mRNA_P-wetherbeei_contig7369.3.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig7369.3.1
Unique NamemRNA_P-wetherbeei_contig7369.3.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: A0A351T9B9_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=4 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A351T9B9_9PROT)

HSP 1 Score: 215 bits (548), Expect = 3.670e-69
Identity = 111/163 (68.10%), Postives = 121/163 (74.23%), Query Frame = 1 
Query:    1 MLRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQG----RLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGFSAHSEGAG 477
            MLR+ALY+PDIPQNTGTLLRLGACLGVPVDIIEPCGF+LTDR  +RAGMDYLD A M RHD WP F   L         RL+LLTT A I YT FRF + DTL+LGRESAGVP  V   ADARL+IPML G RSLNVA+  AMVLGE LRQ  GF+     AG
Sbjct:    1 MLRLALYEPDIPQNTGTLLRLGACLGVPVDIIEPCGFLLTDRAFRRAGMDYLDQAMMTRHDGWPEFSATLKSAANGPMRRLVLLTTSAEIPYTEFRFAAHDTLLLGRESAGVPRAVQAAADARLLIPMLPGSRSLNVAVAGAMVLGEALRQCGGFAGQEVKAG 163
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: A0A081BD19_9HYPH (tRNA (cytidine(34)-2'-O)-methyltransferase n=2 Tax=Tepidicaulis marinus TaxID=1333998 RepID=A0A081BD19_9HYPH)

HSP 1 Score: 211 bits (537), Expect = 1.170e-67
Identity = 105/150 (70.00%), Postives = 122/150 (81.33%), Query Frame = 1 
Query:    4 LRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGF 453
            +R+ALYQPDIPQNTGT+LRLGACLGVPVDIIEPCGF  +DRGL+RAGMDY+  A ++RH  W AF+ D  + + RLILLTTKAA  YT FRF  GDTL+LGRESAGVP++VH  ADARL+IP+  G RSLNVA+ AAMVLGE LRQ   F
Sbjct:    1 MRLALYQPDIPQNTGTILRLGACLGVPVDIIEPCGFPFSDRGLRRAGMDYVSHAEIRRHADWQAFQ-DARKPENRLILLTTKAAEPYTGFRFAPGDTLLLGRESAGVPEDVHAAADARLLIPLAPGARSLNVAVAAAMVLGEALRQTQSF 149
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: A0A0P6W4U1_9HYPH (tRNA (cytidine(34)-2'-O)-methyltransferase n=2 Tax=Prosthecomicrobium hirschii TaxID=665126 RepID=A0A0P6W4U1_9HYPH)

HSP 1 Score: 210 bits (534), Expect = 3.690e-67
Identity = 101/152 (66.45%), Postives = 123/152 (80.92%), Query Frame = 1 
Query:    4 LRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGFSA 459
            +R+ LYQPDIPQNTG++LRL ACLGVPVD+IEP GFV+TD+GL+RAG+DYL  A + RHDS+ AF     Q  GRL+L+TT+AA+ YT F +R GD LMLGRESAGVPD VHD A+AR+VIPM+AG RS+NVA+ A M+LGE LRQ  GF A
Sbjct:    1 MRLVLYQPDIPQNTGSILRLAACLGVPVDLIEPAGFVITDKGLRRAGLDYLPAATLTRHDSFAAFETWRRQAGGRLVLVTTRAAMPYTEFAYRPGDLLMLGRESAGVPDAVHDLAEARVVIPMVAGMRSVNVAVAAGMILGEALRQTGGFPA 152
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: A0A1C3RGN7_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Candidatus Terasakiella magnetica TaxID=1867952 RepID=A0A1C3RGN7_9PROT)

