mRNA_P-wetherbeei_contig682.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: A0A3G2USG4_SPHYA (Glutamine-dependent NAD(+) synthetase n=5 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A3G2USG4_SPHYA)

HSP 1 Score: 1055 bits (2727), Expect = 0.000e+0
Identity = 533/647 (82.38%), Postives = 571/647 (88.25%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHDA----WPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            MSFYSIY+QGFVRVAACTPKCEVGD  YNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQA+EELAAAS +L P++LVGAPLEREGR YNC VAIHRGRILGVVPKGHLPNYREFYEKRWF SGRD+K A +EIAGQSVPFGMDLIFAAEDLAG +FH ELCEDVW P PPSDFAALA A ILANLSA NI IGKAETRRLLCATQSARCWAA++YSAAGPGESTTDLAWD QACIYELG+LL +TD+FPQDSQMA AD+DVDRL      TGTF EAAIAQ MP DR RRI F FEP  A+ GL + +ARFPYAPADPARLDQDC EAY+IQ+QGL+KRL+ATGIRRLCIGVSG L ST A IVAAKAFD+ GWPRSD+LGFTMP FAT E TKTNA ALM SL +T E IDIKPLA QMLA LQHPF++G+PVYDVTFENVQAGL T+ILFRAANQR AMVLGTGDLSEIA GW TYGVGDQMSHYNVNASVPKTLIQ+LIRWV +S+AFD++T+ TLLS LDTVISPELVP GA GALQS  EKIGPYELQDFT FYLT YGLKPSKIA  A HAW DA    WPPQYP ERRRAYDL +IKSWMEVFL+RFFT+SQFKRSA+PNGPKV
Sbjct:    1 MSFYSIYQQGFVRVAACTPKCEVGDPSYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAVEELAAASRELLPVLLVGAPLEREGRLYNCAVAIHRGRILGVVPKGHLPNYREFYEKRWFASGRDVKGAIMEIAGQSVPFGMDLIFAAEDLAGFVFHTELCEDVWGPAPPSDFAALAGALILANLSASNITIGKAETRRLLCATQSARCWAAYIYSAAGPGESTTDLAWDGQACIYELGALLAETDRFPQDSQMATADIDVDRLRLERLRTGTFNEAAIAQGMPGDRFRRIGFRFEPPTADLGLHRKMARFPYAPADPARLDQDCYEAYNIQVQGLIKRLQATGIRRLCIGVSGGLDSTHALIVAAKAFDRLGWPRSDILGFTMPGFATGESTKTNAWALMSSLGITAEEIDIKPLALQMLAVLQHPFAQGDPVYDVTFENVQAGLRTDILFRAANQRSAMVLGTGDLSEIALGWSTYGVGDQMSHYNVNASVPKTLIQYLIRWVTQSDAFDEETSRTLLSILDTVISPELVPAGAGGALQSTEEKIGPYELQDFTLFYLTRYGLKPSKIAFLALHAWRDAATGDWPPQYPQERRRAYDLATIKSWMEVFLYRFFTVSQFKRSASPNGPKV 647          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: A0A5S9PGZ2_9HYPH (Glutamine-dependent NAD(+) synthetase n=11 Tax=Proteobacteria TaxID=1224 RepID=A0A5S9PGZ2_9HYPH)

HSP 1 Score: 1054 bits (2725), Expect = 0.000e+0
