mRNA_P-fluviatile_contig1.477.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 1156 bits (2991), Expect = 0.000e+0
Identity = 609/972 (62.65%), Postives = 718/972 (73.87%), Query Frame = 1

Query:    1 TTAKGEFGGHQRAFEAAAAWTADR--NHQHHTRAPHLCCTDYEHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVSHAHATSISQNLRQYRLASSAPFPSVLKIAPGLLEHEEDYRGDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLLSGWFSVV-----AGGRRHDGEAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKALRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIA-----------IIQYINFIKSDDVPGSNGYSFTSSRGDQFPWLPADDFEYDDIAGHGTHTAGSAAGATLTNPAEPVTCEVGKDVSCVGGCIDGSAWFATDDLVTTSTATFLDIDRLCPAFG---CDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIVRDSCTISSPAIMKNALAVGATTSGSTRLTTT-GLVTPEVDATDIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSGCEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWASGVTDTVNNNERVVVDAGDVEGGVWSVWVWAKRLTTDTQSYSLVVNGAISPATDIGAIERSSSNLSISS 2850
            TTAK +F GH RAFEAAA W  +R  + Q  TRAPHLCC +Y HGRE +SRL+  LS EAVR V HSE  GAC+  + SHA A++I ++  Q+ L S +PFPS LK+APGL++H + +  D  T+  L R L ARHG+ MR  +VEGLS+EL PG L + S  A  F+ D L DL+  S+DLH+TNFWSD  +  GE+ +  GGAVR RDWSKAA+VV  LSE+A TSP DICSWN V VHHA  D+LL+SG   ++     AGGR   GE+AELH+AC+MGLVSFLA +PEV+RVAPR  +ALLNASARANIQSATVTDTPLTDAGL+G GQV+QVIDSG++ESSC+FA+D +G  V HG++F+E+G N PL SSS         S   +  D      R        ++ Q++E               IIQYIN IKSDD  GS+G SFT+S G Q+PWLPAD FEYD  AGHGTH AGS AGATL  PAE ++C+  + +SCVG C+D       DDL T +    +D+DRLCP F    CD    E+CLSDDV+ETLTNNGGMAQGAKLAIFD FY  FGL  F GNG+W  CAEA CK+HS+S+G D+ C LGP DVLYD +M  NPE+LLIFAAGN+G+  R +CT+SSPAI KN LAVGA+TSG TRL+ T GL         IDTVA FSS GPT+D RIKPEV+APGD IYSAAS+GS  + SCRLWAYAGTSM+ PIVAGA AM+RQYFM   F+ATD+TARGFCD+W+GCEAFSPSSATLKAL+INSANLMGG SEP+GSRGFGRIHL+AGMPL G GSLALFV+D+NYTSI E TR EYNFDV+  A LD R TLSW+DPPA T S VQLVHDLDL VISP+G R+TMWASGVTD+ N NERV+VDAGDVE G WSVW+WA  LTTD QSYSLVVNGAISP T +GAIERSSS  S SS
Sbjct:   33 TTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDR-LSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSF--------SSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVE-IVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGL--------GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSS 986          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1004 bits (2597), Expect = 0.000e+0
Identity = 545/945 (57.67%), Postives = 664/945 (70.26%), Query Frame = 1

Query:    1 TTAKGEFGGHQRAFEAAAAWTADR--NHQHHTRAPHLCCTDYEHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVSHAHATSISQNLRQYRLASSAPFPSVLKIAPGLLEHEEDYRGDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLLSGW----FSVVAGGRRHDGEAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKALRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIAIIQYINFIKSDDVPGSNGYSFTSSRGDQFPWLPADDFEYDDIAGHGTHTAGSAAGATLTNPAEPVTCEVGKDVSCVGGCIDGSAWFATDDLVTTSTATFL-DIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIVRDSCTISSPAIMKNALAVGATTSGSTRLTTTGLVTPEVDATDIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSGCEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWASGVTDTVNNNERVVVDAGDVEGGVWSVWVWAKRLTTDTQSYSLVVNGAISPATDIGA 2814
