mRNA_P-wetherbeei_contig12751.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig12751.2.1
Unique NamemRNA_P-wetherbeei_contig12751.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: A0A7R9YDS6_9STRA (Glycine--tRNA ligase n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YDS6_9STRA)

HSP 1 Score: 194 bits (492), Expect = 1.140e-56
Identity = 105/156 (67.31%), Postives = 120/156 (76.92%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESWR 423
            GYVS++E   + AEAE  A+++ RK+ + GALKLP++L+DLTE  PEEI LVPSPATGKPGSLT PRAFNLMFQT VGAM+DASS AYLRPETAQGIF NFK                IGK FRNEITPRNFIFRSREFEQMEVEYFI  +++ WR
Sbjct:  148 GYVSVMEDAAMQAEAEKKAEKM-RKSAQAGALKLPIELKDLTEATPEEIELVPSPATGKPGSLTPPRAFNLMFQTQVGAMSDASSTAYLRPETAQGIFVNFKTTQNVARGKLPFGIAQIGKAFRNEITPRNFIFRSREFEQMEVEYFIPPEDDIWR 302
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: A0A835YRJ7_9STRA (Glycine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YRJ7_9STRA)

HSP 1 Score: 192 bits (489), Expect = 1.670e-56
Identity = 102/156 (65.38%), Postives = 120/156 (76.92%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESWR 423
            G VS++E   + AEA+AAAD++KRKA KQGAL  PL L+DLT+   E++ ++PSPATG PG+LTAPR FNLMFQT+VGA  D S+ AYLRPETAQGIFTNFKN               IGK FRNEITPRNFIFRSREFEQMEVEYFI DD+++WR
Sbjct:  113 GAVSVMESGTMEAEAQAAADKMKRKAAKQGAL-APLDLKDLTQATAEDLSIIPSPATGNPGTLTAPRDFNLMFQTAVGATVDGSNAAYLRPETAQGIFTNFKNVQQTARAKIPFGIAQIGKAFRNEITPRNFIFRSREFEQMEVEYFISDDDDAWR 267
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: W7TM99_9STRA (Glycine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TM99_9STRA)

HSP 1 Score: 192 bits (487), Expect = 1.260e-55
Identity = 98/155 (63.23%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESW 420
            GY++LL+  D+  EA   A++LK+KAGK+G +K PL ++D+T+    E+ L+PSPATG+PGSLT PRAFNLMFQTSVGA+ DASS A+LRPETAQGIFTNFKN               IGK FRNEITPRNFIFRSREFEQME+EYFI  +EE W
Sbjct:  199 GYIALLDSGDLHGEARKLAEKLKKKAGKEGTIKFPLDIKDMTKATVAEMALIPSPATGEPGSLTPPRAFNLMFQTSVGALADASSTAFLRPETAQGIFTNFKNVQSTGRVKVPFGIAQIGKAFRNEITPRNFIFRSREFEQMEIEYFIPPEEEVW 353
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: D8LCT3_ECTSI (Glycine--tRNA ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCT3_ECTSI)

HSP 1 Score: 187 bits (474), Expect = 3.520e-55
Identity = 99/150 (66.00%), Postives = 121/150 (80.67%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKNI--------GKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESWRQ 426
            GYVS+LE   +  EA+ AA+++KRKA KQG LK  + L+DL+ ++ EE+ LVPSPATG PG+LT PR FNLMFQT+VGAM DASSVAY+RPETAQGIFTNFKN+        GK FRNEITPRNFIFRSREFEQMEVEYF+++ + +W+Q
Sbjct:  226 GYVSVLESGTMQEEADTAANKIKRKAAKQGTLKA-VSLQDLSRISAEELELVPSPATGTPGALTPPREFNLMFQTNVGAMADASSVAYMRPETAQGIFTNFKNVQQSARAKVGKAFRNEITPRNFIFRSREFEQMEVEYFVEEGD-AWKQ 373
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: A0A257QHU4_9BACT (Glycine--tRNA ligase n=1 Tax=Verrucomicrobia bacterium 21-51-4 TaxID=1970609 RepID=A0A257QHU4_9BACT)