Identity = 532/647 (82.23%), Postives = 568/647 (87.79%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHDA----WPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            MSFYSIY+QGFVRVAACTPKCEV D  YN+AETLALARDGARQGVALMVFPELGL  YAIDDLLGQAALLQRVEQAIEE+AAAS +LSP++L+GAPL REGR YNC VA+HRGRILGVVPKGHLPNYREFYEKRWF SGR+LK ATIEIAGQSVPFGMD+I AAEDL G +FH+ELCEDVW P PPSDFAALA A IL NLSA NI +GKAETRRLLCATQSARCWAA++YSAAGPGESTTDLAWD QACIYELG LL +T++FPQDSQMAIADVDVDRL      TGTF EAAIAQ MP DR RRI F FEP+M + GLR+AVARFPYA ADPARLDQDC EAYSIQ+QGLMKRL+ATGIRR+CIGVSG L ST A IVAAKAFD+ GWPRSD+LGFTMP FAT E TK NA  LM SL VT E IDIKPLAQQMLA+LQHPF++GEPVYDVTFENVQAGL T+ILFRAANQRGAMVLGTGDLSEIA GW TYGVGD MSHYNVNASVPKTLIQHLIRWVA+S AFD+ T  TLLS LDTVISPELVP G  GALQS  EKIGPYELQDFT FYLT YGLKPSKIA  AFHAW DA    WPPQYP ERRRAYDLP+I+SWMEVFL+RFFTISQFKRSA+PNGPKV
Sbjct:    1 MSFYSIYQQGFVRVAACTPKCEVADPSYNVAETLALARDGARQGVALMVFPELGLCGYAIDDLLGQAALLQRVEQAIEEVAAASRELSPVLLIGAPLVREGRLYNCAVAVHRGRILGVVPKGHLPNYREFYEKRWFASGRNLKGATIEIAGQSVPFGMDMILAAEDLPGFVFHVELCEDVWGPAPPSDFAALAGALILTNLSASNITVGKAETRRLLCATQSARCWAAYIYSAAGPGESTTDLAWDGQACIYELGQLLAETERFPQDSQMAIADVDVDRLRLERLRTGTFNEAAIAQAMPEDRFRRIGFRFEPSMTDLGLRRAVARFPYASADPARLDQDCYEAYSIQVQGLMKRLQATGIRRVCIGVSGGLDSTHALIVAAKAFDRLGWPRSDILGFTMPGFATGEATKANAWTLMRSLGVTAEEIDIKPLAQQMLASLQHPFAQGEPVYDVTFENVQAGLRTDILFRAANQRGAMVLGTGDLSEIALGWSTYGVGDHMSHYNVNASVPKTLIQHLIRWVAQSAAFDEATNGTLLSILDTVISPELVPAGEGGALQSTEEKIGPYELQDFTLFYLTRYGLKPSKIAFLAFHAWRDATAGAWPPQYPEERRRAYDLPTIRSWMEVFLYRFFTISQFKRSASPNGPKV 647          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: A0A7W6CPY5_9HYPH (Glutamine-dependent NAD(+) synthetase n=8 Tax=Rhizobium TaxID=379 RepID=A0A7W6CPY5_9HYPH)

HSP 1 Score: 868 bits (2242), Expect = 4.850e-310
Identity = 429/647 (66.31%), Postives = 511/647 (78.98%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHDA----WPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            M+FYS+Y QGFVRVAACTP CE+GD  +N   TL +AR G  +GV LMVFPELG+S YAIDDLLGQ ALL+ V+ +I E+ AAS  L+P++L+GAPL+ EGR YNC VAIH GRILGVVPK HLPNYREFYEKRWF SGR ++  TI +AG++VPFG+D+IFAAED A   FHIE+CED+W+P  PSDF ALA A IL NLSA NI +GKAETR+LLCA QS+R ++A++YSAAGPGESTTDLAWD QACIYELGS+L++T++FP  SQM +AD+DV+RL      TGTF +AA     P    RRI F F+    + GL + VARFP+ PAD +RLDQDC E ++IQ+QGLMKRLR+TGI+RLC+G+SG L ST A +VAA+A+D+ GWPRSD+L FTMP FAT + TK+NA ALM S+ VT E IDIKPLA Q+L  L+HPFS G+PVYDVTFENVQAGL T+ LFRAANQR A+VLGTGDLSEI  GW TYGVGDQMSHYNVNASVPKTLIQHLIRWV R+  FD     TL   L T+ISPELVP   +G +QS  +KIGPY+L DF  FY T +GL+PSK+A  A+ AW DA    WPP +P ++RRA+DLP IK WMEVFLFRFFTISQFKRSA+PNGPKV
