prot_P-lacustris_contig1012.219.1 (polypeptide) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: D7G858_ECTSI (Leucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G858_ECTSI)

HSP 1 Score: 1494 bits (3869), Expect = 0.000e+0
Identity = 719/805 (89.32%), Postives = 758/805 (94.16%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEG-------------DVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDPAGDPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAEDIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTLEKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 792
            MFPYPSG+GLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAI TGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTD +YVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQW LKITKYAD+LEAGL+GL+WP+GTLTAQKQWIGRSEGASITFDLEGT E++EVFTTRPDTLMGVTYLVLAPEHPLTL++AT ERR AV +YV ASASRSD+DRTSMGKGKKKTGTDTGA+AVHPIT +KVPVWTADYVLGSYGTGAVMAVPAHDERDLAFAR F+LPVRRVVKP+ GK                     +V+EAF G+GVAINSGEGFDGLSTEECAEAVVSQLE++QHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDP GDPR GD+HKICY+QP+PVDVSELPLTLPEM SFEPGDDPQGCLTR PDWRFFQRDG+WFARETNTMPQWAGSCWYYLRFTDTKN +AGWSKKAD+DWMPVDLYVGGQEHAVLHLLYARFWH+VLHDLGHVKDPEPFQ LVHQGMILGTDGEKMSKSRGNVINPDDIVD+ GADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQL+RRGAED+ELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMT EKPPREALEALV MVSPFAPHLGEECW LLG++KG+G++F PWV+WKEELCVSDSV LGVQVNGKVR ELELSKTASSD+ARALAE LPQVVKFTEGKEVKKFIYVPGRI+SFVVK
Sbjct:   55 MFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDKDYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWALKITKYADQLEAGLEGLNWPDGTLTAQKQWIGRSEGASITFDLEGTGESMEVFTTRPDTLMGVTYLVLAPEHPLTLEVATQERRAAVEEYVTASASRSDLDRTSMGKGKKKTGTDTGALAVHPITGEKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARTFELPVRRVVKPSGGKXXXXXXXXXXXXXXXXXXXXXEVVEAFTGRGVAINSGEGFDGLSTEECAEAVVSQLESQQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDPEGDPRRGDDHKICYDQPMPVDVSELPLTLPEMESFEPGDDPQGCLTRAPDWRFFQRDGKWFARETNTMPQWAGSCWYYLRFTDTKNADAGWSKKADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGTDGEKMSKSRGNVINPDDIVDSDGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAEDVELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTFEKPPREALEALVRMVSPFAPHLGEECWDLLGNNKGNGIAFAPWVEWKEELCVSDSVTLGVQVNGKVRTELELSKTASSDDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 859          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S4DRP5_9EUKA (Leucine--tRNA ligase n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S4DRP5_9EUKA)

HSP 1 Score: 1101 bits (2847), Expect = 0.000e+0
Identity = 530/808 (65.59%), Postives = 634/808 (78.47%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATD-ERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGN-----------KGAVEGDVLE--AFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDPAGDPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAEDIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTLEKP---PREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVV 791
