mRNA_P-lacustris_contig99.16178.1 (mRNA) Pleurocladia lacustris SAG_25_93

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-lacustris_contig99.16178.1
Unique NamemRNA_P-lacustris_contig99.16178.1
TypemRNA
OrganismPleurocladia lacustris SAG_25_93 (Pleurocladia lacustris SAG_25_93)
Homology
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: D8LNR1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNR1_ECTSI)

HSP 1 Score: 222 bits (565), Expect = 1.640e-70
Identity = 116/149 (77.85%), Postives = 132/149 (88.59%), Query Frame = 1 
Query:    1 MDVSSHTVPAAFCFIAVKLTWQSVQSCTPKRSKRGKLKGQGTTQ-QQQATQGSA-DSQGVHLSTDVSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLACERFL 441
            M+ SS+T+PAAFCFIAV+LT+Q+ +  TPKR KRGK+KG GTTQ QQQA QGSA  SQGV+L TDV+FK+ IL A+K LHGEVGS AYPVDVLSWDAV+GEGRLRVPARS+VPIRAALS VGSCG+VPCAIDV EVSPFLAGLACER+L
Sbjct:    1 MEASSYTLPAAFCFIAVQLTFQATRQATPKRPKRGKVKGGGTTQRQQQAPQGSAATSQGVYLPTDVAFKTFILAAVKDLHGEVGSLAYPVDVLSWDAVSGEGRLRVPARSLVPIRAALSFVGSCGDVPCAIDVREVSPFLAGLACERYL 149
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: A0A8J6EM83_ELECQ (Uncharacterized protein n=1 Tax=Eleutherodactylus coqui TaxID=57060 RepID=A0A8J6EM83_ELECQ)

HSP 1 Score: 67.8 bits (164), Expect = 9.140e-11
Identity = 36/79 (45.57%), Postives = 51/79 (64.56%), Query Frame = 1 
Query:  190 VSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLA 426
            V  KSLIL+ALK LHGEVG+ +YP+DVL +D       LR+ +  +V +  +L++VG      C+I V++ SPFL  LA
Sbjct:   43 VQLKSLILSALKELHGEVGA-SYPLDVLKFDGKTSSAILRIKSSGLVKLWTSLTMVGQYQGQLCSIRVLQTSPFLLALA 120
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: Q07G50_XENTR (Ribonuclease P 14kDa subunit n=3 Tax=Xenopus TaxID=8353 RepID=Q07G50_XENTR)

HSP 1 Score: 65.1 bits (157), Expect = 1.060e-9
Identity = 39/87 (44.83%), Postives = 57/87 (65.52%), Query Frame = 1 
Query:  166 QGVHLSTDVSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLA 426
            +G  LS    FK L+++ALK LHGEVGS + P+D+LS+D       LRV +R +V + ++L+L+G   N  C+I V++ SPFL  LA
Sbjct:   43 EGAQLSA-TQFKLLVVSALKNLHGEVGS-SLPLDLLSFDEKTLCAVLRVCSRGLVKLWSSLTLLGHYKNHECSIRVIQTSPFLLALA 127
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: A0A7D9IBD1_PARCT (Ribonuclease P subunit p14 n=3 Tax=Paramuricea clavata TaxID=317549 RepID=A0A7D9IBD1_PARCT)

HSP 1 Score: 64.7 bits (156), Expect = 1.440e-9
Identity = 34/81 (41.98%), Postives = 52/81 (64.20%), Query Frame = 1 
Query:  190 VSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLACE 432
            V  KSLI+ ALK LHG VG+ A  VDVL  +  +GE  LRV +  ++ +R++L+++ +    PC  +V +VSP+L  LA +
Sbjct:   36 VKLKSLIVNALKQLHGLVGA-AITVDVLKCEKTSGEIILRVKSSDLIKLRSSLTILNNYNGTPCRCNVKQVSPYLMSLAAD 115
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: A0A0E9XE39_ANGAN (Uncharacterized protein n=1 Tax=Anguilla anguilla TaxID=7936 RepID=A0A0E9XE39_ANGAN)

