mRNA_P-lacustris_contig1017.246.1 (mRNA) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: D8LUB7_ECTSI (Intraflagellar transport protein 172 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LUB7_ECTSI)

HSP 1 Score: 1359 bits (3517), Expect = 0.000e+0
Identity = 761/1043 (72.96%), Postives = 811/1043 (77.76%), Query Frame = 2

Query:  818 EALSLGRVHGLPRSA*RKWPRNTSGFSWTHQARSGPPLSRRKRETTSKPSACTLRGGMPAQAGRVLKERSVENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVRRAVRPGCGRGRGRGSNCGGGP--VRACR-----QA*ESITVL*RGRHVEGCASCMPETFAPPRAQ--SPRAVPGCSGLDRQRWCQG-GLPQRWPRSGAKPGLGCRYRCLPQGYPECDDERRRPGGGVGEGHHGGQGRTAKQAHGGSARGVRPPGRHREARGGGGGAEGGRPAXXXXXXXXXXXRVG---KGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDEASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDREASARPGAQETVERLLRILQSQTQNLKASNKAAPAGFEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEKEASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQ 3907
            EAL+LGR H LP        ++        +        + K     +     L+GG+PAQAGRVLKERSVENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETL+G+SARPHLRTLARHHE+AGN+ LAERF+VDA+APQLAVEMYTKAN W+AAHKLA SYMSEGEVRVLYM QA+KME  GSLLEAEKLYLQVGE+DLA+AMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLK+AEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKF+GGQSA+KRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQ CCPAKLPGIHLKHALFLEDEERFKEAE EFLQAGKPREAIDMFVHQKAWADACRVAEGHDP  V   +          G         VRA +     Q  E   +      V  C   +P       AQ  + +A+ G  G        G  L Q    S A        +       + ++   R    +         R  +     S R +   GRH  A      AE  R A             G   K     +GH QL+EKVE AYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWE+AAKQ SG ETLAKYAGLRVRSVLDDEASWEK VSG  +RRELDDAVLVLQ+KGAPPITSG GRSS AVG GV SGSGGPAADMYERLVKA+LGRD+E SARP A ETVERLLR+LQ Q QNLK SNK +PAGFEHLLMATHYTCLMGRCREKGGKDCLELA+K+SITLLRYSD IP+DKCFYQA    GSLCKDLGNENLAFVLLNRYVDLTEAIDEG ASLLDNSDFAEATNVPLVD+ TLP KQ+IPVESEREE RDWVLSVCMDAKIDQALPPEKEA GTLY+       GLYASELPSCVVTG PVHKRDLIQ
Sbjct:  700 EALTLGRAHRLPEERLEGMAQDYFRLLVDTKQEERAAALKEKEGDYEQAIRLYLKGGLPAQAGRVLKERSVENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLDGDSARPHLRTLARHHEQAGNHALAERFYVDADAPQLAVEMYTKANRWEAAHKLASSYMSEGEVRVLYMEQAQKMEAVGSLLEAEKLYLQVGEMDLAIAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKEAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFYGGQSAHKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQACCPAKLPGIHLKHALFLEDEERFKEAEMEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPPAVSDVLCAQATDVAAAGDRAAAEDLYVRAAKPEKALQCYEEAGMWRDALRV--CQRHLPHLAHKVHAQYQAAQAMTGTGGAKADYLSAGRALEQNRDWSAAIDAYLKATQSATMNAEDLEEVWERA---ITVARVDLPNRHMEVVREVSRR-LADMGRHEAA------AEVLRAADQPEEAVAVAVAGGAWEKARESARGHGQLAEKVESAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWESAAKQESGVETLAKYAGLRVRSVLDDEASWEKPVSGSDDRRELDDAVLVLQEKGAPPITSGPGRSSFAVGAGVSSGSGGPAADMYERLVKAVLGRDKEPSARPAAHETVERLLRVLQDQAQNLKTSNKTSPAGFEHLLMATHYTCLMGRCREKGGKDCLELASKMSITLLRYSDFIPSDKCFYQA----GSLCKDLGNENLAFVLLNRYVDLTEAIDEGNASLLDNSDFAEATNVPLVDDRTLPTKQHIPVESEREEVRDWVLSVCMDAKIDQALPPEKEAGGTLYE-------GLYASELPSCVVTGLPVHKRDLIQ 1719          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: A0A835Z5T0_9STRA (Intraflagellar transport protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z5T0_9STRA)

HSP 1 Score: 853 bits (2203), Expect = 2.620e-279
Identity = 510/1070 (47.66%), Postives = 668/1070 (62.43%), Query Frame = 2

