mRNA_P-lacustris_contig1087.721.1 (mRNA) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1090 bits (2819), Expect = 0.000e+0
Identity = 564/817 (69.03%), Postives = 649/817 (79.44%), Query Frame = 2

Query:  728 PAWASGASKFSPPSLYLVENALLTTAKDDFNGHAEAFMAAVGWNGQKLRRSTEDSRAPHMACTAYEQGHESFTRLQLLLSPEAVRIVSNSQDHGACFFVTASDVEAGAIAQDQEKFGLESFSPFPSALKLAQGLLDHGGSDETE-EASNNPGRLSARHGIKMRMANVQGLNVELTPGTLPAHSPEAEYFISDLIGDLMSESLDLHATNFWSDPAILEGEHLAYPEGAVRARDWSKAATVVHELSKAAGTGPGDICSWDSVVVLNGGGDSLSVSGLDHLLFSGRGVGGSHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFIDDDGEKIDHGYYFDELGYAAYYYAGAPTSVFTPPSQFTGGDFTYYPERRKIIQYIDLVNPDSLDSTY-ISQGYFGYFLPADEFGEDEEARGGAGHGTHTAGSAAGATLTTPAEMPTCSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYPDLDRMCPMHGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETVRPKCTINTPAIGKNALAVGASTSGDTRIISSVGATGIDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGGDEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSDGFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEG 3172
            PA A+ A  +SPPSL LVEN L TTAKDDFNGHA AF AA  WNG++LRRS + +RAPH+ C  Y  G E+F+RL+  LSP+AVR+VS+S+DHGACF  TAS  +A AI +DQE+FGLESFSPFPSALKLA GL+DH  S E E E S+   RL ARHG  MRM+NV+GLNV+LTP TLPA S  AE FI DL+GDLMS S+DLH+TNFWSDP +  GEHL+   GAVRARDWSKAA+VVHELS+AAGT PGDICSWD V ++  G DSL V+GLDHLLFSGRG GGSHEEE+TELHVACFMGLVSF A R EVLRVSP H+KR+LN AAR +IQSA++TDTPLTDAGLDGTGEVIQ++DTGLDETSC+FIDD GE++DHGY+F+EL   +    G+   ++ P   F  GDFTY  ERRK+IQYI+LVNPDS    +  +QG    FLP DEFG+D+ A    GHGTHTAGSAAGATL  PAE  TCSG E LGCVG C+D+S          TDDLV S ++Q+A PDLDR+CPM+GCD STD  CLSD+V +TLT NGGMAQGAKL+IFD FF    +   +G G +E C++AGCK+HS SLGGD LC+    ++L D FMY+NPENL+I AAGNDGET R  CT+ +PAIGKN LAVGASTSGDTR+IS+ G  GID VA FSSYGFT DGRIKPEVVAPGDMVYSAASDG D HSCRLWAYQGTSMSCPIVAGA+AM+RQYFVD  FY ADVTARGFCSDGF+CEGFSPS+ATVKALLINSANLMG SS+PD  RGFGRIHLE GMPL GEG
Sbjct:   20 PARAAAADTWSPPSLELVENGLATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGS---TGSSQLIYYPV--FEAGDFTYTEERRKVIQYIELVNPDSDYVAFSTTQGGVYPFLPPDEFGQDDSA----GHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESET--------TDDLV-SYAIQIALPDLDRLCPMYGCDGSTDP-CLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVISTEGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEG 817          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 1053 bits (2724), Expect = 0.000e+0
Identity = 553/819 (67.52%), Postives = 633/819 (77.29%), Query Frame = 2

Query:  737 ASGASKFSPPSLYLVENALLTTAKDDFNGHAEAFMAAVGWNGQKLRRSTEDSRAPHMACTAYEQGHESFTRLQLLLSPEAVRIVSNSQDHGACFFVTASDVEAGAIAQDQEKFGLESFSPFPSALKLAQGLLDHGGS-DETEEASNNPG--RLSARHGIKMRMANVQGLNVELTPGTLPAHSPEAEYFISDLIGDLMSESLDLHATNFWSDPAIL-EGEHLAYPEGAVRARDWSKAATVVHELSKAAGTGPGDICSWDSVVVLNGGGDSLSVSGLDHLLFSGRGVGGSHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFIDDDGEKIDHGYYFDELGYAAYY---YAGAPTSVFTPPSQFTGGDFTYYPERRKIIQYIDLVNPDSLDSTYISQGYFGYFLPADEFGEDEEARGGAGHGTHTAGSAAGATLTTPAEMPTCSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYPDLDRMCPMHGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETVRPKCTINTPAIGKNALAVGASTSGDTRIISSVGATGIDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGGDEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSDGFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEG 3172
