mRNA_P-lacustris_contig1015.236.1 (mRNA) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: D8LI50_ECTSI (MFS domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LI50_ECTSI)

HSP 1 Score: 807 bits (2085), Expect = 3.770e-287
Identity = 458/664 (68.98%), Postives = 514/664 (77.41%), Query Frame = 1

Query:   13 GERQALFRPNSPGYS-SNFG--HGSFSSK------QQTAPSITVPSEEYLDLTISGGKGSS------------SEFRGEVRATLDDKASLAIVLGDVLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGRTLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSVFSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPLLGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATDIGSDVD-ISSADEDALNQGQTGQWPASEYFPLDSISS-SGSLPRRGSSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGR------VYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAFPPSLTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALFLFLVGILSVTFVLLFAFRKPYLDCVKVTDRRKVAAAPASPPTGGAVK 1917
            GERQAL  P++ G   S+ G  HGS  S       +Q +P+ITVPS++                         + FRGEVR TLDDKAS A+VLGDVLLVDTARGIFFPTLW H++QLGGDKIALGYCVGAFSLGRTLMSPVFGRMST+HGYRRALLIST LV FG  FY VADSVF+VFLSQVFLGVGSGTLGVTRGYVADKTT EQRTY LAY TAVQYAGFTVMP LGG FSYLLGS EIPLLGRFL LTQFTAPAFF+ A+ST +F+L++FVFQDGI  +PA+  SS+KELSG ASGVTL TD+GSD     S D+D  NQ Q   WPA+EY P  S+SS SG    RGS   SVVG YV+IS D    GSDD K  EDVE GYQTGLAA AA +        V  + +DS ++EA+ G+V    + TLGEG   WLARKLR+ LPSRGDMLIYGGFLLNVSTKGTI+CFETIGA YA+T FSLTSAEAGS+FATCGAIGV ALLSMRLLCK FNDIQLVLGGMSLMI+TC +LA PP+  GL+VF+ AVFL+YSVGYPIGHTAVLGLFSKVVGAQPQG LMGWFGSAGA+ART FPVL GVL Q  G++ALFLFLV +LSVTF LLF FR+PYLDC+ +T+RRK A   A+PP+ GA K
Sbjct:    6 GERQALLFPSTGGGGGSSIGGRHGSTRSSSNSHKIKQGSPTITVPSDKXXXXXXXXXXXXXXXXXXXXXXXXXTSFRGEVRPTLDDKASFAVVLGDVLLVDTARGIFFPTLWTHVSQLGGDKIALGYCVGAFSLGRTLMSPVFGRMSTEHGYRRALLISTALVSFGTLFYAVADSVFTVFLSQVFLGVGSGTLGVTRGYVADKTTKEQRTYLLAYTTAVQYAGFTVMPSLGGFFSYLLGSTEIPLLGRFLMLTQFTAPAFFVAAMSTVLFLLLWFVFQDGIEQKPAKTTSSAKELSGTASGVTLTTDLGSDDSGCLSDDDDVPNQEQQDLWPAAEYSPPGSVSSDSGGRTGRGSPP-SVVGQYVDISRDGGWGGSDD-KGMEDVEDGYQTGLAAAAAGAAAAGNRAGVQATFHDSVQHEAKHGDVVGRETATLGEG--GWLARKLRLRLPSRGDMLIYGGFLLNVSTKGTISCFETIGAAYAITTFSLTSAEAGSVFATCGAIGVVALLSMRLLCKHFNDIQLVLGGMSLMIVTCAMLALPPTFIGLNVFIAAVFLMYSVGYPIGHTAVLGLFSKVVGAQPQGALMGWFGSAGALARTCFPVLAGVLCQVFGTSALFLFLVALLSVTFALLFTFRRPYLDCIDITNRRKAAVGAANPPSHGAGK 665          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: D7FUX3_ECTSI (MFS domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUX3_ECTSI)

HSP 1 Score: 719 bits (1855), Expect = 1.630e-253
Identity = 389/567 (68.61%), Postives = 448/567 (79.01%), Query Frame = 1