            MFPYPSGAGLHVGHPEGYTASDVMARY+RMK +DVLHPMGWDSFGLPAEQHAI+TGTHP VTT  NI  F+RQL MLGFSYDWDRE+ATTD  +VKWTQWIFL+L+K GLA QSE  VNWCPALGTVLANEE+I+GKSERGDHPV R PLRQW LKIT+YADKL   L GL+WPEGTLTAQ+ WIGRSEGA I F+++G  E++ VFTTRPDTLMGVTY+ +APEHPL   +  D +  + V+ YV  ++S+SD+DRT+    K+KTG DTGA A+HPIT +++P+W ADYVLGSYG+GAVMAVPAHDERD AFA  F LP + VV+    K N           K  VE +  +  AF G+G  +NSGE FDGL  ++    ++ +LEAE  GR++I Y+LRDWIFSRQRYWGEPIPIYFPV M DP GDPR GDEH I Y+ PIPV   ELPL LPEM +F+PGDDPQGCL R  DWR+F +DG+W+ARETNTMPQWAGSCWYYLRF D  N +  +SK+AD+DWMPVDLYVGGQEHA LHLLYARFWH+VL++ G  K  EPFQ LVHQGMILGTDGEKMSKSRGNV+NPDD+V+ +GAD+LRLYEMFMGPLEATKPWQT QV+GV RFRD+VY L+ RG     +  +    +HK +KKV+ED++ ++FNTAISA+MV TN L    K    PR+A+E L L+VSPFAPHLGEECW +LGH+  D ++F PW ++ E LCV  +V +GVQVNGK+R +LE+ K A  D+A+  A A+P V +F  GKE+KKFIY PG+I++ VV
Sbjct:  116 MFPYPSGAGLHVGHPEGYTASDVMARYYRMKDYDVLHPMGWDSFGLPAEQHAINTGTHPAVTTLENIANFKRQLSMLGFSYDWDREIATTDIRFVKWTQWIFLQLYKQGLATQSEVSVNWCPALGTVLANEEVINGKSERGDHPVKRLPLRQWVLKITEYADKLIDDLDGLNWPEGTLTAQRTWIGRSEGAEIEFNIQGHDESLSVFTTRPDTLMGVTYVCIAPEHPLAKTLPIDADHLEKVSAYVEETSSKSDVDRTAAA-AKEKTGVDTGAKAIHPITGEEIPIWVADYVLGSYGSGAVMAVPAHDERDFAFADKFGLPRKEVVQAPPAKNNNXXXXXXXXXXKADVEDEKAQPSAFTGEGKLVNSGE-FDGLKGDKAKAKIIEKLEAEGKGRRKIQYKLRDWIFSRQRYWGEPIPIYFPVNMADPNGDPRKGDEHTIDYQTPIPVPEEELPLQLPEMDNFQPGDDPQGCLARAADWRYFLKDGKWYARETNTMPQWAGSCWYYLRFIDPLNDQQPFSKQADDDWMPVDLYVGGQEHATLHLLYARFWHKVLYEAGLTKTKEPFQKLVHQGMILGTDGEKMSKSRGNVVNPDDVVNEHGADSLRLYEMFMGPLEATKPWQTSQVSGVVRFRDKVYALATRGDLQDTMDEKIEKAMHKCIKKVTEDVESMSFNTAISAMMVFTNTLQDAAKEGMVPRKAVETLALLVSPFAPHLGEECWKMLGHE--DSLAFHPWPEYDEALCVESTVTIGVQVNGKLRGQLEIEKDAPEDKAKEAAMAIPGVEQFAGGKEMKKFIYRPGKIINIVV 919          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A6U3T6T3_9STRA (Leucine--tRNA ligase n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6U3T6T3_9STRA)

HSP 1 Score: 1093 bits (2826), Expect = 0.000e+0
Identity = 533/801 (66.54%), Postives = 634/801 (79.15%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSET-VEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAEDI---ELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTLE-KPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 792
            MFPYPSGAGLHVGHPEGYTASD+M+RYWRMKG+DVLHP+GWDSFGLPAEQ+AI+TG  P  TT  NID FRRQLKMLGFSYDW++E+ATTD  YVKWTQWIFL+LF  GLA+QS   VNWCPALGTVLANEE+I+G SERGDHPV RTPLRQWTLKIT YAD+LEAGL GLDWP GT+T+Q+QWIG+SEG +I F+++G  E  + VFTTR DTL GVTY+ LAPEHPL   I T E+++AV  YV  ++SRSD+DRT+    K+KTG  TG  AVHP+T +KVP+W  DYVLGSYGTGAVMAVPAHDERD  FA  +DL V+ VV+P   K  K   E +  EAF  +GV +NSGE FDGL+T +  +A+  +L+    G  +I Y+LRDW+FSRQRYWGEPIPIYFPVE    VDP+  DP++G EH+I Y+QPIPVD ++LPL LP M  F+PGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY RF D KN ++ +S K DE+WMPVDLYVGG EHAVLHLLYARFWHQVL DLG+ K PEPFQ LVHQGMILGTDGEKMSKSRGNVINPDDIV   GADALRLYEMFMGPLEA KPWQT QVAGV RF++++Y + +   ++    E+  ET  ILHKTMKKV+EDI+ L+FNTAISALMVLTNHL +L+   P+EA E L LMVSP APH+GEECW++LGHDK   +++  WV++ E LC+ ++V +GVQVNGKVR E+E++K A+ D          +VV   EGK+VKK IYVPGRI++ V K
