prot_P-fluviatile_contig58.12223.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: A0A6H5KEF6_9PHAE (2-isopropylmalate synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KEF6_9PHAE)

HSP 1 Score: 1005 bits (2599), Expect = 0.000e+0
Identity = 545/670 (81.34%), Postives = 583/670 (87.01%), Query Frame = 0

Query:    5 MRGLVGKAACCGCVVAATAGTAAGAVMQVRHGEATAPAAFISAPLIPKAAAAASQQQSRTGFLSAPSGAAGRRQRATADAAVGPVRMVATDPGLATGAAPPGQKAGEAGGMPPL---ARVNVEPSRHDKETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIRDANIRRFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILGTQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRGQPDKAHPQKDV 671
            MRG +G+AAC                M VR+G+A A AAF+SA   P   A   Q  S     +     AGRRQR  + A    VRMVATDPG AT                     ARVNV+PSRHD ETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVG+GDNPPIICGLARAL KDID+CA AV+ ARFPRIHTFIATSDIHM+HKLKK+REQVL+ITREMVTHARSHV+DVEFSAEDALRSD+DFLSEVYSVAIEAGATTINVPDTVGYTTPKEF+GL+HHLR TVRG++ VTISVHGHDDLGMAVANFMSAIEGGARQ VECTINGIGERAGNAALEEIVMALHVRKAYYNPSF RPVLSE  LTNI T+EIV+SSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGL+QDTSLVLGKHSGRHAFRSRLQE+GYSLTDDELNRAFLRFKDLADKKKEI+SLDLASIVNDEIRD NIRR+ELVGM VAAGT NKPTAT+TLYD++  KE CVAAIGTGPVDAAYKAIDDI+GT++T+L+EYSVASVTAGIDALGEVTVRV+DELS+REYIGKAADTDVIHASAEAYLHA+NRLLS+RGQPDKAHPQKDV
Sbjct:    1 MRGFMGRAACXXXXXXXXXXXXXXXXMPVRNGKAAA-AAFVSAQ--PTLEAGRQQAWSNPWVTNY----AGRRQRVASAA----VRMVATDPGTATAEIKXXXXXXXXXXXXXXXXXARVNVQPSRHDIETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGQGDNPPIICGLARALRKDIDVCANAVAPARFPRIHTFIATSDIHMKHKLKKSREQVLEITREMVTHARSHVDDVEFSAEDALRSDYDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFMGLMHHLRGTVRGVDKVTISVHGHDDLGMAVANFMSAIEGGARQ-VECTINGIGERAGNAALEEIVMALHVRKAYYNPSFDRPVLSETALTNINTKEIVKSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLYQDTSLVLGKHSGRHAFRSRLQESGYSLTDDELNRAFLRFKDLADKKKEITSLDLASIVNDEIRDVNIRRYELVGMQVAAGTTNKPTATLTLYDTDLEKEGCVAAIGTGPVDAAYKAIDDIVGTKSTKLLEYSVASVTAGIDALGEVTVRVQDELSKREYIGKAADTDVIHASAEAYLHAVNRLLSNRGQPDKAHPQKDV 658          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: D8LHU2_ECTSI (2-isopropylmalate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHU2_ECTSI)

HSP 1 Score: 919 bits (2374), Expect = 0.000e+0
Identity = 471/516 (91.28%), Postives = 499/516 (96.71%), Query Frame = 0

