mRNA_P-fluviatile_contig82.14815.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: A0A835Z5W8_9STRA (UBA_e1_C domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z5W8_9STRA)

HSP 1 Score: 746 bits (1927), Expect = 9.980e-236
Identity = 559/1526 (36.63%), Postives = 669/1526 (43.84%), Query Frame = 2

Query:  443 IDEDLYSRQLYVMGKTAMAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLLEGPLTDEVIEGGGYAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTISRFEDLRDAKGAPLDTPTQEFTVQNIDPRKF---SVDG----------ASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALRPDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLALVKGSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALPSPLPSLKACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELR------------------------------GQGAFACWQTLRDARL--------------------------------------------------------------------------------------------------DLEDEAPAG--------------FGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQH-------------------------------------------REFVWWASVLRAGVYGLDVPRSIDDLEAASSPSPSTPSKGPKGAPRIISPSSPPPPARES----------------------------------DPDEAIEPLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQGSP--------TTAHKNGFVNLAAPFVAFSEPLEAEPVDGSASGGRG-FTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANFMH-GDHDDPVLDTPLWQLA 4294
            +DE LYSRQLYVMGK AM +MGK+DVLI+GLSGLGAE+AKN+ILAGV+SVTLHD+RP +  DL+S FCL P++V                 + RA  SL  LRELNPYV+VRLL GPL    +  G Y  V  VDET+G+QL  N+A R  G  F+SAS+RGAFAS FCDFGD F V D DG++P A LV  +                            T    VE V  ERH F  GD + R E L     A             ++ R+    SVDG             AEG    +A  GRAVQVK P  ++F PL  AL P  V+ L++P+DF K  P R +TVHACF  LD FRR HGGR P  GSA+DA AFLALV+  P F+                                   GR    D  VA AF+RT AG   PV+ F+GG+VAQEVLK  S LFVP RQFMYYDC EALP+P            R   +RA+      R L  ++V +VGAGAIGCELLK+ ALMGVGC                      G    G               XXXXXXXX       G+D G+                                                                                                                           +VTDMDTIEKSNLNRQFLFR++DVG+AKS AAAAA   MNPAL+V  L K+VG ++E++F + FWE LD   NALDNVEAR+YVD++ V  ARPLLESGTLG +GNTQVVLP  +ESYG+S DPED S+PLCTLKH P  I HT+HWARD FDG F  RP  A R+L A G                 +A AE  ++ EL                               G G+                                                                                                              D    AP+G              F DCL WAK QF +LF    E+L+ QHP+ S D+EGDPFW G +RPP    LDS    H                                           REFVWWA+VLRA VYG+ +PRS+ +L +                     P SP P A                                     D  E     ++A +A  R  A  WR  G               L P  FEKDDD NGHVDF+ AASNLRA +YGIP ADRL TKR+AG+IVPAIATTT+VV+GLA VEL KL   +P            KN FVNLA PFVAFSEP EAE     A   RG FT+WD+  V GA +LT+RGLL  L+ E GAA  + +   +A LYA+F+  G  D+  L   +  LA
Sbjct:   91 LDEALYSRQLYVMGKDAMLRMGKSDVLITGLSGLGAEIAKNVILAGVKSVTLHDERPATWNDLASHFCLSPDSVG----------------KPRAEESLAFLRELNPYVEVRLLRGPLPLGAVAAGAYGVVCAVDETLGAQLALNDAARAGGARFVSASARGAFASAFCDFGDAFTVHDADGQEPRAVLVSRV----------------------------TADGCVEFVRDERHGFDVGDRL-RLEGLEARGAAGXXXXXXXXXAAALEGRELEVVSVDGPFALHVAVAPGGLAEG---AEAVHGRAVQVKAPRRVAFRPLRAALAPPHVDALLVPSDFAKCAPRRALTVHACFLTLDRFRRGHGGRLPRAGSARDAAAFLALVQAHPLFA-----------------------------------GRAWGPDADVARAFARTAAGNFGPVSAFYGGLVAQEVLKAASGLFVPLRQFMYYDCAEALPAP-----------PRARARRAL------RALMRQRVLLVGAGAIGCELLKNFALMGVGC-------------------RSAGDDADGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDDRGV---------------------------------------------------------------------------------------------------------------------------IVTDMDTIEKSNLNRQFLFRARDVGRAKSEAAAAAARAMNPALRVAPLLKRVGRESEDYFGDAFWERLDVAINALDNVEARLYVDARAVRAARPLLESGTLGARGNTQVVLPGLSESYGASADPEDASVPLCTLKHFPSDIAHTIHWARDVFDGFFALRPGAANRVLAAAG-----------------DAAAERALLSELAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGSGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGFTAPNAGGSGYGSSGHGADGYTGGDDYTGGAPSGGDXXXXXXXSGVVEFADCLQWAKAQFRALFVREVEELMAQHPLDSVDEEGDPFWTGARRPPRAPALDSRQPSHSSNHVERLRKWLTADASSASXXXXXLSHSATLTARAAAPRARPREFVWWAAVLRARVYGIPIPRSLSELHSQ--------------------PRSPHPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDAGEEGGEGLEAALAAARAAALEWRARG------------LPALSPLKFEKDDDANGHVDFIAAASNLRAAAYGIPPADRLRTKRVAGRIVPAIATTTAVVAGLACVELAKLAALTPRRGARVPSAAPFKNAFVNLAEPFVAFSEPAEAE----RAPWARGTFTVWDRAAVRGARDLTLRGLLALLRREHGAARTS-VHAGDALLYASFLDPGGEDEAKLSARVVDLA 1320          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: W7UBG5_9STRA (Ubiquitin-activating enzyme e1 n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7UBG5_9STRA)

HSP 1 Score: 634 bits (1635), Expect = 2.650e-196
Identity = 450/1355 (33.21%), Postives = 630/1355 (46.49%), Query Frame = 2

