mRNA_P-wetherbeei_contig9959.4.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig9959.4.1
Unique NamemRNA_P-wetherbeei_contig9959.4.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: D7FKR2_ECTSI (Glutamyl-tRNA(Gln) and/or aspartyl-tRNA(Asn) amidotransferase, C subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FKR2_ECTSI)

HSP 1 Score: 92.4 bits (228), Expect = 1.910e-21
Identity = 47/105 (44.76%), Postives = 70/105 (66.67%), Query Frame = 1
Query:   13 EITEELIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGEE 327
            +IT +L+RKTA LAQ++V+D+EV  L PR+  FLGFVE++  VA++    +D A   +        G  FL+ D+ ++F+    I+ NMP+EEG YLRVP+VGE+
Sbjct:   67 DITPDLVRKTAMLAQIQVSDEEVDVLAPRMEEFLGFVETMKEVAEA----SDGAFATLN------CGSDFLREDSADRFSNVAAIMENMPIEEGNYLRVPKVGED 161          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: A0A836CJS8_9STRA (C subunit of glutamyl-tRNA and/or aspartyl-tRNA amidotransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CJS8_9STRA)

HSP 1 Score: 84.7 bits (208), Expect = 1.520e-18
Identity = 50/100 (50.00%), Postives = 62/100 (62.00%), Query Frame = 1
Query:   28 LIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGEE 327
            LI KTA LAQLEV+D E ++L+PRVRSFLGFV +++       G AD     +G    GL    FL++D   KF     I ANMP+ EG YLRVP+VG E
Sbjct:   57 LILKTARLAQLEVSDAEAEQLVPRVRSFLGFVAAMDEA-----GAADGDAPPMGDSREGLGEGGFLRSDGAVKFENADAIAANMPLREGEYLRVPKVGAE 151          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: W7TR28_9STRA (Glutamyl-trna amidotransferase subunit c n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TR28_9STRA)

HSP 1 Score: 78.6 bits (192), Expect = 3.330e-16
Identity = 42/107 (39.25%), Postives = 60/107 (56.07%), Query Frame = 1
Query:    7 RPEITEELIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGEE 327
            +PE+T ELI+ TA LAQL   + E+Q ++PR ++FL FV+ +  V DS    A  +M  V          + L+ D+P  F     I  NM  +E  +LRVP+VGEE
Sbjct:   48 QPEVTPELIKHTATLAQLHFDEDEIQNMIPRFQAFLSFVDRMQDVPDSAAVSAPDSMPTV---------QEVLRPDSPSIFPNQDAIFTNMAEQEDAFLRVPKVGEE 145          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: A0A0G4EZ16_VITBC (Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial n=1 Tax=Vitrella brassicaformis (strain CCMP3155) TaxID=1169540 RepID=A0A0G4EZ16_VITBC)

HSP 1 Score: 67.0 bits (162), Expect = 2.180e-11
Identity = 40/105 (38.10%), Postives = 61/105 (58.10%), Query Frame = 1
Query:   16 ITEELIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAMEAVGVVV--GGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGE 324
            + E L+RKTAALA L++TD EV  ++ + + FL FV+ + A+        D++   + V+     +     L+ DT + F     I+ANMP EE G+LR+PRVGE
Sbjct:   87 VDEALVRKTAALAHLDLTDDEVSEMVHQFKDFLRFVDDMKAI--------DTSQLDIDVMSPRASVPYRDPLREDTVQPFDNREAIVANMPEEEDGFLRIPRVGE 183          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: A0A0G4IA18_9ALVE (Uncharacterized protein n=1 Tax=Chromera velia CCMP2878 TaxID=1169474 RepID=A0A0G4IA18_9ALVE)

HSP 1 Score: 63.9 bits (154), Expect = 2.790e-10
Identity = 41/104 (39.42%), Postives = 55/104 (52.88%), Query Frame = 1
Query:   13 EITEELIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGE 324
            E+T EL++ TA LA L V++ E+  L+P V  FL FV+ +N V D    G      A       L     L+ DTP +F   G I  NMP EE   L+VP++GE
Sbjct:   78 EVTAELLKNTAKLAGLSVSENEITDLIPEVSKFLKFVDQMNQV-DVDAPGVQELPRAD------LPWSDPLRPDTPNRFNNVGAIWDNMPEEEDEMLKVPQLGE 174          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: A0A6H5KRS3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KRS3_9PHAE)