HSP 1 Score: 209 bits (533), Expect = 4.450e-67
Identity = 102/152 (67.11%), Postives = 121/152 (79.61%), Query Frame = 1 
Query:    4 LRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGFSA 459
            +R+ALYQPDIPQNTGTLLRL AC+G+ VDIIEPCGFVL D+ ++RAGMDYL    + RH+SW AF  D  ++ GR+ILL+TKAA  YT F +R+ DTL+LGRES GVP EVHDRAD RL+IPM  G RS+NVA+  AMVLGEGLRQ  GF A
Sbjct:    1 MRLALYQPDIPQNTGTLLRLAACMGIGVDIIEPCGFVLDDKRMRRAGMDYLHRVDLIRHNSWEAFLEDKKEKGGRIILLSTKAAHLYTEFTYRADDTLLLGRESQGVPAEVHDRADERLIIPMAEGMRSINVAISGAMVLGEGLRQTGGFPA 152
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: A0A839SW27_9HYPH (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Limibacillus halophilus TaxID=1579333 RepID=A0A839SW27_9HYPH)

HSP 1 Score: 209 bits (531), Expect = 1.250e-66
Identity = 103/155 (66.45%), Postives = 120/155 (77.42%), Query Frame = 1 
Query:    4 LRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGFSAHSE 468
            + + LYQPDIPQNTGTLLRL ACLGVP++IIEPCGF+LTDR L+RAGMDYLD+A++QRH  W  +    + R GRLILLTT    AY  FRF   D +MLGRESAGVP E+H  ADARLVIPM AGRRS+NVA+  AMVLGE LRQ NGF+   +
Sbjct:    3 IELTLYQPDIPQNTGTLLRLAACLGVPMNIIEPCGFLLTDRSLKRAGMDYLDLASVQRHLDWETYN---NSRSGRLILLTTTGNTAYCDFRFHPDDHVMLGRESAGVPQEIHAAADARLVIPMQAGRRSINVAIAGAMVLGEALRQTNGFAVTEQ 154
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: A0A3C0TRL2_9PROT (rRNA methyltransferase (Fragment) n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A3C0TRL2_9PROT)

HSP 1 Score: 206 bits (524), Expect = 4.040e-66
Identity = 97/123 (78.86%), Postives = 112/123 (91.06%), Query Frame = 1 
Query:    1 MLRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIP 369
            MLR+ALYQPDIPQNTGTLLRLGACLGV VDIIEPCGFV+ D+ L+RAG+DYL++AA+ RHDSW AF +DLDQR+GRLILLTT+A ++YTAFRF+ GDTL+LGRESAGVP EVH RADARL IP
Sbjct:    1 MLRLALYQPDIPQNTGTLLRLGACLGVAVDIIEPCGFVMHDKALRRAGLDYLELAAIHRHDSWTAFLVDLDQRRGRLILLTTRAPLSYTAFRFQPGDTLLLGRESAGVPGEVHARADARLTIP 123
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: UPI0019EFED67 (tRNA (Cytidine(34)-2'-O)-methyltransferase n=1 Tax=Proteobacteria bacterium TaxID=1977087 RepID=UPI0019EFED67)

HSP 1 Score: 205 bits (521), Expect = 3.520e-65
Identity = 103/155 (66.45%), Postives = 120/155 (77.42%), Query Frame = 1 
Query:    4 LRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGFSAHSE 468
            +R+ALYQPDIPQNTGT+LRL ACLGV VDIIEPCGFV +D+ L+RAGMDYLD  A  RH SW AFR       GRLILLTT  ++ +T F FR+ D L+LGRESAG PDEVH+ ADAR+ IPM AG RS+NVA+ AA+VLGE LRQ NGF   +E
Sbjct:    1 MRLALYQPDIPQNTGTMLRLAACLGVNVDIIEPCGFVWSDKYLRRAGMDYLDRVAPLRHSSWDAFRERHAAVGGRLILLTTGGSVPHTRFTFRADDCLLLGRESAGAPDEVHEAADARVRIPMTAGMRSINVAMTAAIVLGEALRQTNGFPEENE 155
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: K9GPL7_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Caenispirillum salinarum AK4 TaxID=1238182 RepID=K9GPL7_9PROT)