Sbjct:    1 MNFYSVYDQGFVRVAACTPICEIGDPDFNSKATLDMARQGHARGVGLMVFPELGISGYAIDDLLGQDALLKAVDASIAEIVAASAALTPVLLIGAPLQHEGRLYNCAVAIHAGRILGVVPKVHLPNYREFYEKRWFASGRTVRDQTIRVAGETVPFGIDMIFAAEDRADFTFHIEICEDLWAPAAPSDFGALAGALILTNLSASNITVGKAETRKLLCAAQSSRSFSAYIYSAAGPGESTTDLAWDGQACIYELGSMLSETERFPMTSQMCVADIDVERLRLERLRTGTFNDAATLNLTPDRAFRRIGFQFQAPRGDIGLERDVARFPFVPADESRLDQDCYETFNIQVQGLMKRLRSTGIKRLCLGISGGLDSTHALLVAARAYDRLGWPRSDILCFTMPGFATGDETKSNAWALMRSIGVTAEEIDIKPLALQLLRDLKHPFSGGQPVYDVTFENVQAGLRTDFLFRAANQRNALVLGTGDLSEIGLGWSTYGVGDQMSHYNVNASVPKTLIQHLIRWVIRTGDFDADAKATLERILGTLISPELVPADENGVMQSTEDKIGPYDLHDFALFYTTRFGLRPSKVAFLAYCAWRDAEAGVWPPHFPRDKRRAFDLPVIKKWMEVFLFRFFTISQFKRSASPNGPKV 647          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: W6RDL2_9HYPH (Glutamine-dependent NAD(+) synthetase n=25 Tax=Rhizobium TaxID=379 RepID=W6RDL2_9HYPH)

HSP 1 Score: 868 bits (2242), Expect = 4.850e-310
Identity = 428/647 (66.15%), Postives = 509/647 (78.67%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHDA----WPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            M+FYSIY QGFVRVAACTP CE+GD  +N   TL LAR G  +G  LMVFPELG+S+Y+IDDLLGQ ALL+ V+ AI E+  AS DL+P++LVGAPL+  GR YNC VAIH GRILGVVPK HLPNYREFYEKRWF SGR ++  +I +AG++VPFG DLIFAAED+   +FHIE+CED+W+P  PSDF ALA A ILANLSA NI +GKA+TR+LLC+ QSAR  +A++YSAAGPGESTTDLAWD QACIYELGSLL +TD+FP  SQM +AD+DV+RL      TGTF +AA          RR+ F F+    + GL++ VARFP+ PAD +RLDQDC E ++IQ+QGLMKRLR+TGI+RLCIG+SG L ST A +VAA+AFDQ GWPRSD+L FTMP FAT + TK+NA ALM S+ VT E +DI+PLA Q+L  L+HPF  G+PVYD TFENVQAGL T+ LFRAAN R A+VLGTGDLSEI  GW TYGVGDQMSHYNVNASVPKTLIQHLIRWV RS  FD +   TL S L T+ISPELVP   +G +QS  +KIGPY+L DF  +Y T +GL+PSKIA  A++AW DA    WPP +P ++RR++DLP IK WMEVFLFRFFTISQFKRSA+PNGPKV
Sbjct:    1 MNFYSIYDQGFVRVAACTPNCEIGDPDFNAQATLELARQGHAEGAGLMVFPELGISSYSIDDLLGQDALLKGVDAAIAEIVRASRDLTPVLLVGAPLQNGGRLYNCAVAIHAGRILGVVPKMHLPNYREFYEKRWFASGRGVRGDSIRVAGETVPFGTDLIFAAEDMEDFVFHIEICEDLWAPAAPSDFGALAGALILANLSASNITVGKADTRKLLCSAQSARSLSAYVYSAAGPGESTTDLAWDGQACIYELGSLLAETDRFPTSSQMCVADIDVERLRLERLRTGTFNDAATLNLTAGHEFRRVAFQFKKPKGDIGLKRDVARFPFVPADESRLDQDCYETFNIQVQGLMKRLRSTGIKRLCIGISGGLDSTHALLVAARAFDQLGWPRSDILCFTMPGFATGDATKSNAWALMRSMGVTAEEVDIRPLALQLLKDLKHPFGSGKPVYDTTFENVQAGLRTDFLFRAANHRNALVLGTGDLSEIGLGWSTYGVGDQMSHYNVNASVPKTLIQHLIRWVIRSGDFDAEANATLESILGTLISPELVPADENGVMQSTEDKIGPYDLHDFALYYTTRFGLRPSKIAFLAYNAWRDAGAGVWPPHFPADKRRSFDLPVIKHWMEVFLFRFFTISQFKRSASPNGPKV 