            TTAK +F GH RAFEAAA W  +R    Q  TRAPHLCC +Y HGRE +SRL+  LS +AVR V HSE HGAC+L T SHA A++I ++  Q+ L S +PFPS LK+APGL++H E    +   S    R LGARHG  MR  +VEGL+V+L P  LPA SAGA  F+ D+L DL+S S+DLH+TNFWSDP +  GE  +  GGAVR RDWSKAA+VV  LSE+A TSP DICSW+ V +  AGDD LL++G     FS    G  H+ E+ ELHVAC+MGLVSF AGRPEV+RV+PR+ + +LN +AR  IQSA+ TDTPLTDAGL+G G+VIQV+D+GL+E+SC+F +D  G  V HGYFF+E  F G   SS  +                           G  T+   +E    +IQYI  +     P S+  +F++++G  +P+LP D+F  DD AGHGTHTAGSAAGATL +PAE +TC   + + CVGGCID S    TDDLV+ +    L D+DRLCP +GCD S  + CLSD+V ETLT NGGMAQGAKL+IFD F+  FGL++  GNG+W  C EAGCK+HS S G D LC  G +++LYD FMYENPE+LLIFAAGN+G+  R  CT++SPAI KN LAVGA+TSG TR+ +T           ID VA FSS G T DGRIKPEV+APGDM+YSAAS+G++ + SCRLWAY GTSM+CPIVAGA AMVRQYF+   FY  D+TARGFC D   CE FSPS+AT+KALLINSANLMG  S+P+  RGFGRIHL+ GMPL G G LALFVADA  TSI+E T  EY FDVD  AGL+ RATLSWID PA + S +QLV+DLDL V+SPSG  + MW S VTD  N NERV+VDA DVE G W+V V++  L TD+QSYSLVVNGAIS  T  GA
Sbjct:   43 TTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDR-LGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDG-GEEVDHGYFFEEL-FVGSTGSSQLIYYPVFEA--------------------GDFTY---TEERRKVIQYIELVN----PDSDYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESE--TTDDLVSYAIQIALPDLDRLCPMYGCDGST-DPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVISTE------GGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTD-SHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAISAGTGEGA 947          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 988 bits (2553), Expect = 0.000e+0
Identity = 559/1009 (55.40%), Postives = 669/1009 (66.30%), Query Frame = 1

Query:    1 TTAKGEFGGHQRAFEAAAAWTADR--NHQHHTRAPHLCCTDYEHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVSHAHATSISQNLRQYRLASSAPFPSVLKIAPGLLEHEEDYRGDDATSKSLGR-RLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQD-GEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLLSGW----FSVVAGGRRHDGEAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKALRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIAIIQYINFIKSDDVPGSNGYSFTSSRGDQFPWLPADDFEYDDIAGHGTHTAGSAAGATLTNPAEPVTCEVGKDVSCVGGCIDGSAWFATDDLVTTSTATF-LDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIVRDSCTISSPAIMKNALAVGATTSGSTRLTTTGLVTPEVDATDIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSGCEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWASGVTDTVNNNERVVVDAGDVEGGVWSVWVWAKRLTTDTQSYSLVVNGAISPATDIGAIERSSSNLSISSASSSSSISPGDPGITPEEANGSSRSLTALMVSSFYVSLMFGFVASIVIAG 3000
            TTAK +F GH  AFEAAA W  +R  + Q  TRAPHL C  Y  G EA SRL+  LS EAVR V HSE HGAC+  + SHA A +I ++  Q+ L + +PFPS LK+APGL++H E +   +  ++S G  RL ARHG+ MR+ +VEGLSVEL PG L A S+ A  F+  ML DL+S S+DLH+TNFWSDP +   GE  +   GAVR RDW KAA+VV  LSE+AST P DICSWN + VHHAGDD LL+SG     FS    G  H+ E+ ELHVAC+MGLVSFLAGRPEV+RV+PR  + +LNA+AR  IQSA+ TDTPLTDAGL+G G++IQVIDSGL+E+SCYF +D+ G  V HGY+FDE G                                        R  +  S Y                  S+  + T   G  F + P         AGHGTHTAGSAAGATL +PAE +TC V   +SCVG CID     ATDDLV+ S+ T   D+DRLCP +GCD+S    CLSDDV ETL+NNGGMAQGAKLAIFD FYG +      GN +W  C EAGCKLHS+S G+D+ C +   D+L D FMYEN E+LLIFAAGNEG I R +CTI++P I KN+LA+GA+TSG TR+ +T      V AT IDTVA FSS G T D RIKPEV+APGD +YSAAS+G++ T SCRLWAY GTSM+CPIVAGA AM+RQYF+   FY  D+TARGFC D   CE FSPS+AT+KALLINSANLMGG SEP+G RGFGR+HL+AGMPL G G L LFVADA  TSI+  T  EY FDVD  AGLD RATLSWIDPPA   S VQLVH+LDL V+SP G  + MW S  TD  N NERV+VDA DV  G W+VWVWA  L TD+QSYSLVVNGAISP T  GA   SS  +S S+ S+SS    G  G+T         SL A   + F  +++   VA++V AG