Sbjct:  110 MFPYPSGAGLHVGHPEGYTASDIMSRYWRMKGYDVLHPIGWDSFGLPAEQYAINTGAQPVDTTKANIDNFRRQLKMLGFSYDWEKEIATTDVNYVKWTQWIFLQLFNKGLAKQSSVSVNWCPALGTVLANEEVINGLSERGDHPVERTPLRQWTLKITDYADRLEAGLDGLDWPSGTMTSQQQWIGKSEGCNIDFEIDGVDEEKITVFTTRADTLFGVTYVTLAPEHPLVSSITTAEQKEAVDAYVQKTSSRSDLDRTAA---KEKTGVFTGGYAVHPLTGEKVPIWVGDYVLGSYGTGAVMAVPAHDERDFEFAAKYDLDVKWVVEPQKNKKGK-KPELNTEEAFTVEGVNMNSGE-FDGLTTTKAKKAITYKLKEIGKGGPKITYKLRDWVFSRQRYWGEPIPIYFPVEFPEGVDPSTVDPKDGCEHEIKYDQPIPVDEADLPLELPLMTDFKPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPKNNDSAFSAKMDEEWMPVDLYVGGAEHAVLHLLYARFWHQVLFDLGYTKHPEPFQKLVHQGMILGTDGEKMSKSRGNVINPDDIVLGQGADALRLYEMFMGPLEAVKPWQTSQVAGVVRFQNKLYNVVKAAVKNENADEMNEETTKILHKTMKKVTEDIENLSFNTAISALMVLTNHLASLKGNVPKEAAEKLALMVSPIAPHIGEECWSMLGHDKS--LAYHEWVEYDEALCIDNTVTMGVQVNGKVRGEIEIAKDANQDXXXXXXXKQAKVVGQLEGKDVKKIIYVPGRILNIVAK 903          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: W7TSV9_9STRA (Leucine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TSV9_9STRA)

HSP 1 Score: 1088 bits (2815), Expect = 0.000e+0
Identity = 533/802 (66.46%), Postives = 622/802 (77.56%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSE---TVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVD-PAGDPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQR-DGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQL--SRRGAEDIE-LAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMT--LEKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 792
            MFPYPSG+GLHVGHPEGYTASDVMARYWRM   DVLHPMGWDSFGLPAEQHAI+TGTHP VTTY NI  FRRQLK LGFSYDW RELATTD +YV+WTQWIFL+L+K GLAEQSE  VNWCPALGTVLANEEIIDGKSERGDHPV R PLRQW LKITKYA++LE  LKGLDWPEGT+TAQK WIGRSEGA + F L  +     +VEVFTTRPDTLMGVTY+VLAPEHPL  ++ ++ERR AV  YV  +A +SD+DRT+ GK K KTG DTGA  VHP++ +++P+W ADYVL  YGTGAVMAVPAHDERD AFA  F LP+++VV P AG       E    EAF G+G  +NSG   DG  T E  + VV +LE  + G   +NYRLRDW+FSRQRYWGEPIPIYFPV+M D   GDPR  D H I ++QPIPV+ +ELPL LP+M +F PG+DPQGCL R  DWRFF++ DG+W+ARETNTMPQWAGSCWYYLRF+D  N +  W ++ADE W+PVDLYVGGQEHAVLHLLYARFWH+ L DLG  K  EPF  LVHQGMILGTDGEKMSKSRGNV+NPDD+V   G DALRLYEMFMGPLEA KPWQT QVAGV RFRD+V+QL  +R   +  E +  ETL ++HKT+KKV++D++ L+FNTAIS +M+  NHL +   E  PR A+E LVLMVSPFAPHLGEECW +LGHD+   ++  PW +++EEL V D V LGVQVNGK R E+ +   A+  +  A A    +V  F +GK V K IYVPGRI++ V K