Query:  977 LRGGMPAQAGRVLKE--RSVEN-QGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGG-DAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVRRAVRPGCGRGRGRGSNCGGGPVRACRQA*ESITVL*R------------GRHVEGCASC---MPETFAPP--RAQSPRAVPGCSGL------------DRQRWCQGGLPQRWPRSGAKPGLGCRYRCLPQGYPECDDERRRPGGGVGEGHHGGQGRTAKQAHGGSARGVRPP----GRHREARGGGGGAEGGRPAXXXXXXXXXXXRVG---KGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDD-EASWEKQVSGGGNRRE--------LDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILG--RDREASARP---GAQETVERLLRILQSQTQ-------NLKASNKAAPAGFEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEKEASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQVNHARANKRDWNAYVRIFRQCPWTGLDATPQY 4003
            L+ G+PAQA RVLKE  RSV++ + Q LET+A+ L +AGMHD+AG+ +EE+ QLQRA+D+Y RG AFR+ V+LARRSFP EVV L+E WGD+L+ QKQ+DMAINHYIE RAN KA+ A L++RQW+KAAQL+E L+G +ARP+ + LARHHE+AG   LAE+F+V A+AP+LAV+MYTKAN W+AAHKLA SYMSEGEVR+LY+ QA+K+E  G L EAE+L +QV E D+A+++YK+ +R+D MVRLV  HR ++LKETH +LAQQLE+EG    AE HYA AGEWL+AVNM+R  DMW DALRVA+ HGG +A KRVAYAWALALGG D GA+ L K GL++ AI+YA E   F +A ELA+   PA+LP IHLKH +FLEDEERF EAE EF+ AGKPREA+DM VHQK W  A RVA+  +P  V   +           +  GG  + + R   E++ +               G++ E    C   +P   A    R Q+ +AV G  G               + W          R+G     G   + L   +    D  R                 A   H   AR V       GRH  A      A+  R A             G   K   +  GHA L+E+VE+AYQ+HL+RGEA +EL+++GQT+AALDI+AQ+G+W RLWE AA++      LA+YA LRV  +L + EA+ ++  +               + +AV  L ++G P     +G                    MYE LVKA+LG  ++ EA+A      ++E V  L  +L    Q         + S+KAA    EHLL+A HY+ L+ +C   GG+DC  LAA++++TLLRYSDL PADKCFY    RAG L +++G ++L FVLLNRYVDL EAIDEG ASL+DNSDFA ATNVP+VD +TLP   Y+  E++REE RDWVL VC+DA ++Q LP   +A GT+Y+        L+ASELP+CVVTGFP+ K+ L+ VN  +A+KRDWN YVR F+ CPWTG   +PQY
Sbjct:  665 LKAGLPAQAARVLKENERSVKSGRAQWLETLAAALGSAGMHDRAGDCFEELGQLQRALDAYVRGGAFRKGVDLARRSFPGEVVKLEEAWGDWLVGQKQLDMAINHYIEARANGKAMEAALSARQWTKAAQLVEALDGGAARPYYKKLARHHEDAGQLQLAEKFYVRADAPELAVDMYTKANQWEAAHKLASSYMSEGEVRMLYIDQAQKLEAIGKLREAERLLVQVNEADMAISLYKRHRRYDDMVRLVTAHRGELLKETHMFLAQQLEVEGDFTQAEGHYAAAGEWLAAVNMFRGLDMWEDALRVARHHGGAAAQKRVAYAWALALGGGDKGARALTKHGLVDAAIDYAAELRDFANAFELARAAAPARLPDIHLKHGMFLEDEERFAEAEAEFVLAGKPREAVDMLVHQKDWTGALRVAQEQEPAAVPDVLCAEADDALATAAAAGGEALESARGRAETLFLQASRPERALAMWEEAGQYTEALRVCGRHLPHRLAEVEGRNQAAQAVSGRGGTKANYLATARAYEQSRNWAGAIDALLQARAGTMATSGAGTQELEDVWSRALDIART---------------QAPNRHVAVAREVASRLVALGRHEAA------ADALRDARQLEEAAEVAMGGGAWDKAREVATGHAALAEQVERAYQRHLVRGEAADELVEIGQTSAALDIIAQRGDWQRLWEVAARENVPPAALARYAALRVTGLLKEVEAARQRATAAEDGXXXXXXXXXXXMAEAVGTLAERGLPGAKEAMG--------------------MYEGLVKAVLGMTQEEEAAAEKLPENSREIVAGLRGVLYDLWQATSGGKGGQQRSSKAAD-DVEHLLLAAHYSALLQQCVRHGGRDCAALAARMALTLLRYSDLAPADKCFY----RAGRLAREVGEQSLGFVLLNRYVDLAEAIDEGDASLVDNSDFAHATNVPVVDATTLPRYHYLGDEAKREEVRDWVLGVCVDACVEQQLPSAADARGTIYEV-------LFASELPTCVVTGFPIAKQ-LLDVNGTKASKRDWNTYVRTFKHCPWTGKPQSPQY 1680          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: A0A6H5KQ80_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KQ80_9PHAE)

HSP 1 Score: 815 bits (2106), Expect = 5.720e-278
Identity = 497/792 (62.75%), Postives = 527/792 (66.54%), Query Frame = 2

Query: 1652 VDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVR-----RAVRPGCGRGRGRGSNCGGGPVRACR-----QA*ESITVL*RGRHVEGCASCMPETFAPPRAQ--SPRAVPGCSG-----------LDRQR------------WCQGGLPQR-WPRSGAKPGLGCRYRCLPQGYPECDDERRRPGGGVGEGHHGGQGRTAKQAHGGSARGVRPPGRHREARGGGGGAEGGRPAXXXXXXXXXXXRVG---KGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDEASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDREASARPGAQETVERLLRILQSQTQNLKASNKAAPAGFEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEKEASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQV 3910
            +DLA+AMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLK+AEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKF+GGQSA+KRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQ CCPAKLPGIHLKHALFLEDEERFKEAE EFLQAGKPREAIDMFVHQKAWADACRVAEGHDP  V      +AV       R    +     VRA +     Q  E   +      V  C   +P       AQ  + +A+ G  G           L++ R            W  GG+    W  S   P          +   E  +             H    R   +        +   GRH  A      AE  R A             G   K     +GH QL+EKVE AYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWE+AAKQGSG ETLAKYAGLRVRSVLDDEASWEK V G  +RRELDDAVLVLQ+KGAPPITSG GRSSSAVG GV SGSGGPAADMYERLVKA+LGRD+EASARPGAQETVERLLR+LQ Q  NLKA                                                                GSLCKDLGNENLAFVLLNRYVDLTEAIDEG ASLLDNSDF EATNVPLVD+ TLP KQ+IPVESEREE RDWVLSVCMDAKIDQALPPEKEA GTLYQ       GLYASELPSCVVTG PVHKRDLIQV
Sbjct:    1 MDLAIAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKEAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFYGGQSAHKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQACCPAKLPGIHLKHALFLEDEERFKEAEMEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPPAVSDVLCAQAVDVAAAGDRAAAEDL---YVRAAKPEKALQCYEEAGMWRDALRV--CQRHLPHLAHKVHAQYQAAQAMTGTGGTKADYLSAGRALEQNRDWSAAIDAYLKVWLAGGIDVLDWRASAWMPQATQSATMNAEDLEEVWERAITVARVDLPNRHMVVVREVSRR-------LADMGRHEAA------AEVLRAADQPEEAVAVAVAGGAWEKARESARGHGQLAEKVESAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWESAAKQGSGVETLAKYAGLRVRSVLDDEASWEKPVPGSDDRRELDDAVLVLQEKGAPPITSGPGRSSSAVGAGVSSGSGGPAADMYERLVKAVLGRDKEASARPGAQETVERLLRVLQDQAHNLKA----------------------------------------------------------------GSLCKDLGNENLAFVLLNRYVDLTEAIDEGNASLLDNSDFTEATNVPLVDDRTLPTKQHIPVESEREEVRDWVLSVCMDAKIDQALPPEKEAGGTLYQ-------GLYASELPSCVVTGLPVHKRDLIQV 703          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: F0Y092_AURAN (Uncharacterized protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y092_AURAN)