Sbjct:   38 TTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDS-GEEVDHGYYFDELG----------------------------------------RAAVYSSSY------------------SSDETATIFNGGDFSYYPERR-----KAGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACID--LLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTRVIST------VGATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTD-THSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAISPGTGEGASGSSSFEVSSSATSASSEDGTGA-GVT-------GGSLVAAPAAGFLAAVVGACVAAMVAAG 965          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 868 bits (2244), Expect = 2.710e-299
Identity = 498/949 (52.48%), Postives = 605/949 (63.75%), Query Frame = 1

Query:    1 TTAKGEFGGHQRAFEAAAAWTAD---RNHQHHTRAPHLCCTDYEHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVSHAHATSISQNLRQYRLASSAPFPSVLKIAPGLLEHEEDYRGDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLLSGWFSVV---AGGRRHDGEAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKALRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIAIIQYINFIKSDDVPGSNGYSFTSSRGDQFPWLPADDFEYDDIAGHGTHTAGSAAGATLTNPAE-PVTCEVGKDVSCVGGCIDGSAWFATDDLVTT---STATFLDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIV--RDSCTISSPAIMKNALAVGATTSGSTRLTTTGLVTPEVDAT----DIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSG--CEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWA-SGVTDTVNNNERVVVDAGDVEGGVWSVWVWAKRLTTDTQSYSLVVNGAI 2790
            TTA   F GH + FE AA WT D      + + RAPHL CT+Y  G E  SRLQ LLS EAVR V HS  HGAC+ VT SHA A ++S N   Y L S +PFPS LK+APG+LEH       D  S+S GR L   HG  MR+ +VEGL++EL+PG LPAHS  A  F+ ++L+DL+S+S+DLH  N WSDP   +GE      GAVR+R+WS AA +V  L+ +  TSP DICSW+++ +HHAG+DLLL+SG   ++   +G    D EA EL VAC+MGLVSFL+ R EV RV+PR+   LLNA AR  IQSAT+T  PLT AGL+G GQVIQVI                                                                                    QYIN IK+D V G    S T+S G++  W+  D F+ DD+  HGTHTAGSA GAT+  PAE P TC   +   CVGGCID       DDL+T    +++   DIDR+CP  GCD+     CLSD V +TL ++GGMAQGAKL+ FD + G      F GNG+W  C EAGCK+HS S+G+D  C +  TD+LYD FMY NPE+L++ AAGN G+I   R  CT+ SPAI KNALAVGAT+SG TR+  T       D T    D+DTVA FSS GPT DGRIKPEV+APGD +YSA S+GS+   SCRL A +GTSMACPIVAGA AMVRQYFM  +FY  D  ARG+CDD SG  CE+FSPSSAT+KALLINSANLMGG SEP+G RGFGRIHL+ GMPL G GSLALFVAD++YTS+ EL+  ++ FDVD  A LDFRATLSWIDPPA++ SGVQLVHDLDL V+SPSG  H MWA SG  D+ N NERV++ A +VE G ++V V    L TD+Q+YSLVVNGA+
Sbjct:   43 TTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDN-PYYGLTSISPFPSALKVAPGVLEHGVSR---DQASQSPGR-LATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI------------------------------------------------------------------------------------QYINLIKADSVSGPA--SSTTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSD-VHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDD-SGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 855 bits (2209), Expect = 9.410e-297
Identity = 447/691 (64.69%), Postives = 515/691 (74.53%), Query Frame = 1