Query:  214 SLAIVLGDVLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGRTLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSVFSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPLLGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATDIGSDVDISSADEDALNQGQTGQWPASEYFPLDSISSSGSLPRRG-SSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAFPPSLTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALFLFLVGILSVTFVLLFAFRKPYLDCVKVTDRRKVAAAPASPPTGGA 1911
            S +IVLG++LL+DTARGIFFPTLW H+ QLGGDKIALGYCVGAFSLGRTL+SPVFGRMST+HGYRRA LIST L+ F + FY VADSV +VFLSQ+FLG+GSGTLGVTR YVADKTT EQRTY LAY T+VQYAG TVMP+LGG FSYLLGS EIPLLGRFL LTQFTAPAFF+ A+ST +F+L++ VFQDGI  +PA+  SS KELSG ASGVTL TD+GSD     +D D   Q Q   WPA+E  P  S+S+S S  R G  S  SVVG YV+ S D    GSDD K    VEGGYQTGL +    +G V  + + S E EA+ G+V    + TLGEG   WLAR L + LPSRGDMLIYGGFLLNVSTKGTI+CFETIGA YA+ NFSLT+AEAGS+FA CGAIGV  LLSMRLLCK FNDIQLVLGG+SLMI+TC +LA PP+  GLHVF+ AVFL+YS+GYPIGHTAVL LFSKVVGAQPQGTLMGWFGSAGA++R  FPVL GVL +  G++ALFLFL  +LSVTF L+F FR+PYLD ++V +RRK A   A+PP  GA
Sbjct:   14 SRSIVLGNLLLIDTARGIFFPTLWTHVIQLGGDKIALGYCVGAFSLGRTLLSPVFGRMSTEHGYRRAFLISTVLIPFASLFYAVADSVLTVFLSQLFLGMGSGTLGVTRAYVADKTTKEQRTYLLAYTTSVQYAGLTVMPILGGFFSYLLGSTEIPLLGRFLLLTQFTAPAFFVAAMSTVLFLLLWSVFQDGIEQKPAKTSSSGKELSGTASGVTLTTDLGSDDSGGLSDGDVPYQEQQDLWPAAEVSPPGSVSNSSSGGRTGRGSPPSVVGQYVDTSGDGDSGGSDDDKGMGHVEGGYQTGLGSAGNRAG-VQAAFHVSAEDEAKHGDVVGRETSTLGEG--GWLARNLWLRLPSRGDMLIYGGFLLNVSTKGTISCFETIGAAYAIINFSLTNAEAGSIFAACGAIGVVTLLSMRLLCKHFNDIQLVLGGVSLMIVTCAMLALPPTFIGLHVFIAAVFLVYSIGYPIGHTAVLALFSKVVGAQPQGTLMGWFGSAGAISRASFPVLAGVLCEVFGTSALFLFLGALLSVTFALVFTFRRPYLDWIEVPNRRKGAVGAANPPPHGA 577          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: D8LI51_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LI51_ECTSI)

HSP 1 Score: 530 bits (1365), Expect = 2.630e-181
Identity = 300/470 (63.83%), Postives = 346/470 (73.62%), Query Frame = 1