            A  A + + P+L LVENALLTTAKDDF GH  AF AA  WNG++LR S E++RAPH+AC  Y  GHE+ +RL+  LSPEAVR+VS+S+DHGACFF +AS  +A AI +DQE+FGLE+FSPFPS LKLA GL+DH  S DE EE + + G  RLSARHG +MR  NV+GL+VELTPGTL A S EA+ FI+ ++GDLMS S+DLH+TNFWSDP +   GEHL+ P GAVRARDW KAA+VVHELS+AA TGPGDICSW+ + V + G DSL VSGLDHLLFSGRG GGSHEEE+TELHVACFMGLVSFLA R EVLRVSP  +KR+LNAAAR VIQSA++TDTPLTDAGLDGTGE+IQ+ID+GLDETSCYFIDD GE++DHGYYFDELG AA Y   Y+   T+       F GGDF+YYPERRK                                         AGHGTHTAGSAAGATL  PAE  TC+ T+ L CVGAC+D            TDDLV S+S Q    DLDR+CPM+GCD+STDTTCLSDDV +TL++NGGMAQGAKLAIFD F+     S+S+G   +E C +AGCKLHSNSLG D  CSI   D+LNDQFMY+N ENL+I AAGN+G   R  CTINTP IGKN+LA+GASTSGDTR+IS+VGATGIDTVA FSS+GFT D RIKPEVVAPGD VYSAASDG D HSCRLWAY+GTSMSCPIVAGA+AMIRQYFVD  FY ADVTARGFCSDGF+CEGFSPS+ATVKALLINSANLMGGSSEPDG RGFGR+HLEAGMPL GEG
Sbjct:   18 AVAAGRLNSPALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSSYSSDETATI-----FNGGDFSYYPERRK-----------------------------------------AGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACID--------LLDATDDLV-SISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEG 781          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 943 bits (2438), Expect = 0.000e+0
Identity = 515/838 (61.46%), Postives = 612/838 (73.03%), Query Frame = 2

Query:  764 PSLYLVENALLTTAKDDFNGHAEAFMAAVGWNGQKLRRSTEDSRAPHMACTAYEQGHESFTRLQLLLSPEAVRIVSNSQDHGACFFVTASDVEAGAIAQDQEKFGLESFSPFPSALKLAQGLLDHGGS-DETEEASNNPGRLSARHGIKMRMANVQGLNVELTPGTLPAHSPEAEYFISDLIGDLMSESLDLHATNFWSDPAILEGEHLAYPEGAVRARDWSKAATVVHELSKAAGTGPGDICSWDSVVVLNGGGDSLSVSGLDHLLFSGRGVGG-SHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFI-DDDGEKIDHGYYFDELGYAAYYYAGA---PTS-------------------VFTPPSQFTGGDFTYYPERRKIIQYIDLVNPDSL-----DSTYISQGYFGYFLPADEFGEDEEARGGAGHGTHTAGSAAGATLTTPAEMPTCSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYPDLDRMCPM---HGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETVRPKCTINTPAIGKNALAVGASTSGDTRIISSVGATGIDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGG-DEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSDGFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEGT 3175
            PSL LVENAL TTAKDDFNGHA AF AA  WNG++LR   +++RAPH+ C  Y  G E+F+RL+  LSPEAVR+VS+S+D GACFF +AS  +A AI +DQE+FGLESFSPFPSALKLA GL+DH    D+ +E +    RLSARHG +MRM+NV+GL++ELTPGTL + S EAE FISD +GDLM  S+DLH+TNFWSD  +  GE L++P GAVRARDWSKAA+VVHELS+AAGT PGDICSW+ V V +  GD L VSGLDHLL SGRG GG S   E+ ELH+ACFMGLVSFLAS+ EVLRV+P  S+ LLNA+ARA IQSAT TDTPLTDAGLDGTG+V+Q+ID+G+DE+SC+F  D+ G+ + HG+YF+E G      + +   PTS                    +    +F GGDFT +P+RRKIIQYI+L+  D       DS   S G    +LPAD F  D EA    GHGTH AGS AGATL TPAE+ +C  TE + CVGACVDD            DDL T     +   DLDR+CP+   + CD      CLSDDVA+TLT+NGGMAQGAKLAIFD F+ +  +S  VG G +E C +A CK+HSNS GGD  CS+ P DVL D +M  NPENL+I AAGNDGE  R  CT+++PAI KN LAVGASTSGDTR+ ++ G  GIDTVA FSSYG T D RIKPEV+APGD +YSAASDG  D HSCRLWAY GTSMS PIVAGASAMIRQYF++  F+A D+TARGFC +   CE FSPSSAT+KAL+INSANLMGGSSEPDG RGFGRIHLEAGMPL GEG+
