mRNA_P-wetherbeei_contig15244.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig15244.1.1
Unique NamemRNA_P-wetherbeei_contig15244.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: UPI00190C16B5 (RNA pseudouridine synthase n=14 Tax=Bradyrhizobium TaxID=374 RepID=UPI00190C16B5)

HSP 1 Score: 313 bits (801), Expect = 8.980e-107
Identity = 141/156 (90.38%), Postives = 149/156 (95.51%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLDKDTSGCLVLGRHRKATASLGLLFKHGKI KTYW VVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKA+TNWKV GRG+GL W+ +EPVTGRTHQLRVH+SA+GWPIFGDNIYG GPRFGEPRLHLHSREIV+PISRNKEP+RV APAP
Sbjct:   63 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIGKTYWTVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAITNWKVMGRGDGLTWLAMEPVTGRTHQLRVHSSATGWPIFGDNIYGNGPRFGEPRLHLHSREIVVPISRNKEPIRVVAPAP 218
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: A0A023XTW9_BRAJP (PseudoU_synth_2 domain-containing protein n=2 Tax=Bradyrhizobium japonicum TaxID=375 RepID=A0A023XTW9_BRAJP)

HSP 1 Score: 310 bits (795), Expect = 7.890e-106
Identity = 139/156 (89.10%), Postives = 149/156 (95.51%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLDKDTSGCLVLGRHRKATASLGLLFKHGKI KTYW VVEGGPA+DEGTIDMPLGRLNAERGWWQKPDPEGQKA+TNWKV GRG+GL W+ +EPVTGRTHQLRVH++A+GWPIFGDNIYG GPRFGEPRLHLHSREIV+PISRNKEP+RV APAP
Sbjct:   65 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIGKTYWTVVEGGPAQDEGTIDMPLGRLNAERGWWQKPDPEGQKAITNWKVMGRGDGLTWLAMEPVTGRTHQLRVHSAATGWPIFGDNIYGNGPRFGEPRLHLHSREIVVPISRNKEPIRVVAPAP 220
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: A0A533J3H5_9BRAD (RNA pseudouridine synthase (Fragment) n=1 Tax=Bradyrhizobium icense TaxID=1274631 RepID=A0A533J3H5_9BRAD)

HSP 1 Score: 309 bits (792), Expect = 1.060e-105
Identity = 140/156 (89.74%), Postives = 147/156 (94.23%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLDKDTSGCLVLGRHRKATASLGLLFKHGK+EKTYW VVEGGPAEDEG IDMPLGRLNAERGWWQKPDPEGQKA+TNWKV GRG+GLAW+ +EPVTGRTHQLRVH +ASGWPIFGDNIYG GPRFGEPRLHLHSR I +PISRNKEPVRV APAP
Sbjct:   48 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKVEKTYWTVVEGGPAEDEGVIDMPLGRLNAERGWWQKPDPEGQKAITNWKVLGRGDGLAWLAMEPVTGRTHQLRVHAAASGWPIFGDNIYGNGPRFGEPRLHLHSRAIGVPISRNKEPVRVVAPAP 203
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: A0A1M5JEU7_9BRAD (tRNA pseudouridine32 synthase / 23S rRNA pseudouridine746 synthase n=13 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A1M5JEU7_9BRAD)

HSP 1 Score: 310 bits (793), Expect = 1.890e-105
Identity = 142/156 (91.03%), Postives = 147/156 (94.23%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLD+DTSGCLVLGRHRKATASLGLLFKHGKI KTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKV GRG+GL W+ LEPVTGRTHQLRVH +A GWPIFGDNIYG GPRFGEP LHLHSREIVIPISRNK+PVRV APAP
Sbjct:   70 HRLDRDTSGCLVLGRHRKATASLGLLFKHGKISKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVLGRGDGLTWLALEPVTGRTHQLRVHCAAMGWPIFGDNIYGNGPRFGEPHLHLHSREIVIPISRNKDPVRVVAPAP 225
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: A0A7W6T296_9BRAD (tRNA pseudouridine32 synthase/23S rRNA pseudouridine746 synthase n=85 Tax=Bradyrhizobiaceae TaxID=41294 RepID=A0A7W6T296_9BRAD)