Query:  511 VGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPLLGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATDIGSDVDISSADEDALNQGQTGQWPASEYFPLDSISSSGSLPRRG-SSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAFPPSLTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALFLFLVGILSVTFVLLFAFRKPYLDCVKVTDRRKVAAAPASPPTGGAVK 1917
            +GSGTL VTR YVADKTT EQRTY LAY  + QYAGFTVMP+LGG FSYLLGS EIPLLGRFL LTQFTAPAFF+  L                  +PA+  SS KELSG ASGVT  TD+GSD     +D D  +Q      PA+EY P  S+SS+ S PR G  S  SVVG YV+ S D    GSDD K    VEGGYQTGL +    +G V V+ +DS ++EA+ G+V    + TLGEG   WLARKLR+ LPSRGDM IYGGFLLNVSTKGTI+CFETIGA YA+T FSLT+AEAGS+FA CGAIGV  LLSMRLLCK FNDIQLVLGG+SLMI+TC +LA PP+  GLHVF+ AVFL+YS+GYPIGHTAVL LFSK+VG QPQGTLMGWFGSAGA++R  FPVL GVL + LG++ALFLFLV +LSV   LLF FR+PYLDC+++T+RRK A   ASP   G  K
Sbjct:    1 MGSGTLKVTRAYVADKTTKEQRTYLLAYTRSQQYAGFTVMPILGGFFSYLLGSTEIPLLGRFLLLTQFTAPAFFVAELK-----------------KPAKTTSSGKELSGTASGVTPTTDLGSDDSGGLSDGDVPDQEHQDLRPAAEYSPPGSVSSNSSGPRTGRGSPPSVVGQYVDTSGDGDSGGSDDDKGMGHVEGGYQTGLGSAGNRAG-VEVTFHDSVQHEAKHGDVVGRETSTLGEG--GWLARKLRLRLPSRGDMHIYGGFLLNVSTKGTISCFETIGAAYAITTFSLTNAEAGSIFAACGAIGVVTLLSMRLLCKHFNDIQLVLGGVSLMIVTCAMLALPPTFIGLHVFIAAVFLVYSIGYPIGHTAVLALFSKMVGTQPQGTLMGWFGSAGAISRASFPVLAGVLCEVLGTSALFLFLVAMLSVPIALLFTFRRPYLDCIEITNRRKAAVGAASPLARGVDK 450          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: A0A6H5KUL2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KUL2_9PHAE)

HSP 1 Score: 477 bits (1227), Expect = 2.500e-156
Identity = 288/606 (47.52%), Postives = 384/606 (63.37%), Query Frame = 1

Query:  163 SSSEFRGEVRATLDDKASLAIVLGDVLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGR---TLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSVFSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPLLGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATD--------------IGSDVDISSADEDALNQGQTG-QWPASEYFPLDSISSSGSL-------PRRGSSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDG---EVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAF-PPSLTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALFLFLVGILSVTFVLLFAFRKPYLDCVKVTDRRKVAAAPAS 1893
            SS +  G+V  TLDDKASL IV+  V L DTARGIFFPTLW ++  +GGD+I LGY VGAFS GR    ++SP FG +ST+HGYR+ L++++ L+  GA  Y +A +  ++  +Q+ +GVGSGTLGVTR YVADK+T EQRTY LAY TAVQYAGFT MP +GG  S+L+    IPLLG FL LT FTAPA+ ++  +  +F L+  VF+D I    + + S   + S   S   + T+              +G+  ++S A    ++   T  QWP +E    +  S  G           R  ST+S        +              +D E G    LAA + +    Y S+   EE    +G   E+R+E           W  R      P+  ++LIYGGFLLN+STKGTIACFET+GAEYAMT+F +TSAEAGS FAT G+IGV +LLSMRL+C+  ND+Q+VLGGM +MI+ C++    P   +GL +F+ AVFL+YSVGYPIGHTAVLGLFSKVVGAQPQG L+GWFGSAG++AR  FPV+ GV+SQ+LG++ LF+ LVG LS+T  +LFAFRK YLDCV++T ++ ++   A+
Sbjct:  153 SSPDQTGKVSPTLDDKASLVIVMCVVFLGDTARGIFFPTLWKYVQAMGGDRITLGYSVGAFSFGRQVAVMISPYFGGLSTKHGYRKVLMVASGLIALGALAYALAWTTAALIGAQIIMGVGSGTLGVTRAYVADKSTPEQRTYLLAYTTAVQYAGFTCMPFVGGFLSFLIKDRRIPLLGEFLVLTSFTAPAYVMVVAALLLFTLLKLVFEDSIPKGKSTKTSKQVKASTKKSTPLVKTEAEIAYSSGSAAKYLMGAPSEVSLARSGWMSDSDTDDQWPPAEPGARNGSSGPGLFVDSDDEDETRRPSTVSATTPKTATAM------------VQDSEQGL---LAAPSGNDSNGYGSVPAPEEGAGEEGGEEELRREER---------WWCRT-----PTASEVLIYGGFLLNMSTKGTIACFETLGAEYAMTHFGMTSAEAGSTFATFGSIGVVSLLSMRLICRYLNDVQIVLGGMGVMIVACLMFVRNPEGASGLPLFLWAVFLMYSVGYPIGHTAVLGLFSKVVGAQPQGALLGWFGSAGSLARIGFPVVAGVISQKLGTSKLFMVLVGTLSITAAILFAFRKRYLDCVEITKQKSLSRQAAA 729          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: D8LGE0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LGE0_ECTSI)