HSP 1 Score: 60.8 bits (146), Expect = 3.970e-10
Identity = 30/76 (39.47%), Postives = 46/76 (60.53%), Query Frame = 1
Query:  100 VRSFLGFVESINAVADSGGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGEE 327
            +  FLGFVE++  VA++    +D A   +        G  FL+ D+ E+F+    ++ NMP+EEG YLRVP+VGE+
Sbjct:    1 MEEFLGFVETMEEVAEA----SDGAFATLN------CGSDFLREDSAERFSNVAAVMENMPIEEGNYLRVPKVGED 66          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: A0A7S1X181_9CHLO (Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial n=1 Tax=Tetraselmis chuii TaxID=63592 RepID=A0A7S1X181_9CHLO)

HSP 1 Score: 52.4 bits (124), Expect = 6.680e-6
Identity = 28/100 (28.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:   31 IRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGEEA 330
            +R+ A +AQ+ +TD+EV+   P++ S + +   +  V  +G    + ++ A+G        +  L+ D P  F    D+L  +P +EG Y++VP++  EA
Sbjct:   87 LRELAKMAQIGITDEEVEEWTPQIGSIVEWFGELGKVDVTG---VEPSLRAMGE-------ESTLREDVPVSFPNKEDLLKQVPDKEGVYVKVPKISTEA 176          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: A0A2V3J5X7_9FLOR (Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3J5X7_9FLOR)

HSP 1 Score: 49.3 bits (116), Expect = 2.930e-5
Identity = 26/108 (24.07%), Postives = 55/108 (50.93%), Query Frame = 1
Query:   13 EITEELIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGEEASD 336
            EI+++ ++  A LAQL ++++EV +++P ++  + F ++ + V   G     SA +   V          ++ D P +F     ++  +P  E  Y+RVP++    +D
Sbjct:    8 EISKQEVQNIAKLAQLRLSEEEVNKVVPEIKKIINFFDTFSEVDVEGIEPMTSAQDVHNV----------MREDEPSRFHNVDGMMQEVPCSEREYIRVPKLSSNPTD 105          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: A0A2E1X4A5_9BACT (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit C n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E1X4A5_9BACT)

HSP 1 Score: 49.7 bits (117), Expect = 3.200e-5
Identity = 37/106 (34.91%), Postives = 54/106 (50.94%), Query Frame = 1
Query:    7 RPEITEELIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAME--AVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRV 318
            RP +  EL+RKTA LA+LEV+D+EV  ++  +   L  VE++ +V       AD +++  A  VVV  L  D+ L+  +P        I  N P    G    PRV
Sbjct:   24 RPVVDSELVRKTARLARLEVSDEEVPAMVHHMERILDLVETLRSVEIP----ADLSIDDNARTVVVTQLRADESLEESSPGGPRDQESIGKNAPDWRDGTFVTPRV 125          
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Match: A0A2E8HG09_9BACT (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C n=2 Tax=root TaxID=1 RepID=A0A2E8HG09_9BACT)

HSP 1 Score: 47.8 bits (112), Expect = 9.340e-5
Identity = 33/103 (32.04%), Postives = 52/103 (50.49%), Query Frame = 1
Query:   16 ITEELIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADSGGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGYLRVPRVGE 324
            +T E +++ AALA+LE T  E +RL   + S L ++E +N + D+ G    S +  V             + D PE+FA   ++ A  P    GY +VPR+ E
Sbjct:    3 VTVEDVKRIAALARLEFTADEEERLTGELNSILHYMEKLNGL-DTDGVEPTSHVAPVA---------NAFREDEPEEFAHVEELRAQAPEMVEGYYKVPRIIE 95          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig9959.4.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
D7FKR2_ECTSI1.910e-2144.76Glutamyl-tRNA(Gln) and/or aspartyl-tRNA(Asn) amido... [more]
A0A836CJS8_9STRA1.520e-1850.00C subunit of glutamyl-tRNA and/or aspartyl-tRNA am... [more]
W7TR28_9STRA3.330e-1639.25Glutamyl-trna amidotransferase subunit c n=2 Tax=N... [more]
A0A0G4EZ16_VITBC2.180e-1138.10Glutamyl-tRNA(Gln) amidotransferase subunit C, mit... [more]
A0A0G4IA18_9ALVE2.790e-1039.42Uncharacterized protein n=1 Tax=Chromera velia CCM... [more]
A0A6H5KRS3_9PHAE3.970e-1039.47Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S1X181_9CHLO6.680e-628.00Glutamyl-tRNA(Gln) amidotransferase subunit C, chl... [more]
A0A2V3J5X7_9FLOR2.930e-524.07Glutamyl-tRNA(Gln) amidotransferase subunit C, mit... [more]
A0A2E1X4A5_9BACT3.200e-534.91Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCA... [more]
A0A2E8HG09_9BACT9.340e-532.04Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]
back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig9959contigP-wetherbeei_contig9959:1648..2199 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog670487.Ocepr_1155
Preferred namegatC
PFAMsGlu-tRNAGln
Model size339
Max annot lvl1297|Deinococcus-Thermus
KEGG rclassRC00010
KEGG koko:K02435
KEGG ReactionR03905,R04212
KEGG Pathwayko00970,ko01100,map00970,map01100
Hectar predicted targeting categoryother localisation
Exons2
Evalue2.41e-08
EggNOG OGsCOG0721@1|root,COG0721@2|Bacteria,1WKPW@1297|Deinococcus-Thermus
Ec32 ortholog descriptionglutamyl-tRNA(Gln) and/or aspartyl-tRNA(Asn) amidotransferase, C subunit
Ec32 orthologEc-21_001810.1
EC6.3.5.6,6.3.5.7
DescriptionAllows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
Cds size339
COG categoryJ
BRITEko00000,ko00001,ko01000,ko03029
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683187118.92666-CDS-P-wetherbeei_contig9959:1647..19021683187118.92666-CDS-P-wetherbeei_contig9959:1647..1902Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig9959 1648..1902 -
1683187118.9374907-CDS-P-wetherbeei_contig9959:2115..21991683187118.9374907-CDS-P-wetherbeei_contig9959:2115..2199Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig9959 2116..2199 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig9959.4.1prot_P-wetherbeei_contig9959.4.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig9959 1648..2199 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig9959.4.1