HSP 1 Score: 309 bits (791), Expect = 2.440e-105
Identity = 138/156 (88.46%), Postives = 148/156 (94.87%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLDKDTSGCLVLGRHRKATASLGLLFKHGKI KTYW VVEGGPAEDEGT+DMPLGRLNAERGWWQKPDPEGQKA+TNWKV GRG+G  W+ +EPVTGRTHQLRVH++A+GWPIFGDNIYG GPRFGEP+LHLHSREIV+PISRNKEPVRV APAP
Sbjct:   57 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIGKTYWTVVEGGPAEDEGTVDMPLGRLNAERGWWQKPDPEGQKAITNWKVMGRGDGFTWLAMEPVTGRTHQLRVHSAATGWPIFGDNIYGNGPRFGEPKLHLHSREIVVPISRNKEPVRVVAPAP 212
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: A0A2U3PQW4_9BRAD (Putative enzyme n=107 Tax=Hyphomicrobiales TaxID=356 RepID=A0A2U3PQW4_9BRAD)

HSP 1 Score: 309 bits (791), Expect = 3.000e-105
Identity = 138/156 (88.46%), Postives = 148/156 (94.87%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLDKDTSGCLVLGRHRKATASLGLLFKHGKI KTYW VVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKA+TNW+V GRG+G  W+ +EPVTGRTHQLRVH++A+GWPIFGDNIYG GPRFGEPRLHLHSREIV+PISRNKEP+RV APAP
Sbjct:   63 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIGKTYWTVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAITNWRVMGRGDGFTWLAMEPVTGRTHQLRVHSAATGWPIFGDNIYGNGPRFGEPRLHLHSREIVVPISRNKEPIRVVAPAP 218
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: UPI001BA4B237 (RNA pseudouridine synthase n=2 Tax=Bradyrhizobium TaxID=374 RepID=UPI001BA4B237)

HSP 1 Score: 307 bits (786), Expect = 1.730e-104
Identity = 138/156 (88.46%), Postives = 147/156 (94.23%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLDKDTSGCLVLGRHRKATASLGLLFKHGKI KTYW VVEGGP EDEG IDMPLGRLNAERGWWQKPDPEGQKA+TNWKV GRG+GL W+ +EPVTGRTHQLRVH++A+GWPIFGDNIYG GPRFGEP+LHLHSREIV+PISRNKEPVRV APAP
Sbjct:   63 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIGKTYWTVVEGGPVEDEGIIDMPLGRLNAERGWWQKPDPEGQKAITNWKVMGRGDGLTWLAMEPVTGRTHQLRVHSAATGWPIFGDNIYGNGPRFGEPKLHLHSREIVVPISRNKEPVRVVAPAP 218
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: UPI000577FE67 (RNA pseudouridine synthase n=1 Tax=Bradyrhizobium sp. CCBAU 15544 TaxID=455353 RepID=UPI000577FE67)

HSP 1 Score: 303 bits (777), Expect = 4.450e-104
Identity = 136/154 (88.31%), Postives = 146/154 (94.81%), Query Frame = 1 
Query:    7 LDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            LD+DTSGCLVLGRHRKATASLGLLFKHGKI KTYW VVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKA+TNWKV GRG+G  W+ +EPVTGRTHQLRVH++A+GWPIFGDNIYG GPRFGEP+LHLHSREIV+PISRNKEPVRV APAP
Sbjct:    1 LDRDTSGCLVLGRHRKATASLGLLFKHGKIGKTYWTVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAITNWKVMGRGDGFTWLAMEPVTGRTHQLRVHSAATGWPIFGDNIYGNGPRFGEPKLHLHSREIVVPISRNKEPVRVVAPAP 154
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: UPI001BA4E1CA (RNA pseudouridine synthase n=1 Tax=Bradyrhizobium lablabi TaxID=722472 RepID=UPI001BA4E1CA)