HSP 1 Score: 457 bits (1177), Expect = 4.360e-151
Identity = 271/570 (47.54%), Postives = 365/570 (64.04%), Query Frame = 1

Query:  238 VLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGR---TLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSVFSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPLLGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATD--------------IGSDVDISSADEDALNQGQTG-QWPASEYFPLDSISSSG----SLPRRGSSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILA-FPPSLTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALFLFLVGILSVTFVLLFAFRKPYLDCVKVTDRRKVA 1878
            V L DTARGIFFPTLW ++  +GGD+I LGY VGAFSLGR    ++SP FG +ST+HGYR+ L++++ ++  GA  Y +A +  ++  +Q+ +GVGSGTLGVTR YVADK+T EQRTY LAY TAVQYAGFT MP +GG  S+L+    IPLLG FL LT FTAPA+ ++  +  +F L+  VF+D I      + S   ++S   S   + T+              +G+  D+S A    ++   T  QWP +E    +  S  G    S     +  LS V      +  +         +  D E G    LA  + ++   Y S+  ++E  A +G          GE        +     P+  ++LIYGGFLLN+STKGTIACFET+GAEYAMT+F +TSAEAGS+FAT G+IGV +LLSMRL+C+  ND+Q+VLGGM +MI+ C++    P   +GL +F+ AVFL+YSVGYPIGHTAVLGLFSKVVGAQPQG L+GWFGSAG++AR  FPV+ GV+SQELG++ LF+ LVG LS+T  +LFAFR+ YLDCV +T R+ ++
Sbjct:    4 VFLGDTARGIFFPTLWKYVQAMGGDRITLGYSVGAFSLGRQVAVMISPYFGGLSTKHGYRKVLMVASGVIALGALAYALAWTTAALIGAQIIMGVGSGTLGVTRAYVADKSTPEQRTYLLAYTTAVQYAGFTCMPFVGGFLSFLIKDRRIPLLGEFLVLTSFTAPAYVMIVAALVLFALLKLVFEDSIPNGKPTKTSKQVKVSTKKSTPLVKTEAEMAYSSGSAAKYLMGAPSDVSLARSGWMSDSDTDDQWPPAELGARNGSSGPGLFVDSDDEDETRRLSAVSATTTPTPST--------AAVRDSELGL---LATPSGNNSNGYGSVPAAKEGAAGEG----------GEXXXXXXXXRWWCRAPTASEVLIYGGFLLNMSTKGTIACFETLGAEYAMTHFGMTSAEAGSIFATFGSIGVVSLLSMRLICRYLNDVQIVLGGMGVMIVACLMFVRSPEGASGLPLFLWAVFLMYSVGYPIGHTAVLGLFSKVVGAQPQGALLGWFGSAGSLARIGFPVVAGVISQELGTSKLFMVLVGTLSITAAILFAFRRRYLDCVDITKRKSLS 552          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: A0A7S2V0P8_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V0P8_9STRA)

HSP 1 Score: 295 bits (755), Expect = 1.010e-88
Identity = 192/521 (36.85%), Postives = 279/521 (53.55%), Query Frame = 1