Query:  820 MGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKALRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIA-----------IIQYINFIKSDDVPGSNGYSFTSSRGDQFPWLPADDFEYDDIAGHGTHTAGSAAGATLTNPAEPVTCEVGKDVSCVGGCIDGSAWFATDDLVTTSTATFLDIDRLCPAFG---CDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIVRDSCTISSPAIMKNALAVGATTSGSTRLTTTGLVTPEVDATDIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSGCEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWASGVTDTVNNNERVVVDAGDVEGGVWSVWVWAKRLTTDTQSYSLVVNGAISPATDIGAIERSSSNLSISS 2850
            MGLVSFLA +PEV+RVAPR  +ALLNA+ARANIQSATVTDTPLTDAGL+G GQV+QVIDSG++ESSC+FA+D +G  V HG++F+E+G N  L SSS        +S                A     ++ Q++E               IIQYIN IKSDD  GS+G SFT+S G Q+PWL AD FEYDD AGHGTH AGS AGATL  PAE ++C   + +SCVG C+D       DDL T       D+DRLCP F    CD    ++CLSDDV+ETLTNNGGMAQGAKLAIFD FY  FGL  F G G+W  CA+A CK+HS+S+G D+ C LG  DVLYD +M  NPE+LLIFAAGN+G++ R +CTI SPAI KN LAVGA+TSG TRL+ T         + IDTVA FSS GPT+D RIKPEV+APGD IYSAAS+GS  T SCRLWAY GTSM+ PIVAGA AM+RQYFM   F+ATD+TARGFCD+W+GCEAFSPSSATLKAL+INSANLMG  SEPNGSRGFGRIHL+AGMPL G GSLALFV+D+NYTSI ELTR EYNFDV+  AGLD R TLSW+DPPA T S VQLVHDLDL VISP+G R+TMWASGVTD+ N NERV+VDA DVE G WSVW+WA  LTTD QSYSLVVNGAISP T +GAIERSSS  S SS
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSF-------SSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVE-IADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSAT-------PGSGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSS 676          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 852 bits (2200), Expect = 1.430e-293
Identity = 488/926 (52.70%), Postives = 593/926 (64.04%), Query Frame = 1

Query:    1 TTAKGEFGGHQRAFEAAAAWTAD---RNHQHHTRAPHLCCTDYEHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVSHAHATSISQNLRQYRLASSAPFPSVLKIAPGLLEHEEDYRGDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLLSGWFSVVA---GGRRHDGEAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKALRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIAIIQYINFIKSDDVPGSNGYSFTSSRGDQFPWLPADDFEYDDIAGHGTHTAGSAAGATLTNPAE-PVTCEVGKDVSCVGGCIDGSAWFATDDLVTTS---TATFLDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIV--RDSCTISSPAIMKNALAVGATTSGSTRLTTTGLVTPEVDAT----DIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSG--CEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWA-SGVTDTVNNNERVVVDAGDVEGGVWS 2721
            TTA+  F GH +AFE AA WT +      + + RAPHL CT+Y  G +   RLQ LLS  AVRPV HS  HGAC+ VT SHA A ++S +   Y L S +PFPS LK+APG+LEH       D  S+S GR L   HG  MR+ +VEGL++EL+PG LPAHS  A  F+ ++L+DL+S+S+DLH  N WSDP   +GE      GAVR+R+WS AA +V  L+ +  TSP DICSW+++ +H+AG+DLLL+SG   ++    G    D EA EL VAC+MGLVSFL+ R EV+RV+PR+   LLNA AR  IQSAT+T+ PLTDAGL+G GQVIQVID+GL+E+SC+F +D  G  V HG++++  G    +PSSSS                                                                                 DD+ GHGTH AGSA GAT+  PAE P TC   +   CVGGCID       DDL T     +   +DIDRLCP   CDD+    CLSDDV +TL ++GGMAQGAKLA FD F        F GNG+W  C EAGCK+HS S G+D+ C +   DVLYD FMY NPE+L++ AAGN GD    R  CT+ SPAI KNALAVGAT+SG TR+T T      +D T    D+DTVA FSS GPT DGRIKPEV+APGD IYSA S+GS+   SCRL A +GTSMACPIVAGA AMVRQYFM  +FY  D  ARG+ DD SG  CE+FSPSSAT+KALLINSANLMGG SEP+G RGFGRIHL+ GMPL G GSLALFVAD++YTS+ EL   ++ FDVD  A LDFRATLSWIDPPA++ SGVQLVHDLDL V+SPSG  H MWA SG  D+ N NERV++ A DVE G ++
