mRNA_P-lacustris_contig1012.219.1 (mRNA) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: D7G858_ECTSI (Leucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G858_ECTSI)

HSP 1 Score: 1578 bits (4087), Expect = 0.000e+0
Identity = 763/860 (88.72%), Postives = 805/860 (93.60%), Query Frame = 1

Query:  325 GHVSTASEAAAVATVGSRLYDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEG-------------DVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDPAGDPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAEDIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTLEKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 2865
            G  S A EAAA   VGS+LY+HK IEARWQQHWEE+GTFKTPERTEGRPKKFVLDMFPYPSG+GLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAI TGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTD +YVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQW LKITKYAD+LEAGL+GL+WP+GTLTAQKQWIGRSEGASITFDLEGT E++EVFTTRPDTLMGVTYLVLAPEHPLTL++AT ERR AV +YV ASASRSD+DRTSMGKGKKKTGTDTGA+AVHPIT +KVPVWTADYVLGSYGTGAVMAVPAHDERDLAFAR F+LPVRRVVKP+ GK                     +V+EAF G+GVAINSGEGFDGLSTEECAEAVVSQLE++QHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDP GDPR GD+HKICY+QP+PVDVSELPLTLPEM SFEPGDDPQGCLTR PDWRFFQRDG+WFARETNTMPQWAGSCWYYLRFTDTKN +AGWSKKAD+DWMPVDLYVGGQEHAVLHLLYARFWH+VLHDLGHVKDPEPFQ LVHQGMILGTDGEKMSKSRGNVINPDDIVD+ GADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQL+RRGAED+ELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMT EKPPREALEALV MVSPFAPHLGEECW LLG++KG+G++F PWV+WKEELCVSDSV LGVQVNGKVR ELELSKTASSD+ARALAE LPQVVKFTEGKEVKKFIYVPGRI+SFVVK
Sbjct:    2 GMASAAPEAAAA--VGSKLYNHKAIEARWQQHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDKDYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWALKITKYADQLEAGLEGLNWPDGTLTAQKQWIGRSEGASITFDLEGTGESMEVFTTRPDTLMGVTYLVLAPEHPLTLEVATQERRAAVEEYVTASASRSDLDRTSMGKGKKKTGTDTGALAVHPITGEKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARTFELPVRRVVKPSGGKXXXXXXXXXXXXXXXXXXXXXEVVEAFTGRGVAINSGEGFDGLSTEECAEAVVSQLESQQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDPEGDPRRGDDHKICYDQPMPVDVSELPLTLPEMESFEPGDDPQGCLTRAPDWRFFQRDGKWFARETNTMPQWAGSCWYYLRFTDTKNADAGWSKKADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGTDGEKMSKSRGNVINPDDIVDSDGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAEDVELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTFEKPPREALEALVRMVSPFAPHLGEECWDLLGNNKGNGIAFAPWVEWKEELCVSDSVTLGVQVNGKVRTELELSKTASSDDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 859          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S4DRP5_9EUKA (Leucine--tRNA ligase n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S4DRP5_9EUKA)

HSP 1 Score: 1149 bits (2972), Expect = 0.000e+0
Identity = 557/855 (65.15%), Postives = 667/855 (78.01%), Query Frame = 1