HSP 1 Score: 799 bits (2063), Expect = 1.140e-257
Identity = 483/1056 (45.74%), Postives = 638/1056 (60.42%), Query Frame = 2

Query:  977 LRGGMPAQAGRVLKERSVENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVRRAVRPGCGRGRGRGSNCGGGPVRACRQA*ESITVL*R------------GRHVEGCASC---MPETFAP-----PRAQSPRAVPGCSGLD----RQRWCQGGLPQRWPRS-----GAKPGLGCRYRCLPQGYPECDDERRRPGGGVGEGHHGGQGR----TAKQAHGGSARGVRPPGRHREARGGGGGAEGGRPAXXXXXXXXXXXRV---------GKGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDEASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDREASARPGAQETVERLLRILQSQTQNLKASNKAA-----PAGFEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEKEASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQVNHARANKRDWNAYVRIFRQCPWTGLDATPQY 4003
            LRGGMP +A R++K+  + N   +LE V+++L+A G+H++AGEFYE M+QLQRAMD+Y +G +FR+AVELAR+ FP  VV+LQE+WGDYL    QV+MAINHYIE   ++KA+ A L++RQW+KAAQ+LE ++ + A+P+LR LARH+E++ +   AERF+V A AP  AVEMYT+AN WD AHK+A SYM   EV +LY+  A+K+E  G L +AEKLYL V E DLA+ MYKK +++DAMV+LV KHR ++LKETHQYLAQ LE EGSL+DAEHHY EAGEWLSAVNMYR++DMW +A+RVAK HGG +A KRVAYAWAL+LGGDAGAKLL KQGLIEPAI+YA ESGAFDHA ELA+  CP KLP +HLKHAL+LEDEER+KEAE EF+QA KPREAIDM++HQ+AWA+A  VA  +DP     +  P       R     G    A     E + +L              G   E  A     +P   A       +A++ R   G   +D     Q+W Q    ++W R+      A+PGL    + L + +    D  RR         H    +      K  H      ++  GRH            G  A            V          K   +  G + L  +V+ AYQ  L   E T+ LL++G   AALD+L ++ EWDRLW+ A ++       A+YAGL+   +L               + +L  AV  L+  GAPP                     GP   MY  LV A+LG+   A A    + +V  L  +L     +   + + A       GFE LLMATHY  L   C  +G KD   +A K++ITL+RYS +IP DK FYQA    G++ +D G++NLAFVLLNRY+DLTEAI+EG    +DN+DF +ATNVP   +  LP +QY+P E +REE RDWVL++CMD  +DQ LP +++A GT+Y       +GLYAS+LP+C+VTG+PV K +L+ VN + ANK DWN YVR  ++CPWT  +  P Y
Sbjct:  807 LRGGMPGRAARLIKQHGINNPPSILERVSASLTAGGLHEQAGEFYERMDQLQRAMDAYLKGASFRKAVELARKHFPGRVVELQELWGDYLFEHNQVEMAINHYIEASMSSKAIDAALSARQWTKAAQMLENVDLDIAQPYLRRLARHYEDSNDTSEAERFYVAAGAPDKAVEMYTRANLWDRAHKIASSYMEPREVSLLYISHAQKLEAEGKLKDAEKLYLTVDEPDLAINMYKKQRKYDAMVQLVEKHRRELLKETHQYLAQHLESEGSLRDAEHHYCEAGEWLSAVNMYRTNDMWEEAMRVAKLHGGPNASKRVAYAWALSLGGDAGAKLLTKQGLIEPAIDYAIESGAFDHAFELARSACPGKLPDVHLKHALYLEDEERYKEAESEFIQANKPREAIDMYIHQQAWAEALAVANKYDP-----SASPDVYVSHARAEADAGQHQHA-----EELFMLAAKPELALSMYRDAGMWTEALALAQRHLPHQLAEVSLAYSQAEAQRGTGGTK-VDFLSAGQQWEQ---QKQWDRAVEAYLNARPGLLEDPKELEEIWECAIDVARR---------HMPPEKFRDIAIKVTHK-----LKAIGRH------------GAAAEFLRELNDIDGAVRCAMDGRCWAKARELAIGSSTLEAEVDAAYQSALRSAEDTDGLLELGHRTAALDVLVERKEWDRLWQMADREQIHLSVRARYAGLQAAQIL-------------AAKGDLTQAVRTLKQHGAPPP--------------------GPNVQMYHDLVLAVLGQS-YAQANLDHEHSVSDLRDVLFHLASSHDGAREDALGTQGAGGFEQLLMATHYYRLYLTCVSQGLKD---IALKIAITLMRYSGIIPIDKAFYQA----GTMARDQGHDNLAFVLLNRYIDLTEAIEEGNIDSIDNADFVDATNVPFPFD--LPTQQYLPREDDREEIRDWVLTICMDKSVDQQLPAKQQALGTVY-------SGLYASDLPTCIVTGYPVQKWELLNVNKSVANKGDWNQYVRKVKKCPWTNKEQGPLY 1772          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: A0A7S2W0W8_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2W0W8_9STRA)

HSP 1 Score: 786 bits (2030), Expect = 7.880e-253
Identity = 483/1068 (45.22%), Postives = 639/1068 (59.83%), Query Frame = 2