>prot_P-wetherbeei_contig9959.4.1 ID=prot_P-wetherbeei_contig9959.4.1|Name=mRNA_P-wetherbeei_contig9959.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=113bp
MARPEITEELIRKTAALAQLEVTDKEVQRLLPRVRSFLGFVESINAVADS
GGGGADSAMEAVGVVVGGLLGDKFLQTDTPEKFAGDGDILANMPVEEGGY
LRVPRVGEEASD*
back to top

mRNA from alignment at P-wetherbeei_contig9959:1648..2199-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig9959.4.1 ID=mRNA_P-wetherbeei_contig9959.4.1|Name=mRNA_P-wetherbeei_contig9959.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=552bp|location=Sequence derived from alignment at P-wetherbeei_contig9959:1648..2199- (Phaeothamnion wetherbeei SAG_119_79)
ATGGCGCGGCCGGAGATAACAGAGGAGCTGATCCGCAAGACAGCGGCGCT GGCGCAGCTTGAAGTGACTGACAAAGAGGTGCAGGTCTTTCGCACTCTCC CCCTGTTCGAGAACCGTGCTTTACCAGCTTGTGATATGACTCTTTTGATG GTTGTGGTCGAAGAGGAATAATCTGAAGCTTACCCGGCTTTGCGTTTATT CCATTTCCAAAATTTCTTACTTTGGAGCTCTAATCAACAAGAGATGCCCA GATCAAGTTGCTGATTTCCCTTTTGACACCGTGTTCGTCAGGTGGAGCGT CTACTGCCCCGCGTGCGCAGCTTCCTCGGCTTTGTGGAGTCGATCAACGC AGTCGCCGACAGCGGCGGCGGCGGCGCGGACAGCGCGATGGAGGCAGTCG GTGTGGTGGTTGGCGGCCTGCTCGGGGATAAGTTTCTGCAGACGGACACG CCGGAGAAGTTCGCTGGCGACGGCGACATCCTGGCGAACATGCCGGTCGA GGAGGGCGGCTACCTCCGGGTCCCCCGGGTGGGCGAAGAGGCGTCCGACT GA
back to top

Coding sequence (CDS) from alignment at P-wetherbeei_contig9959:1648..2199-

>mRNA_P-wetherbeei_contig9959.4.1 ID=mRNA_P-wetherbeei_contig9959.4.1|Name=mRNA_P-wetherbeei_contig9959.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=339bp|location=Sequence derived from alignment at P-wetherbeei_contig9959:1648..2199- (Phaeothamnion wetherbeei SAG_119_79)
ATGGCGCGGCCGGAGATAACAGAGGAGCTGATCCGCAAGACAGCGGCGCT
GGCGCAGCTTGAAGTGACTGACAAAGAGGTGCAGCGTCTACTGCCCCGCG
TGCGCAGCTTCCTCGGCTTTGTGGAGTCGATCAACGCAGTCGCCGACAGC
GGCGGCGGCGGCGCGGACAGCGCGATGGAGGCAGTCGGTGTGGTGGTTGG
CGGCCTGCTCGGGGATAAGTTTCTGCAGACGGACACGCCGGAGAAGTTCG
CTGGCGACGGCGACATCCTGGCGAACATGCCGGTCGAGGAGGGCGGCTAC
CTCCGGGTCCCCCGGGTGGGCGAAGAGGCGTCCGACTGA
back to top