HSP 1 Score: 306 bits (783), Expect = 6.300e-104
Identity = 139/156 (89.10%), Postives = 145/156 (92.95%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLDKDTSGCLVLGRHRKATASLGLLFKHGK+ KTYWAVVEGGPAEDEGT+DMPLGRLNAERGWWQKPDPEGQKAVTNWKV GRG G+AW+ +EPVTGRTHQLRVHTSA GWPI GDNIYG GPRFGEPRLHLHSREI +PISRNK PV V APAP
Sbjct:   70 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKVSKTYWAVVEGGPAEDEGTVDMPLGRLNAERGWWQKPDPEGQKAVTNWKVLGRGGGVAWLAMEPVTGRTHQLRVHTSAMGWPIVGDNIYGNGPRFGEPRLHLHSREIGVPISRNKPPVHVVAPAP 225
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BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Match: A0A4Q8QVV1_9BRAD (RNA pseudouridine synthase n=11 Tax=Bradyrhizobium TaxID=374 RepID=A0A4Q8QVV1_9BRAD)

HSP 1 Score: 305 bits (780), Expect = 8.470e-104
Identity = 137/156 (87.82%), Postives = 147/156 (94.23%), Query Frame = 1 
Query:    1 HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGTIDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRTHQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVRVAAPAP 468
            HRLD+DTSGCLVLGRHRKATASLGLLFKHGKI KTYWAVVEGGPAE++GTID+PLGR NAERGWWQKPDPEGQKAVTNWK+ GRGNGLAWV +EPVTGRTHQLRVH SA GWPI GDNIYG GPRFGEPRLHLHSRE++IPIS+NK+PVRV APAP
Sbjct:   48 HRLDRDTSGCLVLGRHRKATASLGLLFKHGKISKTYWAVVEGGPAEEQGTIDLPLGRKNAERGWWQKPDPEGQKAVTNWKILGRGNGLAWVAMEPVTGRTHQLRVHASAMGWPIVGDNIYGNGPRFGEPRLHLHSRELMIPISKNKDPVRVTAPAP 203
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig15244.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI00190C16B58.980e-10790.38RNA pseudouridine synthase n=14 Tax=Bradyrhizobium... [more]
A0A023XTW9_BRAJP7.890e-10689.10PseudoU_synth_2 domain-containing protein n=2 Tax=... [more]
A0A533J3H5_9BRAD1.060e-10589.74RNA pseudouridine synthase (Fragment) n=1 Tax=Brad... [more]
A0A1M5JEU7_9BRAD1.890e-10591.03tRNA pseudouridine32 synthase / 23S rRNA pseudouri... [more]
A0A7W6T296_9BRAD2.440e-10588.46tRNA pseudouridine32 synthase/23S rRNA pseudouridi... [more]
A0A2U3PQW4_9BRAD3.000e-10588.46Putative enzyme n=107 Tax=Hyphomicrobiales TaxID=3... [more]
UPI001BA4B2371.730e-10488.46RNA pseudouridine synthase n=2 Tax=Bradyrhizobium ... [more]
UPI000577FE674.450e-10488.31RNA pseudouridine synthase n=1 Tax=Bradyrhizobium ... [more]
UPI001BA4E1CA6.300e-10489.10RNA pseudouridine synthase n=1 Tax=Bradyrhizobium ... [more]
A0A4Q8QVV1_9BRAD8.470e-10487.82RNA pseudouridine synthase n=11 Tax=Bradyrhizobium... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig15244contigP-wetherbeei_contig15244:122..589 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1037409.BJ6T_04430
Preferred nameMA20_43745
PFAMsPseudoU_synth_2
Model size468
Max annot lvl28211|Alphaproteobacteria
KEGG koko:K06177
Hectar predicted targeting categoryother localisation
Exons1
Evalue5.99e-107
EggNOG OGsCOG0564@1|root,COG0564@2|Bacteria,1MVJ5@1224|Proteobacteria,2TSCG@28211|Alphaproteobacteria,3JRTQ@41294|Bradyrhizobiaceae
EC5.4.99.28,5.4.99.29
DescriptionPseudouridine synthase
Cds size468
COG categoryJ
BRITEko00000,ko01000,ko03009,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186049.4360902-CDS-P-wetherbeei_contig15244:121..5891683186049.4360902-CDS-P-wetherbeei_contig15244:121..589Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig15244 122..589 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig15244.1.1prot_P-wetherbeei_contig15244.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig15244 122..589 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig15244.1.1