Sbjct:   43 TTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDS-PYYGLTSISPFPSALKVAPGVLEHGVSL---DQASQSPGR-LTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDD-GEEVPHGHYYEALGEI--IPSSSSAR-------------------------------------------------------------------------------DDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSD-NHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDD-SGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 769 bits (1987), Expect = 9.470e-260
Identity = 452/958 (47.18%), Postives = 596/958 (62.21%), Query Frame = 1

Query:    1 TTAKGEFGGHQRAFEAAAAWTA-DRNHQHHTRAPHLCCTDYEHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVSHAHATSISQNLRQYRLASSAPFPSVLKIAPGLLEH---EEDYRGDDA---TSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAG---ATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLLSGWFSVVAGGRRHDG----EAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKALRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIAIIQYINFIKSDDVPGSNGYSFTSSRGDQFPWLPADDFEYDDIAGHGTHTAGSAAGATLTNPAEPVTCEVGKDV-SCVGGCIDGSAWFATDDLVTTSTATF-LDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIV-RDSCTISSPAIMKNALAVGATTSGSTRLTTTGL----VTPEVDATDIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSGCEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWASGVTDTVNNNERVVVDAGDVEG----GVWSVWVWAKRLTT-DTQSYSLVVNGAISP 2796
            TTA  +F   + AF  A+AW A DR  +    +P+L C ++  GR AY +L+G L A AV+PV  +  HGAC++VT S A A  +S         +   FP+ LKIAPGLL+H   +      DA   TS     RL   HG+ M+ D   GL VEL+PG LP+HS G   A  F++++L+ L S SLDLHA   WSD  ++ G+    A GA+R R+WS+AA +V   SES   +P+ +C W  VVVHHA +D+LL++G   ++  GR   G    EAAEL +ACYMGLVSFLA R EV++VA   ++  LNA+ARA IQSA+V  TPLTDAGL+G G+VIQ+ID+G+ E++C F     GT V+HGY+FD    + PL +SSS      +             R ++                   IQYI+ I  D   GS+            P++ AD F  DD  GHGTH A + AG+T+++PAE V+C  G +V  C GGCI       T+    +S A +  DI+RLCPAFGC     ++CL DD ++TL+ +GGMAQGAK+A  D FYG++   D AGNG+W TCA+AGCK+HS+S+G D  CQL   DVLYD +MYEN E LL+F+AGN G    RD+CTI SP I KN L VG+T+SG TRL+TT L         + + ID V+ FS  G T DGRIKPEV+APGDMIYSA+  G ++  SC L AYAGTS +  + +GA AMVRQYF  S+FY+ D+ +RGFCD    C++FSP + T+KA+LINSAN+MGG +EP+  RGFGRIHL+AGMPL G G +ALFVAD++   +    ++++ F+VD  AG++ RATLSW DP A+++SG QLV+DLDL V +P G R+ MW     DT N NERV+V A  V        W V V  +  T  DT S+SLVV GAISP
Sbjct:   36 TTAGADFDS-EAAFAFASAWQAGDRQGRS---SPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSS---WEDFTTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTC-FLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKV-------------------IQYIDLINPDTGDGSSS---------GMPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCI-------TESSTGSSEADYGEDINRLCPAFGCSG---DQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS--SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 945          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 722 bits (1864), Expect = 3.100e-245
Identity = 423/773 (54.72%), Postives = 506/773 (65.46%), Query Frame = 1

Query:  712 VHHAGDDLLLLSGWFSVVAGGRRHDGEAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKALRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIAIIQYINFIKSDDVPGSNGYSFTSSRGDQFPW-LPADDFEYDDIAGHG--------THTAGSAAGATLTNPAEPVTCEVGKDVSCVGGCIDGSAWFATDDLVTTSTATF-LDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIVRDSCTISSPAIMKNALAVGATTSGSTRLTTTGLVTPEVDATDIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSGCEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWASGVTDTVNNNERVVVDAGDVEGGVWSVWVWAKRLTTDTQSYSLVVNGAISPATDIGAIERSSSNLSISSASSSSSISPGDPGITPEEANGSSRSLTALMVSSFYVSLMFGFVASIVIAG 3000