Query:  157 MTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIRDANIRRFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILGTQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRGQPDKAHPQKDVL 672
            MTREEKLMVAKQL KLGVDIIEAGFPIASQGDFEAVQEIATVVG+G NPPIICGLARAL KDID+CA AV+ ARFPRIHTFIATSDIHM+HKLKK+REQVL+ITREMVTHARSHV+DVEFSAEDALRSD+DFLS+VYSVAIEAGATTINVPDTVGYTTPKEF+GL+HHLR TVRGI+ VTISVHGHDDLGMAVANFMSAIEGGARQ VECTINGIGERAGNAALEEIVMALHVRKAYYNPSF RPVLSE  LTNIKT+EIV+SSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEI+SLDLASIVNDEIRD NIRR+ELVGM VAAGT NKPTATVTLYD++  KE CVAAIGTGPVDAAYKAIDDI+GT++T+L+EYSVASVTAGIDALGEVTVRV+DELS+REYIGKAADTDVIHASAEAYLHA+NRLLS+RGQPDKAHPQKDV+
Sbjct:    1 MTREEKLMVAKQLGKLGVDIIEAGFPIASQGDFEAVQEIATVVGQGGNPPIICGLARALKKDIDVCANAVAPARFPRIHTFIATSDIHMKHKLKKSREQVLEITREMVTHARSHVDDVEFSAEDALRSDYDFLSKVYSVAIEAGATTINVPDTVGYTTPKEFMGLMHHLRGTVRGIDKVTISVHGHDDLGMAVANFMSAIEGGARQ-VECTINGIGERAGNAALEEIVMALHVRKAYYNPSFDRPVLSETALTNIKTKEIVKSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEITSLDLASIVNDEIRDVNIRRYELVGMQVAAGTTNKPTATVTLYDTDLEKEGCVAAIGTGPVDAAYKAIDDIVGTRSTKLLEYSVASVTAGIDALGEVTVRVQDELSKREYIGKAADTDVIHASAEAYLHAVNRLLSNRGQPDKAHPQKDVV 515          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: A0A835YKD7_9STRA (2-isopropylmalate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YKD7_9STRA)

HSP 1 Score: 783 bits (2022), Expect = 2.200e-278
Identity = 410/563 (72.82%), Postives = 476/563 (84.55%), Query Frame = 0

Query:  117 PLARVNVEPSRHDK--ETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPS-FGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIRDANIR-RFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILG----TQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRGQPDKAHPQKDV 671
            P+A VNV PSRHD   ETGRDPTRVK+FDTTLRDGEQSPGCTMTREEKL+VA+QLSKLGVD+IEAGFPIAS GDFEAV+EIA  VG  +NPPIICGLARAL KDID+CA+AV  ARFPRIHTFIATSDIHM+HKLKKTREQVL IT EMV+HA+S ++DVEFSAEDALRSD +FL++V+SVAI AGA+TINVPDTVGYTTP EF  L+H+LR  V GI+GVTISVHGH+DLGMAVANF++AIEGGARQ VECT+NGIGERAGNAALEEIVMALHVRK Y+N + F RP  SE  LTN+ TQE+ +SS+LVSSMTGM VQANKAIVGANAF+HESGIHQDGVLKNKETYEIMDA LVG+ Q+++LVLGKHSGRHAFRSRL E GY+L DDELN+AF RFKDLADKKKEIS LDL SIVN+E+R+A     F L G+ V AG  N PTATV ++D ++  E+ + AIGTGPVDA ++AI+  +     T + +L+EYSV SVTAGIDALGEVTVRV+D  + R + G+ ADTDVI+ASA AY+ AINR++++R  P   HPQKD 
Sbjct:   16 PVAAVNVRPSRHDTDTETGRDPTRVKIFDTTLRDGEQSPGCTMTREEKLVVARQLSKLGVDVIEAGFPIASPGDFEAVKEIAETVGTANNPPIICGLARALKKDIDVCAEAVRPARFPRIHTFIATSDIHMEHKLKKTREQVLAITHEMVSHAKSLIDDVEFSAEDALRSDPEFLAKVFSVAIAAGASTINVPDTVGYTTPGEFKSLIHYLREHVAGIDGVTISVHGHNDLGMAVANFLAAIEGGARQ-VECTVNGIGERAGNAALEEIVMALHVRKGYFNAAAFARPADSERALTNVVTQELYKSSRLVSSMTGMTVQANKAIVGANAFAHESGIHQDGVLKNKETYEIMDAALVGVVQESNLVLGKHSGRHAFRSRLAEMGYTLADDELNKAFARFKDLADKKKEISVLDLESIVNEEMREATGHIAFALRGVQVLAGNRNTPTATVIIHDKDADTESHIPAIGTGPVDATFQAINAAVAGHARTGDMKLLEYSVDSVTAGIDALGEVTVRVQDATTGRTFFGRGADTDVIYASAMAYVDAINRIVAARAGPTPKHPQKDT 577          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: A0A7S2WKU9_9STRA (2-isopropylmalate synthase n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WKU9_9STRA)