Query:  443 IDEDLYSRQLYVMGKTAMAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLLEGPLTD-----EVIEGGG--YAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTI----------SRFEDLRDAKGAPLDTPTQEFTVQNI-DPRKFSVDGASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALRPDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLALVKGSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALP-------SP------LPSLKACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAF-ACWQTLRDARLDLE--DEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGL-DVPRSIDDL-EAASSPSPSTPSKGPKGAPRIISPSSPPPPARESDPDEAIEPLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQGS--------------------------------PTTAHKNGFVNLAAPFVAFSEPLEAEPVDGSASGGRG--FTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANFMHGDHDDPVLDTPLWQLAD 4297
            +DE LYSRQLYV+G  A  ++  +++L+ GL G+G E+ KN+ILAG +SVTL D  P  + DL++Q+ L   ++                 + RA  S   L+ELNP V + LLE    D     E I   G  +  ++  D+   S+   N   RR    F++A + G F S+FCD GD F V D DG++    L+ +                        +    T   +V + EGERH+   GD +           + +D   A  A  D  T  FTV+ I  P  F+V  ++    +   +   GR  Q+K+PL + F PLT A +  R     +PTDFGK    RQ+T+HACF  L  F+   GGR P PGSA DA AF  LVK              L+   SS  A ++ R+  + P  K++      +DE +   F+RTC G+L+P+  F GG  AQEVLK C+  FVP +Q++++D VE L        SP      LPS K CA+RGD+YDG RAV+G  L + L  + +FVVGAGAIGCELLK+LALMGVG                                                                                                                                                                                             VTD D+IEKSNLNRQFLFR +D+G  KS AAA AV R+NP ++VRAL +KVG +TE FFD++FW   D V NALDNV AR YVD +CV + RPLLESGT GT GN QVV+PF TESYGSS DP + SIP+CTLK  PY+IEH + WARD FDG F+ +P     L                 +R  E+  A A V+  LR Q +     + L    +DLE  D  P+    C+ WA  +F  L+    E LLR+HP  S  ++G+PFW G +R P+P+  D +   H +F+  A++L+A V+ + D   +++ + E   +      + G +    ++          E + +  +E  +Q E  ++R L  +     GG  G G +    R   P  FEKD D NGH+ F+ +ASNLRA +YG+  ADRL TK+IAG+I+PAIATTT+VV+GLA +EL KL Q S                                P +A++N F+NLA PF A ++P+EA P+   A G +G  + +WD++ V    ++T++ LL       G    ++  + +  LYA+FMH   D+P+L   + +L +
Sbjct:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLG----------------QSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSF-----------------------DPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVH-QYSNGRFRQLKQPLQLGFQPLTLARQAMRS----VPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSC----------DVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLS---------ESSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGYLVDLEIWDPVPSARR-CVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVV--------RAEEEEEGDLEAKVQREFEEVR-LTIKRIAKRGGDNGKGTECTAARTR-PEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPI---ALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVE 1159          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: D7FPC4_ECTSI (Ubiquitin activating enzyme E1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPC4_ECTSI)

HSP 1 Score: 619 bits (1596), Expect = 8.030e-192
Identity = 337/529 (63.71%), Postives = 375/529 (70.89%), Query Frame = 2

Query: 2927 QVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAFACWQTLRDARLDLEDEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGLDVPRSIDDLEAASSPSP----------------------------------------------STPSKGPKGAPRIISPSSPPPPARESDPDEAIEPLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQGSPTTAHKNGFVNLAAPFVAFSEPLEAEPVDGSASGGR--GFTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANFMHGDHDD--------PVLDTPLWQLADHLDSEGGGQDWMSRDG 4345
            QVVLPF++ESYGSS DPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKA G               GEEA    VVM+ELRGQGAF+CWQ+LRDARLDLE+E P  FGDCL WAK QF S FHD+AE+LL+QHP+GSTDDEGDPFW GV+RPPSPLKLDS  L HREFVWWASVLRAGVYG++VPRS+ DL+A  + +                                                +P +G +  P           A ESDPDE IEPLI++E+A LRE A                              DDDLNGHVDF+ AASNLRA +YGIP ADRLSTKRIAGKIVPAIATTT+VVSGLA VELLKL+QG+P T HKNGFVNLAAPFVAFSEPLEAEP+DG++ GG   GFTIWDKVVVDGAA+LTVRGL+EFLKSERGAAEV+MISYKNAFLYA+FMHGD DD        PVLDTPLWQLA+ LD++GG QDWMSRDG
Sbjct:  181 QVVLPFQSESYGSSVDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKACGS-------------PGEEA----VVMQELRGQGAFSCWQSLRDARLDLEEEVPETFGDCLQWAKEQFASFFHDSAEELLKQHPLGSTDDEGDPFWTGVRRPPSPLKLDSANLLHREFVWWASVLRAGVYGVEVPRSVRDLQAPVAATAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXREQSPREGRRHRPLAK--------ATESDPDETIEPLIRSELAGLRETA------------------------------DDDLNGHVDFITAASNLRASNYGIPAADRLSTKRIAGKIVPAIATTTAVVSGLACVELLKLIQGAPITDHKNGFVNLAAPFVAFSEPLEAEPIDGASGGGGEGGFTIWDKVVVDGAADLTVRGLVEFLKSERGAAEVSMISYKNAFLYASFMHGDVDDEGEEGDPHPVLDTPLWQLAEALDTDGG-QDWMSRDG 653          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: A0A4D9D8Q8_9STRA (UBA_e1_C domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D8Q8_9STRA)

HSP 1 Score: 632 bits (1630), Expect = 2.700e-189
Identity = 454/1356 (33.48%), Postives = 630/1356 (46.46%), Query Frame = 2