HSP 1 Score: 63.5 bits (153), Expect = 3.360e-9
Identity = 33/77 (42.86%), Postives = 53/77 (68.83%), Query Frame = 1 
Query:  196 FKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLA 426
            FK ++++AL+ L+GE+G+ A+P DVL ++       LRV    +V + +AL+L+GS  + PCA+ VV+VSPFL  L+
Sbjct:   45 FKQIVISALRDLYGEIGA-AFPFDVLKFEEDTLTAVLRVYNSGLVKLWSALTLLGSYHSEPCAVRVVQVSPFLLALS 120
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: UPI001AACA93D (ribonuclease P protein subunit p14-like isoform X2 n=1 Tax=Rana temporaria TaxID=8407 RepID=UPI001AACA93D)

HSP 1 Score: 63.2 bits (152), Expect = 4.660e-9
Identity = 37/87 (42.53%), Postives = 56/87 (64.37%), Query Frame = 1 
Query:  166 QGVHLSTDVSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLA 426
            +GV L T   +KSLI++A+K LHGEVG+ + P+D+L +D       LR+ +   V +  +L+L+G   N  C+I V++VSPFL  LA
Sbjct:   36 EGVKL-TAAQYKSLIVSAVKELHGEVGA-SCPLDLLKYDDKTLSAILRINSSGFVKLWTSLTLLGHYQNQECSIRVLQVSPFLLALA 120
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: UPI0008550BAE (ribonuclease P protein subunit p14 n=1 Tax=Nanorana parkeri TaxID=125878 RepID=UPI0008550BAE)

HSP 1 Score: 62.4 bits (150), Expect = 8.940e-9
Identity = 35/87 (40.23%), Postives = 56/87 (64.37%), Query Frame = 1 
Query:  166 QGVHLSTDVSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLA 426
            +GV L T   +KSLI++A+K LHGEVG+ + P+D+L ++       LR+ +   V +  +L+L+G   N  C++ V++VSPFL  LA
Sbjct:   36 EGVKL-TAAQYKSLIVSAVKELHGEVGA-SCPLDLLKYEEKTLSAILRIRSSGFVKLWTSLTLLGQYQNQACSVCVIQVSPFLLALA 120
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: A0A2B4T178_STYPI (Ribonuclease P protein subunit p14 n=1 Tax=Stylophora pistillata TaxID=50429 RepID=A0A2B4T178_STYPI)

HSP 1 Score: 60.1 bits (144), Expect = 6.010e-8
Identity = 33/81 (40.74%), Postives = 52/81 (64.20%), Query Frame = 1 
Query:  190 VSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLACE 432
            V+FKS+++  L ALHG+VGS A  VD++ ++A   E  LRV    +V + +AL+L+    +  CAI V++VSP+L  L  +
Sbjct:   35 VTFKSILIQTLDALHGKVGS-AISVDIIKFEASLKEAILRVKESGLVKLWSALTLLSYYKDKRCAIYVIQVSPYLLSLGAD 114
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: A0A1A8LBW7_9TELE (Uncharacterized protein n=8 Tax=Nothobranchius TaxID=28779 RepID=A0A1A8LBW7_9TELE)

HSP 1 Score: 59.7 bits (143), Expect = 8.680e-8
Identity = 36/78 (46.15%), Postives = 49/78 (62.82%), Query Frame = 1 
Query:  190 VSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGL 423
            V  KSLI+T LK LHGEVG+ A P DVL +D  +    LRV +  +V + ++L+L G   +  CA  V++VSPFL  L
Sbjct:   43 VELKSLIITGLKDLHGEVGA-ALPFDVLKFDEGSLTAVLRVCSGGLVKLWSSLTLQGYYQSQACAFRVLQVSPFLLAL 119
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BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Match: UPI001F047F46 (ribonuclease P protein subunit p14-like n=1 Tax=Xenia sp. Carnegie-2017 TaxID=2897299 RepID=UPI001F047F46)