HSP 1 Score: 734 bits (1895), Expect = 1.830e-258
Identity = 387/554 (69.86%), Postives = 457/554 (82.49%), Query Frame = 0

Query:  121 VNVEPSRHDKETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYS-LTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIRDANIR-RFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILGTQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRGQ-PDKAHPQKDV 671
            V  +P+RHD ETGRDP RVK+FDTTLRDGEQSPGCTMT EEK+ VA+QL++LGVDIIEAGFP AS GDF+AV  IA  VG  ++PPIICGLARA+ KDI+ CA+A+  A+FPRIHTFIATSDIHM+HKLKK+R++VLDIT EMV++ARS  EDVEFSAEDALRSD +FL EVYS AIEAGATT+NVPDTVGYTTP+EF  L+  +R  VRGIE VTISVHGHDDLGMAVANFMSAIEGGARQ VECTINGIGERAGNAALEEIVMALHVRK+YYNP FGRP  SE PLTNI TQEI +SS+LVS+MTGM VQ NKAIVGANAF+HESGIHQDGVLKNK TYEIMDAELVGL ++  LVLGKHSGRHAFRSRL E GY  L+++E+ RAF+RFK+LADKKKEI++LD+ S+VNDE + ANI  RF+L  + V  G+ + PT             T  A+ GTGPVDAA++AI  I+  Q+  L+EYSV+SVTAGIDALGEVT RVRDE+  R Y G+++D DV+ ASA+A+++A+NR ++SR     + HPQ+D+
Sbjct:   70 VCTQPTRHDMETGRDPERVKIFDTTLRDGEQSPGCTMTAEEKIQVARQLARLGVDIIEAGFPFASAGDFDAVSRIAKEVGNLEDPPIICGLARAMKKDIETCARAIEHAKFPRIHTFIATSDIHMEHKLKKSRDEVLDITTEMVSYARSFCEDVEFSAEDALRSDPEFLYEVYSRAIEAGATTLNVPDTVGYTTPREFQTLIEGMRTHVRGIESVTISVHGHDDLGMAVANFMSAIEGGARQ-VECTINGIGERAGNAALEEIVMALHVRKSYYNPRFGRPAESETPLTNIMTQEIFKSSRLVSTMTGMAVQPNKAIVGANAFAHESGIHQDGVLKNKATYEIMDAELVGLAENL-LVLGKHSGRHAFRSRLVELGYEKLSEEEIQRAFVRFKELADKKKEITNLDIESLVNDETQ-ANIDDRFKLEQLQVFCGSPSTPTXXXXXXXXXXXSVTTRASTGTGPVDAAFQAIKQIIPAQDCTLLEYSVSSVTAGIDALGEVTCRVRDEVVDRVYYGRSSDNDVVVASAQAFINALNRAVASRDDHSQRFHPQRDI 620          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: A0A4D9D6G1_9STRA (2-isopropylmalate synthase n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D6G1_9STRA)

HSP 1 Score: 728 bits (1880), Expect = 5.940e-257
Identity = 384/550 (69.82%), Postives = 453/550 (82.36%), Query Frame = 0