Query:  349 AAAVATVGSRLYDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATD-ERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGN-----------KGAVEGDVLE--AFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDPAGDPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAEDIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTLEKP---PREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVV 2862
            AA VAT   R YD  EIE RWQ+ W E  TFKTPER +G+ K+++LDMFPYPSGAGLHVGHPEGYTASDVMARY+RMK +DVLHPMGWDSFGLPAEQHAI+TGTHP VTT  NI  F+RQL MLGFSYDWDRE+ATTD  +VKWTQWIFL+L+K GLA QSE  VNWCPALGTVLANEE+I+GKSERGDHPV R PLRQW LKIT+YADKL   L GL+WPEGTLTAQ+ WIGRSEGA I F+++G  E++ VFTTRPDTLMGVTY+ +APEHPL   +  D +  + V+ YV  ++S+SD+DRT+    K+KTG DTGA A+HPIT +++P+W ADYVLGSYG+GAVMAVPAHDERD AFA  F LP + VV+    K N           K  VE +  +  AF G+G  +NSGE FDGL  ++    ++ +LEAE  GR++I Y+LRDWIFSRQRYWGEPIPIYFPV M DP GDPR GDEH I Y+ PIPV   ELPL LPEM +F+PGDDPQGCL R  DWR+F +DG+W+ARETNTMPQWAGSCWYYLRF D  N +  +SK+AD+DWMPVDLYVGGQEHA LHLLYARFWH+VL++ G  K  EPFQ LVHQGMILGTDGEKMSKSRGNV+NPDD+V+ +GAD+LRLYEMFMGPLEATKPWQT QV+GV RFRD+VY L+ RG     +  +    +HK +KKV+ED++ ++FNTAISA+MV TN L    K    PR+A+E L L+VSPFAPHLGEECW +LGH+  D ++F PW ++ E LCV  +V +GVQVNGK+R +LE+ K A  D+A+  A A+P V +F  GKE+KKFIY PG+I++ VV
Sbjct:   70 AAGVATELKR-YDPAEIEPRWQKIWAEEKTFKTPERRDGKEKRYILDMFPYPSGAGLHVGHPEGYTASDVMARYYRMKDYDVLHPMGWDSFGLPAEQHAINTGTHPAVTTLENIANFKRQLSMLGFSYDWDREIATTDIRFVKWTQWIFLQLYKQGLATQSEVSVNWCPALGTVLANEEVINGKSERGDHPVKRLPLRQWVLKITEYADKLIDDLDGLNWPEGTLTAQRTWIGRSEGAEIEFNIQGHDESLSVFTTRPDTLMGVTYVCIAPEHPLAKTLPIDADHLEKVSAYVEETSSKSDVDRTAAA-AKEKTGVDTGAKAIHPITGEEIPIWVADYVLGSYGSGAVMAVPAHDERDFAFADKFGLPRKEVVQAPPAKNNNXXXXXXXXXXKADVEDEKAQPSAFTGEGKLVNSGE-FDGLKGDKAKAKIIEKLEAEGKGRRKIQYKLRDWIFSRQRYWGEPIPIYFPVNMADPNGDPRKGDEHTIDYQTPIPVPEEELPLQLPEMDNFQPGDDPQGCLARAADWRYFLKDGKWYARETNTMPQWAGSCWYYLRFIDPLNDQQPFSKQADDDWMPVDLYVGGQEHATLHLLYARFWHKVLYEAGLTKTKEPFQKLVHQGMILGTDGEKMSKSRGNVVNPDDVVNEHGADSLRLYEMFMGPLEATKPWQTSQVSGVVRFRDKVYALATRGDLQDTMDEKIEKAMHKCIKKVTEDVESMSFNTAISAMMVFTNTLQDAAKEGMVPRKAVETLALLVSPFAPHLGEECWKMLGHE--DSLAFHPWPEYDEALCVESTVTIGVQVNGKLRGQLEIEKDAPEDKAKEAAMAIPGVEQFAGGKEMKKFIYRPGKIINIVV 919          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A6U3T6T3_9STRA (Leucine--tRNA ligase n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6U3T6T3_9STRA)

HSP 1 Score: 1136 bits (2939), Expect = 0.000e+0
Identity = 560/880 (63.64%), Postives = 675/880 (76.70%), Query Frame = 1

Query:  253 TTRSVFEVENRSSVNSSAKRSPGNGHVSTASEAAAVATVGSRLYDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSET-VEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAEDI---ELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTLE-KPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 2865
            TT + +++  RS  +SS           T+S + A     S +Y   ++E +WQ  WE   TF+TP R   +PKK+VLDMFPYPSGAGLHVGHPEGYTASD+M+RYWRMKG+DVLHP+GWDSFGLPAEQ+AI+TG  P  TT  NID FRRQLKMLGFSYDW++E+ATTD  YVKWTQWIFL+LF  GLA+QS   VNWCPALGTVLANEE+I+G SERGDHPV RTPLRQWTLKIT YAD+LEAGL GLDWP GT+T+Q+QWIG+SEG +I F+++G  E  + VFTTR DTL GVTY+ LAPEHPL   I T E+++AV  YV  ++SRSD+DRT+    K+KTG  TG  AVHP+T +KVP+W  DYVLGSYGTGAVMAVPAHDERD  FA  +DL V+ VV+P   K  K   E +  EAF  +GV +NSGE FDGL+T +  +A+  +L+    G  +I Y+LRDW+FSRQRYWGEPIPIYFPVE    VDP+  DP++G EH+I Y+QPIPVD ++LPL LP M  F+PGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY RF D KN ++ +S K DE+WMPVDLYVGG EHAVLHLLYARFWHQVL DLG+ K PEPFQ LVHQGMILGTDGEKMSKSRGNVINPDDIV   GADALRLYEMFMGPLEA KPWQT QVAGV RF++++Y + +   ++    E+  ET  ILHKTMKKV+EDI+ L+FNTAISALMVLTNHL +L+   P+EA E L LMVSP APH+GEECW++LGHDK   +++  WV++ E LC+ ++V +GVQVNGKVR E+E++K A+ D          +VV   EGK+VKK IYVPGRI++ V K
Sbjct:   38 TTAASYKITTRSMSSSSVDEK-------TSSTSGATPEEDSGVYPFTQVEKKWQARWEAEKTFQTPVRDTSKPKKYVLDMFPYPSGAGLHVGHPEGYTASDIMSRYWRMKGYDVLHPIGWDSFGLPAEQYAINTGAQPVDTTKANIDNFRRQLKMLGFSYDWEKEIATTDVNYVKWTQWIFLQLFNKGLAKQSSVSVNWCPALGTVLANEEVINGLSERGDHPVERTPLRQWTLKITDYADRLEAGLDGLDWPSGTMTSQQQWIGKSEGCNIDFEIDGVDEEKITVFTTRADTLFGVTYVTLAPEHPLVSSITTAEQKEAVDAYVQKTSSRSDLDRTAA---KEKTGVFTGGYAVHPLTGEKVPIWVGDYVLGSYGTGAVMAVPAHDERDFEFAAKYDLDVKWVVEPQKNKKGK-KPELNTEEAFTVEGVNMNSGE-FDGLTTTKAKKAITYKLKEIGKGGPKITYKLRDWVFSRQRYWGEPIPIYFPVEFPEGVDPSTVDPKDGCEHEIKYDQPIPVDEADLPLELPLMTDFKPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPKNNDSAFSAKMDEEWMPVDLYVGGAEHAVLHLLYARFWHQVLFDLGYTKHPEPFQKLVHQGMILGTDGEKMSKSRGNVINPDDIVLGQGADALRLYEMFMGPLEAVKPWQTSQVAGVVRFQNKLYNVVKAAVKNENADEMNEETTKILHKTMKKVTEDIENLSFNTAISALMVLTNHLASLKGNVPKEAAEKLALMVSPIAPHIGEECWSMLGHDKS--LAYHEWVEYDEALCIDNTVTMGVQVNGKVRGEIEIAKDANQDXXXXXXXKQAKVVGQLEGKDVKKIIYVPGRILNIVAK 903          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S2RFM0_9STRA (Leucine--tRNA ligase n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2RFM0_9STRA)