647          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: A0A8I1NSV0_9HYPH (Glutamine-dependent NAD(+) synthetase n=1 Tax=Hyphomicrobiales bacterium TaxID=1909294 RepID=A0A8I1NSV0_9HYPH)

HSP 1 Score: 862 bits (2226), Expect = 1.310e-307
Identity = 431/647 (66.62%), Postives = 499/647 (77.13%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHD----AWPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            M F S Y QGF+RVAACTP CE+ D   N+A TLALAR+G   GV LM FPELG+S Y+IDDLLGQ  LL  VE  I EL AAS  L P++LVGAPL + GR YNC +AIH GRILGVVPK HLPNYREFYEKRWF SGRD+++  I +AGQ+VPFG DLIF+A D     FHIE+CED+W+P PPSDF ALA A ILANLSA NI +GKA+TR+LLCA +SA  WAA++YSAAGPGESTTDLAWD QACIYELGSLL +T++FP  SQM +ADVDV+RL      TGTF +AA     P    RRI F F+    + GL + VARFP+ PAD ARLDQDC E ++IQ+ GLMKRL++TGI+RLCIG+SG L ST A +VAA+A+D+ GWPRSD+LGFTMP FAT + TKTNA ALM +L +T E +DI+PLA Q+L  L HPFS GEPVYDVTFENVQAGL T+ LFRAANQR A+VLGTGDLSEI  GW TYGVGDQMSHYNVNASVPKTLIQHLIRWVAR+         TL S LDT+ISPELVP    GA+QS  +KIGPY+L DF  FY T +GLKPSK+A  A+HAW D    AWPP +P E+RR + LP I+ WME FLFRFFTISQFKRSA+PNGPKV
Sbjct:    1 MDFASAYDQGFIRVAACTPACEIADPPANVAATLALAREGHAAGVGLMAFPELGISGYSIDDLLGQEVLLDAVEAGIAELLAASRSLRPVLLVGAPLAQNGRLYNCALAIHDGRILGVVPKTHLPNYREFYEKRWFASGRDVRNTQITLAGQTVPFGTDLIFSAADRPDFTFHIEICEDLWAPAPPSDFGALAGATILANLSASNITVGKADTRKLLCAAKSASNWAAYIYSAAGPGESTTDLAWDGQACIYELGSLLAETERFPAGSQMCMADVDVERLRLERLRTGTFNDAATLNLGPDKTFRRIGFDFDAPQGDIGLNRIVARFPFVPADAARLDQDCYETFNIQVHGLMKRLKSTGIKRLCIGISGGLDSTHALLVAARAYDRLGWPRSDILGFTMPGFATGDETKTNAWALMRALGITAEEVDIRPLALQLLKDLGHPFSGGEPVYDVTFENVQAGLRTDFLFRAANQRNALVLGTGDLSEIGLGWCTYGVGDQMSHYNVNASVPKTLIQHLIRWVARTGDLSPAANSTLESILDTIISPELVPADESGAIQSTEDKIGPYDLHDFFLFYTTRFGLKPSKVAFLAWHAWRDTDAGAWPPHFPAEKRREFPLPIIRKWMETFLFRFFTISQFKRSASPNGPKV 647          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: A0A7X0JJ79_9HYPH (Glutamine-dependent NAD(+) synthetase n=8 Tax=Rhizobium/Agrobacterium group TaxID=227290 RepID=A0A7X0JJ79_9HYPH)

HSP 1 Score: 858 bits (2218), Expect = 2.000e-306