Sbjct:  110 MFPYPSGSGLHVGHPEGYTASDVMARYWRMCDLDVLHPMGWDSFGLPAEQHAINTGTHPGVTTYENIANFRRQLKSLGFSYDWSRELATTDWDYVRWTQWIFLQLYKKGLAEQSEVLVNWCPALGTVLANEEIIDGKSERGDHPVERLPLRQWVLKITKYAEELEEDLKGLDWPEGTMTAQKLWIGRSEGARVMFPLACSGAIPGSVEVFTTRPDTLMGVTYVVLAPEHPLVERVTSEERRAAVRAYVREAAGKSDLDRTATGKDKGKTGVDTGAKVVHPLSGEELPIWVADYVLWGYGTGAVMAVPAHDERDHAFATIFGLPIKQVVAPDAGSDALPVEE----EAFVGEGQVVNSGPLLDGQPTSEAKKVVVEELEKNERGAAVVNYRLRDWVFSRQRYWGEPIPIYFPVDMADHDTGDPRKDDPHTIRFDQPIPVEEAELPLKLPDMDNFAPGNDPQGCLARAKDWRFFKKSDGKWYARETNTMPQWAGSCWYYLRFSDPTNLKRAWGEEADEQWLPVDLYVGGQEHAVLHLLYARFWHKFLFDLGLTKHREPFLKLVHQGMILGTDGEKMSKSRGNVVNPDDVVKEVGGDALRLYEMFMGPLEAVKPWQTAQVAGVVRFRDKVFQLVRNREMTDSTEAMDDETLRLMHKTIKKVTQDVETLSFNTAISTMMIFANHLASRKTETLPRAAVENLVLMVSPFAPHLGEECWEMLGHDQS--LACHPWPRYQEELTVDDVVKLGVQVNGKTRGEVSVPADATEADVMAAAMEQEKVASFLDGKRVVKVIYVPGRILNIVAK 905          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S4I9K5_9STRA (Leucine--tRNA ligase n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4I9K5_9STRA)

HSP 1 Score: 1087 bits (2810), Expect = 0.000e+0
Identity = 533/801 (66.54%), Postives = 623/801 (77.78%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTS-ETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGD-EHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAED--IELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 792
            MFPYPSGAGLHVGHPEGYTASDVM+RYWRM GFDVLHP+GWDSFGLPAEQ AI+TG  P  TT  NI  F+RQLKMLGFSYDWDRE+ATTD EYVKWTQWIFL+L+K GLAEQS   VNWCPALGTVLANEE+I+G SERGDHPV R PLRQW LKIT YAD+LEAGL GLDWP GTLTAQ+QWIG+S G +I FD++G   E +  FTTR DTLMGVTY+ LAPEHPL   ++T E++  V  YV A++SRSD+DRT+    K+KTG  TG  A+HPI+  KVP+W  DYVLGSYGTGAVMAVPAHD+RD  FA  ++L V+ VVKP  G  N         EAF   GVA NSGE FDG++T  C + V  +LE+   G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+    V+P   DP+  D EH I ++QPIPV+ ++LPL LPEM  F PGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY RF D KN +A +S K DEDWMPVDLYVGG EHAVLHLLYARFWHQVL DLG+ K  EPFQ LVHQGMILG+DGEKMSKSRGNV+NPDDIVD  GADALRLYEMFMGPLEA KPWQTEQV+GV RF+++ Y + +  AE+   ++  ET  ILHKTMKKV+EDID ++FNTAISA+MVLTNHL TL +K P EA E L LMVSP APHLGEECW +LG++  D +++  WV++ EELCV ++V +GVQVNGKVR E+E+ K A  D A   A    +V    EGK++KK I+VPGRI++ V K
Sbjct:  108 MFPYPSGAGLHVGHPEGYTASDVMSRYWRMNGFDVLHPIGWDSFGLPAEQFAINTGARPADTTAKNIANFKRQLKMLGFSYDWDREIATTDVEYVKWTQWIFLQLYKTGLAEQSSVSVNWCPALGTVLANEEVINGLSERGDHPVTRLPLRQWLLKITDYADRLEAGLDGLDWPSGTLTAQQQWIGKSVGCNIEFDVDGLEGEKITAFTTRADTLMGVTYVTLAPEHPLVSSVSTAEQKDEVDAYVKATSSRSDLDRTAA---KEKTGVFTGGYAIHPISGDKVPIWVGDYVLGSYGTGAVMAVPAHDQRDFEFATKYNLDVKWVVKPKKGDANTD-------EAFTANGVAANSGE-FDGMTTTTCKKEVTKKLESMDKGGAKITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVNPEDTDPKGDDCEHTIRFDQPIPVEEADLPLRLPEMDDFTPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPKNDDAAFSPKVDEDWMPVDLYVGGAEHAVLHLLYARFWHQVLFDLGYTKHSEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDDIVDRQGADALRLYEMFMGPLEAVKPWQTEQVSGVVRFQNKFYNVVKAAAENGCTDMDEETTKILHKTMKKVTEDIDSMSFNTAISAMMVLTNHLQTLKDKVPVEAAEKLALMVSPLAPHLGEECWNMLGNE--DSLAYHQWVEYDEELCVDNTVKMGVQVNGKVRGEIEIPKDADQDLAMEEANKQSKVASQLEGKDIKKIIFVPGRILNIVAK 895          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S2RFM0_9STRA (Leucine--tRNA ligase n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2RFM0_9STRA)

