mRNA_P-lacustris_contig101.199.1 (mRNA) Pleurocladia lacustris SAG_25_93

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-lacustris_contig101.199.1
Unique NamemRNA_P-lacustris_contig101.199.1
TypemRNA
OrganismPleurocladia lacustris SAG_25_93 (Pleurocladia lacustris SAG_25_93)
Homology
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: D7G1T0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1T0_ECTSI)

HSP 1 Score: 209 bits (532), Expect = 5.240e-64
Identity = 106/152 (69.74%), Postives = 120/152 (78.95%), Query Frame = 2 
Query:  212 MICTYLVGINALSFMMHAEDKASSVGWLPFVGRIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIVQVAASIVWGYFKGATWALAAAGLAPAVMFALTARHMRTFKYRQHRRDNGSYGDGRHQPWQRPVDVSAPAGRRRNT 667
            ++  Y VG NALSF M AEDKASS GW+PF+GRIPEALLHQVEL GGSPGSFLGQR+FRHK+SKL+YQR FSQVL VQ+AASI WGYF G +WAL   GLAPAVMFA TAR M T K+R + R+  +      QPWQRPVDVS+ A RRRNT
Sbjct:    2 ILAAYFVGANALSFAMLAEDKASSKGWIPFLGRIPEALLHQVELVGGSPGSFLGQRVFRHKISKLAYQRSFSQVLAVQIAASIAWGYFHGPSWALLGMGLAPAVMFAATARRMSTLKHRDNARNRWN------QPWQRPVDVSSSARRRRNT 147
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: A0A6H5K9I2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K9I2_9PHAE)

HSP 1 Score: 199 bits (505), Expect = 5.900e-60
Identity = 102/152 (67.11%), Postives = 119/152 (78.29%), Query Frame = 2 
Query:  212 MICTYLVGINALSFMMHAEDKASSVGWLPFVGRIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIVQVAASIVWGYFKGATWALAAAGLAPAVMFALTARHMRTFKYRQHRRDNGSYGDGRHQPWQRPVDVSAPAGRRRNT 667
            ++  + VG+NALSF M AEDKASS G +PF+GRIPEALLHQVELAGGSPGSFLGQR+F+HK+SKL+YQRGFSQVL  Q+AA+I WGYF G +WAL   GLAP VMFA TAR M T K++   R+        HQPWQRPVDVS+ A RRRNT
Sbjct:    2 ILAAFFVGVNALSFAMLAEDKASSKGLIPFLGRIPEALLHQVELAGGSPGSFLGQRVFQHKISKLAYQRGFSQVLAAQIAANIAWGYFHGPSWALLGVGLAPVVMFAATARRMNTLKHQDSTRNRW------HQPWQRPVDVSS-ARRRRNT 146
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: A0A1V5MWG7_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium ADurb.Bin412 TaxID=1852905 RepID=A0A1V5MWG7_9BACT)

HSP 1 Score: 70.5 bits (171), Expect = 2.120e-11
Identity = 33/85 (38.82%), Postives = 55/85 (64.71%), Query Frame = 2 
Query:  212 MICTYLVGINALSFMMHAEDKASSVGWLPFVGRIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIVQVAASIVW 466
            ++ +YL  I+ ++F+M+  DKA +V      GR+PE +LH + LAGG+PG+F GQ +FRHK  K  ++  F  + ++Q+ A I +
Sbjct:   32 LLVSYLASISGITFLMYGFDKARAV---KNQGRVPELILHGLALAGGTPGAFFGQLVFRHKTRKWKFRIVFILIFLLQIGALIYY 113
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: A0A7M2WUF6_9BACT (DUF1294 domain-containing protein n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7M2WUF6_9BACT)

HSP 1 Score: 64.3 bits (155), Expect = 4.100e-9
Identity = 35/89 (39.33%), Postives = 49/89 (55.06%), Query Frame = 2 
Query:  215 ICTYLVGINALSFMMHAEDKA-SSVGWLPFVGRIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIVQVAASIVWGYFK 478
            +  YL  IN  + +++  DK  +S G L    R+PE +LH     GG+PG+F GQRLFRHK  K S+QR F  +  +Q+     W Y  
Sbjct:   41 VWAYLAAINVTTLLLYGFDKRQASTGGL----RVPEKVLHTHAFVGGTPGAFCGQRLFRHKTIKGSFQRLFWGIFGLQLLLIAAWIYLS 125
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: A0A7V2IL43_9BACT (DUF1294 domain-containing protein n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7V2IL43_9BACT)