Query:  443 IDEDLYSRQLYVMGKTAMAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLLEGPLTD-----EVIEGGG--YAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTI----------SRFEDLRDAKGAPLDTPTQEFTVQNI-DPRKFSVDGASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALRPDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLALVKGSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALP-------SP------LPSLKACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAF-ACWQTLRDARLDLE--DEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGLDVPRSIDDLEAASSPSPSTPSKGPKGAPRIISPSSPPPPA---RESDPDEAIEPLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQGS--------------------------------PTTAHKNGFVNLAAPFVAFSEPLEAEPVDGSASGGRG--FTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANFMHGDHDDPVLDTPLWQLAD 4297
            +DE LYSRQLYV+G  A  ++  +++L+ GL G+G E+ KN+ILAG +SVTL D  P  + DL++Q+ L   ++                 + RA  S   L+ELNP V + LLE    D     E I   G  +  ++  D+   S+   N   RR    F++A + G F S+FCD GD F V D DG++    L+ +                        +    T   +V + EGERH+   GD +           + +D   A  A  D  T  FTV+ I  P  F+V  ++    +   +   GR  Q+K+PL + F PLT A +  R     +PTDFGK    RQ+T+HACF  L  F+   GGR P PGSA DA AF  LVK              L+   SS  A ++ R+  + P  K+K      +DE +   F+RTC G+L+P+  F GG  AQEVLK C+  FVP +Q++++D VE L        SP      LPS K CA+RGD+YDG RAV+G  L + L  + +FVVGAGAIGCELLK+LALMGVG                                                                                                                                                                                             VTD D+IEKSNLNRQFLFR +D+G  KS AAA AV R+NP ++VRAL +KVG +TE FFD++FW   D V NALDNV AR YVD +CV + RPLLESGT GT GN QVV+PF TESYGSS DP + SIP+CTLK  PY+IEH + WARD FDG F+ +P     L+                +R  E+  A A V+  LR Q +     + L    +DLE  D  P+    C+ WA  +F  L+      LLR+HP  S  ++G+PFW G +R P+P+  D +   H +F+  A++L+A V+ +  P +   +E  S    +T          I++    PP      E + +  +E  +Q E  ++R L  +     GG  G G +    R   P  FEKD D NGH+ F+ +ASNLRA +YG+  ADRL TK+IAG+I+PAIATTT+VV+GLA +EL KL Q S                                P  A++N F+NLA PF+A ++P+EA P+   A G +G  + +WD++ V    ++T++ LL       G    ++  + +  LYA+FMH   D+P+L   + +L +
Sbjct:   38 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLG----------------QSRAMCSFAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSF-----------------------DPLPVTGETLVTLREGERHDLSKGDLVYFERTAAEEDGQTKDAVRAGSAAPDLETDTFTVKRIVSPSCFTVPASANLVKQVH-QYSNGRFRQLKQPLQLGFQPLTLARQAMRS----VPTDFGKASLGRQLTLHACFESLRRFKTHAGGRLPIPGSATDAAAFKKLVKSC----------DVLSDVISSHSAAMAARSAAKRPTSKTKAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGNDESPEIFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLMARLS---------ENSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGCLVDLEIWDPVPSARR-CVKWALDRFHELYQSNIVALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDRSDPLHMDFLLSAALLKARVFNVVDPTTT--VEGMSETVQAT-------LDEILASGYQPPTEVVRAEEEEEGDLEAKVQREFEEVR-LTIKRIAKRGGDNGKGTECAAARTR-PEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEERLSKQRRRHRRHYPPLQAYRNAFINLAGPFLALTQPMEAHPI---ALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVE 1129          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: R7QQ62_CHOCR (Ubiquitin-activating enzyme E1 n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QQ62_CHOCR)

HSP 1 Score: 603 bits (1555), Expect = 1.780e-187
Identity = 435/1305 (33.33%), Postives = 597/1305 (45.75%), Query Frame = 2

Query:  494 MAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLLEGPLTDEVIEGG---GYAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTISRFEDLRDAKGAPLDTPTQEFTVQNIDPRKFSVDGASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALRPDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLALVK---GSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALPSPLPSLKACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAFACWQTLRDARLDLEDEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGLDVPRSIDDLEAASSPSPSTPSKGPKGAPRIISPSSPPPPARESDPDEAIEPLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQGSPT--------------------------TAH-----KNGFVNLAAPFVAFSEPLEAEPVDGSASGGRGFTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANFMHGDHDDPVLDTPLWQLAD 4297
            M  M K+ +LI G  GLG E+AKN+ILAGV+SV ++D  P +L DLSS F L    V+                  RA+A +  L  LNPYV+V + +G L     +      +A V++V+++  +Q   +  C + G  FLSASSRG F S+FCDFG +F+V D  GE P + ++ +I +E                             +V  ++ +RH+ +SGD +S      + +G       +   ++ + P  F+++    +     V+  GG A QVK P+T+SF PL +A       E ++ TDF K+D     TVHACF  +D+     GG  P PGSA+DA  F  L+K   G P  S                                            VA AF+RTCAG +SP+A   GGV AQEVLK  S  F+P RQF+Y+D +E LP+PLPS   CA +G RYDGQ AV G   Q ++ + K F+VGAGAIGCE+LK+ A+MGVG G                                                                    ++GGV                                                                                                                 +VTDMDTIEKSNL+RQFLFR  D+GK KS AAA A++RMNPA+KV A E +VG +TE+ F++DFWESL  V NALDNV+AR+YVD +CV + + LLESGTLGTKGNTQV++P  TESYGSS DP + SIP+CTLK+ PY IEHT+ WARD F+G F+     +   LK                  G+E       M ELR QG      TL      L ++ P    DC++WA+R F  LF+     LL   P  + D  G PFW G KR P+P++ D     H EF+  ++ LRA  YGL V  +        + +   P   PK   +I +         +++    I+    A+ A++ EL  +            ++      + P  FEKDDD N H+DF+ A SNLRA +Y I  ADR  +KRIAGKI+PAIATTT+ V+G+  VEL KLVQ   T                          TA      KNGF NLA PF AFSEP+ A   +     GR +T+WD+  ++   ++++   L+  K +R   E++MIS   + LY++F         +  PL + A+
Sbjct:    1 MRAMQKSALLIVGCDGLGVEIAKNVILAGVKSVAVYDPHPAALPDLSSNFYLTEAHVSAKT--------------SRAKACIKDLVSLNPYVNVSVYDGMLNLNEPDRNVFSSFAVVVMVNQSHAAQRAVDSVCHKAGVKFLSASSRGVFGSIFCDFGPSFIVTDPTGEPPRSVMISSITKENPP--------------------------IVTCLDEQRHDLESGDYVS----FTEVQGMVELNDGKPHKIKVLGPYTFALEETDISSFANYVR--GGYATQVKMPVTVSFKPLGDAFASP---EYVI-TDFAKMD--HMDTVHACFAAVDELP---GGSLPRPGSAEDAATFTKLLKLRDGEPIAS--------------------------------------------VAEAFARTCAGNISPIAAGLGGVAAQEVLKAISGKFMPIRQFLYFDSMECLPNPLPSEADCAPKGSRYDGQIAVFGAKFQEKIENLKYFLVGAGAIGCEMLKNFAMMGVGVG--------------------------------------------------------------------QNGGV-----------------------------------------------------------------------------------------------------------------IVTDMDTIEKSNLSRQFLFRDTDIGKPKSIAAAGAILRMNPAMKVVAHEIRVGPETESTFNDDFWESLSGVCNALDNVQARLYVDQRCVYYMKSLLESGTLGTKGNTQVIVPHMTESYGSSRDPPEKSIPICTLKNFPYRIEHTLQWARDYFEGEFKTSAEESNSFLKR-----------------GQE------YMDELRRQGPGTMLATLEMLNACLLEDRPNSVQDCVEWARRAFEKLFNGEIRQLLHTFPRDNVDKNGVPFWSGTKRAPNPVEFDVNNEMHSEFIIASACLRAENYGLRVSHADIAQMGKQASAVMIPEFVPKSGVKIAT--------TDAEAKAQIDNPGDADDARIEELVTKLLH---------EKSLAGFQMTPLEFEKDDDSNHHIDFITACSNLRATNYSIETADRHQSKRIAGKIIPAIATTTAFVTGMVCVELYKLVQIGVTDIAQNKDDGAWARPNSDAYSAENEKATAKTLELFKNGFANLALPFFAFSEPMAAPKTE--MGHGRKWTLWDRFDINEGRDISLEEFLKLFK-DRFGLEISMISCGVSMLYSSFTSAAKLRERMPMPLSKAAE 982          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: A0A7S1ETX1_9RHOD (Hypothetical protein n=1 Tax=Timspurckia oligopyrenoides TaxID=708627 RepID=A0A7S1ETX1_9RHOD)