            +H  G D LL SG       G  H+ E+ ELHVAC+MGLVS LAGRPEV+RV+PR  + +LNA+AR  +QSA+ TDTPLTDAGL+G G++IQVIDSGL+E+SCYF +D+ G  V HGY+FDE        S  S    A  T          P R +I                   IQYI+ +     P S   SF +S+G    + LP D F  DD A                SAAGATL NPAE +TC V   +SCVG CID      TDDLV+ S  T   D+DRLCP +GCD+S    CLSDDV ETL+NNGGMAQGAKLAIFD FYG++      GN +W  C EAGCKLHS+S G+D+ C +   D+L D FMYEN E LLIFAAGNEG   R +CTI++P I KN+LA+GA+TSG TR+ +T      V AT I+TVA FSS G T D RIKPEV+APGD +YSAAS+G++ T SCRLWAY GTSM+CPIVAGA AM+RQYFM   FY  D+TARGFC D   CE FSPS+AT+KALLINSANLMGG SEP+G RGFGR+HL+AGMPL G G+L LFVADA  TSI+E T  EY FDVD  AGLD RATLSWIDPP+   S VQLVH+LDL V+SP G  + MW S  TD  N NERV+VDA DV  G W+VWVWA  L TD+QSYSLVVNGAISP T  GA    SS+  +SS ++S+S   G        A  +  SL A   + F  +++   VA++V AG
Sbjct:    1 MHATGLDHLLFSGR----GAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDS-GEEVDHGYYFDELAQAAVYSSLYSSDETA--TIFNGGDFSYYPERRKI-------------------IQYIDLVN----PDSPYSSFYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACID--LLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEERSTCTINTPGIGKNSLAIGASTSGDTRVIST------VGATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTD-THSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAISPGTGEGA--SGSSSFEVSSLATSASSEDGT------AAGATGGSLVAAPAAVFSTAVLGASVAAMVAAG 726          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 711 bits (1836), Expect = 2.470e-235
Identity = 417/866 (48.15%), Postives = 546/866 (63.05%), Query Frame = 1

Query:  427 VEGLSVELAPGILPAH----SAGATFFV------EDMLEDLVSESLDLHATNFWS--------DPAVQDGEEFARAGGA--VRRRDWSKAAAVVQGLSES-ASTSPADICSWNTVVVHHAGDDLLLLSGWFSVVAGGRRHDG----EAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKA------LRTSRVVA----QVDLVPRRMRINASMGPRTFIQLSEYLIAIIQYINFIKSDDVPGSN-----GYSFTSSRGDQFPWLPADDFEYDDIAGHGTHTAGSAAGATLTNPAEPVT------CEVGKDVSCVGGCIDGSAWFATDDLVTTSTATF-------LDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGL-VDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEGDIVRDSCTISSPAIMKNALAVGATTSGSTRLTTTGLVTPEVDAT----DIDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSGCEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWASGVTDTVNNNERVVVDAGDV---------------EGGVWSVWVWAKRLT-TDTQSYSLVVNGAISPAT 2802
            V GL+VELAPG+L A     S+G  ++       ++  E+L+S+SLD+H  +FWS        D A  +G   +  GG   V  R+W++AA V++GL  +    +  D+CSW+ +VV H  DDLL + G   ++  GR   G    EA EL VAC+M L+SFLA RPEV+RV+P     LLNA+A  N+QSA++T++P+TDAGL+G G+VIQ++D+GL+ +SC+F ++  G  V HGY+F+E G       +SS G         ++ +RV        DL  R+  +  +  P           A+     +   +    +N     G S+ +S G+Q+PW+ A +F  DD  GHGTHTAGSAAGA +++P+   T      CE G+++SCVGGCID    F  DDL+TT    +       +D+DRLCP   CDD   E CL DD +ETL  +GG+A+GA+LAIFD      G+ +D AGNGMW    EAG KL S+SWG    C     DVL+D FMY N + LL+FAAGN+GD     CT+ +PA+ KN LAVGA+TSG  RL+ T       D      DID +A+FSS GPT D RIKPEV+APGDMIYSAA  G +   +CRLW YAGTSM+ PIVAGA A+VRQYF    FYA D+  RGFCD + GCE F+PS+AT+KA+LINSANLMGG +EP+G RGFGR+HL+AGMPLAG G  ALFVAD+  TS +ELT  EY+F VD AAG+D RATLSWIDPPA T S VQLVHDLDL+V SP+G+R++MW  G  D++NNNERV+V A ++                 G W+V VW+KRL   +TQSYSLVV GAI P T
