mRNA_P-wetherbeei_contig656.5.2 (mRNA) Phaeothamnion wetherbeei SAG_119_79

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: A0A6H5JBC1_9PHAE (EF-hand domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBC1_9PHAE)

HSP 1 Score: 452 bits (1163), Expect = 2.970e-140
Identity = 310/886 (34.99%), Postives = 443/886 (50.00%), Query Frame = 3

Query:   18 EEDLMIGEIKTVKLGREARALGRYICYSLWLIVPCLTVHLLHK--LGGIDNHYSVATVSVLYWSIFFSAALLSLPFLRLTARTFAYCAMRYGSKLLYRSNTLYFAVAMHYDVALLAWSVFLLVAWRRVLLQYIDQSGGNGSNVADAV-DNVLTSYVAFRVGWLVKDFAVLIIATKYLWRPYLERVQTNIFAQYVLLMLTDYVTKGYIDRDDERFALQISNKGMR-WDRLSLYAVSKAIGFIPRHKLGHEFFKDLLESNDTQLDSAKEARVLGNFLFEQLIFIAAGKS-------QRGGSRFNRYGRYGSVTGGNGSGA----------GATLTPEDPLSRMAAGNFGATGNTMFSVVGTEDNSATLRPPTAASVFAGHYLTIG---RNALGRSPLPTTSSR-------------------DSXXXXXXXTVXXX----SXXASXXXXXXXXXSGSG-----------------IVPPID--------------------------PKERERD--------ELAEVLTRVKH------------------------------------------------------------LDQETFCPHLDPLLAEKAFAMFDIDGNGEATREEMVQGVVHTFHDHKSLSLTLKDSQHIAVKLGQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVMIHDDRGKL----QNCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALVGIEDSTKLTVRICFRQKSNFQDLSRYWAQHSICTQAIKEACDDLAITYSLPELPIGLKQKL 2189
            E DLMIGEI+++++  E   L R    +L LI+  L V  L +   G +D   ++  V     +IF     LS P +R T R FAY      S LLYR N+ Y+ VA+H D+++  WSV  +  WR++   ++  +    S       D +   YVA+RVG L+K+F VL++AT YLWRPYL+RVQ++I AQY+LL+LTDY   G  + DD++FA+++  +G++  +++SLYAVSKA+GFIPRHKLGH FF++L  SN   L+S+++A+ LG ++F+QL+  +   S            R+      G    G GS A          G  L P +  S  ++      G+ +    GT  ++    PP   S         G   R A G SP+ T  S                    DS       TV       +  +           G G                 ++  +D                          P  R  D        ++AE+   + +                                                            L +ET CP L+  + E AF +FD+D NG  T+ EMV GVV TF DH+SL+ TL DS+HIA KLG II  +I         ++ GFDV +LS+TFASFL+A +FMIG AASNL  +++++FV R+YDVGDRV I+DD         N VV K++L  T F+RWD+Q FYIPN +L+ +T +N+QR+ HQWHEF+I VAA T+  +L  L   LQ+FA+  D P+ LY  M F+L GIEDST+L++RI FRQ+ N+Q + + WA  S+CT AIK ACD + ITY LPE+P+ +K K+
Sbjct:  292 ETDLMIGEIQSIQIADEI-VLARK---ALMLILGSLVVATLFREFAGRLDKDVNIVNVVGFASTIF-----LSYPMIRFTIRLFAYFLTTRASALLYRGNSFYYVVALHQDISMTIWSVASMFVWRQLFAGWVYPTDSELSGKTYRYGDELFQCYVAYRVGMLLKNFLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYAATGKCNPDDQKFAIEMGRRGIKEQEKISLYAVSKAMGFIPRHKLGHAFFRELGGSNT--LNSSRDAKTLGEYMFDQLLLRSRTYSPGCTPPASPSTKRWAYLANVGQTVLGGGSNANTLMSARSFRGGRLGPSNRASLPSSWTASDVGS-LSPAKGTSSDATLKPPPRTRSRSGAEERAAGSAPREAGGGSPIVTRGSSKLSSSVDKMRMGPNARAASDSAAGAAAVTVDTADASDAGSSERPRNEVLPSEGGGGMARFREFHGGGKRALSVIQDVDSFADSELFVAGESKAEGTASGGEMEFPSLRVVDGMAPRHGHDVAELAKEIANGGSFSSQDRNDNDAGDASRGKDSADGTVQKEHEESIVQGNGSTQKGGSSDEVDKPQEEELLTRETLCPMLEEQVLEMAFTIFDMDNNGSITKAEMVLGVVDTFKDHRSLAHTLHDSEHIARKLGLIIFWLILFILAFVWLSILGFDVLSLSVTFASFLIAFSFMIGTAASNLMTAVLFIFVSRLYDVGDRVHIYDDVQTAGVEPMNVVVTKVDLRTTSFRRWDEQIFYIPNHLLAEKTIVNIQRTAHQWHEFYIHVAATTSSRKLETLHNALQQFAKKKDKPEGLYPRMGFSLTGIEDSTRLSIRIVFRQRGNWQSMEKKWACQSMCTWAIKNACDTIGITYYLPEVPVVMKNKM 1165          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: D7FX37_ECTSI (Small Conductance Mechanosensitive Ion channel n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FX37_ECTSI)