HSP 1 Score: 1135 bits (2937), Expect = 0.000e+0
Identity = 559/851 (65.69%), Postives = 657/851 (77.20%), Query Frame = 1

Query:  349 AAAVATVGSRLYDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSE--TVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMV-----DPAGDPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAE--DIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQ--VVKFTEGKEVKKFIYVPGRIVSFVVK 2865
            AAA AT  S  Y  +++E +WQ  W E  TFKTPER   +PKK+VLDMFPYPSG GLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ AI+TG  P  TT  NID FRRQLKMLGFSYDWDRE+ATTD  YVKWTQWIFL+L+K GLA+QS   VNWCPALGTVLANEE+I+G SERGDHPV R PLRQW LKIT YAD+LEAGL+GLDWP GT+ AQKQWIG+SEG +I FD+EG+ E   V VFTTR DTL GVTY+ LAPEHPL  KI T ++++ V  YV  ++SRSDMDRT+    K+KTG  TGA A+HP+T +KVP+W  DYVLGSYGTGAVMAVPAHDERD  FA  F L V+ VV+P      K   E    EAF   GVA NS + FDGL+T +  +A+   L+    G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+ +     D      +G EH I Y+QPIPVD SELPL LP M  F+PGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY R+ D KN E   SKK DE+WMPVDLY+GG EHAVLHLLYARFWHQVL DLG+ + PEPF+ LVHQGMILGTDGEKMSKSRGNV+NPDDIV+A GADALRLYEMFMGPLEA KPWQT QV+GV RF++++Y + ++  E  D+++  ET  ILHKTMKKV+EDI+ ++FNTAISA+MVLTNHL +L E  PREA E L LMVSP APHLGEECW+LLG+DK   +++ PWV++ E LCV ++V +GVQVNGK R E+E+ K A  D+  A++EA+ Q  V    EGK++KK IYVPGRI++ V K
Sbjct:   72 AAAAATAASTNYPFEKVEKKWQDFWMENQTFKTPERDLSKPKKYVLDMFPYPSGTGLHVGHPEGYTASDVMARYWRMTGNDVLHPIGWDSFGLPAEQFAINTGAQPAETTTKNIDNFRRQLKMLGFSYDWDREIATTDVNYVKWTQWIFLQLYKKGLAQQSSVSVNWCPALGTVLANEEVINGLSERGDHPVERLPLRQWILKITDYADRLEAGLEGLDWPSGTMAAQKQWIGKSEGCNIDFDVEGSEEGDKVSVFTTRADTLFGVTYVTLAPEHPLISKITTADQKETVDDYVKKTSSRSDMDRTAS---KEKTGVFTGAYAIHPLTDEKVPIWVGDYVLGSYGTGAVMAVPAHDERDFEFASKFGLEVKWVVEP-----KKNPEEMSKEEAFTATGVATNSAD-FDGLTTAKTKKAITYNLKELNKGGPQITYKLRDWVFSRQRYWGEPIPIYFPVDFLEGVIPDDVDPKEDGCEHTIRYDQPIPVDESELPLELPHMEDFQPGDDPAGCLARAADWRYFQKDGKWFARETNTMPQWAGSCWYYFRYIDNKNDEEVISKKLDEEWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYTQHPEPFKKLVHQGMILGTDGEKMSKSRGNVVNPDDIVNAQGADALRLYEMFMGPLEAVKPWQTSQVSGVVRFQNKLYSVVKKAVENDDVKMDEETTKILHKTMKKVTEDIESMSFNTAISAMMVLTNHLQSLKENIPREAAEKLALMVSPIAPHLGEECWSLLGNDKS--LAYHPWVEYDESLCVDNTVTMGVQVNGKKRGEIEILKDA--DQETAMSEAMKQGRVESQLEGKDIKKIIYVPGRILNIVAK 909          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A448Z2U8_9STRA (Leucine--tRNA ligase n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z2U8_9STRA)