Identity = 424/647 (65.53%), Postives = 501/647 (77.43%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHDA----WPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            M+F+S Y  GF+R AACTP CE+GD  +N   TL LAR+G  + VALMVFPELG+S Y+IDDLLGQ  LL  VE A+  +   S  L P++LVGAPL  EGR +NC V IH GRILGVVPK H+PNYREFYEKRWF SGR ++   I +AGQSVPFG+DL FAAE++   IFH+E+CEDVW P PPSDF A+A A IL NLSA NI +GKAE R+LLCATQSAR +AA++YSAAGPGESTTDLAWD QACIYELG LL +T++FP   QM +AD+DV+RL      TGTF +AA          RR++F FE       L +A+ R+P+ PADP RLDQDC E ++IQ+QGLMKRL++TGI+RLCIG+SG L ST A +VAAKA+DQ GWPR+D+LGFTMP FAT + TK+NA ALM  + +T E +DIKPLA Q+L  L+HPFS GEPVYDVTFENVQAGL T+ LFRAANQR A+VLGTGDLSEIA GW TYGVGDQMSHYNVNASVPKTLIQHLIRWV  + +FDD+T  TL   L+T+ISPELVP GADGA+QS  +KIGPY+L DFT +YLT +GLKPSKIA  A  AW DA    WP  +P E++R +DLP IK WME FLFRFFTISQFKRSA+PNGPKV
Sbjct:    1 MNFHSPYSHGFIRGAACTPVCELGDPDFNARATLDLAREGHARSVALMVFPELGISGYSIDDLLGQDVLLDAVESALATIVEESKALLPVLLVGAPLRHEGRLFNCAVVIHAGRILGVVPKTHIPNYREFYEKRWFASGRSVRDMMISVAGQSVPFGIDLQFAAENIRDFIFHVEICEDVWLPAPPSDFGAMAGALILTNLSASNITVGKAEMRKLLCATQSARNFAAYLYSAAGPGESTTDLAWDGQACIYELGDLLGETERFPAGPQMCVADIDVERLRLERMRTGTFNDAATLHLTAGKTFRRVLFDFERPQETLDLERAIQRYPFVPADPKRLDQDCYETFNIQVQGLMKRLKSTGIKRLCIGISGGLDSTHALLVAAKAYDQLGWPRTDILGFTMPGFATGDDTKSNAWALMRGMGITAEEVDIKPLATQLLHDLKHPFSGGEPVYDVTFENVQAGLRTDFLFRAANQRNALVLGTGDLSEIALGWSTYGVGDQMSHYNVNASVPKTLIQHLIRWVIEAESFDDETNATLQRILNTLISPELVPAGADGAMQSTEDKIGPYDLHDFTLYYLTRFGLKPSKIAYMALQAWKDAGAGAWPSHFPEEKKRQFDLPVIKRWMETFLFRFFTISQFKRSASPNGPKV 647          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: UPI00177D741E (NAD(+) synthase n=1 Tax=Rhizobium sp. CFBP 8762 TaxID=2775279 RepID=UPI00177D741E)

HSP 1 Score: 856 bits (2211), Expect = 2.230e-305
Identity = 421/647 (65.07%), Postives = 498/647 (76.97%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHDA----WPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            M+F+ IY QGFVRVAACT +CEV D  +N   TL L R G  Q VALMVFPELGLS YAIDDLLGQ ALL  VE A+ +L AAS DL P+ LVGAPL  +GR YNC V IH GR+LGVVPK HLPNYREFYEKRWF SGR++++A I+IAGQ+ PFG+DL+FA    A  +FH+E+CED+W+P PPSD+ ALA A IL NLSA NI +GKA+ R+LLCA+QSAR W+A++YSAAGPGESTTDLAWD QACIYE+G+LL +TD+FP  SQM ++D+D+DRL      TGTF +AA          RR+ F F     + G  +A+ARFP+ PADP RLDQDC E ++IQ+QGLMKRL +TGI RLCIGVSG L ST A +VAA+AFD+ GWPR+D+LGFTMP FAT + TK NA +LM +L +T E IDIKPLA Q+L  L+HPF+ GEPVYDVTFENVQAGL T+ LFRAANQRGA+VLGTGDLSEIA GW TYGVGD MSHYNVNASVPKTLIQHLIRW+     F D T  TLL+ L T ISPELVP    G +QS  +KIGPY+L DF  +Y+T +GLKPS++A  A+HAWHD     WP   P + RR+YDLP IK W+E FLFRFFTISQFKRSA+PNGPKV