>prot_P-wetherbeei_contig15244.1.1 ID=prot_P-wetherbeei_contig15244.1.1|Name=mRNA_P-wetherbeei_contig15244.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=156bp
HRLDKDTSGCLVLGRHRKATASLGLLFKHGKIEKTYWAVVEGGPAEDEGT
IDMPLGRLNAERGWWQKPDPEGQKAVTNWKVRGRGNGLAWVELEPVTGRT
HQLRVHTSASGWPIFGDNIYGTGPRFGEPRLHLHSREIVIPISRNKEPVR
VAAPAP
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mRNA from alignment at P-wetherbeei_contig15244:122..589+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig15244.1.1 ID=mRNA_P-wetherbeei_contig15244.1.1|Name=mRNA_P-wetherbeei_contig15244.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=468bp|location=Sequence derived from alignment at P-wetherbeei_contig15244:122..589+ (Phaeothamnion wetherbeei SAG_119_79)
CACCGGCTCGACAAGGATACGTCGGGCTGTCTCGTGCTGGGACGCCACCG
CAAGGCCACCGCCTCGCTCGGCCTGTTGTTCAAGCACGGCAAAATCGAGA
AAACCTATTGGGCCGTGGTCGAAGGCGGACCTGCCGAAGATGAAGGCACC
ATCGACATGCCGCTCGGCCGACTGAACGCCGAGCGCGGCTGGTGGCAGAA
GCCGGACCCCGAGGGGCAAAAGGCCGTCACCAACTGGAAGGTGCGCGGCC
GCGGCAATGGGCTCGCCTGGGTCGAGCTGGAACCTGTCACCGGCCGCACC
CATCAACTGCGCGTGCATACATCGGCGAGCGGCTGGCCAATCTTCGGCGA
CAACATCTACGGCACCGGCCCGCGCTTCGGCGAGCCGCGCCTGCATCTGC
ACTCCCGCGAGATCGTGATTCCGATTTCGCGCAACAAGGAGCCGGTGCGT
GTGGCGGCGCCGGCGCCG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig15244:122..589+

>mRNA_P-wetherbeei_contig15244.1.1 ID=mRNA_P-wetherbeei_contig15244.1.1|Name=mRNA_P-wetherbeei_contig15244.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=468bp|location=Sequence derived from alignment at P-wetherbeei_contig15244:122..589+ (Phaeothamnion wetherbeei SAG_119_79)
CACCGGCTCGACAAGGATACGTCGGGCTGTCTCGTGCTGGGACGCCACCG
CAAGGCCACCGCCTCGCTCGGCCTGTTGTTCAAGCACGGCAAAATCGAGA
AAACCTATTGGGCCGTGGTCGAAGGCGGACCTGCCGAAGATGAAGGCACC
ATCGACATGCCGCTCGGCCGACTGAACGCCGAGCGCGGCTGGTGGCAGAA
GCCGGACCCCGAGGGGCAAAAGGCCGTCACCAACTGGAAGGTGCGCGGCC
GCGGCAATGGGCTCGCCTGGGTCGAGCTGGAACCTGTCACCGGCCGCACC
CATCAACTGCGCGTGCATACATCGGCGAGCGGCTGGCCAATCTTCGGCGA
CAACATCTACGGCACCGGCCCGCGCTTCGGCGAGCCGCGCCTGCATCTGC
ACTCCCGCGAGATCGTGATTCCGATTTCGCGCAACAAGGAGCCGGTGCGT
GTGGCGGCGCCGGCGCCG
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