF--------------LSPALGRAFVLSRVNFIVKHTLLRSNAILETLDFLVALLNADLIPQFPAPTTKLVDVDAASIALLDALKGHGKATLKSKNVVSLSEGLASVGLSLPVALSSIEQATLTQGGGSLQAVVASIVSGAKGLTPISDAVAALTCEALQASPSPFQEAHDSTRPHRGMLTVATHLRVMLDNSKYAVASDKVDSLV---------IRSIPQYHGPARDAIAAAAKVVETDLNATGSA---LGLSSFHPELLKQSLLS---LHSAVEALFDGSRGRVALLPTSSAAAAVAVES-----LASPAATFALVEATTTQLTGEVRAAKSILAAQEASQGGGGVKGVDSAKAAEVAAKQAAAEAKEDEA------------LAAMSEAQRAKVLEKRRLKADKLKEKAA-KKAAKDSLGAGVGSSAFRAFL--------TASQDYL----NPFDFRVQGAVVFLEDLFAKLSGLSGGEKRRPKIAKGAQDFLPPQ-MHLREQIFHKVRAVFKRHGGVEIETPVFELKETLTGKYGEDSKLIYDLADQGGELLALRYDLTVPFARFMALHNPGNMKRYAIARVYRRDNPQIARGRFREFYQCDFDIAGVYPTMLADAEVISIGIEVLQQFPELGPFKIKLSHRKLLDAILELCGVPADKLRTTCSAIDKLDKEPWDVVRAEMVDEKDIAPEVADRIQGFVT-QVGSPKALYAQLIADKRFGDHPAAAEAMKELGLLWSYLEAMGVLDYVSFDLSLARGLDYYTGLIYEFVLTGESATQVGSIAAGGRYDNLVGMFSATNAQIPCIGVSLGIERIFGMLQKHAEQRDGLRTPVS--QVLVTQTSAGLLVPRLEVCQQLWAAHIAAEILPSENPKFVKQLQHALDAGIPYIVVLGEDERANHQVKVKVLATKEEVTIDEKDLVQTLHSFG 925