HSP 1 Score: 186 bits (471), Expect = 7.730e-54
Identity = 100/155 (64.52%), Postives = 116/155 (74.84%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESW 420
            GY+SLLE  D+  +A+ AA+ +KRKA KQG L+  ++LR  TE  PEE   +PSPATGKPGSLT PR+FNLMF T VGA+ DASSVAYLRPETAQGIF NFKN               IGK FRNEITPRNFIFRSREFEQME+EYFI+ D+E+W
Sbjct:  127 GYISLLESGDMEEQAQKAAELMKRKAAKQGTLEA-VKLRPYTEAKPEEYSQIPSPATGKPGSLTPPRSFNLMFSTHVGALEDASSVAYLRPETAQGIFANFKNVTDSTRVKIPFGIAQIGKAFRNEITPRNFIFRSREFEQMEIEYFINPDDETW 280
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: A0A7S0P4G7_9EUKA (Glycine--tRNA ligase (Fragment) n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0P4G7_9EUKA)

HSP 1 Score: 184 bits (468), Expect = 1.700e-53
Identity = 102/157 (64.97%), Postives = 117/157 (74.52%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESWRQ 426
            GYVSLLE  D+  +A A A ++KRKA  QG+L L  +L+D TE  P+E  LVPSP TGKPG+LTAPR FNLMFQT+VGAM DA+SVAYLRPETAQGIFTNF+N               IGK FRNEITPRNFIFRSREFEQME+E+FI  DE+ W Q
Sbjct:  137 GYVSLLESGDMEEKAMAMAQKMKRKAKLQGSLTLS-ELKDFTEATPDEQALVPSPETGKPGALTAPRDFNLMFQTNVGAMADAASVAYLRPETAQGIFTNFRNVVSTGRVKVPFGIAQIGKAFRNEITPRNFIFRSREFEQMEIEFFIPPDEDVWAQ 292
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: A0A6U1N9R4_9STRA (Glycine--tRNA ligase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A6U1N9R4_9STRA)

HSP 1 Score: 184 bits (466), Expect = 3.220e-53
Identity = 97/157 (61.78%), Postives = 118/157 (75.16%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESWRQ 426
            GY+SLLE  D+  +AE  A ++K+KA KQG LK P++L+++ E   EE+ L+PSPATG PG+LTAPR FNLMFQT VGA+ DAS+VAYLRPETAQGIFTNFKN               IGK FRNEITPRNFIFRSREFEQME+EYFI   ++ W++
Sbjct:  113 GYLSLLESGDMQDQAEKQAKKMKKKAEKQGTLK-PIELKEVAEATEEELALIPSPATGTPGALTAPREFNLMFQTQVGALADASAVAYLRPETAQGIFTNFKNVVNTARIKLPFGIAQIGKAFRNEITPRNFIFRSREFEQMEIEYFIPPGDDQWQE 268
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: A0A1J4XFE5_9BACT (Glycine--tRNA ligase n=1 Tax=Verrucomicrobia bacterium CG1_02_43_26 TaxID=1805421 RepID=A0A1J4XFE5_9BACT)

HSP 1 Score: 182 bits (463), Expect = 1.040e-52
Identity = 100/155 (64.52%), Postives = 114/155 (73.55%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESW 420
            GYVS+LE   +  EA+ AA+ LKRK  KQG+LK  + L+  TE  PEE  LVPSPATGKPGSLTAPR FNLMF+TSVGAM D SS+AYLRPETAQGIF NFKN               IGK FRNEITPRN+IFRSREFEQME+EYFI   +++W
Sbjct:  121 GYVSVLESGTMQEEADKAAEALKRKLAKQGSLKA-VALKPFTEAKPEEYELVPSPATGKPGSLTAPRDFNLMFETSVGAMRDESSIAYLRPETAQGIFANFKNVVDTTRVKVPFGIAQIGKAFRNEITPRNYIFRSREFEQMEIEYFIPPQDDAW 274
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: A0A257LRY4_9BACT (Glycine--tRNA ligase (Fragment) n=1 Tax=Verrucomicrobiales bacterium VVV1 TaxID=2015575 RepID=A0A257LRY4_9BACT)

HSP 1 Score: 174 bits (441), Expect = 1.560e-52
Identity = 95/151 (62.91%), Postives = 109/151 (72.19%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDD 408
            GYVS LE  +   E + AA+  KRK   QG L  P+  +DLTE   +EI L+PSPATG+PGSLT PRAFN+MFQT+VGAMTD SSVAYLRPETAQG+F +FKN               IGK FRNEITPRNFIFRSREFEQME+EYFI +D
Sbjct:    7 GYVSALESENTAGELQVAAESFKRKKAIQGTLS-PVVAKDLTEAREDEIALIPSPATGEPGSLTPPRAFNMMFQTNVGAMTDGSSVAYLRPETAQGMFVDFKNVVDTGRVKLPFGIAQIGKSFRNEITPRNFIFRSREFEQMEMEYFIHED 156
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BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Match: A0A6U2HYH8_HEMAN (Glycine--tRNA ligase n=2 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A6U2HYH8_HEMAN)