HSP 1 Score: 600 bits (1546), Expect = 7.220e-186
Identity = 438/1302 (33.64%), Postives = 594/1302 (45.62%), Query Frame = 2

Query:  443 IDEDLYSRQLYVMGKTAMAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLL-EGPLTDEVIEGGGYAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTISRFEDLRDAKGAPLDTPTQEFTVQNIDPRKFSVDGASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALRPDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLALVKGSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALPSPLPSLKACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAFACWQTLRDARLDLEDEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGLDVPR-SIDDLEAASSPSPSTPSKGPKGAPRIISPSSPPPPARESDPDEAIE---PLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQGSPTTAHK-------------------------------NGFVNLAAPFVAFSEPLEAEPVDGSASGGRGFTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANF 4240
            IDE LYSRQLYV+G  A  +M  ++V I GL+GLG E++KN+ LAG++++T+ DD      DLSS F    E+  G +               RA+A+  HL  LNPYV V +L E  LTDEV+    Y  V+ VD+ + +Q RAN+ CR  G  F+S SSRG F  +F DFGD F+V D  GE+P + L+ AI +                      D+  T    V  ++  RH+F++GD +S      + +G       +   ++   P  FS++ +      +  +  GG A +VK P+T+SF PL +AL     E   L TD+   DP   M  H  F  LD +R E  G+FPTPGSAKDA  F+AL+K                                               D A+   FSR CAG+++P+A   GG+VAQEVLK  S  F+P +QF+Y+ C++ALP PLPS   CA RG+RYD Q A+ G   Q +L + + F+VGAGAIGCE+LK+ ALMGVG                                                                                                                                                   T  TG                                     VTDMD IEKSNL+RQFLFR  D+G AKS AAA AV  MNP + + +  ++VG +TE  F++DFW++L  V NALDNV+AR+YVD +CV F + LLESGTLGTKGN QVV+PF TESYGSS DP + S+P+CTLK+ PY IEHT+ WARD F+G F+A P   +                      G+E       + ELR QG       L   + DL    P    DC+ WA+  F  LF  + + LL   P    D  G PFW G KR P+P+K D +   H +F+   ++LRA VYG+     S +DL+A +      P   PK   +  +          SD +   +     +  E   + EL     +P      D D  +    + P  FEKDDD N H+DF+ A SNLRA +Y I  ADR  +K IAGKI+PAIATTT+ ++GL  +ELLKLV+   T   K                               N F NL  PF +F+EP+ A   +    GGR +T+WD V VD   ++T++  L   + ER   E++M+S   + LY++F
Sbjct:   12 IDEALYSRQLYVLGHDAQRRMAASNVCIWGLTGLGVEISKNLTLAGIKTLTIVDDEIVQWADLSSNF-YAHESDVGKV--------------SRAQAASQHLVTLNPYVAVHVLKEAELTDEVL--ATYQVVVCVDQMLETQKRANKVCRAAGGKFVSTSSRGVFGQIFNDFGDEFIVLDPTGEEPPSTLISAITK----------------------DSSPT----VSCLDDHRHDFETGDFVS----FTEVRGMTEINDGKPRKIKVTGPYTFSLEDSEDVMDFSDYER-GGYATKVKMPVTLSFKPLEQALS----EPEFLMTDY---DPPTAM--HVAFQTLDKYRVEREGKFPTPGSAKDAAEFVALMKALD----------------------------------------AENADAALGEMFSRCCAGSMNPMAATLGGIVAQEVLKAISFKFMPIKQFLYFKCIQALPEPLPSEDDCAPRGNRYDAQIAIFGHSFQHKLKNLRYFLVGAGAIGCEMLKNWALMGVG---------------------------------------------------------------------------------------------------------------------------------------------------TSDTGK----------------------------------VYVTDMDQIEKSNLSRQFLFRDSDIGSAKSTAAANAVKAMNPRMNIESFLERVGPETEEKFNDDFWDNLSGVCNALDNVQARLYVDQRCVYFKKSLLESGTLGTKGNVQVVVPFLTESYGSSRDPPEKSVPICTLKNFPYQIEHTLQWARDYFEGAFRAGPEEIKNYATR-----------------GDE------FIEELRKQGPGTMVGVLETLKKDLITSKPESLQDCVAWARLVFEELFSSSIQQLLIAFPHDMLDSNGIPFWSGTKRAPNPIKFDISNPMHFDFIEAGALLRAQVYGISTENVSTEDLKAMTLKV-DVPEFKPKTGMKFAT----------SDAEAETQGGASAVDHEDTIVEELIKS--LP------DPDSLKESMNVSPIEFEKDDDSNHHMDFITACSNLRATNYSIEPADRHKSKLIAGKIIPAIATTTAFITGLVCIELLKLVKLGYTDVKKESVQNAWAIEGSGSVSDEKKKEDARRLEIFQNSFCNLTLPFFSFAEPMPAPKTE--IPGGRAWTLWDCVEVDEGRDITLKEFLSIFE-ERYGWEISMLSCGVSILYSSF 990          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: UPI000C20CAD5 (ubiquitin-like modifier-activating enzyme 1 isoform X1 n=2 Tax=Onthophagus taurus TaxID=166361 RepID=UPI000C20CAD5)