Query:  121 VNVEPSRHDKETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGY-SLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIR-DANIRRFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILGTQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRGQPDKAHPQ 668
            V   P+RHD+ETGRDP RVK+FDTTLRDGEQSPGCTMT EEKL VAKQL+KLGVD+IEAGFPIAS GDF+AV+ IA  VG  +NPPIICGLARAL KDI+ C  AV  A FPRIHTFIATSDIHM HKLKK+RE+VL ITREMVT+ARS  EDVEFSAEDALRSD  FL +VYSVA+EAGATT+NVPDTVGYTTP EF  L+  LR  V+G+EGVTISVHGHDDLGMAVANFM+AIEGGARQ VECTINGIGERAGNAALEEIVMALHVRK++YNP FGR    E  LTNI+T EI ++S+LVS++TGM++Q NKAIVGANAF+HESGIHQDG+LKNK TYEIMDAE +GL ++  LVLGKHSGRHAFRSRL E GY +L+D ELN+AF+RFK++ADKKKEI++LDL SIVN+E+R + +  ++EL+   V  G +  PTAT+TL + E  +E  VAA GTGPVDAAY A+   LG +  +L+E+ V+SVTAGIDALGEVTVR+ D  S + Y G++A+TDV+ A+ +AYL A+NR+  SRG     HPQ
Sbjct:   22 VRPVPTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALRKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRAHVKGVEGVTISVHGHDDLGMAVANFMAAIEGGARQ-VECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKN-GLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLEYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVVAATQAYLGALNRVAISRGSLP-VHPQ 568          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: A0A6V1NWK8_HETAK (2-isopropylmalate synthase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1NWK8_HETAK)

HSP 1 Score: 727 bits (1876), Expect = 1.050e-255
Identity = 389/552 (70.47%), Postives = 452/552 (81.88%), Query Frame = 0

Query:  121 VNVEPSRHDKETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIRDANIRRFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILGTQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRGQ-PDKA-HPQKD 670
            + V+P+ HD  TGRDPTRVKVFDTTLRDGEQSPGCTMT EEK+ VAKQL++LGVD+IEAGFP AS GDF+AV+ IA  VGR +NPPIICGLARAL  DI+ CA+AV  A+FPRIHTFIATSDIHM+ KLKKTREQV+ ITREMV  ARS VEDVEFSAEDALRSD DFL EVYSVAIEAGATTINVPDTVGYTTP EF  L+H LR  VRGI+ VTISVHGHDDLGMAVANF++AIEGGARQ VECTINGIGERAGNAALEE+VMAL+VRKA+YNP+FGR    E  LTN+ T+EI ++S+LVSSMTGMMVQ NKAIVGANAF+HESGIHQDGVLKNK TYEIMDAE VGL  D  LVLGKHSGR AFR+RL E GY + ++EL RAF RFK LAD KKEI+++DL +IVNDEIR     RF+L  + V  G AN PTAT  + D+++G E    +IGTGPVDAA+K I + +  Q+ +L EY+V+SVTAGIDALGEVTVR+ D  S + + G+A++TDV+ ASA+AY++A NR+  SR   P K  HPQ+D
Sbjct:   61 ICVKPTAHDLATGRDPTRVKVFDTTLRDGEQSPGCTMTEEEKIAVAKQLARLGVDVIEAGFPFASPGDFKAVKRIAESVGRLENPPIICGLARALKADIEACAEAVKPAKFPRIHTFIATSDIHMEAKLKKTREQVVAITREMVGLARSLVEDVEFSAEDALRSDPDFLCEVYSVAIEAGATTINVPDTVGYTTPDEFYALMHKLRAGVRGIDQVTISVHGHDDLGMAVANFLAAIEGGARQ-VECTINGIGERAGNAALEEVVMALNVRKAHYNPAFGRATDDERALTNVNTREIYKTSRLVSSMTGMMVQPNKAIVGANAFAHESGIHQDGVLKNKRTYEIMDAETVGL-DDNLLVLGKHSGRAAFRARLTELGYEMAEEELMRAFSRFKVLADVKKEITNMDLEAIVNDEIRADVNDRFKLDTIQVLCGDANIPTATARILDTQTGSEIVTNSIGTGPVDAAFKCILEAVKVQDVKLQEYAVSSVTAGIDALGEVTVRLLDLTSGKVFFGRASNTDVVVASAQAYINACNRVQISRETLPVKPLHPQRD 610          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: A0A7S0VM37_9CRYP (2-isopropylmalate synthase n=2 Tax=Hemiselmis TaxID=77924 RepID=A0A7S0VM37_9CRYP)