HSP 1 Score: 58.2 bits (139), Expect = 3.030e-7
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = 1 
Query:  190 VSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEGRLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLA 426
            V  KS I++A+K LHG VGS +  +D+L  D  NGE  LRV + ++V + ++L+L+    +  C  +V +VSP+L  LA
Sbjct:   37 VQLKSFIVSAVKQLHGSVGS-STVIDILKVDKRNGEIILRVKSSNLVKLWSSLTLINKYNDNACRFNVQQVSPYLMSLA 114
The following BLAST results are available for this feature:
BLAST of mRNA_P-lacustris_contig99.16178.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 12
Match NameE-valueIdentityDescription
D8LNR1_ECTSI1.640e-7077.85Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A8J6EM83_ELECQ9.140e-1145.57Uncharacterized protein n=1 Tax=Eleutherodactylus ... [more]
Q07G50_XENTR1.060e-944.83Ribonuclease P 14kDa subunit n=3 Tax=Xenopus TaxID... [more]
A0A7D9IBD1_PARCT1.440e-941.98Ribonuclease P subunit p14 n=3 Tax=Paramuricea cla... [more]
A0A0E9XE39_ANGAN3.360e-942.86Uncharacterized protein n=1 Tax=Anguilla anguilla ... [more]
UPI001AACA93D4.660e-942.53ribonuclease P protein subunit p14-like isoform X2... [more]
UPI0008550BAE8.940e-940.23ribonuclease P protein subunit p14 n=1 Tax=Nanoran... [more]
A0A2B4T178_STYPI6.010e-840.74Ribonuclease P protein subunit p14 n=1 Tax=Styloph... [more]
A0A1A8LBW7_9TELE8.680e-846.15Uncharacterized protein n=8 Tax=Nothobranchius Tax... [more]
UPI001F047F463.030e-740.51ribonuclease P protein subunit p14-like n=1 Tax=Xe... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-lacustris_contig99contigP-lacustris_contig99:51606..53440 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Pleurocladia lacustris SAG_25_932021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score72.0
Seed ortholog evalue2.6e-10
Seed eggNOG ortholog28377.ENSACAP00000011629
Preferred nameRPP14
KEGG koko:K14529
KEGG Pathwayko03013,map03013
Hectar predicted targeting categoryother localisation
GOsGO:0000966,GO:0001682,GO:0003674,GO:0003676,GO:0003723,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0006139,GO:0006396,GO:0006399,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009987,GO:0010467,GO:0016070,GO:0030677,GO:0030681,GO:0031974,GO:0031981,GO:0032991,GO:0034470,GO:0034471,GO:0034641,GO:0034660,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0046483,GO:0070013,GO:0071704,GO:0090304,GO:0097159,GO:0099116,GO:1901360,GO:1901363,GO:1902494,GO:1902555,GO:1905348,GO:1990904
EggNOG free text desc.tRNA 5'-leader removal
EggNOG OGs2BCSB@1,2S10S@2759,3A3XP@33154,3BRFU@33208,3D7R7@33213,48EKZ@7711,49BQG@7742
Ec32 ortholog descriptionRibonuclease P/MRP protein subunit
Ec32 orthologEc-08_006170.1
EC3.1.26.5
COG Functional cat.S
Best tax levelVertebrata
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03016
Exons3
Model size753
Cds size444
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-lacustris_contig99.16178.1prot_P-lacustris_contig99.16178.1Pleurocladia lacustris SAG_25_93polypeptideP-lacustris_contig99 51915..53440 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622817420.1873727-UTR-P-lacustris_contig99:51605..519141622817420.1873727-UTR-P-lacustris_contig99:51605..51914Pleurocladia lacustris SAG_25_93UTRP-lacustris_contig99 51606..51914 -
1693495054.718316-UTR-P-lacustris_contig99:51605..519141693495054.718316-UTR-P-lacustris_contig99:51605..51914Pleurocladia lacustris SAG_25_93UTRP-lacustris_contig99 51606..51914 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622817420.199632-CDS-P-lacustris_contig99:51914..520381622817420.199632-CDS-P-lacustris_contig99:51914..52038Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig99 51915..52038 -
1693495054.732515-CDS-P-lacustris_contig99:51914..520381693495054.732515-CDS-P-lacustris_contig99:51914..52038Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig99 51915..52038 -
1622817420.2109385-CDS-P-lacustris_contig99:52706..529701622817420.2109385-CDS-P-lacustris_contig99:52706..52970Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig99 52707..52970 -
1693495054.744919-CDS-P-lacustris_contig99:52706..529701693495054.744919-CDS-P-lacustris_contig99:52706..52970Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig99 52707..52970 -
1622817420.2215266-CDS-P-lacustris_contig99:53384..534401622817420.2215266-CDS-P-lacustris_contig99:53384..53440Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig99 53385..53440 -
1693495054.7549586-CDS-P-lacustris_contig99:53384..534401693495054.7549586-CDS-P-lacustris_contig99:53384..53440Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig99 53385..53440 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-lacustris_contig99.16178.1