HSP 1 Score: 597 bits (1538), Expect = 1.220e-184
Identity = 437/1290 (33.88%), Postives = 592/1290 (45.89%), Query Frame = 2

Query:  443 IDEDLYSRQLYVMGKTAMAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLLEGPLTDEVIEGGGYAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTISRFEDLRDAKGAPLDTPTQEFTVQNIDPRKFSV-DGASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALR-PDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLALVKGSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALP---SPLPSLKACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAFACWQTLRDARLDLEDEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGLDVPRSIDDLEAASSPSPSTPSKGPKGAPRIISPSSPPPPARESDPDEAIEPLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQGSPT-TAHKNGFVNLAAPFVAFSEPLEAEPVDGSASGGRGFTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANFMHGDHDDPVLDTPLWQLA 4294
            IDE LYSRQLYV+G  AM +M  +DVLISGL GLG EVAKN+IL GV+SVTLHD+   + +DLSSQF L  + +                 + RA A +  L ELN YV  +   GPLT+E I+   +  V+L + ++  QL+  E     G A + AS+RG FA +FCDFG +F V D +GE P++ ++  + R+++                           VV  ++  RH  + GD ++  E +    G       +   ++ + P  FS+ D +S+ + E      GG A QVK P  + F PL+E+++ PD V      TDF K D  +Q+  H  F  L  F  E  GR P P S +DA  FL L K                         VS   G              E++  +   FS  CAG L+P+    GG+VAQEV+K CS  F P  Q++Y+D +E LP   +P+    A AA G RYD Q AV G   Q++L + K FVVGAGAIGCELLK+ A+MGVG                                     GG++                                                                                                                                                    VTDMD IEKSNLNRQFLFR  DV +AKSA AA  + +MNP + + A E +VG +TE+ +D+ F+E LD V NALDNV+AR+Y+D +CV + +PLLESGTLGTKGNTQVV+PF TESY SS DP + SIP+CTLK+ P  IEHT+ WARD F+GLF+    NA + LK                           V R L+  G     + L   +  L DE P  F DC++WA+  +   + +  + LL   P       G  FW G KR P  L  D     H ++++ A+ L+A VYG+   R    ++   S     P   PK   +I    S    +  S+ D      +Q E+  + EL +                     L P  FEKDDD N H+DF++AASNLRA +Y IP ADR  +K IAGKI+PAIATTTSVV GL  +EL KLV    + T  KNGFVNLA PF  FSEP+EA  ++     G+ +T+WD+  + G  E+T+   L++ K+E G  E+ M+S     LY+ FM  +     L  P+ ++ 
Sbjct:   55 IDESLYSRQLYVLGHDAMRRMATSDVLISGLGGLGVEVAKNVILGGVKSVTLHDETECTRQDLSSQFYLSEDDIG----------------KNRAEACVKQLAELNTYVPTKTYTGPLTEEWIKK--FRVVVLTNSSISEQLKIGEMTHSLGIALIIASTRGLFAQVFCDFGPSFTVLDVNGESPVSAMIADVSRDKES--------------------------VVTCIDDTRHGMEDGDYVTFSEVI----GMTELNNCEPIKIKVLGPYTFSIGDTSSYTKYER-----GGIATQVKMPKKVQFKPLSESIKSPDFVV-----TDFAKFDHPQQL--HLAFSTLHKFT-EKNGRLPKPWSFEDASEFLTLAKS------------------------VSVDGG-----------NDTEINNELLETFSNICAGDLNPMNATIGGIVAQEVMKACSGKFHPIYQWLYFDAIECLPRNGNPINETLA-AATGSRYDAQIAVFGSEFQKKLGALKYFVVGAGAIGCELLKNFAMMGVGS-----------------------------------DGGKI---------------------------------------------------------------------------------------------------------------------------------------------------TVTDMDLIEKSNLNRQFLFRPHDVQRAKSATAAKVIKKMNPLINIVAHENRVGPETESIYDDTFFEDLDGVANALDNVDARIYMDRRCVYYRKPLLESGTLGTKGNTQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDNFEGLFKQNAENASQYLKD-----------------------REFVERTLKLPGVQPI-EVLESVKTALVDERPLSFQDCVEWARNHWQEQYSNQIKQLLFNFPPDQLTSTGQLFWSGPKRCPEALTFDIDNSLHMDYIYTAANLKAEVYGIPQVRDYKVVKEMVSKI-QVPEFIPKSGVKIAVTDSQMAVSNGSNVDMDRVAQLQLELPTVNELGSLM-------------------LTPLEFEKDDDKNLHIDFIVAASNLRAANYKIPSADRHKSKLIAGKIIPAIATTTSVVGGLVCLELYKLVNNMKSITPFKNGFVNLALPFFGFSEPIEAPKMEYC---GKPWTLWDRFEIQG--EMTLSEFLDYFKNEHGL-EITMLSQGVCMLYSFFMAKNKAQERLGLPMSEVV 1015          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: A0A5J4Z7I3_PORPP (Ubiquitin-like modifier-activating enzyme 1 n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4Z7I3_PORPP)