Query:  977 LRGGMPAQAGRVLK------ERSVENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVRRAVRPGCGRGRGRGSNCGGGPVRACRQA*ESITVL*RGRHVEGCASCMPETFAPPRA-------QSPRAVPGCSGLDRQRWCQGGL---PQRWPRS-----GAKPGL---------------GCRYRCLPQGYPECDDERRRPGGGVGEGHHGGQGRTAKQAHGGSARGVRPPGRHREARGGGGGAEGGRPAXXXXXXXXXXXRVGKGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDE--------------------ASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDREASARPGAQETV--ERLLRILQSQTQNLKA-SNKAAPAGFEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEKEASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQVNHARANKRDWNAYVRIFRQCPWTGLDATPQY 4003
            L+GG+PA+A +++K      +RS  N  Q+LE VA+ L+ AG++D+AGEF+EEM+QLQ+A+DSY +G+A+RQAV+L+RR FP++V DL+E WGD+L+  KQVDMAINH+IE +   KA+ A L SRQW KAAQ  E LE E+ARP+ + +A+H+E+A  +  AE+++V A+A + AVEMYTK + WD AHKLA SYMS+ EV +LY+ QA++ME AG L EAE+L+L+V E DLA+ MYKK +++DAMVRLVAK+R ++LKETHQ+LAQ LE E +LKDAEHHY EAGEWLSAVNMYRS+DMW DA+RVAK HGG SA KRVA+AWALALGG+AGAKLL K GLIEPAI+YA E+G FDHA ELA+     KLP IHLKHALFLEDEE++ +AE+EF+ A KPREAIDM+VH + WA+A RVAE +DP  V             R        +       E    + +  ++   A  + +   P +        QS +A  G  G       QG +    ++W R+      A+PGL                 R  C  + Y E   E       +G   HG    T ++A                          G  A           R      + QG   L +KVE+AYQ HL+    T+ LL++G TNAALD+LA+  EWDRLW+ AAK+  G   LAKYA LR   +LD++                    AS     + G +R  LDDAV  L   GA   T+  G  +S                M  RL KA+L R R  + +   + TV  + L  +L+   Q     S++      + LL+ATHY+ L+   R  G  D  ELA K++I+LL ++D+IPADK FY A    G  CKD G+ NLAFVLLNRYVDL EAI+ G  +++DNSD  EATNVP  ++  LP KQ++  E EREE R+WVL+VCMD  +D ALP   EA GT+Y+       G+++SE   C+VTGFPV+  D + +N   ANKRDWN +V   +QCPWTG    PQ+
Sbjct:  771 LKGGVPARAAKLIKTKRLLADRSASNM-QMLERVANALATAGLYDRAGEFHEEMDQLQKALDSYIKGHAYRQAVDLSRRHFPSQVTDLEEAWGDWLVANKQVDMAINHFIEAQCATKAIEAALKSRQWGKAAQFAENLEPEAARPYFKRIAKHYEDARQFDEAEKYYVAAQATKTAVEMYTKNSMWDRAHKLATSYMSDREVGMLYISQAQRMEAAGKLREAEQLFLKVNEADLAINMYKKQRKYDAMVRLVAKYRKELLKETHQFLAQHLESEANLKDAEHHYCEAGEWLSAVNMYRSNDMWEDAIRVAKLHGGMSASKRVAFAWALALGGEAGAKLLTKLGLIEPAIDYAIETGGFDHAFELARSSLQRKLPEIHLKHALFLEDEEKYADAEDEFINANKPREAIDMYVHTQDWANALRVAETYDPAAVADVCVAQARAAAERRDFARAQELYLSASKPEFALTMFQEANMWQEALELAQKHLPHKLAEVNMAYQSAQASQGQGGSKADFLSQGRVWEEQRKWTRAIDAYLNARPGLLPPDELEQVWEAAVRVARQECRNR-YAEVVREVTSRLAEIGR--HGAAAETLREAQ----------------------DLDGAVAVALQGQCWDQARE-----LAQGQPALEDKVERAYQSHLVSANNTDGLLELGHTNAALDVLARGKEWDRLWDMAAKEHVGPTVLAKYAALRANQLLDEDDADSHNPHPRASXXXXXXXXASVTGWTTAGTSR--LDDAVAALHKYGASTSTAPAGLPTS----------------MLSRLTKALLSRPRSLAEKLEDRHTVSLQCLRDVLRLAAQEAADNSDRLRAKELQPLLLATHYSHLLQTSR--GEPDLKELAPKIAISLLAFNDVIPADKLFYDA----GIACKDQGHANLAFVLLNRYVDLIEAIEVGDPTIVDNSDLQEATNVPYAES--LPSKQHLTTEEEREEVREWVLTVCMDTAVDAALPTVDEARGTIYE-------GMFSSERKKCIVTGFPVY--DELVINDVPANKRDWNLFVSKTKQCPWTGKSENPQW 1772          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: A0A7S2HGU3_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2HGU3_9STRA)

HSP 1 Score: 758 bits (1957), Expect = 5.650e-251
Identity = 467/1065 (43.85%), Postives = 615/1065 (57.75%), Query Frame = 2