Sbjct:   22 PSLELVENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEA----GHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEAND------VDDLATP---NVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGL-GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGS 845          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 858 bits (2216), Expect = 1.300e-286
Identity = 479/837 (57.23%), Postives = 569/837 (67.98%), Query Frame = 2

Query:  713 LGAAVPAWASGASKFSPPSLYLVENALLTTAKDDFNGHAEAFMAAVGWNGQKLRRSTEDS-RAPHMACTAYEQGHESFTRLQLLLSPEAVRIVSNSQDHGACFFVTASDVEAGAIAQDQEKFGLESFSPFPSALKLAQGLLDHGGSDETEEASNNPGRLSARHGIKMRMANVQGLNVELTPGTLPAHSPEAEYFISDLIGDLMSESLDLHATNFWSDPAILEGEHLAYPEGAVRARDWSKAATVVHELSKAAGTGPGDICSWDSVVVLNGGGDSLSVSGLDHLLFSGRGVGGSHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFIDDDGEKIDHGYYFDELGYAAYYYAGAPTSVFTPPSQFTGGDFTYYPERRKIIQYIDLVNPDSLDSTYISQGYFGYFLPADEFGEDEEARGGAGHGTHTAGSAAGATLTTPAEMPT-CSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYP-DLDRMCPMHGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETV--RPKCTINTPAIGKNALAVGASTSGDTRIISSV----------GATGIDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGGDEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSD-GFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEGT 3175
            L AA   W   A+    PSL +VE +LLTTA+D F+GHA+AF  A  W G+ LR   ED+ RAPH+ CT Y +G ++  RLQ LLSP AVR V++S  HGACFFVTAS  EA A++ D   +GL S SPFPSALK+A G+L+HG S   ++AS +PGRL+  HG  MR  NV+GLN+EL+PGTLPAHSPEA  FI++L+ DLMS+S+DLH  N WSDP    GEHL  P GAVR R+WS AAT+VH+L+ A  T PGDICSWDS+ +   G D L VSGLDHLL    G  GS +EEATEL VACFMGLVSFL++R EVLRVSP H+ RLLNA AR +IQSAT T+ PLTDAGLDGTG+VIQ+IDTGLDETSC+FIDDDGE++ HG+Y++ LG                                                           +P+     D+      GHGTH AGSA GAT+ TPAE PT CSGTE  GCVG C+D    A R    + DDL T +     Y  D+DR+CPM  CDD+TD  CLSDDV QTL D+GGMAQGAKLA FD F    + +  VG G +E C++AGCK+HS S+G D  CS+  +DVL D FMY+NPENL++VAAGN G+T   R  CT+ +PAIGKNALAVGA++SG+TRI  +           G   +DTVA FSSYG T DGRIKPEVVAPGD +YSA SDG D HSCRL A  GTSM+CPIVAGASAM+RQYF+D +FY AD  ARG+  D G  CE FSPSSATVKALLINSANLMGGSSEPDG RGFGRIHLE GMPL GEG+
Sbjct:   15 LAAASTPWGGSANTLPYPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMS-DSPYYGLTSISPFPSALKVAPGVLEHGVS--LDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGS-DEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALG---------------------------------------------------------EIIPSSSSARDDVE----GHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCID----ADR--TSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGS 780          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 803 bits (2074), Expect = 1.100e-264
Identity = 474/895 (52.96%), Postives = 575/895 (64.25%), Query Frame = 2

Query:  680 TCTSRFTRGLLLGAAVPAWASGASKFSPPSLYLVENALLTTAKDDFNGHAEAFMAAVGWNGQKLRRSTEDS-RAPHMACTAYEQGHESFTRLQLLLSPEAVRIVSNSQDHGACFFVTASDVEAGAIAQDQEKFGLESFSPFPSALKLAQGLLDHGGSDETEEASNNPGRLSARHGIKMRMANVQGLNVELTPGTLPAHSPEAEYFISDLIGDLMSESLDLHATNFWSDPAILEGEHLAYPEGAVRARDWSKAATVVHELSKAAGTGPGDICSWDSVVVLNGGGDSLSVSGLDHLLFSGRGVGGSHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFIDDDGEKIDHGYYFDELGYAAYYYAGAPTSVFTPPSQFTGGDFTYYPERRKIIQYIDLVNPDSLD---STYISQGYFGYFLPADEF-GEDEEARGGAGHGTHTAGSAAGATLTTPAEMPT-CSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAY-PDLDRMCPMHGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETV--RPKCTINTPAIGKNALAVGASTSGDTRI-ISSV---------GATGIDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGGDEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSD-GFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEGTW--------FFSWPTLSTHPSPGIALMNTCSTLMPTLAWICVP 3280