>prot_P-lacustris_contig99.16178.1 ID=prot_P-lacustris_contig99.16178.1|Name=mRNA_P-lacustris_contig99.16178.1|organism=Pleurocladia lacustris SAG_25_93|type=polypeptide|length=148bp
MDVSSHTVPAAFCFIAVKLTWQSVQSCTPKRSKRGKLKGQGTTQQQQATQ
GSADSQGVHLSTDVSFKSLILTALKALHGEVGSWAYPVDVLSWDAVNGEG
RLRVPARSIVPIRAALSLVGSCGNVPCAIDVVEVSPFLAGLACERFL*
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mRNA from alignment at P-lacustris_contig99:51606..53440-

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-lacustris_contig99.16178.1 ID=mRNA_P-lacustris_contig99.16178.1|Name=mRNA_P-lacustris_contig99.16178.1|organism=Pleurocladia lacustris SAG_25_93|type=mRNA|length=1835bp|location=Sequence derived from alignment at P-lacustris_contig99:51606..53440- (Pleurocladia lacustris SAG_25_93)
ATGGACGTATCATCGCACACGGTTCCAGCCGCATTCTGCTTCATCGCAGT
CAAACTGTGAGATAGGGTGCAACGTTTTCAAGGCTTGAACATGTTTGCTT
GATACAGCAGTCTAGTGGTTCATCCTCGTTGTCTATGGTGTGTGGCCCAT
GCTTGTGCTTCGACAGCCAACTCACGGGTAAAGTCTGCTTGCAGTTTGTC
GCGTCATACACGATCCATCATGCCTCCAATTGCTGAAGGCACATGTCTGT
GCGTTGTATACAGACTATACGTATCCTCGGCCGCTTCAATCGATGCGGAT
ACGCCGACGCGGGTAGCTACGTTGGTGCTGCTTCAGCGAGACACTGCTTT
GCCTCTTGCGCAAGTGCTCACGCAAGCTAGCTTTCTGCCTCTGATAACTC
GTGCCTGGTCCTTCTCTGCGTCTCCTCCCTCGTCGCACGTTCAAACCAAC
CACAACACATCCACGGACAGAACTTGGCAAAGCGTACAAAGTTGTACTCC
TAAGCGATCCAAGAGAGGCAAACTCAAAGGTCAAGGGACGACCCAGCAGC
AGCAGGCAACGCAGGGGAGCGCCGACAGCCAAGGAGTCCACCTTTCCACA
GACGTTTCGTTCAAATCCTTGATACTCACAGCACTAAAAGCACTGCACGG
CGAGGTGGGGTCGTGGGCCTACCCTGTTGACGTTTTGTCGTGGGACGCGG
TCAACGGCGAGGGCCGCCTGAGGGTGCCTGCAAGGTAAAGAAAGACCGAA
AGTGTATCGGTGCTGTTGTGTCTTCCTAACGTATGGAAACAGAAACACAC
GGCGAAAGGCTGCACCTAAGTTGCGGTGCAAGCAAATACAGTGTTGGGCA
CGCCACCCGTTTCCCCTTTGCGTGCCGCCTCTGCTTTAAGCCAAGGTTTG
ACGGCAAAACCACGTCTTCGTTGTACCTTGTAGACTAGTGGCCTACGTGA
ACAGGCCGATGACATGACTAGAAATGTTTCGCCCTCCTAGGCCTTCGCTC
AGCACGGCGTAGGCAAGCTTGTCGCTATTGATGATGGCTTGAGGCGTGCC
GTGCTTGGGTGTGTGCAGTAGCTGCACAGGTGGAGTGGGGTGGCTTCATC
AGTGGAAGTTTTGCGATACACCCCTCACTCTTGCCCCCTCTGCCCTTCTA
CAGCTGACAATGTGTCTGACAGACGGCGTTGTATCGATGGAGTTGGTCAC
CAAGCAGATATTGCACGCTTTCTAAAATTCCACGTCAGGGGTAAGAATCA
TCCGGCTTTGCCTCCCCCCTCCGACCACGCGATCGATGATCGTTCAAGCA
ATGCATACATACAACTGACGGACAAATGCATTCATTTCCGTGCATGTGCT
GCTGTTCTCTTGCTGTCTCCTCTCTACCAACTGCGGTCGTAACCGTAGCC
AGGTCAATAGTTCCGATCCGTGCAGCGCTGAGTTTGGTGGGATCCTGCGG
GAATGTTCCGTGCGCGATAGACGTCGTGGAGGTGTCGCCATTTTTGGCAG
GCCTGGCATGCGAGCGATTTCTCTAATGTAAAGCAACCACCTCTCCGTCA
CTGGTTGACGCCAATCTTCGAATTATTCCGATTGATTTACATGTACGTCG
CTGGTTTAATGGACACATCTATCTGTGTCTCTAGTGACGCCGCCTCTGAT
CGAATGGGTGACTATTTTTTGTGCCTGCCTTCCGCCGGCAAAGCAAGGCT
AAGCCCTCCTACCCTCTTTCACGTATATCGGGTCGAGGTATCGTCTGTAC
GAAGTACCGTTTGTTCGGGGTACCATCTCGACAATTTGCGATGTTCTGGC
CTCCGAGGCTGAAGGTCTAGCTGCCGAGATGAATA
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Coding sequence (CDS) from alignment at P-lacustris_contig99:51606..53440-