HSP 1 Score: 598 bits (1542), Expect = 5.370e-184
Identity = 469/1330 (35.26%), Postives = 607/1330 (45.64%), Query Frame = 2

Query:  443 IDEDLYSRQLYVMGKTAMAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLLEGPLTDEVIEG--GGYAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTISRFEDLRDAKGAPLDTPTQEFTVQNIDPRKFSV-----DGASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALRPDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLA-LVKGSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALPSPLPSLKACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAFACWQTLRDARLDLEDEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGL-DVPRSIDDLEAASSPSPS----------------TPSKGPKGAPRIISPSSPPPPARESDPDEAIEPLIQAEMAKLRELAA--RWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQ------GSPTTAH-------------------------------KNGFVNLAAPFVAFSEPLEAEPVDGSASGGRGFTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANF 4240
            IDE LYSRQLYV+G  A  +M +++VLI G+SGLG EVAKN+ILAGV++VT+ DD P +  DLSS F   P  VA  +               RA A L  L  LNPYV+V   +  L D+ ++     +  V+ VD+ +   L  + A R  GT F + SSRGAF  +F DFG  F V D  GE P + L+ +I +E+    ++ +TG            K+     V  +E  RH+F++GD ++ F +++   G    TP +   ++   P  F +     D A   +GE      GG A QV  P T+SF+PL EAL  D  E L+  +DF K D  R  T+HACF  LD F    GG  P PGS+ DAEAF+A L K  P                                        G  ++  VA AF+RTCAG LSP+A   GG+VAQE LK  S  F P RQF Y+DC EALP PLPS   CA    RYDGQ AV+G     R+ + + F+VGAGAIGCE+LK+ ALMG+G G                                    GRV                                                                                                                                                   +VTDMDTIEKSNL+RQFLFR  D+G  KS AAA AV  MN  + V + E +VG +TE  FD+ FWE L  V NALDNV+AR+YVD +CV + +PLLESGTLGTKGN QVV+P  TESYGSS DP + SIP+CTLK+ PY IEHT+ WARD F+G F+A P +A                     R G+E       + +LR QG      TL +   +L    P  F DC+ WA+  F  LF  + + LL   P    D  G PFW G KR P+PL+ D     H  F+  A+ LRA VYG+ DVP S   L AA+  +                   P+  PK   +I +  +      ES     I    + E+ KL +        V G G  G+  Q      L P  FEKDDD N H+DF+ A SNLRA +Y I  A+R  +K IAGKI+PAIATTT+ V+GL  +EL KLV+      GS  ++                                KNGF NLA PF  FSEP+ A     S   GR +T+WD+  VD   ++T+   L   + ER   E++MIS   + LY++F
Sbjct:   70 IDEALYSRQLYVLGHDAQKRMAESNVLIVGMSGLGVEVAKNLILAGVKTVTIADDTPVAWVDLSSNFYCEPADVAAGV--------------SRAAACLPKLVTLNPYVNVS--QTTLHDDQVDAEIAKHQVVICVDQPLARCLLVSAAARSAGTKFTACSSRGAFGMIFNDFGAEFTVLDPTGEPPRSVLLSSIFKEK----STDDTG------------KEKPVLSVTCMEEHRHDFETGDFLT-FSEVKGLVGLNDGTPRK---IRVTGPYTFKIELDEHDDALVLQGEYDR---GGVATQVVMPQTLSFLPLGEAL--DAPEFLV--SDFAKFD--RLHTLHACFRSLDAFAAARGGAVPVPGSSADAEAFIASLSKCLP---------------------------------------EGEPLNRDVAQAFARTCAGNLSPMAATLGGIVAQEALKAISFKFGPIRQFFYFDCTEALPEPLPSEAECAPLQCRYDGQIAVLGRSFVDRIRALRYFLVGAGAIGCEMLKNWALMGIGSGA----------------------------------DGRV---------------------------------------------------------------------------------------------------------------------------------------------------LVTDMDTIEKSNLSRQFLFRDSDIGAPKSGAAARAVQAMNAEMNVESFEDRVGPETEEKFDDAFWEGLSGVCNALDNVQARLYVDQRCVYYCKPLLESGTLGTKGNVQVVVPHLTESYGSSRDPPEKSIPICTLKNFPYQIEHTLQWARDYFEGAFRAGPEDANSYA-----------------RRGQE------FIDDLRKQGMGTMLPTLDELHKNLVVARPESFADCVAWARLVFEELFSTSIKQLLFAFPHDMVDSAGIPFWSGTKRAPTPLEFDIHNETHVMFIESAARLRAFVYGIGDVPASTSALTAAAVVNGGGAAFAEVLQPMLERVRIPTFVPKRGMKIATTDA----EAESQGGATITDSDEQEVVKLIDSLPPPESLVAGSGALGEKGQL-----LTPVQFEKDDDTNFHIDFITACSNLRAANYSIEPANRHKSKMIAGKIIPAIATTTAFVTGLVCIELYKLVKLGYTDLGSNKSSKNAWAVPCGDSEEXXXXXXXXXXXAARRLEMFKNGFSNLALPFFGFSEPIAAPK--SSMGSGREWTLWDRFDVD-EGDMTLAEFLAMFE-ERFGLEISMISCGLSMLYSSF 1098          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: A0A2D5NI85_9BACT (E1 ubiquitin-activating enzyme n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2D5NI85_9BACT)

HSP 1 Score: 592 bits (1527), Expect = 1.530e-182
Identity = 430/1284 (33.49%), Postives = 584/1284 (45.48%), Query Frame = 2