Sbjct:    1 MNFHGIYDQGFVRVAACTSECEVADPTFNATATLDLWRRGHAQHVALMVFPELGLSGYAIDDLLGQDALLDAVEDAVRDLVAASADLLPVALVGAPLRHQGRIYNCAVVIHAGRVLGVVPKVHLPNYREFYEKRWFASGRNIRNAMIDIAGQTAPFGVDLLFADAQDADFVFHVEVCEDLWAPAPPSDYGALAGAYILTNLSASNITVGKADMRKLLCASQSARTWSAYIYSAAGPGESTTDLAWDGQACIYEMGTLLAETDRFPSGSQMCVSDIDLDRLRLERMRTGTFNDAATLTLGENHTFRRLTFDFRKPQDDVGFERAIARFPFVPADPLRLDQDCYETFNIQVQGLMKRLASTGISRLCIGVSGGLDSTHALLVAARAFDKLGWPRTDILGFTMPGFATGDATKGNAWSLMRNLGITAEEIDIKPLALQLLQDLKHPFAGGEPVYDVTFENVQAGLRTDFLFRAANQRGALVLGTGDLSEIALGWSTYGVGDHMSHYNVNASVPKTLIQHLIRWLVSKGEFHDDTNETLLAILGTTISPELVPANEQGEIQSTEDKIGPYDLHDFFLYYITRFGLKPSRVAFMAYHAWHDLEAGHWPAGMPEDNRRSYDLPVIKRWLETFLFRFFTISQFKRSASPNGPKV 647          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: A0A558RDD6_9SPHN (Glutamine-dependent NAD(+) synthetase n=3 Tax=Sphingomonas TaxID=13687 RepID=A0A558RDD6_9SPHN)

HSP 1 Score: 854 bits (2207), Expect = 3.550e-304
Identity = 426/645 (66.05%), Postives = 498/645 (77.21%), Query Frame = 1

Query:    7 FYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHD----AWPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            F SIY+QGFVRVAAC P C + D  +NL  TLALAR+G+  GVALMVFPELGLS Y+IDDL  Q ALL  VE+ + +L +ASCDL P++LVGAPL REGR YNC VA+HRGRILGVVPKGHLPNYREFYEKRWF SGRD++   I + G  VPFGMDL+F A+D+ G IFHIE+CEDVW+P PPSDFAALA A IL NLSA NI++GKA+TRR LC  QS RCWAA++YSAAG GESTTDLAWD QACIYELG+ + +T +F  DSQM +AD+DV+RL      TGTF EAAIA  MP  R RR+ F  +P + N GLR+ + RFP+ P DPARLDQDC EA++IQ+ GL +RL ATGI ++CIGVSG L ST A IVAAKAFD  G PR+D+LGFTMP FAT   TK NA ALM  L VT E IDIKPLA QMLA LQHPF+ G+PVYD TFENVQAGL T++LFRAANQR A+VLGTG++SE+A GW TYGVGDQMSHYNVN S+PKTLIQHLIRW A +  FD   T  LL  L T ISPEL+P  ++G  QS   KIGPYEL DF  +Y T +GL PSKIA  A HAW D    +WPP +P E++RAY+L +I++W+EVFL RFF  +QFKRSA PNGPKV