HSP 1 Score: 1133 bits (2931), Expect = 0.000e+0
Identity = 546/844 (64.69%), Postives = 659/844 (78.08%), Query Frame = 1

Query:  361 ATVGSRL-YDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGD-EHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAED--IELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 2865
            +TV ++  Y  +E+E +WQ++WEE  TFKTPER   +PKK+VLDMFPYPSGAGLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ A+ TGT P  TT  NI  FRRQLKMLGFSYDWD+ELATTDT YVKWTQWIFL+L+K GLAEQSE  VNWC ALGTVLANEE+I+G SERGDHPV R PLRQW LKIT+YAD+LE+GL GL+WP GT+T+Q+QWIG+S G  I FD+EG+ E+V+VFTTRPDTL+GVTY+ LAPEHPL   I T E++ +V KYV  ++SRSD+DRTS    K KTG  TGA   HPI+  +VPVW  DYVLGSYGTGAVMAVPAHD RD  FA+ FDL ++ VV+P  G+ NK        EAF   GVA+NSGE FDGL+T+ C +A+ ++LE    G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+    VDP   DP+N D EH I Y+QPIPVD ++LPL LP M +FEPGDDP GCL R  DWR+F++DG+WFARETNTMPQWAGSCWYY RF D  NTE  +S++ D+DWMPVDLY+GG EHAVLHLLYARFWHQVL DLG+ + PEPF+ LVHQGMILG+DGEKMSKSRGNV+NPDD+V   GADALRLYEMFMGPLEA KPWQTEQV GV RF++R+Y +    A+    ++  ET  ILHKTMKKV+EDI+ ++FNTAISA+MVLTNHL++L +K P EA E L LMVSP APHLGEECW++LGH   D +++  WV++ E LCV + V +G QVNGK+R ++ +   A  + A + A+ +  V    +GK ++K IYVPGRI++ +  
Sbjct:   59 STVAAKTGYPFQEVEPKWQKYWEENNTFKTPERDTSKPKKYVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMTGHDVLHPIGWDSFGLPAEQFAVQTGTQPAETTGKNIANFRRQLKMLGFSYDWDKELATTDTAYVKWTQWIFLQLYKKGLAEQSEVSVNWCAALGTVLANEEVINGLSERGDHPVQRLPLRQWVLKITEYADRLESGLDGLNWPSGTMTSQQQWIGKSVGCEIVFDVEGSDESVKVFTTRPDTLLGVTYVTLAPEHPLVSSITTPEQKDSVDKYVLETSSRSDLDRTSS---KVKTGVFTGAYVTHPISGDRVPVWVGDYVLGSYGTGAVMAVPAHDSRDFEFAKKFDLDMKWVVEPKKGELNKD-------EAFTEPGVAVNSGE-FDGLATKNCKKAITNKLEEINKGGAQITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVDPTSQDPKNDDCEHTIRYDQPIPVDEADLPLELPHMENFEPGDDPAGCLARAKDWRYFEKDGKWFARETNTMPQWAGSCWYYFRFLDPSNTEEAFSQQMDKDWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYSEHPEPFEKLVHQGMILGSDGEKMSKSRGNVVNPDDVVSEQGADALRLYEMFMGPLEAVKPWQTEQVPGVVRFQNRLYNVVNAAAKSKTTDMDEETARILHKTMKKVTEDIESMSFNTAISAMMVLTNHLVSLGDKVPLEAAENLALMVSPLAPHLGEECWSILGHK--DSLAYATWVEYDEALCVDNEVTIGCQVNGKMRGQITIPAEADEETAVSAAKEMENVQSQIDGKTIRKIIYVPGRILNIIAN 889          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S4I9K5_9STRA (Leucine--tRNA ligase n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4I9K5_9STRA)

HSP 1 Score: 1130 bits (2922), Expect = 0.000e+0
Identity = 556/849 (65.49%), Postives = 651/849 (76.68%), Query Frame = 1

Query:  346 EAAAVATVGSRLYDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTS-ETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGD-EHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAED--IELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 2865
            +A A A      Y   +IE +WQ  WEE  TFKTPER   + KK+VLDMFPYPSGAGLHVGHPEGYTASDVM+RYWRM GFDVLHP+GWDSFGLPAEQ AI+TG  P  TT  NI  F+RQLKMLGFSYDWDRE+ATTD EYVKWTQWIFL+L+K GLAEQS   VNWCPALGTVLANEE+I+G SERGDHPV R PLRQW LKIT YAD+LEAGL GLDWP GTLTAQ+QWIG+S G +I FD++G   E +  FTTR DTLMGVTY+ LAPEHPL   ++T E++  V  YV A++SRSD+DRT+    K+KTG  TG  A+HPI+  KVP+W  DYVLGSYGTGAVMAVPAHD+RD  FA  ++L V+ VVKP  G  N         EAF   GVA NSGE FDG++T  C + V  +LE+   G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+    V+P   DP+  D EH I ++QPIPV+ ++LPL LPEM  F PGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY RF D KN +A +S K DEDWMPVDLYVGG EHAVLHLLYARFWHQVL DLG+ K  EPFQ LVHQGMILG+DGEKMSKSRGNV+NPDDIVD  GADALRLYEMFMGPLEA KPWQTEQV+GV RF+++ Y + +  AE+   ++  ET  ILHKTMKKV+EDID ++FNTAISA+MVLTNHL TL +K P EA E L LMVSP APHLGEECW +LG++  D +++  WV++ EELCV ++V +GVQVNGKVR E+E+ K A  D A   A    +V    EGK++KK I+VPGRI++ V K
Sbjct:   60 DAPATAEAQPGGYPFAQIEEKWQAFWEENNTFKTPERNPEKAKKYVLDMFPYPSGAGLHVGHPEGYTASDVMSRYWRMNGFDVLHPIGWDSFGLPAEQFAINTGARPADTTAKNIANFKRQLKMLGFSYDWDREIATTDVEYVKWTQWIFLQLYKTGLAEQSSVSVNWCPALGTVLANEEVINGLSERGDHPVTRLPLRQWLLKITDYADRLEAGLDGLDWPSGTLTAQQQWIGKSVGCNIEFDVDGLEGEKITAFTTRADTLMGVTYVTLAPEHPLVSSVSTAEQKDEVDAYVKATSSRSDLDRTAA---KEKTGVFTGGYAIHPISGDKVPIWVGDYVLGSYGTGAVMAVPAHDQRDFEFATKYNLDVKWVVKPKKGDANTD-------EAFTANGVAANSGE-FDGMTTTTCKKEVTKKLESMDKGGAKITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVNPEDTDPKGDDCEHTIRFDQPIPVEEADLPLRLPEMDDFTPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPKNDDAAFSPKVDEDWMPVDLYVGGAEHAVLHLLYARFWHQVLFDLGYTKHSEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDDIVDRQGADALRLYEMFMGPLEAVKPWQTEQVSGVVRFQNKFYNVVKAAAENGCTDMDEETTKILHKTMKKVTEDIDSMSFNTAISAMMVLTNHLQTLKDKVPVEAAEKLALMVSPLAPHLGEECWNMLGNE--DSLAYHQWVEYDEELCVDNTVKMGVQVNGKVRGEIEIPKDADQDLAMEEANKQSKVASQLEGKDIKKIIFVPGRILNIVAK 895          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A1Z5K7R9_FISSO (Leucine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K7R9_FISSO)

HSP 1 Score: 1127 bits (2914), Expect = 0.000e+0
Identity = 544/837 (64.99%), Postives = 657/837 (78.49%), Query Frame = 1