HSP 1 Score: 60.5 bits (145), Expect = 4.080e-8
Identity = 34/88 (38.64%), Postives = 48/88 (54.55%), Query Frame = 2 
Query:  224 YLVGINALSFMMHAEDKASSVGWLPFVGRIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIVQVAASIVWGYFKGAT 487
            YL  +  ++F     DK  ++      GRIPEA+LH + LAGGS G+  GQ LFRHK+ KL +    + ++ VQ+     W Y   AT
Sbjct:    7 YLTALTLVTFAFFGFDKHRAIRGR---GRIPEAVLHLLALAGGSLGALAGQILFRHKIRKLYFTAILAMIVAVQLGCIAWWIYRTRAT 91
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: UPI001904956F (DUF1294 domain-containing protein n=2 Tax=Haloferula rosea TaxID=490093 RepID=UPI001904956F)

HSP 1 Score: 62.0 bits (149), Expect = 1.150e-7
Identity = 31/45 (68.89%), Postives = 34/45 (75.56%), Query Frame = 2 
Query:  308 RIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIV 442
            RIPEA LH  EL GG PGSFLGQRL RHKVSK SYQ  F  +++V
Sbjct:  134 RIPEATLHLFELMGGWPGSFLGQRLLRHKVSKKSYQMIFWAIVLV 178
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: A0A259DYK7_9SPHN (Uncharacterized protein n=2 Tax=Sphingomonadales TaxID=204457 RepID=A0A259DYK7_9SPHN)

HSP 1 Score: 59.3 bits (142), Expect = 1.450e-7
Identity = 33/92 (35.87%), Postives = 50/92 (54.35%), Query Frame = 2 
Query:  197 LLQREMICTYLVGINALSFMMHAEDKASSVGWLPFVGR--IPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIVQVAASIVW 466
            LL  E + T L+ +NA +F+    DK     W    GR  + E  L Q+ L GG+PG++LG+R+FRHK  K  + R    +  +Q+ A + W
Sbjct:    8 LLTIENVVTALLALNATTFVSFGIDK-----WKARTGRWRVSEGTLLQLALLGGTPGAYLGRRVFRHKTRKQPFVRYLHTIAFLQIIAIVCW 94
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: A0A6A7YTS5_9PSED (DUF1294 domain-containing protein n=1 Tax=Pseudomonas helleri TaxID=1608996 RepID=A0A6A7YTS5_9PSED)

HSP 1 Score: 59.7 bits (143), Expect = 1.480e-7
Identity = 36/82 (43.90%), Postives = 47/82 (57.32%), Query Frame = 2 
Query:  239 NALSFMMHAEDK--ASSVGWLPFVGRIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIVQVAASIVWGYFK 478
            + L+F M+  DK  A +  W     RIPE +LH +EL GG PG+ L Q++FRHK  KLSYQ  F  ++ V  A  I   Y K
Sbjct:   39 SGLAFGMYGYDKRQAKAGEW-----RIPEKVLHAIELLGGWPGALLAQQVFRHKTRKLSYQLWFWLIVAVHQALWIDVLYLK 115
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BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: A0A191YT64_9PSED (Uncharacterized protein n=3 Tax=Pseudomonas TaxID=286 RepID=A0A191YT64_9PSED)

HSP 1 Score: 59.7 bits (143), Expect = 2.460e-7
Identity = 32/71 (45.07%), Postives = 46/71 (64.79%), Query Frame = 2 
Query:  236 INALSFMMHAEDK--ASSVGWLPFVGRIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIV 442
            ++ L+F+++  DK  A    W     RIPE LLH VELAGG PG+ L Q++FRHK  KLS+Q  F  ++++
Sbjct:   58 VSGLAFLLYWSDKRKARLDRW-----RIPENLLHAVELAGGWPGALLAQQVFRHKTRKLSFQLLFWMIVLL 123
[Close]
BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Match: A0A356NHF9_9BACT (DUF1294 domain-containing protein n=4 Tax=Verrucomicrobiales TaxID=48461 RepID=A0A356NHF9_9BACT)