Query:  443 IDEDLYSRQLYVMGKTAMAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLLEGPLTDEVIEGGGYAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTISRFEDLRDAKGAPLDTPTQE-FTVQNIDPRKFSVDGASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALRPDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLALVKGSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALPSPLPSLK--ACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAFACWQTLRDARLDLEDEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGLDVPRSIDDLEAASSPSPSTPSKGPKGAPRI-ISPSSPPPPARESDP----DEAIEPLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQGSPTT-AHKNGFVNLAAPFVAFSEPLEAEPVDGSASGGRGFTIWDKVVVDGAA-----ELTVRGLLEFLKSERGAAEVAMISYKNAFLYANFMHGD 4252
            IDE LYSRQLYV+G  AM KM  +DVLISG+ GLG E+AKN+IL GV+SVT+HD     + DLSSQF L    V                 + RA + L  L ELN YV V    G LTD+ +    +A V+L +  +  QLR ++     G   + A +RG ++ +FCDFGD FVV D +GE PL+ ++ +I R+                           + VV  ++  RH F+ GD++       + KG  +D   ++ F ++ + P  FS+   S   G     A GG A QVK P  +SF PL +A      E   L  DF K D   +M  H  FF + +F  +HGGR P P S  DAEAF+   KG                                     S +G   ++D      F++ CAG +SP+    GG+ AQEV+K CS  F+P +Q++Y+D +E LP     L    C A   RYDGQ AV G   Q  +  +K FVVGAGAIGCELLK+ A+MG+G                                                                                                                                                                                            +VTDMDTIEKSNLNRQFLFR  D+ K KS  AA  + +MNP +KV A + +VG +TE+ F +DF+E LD V NALDNV+ARMY+D +CV + +PLLESGTLGTKGNTQVVLP  TESY SS DP + SIP+CTLK+ P  IEHT+ WARD F+G++     NA + L+                      E    V R  + QGA +  +T+   +  L D  P+ F DC+  A+  F   F +    LL   P       G  FW G KR P  +K DS    H ++V  A+ LRA  YGL   R    ++A      + P   PK   +I ++ +     + + D     +E +E L+ +E++K                           + PA FEKDDD N H+DF+ A SNLRA +YGI VADR  +K IAG+I+PAIATTTS+V+GL  +EL KL+QG      +KNGFVNLA PF AFS P+EA       +    +T+WD+ +V GA      E+T++  +++ K+E    E+ M+++  + LY+ FM  D
Sbjct:   88 IDESLYSRQLYVLGHEAMQKMQSSDVLISGMGGLGVEIAKNVILGGVKSVTIHDAGKVEIGDLSSQFYLSEGDVG----------------KNRAESCLSQLAELNSYVPVTAHTGELTDDFVRD--FAVVVLTNSPLEEQLRVSKVTHPHGIRLIVAETRGLYSQVFCDFGDNFVVSDTNGEQPLSAMISSITRD--------------------------AQGVVTCLDEVRHGFEDGDSVK----FTEVKG--MDEVNEKVFKIKVLGPYTFSIGDTS---GFASDYASGGIATQVKVPTNVSFAPLEKAA----AEPEFLLADFAKFDAPAKM--HLAFFAMHEFMAKHGGRLPAPWSNDDAEAFVEAAKGV------------------------------------SMIGTIEDLDADYLRQFAKICAGNVSPMNAAVGGIAAQEVMKACSGKFMPIKQWLYFDALECLPEDRSKLTEAGCKALNTRYDGQAAVFGHDFQAHISKQKFFVVGAGAIGCELLKNFAMMGLG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KMIVTDMDTIEKSNLNRQFLFRPWDIQKLKSQTAANVITKMNPGVKVEARQDRVGPETEDVFTDDFFEELDGVINALDNVDARMYMDRRCVYYKKPLLESGTLGTKGNTQVVLPHVTESYSSSRDPPEKSIPICTLKNFPNAIEHTLQWARDQFEGMYTQAQENAAQYLR----------------------EPAKFVERTRKLQGAQSL-ETMESVKAVLIDRKPSCFEDCVAGARLSFEEFFSNTIRQLLFNFPKDQLTSSGALFWSGPKRCPEAIKFDSNNDLHLDYVVAAANLRAFNYGLPPNRDRAFIKAFVDKV-TVPEFVPKSGVKIAVTDAEAQAQSNQGDSTMGDNETLEELV-SELSKTSSSHP-------------------TKIHPADFEKDDDTNFHMDFITACSNLRATNYGIAVADRHKSKLIAGRIIPAIATTTSLVAGLVGLELYKLLQGHKNVDLYKNGFVNLALPFFAFSTPIEAPKTKYYETE---WTLWDRFLVKGAKPEGGDEMTLQEFMDYFKNEHNL-EITMLTHGVSMLYSFFMQAD 1042          

BLAST of mRNA_P-fluviatile_contig82.14815.1 vs. uniprot
Match: A0A1X6NP78_PORUM (UBA_e1_C domain-containing protein n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6NP78_PORUM)

HSP 1 Score: 591 bits (1524), Expect = 2.720e-182
Identity = 448/1330 (33.68%), Postives = 594/1330 (44.66%), Query Frame = 2