HSP 1 Score: 203 bits (516), Expect = 2.460e-64
Identity = 107/164 (65.24%), Postives = 125/164 (76.22%), Query Frame = 1 
Query:    4 LRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQR------QGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGFSAHSEGAG 477
            +RIALYQPDIPQNTGT+LRL ACLGV  DIIEPCGF+  DR ++RAGMDY+    M RH SW  F   LD R      +GRL+LLTT+A++ YT F FR+ DTL+LGRESAGVPDEVHD ADAR+V+PM  G RSLNVA+ AAMV+GE LRQ  GF+  S GAG
Sbjct:    1 MRIALYQPDIPQNTGTILRLCACLGVGCDIIEPCGFLFDDRRMRRAGMDYMAQVDMIRHASWRRF---LDSRPTRPGGEGRLVLLTTRASVPYTDFAFRADDTLLLGRESAGVPDEVHDLADARVVVPMAEGARSLNVAVSAAMVVGEALRQTGGFATGS-GAG 160
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: UPI001073C69F (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Terasakiella sp. SH-1 TaxID=2560057 RepID=UPI001073C69F)

HSP 1 Score: 202 bits (515), Expect = 2.700e-64
Identity = 99/152 (65.13%), Postives = 118/152 (77.63%), Query Frame = 1 
Query:    4 LRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGFSA 459
            +R+ALYQPDIPQNTGTLLRL AC+GV VDIIEPCGF+L D+ ++RAGMDYLD   + RH SW  F  D  Q+ GR+ILL+TKAA  YT F ++  DTL+LGRES GVP+EVHD AD RL+IPM  G RS+NVA+  AMVLGE LRQ + F A
Sbjct:    1 MRLALYQPDIPQNTGTLLRLAACMGVGVDIIEPCGFILDDKRMRRAGMDYLDQVDLVRHPSWTTFLDDKRQKGGRIILLSTKAAHLYTEFAYQEDDTLLLGRESQGVPEEVHDTADERLIIPMSEGMRSINVAISGAMVLGEALRQTDCFPA 152
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BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Match: A0A3N1KWX7_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Stella humosa TaxID=94 RepID=A0A3N1KWX7_9PROT)

HSP 1 Score: 202 bits (515), Expect = 3.070e-64
Identity = 102/159 (64.15%), Postives = 123/159 (77.36%), Query Frame = 1 
Query:    4 LRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMDYLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLMLGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNGF-SAHSEGAG 477
            +RIALY+PDIPQN GT++RLGACLGVPVD+IEP GFV  D+ L+RAGMDYLD+AA+ RH SW A+R   ++  GRL+LLT  A + YTAF F  GDTL+LGRESAGVP  VH  ADAR+ +P+  G RSLNVA+ AAM+LGE LRQ  GF +A  E AG
Sbjct:    1 MRIALYEPDIPQNVGTIIRLGACLGVPVDLIEPFGFVWNDQRLRRAGMDYLDLAAVSRHVSWRAYRDRPERASGRLVLLTRHAPLPYTAFAFAPGDTLLLGRESAGVPPAVHGEADARVTVPLCRGLRSLNVAVVAAMILGEALRQTAGFPTADGEPAG 159
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig7369.3.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A351T9B9_9PROT3.670e-6968.10tRNA (cytidine(34)-2'-O)-methyltransferase n=4 Tax... [more]
A0A081BD19_9HYPH1.170e-6770.00tRNA (cytidine(34)-2'-O)-methyltransferase n=2 Tax... [more]
A0A0P6W4U1_9HYPH3.690e-6766.45tRNA (cytidine(34)-2'-O)-methyltransferase n=2 Tax... [more]
A0A1C3RGN7_9PROT4.450e-6767.11tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
A0A839SW27_9HYPH1.250e-6666.45tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
A0A3C0TRL2_9PROT4.040e-6678.86rRNA methyltransferase (Fragment) n=1 Tax=Alphapro... [more]
UPI0019EFED673.520e-6566.45tRNA (Cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
K9GPL7_9PROT2.460e-6465.24tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
UPI001073C69F2.700e-6465.13tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
A0A3N1KWX7_9PROT3.070e-6464.15tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig7369contigP-wetherbeei_contig7369:1455..1934 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog1333998.M2A_2436
Preferred nametrmL
PFAMsSpoU_methylase
Model size480
Max annot lvl28211|Alphaproteobacteria
KEGG koko:K03216
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.92e-68
EggNOG OGsCOG0219@1|root,COG0219@2|Bacteria,1RCY4@1224|Proteobacteria,2U78W@28211|Alphaproteobacteria,4BQCR@82117|unclassified Alphaproteobacteria
EC2.1.1.207
DescriptionMethylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide
Cds size480
COG categoryJ
BRITEko00000,ko01000,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186976.7876456-CDS-P-wetherbeei_contig7369:1454..19341683186976.7876456-CDS-P-wetherbeei_contig7369:1454..1934Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig7369 1455..1934 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig7369.3.1prot_P-wetherbeei_contig7369.3.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig7369 1455..1934 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig7369.3.1