Query:  214 SLAIVLGDVLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGRTLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSVFSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPLLGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATDIGSDVDISSADEDALNQGQTGQWPASEYFPLDSISSSGSLPRRGSSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAFPPSLTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALF 1776
            S  ++   VL+ D ARG+ FPTLW  +  LGGD+   GYCV AFS GR   SP FG++ST+ GY+  LLI+  +   G   Y  A S + + + Q  +G G+GTLGVTR YVAD T++ QRT ++AY TA+QYAGFTV P LG  F+ LLG D     G F  +++F+APA+F +  +    +L+ F F + I T   +  S ++ +S                          N+   G    S     DS  S  S   +        G  +  ++   R  +  G SA        TG   +                            +  + +G+  W               ++  GF+ N++TKGTI  +ET+GAE+A+T     SA+ G +FA+ G +GV ALLSMR LC+RF D+ LV  G+ LM+++CV L +   +TG   F VA+F++YSVGYPIGHTA++GLFSK+VG +PQG L+GWFGS+G++AR  FP+L+G +++  G++ LF
Sbjct:   67 SFCVIAYMVLVGDAARGLMFPTLWPLVESLGGDRSTQGYCVAAFSFGRIFASPYFGQLSTEKGYKDVLLIAIAITVVGCLTYAHATSTWLLMVGQCVMGFGAGTLGVTRAYVADNTSSAQRTTYIAYITALQYAGFTVCPSLGAAFTLLLGDDTYSW-GPF-QVSKFSAPAYFHIGAALLGGLLLQFAFDNYIPTARQRAKSRARAVS------------------------IRNRRSAG----SRSVSFDSARSPRSPTVKSPMVQGSEGLELVATSPQQRDSASSGTSA--------TGSLTIT--------------------------NTSLVEDGLSMW-------------KFMLAWGFINNIATKGTIGAYETLGAEFAITALHFDSADTGFIFASFGFLGVLALLSMRYLCERFADVFLVFWGVFLMVVSCV-LFYLVHVTGTVGFSVAIFVMYSVGYPIGHTALIGLFSKIVGNRPQGALLGWFGSSGSLARIIFPLLSGYVAETFGNSELF 509          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: A0A7S3UZ09_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UZ09_HETAK)

HSP 1 Score: 290 bits (743), Expect = 1.210e-87
Identity = 196/533 (36.77%), Postives = 277/533 (51.97%), Query Frame = 1

Query:  184 EVRATLDDKASLAIVLGDVLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGRTLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSVFSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPLLGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATDIGSDVDISSADEDALNQGQTGQWPASEYFPLDSISSSGSLPRRGSSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAFPPSLTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGA--QPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALF 1776
            E  A + D  S  ++   V + DTARG+ FPTLW  +++LGG ++  G+ V AFS GR L SP FG+MST +GY++ L  +  ++  G F Y  A + + +   QV +G+GSGTLGVTR YVAD +T   RT  +AY TA+QY GFTV P +GGL SYLLG  E+ LL   L+LTQ+TAPA  + AL+     L+   F+D +   PA+R + ++  S  A            +  SS  ++ +N   T   PA+                          H  E+S   +RS                                                         + AW                   G ++NV TKG++AC+ET+G   A+ +F ++  +AG +F++CG +GV ALLSM+ L    +DI+LV  G  +M+ +  +L F  +  GLH F  AVFL+YSVGYPIGHTAV+G+FSK+VGA  Q QG+L+GWFGSAG++AR  FP+L GV+S E GS  +F
Sbjct:   38 EDAAMVKDWVSFLVIGFVVFIGDTARGLMFPTLWPLVSKLGGTELTQGFTVAAFSFGRVLSSPHFGKMSTDYGYKKVLTFAACVMIAGCFLYATAQNCYWLIAGQVVIGLGSGTLGVTRAYVADNSTKRSRTVLMAYLTALQYGGFTVTPCVGGLLSYLLGDRELSLLPG-LSLTQYTAPALLMAALALVAIALLAGPFRDYV---PAERAALARPPSRPA------------LPASSVADNLVNYKPTTPLPAA--------------------------HPEEVSTRLWRS---------------------------------------------------------MVAW-------------------GLIMNVVTKGSVACYETLGTIVAIGSFGMSKPDAGFLFSSCGFLGVFALLSMKCLTAWADDIKLVFYGCLVMVASATLL-FLDARIGLHGFFAAVFLMYSVGYPIGHTAVIGIFSKIVGARTQQQGSLLGWFGSAGSLARIIFPILCGVVSSEYGSNTVF 451          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: A0A836C8M5_9STRA (Major facilitator superfamily domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C8M5_9STRA)

HSP 1 Score: 286 bits (731), Expect = 2.980e-85
Identity = 209/559 (37.39%), Postives = 280/559 (50.09%), Query Frame = 1

Query:  199 LDDKASLAIVLGDVLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGRTLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSVFSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPL-----LGRFLTL--TQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQ--RPSSSKELSGAASGVTLATDIGSDVDISSADEDALNQGQTGQWPASEYFPLDSISSSGSLPRRGSSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAFPPSLTGLHV--FVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALFLFLVGILSVTFVLLFAFRKPYL 1842
            L D  SLA +L  VL+ DTARGI FPTL +++  +GG+K  LG  V  FSLGR + SP+ G+ STQ+G RR L  +   +  GA  Y +A S   +  +QV +G+GSGTLGVTRGYVAD+T   +RT  LA  TAVQY GF   P+LG +    +G           LG ++ L  +QFTAP + L   S AV +LV+   +D      A   RP   ++    A G  +A                L+Q                  + G +                                ED+EG      AA AA +       +D+                                 L    +  I G FLLNV+ KG IA +ET+GA YA+   SL+ A AG  FAT GA GV ALLSM  L +RFND+QL++GG  +M+L+C +L  P    GL    FV ++FL YS+GYPIGH+AVLG+FSK+VG + QG L G F SAG++AR  FP+  G ++Q LG   +F  L  +LSVT V+L  +R+ YL
Sbjct:   50 LADYKSLATILTVVLIGDTARGIVFPTLSSYVILMGGNKSTLGAAVAGFSLGRVIASPLLGKWSTQYGCRRVLAAALSALSLGAILYALASSTAWLIAAQVMMGLGSGTLGVTRGYVADRTPNARRTGMLAMLTAVQYGGFACTPLLGAMLCRAIGDGAWGFGHHFALGGWVRLEWSQFTAPGYLLAGASAAVALLVWLCLEDAAPAAKATPPRPQQQQKAVEGAVGEKMAL---------------LHQXXXXXXXXXXXXXXXQAVAYGDV--------------------------------EDLEG------AAAAARNHDAQQQNHDAP--------------------------------LTRSAEAAILGAFLLNVACKGVIATYETLGANYAINTLSLSPASAGLFFATFGAGGVVALLSMGALSRRFNDVQLIVGGAVVMLLSCALLVVPTQGGGLGYAGFVASIFLQYSIGYPIGHSAVLGMFSKIVGKRAQGALQGLFASAGSVARIAFPLAVGTVAQLLGVHVVFALLCVVLSVTIVILLVYRRTYL 523          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: A0A7S4AKJ6_9STRA (Hypothetical protein n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4AKJ6_9STRA)

HSP 1 Score: 273 bits (699), Expect = 5.350e-81
Identity = 199/577 (34.49%), Postives = 282/577 (48.87%), Query Frame = 1

Query:   88 KQQTAPSITVPSEEYLDLTISGGKGSSSEFRGEVRATLDDKASLAIVLGDVLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGRTLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSV----FSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPL-LGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATDIGSDVDISSADEDALNQGQTGQWPASEYFPLDSISSSGSLPRRGSSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAFPPS--LTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALFLFLVGIL 1797
            K + A ++ +  ++  DL       +SS    +    + D    AIV   +L+ D ARG+FFPT+W  +  LGG  +ALGY V +FS GR L+SP+FG  S  HGY + LL S  +++ G   YT A +V    F +F +QV +G+GSGTLGVTR +VAD T    RT ++A+ TAVQY+GFTV P +G  F+ +L   E  + L RF    QFTAPA+F+  + TA  VL+   F++ +R      P ++ + S  A  +T+              EDA NQ                              ++ VG  V                                                                                   D  I G  LLNVSTKG+I+ FET+G   A ++F ++S+ AG + ATCG+IGV ALLSM  + +  +DIQL+ GGM +M    + L F     +     F  A+FLIYSVGYPIGHTAV+GLFSK+VG +PQG L+GWF SAG++AR  FP+++G L+   G +++F  L+ +L
Sbjct:   34 KDERAGAVNIDQDDVDDLQSLAPSIASSVLTAD---GIHDPIGFAIVCLVILVGDMARGVFFPTMWPLVEALGGSSVALGYSVASFSFGRILVSPLFGGWSVTHGYSKTLLFSCSILWVGTLLYTQAQNVGHVGFLIF-AQVVMGLGSGTLGVTRAFVADVTAQRSRTTYMAWITAVQYSGFTVTPFVGSFFTKVLNDKEYKIGLFRF---NQFTAPAYFMNLVITATLVLMLQFFRNRVRISA---PKNNNKKS--AKRMTI--------------EDAANQ------------------------------VTFVGLTVY----------------------------------------------------------------------------------DCCILGCMLLNVSTKGSISAFETLGVSIAQSHFDMSSSRAGVIVATCGSIGVVALLSMGHMARFLSDIQLICGGMGIMCFGVLSLGFLEKDVINPSWKFCTAIFLIYSVGYPIGHTAVIGLFSKIVGRRPQGALLGWFASAGSLARLCFPIMSGYLTNFFGVSSVFYVLLCVL 472          