Query:  443 IDEDLYSRQLYVMGKTAMAKMGKADVLISGLSGLGAEVAKNIILAGVRSVTLHDDRPTSLEDLSSQFCLGPEAVAGAMXXXXXXXXXXXXXEGRARASLDHLRELNPYVDVRLLEGP----LTDEVIEGGG-YAAVLLVDETVGSQLRANEACRRTGTAFLSASSRGAFASLFCDFGDTFVVEDRDGEDPLACLVGAIVREEKRKTASAETGGGGRQESGREDAKQTTRWVVEVVEGERHEFQSGDTISRFEDLRDAKGAPLDTPTQEFTVQNIDPRKFSVDGASWAEGETQVKACGGRAVQVKKPLTISFVPLTEALRPDRVEELILPTDFGKLDPSRQMTVHACFFCLDDFRREHGGRFPTPGSAKDAEAFLALVKGSPFFSPPPXXXXXLASTPSSFPAGVSPRAGGRGPVGKSKVGRGLEVDEAVAMAFSRTCAGALSPVAGFFGGVVAQEVLKGCSSLFVPTRQFMYYDCVEALPSPLPSLKACAARGDRYDGQRAVIGDVLQRRLFSRKVFVVGAGAIGCELLKSLALMGVGCGGVDGGVXXXXXXXXXXXXXEEGARFRGDSPAYNDKGGRVGAGXXXXXXXXEEIESLWGEDEGLDGVRLKSGGVGXXXXXXVVDGGGDDGVGXXXXXXXXXXXXXDEGEKKNKARGWLERLKLGPAAEDTTAAVRRPGASSGTAPPTYATGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVTDMDTIEKSNLNRQFLFRSKDVGKAKSAAAAAAVVRMNPALKVRALEKKVGADTENFFDEDFWESLDCVFNALDNVEARMYVDSKCVDFARPLLESGTLGTKGNTQVVLPFRTESYGSSTDPEDGSIPLCTLKHHPYLIEHTVHWARDTFDGLFQARPRNAERLLKAFGGGXXXXXXXXXXYRVGEEAEAEAVVMRELRGQGAFACWQTLRDARLDLEDEAPAGFGDCLDWAKRQFVSLFHDAAEDLLRQHPVGSTDDEGDPFWGGVKRPPSPLKLDSTCLQHREFVWWASVLRAGVYGLDVPRSIDDLE--AASSPSPSTPSKGPKGAPRIISPSSPPPPARES------DPDEAIEPLIQAEMAKLRELAARWRVPGGGGTGDGDQRRWRRPLVPAAFEKDDDLNGHVDFVMAASNLRALSYGIPVADRLSTKRIAGKIVPAIATTTSVVSGLAVVELLKLVQ----------------------GSPTTAH-----------KNGFVNLAAPFVAFSEPLEAEPVDGSASGGRGFTIWDKVVVDGAAELTVRGLLEFLKSERGAAEVAMISYKNAFLYANFMHGDHDDPVLDTPLWQLA 4294
            IDEDLYSRQLYV+G  A  +M  + VL+ G+ G+G E+AKN+ILAG++SVTL D  P    DL++QF L P+ VA                  RA ASL  L  LNPYV V LLE      + D V      Y AV+LV++++ +Q   + ACR     F+S  + GAF  +F DFG  F V D +GE P + ++ A+  +                    +D       VV  ++ +RH+F++GD +S F +++   G     P +   ++ + P  FS+ G             GG A QVK P TI F  L EA      E   +  DF K+   +  T HA F  +D FR   GG  PTPGSA  A  F+  V+ +                     AG +                  ++D  V   F+RT AG LSP+A   GG+ AQEVLK  S  F+P +QF Y DC E LPSP P+   CA  G RYDGQ AV+G  +Q++L +   F+VGAGAIGCE+LK+ A+MGVG     GG                                                           G+                                                                                                                        V+TDMD IEKSNL+RQFLFR  D+ K KS AAAAAV  MN  + + A E +VG +TE  FD++FW+ L  V NALDNV+AR+YVD +CV + +PLL+SGTLGTKGNTQVV+P  TESY SS DP + SIP+CTLK+ PY IEHT+ WARD F+G F+  P +    L + G                           +L+ QG      TL      L  E P    DC+ W++  F  +F  + + LL   P    D    PFW G KR P+PL  ++    H  FV  A++LRA VYG  +P S  D+    A+  +   P   PK   +I +  +       S        DE IE L++A              P G    DG  +     LVP  FEKDDD N H+D + A SNLRA +Y I  ADR  +K IAGKI+PAIATTTS V+GL  +E+LKLV+                      GS   A            KNGF NLA PF AFSEP+ A   D     GR +T+WD + V+   ++T+   LE +K+  G  EV+M+S   + LYA+F +       L TPL ++A
Sbjct:   22 IDEDLYSRQLYVLGHEAQRRMASSSVLLVGVDGVGVEIAKNLILAGLKSVTLFDPMPAMTADLAAQFYLTPDHVAAGTP--------------RAAASLPLLESLNPYVSVSLLEADGGASVADTVSAAASQYTAVVLVNQSLAAQTAVDAACRSADVKFMSVGAFGAFGRIFNDFGPAFTVSDVNGEQPRSVMISAVTVDG-------------------DDV------VVTCLDEQRHDFEAGDHVS-FTEVQGMTGLNDGKPRE---IKVLGPFTFSLVGCREEVAAMGEYVRGGYATQVKMPSTIVFKSLPEAAE----EPEYVIADFAKM--MQMGTTHAAFQAVDAFRATLGGALPTPGSAAHAAKFVEFVEAA---------------------AGSAAD----------------DLDAKVLECFARTAAGVLSPMAATLGGIAAQEVLKATSGKFMPIKQFFYIDCQEVLPSPPPTEAECAPTGSRYDGQVAVLGATVQKKLAALNYFLVGAGAIGCEMLKNWAMMGVGVDAASGG-----------------------------------------------------------GI------------------------------------------------------------------------------------------------------------------------VITDMDQIEKSNLSRQFLFRDADIHKPKSVAAAAAVRVMNSDVNIVAHEVRVGPETEATFDDEFWDGLSGVCNALDNVQARLYVDQRCVYYVKPLLDSGTLGTKGNTQVVVPGLTESYSSSRDPPEKSIPICTLKNFPYQIEHTLQWARDWFEGSFRGTPEDCNAYLGSRG----------------------KDFFDDLKKQGPGTMLATLEAVHSSLVTERPLSVEDCIKWSRLLFEEMFSSSIKQLLHAFPADMVDANKVPFWSGTKRVPTPLTFNAADETHMAFVTSAALLRAQVYG--IPVSDKDMAHFPAALAAVMVPDFVPKAGVKIATTEAEASEQNASGAGSLDQDDERIETLLKALP------------PAGSAGSDGASKT----LVPLEFEKDDDTNHHIDVITACSNLRAANYSIEPADRHKSKMIAGKIIPAIATTTSFVTGLVCIEMLKLVRLGYTDRDGGGYDGGAWCKAAAKGSDAKARRDAAEKKLECFKNGFANLALPFFAFSEPVVAAATD--MGHGRFWTLWDLLDVNEGRDITLGEFLEAIKTHYGL-EVSMVSCGVSILYASFTNPRRLQERLATPLSEVA 1043