Sbjct:   17 FLSIYQQGFVRVAACRPTCRIADPAFNLEATLALAREGSDAGVALMVFPELGLSGYSIDDLFLQRALLDEVERGLLDLVSASCDLHPVLLVGAPLRREGRLYNCAVAVHRGRILGVVPKGHLPNYREFYEKRWFASGRDVRGE-ISVGGDPVPFGMDLLFQADDVDGLIFHIEICEDVWAPAPPSDFAALAGALILTNLSASNIVVGKADTRRRLCELQSGRCWAAYIYSAAGYGESTTDLAWDGQACIYELGAQIAETPRFSVDSQMVVADIDVERLQLERMRTGTFNEAAIANGMPDRRFRRVSFHLDPPLGNLGLRRHIDRFPFVPDDPARLDQDCYEAFNIQVSGLARRLEATGIAKICIGVSGGLDSTHALIVAAKAFDLLGRPRTDILGFTMPGFATSARTKLNAHALMSGLGVTAEEIDIKPLANQMLADLQHPFAMGQPVYDTTFENVQAGLRTDLLFRAANQRNALVLGTGNMSELALGWCTYGVGDQMSHYNVNGSLPKTLIQHLIRWTASNRLFDTNVTDVLLDVLGTTISPELIPQASEGESQSTEGKIGPYELHDFWIYYFTRFGLPPSKIAFLALHAWQDVGAGSWPPGFPEEQKRAYELGAIRAWLEVFLRRFFA-NQFKRSALPNGPKV 659          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: A0A0Q6SJ95_9HYPH (Glutamine-dependent NAD(+) synthetase n=1 Tax=Rhizobium sp. Root1212 TaxID=1736429 RepID=A0A0Q6SJ95_9HYPH)

HSP 1 Score: 852 bits (2202), Expect = 5.000e-304
Identity = 422/647 (65.22%), Postives = 501/647 (77.43%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADRLRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHDA----WPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            M FYS Y  GFVR A CTP+CEV D  +N A  L LAR+G  Q VALMVFPEL +S YA+DDLLGQ  LL  VE AI+ + +AS DLSP++LVGAPL  E R +NC V IH GR+LGVVPK HLPNYREFYEKRWF SGR ++ A I +AGQ VPFG DL+FAA+++A  IFH+E+CED+W+P PPSDF ALA A ILANLSA NI +GKA+ R+LLC+ QSAR W+A++YSAAGPGESTTDLAWD QACIYELG LL +T++FP  SQM IAD+D++RL      TGTF +AA   +  A   RRI F F+       L + +AR+P+ PADP RLDQDC E ++IQ+QGLMKRL+ TGI+RLCIGVSG L ST A +VAA+ FD+  WPRSD+LGFTMP FAT + TKTNA ALM SL +T E IDIKPLA Q+L  L+HPF+ GEPVYDVTFENVQAGL T+ LFRAANQRGA+VLGTGDLSEIA GW TYGVGDQMSHYNVNASV KTLIQHL+RWV +S  FD +T  TL S LDTVISPELVP    G +QS  ++IGPY+L DF  +Y+T +GLKPSK+A  A++AW D     WP  +P  ++R +DLP+IKSW+EVFLFRFFT SQFKRSA+PNGPKV
Sbjct:    1 MEFYSPYSHGFVRCATCTPECEVADPEFNAAAILKLAREGHDQRVALMVFPELSISGYAVDDLLGQDVLLDAVETAIDTIVSASKDLSPVLLVGAPLRHESRLFNCAVVIHAGRVLGVVPKTHLPNYREFYEKRWFASGRTVQDAEISVAGQVVPFGRDLLFAAKNVADFIFHVEICEDLWAPAPPSDFGALAGALILANLSASNITVGKADVRKLLCSAQSARTWSAYLYSAAGPGESTTDLAWDGQACIYELGDLLAETERFPDGSQMCIADIDLERLRLERMRTGTFNDAATL-NADAKAFRRIAFEFQMPSGRIELFRPIARYPFVPADPGRLDQDCYETFNIQVQGLMKRLKTTGIKRLCIGVSGGLDSTHALLVAARVFDRLDWPRSDILGFTMPGFATGDATKTNAWALMRSLGITAEEIDIKPLATQLLTDLKHPFADGEPVYDVTFENVQAGLRTDFLFRAANQRGALVLGTGDLSEIALGWSTYGVGDQMSHYNVNASVSKTLIQHLVRWVIKSELFDQETGKTLGSILDTVISPELVPADDQGLIQSTEDRIGPYDLHDFFLYYITRFGLKPSKVAYLAYNAWRDRETGEWPAHFPEAKKRDFDLPTIKSWLEVFLFRFFTTSQFKRSASPNGPKV 646          

BLAST of mRNA_P-wetherbeei_contig682.2.1 vs. uniprot