HSP 1 Score: 58.5 bits (140), Expect = 3.060e-7
Identity = 31/74 (41.89%), Postives = 44/74 (59.46%), Query Frame = 2 
Query:  224 YLVGINALSFMMHAEDKASSVGWLPFVGRIPEALLHQVELAGGSPGSFLGQRLFRHKVSKLSYQRGFSQVLIVQ 445
            YL  IN + F  + EDK  ++       R PE +LH + LAGG  G+ LGQ++FRHK  K S+QR F  ++ +Q
Sbjct:   34 YLASINIICFGFYGEDKWRAIKQ---ESRAPEGMLHLMALAGGVLGARLGQKVFRHKTLKGSFQRAFWFIVFLQ 104
The following BLAST results are available for this feature:
BLAST of mRNA_P-lacustris_contig101.199.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G1T0_ECTSI5.240e-6469.74Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5K9I2_9PHAE5.900e-6067.11Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A1V5MWG7_9BACT2.120e-1138.82Uncharacterized protein n=1 Tax=Planctomycetes bac... [more]
A0A7M2WUF6_9BACT4.100e-939.33DUF1294 domain-containing protein n=1 Tax=Phycisph... [more]
A0A7V2IL43_9BACT4.080e-838.64DUF1294 domain-containing protein n=1 Tax=Phycisph... [more]
UPI001904956F1.150e-768.89DUF1294 domain-containing protein n=2 Tax=Haloferu... [more]
A0A259DYK7_9SPHN1.450e-735.87Uncharacterized protein n=2 Tax=Sphingomonadales T... [more]
A0A6A7YTS5_9PSED1.480e-743.90DUF1294 domain-containing protein n=1 Tax=Pseudomo... [more]
A0A191YT64_9PSED2.460e-745.07Uncharacterized protein n=3 Tax=Pseudomonas TaxID=... [more]
A0A356NHF9_9BACT3.060e-741.89DUF1294 domain-containing protein n=4 Tax=Verrucom... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-lacustris_contig101contigP-lacustris_contig101:207189..209082 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Pleurocladia lacustris SAG_25_932021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score64.3
Seed ortholog evalue5.9e-08
Seed eggNOG ortholog629265.PMA4326_07394
Preferred nameysdA
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Protein of unknown function (DUF1294)
EggNOG OGs1N6YM@1224,1SCMX@1236,1ZA06@136849,COG3326@1,COG3326@2
Ec32 ortholog descriptionProtein of unknown function DUF1294
Ec32 orthologEc-07_004270.1
COG Functional cat.S
Best tax levelPseudomonas syringae group
Best eggNOG OGNA|NA|NA
Exons3
Model size1045
Cds size483
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-lacustris_contig101.199.1prot_P-lacustris_contig101.199.1Pleurocladia lacustris SAG_25_93polypeptideP-lacustris_contig101 207400..208731 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815279.4937723-UTR-P-lacustris_contig101:207188..2073991622815279.4937723-UTR-P-lacustris_contig101:207188..207399Pleurocladia lacustris SAG_25_93UTRP-lacustris_contig101 207189..207399 +
1693493447.1506753-UTR-P-lacustris_contig101:207188..2073991693493447.1506753-UTR-P-lacustris_contig101:207188..207399Pleurocladia lacustris SAG_25_93UTRP-lacustris_contig101 207189..207399 +
1622815279.5391786-UTR-P-lacustris_contig101:208731..2090821622815279.5391786-UTR-P-lacustris_contig101:208731..209082Pleurocladia lacustris SAG_25_93UTRP-lacustris_contig101 208732..209082 +
1693493447.195377-UTR-P-lacustris_contig101:208731..2090821693493447.195377-UTR-P-lacustris_contig101:208731..209082Pleurocladia lacustris SAG_25_93UTRP-lacustris_contig101 208732..209082 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815279.5091867-CDS-P-lacustris_contig101:207399..2075051622815279.5091867-CDS-P-lacustris_contig101:207399..207505Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig101 207400..207505 +
1693493447.1636963-CDS-P-lacustris_contig101:207399..2075051693493447.1636963-CDS-P-lacustris_contig101:207399..207505Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig101 207400..207505 +
1622815279.5220773-CDS-P-lacustris_contig101:207999..2081271622815279.5220773-CDS-P-lacustris_contig101:207999..208127Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig101 208000..208127 +
1693493447.1777337-CDS-P-lacustris_contig101:207999..2081271693493447.1777337-CDS-P-lacustris_contig101:207999..208127Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig101 208000..208127 +
1622815279.5305634-CDS-P-lacustris_contig101:208482..2087311622815279.5305634-CDS-P-lacustris_contig101:208482..208731Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig101 208483..208731 +
1693493447.1867533-CDS-P-lacustris_contig101:208482..2087311693493447.1867533-CDS-P-lacustris_contig101:208482..208731Pleurocladia lacustris SAG_25_93CDSP-lacustris_contig101 208483..208731 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-lacustris_contig101.199.1