Query:  977 LRGGMPAQAGRVLKERSVENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVRRAVRPGCGRGRGRGSNCGGGPVRACRQ-------------A*ESITVL*RGRHVEGCASCMPET------------FAPPRAQSPRAVPG------CSGL---DRQRWCQG---------------GLPQRWPRSGAKPGLGCRYRCLPQGYPECDDERRRPGGGVGEGHHGGQGRTAKQAHGGSARGVRPPGRHREARGGGGGAEGGRPAXXXXXXXXXXXRV---GKGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDEASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDREASARPGAQETVERLLRILQSQTQNLKASNKAAPAGFEHLLMATHYTCLMGRC----REKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEKEASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQVNHARANKRDWNAYVRIFRQCPWTGLDATPQY 4003
            L+GG+PA+A  V+K  ++ N   L+E VA+ L+++G+HD+AGEFYE M+QLQRA+DSY +GNA+RQAVELARR F ++VV LQE WGDYL++ KQVDMAINHYIE   + KA+ A L SRQW KAAQL+E L+ +  + + + +A+H EE+     AE+F+  A +PQLAV+MYT+ N W+ AH+LA+++M+E EV +LY+ QAE+M   G L EAE+L+L V E DLA+ MYKK +++D+MVRLVA++R ++LKETH +LAQ LE EG+L++AEHHY EAGEWLSAVNMYRS++MW++A+RVAK +GG +A KRVAYAWALALGG+AGAKLL K GLI+PAI+YA ESGAFDHA ELA+    +KLP IHLKHAL+LEDEE++ EAE+EF+ A KPREAIDM+VHQ+ W +A RVAE +DP  V               ++      RAC               A +    L   +  +  +  +  T             A   AQ+ R   G       +G    + ++W                   L + W  +       CR R     YPE   E  R                           +R   RH  A      AE  R A                 K   + QG   L E+V+KAY+ +LM    T  L++MG T+AALD+ A+  +W++LW  A K   G + LAKYA +RV+ +L D           G    LDDAV+ LQ  GAP                          +MY  L KAILGR +E        ET+  L  I    T N++ S        E LLMA HY  +   C    R+    D   LA K +ITLLRY+ +IP DKCFY A    G  CK+    NLAFVLLNRYVD TEAIDEG +S +DNSDFA A+NVP  +   LP + Y+  E  REEAR+WVLSVCMD  ++Q LP E  A GT+Y       AGLY  +L +C+VTGFP+  RD +++N   ANK+DWN  V   ++CPWT    +PQ+
Sbjct:   64 LKGGLPAKAANVIKSHNMTNNSHLVERVATALASSGLHDRAGEFYEMMDQLQRALDSYLKGNAYRQAVELARRHFGSQVVSLQEQWGDYLVSSKQVDMAINHYIEANCSTKAIEAALNSRQWLKAAQLVEYLDADKRKAYFKRIAQHFEESEQLEEAEKFYKLAGSPQLAVKMYTENNQWEKAHQLAITFMTEREVAMLYISQAERMIGLGKLKEAERLFLAVKEPDLAINMYKKQRKYDSMVRLVAQYRKELLKETHLFLAQHLESEGNLREAEHHYCEAGEWLSAVNMYRSNNMWDEAIRVAKIYGGVNASKRVAYAWALALGGEAGAKLLTKLGLIDPAIDYAMESGAFDHAFELARSSLQSKLPDIHLKHALYLEDEEKYPEAEDEFINAQKPREAIDMYVHQQDWVNAMRVAETYDPSAV---------------ADVYVYEARACADQKRYARAEELFLAASKPELALTMYKEAQMWSEALELTTQHLPHKLGEVNMAYQAAQASRGTGGTKTDFLANGRMWENSKQWSNAVDAYLNARKDVLPPDELEEIWECAVRVARTECRVR-----YPEVVMEVTRR--------------------------LRDVRRHETA------AEILREAQQIEAAVDCAMDGECWDKARELAQGQGMLQERVQKAYESYLMSRNNTAGLMEMGHTDAALDVYAKNRDWEKLWAQADKSEVGQDVLAKYAAIRVKQLLSD-----------GVNAGLDDAVVTLQTYGAPEHEEYY--------------------EMYANLTKAILGRSQEQENEVDQSETLGMLRNIFMRLTTNIRLSYGQCDPVLEQLLMACHYMNVFRTCTAEFRKSKNPDIGTLANKTAITLLRYNSVIPVDKCFYLA----GMSCKEQNEVNLAFVLLNRYVDWTEAIDEGDSSGIDNSDFASASNVPFTE--VLPGQHYLSDEDLREEAREWVLSVCMDDNVNQTLPDEGGAQGTIY-------AGLYMCDLQTCIVTGFPIAPRDELRMNKVVANKKDWNLLVSKTKKCPWTEEKESPQW 1032          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: A0A7S4E4W1_9STRA (Hypothetical protein n=3 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S4E4W1_9STRA)

HSP 1 Score: 744 bits (1920), Expect = 7.950e-237
Identity = 461/1042 (44.24%), Postives = 618/1042 (59.31%), Query Frame = 2

Query:  977 LRGGMPAQAGRVLKERSVENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRV------RRAVRPGCGRGRGRGSNCGGGPVRACRQA*ESITVL*RGRHVEGC----ASCMPETFAPPRAQSPRAVPGCSGLDRQ---RWCQGGLPQRWPRSGAKPGLGCRYRCLPQGYPECDDERRRPGGGVGEGHH-----GGQGRTAKQAH---GGSARGVRPPGRHREARGGGGGAEGGRPAXXXXXXXXXXXRV---GKGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDEASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDREASARPGAQETVERLLRILQSQTQNLKASNKAAPA---------GFEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEKEASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQVNHARANKRDWNAYVRIFRQCPWTGLDATPQY 4003
            L+GGMP+ A R++K  +V+N   ++E VA++ ++ G++++AGEFY+  +QLQRAMD++ +G++FR+AVELAR+ FP  VV+L E WGDYL+   QVDMAINHYIE   ++KA+ A LT RQW++A QLLE++E   A+P+L  LA H+E+  +   AERF+V A AP+ AVEMYT+ N WD AHK+A SYM   EV +LY+ QA+ +E  G+L +AEKLYL + E DLA+ MYKK +++DAMV+LV KHR ++LKETHQYLAQ LE EG+L+DAEHHY EA EWLSAVNMYR++D+W +A+RVA  HGG +A KRVAYAWAL+LGGDAGAKLL KQGLIEPAI+YA ESGAFDHA ELA+  CP KL  +HLKHAL+LEDEER+KEAE EF+QA KPREA+DM++HQ+AW+DA  VA  +DP         +  V+   G+                 Q  E + +L     +       A    E  A      P  +   S    Q   R   GG    +  +G +     ++      Y     E  +  G + E  +       Q     + H      +R +R  GRH  A      AE  R +                 K   +  G ++   +V+ AYQ  L   E T+ L ++G   AA+DIL ++ EWD+LW+    +G      AKYAGL+   +  D          G N   L  AV  L   GAPP  S +                     MY  LV A+LG+D         Q+  + L+  L++   +L  SNK             GFE LLMATHY  L   C + G K    +A K+S+TL+RY+ +IP DK FY A    G++ +D G++NLAF+LLNRY+DLTEAIDE +   +DN+DFA+ATN+P   +  LP KQY+  E +REE RDWVLS CMD  IDQ LP  K + GT+Y       AGLYAS+LP+C+VTG PV K +L+QVN++ ANK DWN +VR  + CPWT ++  P Y
Sbjct:  796 LQGGMPSHAVRLIKHNNVDNSPAIVEQVAASFTSGGLYEQAGEFYQFADQLQRAMDAFLKGSSFRKAVELARKHFPGRVVELHERWGDYLIENNQVDMAINHYIEASLSSKAIEAALTCRQWTRAIQLLESVECSMAQPYLCRLAHHYEDIDDTREAERFYVAAGAPEKAVEMYTRVNLWDCAHKVASSYMEPREVSLLYISQAQHLEAEGNLKDAEKLYLTIDEPDLAINMYKKQRKYDAMVQLVEKHRQELLKETHQYLAQHLESEGNLRDAEHHYCEANEWLSAVNMYRTNDIWEEAMRVATMHGGPNASKRVAYAWALSLGGDAGAKLLTKQGLIEPAIDYAVESGAFDHAFELARSACPEKLSDVHLKHALYLEDEERYKEAELEFIQARKPREAVDMYIHQQAWSDALAVANTYDPTAASDIYISQARVKVDAGK----------------YQHAEELFLLAAKPELALSMYKDAGMWVEALALAERHLPHMLSNVSLAYSQAEARRGTGGSKINFISTGQQLEQKGQWDSAVDAYLNARQELIKDPGDLEEIWYCAVAVARQHMPTNRCHDIVADVSRKLREIGRHETA------AELLRESNDLRGAIECAIEGRCWAKARELAIGSSEFEAEVDLAYQAALRSAEDTDGLFELGHRAAAMDILVERREWDKLWQKIDYEGFNFSVRAKYAGLQAAQITSD----------GTN---LIMAVHTLNQHGAPPPGSNM--------------------KMYRALVVAVLGQDHSQVHLD--QKHHKTLVSELRNVLFDLGRSNKETQHDALGVHTSDGFEQLLMATHYYNLYLTCMQHGLKG---IALKISVTLIRYAGIIPIDKVFYLA----GTIARDQGHDNLAFLLLNRYIDLTEAIDEESIGNIDNADFADATNIPFPFD--LPSKQYLVEEEDREEIRDWVLSTCMDKSIDQQLPGSKLSLGTVY-------AGLYASDLPTCIVTGSPVQKWELLQVNNSIANKVDWNQFVRKVKLCPWTQVEQNPIY 1764          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: F0YKY3_AURAN (Uncharacterized protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YKY3_AURAN)