            T T+ F   L L AA   W   A+    PSL +VE +LLTTA + F+GHA+ F  A  W G  +R   ED+ RAPH+ CT Y +G E+ +RLQ LLSPEAVR V++S  HGACFFVTAS  EA A++ D   +GL S SPFPSALK+A G+L+HG S   ++AS +PGRL+  HG  MR  NV+GLN+EL+PGTLPAHSP+A  FI++L+ DLMS+S+DLH  N WSDP    GEHL  P GAVR R+WS AAT+VH+L+ A  T PGDICSWDS+ + + G D L VSGLDHLL    G  GS +EEATEL VACFMGLVSFL+SR EV RVSP H+ RLLNA AR +IQSAT T  PLT AGLDGTG+VIQ+I                                                                QYI+L+  DS+    S+  S G   +++  D F G+D +      HGTHTAGSA GAT+ TPAE PT CSGTE  GCVG C+D    A R    + DDL+T +    +Y  D+DR+CPM GCD+  +  CLSD V QTL D+GGMAQGAKL+ FD +  ++     +G G +E C++AGCK+HS S G D  C++   D+L D FMY+NPENL++VAAGN GE    R  CT+ +PAIGKNALAVGA++SG+TR+ ++S          G + +DTVA FSSYG T DGRIKPEVVAPGD VYSA SDG D HSCRL A  GTSM+CPIVAGASAM+RQYF+D +FY AD  ARG+C D G  CE FSPSSATVKALLINSANLMGGSSEPDG RGFGRIHLE GMPL GEG+         + S P LS+         N       TL+WI  P
Sbjct:    7 TATALF---LSLVAASTPWGGSANSLPYPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMS-DNPYYGLTSISPFPSALKVAPGVLEHGVS--RDQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGS-DEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI----------------------------------------------------------------QYINLIKADSVSGPASSTTSGGNRVWWVSPDGFQGDDVDD-----HGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCID----ADR--TSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPP 819          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 747 bits (1929), Expect = 2.180e-246
Identity = 393/556 (70.68%), Postives = 444/556 (79.86%), Query Frame = 2

Query: 1532 LSVSGLDHLLFSGRGVGGSHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFIDDDGEKIDHGYYFDELGYAAYY---YAGAPTSVFTPPSQFTGGDFTYYPERRKIIQYIDLVNPDS-LDSTYISQG-YFGYFLPADEFGEDEEARGGAGHG----THTAGSAAGATLTTPAEMPTCSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYPDLDRMCPMHGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETVRPKCTINTPAIGKNALAVGASTSGDTRIISSVGATGIDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGGDEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSDGFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEG 3172
            +  +GLDHLLFSGRG GGSHEEE+TELHVACFMGLVS LA R EVLRVSP  +KR+LNAAAR ++QSA++TDTPLTDAGLDGTGE+IQ+ID+GLDETSCYFIDD GE++DHGYYFDEL  AA Y   Y+   T+       F GGDF+YYPERRKIIQYIDLVNPDS   S Y SQG Y GYFLP D+FGED+EAR               SAAGATL  PAE  TC+ T+ L CVGAC+D            TDDLV S+S Q    DLDR+CPM+GCD+STDTTCLSDDV +TL++NGGMAQGAKLAIFD F+ D   S+S+G   +E C +AGCKLHSNSLG D  CSI   D+LNDQFMY+N E+L+I AAGN+G   R  CTINTP IGKN+LA+GASTSGDTR+IS+VGATGI+TVA FSS+GFT D RIKPEVVAPGD VYSAASDG D HSCRLWAY+GTSMSCPIVAGA+AMIRQYF+D  FY ADVTARGFCSD F+CEGFSPS+ATVKALLINSANLMGGSSEPDG RGFGR+HLEAGMPL GEG