HSP 1 Score: 1086 bits (2808), Expect = 0.000e+0
Identity = 534/804 (66.42%), Postives = 626/804 (77.86%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSE--TVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMV-----DPAGDPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAE--DIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQ--VVKFTEGKEVKKFIYVPGRIVSFVVK 792
            MFPYPSG GLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ AI+TG  P  TT  NID FRRQLKMLGFSYDWDRE+ATTD  YVKWTQWIFL+L+K GLA+QS   VNWCPALGTVLANEE+I+G SERGDHPV R PLRQW LKIT YAD+LEAGL+GLDWP GT+ AQKQWIG+SEG +I FD+EG+ E   V VFTTR DTL GVTY+ LAPEHPL  KI T ++++ V  YV  ++SRSDMDRT+    K+KTG  TGA A+HP+T +KVP+W  DYVLGSYGTGAVMAVPAHDERD  FA  F L V+ VV+P      K   E    EAF   GVA NS + FDGL+T +  +A+   L+    G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+ +     D      +G EH I Y+QPIPVD SELPL LP M  F+PGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY R+ D KN E   SKK DE+WMPVDLY+GG EHAVLHLLYARFWHQVL DLG+ + PEPF+ LVHQGMILGTDGEKMSKSRGNV+NPDDIV+A GADALRLYEMFMGPLEA KPWQT QV+GV RF++++Y + ++  E  D+++  ET  ILHKTMKKV+EDI+ ++FNTAISA+MVLTNHL +L E  PREA E L LMVSP APHLGEECW+LLG+DK   +++ PWV++ E LCV ++V +GVQVNGK R E+E+ K A  D+  A++EA+ Q  V    EGK++KK IYVPGRI++ V K
Sbjct:  119 MFPYPSGTGLHVGHPEGYTASDVMARYWRMTGNDVLHPIGWDSFGLPAEQFAINTGAQPAETTTKNIDNFRRQLKMLGFSYDWDREIATTDVNYVKWTQWIFLQLYKKGLAQQSSVSVNWCPALGTVLANEEVINGLSERGDHPVERLPLRQWILKITDYADRLEAGLEGLDWPSGTMAAQKQWIGKSEGCNIDFDVEGSEEGDKVSVFTTRADTLFGVTYVTLAPEHPLISKITTADQKETVDDYVKKTSSRSDMDRTAS---KEKTGVFTGAYAIHPLTDEKVPIWVGDYVLGSYGTGAVMAVPAHDERDFEFASKFGLEVKWVVEP-----KKNPEEMSKEEAFTATGVATNSAD-FDGLTTAKTKKAITYNLKELNKGGPQITYKLRDWVFSRQRYWGEPIPIYFPVDFLEGVIPDDVDPKEDGCEHTIRYDQPIPVDESELPLELPHMEDFQPGDDPAGCLARAADWRYFQKDGKWFARETNTMPQWAGSCWYYFRYIDNKNDEEVISKKLDEEWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYTQHPEPFKKLVHQGMILGTDGEKMSKSRGNVVNPDDIVNAQGADALRLYEMFMGPLEAVKPWQTSQVSGVVRFQNKLYSVVKKAVENDDVKMDEETTKILHKTMKKVTEDIESMSFNTAISAMMVLTNHLQSLKENIPREAAEKLALMVSPIAPHLGEECWSLLGNDKS--LAYHPWVEYDESLCVDNTVTMGVQVNGKKRGEIEILKDA--DQETAMSEAMKQGRVESQLEGKDIKKIIYVPGRILNIVAK 909          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A1Z5K7R9_FISSO (Leucine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K7R9_FISSO)

HSP 1 Score: 1086 bits (2808), Expect = 0.000e+0
Identity = 528/800 (66.00%), Postives = 629/800 (78.62%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGT-SETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGD-EHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQL--SRRGAEDIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTLEKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 792