HSP 1 Score: 721 bits (1861), Expect = 6.600e-253
Identity = 381/558 (68.28%), Postives = 448/558 (80.29%), Query Frame = 0

Query:  121 VNVEPSRHDKETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIRDANIRRFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILG--------TQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRG--QPDKAHPQ 668
            V VEP++HD  TGRDP R+KVFDTTLRDGEQSPGC+MT EEK+ VAKQL+KLGVD+IEAGFPIAS  DF AV  IATVVG  +NPPIICGLARA   DI+ CAKA+S A FPRIHTFIATSDIHM+HKLKKTRE+VL IT EMV++A+S V+DVEFSAEDALRSD+ FL+EVYSVA++AGATT+NVPDTVGYTTP EF  LV +LR  V GIEGVTISVHGH+DLGMAV+NF+SAIEGGARQ VE TINGIGERAGNAALEE+VM L+VRK YYN   GRP  SEA LTNI  +EI RSSKLV+++TGM VQ NKAIVGANAF+HESGIHQDG+LKNK TYEI+DA  +GL ++  +VLGKHSGR AFRSRL E G  L+DDELN+AF+RFK++ADKKKEI+++D+ SIV DE +D  + RF+L  + V  G     TATVTL DS+   E   AA GTGPVDAA++A++ + G         +  +LMEYSV SVTAGIDALGEVTVR+ D  S R + G+A++TDV+ AS  AY+HA+NRLL  R   QP K HPQ
Sbjct:   84 VCVEPTKHDLATGRDPARIKVFDTTLRDGEQSPGCSMTSEEKMQVAKQLAKLGVDVIEAGFPIASNDDFAAVNAIATVVGNQENPPIICGLARATKGDIEACAKAISPAAFPRIHTFIATSDIHMEHKLKKTREEVLAITAEMVSYAKSFVDDVEFSAEDALRSDWAFLAEVYSVAVKAGATTLNVPDTVGYTTPAEFKALVEYLREHVEGIEGVTISVHGHNDLGMAVSNFLSAIEGGARQ-VEVTINGIGERAGNAALEEVVMGLYVRKNYYNERLGRPADSEAELTNINKKEIHRSSKLVTALTGMPVQPNKAIVGANAFAHESGIHQDGMLKNKLTYEIVDASTIGLEKNDGIVLGKHSGRAAFRSRLTELGIELSDDELNKAFVRFKEVADKKKEITNMDIESIVGDEAQDTTLDRFKLKHVQVQCGDKAIATATVTLMDSQEDVEVTDAATGTGPVDAAFQAVNRLCGLSGAGQACAEQVKLMEYSVTSVTAGIDALGEVTVRLADVKSGRTFYGRASETDVVVASTAAYVHALNRLLVQRSDLQP-KLHPQ 639          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: A0A7S0EBN5_9CRYP (2-isopropylmalate synthase n=3 Tax=Geminigeraceae TaxID=589343 RepID=A0A7S0EBN5_9CRYP)

HSP 1 Score: 707 bits (1824), Expect = 3.080e-247
Identity = 372/568 (65.49%), Postives = 450/568 (79.23%), Query Frame = 0