HSP 1 Score: 721 bits (1861), Expect = 2.850e-228
Identity = 457/1037 (44.07%), Postives = 612/1037 (59.02%), Query Frame = 2

Query:  977 LRGGMPAQAGRVLKERSVENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVRRAVRPGCGRGRGRGSNCGGGPVRACRQA*ESITVL*R------------GRHVEGCASC---MPETFAP-----PRAQSPRAVPGCSGLD----RQRWCQGGLPQRWPRS-----GAKPGLGCRYRCLPQGYPECDDERRRPGGGVGEGHHGGQGRTAKQAHGGSARGVRPPGRHREARGGGGGAEGGRPAXXXXXXXXXXXRV---GKGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDEASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDR----EASARPGAQETVERLLRILQSQTQNLK--ASNKAAPAGFEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEKEASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQVNHARANKRDWNAYVRIFRQCP 3973
            LRGGMP +A R++K+  + N   +LE V+++L+A G+H++AGEFYE M+QLQRAMD+Y +G +FR+AVELAR+ FP  VV+LQE+WGDYL    QV+MAINHYIE   ++KA+ A L++RQW+KAAQ+LE ++ + A+P+LR LARH+E++ +   AERF+V A AP  AVEMYT+AN WD AHK+A SYM   EV +LY+  A+K+E  G+L +AE+LYL V E DLA+ MYKK +++DAMV+LV KHR ++LKETHQYLAQ LE EGSL+DAEHHY EA  W S                           KRVAYAWAL+LGGDAGAKLL KQGLIEPAI+YA ESGAFDHA ELA+  CP KLP +HLKHAL+LEDEER+KEAE EF+QA KPREAIDM++HQ+AWA+A  VA  +DP     +  P       R     G    A     E + +L              G   E  A     +P   A       +A++ R   G   +D     Q+W Q    ++W R+      A+PGL    + L + +    D  RR         H    +        + + +R  GRH  A      +E  R                   K   +  G + L  +V+ AYQ  L   E T+ LL++G   AALD+LA + EWDRLW+ A ++       AKYAGL+   ++              ++ +L  AV  L+  GAPP                     GP   MY  LV A+LG++     E  A   +   +  +L  L S  Q  +  A   +   GFE LLM TH+  L   C + G KD   +A K+SITL+RYS LIP DK F+QA    G + +D G++NLAFVLLNRY+DLTEAI+EG+   +DN+DFA+ATNVP      LP++QY+P E +REE RDWVLS+C+D  +DQ LP +++A+GT++       AGLYAS+LP+C+VTG+PV K +L+ VN++ ANK DWN YVR  ++ P
Sbjct:  761 LRGGMPGRAARLVKQHGINNPPSILERVSASLTAGGLHEQAGEFYERMDQLQRAMDAYLKGASFRKAVELARKHFPGRVVELQELWGDYLFEHNQVEMAINHYIEASMSSKAIDAALSARQWTKAAQMLENVDLDIAQPYLRRLARHYEDSNDTSEAERFYVAAGAPDKAVEMYTRANLWDRAHKIASSYMEPREVSLLYISHAQKLEAEGNLKDAEQLYLTVDEPDLAINMYKKQRKYDAMVQLVEKHRRELLKETHQYLAQHLESEGSLRDAEHHYCEAAPWWS---------------------------KRVAYAWALSLGGDAGAKLLTKQGLIEPAIDYAIESGAFDHAFELARSACPGKLPDVHLKHALYLEDEERYKEAEAEFIQANKPREAIDMYIHQQAWAEALAVANKYDP-----SASPDVYVSHARAEADAGQHQHA-----EELFMLAAKPELALSMYRDAGMWTEALALAQRHLPHQLAEVSLAYSQAEAQRGTGGTK-VDFLSAGQQWEQ---QKQWGRAIEAYLNARPGLLEDPKELEEIWECAIDVARR---------HMSPEKLRDIVTNVTLK-LREIGRHEAA------SELLRELNDIDGAVRCAMEGRCWAKARELAIGSSTLEAEVDAAYQSALRSAEDTDGLLELGHRTAALDVLADRKEWDRLWQMADREQIHFSVRAKYAGLQAAQIV-------------ASKGDLIQAVRTLKQHGAPPP--------------------GPNIQMYRDLVMAVLGQNHANPPEQDAHINSVSELRDILFSLGSSHQGTQHDALGISGANGFEQLLMTTHFYNLYLICTKHGLKD---IALKVSITLIRYSGLIPIDKAFHQA----GIMARDQGHDNLAFVLLNRYIDLTEAIEEGSIDNIDNADFADATNVPFPFE--LPLQQYLPREDDREEIRDWVLSICVDKAVDQQLPAKQQAAGTVH-------AGLYASDLPTCIVTGYPVQKWELLNVNNSIANKVDWNQYVRKVKKWP 1691          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: A0A0P1AMT9_PLAHL (Intraflagellar transport protein 172 n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AMT9_PLAHL)