Query:  379 LYDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGT-SETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGD-EHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQL--SRRGAEDIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTLEKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 2865
            ++  + +EA+WQ +W++  TF+TP+R   +PKK+VLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKG+DVLHP+GWDSFGLPAEQ+AI TG  P  TT  NI  F+RQL+MLGFSYDWDRELATTD EYVKWTQWIFL+LF+ GLAEQSE  VNWCPALGTVLANEE+I+G SERGDHPV R PLRQW LKIT+YAD+LE GL+GL+WP GTL+AQKQWIGRSEG  I F +EG  +ET+ VFTTR DTLMGVTY+ LAPEHPL L + ++E++ AV+ YV  ++SRSD+DRTS    K+K+G  TG  A+HPI+ ++VP+W  DYVLGSYGTGAVMAVPAHD+RD  FA+ + L ++ VV+PA         E D   AF   GV +NSGE FDGL+TE+  EAV  +LE  + G  +INY+LRDW+FSRQRYWGEPIPIYFP++    VDP+  DP+  D +H I Y+QPIPVD ++LPL LP M  F+PGD+P GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY RF D KN++  +SKK DE+WMPVDLYVGG EHAVLHLLYARFWHQVL DLG+   PEPFQ LVHQGMILG+DGEKMSKSRGNV+NPDDIV + GADALR+YEMFMGPLEA KPWQT QVAGV RF ++ Y +  S    +  E+  ETL +++KTMKKV+EDI+ ++FNTAISALMV  NHL +LE  PREA + L LMVSPFAPHLGEECW +LGH   + +++ PWV + E LCV  +V +GVQVNGK R E+ ++K A    A A A  + +V    EGKE+KK IYVPGRI++ + K
Sbjct:   50 VFPFQHVEAKWQAYWQKHQTFRTPQRDLSKPKKYVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGYDVLHPIGWDSFGLPAEQYAIQTGAQPADTTAANIANFKRQLQMLGFSYDWDRELATTDVEYVKWTQWIFLQLFRKGLAEQSEVSVNWCPALGTVLANEEVINGLSERGDHPVERLPLRQWVLKITQYADRLEEGLQGLEWPAGTLSAQKQWIGRSEGCRIDFGIEGMDTETITVFTTRADTLMGVTYVTLAPEHPLVLSVTSEEQKDAVSAYVNEASSRSDLDRTSS---KEKSGVFTGGYALHPISGERVPIWVGDYVLGSYGTGAVMAVPAHDQRDFEFAQKYGLDIKWVVEPAD------HAELDQTVAFTEYGVNVNSGE-FDGLTTEKAKEAVTKKLEELEKGGTQINYKLRDWVFSRQRYWGEPIPIYFPIDFPEGVDPSQQDPKLDDCQHTIRYDQPIPVDEADLPLKLPVMEDFQPGDNPAGCLARARDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPKNSKEAFSKKVDEEWMPVDLYVGGAEHAVLHLLYARFWHQVLFDLGYTTHPEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDDIVQSQGADALRMYEMFMGPLEAVKPWQTSQVAGVVRFMNKAYNIVVSAAATKHTEMGDETLRLMNKTMKKVTEDIENMSFNTAISALMVYANHLQSLETIPREAAKTLTLMVSPFAPHLGEECWNILGHT--ESLAYEPWVVFDEALCVDTTVTIGVQVNGKSRGEISIAKDADEATAVAAASGVDKVQVQLEGKEIKKIIYVPGRILNLIAK 874          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: W7TSV9_9STRA (Leucine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TSV9_9STRA)

HSP 1 Score: 1125 bits (2911), Expect = 0.000e+0
Identity = 552/838 (65.87%), Postives = 648/838 (77.33%), Query Frame = 1

Query:  382 YDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTSE---TVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVD-PAGDPRNGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQR-DGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQL--SRRGAEDIE-LAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMT--LEKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 2865
            Y+H+ IE +WQ++W ER TFK   R  G+ KK+VLDMFPYPSG+GLHVGHPEGYTASDVMARYWRM   DVLHPMGWDSFGLPAEQHAI+TGTHP VTTY NI  FRRQLK LGFSYDW RELATTD +YV+WTQWIFL+L+K GLAEQSE  VNWCPALGTVLANEEIIDGKSERGDHPV R PLRQW LKITKYA++LE  LKGLDWPEGT+TAQK WIGRSEGA + F L  +     +VEVFTTRPDTLMGVTY+VLAPEHPL  ++ ++ERR AV  YV  +A +SD+DRT+ GK K KTG DTGA  VHP++ +++P+W ADYVL  YGTGAVMAVPAHDERD AFA  F LP+++VV P AG       E    EAF G+G  +NSG   DG  T E  + VV +LE  + G   +NYRLRDW+FSRQRYWGEPIPIYFPV+M D   GDPR  D H I ++QPIPV+ +ELPL LP+M +F PG+DPQGCL R  DWRFF++ DG+W+ARETNTMPQWAGSCWYYLRF+D  N +  W ++ADE W+PVDLYVGGQEHAVLHLLYARFWH+ L DLG  K  EPF  LVHQGMILGTDGEKMSKSRGNV+NPDD+V   G DALRLYEMFMGPLEA KPWQT QVAGV RFRD+V+QL  +R   +  E +  ETL ++HKT+KKV++D++ L+FNTAIS +M+  NHL +   E  PR A+E LVLMVSPFAPHLGEECW +LGHD+   ++  PW +++EEL V D V LGVQVNGK R E+ +   A+  +  A A    +V  F +GK V K IYVPGRI++ V K
Sbjct:   75 YEHEVIEPKWQRYWAERETFKAIRRP-GKGKKYVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMCDLDVLHPMGWDSFGLPAEQHAINTGTHPGVTTYENIANFRRQLKSLGFSYDWSRELATTDWDYVRWTQWIFLQLYKKGLAEQSEVLVNWCPALGTVLANEEIIDGKSERGDHPVERLPLRQWVLKITKYAEELEEDLKGLDWPEGTMTAQKLWIGRSEGARVMFPLACSGAIPGSVEVFTTRPDTLMGVTYVVLAPEHPLVERVTSEERRAAVRAYVREAAGKSDLDRTATGKDKGKTGVDTGAKVVHPLSGEELPIWVADYVLWGYGTGAVMAVPAHDERDHAFATIFGLPIKQVVAPDAGSDALPVEE----EAFVGEGQVVNSGPLLDGQPTSEAKKVVVEELEKNERGAAVVNYRLRDWVFSRQRYWGEPIPIYFPVDMADHDTGDPRKDDPHTIRFDQPIPVEEAELPLKLPDMDNFAPGNDPQGCLARAKDWRFFKKSDGKWYARETNTMPQWAGSCWYYLRFSDPTNLKRAWGEEADEQWLPVDLYVGGQEHAVLHLLYARFWHKFLFDLGLTKHREPFLKLVHQGMILGTDGEKMSKSRGNVVNPDDVVKEVGGDALRLYEMFMGPLEAVKPWQTAQVAGVVRFRDKVFQLVRNREMTDSTEAMDDETLRLMHKTIKKVTQDVETLSFNTAISTMMIFANHLASRKTETLPRAAVENLVLMVSPFAPHLGEECWEMLGHDQS--LACHPWPRYQEELTVDDVVKLGVQVNGKTRGEVSVPADATEADVMAAAMEQEKVASFLDGKRVVKVIYVPGRILNIVAK 905          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S4AJT1_9STRA (Leucine--tRNA ligase n=2 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4AJT1_9STRA)