Query:  113 GGMPPLARVNVEPSRHDKETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIRDANIRRFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILGTQ--------NTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRGQ-PDKAHPQKDV 671
            GG   +  VN++PS+HD  TGRDPTRVKVFDTTLRDGEQSPGC+MT +EK+ VAKQL+++GVD+IEAGFPIAS  DF AV++IA VVG  +NPPIICGLARA   DI  CA+AV  A+FPRIHTFIATSDIHM+HKL+K+RE+VL IT EMV  A+S V+DVEFSAEDALRSD  FL++V+SVAI+AGATTINVPDTVG+TTP EF   + +LR+   GIE VTISVHGHDDLGMAVANF+ AIEGGARQ VE T+NGIGERAGNAA+EE+VMALHVRK+YYNP FGRP  S   LTNI  +EI RSS+LV+S+TGMMVQ NKAIVGANAF+HESGIHQDG+LKNK TYEI+DA  +GL     +VLGKHSGR AF++RL + G  L +DE N+AF+RFKDLADKKKEI++ DL SIVNDE +D    RF+++ + V  G +  PTATVTL D +   E   A+ GTGPVDAA++A++ + G Q        + +LMEYSV+SVTAGIDALGEVTVR++D  S R ++G+AADTDV+ AS  AY++A+NRL+  R     K HPQ  V
Sbjct:   80 GGDATMKPVNMKPSKHDLATGRDPTRVKVFDTTLRDGEQSPGCSMTSDEKMQVAKQLARMGVDVIEAGFPIASNDDFIAVKQIADVVGNYENPPIICGLARATEGDIRRCAEAVKGAKFPRIHTFIATSDIHMEHKLRKSREEVLKITEEMVKLAKSLVDDVEFSAEDALRSDRAFLAQVFSVAIKAGATTINVPDTVGFTTPTEFKETIEYLRKHTVGIEDVTISVHGHDDLGMAVANFLGAIEGGARQ-VEVTVNGIGERAGNAAMEEVVMALHVRKSYYNPMFGRPADSNDALTNIVLKEIHRSSRLVTSLTGMMVQPNKAIVGANAFAHESGIHQDGMLKNKLTYEIIDASTIGLDNSDGIVLGKHSGRAAFKARLADLGIVLNEDETNKAFIRFKDLADKKKEITNADLESIVNDEAQDLAANRFKVMHVQVTCGISMVPTATVTLRDEKDNLELTDASTGTGPVDAAFQAVNRLCGIQGGGQANAADVKLMEYSVSSVTAGIDALGEVTVRLQDPRSGRVFLGRAADTDVVVASCVAYVNALNRLILQRSDLAPKLHPQLSV 646          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: M2XVI5_GALSU (2-isopropylmalate synthase n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2XVI5_GALSU)

HSP 1 Score: 682 bits (1761), Expect = 1.640e-237
Identity = 359/557 (64.45%), Postives = 429/557 (77.02%), Query Frame = 0

Query:  116 PPLARVNVEPSRHDKETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGYSLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIRDANIRRFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILGTQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHASAEAYLHAINRLLSSRGQPDKAHPQKDVL 672
            PPL     +P++HD  TGRDPTRVK FDTTLRDGEQSPG T+T EEKL++A+QL+KLGVDIIEAGFP+AS GDFEAV+ IA  VG  +NPPIICGLARA+  DI  C +AV  A F RIHTFIATSDIHM+HKLKK+R QVL+     V +A+S  ED+EFSAEDA RSD +FL EV+S AIEAGATTINVPDTVGYTTP EF  L+  +RR VRGIE V +SVHGHDDLG+AV+NF+SAIE GA QQVECT+NGIGERAGNA+LEEIVMAL+VR+ YYN   GRP    A LT + TQEI ++SKLVS++TGM+VQ NKAIVG+NAF+HESGIHQDG+LKN+ TYEIMDA L+G+ Q+  LVLGKHSGRHAFR RL E G+ LT+DELNRAF+RFK+LADKKKE++  DL SIVNDE+R       +L  + V  G     TATVT+   E  +E    +IGTGPVDAA+KA++ I G     L EY V S+T GIDALGEVTVR+R E   R +IG AA+TD++ AS +AY++A+NRL      P   HPQKD +
Sbjct:  109 PPLPPPIWKPTKHDLVTGRDPTRVKFFDTTLRDGEQSPGATLTGEEKLVIARQLAKLGVDIIEAGFPVASNGDFEAVRNIARTVGTRENPPIICGLARAVESDIARCWEAVKDAAFARIHTFIATSDIHMEHKLKKSRSQVLETVTRTVQYAKSLCEDIEFSAEDATRSDPEFLYEVFSRAIEAGATTINVPDTVGYTTPAEFGSLMRGIRRNVRGIENVCLSVHGHDDLGLAVSNFLSAIENGA-QQVECTVNGIGERAGNASLEEIVMALYVRRQYYNNKLGRPD-PNALLTCVDTQEIWKTSKLVSNLTGMIVQPNKAIVGSNAFAHESGIHQDGILKNRLTYEIMDARLIGM-QENQLVLGKHSGRHAFRKRLGELGFDLTEDELNRAFVRFKELADKKKEVTLWDLESIVNDEVRLQGTEYLKLERVQVQCGDKLISTATVTIMHMELSRELTAVSIGTGPVDAAFKALNSIEGVPKVVLQEYMVNSITQGIDALGEVTVRIRGETDSRSFIGSAANTDIVVASVQAYVNALNRLARYAQYPKSVHPQKDAV 662          

BLAST of mRNA_P-fluviatile_contig58.12223.1 vs. uniprot
Match: W7TP25_9STRA (2-isopropylmalate synthase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TP25_9STRA)

HSP 1 Score: 707 bits (1826), Expect = 2.550e-237
Identity = 373/527 (70.78%), Postives = 436/527 (82.73%), Query Frame = 0

Query:  121 VNVEPSRHDKETGRDPTRVKVFDTTLRDGEQSPGCTMTREEKLMVAKQLSKLGVDIIEAGFPIASQGDFEAVQEIATVVGRGDNPPIICGLARALVKDIDICAKAVSKARFPRIHTFIATSDIHMQHKLKKTREQVLDITREMVTHARSHVEDVEFSAEDALRSDFDFLSEVYSVAIEAGATTINVPDTVGYTTPKEFIGLVHHLRRTVRGIEGVTISVHGHDDLGMAVANFMSAIEGGARQQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFGRPVLSEAPLTNIKTQEIVRSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGY-SLTDDELNRAFLRFKDLADKKKEISSLDLASIVNDEIR---DANIRRFELVGMLVAAGTANKPTATVTLYDSESGKETCVAAIGTGPVDAAYKAIDDILGTQNTRLMEYSVASVTAGIDALGEVTVRVRDELSRREYIGKAADTDVIHA 643
            V   P+RHD+ETGRDP RVK+FDTTLRDGEQSPGCTMT EEKL VAKQL+KLGVD+IEAGFPIAS GDF+AV+ IA  VG  +NPPIICGLARAL KDI+ C  AV  A FPRIHTFIATSDIHM HKLKK+RE+VL ITREMVT+ARS  EDVEFSAEDALRSD  FL +VYSVA+EAGATT+NVPDTVGYTTP EF  L+  LR  V+G+EGVTISVHGHDDLGMAVANFM+AI GGARQ VECTINGIGERAGNAALEEIVMALHVRK++YNP FGR    E  LTNI+T EI ++S+LVS++TGM++Q NKAIVGANAF+HESGIHQDG+LKNK TYEIMDAE +GL ++  LVLGKHSGRHAFRSRL E GY +L+D ELN+AF+RFK++ADKKKEI++LDL SIVN+E+R   DA   ++EL+   V  G +  PTAT+TL + E  +E  VAA GTGPVDAAY A+   LG +  +L+E+ V+SVTAGIDALGEVTVR+ D  S + Y G++A+TDV+ A
Sbjct:  170 VRPVPTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQ-VECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKN-GLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAG--KYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSA 692