HSP 1 Score: 607 bits (1565), Expect = 1.940e-185
Identity = 418/1092 (38.28%), Postives = 584/1092 (53.48%), Query Frame = 2

Query:  977 LRGGMPAQAGRVLKERSV-ENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVRRAV---------RPGCGRGRGRGSNCGGGPVRACRQA*ESI----TVL*RGRHVEGCASCMPETFAPPRA-------QSPRAVPGCS-GLDRQRWCQ----------------GGLPQRWPRSGAKPGLGCRYRCLPQGYPECDDERRRPGGGVGEGHHGGQGRTAKQAHGGSARGVRPPGRHREARGGGGGAEGGRPAXXXXXXXXXXXRVGKGPGIGQGHAQLSE----KVEKAYQQHLMRGEATEELLQM---------------------------------GQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDEASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDREASARPGAQETVERLLRILQSQTQNLKASNK----AAPAGFEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEK--EASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQVNHAR--ANKRDWNAYVRIFRQCPWTGLDATPQY 4003
            L+GG+PA+A ++L +R++  +  QL+ETVA  L +AGM +KAG+ +E M Q  RA+ +Y + NAFR+AVEL+R+ FP +V+ L+E WGDYL++QKQ+DMAINHYIEG    KAV A L SRQW+KA+QL+ETLE + + P+ R LARH++EAGN   AER F+ A+A + AVEMYT+AN WDAA+++AL+++ + E   LY+ QA +ME AG L EAEKL+L V E DLA+ MYK  K ++ M+RLV K+R D+LK+TH YLAQQLE EG+ K+AEHH+AEAGEW SAVNM+RS+DMW++A+RVAKFHGG +A KRVAYAWA+ LGG+ G KLL + GLIEPAI+YA ESGAF+HA ELA+ C   KLP +HLKHALFLEDEERFKEAEEEF++AGKPREA+DM+VHQ+ W +A RVAE  DP  V             R    R  G   + G  P  A     E+      V    RH+      M    A  RA       +    +  C   +  Q++ Q                G L + W ++     +    +  P  Y    +E                   A +  G S        RH ++                        RV   P   +   QL+     ++E+A QQ    G AT    +                                  G+ ++AL+   Q+GEWD++  +AAK     E+LAKY  LR   + +                E   A+  + D G P  +  L                    DM E LV+ +L  D    A    Q  ++ L++ L+   ++L+ + K    +     E  L+ THY  L     +    +  ++ AK+S++LLR+ D++PADK FY A    G   +     + AFV  NRY+DL EAID+G AS LDN+DF   T++P   +  LP   Y+  ES REE RDWVL++ MD ++ + LP         ++Y+A +        SE  SC++TGFPV  + ++     +  A++  WN +++ F  CPW        Y
Sbjct:  779 LKGGLPAKAAQLLNQRNLGRDHKQLMETVADALYSAGMFEKAGDQFERMEQESRALAAYIKANAFRKAVELSRKHFPDKVLRLEEAWGDYLVSQKQMDMAINHYIEGNVPTKAVEAALNSRQWAKASQLVETLEDDVSLPYFRRLARHYQEAGNLEQAERCFIKADAARDAVEMYTRANKWDAAYQVALNHLDKYETERLYVEQAHRMERAGKLKEAEKLFLTVNEPDLAINMYKNHKNYEQMIRLVTKYRKDLLKDTHMYLAQQLEHEGNYKEAEHHFAEAGEWQSAVNMFRSNDMWDEAIRVAKFHGGINASKRVAYAWAMELGGEQGGKLLTRLGLIEPAIDYAVESGAFEHAFELARNCASKKLPEVHLKHALFLEDEERFKEAEEEFIKAGKPREALDMYVHQQDWQNAMRVAETADPASVADVFLAQARLWVERKEYQRAEGFFLSAGK-PEMALAAYLEAAMWVDAVQIAKRHLPH--KLMEVNMAHQRAIFSGGPKKKEELIEACEMWVASQQYVQAIDAYLSITINQLSDLGELEEVWTKA-----IELCAKHDPSRYKSIVEE------------------VASRLLGMSC--FDAAARHYQS------------IDMMNEALDCFLRVNNWPAAQKLCEQLAPELLPRLERA-QQASAFGSATHHSAEAKMTGSSYTPSADIKVQDIPEKKESKHNIESDGAGRGSSALEAWMQRGEWDKVLSSAAKHSR--ESLAKYLVLRCSRLCE--------------HNETATAIRTISDYGIPLESDSL--------------------DMVENLVQKVLASDHTIEANTDHQTALQELIKCLRKLVKDLRTNGKEFLKSRVQKIEQWLLVTHYFVLK---HQAANAELDDVVAKISMSLLRFVDVLPADKMFYLA----GVATRKKKWLSAAFVYFNRYLDLCEAIDDGDASNLDNTDFI-GTDIPSPLDFALPEVHYLADESAREEIRDWVLTISMDQQVAEKLPERACLNCKASIYEASLQCSECKATSE--SCIITGFPVAAKMIVHCATCKVVADREMWNKWIKQFGNCPWCSAPQKMSY 1783          

BLAST of mRNA_P-lacustris_contig1017.246.1 vs. uniprot
Match: H3GS66_PHYRM (Uncharacterized protein n=27 Tax=Peronosporaceae TaxID=4777 RepID=H3GS66_PHYRM)

HSP 1 Score: 606 bits (1563), Expect = 3.950e-185
Identity = 424/1077 (39.37%), Postives = 585/1077 (54.32%), Query Frame = 2