Match: A0A0Q8NKI7_9HYPH (Glutamine-dependent NAD(+) synthetase n=2 Tax=Rhizobium TaxID=379 RepID=A0A0Q8NKI7_9HYPH)

HSP 1 Score: 852 bits (2200), Expect = 1.160e-303
Identity = 417/648 (64.35%), Postives = 512/648 (79.01%), Query Frame = 1

Query:    1 MSFYSIYRQGFVRVAACTPKCEVGDSGYNLAETLALARDGARQGVALMVFPELGLSAYAIDDLLGQAALLQRVEQAIEELAAASCDLSPIVLVGAPLEREGRRYNCVVAIHRGRILGVVPKGHLPNYREFYEKRWFTSGRDLKSATIEIAGQSVPFGMDLIFAAEDLAGSIFHIELCEDVWSPVPPSDFAALASAPILANLSAINIIIGKAETRRLLCATQSARCWAAHMYSAAGPGESTTDLAWDDQACIYELGSLLTKTDQFPQDSQMAIADVDVDRLS-----TGTFKEAAIAQDMPADR-LRRIVFGFEPAMANHGLRKAVARFPYAPADPARLDQDCNEAYSIQMQGLMKRLRATGIRRLCIGVSGSLCSTQAFIVAAKAFDQFGWPRSDMLGFTMPSFATVEVTKTNACALMHSLRVTGEAIDIKPLAQQMLATLQHPFSRGEPVYDVTFENVQAGLHTNILFRAANQRGAMVLGTGDLSEIAPGWPTYGVGDQMSHYNVNASVPKTLIQHLIRWVARSNAFDDKTTHTLLSTLDTVISPELVPPGADGALQSAGEKIGPYELQDFTPFYLTCYGLKPSKIAVPAFHAWHDA----WPPQYPNERRRAYDLPSIKSWMEVFLFRFFTISQFKRSATPNGPKV 1914
            M+FYS+Y QGFVRVAACTP CE+GD  +N A TL LAR G  + V LMVFPELG+S Y+IDDLLGQ ALL+ V+ AI ++  AS DL+P+++VGAPL+  GR YNC +AIH G++LGVVPK HLPNYREFYEKRWF SGRD++  +I +AG  VPFG DLIFAAE+L   IFH+E+CED+W+P PPSDF ALA A +LANLSA NI +GKA+TR+LLC+ QSAR  +A++YSAAGPGESTTDLAWD QAC+YELG++L +TD+FP +SQM +ADVD++RL      TGTF +AA   ++ ADR  R + F F     + G  + VARFP+ PAD +RLDQDC E ++IQ+QGLMKRL +TGI+RLC+G+SG L ST A +VAA+AFD+ GWPRSD+L FTMP FAT + TK+NA +LM S+ V+ E +DIKPLA Q+L  L+HPF+ GEPVYD TFENVQAGL T+ LFRAANQR A VLGTGDLSEI  GW TYGVGDQMSHYNVNASVPKTLIQHLIRWV R++ FD +T  TL   L T+ISPELVP   +G +QS  +KIGPY+L DF  +Y T +GL+PSK+A  A+ AW DA    WPP +P ++RR++DLP+I+ WMEVFLFRFFTISQFKRSA+PNGPKV
Sbjct:    1 MNFYSVYDQGFVRVAACTPSCEIGDPDFNAAATLELARQGHTRSVGLMVFPELGISGYSIDDLLGQDALLRSVDAAIADIVRASKDLTPVLIVGAPLQSGGRLYNCAIAIHAGKVLGVVPKTHLPNYREFYEKRWFASGRDVRGRSITVAGDVVPFGTDLIFAAEELQDFIFHVEICEDLWAPAPPSDFGALAGALVLANLSASNITVGKADTRKLLCSAQSARSLSAYIYSAAGPGESTTDLAWDGQACVYELGTMLAETDRFPTESQMCVADVDLERLRLERLRTGTFNDAATL-NLTADRQFRTVRFSFAKPAGDVGFERDVARFPFVPADASRLDQDCYETFNIQVQGLMKRLSSTGIKRLCLGISGGLDSTHALLVAARAFDRLGWPRSDILCFTMPGFATGDETKSNAWSLMRSMGVSAEEVDIKPLALQLLRDLKHPFAGGEPVYDTTFENVQAGLRTDFLFRAANQRNAFVLGTGDLSEIGLGWSTYGVGDQMSHYNVNASVPKTLIQHLIRWVIRTSDFDAETKATLERILGTLISPELVPADENGVMQSTEDKIGPYDLHDFFLYYTTRFGLRPSKVAFLAYQAWSDAKSGVWPPHFPADKRRSFDLPTIRRWMEVFLFRFFTISQFKRSASPNGPKV 647