>prot_P-lacustris_contig101.199.1 ID=prot_P-lacustris_contig101.199.1|Name=mRNA_P-lacustris_contig101.199.1|organism=Pleurocladia lacustris SAG_25_93|type=polypeptide|length=161bp
MICTYLVGINALSFMMHAEDKASSVGWLPFVGRIPEALLHQVELAGGSPG
SFLGQRLFRHKVSKLSYQRGFSQVLIVQVAASIVWGYFKGATWALAAAGL
APAVMFALTARHMRTFKYRQHRRDNGSYGDGRHQPWQRPVDVSAPAGRRR
NTRSRRGAYK*
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mRNA from alignment at P-lacustris_contig101:207189..209082+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-lacustris_contig101.199.1 ID=mRNA_P-lacustris_contig101.199.1|Name=mRNA_P-lacustris_contig101.199.1|organism=Pleurocladia lacustris SAG_25_93|type=mRNA|length=1894bp|location=Sequence derived from alignment at P-lacustris_contig101:207189..209082+ (Pleurocladia lacustris SAG_25_93)
GGCCGAACATGGGCAGGATGCACACTACTGCTGTACAAACCTGGACGTGA
GCTACAAGGCAGACGCTTCTGTGTGCAGCAGCAACTCGGCCATTTCCTAC
CCTCGAACCATCTCAACATATCGGATTCTACTTACAGTATTGACGCTTGC
AAGAGAGAGGGAAAGGGAGGTTGGTGTGCATCTACTTCAACGGGCGCTGC
TGCAACGAGAGATGATTTGCACCTACCTCGTTGGCATCAATGCTCTCTCT
TTCATGATGCACGCCGAGGATAAGGCCTCATCCGTCGGCTGGTTGCCATT
TGTCGGTCGAATCCCAGGTGCCTCTGCCATGCCTCTCCAACGCACGGACT
TTTGTGTTTTGTATTTCGTCCGCCCCATGCATACTGACTGTGCAGCACAG
CGGGCTTTACGTTGTTGCTGTAGCTCTGTTGCTGCCTGTGGCTACAAGAT
GGCTTAAATATTCCGTCGTTGTGGTTGTTGTTTTTGGGCGTGTTTGTTGT
TATTGTGTGGACTGTAAAACGTTCATGATTTTCGTCGGTTTTCCAGCAGC
GATCAATTCTTGTCCGGCCCACGTGTCAAGCTTCGCAACGTCGGCGGACC
GCCATCTGTCACCACTATCCGTCTCACCTATACAATTCCTTACCAGTATC
TCCACCCCTTTGCTTATGACAAGGATGACAAGTGATTGCATGCCCTCCGC
GATACGGAAGCGACACGGGGCGAAATCTTACGATACGCAAAACTTCGTTA
ACTTGATTCTTACAGCAGTATCCGTCTACTTCTCTAACTCGAAGTTTACC
CCTCGCTGCAGAGGCCCTGCTGCATCAGGTCGAGCTCGCGGGAGGTTCCC
CAGGGTCCTTTCTGGGCCAGCGTTTATTCCGACACAAGGTCTCCAAGCTC
TCTTACCAGAGAGGGTTTTCCCAGGTGCTCATCGTACAGGTGAGACGCAG
CAAGGACGGAAATATCGTGTGAGCCATTATCCTTCTTGTAAGACAACAGC
GGTCGCGCGAAAGCCAATTACTAACGACGCCCGGGTTGTGGGCACGCCTT
CCACTTCATAAGCGGACAACGGTCGCAGCGCAGTGCAATTGGTGGTGGAT
CGGACGTTGCCCTTGCGGCTACGTGCTGCTCGTCTTTGAAGAATGGCCTT
CTTTGATGGTTTTCGTTTTTGACAGCGGGCTGAGACTCTCGGCAGGTACA
TGTGTGCAACCTTGCCTGTCAACAAACTTGTACTCGGTCCTCCCGTTTCG
CATCCTGCCACACCCTGGTGTTGTGTTGTTTCGGGGTTGGCCAGGTTGCT
GCGAGCATCGTCTGGGGCTACTTCAAAGGCGCAACCTGGGCCCTGGCGGC
GGCGGGGCTCGCGCCGGCGGTGATGTTTGCCCTCACGGCCAGGCATATGA
GGACTTTCAAATATCGACAACACCGTCGGGACAACGGCAGTTACGGGGAC
GGGCGGCATCAGCCGTGGCAAAGACCTGTCGACGTTTCCGCCCCTGCCGG
TAGACGCAGAAATACTCGTTCCAGGAGGGGGGCGTACAAGTAGAAGGACA
GTCAGTGCTTGGTTCCGAAGTCGTATTTTAGCCTCAGACCATCGAAGGGG
GACAGATGCTGCTCTGGATATTGAAATCGTTGGAGGTTGTCGCGTGCGGC
ATCCCGCTAGGCGAGGAGCGCAGGGTTCTCACGTTAACGATTACCCCTTG
AACCCTGAGGGATGGCTTTGCAAGGAGCGTTGCCTACTACTCGATAAGAG
TTTATGGAACACTTGGTGTGTGCATGGCGAGACAACAATTGGTCAGGCAA
TGAGAACATCAAGAAAAGCAACAGAAACTGACTCAAACGAAGAGTGGACG
TTGCGGCTATAAATAGAGGCTATATTTCAAGCCGTAGCTCGTCG
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Coding sequence (CDS) from alignment at P-lacustris_contig101:207189..209082+