>mRNA_P-lacustris_contig99.16178.1 ID=mRNA_P-lacustris_contig99.16178.1|Name=mRNA_P-lacustris_contig99.16178.1|organism=Pleurocladia lacustris SAG_25_93|type=CDS|length=888bp|location=Sequence derived from alignment at P-lacustris_contig99:51606..53440- (Pleurocladia lacustris SAG_25_93)
ATGGACGTATCATCGCACACGGTTCCAGCCGCATTCTGCTTCATCGCAGT
CAAACTATGGACGTATCATCGCACACGGTTCCAGCCGCATTCTGCTTCAT
CGCAGTCAAACTAACTTGGCAAAGCGTACAAAGTTGTACTCCTAAGCGAT
CCAAGAGAGGCAAACTCAAAGGTCAAGGGACGACCCAGCAGCAGCAGGCA
ACGCAGGGGAGCGCCGACAGCCAAGGAGTCCACCTTTCCACAGACGTTTC
GTTCAAATCCTTGATACTCACAGCACTAAAAGCACTGCACGGCGAGGTGG
GGTCGTGGGCCTACCCTGTTGACGTTTTGTCGTGGGACGCGGTCAACGGC
GAGGGCCGCCTGAGGGTGCCTGCAAGAACTTGGCAAAGCGTACAAAGTTG
TACTCCTAAGCGATCCAAGAGAGGCAAACTCAAAGGTCAAGGGACGACCC
AGCAGCAGCAGGCAACGCAGGGGAGCGCCGACAGCCAAGGAGTCCACCTT
TCCACAGACGTTTCGTTCAAATCCTTGATACTCACAGCACTAAAAGCACT
GCACGGCGAGGTGGGGTCGTGGGCCTACCCTGTTGACGTTTTGTCGTGGG
ACGCGGTCAACGGCGAGGGCCGCCTGAGGGTGCCTGCAAGGTCAATAGTT
CCGATCCGTGCAGCGCTGAGTTTGGTGGGATCCTGCGGGAATGTTCCGTG
CGCGATAGACGTCGTGGAGGTGTCGCCATTTTTGGCAGGCCTGGCATGCG
AGCGATTTCTCTAAGTCAATAGTTCCGATCCGTGCAGCGCTGAGTTTGGT
GGGATCCTGCGGGAATGTTCCGTGCGCGATAGACGTCGTGGAGGTGTCGC
CATTTTTGGCAGGCCTGGCATGCGAGCGATTTCTCTAA
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