Sbjct:    1 MHATGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVYSSLYSSDETATI-----FNGGDFSYYPERRKIIQYIDLVNPDSPYSSFYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACID--------LLDVTDDLV-SISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEG 542          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 713 bits (1841), Expect = 1.500e-229
Identity = 409/849 (48.17%), Postives = 544/849 (64.08%), Query Frame = 2

Query:  701 RGLLLGAAVPAWASGASKFSPPSLYLVENALLTTAKDDFNGHAEAFMAAVGWN-GQKLRRSTEDSRAPHMACTAYEQGHESFTRLQLLLSPEAVRIVSNSQDHGACFFVTASDVEAGAIAQDQEKFGLESFSPFPSALKLAQGLLDHGGSD--------ETEEASNNPGRLSARHGIKMRMANVQGLNVELTPGTLPAHS---PEAEYFISDLIGDLMSESLDLHATNFWSDPAILEGEHLAYPEGAVRARDWSKAATVVHELSKAAGTGPGDICSWDSVVVLNGGGDSLSVSGLDHLLFSGRGVGGSHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFIDDDGEKIDHGYYFDELGYAAYYYAGAPTSVFTPPSQFTGGDFTYYPERRKIIQYIDLVNPDSLDSTYISQGYFGYFLPADEFGEDEEARGGAGHGTHTAGSAAGATLTTPAEMPTCS-GTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYPDLDRMCPMHGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETV-RPKCTINTPAIGKNALAVGASTSGDTRIISSVGATG-----------IDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGGDEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSDGFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEG 3172
            RG      + +  SGAS+   PSL   E  LLTTA  DF+  A AF  A  W  G +  RS+     P+++C  + +G  ++ +L+  L   AV+ VS++  HGACF VTAS  EA  ++  ++     +F  FP+ALK+A GLLDHG  D         T  +  + GRLS  HG  M++ +  GL VEL+PGTLP+HS    +A  FI +L+  L S SLDLHA   WSD  +  G+HL    GA+R R+WS+AAT+V+E S++    P  +C W+ VVV +   D L V+GLDHLL+ GR V G+ EEEA EL +AC+MGLVSFLASR EVL+V+  + K  LNAAARA IQSA+   TPLTDAGLDG+GEVIQIID G++ET+C+    DG +++HGYYFD +  +   +  +  S     ++FTGGDF+Y  +RRK+IQYIDL+NPD+ D +  S G    F+ AD F  D++     GHGTH A + AG+T+++PAE+ +C+ G+E +GC G C+ +S   G     Y +D+             +R+CP  GC   +   CL DD A TL+++GGMAQGAK+A  D F+ D +     G G +E C DAGCK+HSNS G D  C +   DVL D++MY+N E+L++ +AGN G T  R  CTI++P I KN L VG+++SG TR+ S+    G           ID V+ FS YGFT DGRIKPEVVAPGDM+YSA+ +GGD  SC L AY GTS S  + +GA+AM+RQYF D +FY+ADV +RGFC  GF C+ FSP + TVKA+LINSAN+MGG +EPD  RGFGRIHLEAGMPLGG G
Sbjct:    6 RGAATSVLLISTVSGASQV--PSLGFFEQTLLTTAGADFDSEA-AFAFASAWQAGDRQGRSS-----PYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWED---FTTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGS--SSGM--PFVSADSFVSDDDL----GHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESS-TGSSEADYGEDI-------------NRLCPAFGC---SGDQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRL-STTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAG 816          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 605 bits (1561), Expect = 2.850e-187
Identity = 418/951 (43.95%), Postives = 534/951 (56.15%), Query Frame = 2

Query:  731 AWASGASKFSP----PSLYLVENALLTTAKDDFNGHAEAFMAAVGWNGQKLRRSTEDSR-APHMACTAYEQGHE-SFTRLQLLLSPEAV--RIVSNSQDHGACFFVTASDVEAGAIAQDQEKFGLESFSPFPSALKLAQGLLDHGGSDETEEASNNPGRLSARHGIKMRM----------------------------------------------ANVQGLNVELTPGTLPAHSPEAE-----YFISDLIGD-----LMSESLDLHATNFWS--------DPAILEG--EHLAYPEGAVRARDWSKAATVVHELSKAAGTGP--GDICSWDSVVVLNGGGDSLSVSGLDHLLFSGRGVGGSHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFIDDDG-EKIDHGYYFDELGYAAYYYAGAPTSV---FTPPSQFTGGDFTYYPERRKIIQYIDLVNPDSLDSTYISQGY----------------------FGY-----------FLPADEFGEDEEARGGAGHGTHTAGSAAGATLTTPAEMPT------CSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYP-----DLDRMCPMHGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFD--GFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETVRPKCTINTPAIGKNALAVGASTSGDTRIISSVGATG-----------IDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGGDEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSDGFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEG 3172
            A+A G +  +P    P L ++  ALLTTA   F  H   F AAV W+  +L    +D+R AP++AC + +     S   L  +L   A+  R+V +S +HG C   TA+  +A +I Q Q+ F   S++ FPSALKLA GLLDH   D   +     GRL   HG ++R                                               + V GL VEL PG L A +         Y  S  + D     LMS+SLD+H  +FWS        D A  EG  +      G V AR+W++AA V+  L    G GP  GD+CSWD +VV++   D L++ G+DHLL  GRG GG+   EA EL VACFM L+SFLASR EVLRVSP  + +LLNAAA   +QSA+ T++P+TDAGLDGTGEVIQI+DTGLD  SC+F+D+DG E + HGYYF+E+G  A  +    TS          FTGGDF +   RRK +  +D  +P S DS  IS+ +                      +G            ++ A EF ED+      GHGTHTAGSAAGA +++P+   T      C    +L CVG C+D     G       DDL+T+   Q  +      DLDR+CP   CDD  +  CL DD A+TL  +GG+A+GA+LAIFD    +  V +  + G G +E  M+AG KL SNS G  A C  +  DVL D FMY N + L++ AAGNDG+     CT+ TPA+ KN LAVGASTSG  R+ S     G           ID +A FSS+G T+D RIKPEVVAPGDM+YSAA  G   ++CRLW Y GTSMS PIVAGA+A++RQYF +  FYA DV  RGFC D F CE F+PS+ATVKA+LINSANLMGGS+EPDGLRGFGR+HLEAGMPL GEG
Sbjct:   15 AFAGGGASAAPTLPHPPLSVIRGALLTTAGVTFR-HQGEFKAAVEWHEARL----DDARPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFS--SWAAFPSALKLAPGLLDHPHDDR--DGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEG-GPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGL-VVDAAHP-SCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAG----GHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGE------DDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMA-GNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDWSG-CTMGTPALAKNVLAVGASTSGPGRL-SFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFC-DSFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEG 939          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 577 bits (1486), Expect = 2.190e-181
Identity = 329/553 (59.49%), Postives = 388/553 (70.16%), Query Frame = 2

Query: 1628 MGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFI-DDDGEKIDHGYYFDELGYAAYYYAGAPTSVFTPP---------------------------SQFTGGDFTYYPERRKIIQYIDLVNPDSL-----DSTYISQGYFGYFLPADEFGEDEEARGGAGHGTHTAGSAAGATLTTPAEMPTCSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYPDLDRMCPM---HGCDDSTDTTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTSVGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDGETVRPKCTINTPAIGKNALAVGASTSGDTRIISSVGATGIDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGG-DEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFCSDGFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEGT 3175