            MFPYPSGAGLHVGHPEGYTASDVMARYWRMKG+DVLHP+GWDSFGLPAEQ+AI TG  P  TT  NI  F+RQL+MLGFSYDWDRELATTD EYVKWTQWIFL+LF+ GLAEQSE  VNWCPALGTVLANEE+I+G SERGDHPV R PLRQW LKIT+YAD+LE GL+GL+WP GTL+AQKQWIGRSEG  I F +EG  +ET+ VFTTR DTLMGVTY+ LAPEHPL L + ++E++ AV+ YV  ++SRSD+DRTS    K+K+G  TG  A+HPI+ ++VP+W  DYVLGSYGTGAVMAVPAHD+RD  FA+ + L ++ VV+PA         E D   AF   GV +NSGE FDGL+TE+  EAV  +LE  + G  +INY+LRDW+FSRQRYWGEPIPIYFP++    VDP+  DP+  D +H I Y+QPIPVD ++LPL LP M  F+PGD+P GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY RF D KN++  +SKK DE+WMPVDLYVGG EHAVLHLLYARFWHQVL DLG+   PEPFQ LVHQGMILG+DGEKMSKSRGNV+NPDDIV + GADALR+YEMFMGPLEA KPWQT QVAGV RF ++ Y +  S    +  E+  ETL +++KTMKKV+EDI+ ++FNTAISALMV  NHL +LE  PREA + L LMVSPFAPHLGEECW +LGH   + +++ PWV + E LCV  +V +GVQVNGK R E+ ++K A    A A A  + +V    EGKE+KK IYVPGRI++ + K
Sbjct:   87 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGYDVLHPIGWDSFGLPAEQYAIQTGAQPADTTAANIANFKRQLQMLGFSYDWDRELATTDVEYVKWTQWIFLQLFRKGLAEQSEVSVNWCPALGTVLANEEVINGLSERGDHPVERLPLRQWVLKITQYADRLEEGLQGLEWPAGTLSAQKQWIGRSEGCRIDFGIEGMDTETITVFTTRADTLMGVTYVTLAPEHPLVLSVTSEEQKDAVSAYVNEASSRSDLDRTSS---KEKSGVFTGGYALHPISGERVPIWVGDYVLGSYGTGAVMAVPAHDQRDFEFAQKYGLDIKWVVEPAD------HAELDQTVAFTEYGVNVNSGE-FDGLTTEKAKEAVTKKLEELEKGGTQINYKLRDWVFSRQRYWGEPIPIYFPIDFPEGVDPSQQDPKLDDCQHTIRYDQPIPVDEADLPLKLPVMEDFQPGDNPAGCLARARDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPKNSKEAFSKKVDEEWMPVDLYVGGAEHAVLHLLYARFWHQVLFDLGYTTHPEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDDIVQSQGADALRMYEMFMGPLEAVKPWQTSQVAGVVRFMNKAYNIVVSAAATKHTEMGDETLRLMNKTMKKVTEDIENMSFNTAISALMVYANHLQSLETIPREAAKTLTLMVSPFAPHLGEECWNILGHT--ESLAYEPWVVFDEALCVDTTVTIGVQVNGKSRGEISIAKDADEATAVAAASGVDKVQVQLEGKEIKKIIYVPGRILNLIAK 874          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A448Z2U8_9STRA (Leucine--tRNA ligase n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z2U8_9STRA)

HSP 1 Score: 1083 bits (2802), Expect = 0.000e+0
Identity = 523/798 (65.54%), Postives = 626/798 (78.45%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGD-EHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAED--IELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFV 790
            MFPYPSGAGLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ A+ TGT P  TT  NI  FRRQLKMLGFSYDWD+ELATTDT YVKWTQWIFL+L+K GLAEQSE  VNWC ALGTVLANEE+I+G SERGDHPV R PLRQW LKIT+YAD+LE+GL GL+WP GT+T+Q+QWIG+S G  I FD+EG+ E+V+VFTTRPDTL+GVTY+ LAPEHPL   I T E++ +V KYV  ++SRSD+DRTS    K KTG  TGA   HPI+  +VPVW  DYVLGSYGTGAVMAVPAHD RD  FA+ FDL ++ VV+P  G+ NK        EAF   GVA+NSGE FDGL+T+ C +A+ ++LE    G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+    VDP   DP+N D EH I Y+QPIPVD ++LPL LP M +FEPGDDP GCL R  DWR+F++DG+WFARETNTMPQWAGSCWYY RF D  NTE  +S++ D+DWMPVDLY+GG EHAVLHLLYARFWHQVL DLG+ + PEPF+ LVHQGMILG+DGEKMSKSRGNV+NPDD+V   GADALRLYEMFMGPLEA KPWQTEQV GV RF++R+Y +    A+    ++  ET  ILHKTMKKV+EDI+ ++FNTAISA+MVLTNHL++L +K P EA E L LMVSP APHLGEECW++LGH   D +++  WV++ E LCV + V +G QVNGK+R ++ +   A  + A + A+ +  V    +GK ++K IYVPGRI++ +