HSP 1 Score: 183 bits (464), Expect = 3.390e-52
Identity = 100/155 (64.52%), Postives = 115/155 (74.19%), Query Frame = 1 
Query:    1 GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIPLVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTNFKN---------------IGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESW 420
            GYVS++E   +  EA+  A+ +KRKA KQG LK P+ L ++ E   +E+  VPSPATGKPGSLT PRAFNLMFQT VGAM+DASS AYLRPETAQGIF NFKN               IGK FRNEITPRNFIFRSREFEQME+EYFIDD +E+W
Sbjct:  210 GYVSVMESGTMQEEADKTANTMKRKAQKQGTLK-PVTLAEVAECPLDELENVPSPATGKPGSLTPPRAFNLMFQTQVGAMSDASSTAYLRPETAQGIFVNFKNVQQTARTTLPFGIAQIGKAFRNEITPRNFIFRSREFEQMEIEYFIDDADETW 363
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig12751.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7R9YDS6_9STRA1.140e-5667.31Glycine--tRNA ligase n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A835YRJ7_9STRA1.670e-5665.38Glycine--tRNA ligase n=1 Tax=Tribonema minus TaxID... [more]
W7TM99_9STRA1.260e-5563.23Glycine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID... [more]
D8LCT3_ECTSI3.520e-5566.00Glycine--tRNA ligase n=1 Tax=Ectocarpus siliculosu... [more]
A0A257QHU4_9BACT7.730e-5464.52Glycine--tRNA ligase n=1 Tax=Verrucomicrobia bacte... [more]
A0A7S0P4G7_9EUKA1.700e-5364.97Glycine--tRNA ligase (Fragment) n=1 Tax=Calcidiscu... [more]
A0A6U1N9R4_9STRA3.220e-5361.78Glycine--tRNA ligase n=1 Tax=Fibrocapsa japonica T... [more]
A0A1J4XFE5_9BACT1.040e-5264.52Glycine--tRNA ligase n=1 Tax=Verrucomicrobia bacte... [more]
A0A257LRY4_9BACT1.560e-5262.91Glycine--tRNA ligase (Fragment) n=1 Tax=Verrucomic... [more]
A0A6U2HYH8_HEMAN3.390e-5264.52Glycine--tRNA ligase n=2 Tax=Hemiselmis andersenii... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig12751contigP-wetherbeei_contig12751:443..1663 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D8LCT3
PFAMsHGTP_anticodon,tRNA-synt_2b
Model size426
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons3
Evalue5.77e-56
EggNOG OGsCOG0423@1|root,KOG2298@2759|Eukaryota
Ec32 ortholog descriptionGlycyl-tRNA Synthetase (N-terminal region)
Ec32 orthologEc-07_006790.1
Descriptionglycyl-tRNA aminoacylation
Cds size426
COG categoryJ
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185959.8560953-CDS-P-wetherbeei_contig12751:442..5591683185959.8560953-CDS-P-wetherbeei_contig12751:442..559Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig12751 443..559 -
1683185959.8669426-CDS-P-wetherbeei_contig12751:803..8691683185959.8669426-CDS-P-wetherbeei_contig12751:803..869Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig12751 804..869 -
1683185959.876774-CDS-P-wetherbeei_contig12751:1420..16631683185959.876774-CDS-P-wetherbeei_contig12751:1420..1663Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig12751 1421..1663 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig12751.2.1prot_P-wetherbeei_contig12751.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig12751 443..1663 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig12751.2.1