BLAST of mRNA_P-lacustris_contig1015.236.1 vs. uniprot
Match: A0A448Z9T0_9STRA (MFS domain-containing protein n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z9T0_9STRA)

HSP 1 Score: 268 bits (686), Expect = 4.080e-79
Identity = 198/550 (36.00%), Postives = 271/550 (49.27%), Query Frame = 1

Query:  199 LDDKASLAIVLGDVLLVDTARGIFFPTLWAHINQLGGDKIALGYCVGAFSLGRTLMSPVFGRMSTQHGYRRALLISTFLVYFGAFFYTVADSV----FSVFLSQVFLGVGSGTLGVTRGYVADKTTTEQRTYFLAYATAVQYAGFTVMPVLGGLFSYLLGSDEIPL-LGRFLTLTQFTAPAFFLMALSTAVFVLVYFVFQDGIRTQPAQRPSSSKELSGAASGVTLATDIGSDVDISSADEDALNQGQTGQWPASEYFPLDSISSSGSLPRRGSSTLSVVGHYVEISNDSYRSGSDDGKSAEDVEGGYQTGLAAVAADSGRVYVSINDSEEYEARDGEVRKETSKTLGEGVGAWLARKLRVHLPSRGDMLIYGGFLLNVSTKGTIACFETIGAEYAMTNFSLTSAEAGSMFATCGAIGVAALLSMRLLCKRFNDIQLVLGGMSLMILTCVILAF------PPSLTGLHVFVVAVFLIYSVGYPIGHTAVLGLFSKVVGAQPQGTLMGWFGSAGAMARTFFPVLTGVLSQELGSAALFLFLVGILSVTFVL 1815
            + D    AIV   +L+ D ARG+FFPT+W  + +LGG  +ALGY V +FS GR L+SP FG  S  HGY + LL S  +++ G   YT A +V    F +F SQ+ LG+GSGTLGVTR +VAD T    RT ++A+ TAVQY GFTV P +G  F+ +L   E    L RF    Q+TAPA+F+  + T   +L+   F+D +R    +   ++K+ S  A  +T+              EDA N+                                     V +                       TGL         VY                                            D  I G  LLNVSTKG+I+ FET+G   A + F + S++AG + A+CG+IGV ALLSM    +   DIQL+ GGM++M    + L F       PS      F  A+FLIYSVGYPIGHTAV+GLFSK+VG +PQG L+GWF SAG++AR  FP++ G L+   G +A+F  L+ +LSV+ ++
Sbjct:   66 IHDPVGFAIVCLVILVGDMARGVFFPTMWPLVEELGGSTVALGYSVASFSFGRILVSPKFGGWSVTHGYSKTLLFSCSILWVGTILYTQAQNVGHVGFLIF-SQIVLGLGSGTLGVTRAFVADVTAQRSRTTYMAWITAVQYGGFTVTPFVGSFFTKVLKDKEYRFGLFRF---NQYTAPAYFMNLVITVTIILMLKYFRDRVRMSALK---NNKKKS--AKRMTI--------------EDAANR-------------------------------------VTV-----------------------TGLT--------VY--------------------------------------------DCCILGCMLLNVSTKGSISAFETLGVSIAQSQFDMPSSQAGVIVASCGSIGVIALLSMGQXARFLTDIQLICGGMAIMCCGVLSLGFLEADVTNPSWK----FCTAIFLIYSVGYPIGHTAVIGLFSKIVGRRPQGELLGWFASAGSLARLCFPIMAGYLTNFFGISAIFFVLLIVLSVSIII 476