Sbjct:  103 MFPYPSGAGLHVGHPEGYTASDVMARYWRMTGHDVLHPIGWDSFGLPAEQFAVQTGTQPAETTGKNIANFRRQLKMLGFSYDWDKELATTDTAYVKWTQWIFLQLYKKGLAEQSEVSVNWCAALGTVLANEEVINGLSERGDHPVQRLPLRQWVLKITEYADRLESGLDGLNWPSGTMTSQQQWIGKSVGCEIVFDVEGSDESVKVFTTRPDTLLGVTYVTLAPEHPLVSSITTPEQKDSVDKYVLETSSRSDLDRTSS---KVKTGVFTGAYVTHPISGDRVPVWVGDYVLGSYGTGAVMAVPAHDSRDFEFAKKFDLDMKWVVEPKKGELNKD-------EAFTEPGVAVNSGE-FDGLATKNCKKAITNKLEEINKGGAQITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVDPTSQDPKNDDCEHTIRYDQPIPVDEADLPLELPHMENFEPGDDPAGCLARAKDWRYFEKDGKWFARETNTMPQWAGSCWYYFRFLDPSNTEEAFSQQMDKDWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYSEHPEPFEKLVHQGMILGSDGEKMSKSRGNVVNPDDVVSEQGADALRLYEMFMGPLEAVKPWQTEQVPGVVRFQNRLYNVVNAAAKSKTTDMDEETARILHKTMKKVTEDIESMSFNTAISAMMVLTNHLVSLGDKVPLEAAENLALMVSPLAPHLGEECWSILGHK--DSLAYATWVEYDEALCVDNEVTIGCQVNGKMRGQITIPAEADEETAVSAAKEMENVQSQIDGKTIRKIIYVPGRILNII 887          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S2XSK2_9STRA (Leucine--tRNA ligase n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2XSK2_9STRA)

HSP 1 Score: 1078 bits (2787), Expect = 0.000e+0
Identity = 529/800 (66.12%), Postives = 629/800 (78.62%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGT-SETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPA-GDPR-NGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAED--IELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVV 791
            MFPYPSGAGLHVGHPEGYTASDVMARYWRMKG+DVLHP+GWDSFGLPAEQ AI+TGT P VTT +NI  F+RQLKMLGFSYDWDRELATTD E+VKWTQWIFL+LFK GLAEQS   VNWCP LGTVLANEE+I+G SERGD PV+R PLRQW LKIT YAD+LE GL+GLDWP GT+ AQKQWIG+S G++I F ++G  +E + VFTTR DTLMGVTY+ LAPEHPL L I ++E+++ V  YV  ++SRSD+DRT+    K+KTG  TG   +HPI+ +KVP+W  DYVLGSYGTGAVMAVPAHD RD  FA+ F L ++ VV+P+  K  K        EAF   G  INSGE FDGL+T +  +A+  +L     G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+    V+P+  DP+ +G EH I Y+QP PVD +ELPLTLPE+  F+PGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYYLR+ D  N+   +SK+AD DWMPVDLYVGG EHAVLHLLYARFWHQV+ DLG+ + PEPFQ LVHQGMILG+DGEKMSKSRGNV+NPD+IVDA GADALRLYEMFMGPLEA KPWQT QV+GV RF++++Y +     E+   E+  ET  ILHKTMKKVSEDID L FNTAISALMVLTNHL++L +K P EA E L LMVSPFAPHLGEECW LLGH   + +++ PWV++ E LC+   V +GVQVNGKVR E+ ++K A  D A + A    +V    +GK++KK IYVPGRI++F+V