>prot_P-wetherbeei_contig12751.2.1 ID=prot_P-wetherbeei_contig12751.2.1|Name=mRNA_P-wetherbeei_contig12751.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=142bp
GYVSLLEGPDIVAEAEAAADRLKRKAGKQGALKLPLQLRDLTELAPEEIP
LVPSPATGKPGSLTAPRAFNLMFQTSVGAMTDASSVAYLRPETAQGIFTN
FKNIGKDFRNEITPRNFIFRSREFEQMEVEYFIDDDEESWRQ
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mRNA from alignment at P-wetherbeei_contig12751:443..1663-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig12751.2.1 ID=mRNA_P-wetherbeei_contig12751.2.1|Name=mRNA_P-wetherbeei_contig12751.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1221bp|location=Sequence derived from alignment at P-wetherbeei_contig12751:443..1663- (Phaeothamnion wetherbeei SAG_119_79)
GGCTACGTGTCCCTGCTGGAAGGGCCGGACATAGTCGCCGAGGCGGAGGC
CGCCGCTGACCGCCTGAAGCGCAAGGCCGGCAAGCAGGGCGCCCTCAAGC
TGCCGCTTCAGCTGCGGGACCTCACAGAGCTAGCTCCCGAGGAGATCCCG
CTGGTACCGTCGCCTGCTACAGGTAAACCGGGTTCGCTGACGGCGCCGCG
GGCGTTCAACCTGATGTTCCAGACCTCGGTCGGGGCCATGACGGTGAGTG
GGCGAGCGGTGGTGGCGGTGAATGTGCCCAGGCTGGCGGGGCCGCGAACC
CGGTTAGATGGGGTCTTCCTGGCCATGCTTCTTGTTTTAATTTGGCGTAC
ATTCCTTCTTCCGCCGCGCGGGCTCCTTTAGTAGTTTCAGAAGTTCTCTT
TTTGCACGCTGTCTCATTGAGCTGTTTTTCCTTTCCTCTCACCGATTGTC
TTCTCTTCCATATGTGCGTGCTGGGACCGGCTGAGGGCAATTATTCCTTT
TTTATGGTCATGAATCGTTTAATTTCCATCATGCTGGAAACTTTGTAAAG
TCTCTTCCTCAAGCAGGAATTTGGCGACATTCTTGCACTCCGCCTTTATT
CTTGCATGTCCACCCAGCTCTTCGCCGCTGCTTCCGCCGTGGATGCAGCT
GAATGCCTGTTCTCTTCTGCCGTTGTGGCCACCGATGCCGGCTGCCCCAC
TGCCGTCGTCACCGCTTCGATGCTCTCGTGTTTTTGCCTTTCTGCTTCGC
TGCTGGCGGGCCATTCTCGGGACGCTTGCTTCCGCCGAGCGCAGGACGCG
TCGTCGGTGGCGTACCTGCGGCCGGAGACGGCGCAGGGCATCTTCACCAA
TTTCAAGAACGTGCAGCAGTCAGCCCGCGCCAAGCTGCCCTTCGGAATCG
CACAGGCAAATAATGCTCGAATGTTGAATGGCTGACCTGTGCCAGGCGGA
AGCTGACGCTCCACACCAAAGAATTTCAATTGATATGAATGATTTGCGTT
CTTCTTGTTGTCCCATTTTTGCCCGAGTTTGTGTGCTCGGATGCTGTACA
CACACAATCGCCTGCCTCTTGCACACTTTTCTTGATTTCTTCCCTCGCGG
GCAGATCGGAAAAGACTTCCGGAACGAGATCACACCGCGAAATTTCATCT
TCCGCTCGCGGGAGTTTGAGCAGATGGAGGTTGAATACTTCATCGACGAT
GACGAGGAGTCGTGGCGGCAG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig12751:443..1663-

>mRNA_P-wetherbeei_contig12751.2.1 ID=mRNA_P-wetherbeei_contig12751.2.1|Name=mRNA_P-wetherbeei_contig12751.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=426bp|location=Sequence derived from alignment at P-wetherbeei_contig12751:443..1663- (Phaeothamnion wetherbeei SAG_119_79)
GGCTACGTGTCCCTGCTGGAAGGGCCGGACATAGTCGCCGAGGCGGAGGC
CGCCGCTGACCGCCTGAAGCGCAAGGCCGGCAAGCAGGGCGCCCTCAAGC
TGCCGCTTCAGCTGCGGGACCTCACAGAGCTAGCTCCCGAGGAGATCCCG
CTGGTACCGTCGCCTGCTACAGGTAAACCGGGTTCGCTGACGGCGCCGCG
GGCGTTCAACCTGATGTTCCAGACCTCGGTCGGGGCCATGACGGACGCGT
CGTCGGTGGCGTACCTGCGGCCGGAGACGGCGCAGGGCATCTTCACCAAT
TTCAAGAACATCGGAAAAGACTTCCGGAACGAGATCACACCGCGAAATTT
CATCTTCCGCTCGCGGGAGTTTGAGCAGATGGAGGTTGAATACTTCATCG
ACGATGACGAGGAGTCGTGGCGGCAG
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