HSP 1 Score: 1125 bits (2911), Expect = 0.000e+0
Identity = 546/849 (64.31%), Postives = 657/849 (77.39%), Query Frame = 1

Query:  346 EAAAVATVGSRLYDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGTS-ETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPAG-DPRNGD-EHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGA--EDIELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVVK 2865
            E ++  T     Y   E+E +WQ++WEE  TFKTPER   +PKK+VLDMFPYPSGAGLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ+A+ TGT P  TT  NI  F RQLKMLGFSYDWD+ELATTDT YVKWTQWIFL+L+K GLAEQSE  VNWCPALGTVLANEE+I+G SERGDHPV R PLRQW LKIT+YAD+LEAGL GL+WP GT+T+Q+ WIG+S G  I FD+EG S ETV+VFTTR DTL+GVTY+ LAPEHPL   I T E+++ V  YVA ++SRSD+DRTS    K+KTG  TGA   HPI+  +VPVW  DYVLGSYGTGAVMAVPAHD RD  FA+ F+L V+ VV+P  G+ NK        EAF   GVA+NSGE FDGL+T+ C +A+ S+LE    G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+    VDPA  DP+N D EH I Y+Q IPVD ++LPL LP M +FEPGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYY RF D  N +  +SK+ D+DWMPVDLY+GG EHAVLHLLYARFWHQVL DLG+ + PEPF+ LVHQGMILGTDGEKMSKSRGNV+NPDD+V   GADALR+YEMFMGPLEA KPWQT QV GV RF++R+Y +    A  +  E+  ET  ILHKTMKKV+EDI+ ++FNTAISA+MVLTNHL++L +K P EA E L LMVSP APHLGEECW +LG++  + +++ PWV++ E LCV D + +GVQVNGK+R ++ ++  A  + A + A+ +  V    +GK ++K IY+ GRI++ +  
Sbjct:   55 EESSTNTPAKTGYPFAEVEPKWQKYWEENNTFKTPERDTSKPKKYVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMTGHDVLHPIGWDSFGLPAEQYAVQTGTQPAATTKKNIANFMRQLKMLGFSYDWDKELATTDTNYVKWTQWIFLQLYKKGLAEQSEVSVNWCPALGTVLANEEVINGLSERGDHPVKRLPLRQWVLKITEYADRLEAGLDGLNWPSGTMTSQQAWIGKSIGCEIDFDVEGDSGETVKVFTTRADTLLGVTYVTLAPEHPLVSSITTPEQKEVVDSYVATTSSRSDLDRTSS---KEKTGVFTGAFVTHPISGDRVPVWVGDYVLGSYGTGAVMAVPAHDTRDFEFAKKFELDVKWVVEPKKGELNK-------EEAFTAPGVAVNSGE-FDGLATKNCKKAITSKLEEINKGGAQITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVDPASVDPKNDDCEHTILYDQAIPVDEADLPLELPYMENFEPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPNNNDEAFSKQMDKDWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYSEHPEPFEKLVHQGMILGTDGEKMSKSRGNVVNPDDVVGENGADALRMYEMFMGPLEAVKPWQTGQVPGVVRFQNRLYNVVNAAARSQTNEMDEETTRILHKTMKKVTEDIELMSFNTAISAMMVLTNHLVSLGDKVPLEAAEKLALMVSPLAPHLGEECWNILGNE--ESLAYAPWVEYDESLCVDDEITMGVQVNGKMRGKITIAADADEEAAVSAAKEMNTVQSQIDGKTIRKIIYIQGRILNIIAN 890          