Sbjct:  120 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGYDVLHPIGWDSFGLPAEQFAINTGTKPAVTTASNIANFKRQLKMLGFSYDWDRELATTDVEFVKWTQWIFLQLFKKGLAEQSSVSVNWCPELGTVLANEEVINGLSERGDFPVIRLPLRQWLLKITDYADRLEEGLEGLDWPSGTMAAQKQWIGKSIGSNIDFKVDGLDTEKISVFTTRADTLMGVTYVTLAPEHPLVLSICSEEQKEEVEAYVKETSSRSDLDRTAA---KEKTGVFTGGYGIHPISGEKVPIWVGDYVLGSYGTGAVMAVPAHDVRDFEFAQKFGLEIKWVVQPSQTKELK-----STEEAFTPHGACINSGE-FDGLTTAKAKKAITRKLAEMDMGGAKITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVNPSDADPKEDGCEHTIRYDQPFPVDEAELPLTLPEIDDFKPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYLRYLDPTNSGEAFSKQADADWMPVDLYVGGAEHAVLHLLYARFWHQVMFDLGYTQHPEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDEIVDAQGADALRLYEMFMGPLEAVKPWQTSQVSGVVRFQNKLYTVVNTAIENQVTEMDDETTRILHKTMKKVSEDIDTLGFNTAISALMVLTNHLVSLKDKVPLEAAEKLALMVSPFAPHLGEECWKLLGHT--EMLAYHPWVEYDESLCIDTDVTIGVQVNGKVRGEIFIAKDADQDLAMSEARKQERVAFQLDGKDIKKVIYVPGRILNFIV 908          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S4AJT1_9STRA (Leucine--tRNA ligase n=2 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4AJT1_9STRA)

HSP 1 Score: 1077 bits (2784), Expect = 0.000e+0
Identity = 523/799 (65.46%), Postives = 626/799 (78.35%), Query Frame = 0

Query:    1 MFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTS-ETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGD-EHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGA--EDIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFV 790
            MFPYPSGAGLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ+A+ TGT P  TT  NI  F RQLKMLGFSYDWD+ELATTDT YVKWTQWIFL+L+K GLAEQSE  VNWCPALGTVLANEE+I+G SERGDHPV R PLRQW LKIT+YAD+LEAGL GL+WP GT+T+Q+ WIG+S G  I FD+EG S ETV+VFTTR DTL+GVTY+ LAPEHPL   I T E+++ V  YVA ++SRSD+DRTS    K+KTG  TGA   HPI+  +VPVW  DYVLGSYGTGAVMAVPAHD RD  FA+ F+L V+ VV+P  G+ NK        EAF   GVA+NSGE FDGL+T+ C +A+ S+LE    G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+    VDPA  DP+N D EH I Y+Q IPVD ++LPL LP M +FEPGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY RF D  N +  +SK+ D+DWMPVDLY+GG EHAVLHLLYARFWHQVL DLG+ + PEPF+ LVHQGMILGTDGEKMSKSRGNV+NPDD+V   GADALR+YEMFMGPLEA KPWQT QV GV RF++R+Y +    A  +  E+  ET  ILHKTMKKV+EDI+ ++FNTAISA+MVLTNHL++L +K P EA E L LMVSP APHLGEECW +LG++  + +++ PWV++ E LCV D + +GVQVNGK+R ++ ++  A  + A + A+ +  V    +GK ++K IY+ GRI++ +
Sbjct:  103 MFPYPSGAGLHVGHPEGYTASDVMARYWRMTGHDVLHPIGWDSFGLPAEQYAVQTGTQPAATTKKNIANFMRQLKMLGFSYDWDKELATTDTNYVKWTQWIFLQLYKKGLAEQSEVSVNWCPALGTVLANEEVINGLSERGDHPVKRLPLRQWVLKITEYADRLEAGLDGLNWPSGTMTSQQAWIGKSIGCEIDFDVEGDSGETVKVFTTRADTLLGVTYVTLAPEHPLVSSITTPEQKEVVDSYVATTSSRSDLDRTSS---KEKTGVFTGAFVTHPISGDRVPVWVGDYVLGSYGTGAVMAVPAHDTRDFEFAKKFELDVKWVVEPKKGELNK-------EEAFTAPGVAVNSGE-FDGLATKNCKKAITSKLEEINKGGAQITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVDPASVDPKNDDCEHTILYDQAIPVDEADLPLELPYMENFEPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPNNNDEAFSKQMDKDWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYSEHPEPFEKLVHQGMILGTDGEKMSKSRGNVVNPDDVVGENGADALRMYEMFMGPLEAVKPWQTGQVPGVVRFQNRLYNVVNAAARSQTNEMDEETTRILHKTMKKVTEDIELMSFNTAISAMMVLTNHLVSLGDKVPLEAAEKLALMVSPLAPHLGEECWNILGNE--ESLAYAPWVEYDESLCVDDEITMGVQVNGKMRGKITIAADADEEAAVSAAKEMNTVQSQIDGKTIRKIIYIQGRILNII 888