Sbjct:  224 VTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVE-----ASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDW-SGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSG-DGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCDSF-GCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPPT 1081          

BLAST of mRNA_P-fluviatile_contig1.477.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 670 bits (1728), Expect = 7.750e-221
Identity = 429/1009 (42.52%), Postives = 576/1009 (57.09%), Query Frame = 1

Query:   16 EFGGHQRAFEAAAAWTADR---NHQHHTRAPHLCCTDYEHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVSHAHATSISQNLRQYRLASSAPFPSVLKIAPGLLEHEEDYR-----GDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPA--HSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLLSGWFSVVAGGRRHDGEAAELHVACYMGLVSFLAGRPEVVRVAPRYKRALLNASARANIQSATVTDTPLTDAGLNGRGQVIQVIDSGLEESSCYFANDAAGTRVKHGYFFDEFGFNGPLPSSSSVGGKA----LRTSRVVAQVDLVPRRMRINASMGPRTFIQLSEYLIAIIQYIN--FIKSDDVPGSNGYSFTSSRGDQFPWLPADDFEY--------------------------DDIAGHGTHTAGSAAGA-TLTNPAEPVTCEVGKDVSCVGGCIDGSAWFATDDLVTTSTATFLDIDRLCPAFGCD---DSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFY-GEFGLVDFAGNGMWATCAEAGCKLHSSSWGSDYLCQLGPTDVLYDTFMYENPEDLLIFAAGNEG---DIV-RDSCTISSPAIMKNALAVGATTSGSTRLTTTG----LVTPEVDATD--------------------------IDTVAFFSSTGPTADGRIKPEVMAPGDMIYSAASEGSEATDSCRLWAYAGTSMACPIVAGALAMVRQYFMQSAFYATDLTARGFCDDWSG---CEAFSPSSATLKALLINSANLMGGRSEPNGSRGFGRIHLDAGMPLAGNGSLALFVADANYTSISELTRVEYNFDVDGAAGLDFRATLSWIDPPANTMSGVQLVHDLDLAVISPSGVRHTMWASGVTDTVNNNERVVVDAGDVEGGVWSVWVWAKRLTTDTQSYSLVVNGAI 2790
            +F   Q A+ AA++W  DR   +    + AP++ C DY+ GR A + L+   +  AV  V ++E  G C++VT   + AT++ +    + L S+AP    LK+A  LL+H  D        DD         L + +G  M  D+V GLSV L+PG+L A    + A  F+ D   DL SES+++   +FWSD      E         R R+WS+AAAVV GL+  ++ +  +IC +  + + H  DDL+L+ G   ++ G R      +E   ACYMGL+S LA +PEV+RV+P     L NA A A +QSAT T+TPL DAGL+G G+VIQV+DSGL+E+SC+FA+D  G +V+HG+ FD          S+ +G  +    +   ++V  + L     + + S G  +F  +S+YL     Y +  +I  DD  G   YS T S  D F     DD  Y                          D + GHGT TAGSAAGA +  +  E   C   +   C GGCI  S     D ++  ST    D+D  CP + CD   D    ECLSDD  E L  +GG+A GA++++FD  Y G      FAGN +W +  E G K+H++SWG   LCQL   + LYDTFMYENPE LLIFAAGN+G   DI  R++CT+ SPA+ KN+LAVGAT+SG    T TG    LV  E+  TD                          +DTVAFFSS GPT DGRIKP+V+APGD + SA+S+G++   SC+L A  GTS +CP+VAGA A+VRQYF   +F+A D+ +RG C + S    C  F+PS+AT+KA++INSA+LMGG SEPNG RGFGR+HL+AGMP+ G G   L V +A      E+T +     VDG AG++ RATL+W+DPP   +S  QL HDLDL VI+PSG ++TMW SGV DTVN  ERV+V    +E GVWSV V AK L TD Q YSLVV GAI
Sbjct:   28 DFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDV-----TRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKR----FPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDD-GLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEY-YSDTWSE-DSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASD---VDAMLDDST---FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDG-HSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIAD-GEITEL---VTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1013