>prot_P-wetherbeei_contig7369.3.1 ID=prot_P-wetherbeei_contig7369.3.1|Name=mRNA_P-wetherbeei_contig7369.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=160bp
MLRIALYQPDIPQNTGTLLRLGACLGVPVDIIEPCGFVLTDRGLQRAGMD
YLDMAAMQRHDSWPAFRLDLDQRQGRLILLTTKAAIAYTAFRFRSGDTLM
LGRESAGVPDEVHDRADARLVIPMLAGRRSLNVALCAAMVLGEGLRQLNG
FSAHSEGAG*
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mRNA from alignment at P-wetherbeei_contig7369:1455..1934+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig7369.3.1 ID=mRNA_P-wetherbeei_contig7369.3.1|Name=mRNA_P-wetherbeei_contig7369.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=480bp|location=Sequence derived from alignment at P-wetherbeei_contig7369:1455..1934+ (Phaeothamnion wetherbeei SAG_119_79)
ATGCTGCGGATCGCCCTGTATCAGCCGGACATCCCTCAGAACACGGGTAC
CCTGTTGCGTCTGGGCGCATGTCTTGGGGTGCCCGTGGATATAATCGAGC
CGTGCGGTTTTGTCCTTACCGACCGGGGTTTGCAGCGCGCGGGCATGGAT
TATCTGGATATGGCGGCAATGCAGCGCCACGATTCGTGGCCGGCGTTTCG
CCTTGATCTGGATCAACGTCAGGGGCGCTTGATCCTGTTAACGACAAAAG
CGGCAATTGCGTACACTGCGTTTCGCTTTCGGTCCGGTGATACGCTAATG
CTGGGCCGTGAAAGTGCAGGCGTGCCGGATGAGGTGCATGATCGCGCCGA
TGCCCGGCTGGTCATACCGATGCTGGCGGGGCGGCGTTCGCTTAATGTAG
CGCTTTGCGCCGCGATGGTGCTGGGTGAGGGGTTGCGTCAGTTAAATGGA
TTTTCAGCGCATTCAGAAGGTGCAGGGTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig7369:1455..1934+

>mRNA_P-wetherbeei_contig7369.3.1 ID=mRNA_P-wetherbeei_contig7369.3.1|Name=mRNA_P-wetherbeei_contig7369.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=480bp|location=Sequence derived from alignment at P-wetherbeei_contig7369:1455..1934+ (Phaeothamnion wetherbeei SAG_119_79)
ATGCTGCGGATCGCCCTGTATCAGCCGGACATCCCTCAGAACACGGGTAC
CCTGTTGCGTCTGGGCGCATGTCTTGGGGTGCCCGTGGATATAATCGAGC
CGTGCGGTTTTGTCCTTACCGACCGGGGTTTGCAGCGCGCGGGCATGGAT
TATCTGGATATGGCGGCAATGCAGCGCCACGATTCGTGGCCGGCGTTTCG
CCTTGATCTGGATCAACGTCAGGGGCGCTTGATCCTGTTAACGACAAAAG
CGGCAATTGCGTACACTGCGTTTCGCTTTCGGTCCGGTGATACGCTAATG
CTGGGCCGTGAAAGTGCAGGCGTGCCGGATGAGGTGCATGATCGCGCCGA
TGCCCGGCTGGTCATACCGATGCTGGCGGGGCGGCGTTCGCTTAATGTAG
CGCTTTGCGCCGCGATGGTGCTGGGTGAGGGGTTGCGTCAGTTAAATGGA
TTTTCAGCGCATTCAGAAGGTGCAGGGTGA
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