>mRNA_P-lacustris_contig101.199.1 ID=mRNA_P-lacustris_contig101.199.1|Name=mRNA_P-lacustris_contig101.199.1|organism=Pleurocladia lacustris SAG_25_93|type=CDS|length=966bp|location=Sequence derived from alignment at P-lacustris_contig101:207189..209082+ (Pleurocladia lacustris SAG_25_93)
ATGATTTGCACCTACCTCGTTGGCATCAATGCTCTCTCTTTCATGATGCA
CGCCGAGGATAAGGCCTCATCCGTCGGCTGGTTGCCATTTGTCGGTCGAA
TCCCAGATGATTTGCACCTACCTCGTTGGCATCAATGCTCTCTCTTTCAT
GATGCACGCCGAGGATAAGGCCTCATCCGTCGGCTGGTTGCCATTTGTCG
GTCGAATCCCAGAGGCCCTGCTGCATCAGGTCGAGCTCGCGGGAGGTTCC
CCAGGGTCCTTTCTGGGCCAGCGTTTATTCCGACACAAGGTCTCCAAGCT
CTCTTACCAGAGAGGGTTTTCCCAGGTGCTCATCGTACAGAGGCCCTGCT
GCATCAGGTCGAGCTCGCGGGAGGTTCCCCAGGGTCCTTTCTGGGCCAGC
GTTTATTCCGACACAAGGTCTCCAAGCTCTCTTACCAGAGAGGGTTTTCC
CAGGTGCTCATCGTACAGGTTGCTGCGAGCATCGTCTGGGGCTACTTCAA
AGGCGCAACCTGGGCCCTGGCGGCGGCGGGGCTCGCGCCGGCGGTGATGT
TTGCCCTCACGGCCAGGCATATGAGGACTTTCAAATATCGACAACACCGT
CGGGACAACGGCAGTTACGGGGACGGGCGGCATCAGCCGTGGCAAAGACC
TGTCGACGTTTCCGCCCCTGCCGGTAGACGCAGAAATACTCGTTCCAGGA
GGGGGGCGTACAAGTAGGTTGCTGCGAGCATCGTCTGGGGCTACTTCAAA
GGCGCAACCTGGGCCCTGGCGGCGGCGGGGCTCGCGCCGGCGGTGATGTT
TGCCCTCACGGCCAGGCATATGAGGACTTTCAAATATCGACAACACCGTC
GGGACAACGGCAGTTACGGGGACGGGCGGCATCAGCCGTGGCAAAGACCT
GTCGACGTTTCCGCCCCTGCCGGTAGACGCAGAAATACTCGTTCCAGGAG
GGGGGCGTACAAGTAG
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