BLAST of mRNA_P-lacustris_contig1012.219.1 vs. uniprot
Match: A0A7S2XSK2_9STRA (Leucine--tRNA ligase n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2XSK2_9STRA)

HSP 1 Score: 1120 bits (2898), Expect = 0.000e+0
Identity = 554/849 (65.25%), Postives = 659/849 (77.62%), Query Frame = 1

Query:  343 SEAAAVATVGSRLYDHKEIEARWQQHWEERGTFKTPERTEGRPKKFVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAISTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWTLKITKYADKLEAGLKGLDWPEGTLTAQKQWIGRSEGASITFDLEGT-SETVEVFTTRPDTLMGVTYLVLAPEHPLTLKIATDERRQAVAKYVAASASRSDMDRTSMGKGKKKTGTDTGAVAVHPITKKKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARAFDLPVRRVVKPAAGKGNKGAVEGDVLEAFAGKGVAINSGEGFDGLSTEECAEAVVSQLEAEQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---VDPA-GDPR-NGDEHKICYEQPIPVDVSELPLTLPEMASFEPGDDPQGCLTRVPDWRFFQRDGQWFARETNTMPQWAGSCWYYLRFTDTKNTEAGWSKKADEDWMPVDLYVGGQEHAVLHLLYARFWHQVLHDLGHVKDPEPFQCLVHQGMILGTDGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLSRRGAED--IELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTL-EKPPREALEALVLMVSPFAPHLGEECWALLGHDKGDGVSFVPWVQWKEELCVSDSVMLGVQVNGKVRAELELSKTASSDEARALAEALPQVVKFTEGKEVKKFIYVPGRIVSFVV 2862
            S A + A VG   Y   EIE RWQ  W++  TFKTP R   +PKK+VLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKG+DVLHP+GWDSFGLPAEQ AI+TGT P VTT +NI  F+RQLKMLGFSYDWDRELATTD E+VKWTQWIFL+LFK GLAEQS   VNWCP LGTVLANEE+I+G SERGD PV+R PLRQW LKIT YAD+LE GL+GLDWP GT+ AQKQWIG+S G++I F ++G  +E + VFTTR DTLMGVTY+ LAPEHPL L I ++E+++ V  YV  ++SRSD+DRT+    K+KTG  TG   +HPI+ +KVP+W  DYVLGSYGTGAVMAVPAHD RD  FA+ F L ++ VV+P+  K  K        EAF   G  INSGE FDGL+T +  +A+  +L     G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+    V+P+  DP+ +G EH I Y+QP PVD +ELPLTLPE+  F+PGDDP GCL R  DWR+FQ+DG+WFARETNTMPQWAGSCWYYLR+ D  N+   +SK+AD DWMPVDLYVGG EHAVLHLLYARFWHQV+ DLG+ + PEPFQ LVHQGMILG+DGEKMSKSRGNV+NPD+IVDA GADALRLYEMFMGPLEA KPWQT QV+GV RF++++Y +     E+   E+  ET  ILHKTMKKVSEDID L FNTAISALMVLTNHL++L +K P EA E L LMVSPFAPHLGEECW LLGH   + +++ PWV++ E LC+   V +GVQVNGKVR E+ ++K A  D A + A    +V    +GK++KK IYVPGRI++F+V
Sbjct:   74 SAAVSAAGVG---YPFAEIEKRWQAKWQDEQTFKTPIRNPDKPKKYVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGYDVLHPIGWDSFGLPAEQFAINTGTKPAVTTASNIANFKRQLKMLGFSYDWDRELATTDVEFVKWTQWIFLQLFKKGLAEQSSVSVNWCPELGTVLANEEVINGLSERGDFPVIRLPLRQWLLKITDYADRLEEGLEGLDWPSGTMAAQKQWIGKSIGSNIDFKVDGLDTEKISVFTTRADTLMGVTYVTLAPEHPLVLSICSEEQKEEVEAYVKETSSRSDLDRTAA---KEKTGVFTGGYGIHPISGEKVPIWVGDYVLGSYGTGAVMAVPAHDVRDFEFAQKFGLEIKWVVQPSQTKELK-----STEEAFTPHGACINSGE-FDGLTTAKAKKAITRKLAEMDMGGAKITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVNPSDADPKEDGCEHTIRYDQPFPVDEAELPLTLPEIDDFKPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYLRYLDPTNSGEAFSKQADADWMPVDLYVGGAEHAVLHLLYARFWHQVMFDLGYTQHPEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDEIVDAQGADALRLYEMFMGPLEAVKPWQTSQVSGVVRFQNKLYTVVNTAIENQVTEMDDETTRILHKTMKKVSEDIDTLGFNTAISALMVLTNHLVSLKDKVPLEAAEKLALMVSPFAPHLGEECWKLLGHT--EMLAYHPWVEYDESLCIDTDVTIGVQVNGKVRGEIFIAKDADQDLAMSEARKQERVAFQLDGKDIKKVIYVPGRILNFIV 908