Query:  977 LRGGMPAQAGRVLKERSV-ENQGQLLETVASTLSAAGMHDKAGEFYEEMNQLQRAMDSYTRGNAFRQAVELARRSFPAEVVDLQEMWGDYLMTQKQVDMAINHYIEGRANAKAVGACLTSRQWSKAAQLLETLEGESARPHLRTLARHHEEAGNYPLAERFFVDAEAPQLAVEMYTKANHWDAAHKLALSYMSEGEVRVLYMGQAEKMEVAGSLLEAEKLYLQVGEVDLAVAMYKKAKRFDAMVRLVAKHRPDVLKETHQYLAQQLEMEGSLKDAEHHYAEAGEWLSAVNMYRSSDMWNDALRVAKFHGGQSAYKRVAYAWALALGGDAGAKLLNKQGLIEPAIEYATESGAFDHALELAQVCCPAKLPGIHLKHALFLEDEERFKEAEEEFLQAGKPREAIDMFVHQKAWADACRVAEGHDPRRVRRAV---------RPGCGRGRGRGSNCGGGPVRACRQA*ESI----TVL*RGRHVEGCASCMPETFAPPRA-------QSPRAVPGCS-GLDRQRWCQ----------------GGLPQRWPRS------------------GAKPGLGC-RYRCLPQGYPECDDERRRPGGGVGEGHHGGQGRTAKQAHGGSARGVRP---PGRHREARGGGGGAEGGRPAXXXXXXXXXXXRVGKGPGIGQGHAQLSEKVEKAYQQHLMRGEATEELLQMGQTNAALDILAQKGEWDRLWEAAAKQGSGAETLAKYAGLRVRSVLDDEASWEKQVSGGGNRRELDDAVLVLQDKGAPPITSGLGRSSSAVGGGVPSGSGGPAADMYERLVKAILGRDREASARPGAQETVERLLRILQSQTQNLKASNKAAPAG----FEHLLMATHYTCLMGRCREKGGKDCLELAAKLSITLLRYSDLIPADKCFYQASTRAGSLCKDLGNENLAFVLLNRYVDLTEAIDEGTASLLDNSDFAEATNVPLVDNSTLPIKQYIPVESEREEARDWVLSVCMDAKIDQALPPEK--EASGTLYQARMPKPAGLYASELPSCVVTGFPVHKRDLIQVNHAR--ANKRDWNAYVRIFRQCPWTGLDATPQY 4003
            L+GG+PA+A ++L +R++  +  QLLETVA  L +AGM +KAG+ +E+M Q  RA+ ++ + NAFR+AVEL+R+ FP +V+ L+E WGDYL++QKQ+DMAINHYIEG    KAV A L SRQW+KA+QL+E LE + + P+ R LARH+++AGN   AER F+ A+A + AVEMYT+AN WDAA+++AL+++ + E   LY+ QA +ME AG L EAEKL+L V E DLA+ MYK  K ++ M+RLV K+R D+LK+TH YLAQQLE EG+ K+AEHH+AE+GEW +AVNMYRS+DMW++A+RVAKFHGG +A KRVAYAWA+ LGG+ GAKLL + GLIEPAI+YA ESGAF+HA ELA+ C   KLP +HLKHALFLEDEERFKEAEEEF++AGKPREA+DM+VHQ+ W +A RVAE  DP  V             R    R  G   + G  P  A     E+      V    RH+      M    A  RA       +    +  C   +  Q++ Q                G L + W ++                   A   LG  R+    + +   D         +   +     +  +Q          P   P   R  +    G+ G  PA                P         SEK E      L  G    +    G+  +ALD   Q+GEWD++  +AAK G G  +LAKY  LR   + + +              E+D A+  + D G P     L                    DM E+LV+  LG           Q  +  L++ L+   ++L+A+ K  P+      E  L+  H+  +  +    G  D   L AK+S++LLR+  ++PADK FY A T A    K L   + AFV  NRY+DL EAID+G AS LDN+DF   T++P   +  LP   Y+  ES REE RDWVL++ MD ++ + LP         ++Y+A +       ASE  SC++TGFPV  +  +     +  A++  WN +V+ F  CPW        Y
Sbjct:  779 LKGGLPAKAAQLLNQRNLGRDHKQLLETVADALYSAGMFEKAGDQFEKMEQESRALAAFIKANAFRKAVELSRKHFPDKVLRLEEAWGDYLVSQKQMDMAINHYIEGNVPTKAVEAALNSRQWAKASQLVENLEDDVSLPYYRRLARHYQDAGNLEQAERCFIKADAARDAVEMYTRANKWDAAYQVALNHLDKYETERLYVEQAHRMERAGKLKEAEKLFLTVNEPDLAINMYKNHKNYEQMIRLVTKYRKDLLKDTHMYLAQQLEHEGNYKEAEHHFAESGEWQAAVNMYRSNDMWDEAIRVAKFHGGINASKRVAYAWAMDLGGEQGAKLLTRLGLIEPAIDYAIESGAFEHAFELARNCATKKLPEVHLKHALFLEDEERFKEAEEEFIKAGKPREALDMYVHQQDWQNAMRVAESADPASVADVFIAQARLWIERKEHQRAEGFFLSAGK-PELALAAYLEAAMWVDAVRIAKRHLPH--KLMEVNMAHQRAIFSGGPKKKEELMEACEMWVASQQYVQAIDAYLSISVDQISDLGELEELWAKAIELCAKHDPSRYKSIVEEVASRLLGMSRFDSAAEHFQSIDKMNEALDCFLRVNNWAAAQKLCEQ--------HAPELLPRLERAQQASAFGSAGHHPAEAKMAGSSYT------PSADAKVQYASEKKESK----LSAGGGDSD--DAGRGTSALDAWMQRGEWDKVLSSAAKHGPG--SLAKYLVLRCARLCEHD--------------EMDAAIKTIADYGIPLDPDAL--------------------DMTEKLVQKTLGCAHGIEDNAEHQAVLPELVKCLRKLVKDLRANGKDFPSSRVQKMEKWLLVAHFFAIKHQASSAGLDD---LVAKISMSLLRFVGVLPADKMFYLAGT-ASRKKKWL---SAAFVYFNRYLDLCEAIDDGDASNLDNTDFI-GTDIPSPLDFALPESHYLAEESAREEIRDWVLTISMDQQVAEKLPERACLNCKASIYEAALQCSECKTASE--SCIITGFPVAAKTTVHCATCKVIADRETWNKWVKQFGICPWCSAPQKMSY 1786