            MGLVSFLAS+ EVLRV+P  S+ LLNAAARA IQSAT TDTPLTDAGLDGTG+V+Q+ID+G+DE+SC+F  D+ G+ + HG+YF+E G          +S F+ P                            +F GGDFT YP+RRKIIQYI+L+  D       DS   S G    +L AD F  D+EA    GHGTH AGS AGATL TPAE+ +C+ TE + CVGACVDD            DDL T   +++A  DLDR+CP+   + CD      CLSDDVA+TLT+NGGMAQGAKLAIFD F+    +S  VGTG +E C DA CK+HSNS GGD  CS+   DVL D++M  NPENL+I AAGNDGE  R  CTI +PAI KN LAVGASTSGDTR+ ++ G+ GIDTVA FSSYG T D RIKPEV+APGD +YSAASDG  D HSCRLWAY GTSMS PIVAGASAMIRQYF++  F+A D+TARGFC +   CE FSPSSAT+KAL+INSANLMG SSEP+G RGFGRIHLEAGMPLGGEG+
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINP----SLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEA----GHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEAND------VDDLATPY-VEIA--DLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGS-GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGS 535          

BLAST of mRNA_P-lacustris_contig1087.721.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 549 bits (1414), Expect = 8.680e-168
Identity = 375/961 (39.02%), Postives = 512/961 (53.28%), Query Frame = 2

Query:  797 TTAKDDFNGHAEAFMAAVGWNGQKLRR-STEDSRAPHMACTAYEQGHESFTRLQLLLSPEAVRIVSNSQDHGACFFVTASDVEAGAIAQDQEKFGLESFSPFPSALKLAQGLLDHG-------GSDETEEASNNPGRLSARHGIKMRMANVQGLNVELTPGTLPAHSPE--AEYFISDLIGDLMSESLDLHATNFWSDPAILEGEHLAYPEGAVRARDWSKAATVVHELSKAAGTGPGDICSWDSVVVLNGGGDSLSVSGLDHLLFSGRGVGGSHEEEATELHVACFMGLVSFLASRQEVLRVSPMHSKRLLNAAARAVIQSATSTDTPLTDAGLDGTGEVIQIIDTGLDETSCYFIDDDGEKIDHGYYFDELGYAAYYYAGAPTSVFTPPSQFTGGDFTYYPERRKIIQYIDLVNP------------------------DSLDSTYISQGYFGYFLPADEFGEDEEARGG-----------------------------------AGHGTHTAGSAAGA-TLTTPAEMPTCSGTEDLGCVGACVDDSVIAGRYYXXYTDDLVTSLSLQLAYPDLDRMCPMHGCDDSTD---TTCLSDDVAQTLTDNGGMAQGAKLAIFDGFFRDVAVSTS-VGTGHFEACMDAGCKLHSNSLGGDALCSIVPEDVLNDQFMYDNPENLIIVAAGNDG--ETV--RPKCTINTPAIGKNALAVGASTSGD---TR-------IISSVGAT--------------------------GIDTVATFSSYGFTIDGRIKPEVVAPGDMVYSAASDGGDEHSCRLWAYQGTSMSCPIVAGASAMIRQYFVDMAFYAADVTARGFC---SDGFLCEGFSPSSATVKALLINSANLMGGSSEPDGLRGFGRIHLEAGMPLGGEGTWFFSWPTLSTHPSPGIALMNTCST----LMPTLAWICVPRYLGLTTQPQQH 3316
            ++A DDF     A+ AA  W   +L   + E S AP++AC  Y+QG  + T L+   +  AV  VSN++  G CF VTA    A A+ +  E F L S +P   +LKLA  LLDHG       G+ +  + S++   L + +G  M + NV GL+V L+PG L A   E  AE F+ D   DL SES+++   +FWSD      E+        R R+WS+AA VV  L+  +    G+IC +  + + +   D + V G++HLL   R          +E   AC+MGL+S LA++ EVLRVSP+ S RL NA A A++QSAT+T+TPL DAGLDGTGEVIQ++D+GLDETSC+F DDDG +++HG+ FD +            +V T    ++   F +   RRKI+QYI L                           D  DS YIS   +     +D + ED    G                                     GHGT TAGSAAGA +  +  E   C G E  GC G C+  S +         D ++   +      DLD  CPM+ CD +TD   + CLSDD  + L  +GG+A GA++++FD  +    +  S  G   + + M+ G K+H+NS GG  LC +  ++ L D FMY+NPE+L+I AAGNDG  E +  R  CT+ +PA+GKN+LAVGA++SG    TR       +   +G T                          G+DTVA FSS G T+DGRIKP+VVAPGD V SA+SDG D HSC+L A  GTS SCP+VAGA+A++RQYF D +F+A DV +RG C   S    C  F+PS+ATVKA++INSA+LMGGSSEP+G+RGFGR+HLEAGMP+ G G    S    +        L+         L  TLAW+  P    L+ +  QH
Sbjct:   23 SSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKR--------FPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVR------VEDDGNVSTVLGNYS---FPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDV---------DAMLDDSTF-----DLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIADGEITELVTVDGDAGVELRATLAWLDPPT-TALSAKQLQH 946