HSP 1 Score: 442 bits (1137), Expect = 9.170e-137
Identity = 296/769 (38.49%), Postives = 409/769 (53.19%), Query Frame = 3

Query:  318 YFAVAMHYDVALLAWSVFLLVAWRRVLLQYIDQSGGNGSNVADA----VDNVLTSYVAFRVGWLVKDFAVLIIATKYLWRPYLERVQTNIFAQYVLLMLTDYVTKGYIDRDDERFALQISNKGMRWDR-LSLYAVSKAIGFIPRHKLGHEFFKDLLESNDTQLDSAKEARVLGNFLFEQLIFIA--------------------------------AGKSQRGGSRFNRYGRYGS-----------------------------------------VTGGN--------------GSGAGATLTPEDPLSRMAA----GNFGATGNTMFSV-----VGTEDNSAT------LRPPTAASVFAGHYLTI------------GRNALGRSP-LPTTSSRDSXXXXXXXTVXXXSXXASXXXXXXXXXSGSGIVPP-----IDPKERERDE--------------LAEVLTRVKH---LDQETFCPHLDPLLAEKAFAMFDIDGNGEATREEMVQGVVHTFHDHKSLSLTLKDSQHIAVKLGQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVMIHDDRG----KLQNCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALVGIEDSTKLTVRICFRQKSNFQDLSRYWAQHSICTQAIKEACDDLAITYSLPELPIGLKQK 2186
            YF VA+HYD+A+  WSV  +  WR +  Q++     + S +A A    V+  L  ++A RVG L+K++ VL++AT YLWRPYL+RVQ++I AQY+LL+LTDY TKGY +  D+ FA+++  +G++  + +SLYAVSKA+GFIPR+KLGH FF+ L  SN   LDS+K+A  LGNFLF+QLI  +                                +  S  G   +    R+G                                            GG                S A +TL+   P++  AA    G  GA G T  S      VGT  ++ T      +R   A +   G  L+I                 G SP  P  S   S            +   S         S S          +D + R++D+               AE   + K    L + TFCP L+P L + AF +FD+D +G  T+EEMV GVV TF DH+SL+ TL+DS+HIA KLG II  VI         ++WG DV +LS+TFASFL+A +FMIG AASNL  +++++FV R+YDVGDRV I+        +  N  VVK++L+ T FKRWD+Q FY+PN +L+T+T +N+QR+ HQWHEF IQVAA TT E+L  L+  LQEF+++ D P+ LY  M F+LV IEDSTKLT+RI FRQ+ N+Q++ + WA  S+CT AIK ACD L I+Y LPE+P+ +K+K
Sbjct:  368 YFVVALHYDIAVTIWSVVAMYIWRELFHQWVYT---DDSKLARAIFRHVNPALECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNT--LDSSKDATTLGNFLFDQLIIRSRPPPAXXXXXXXXXXXXXXXXXXXXXXGSIRMSRGSHAGAGTYRALPRFGGQMQKRNSEPMAVPASPRTTVASPKRFSMFEAGRPQVEGMPLANGGXXXXXAGKVPVAKAVSSKASSTLSKLVPVAGRAALVSSGRGGAGGGTESSDARCDDVGTTSSANTSPGRSRVRRRKAGTCVDGVDLSIEVASGGETEATGDTGTEGESPEQPVASKSPSGRDTRIDRARILAQNRSVFTSCSSVASSSNYGDKECGDGVDGEGRQKDQEDTVKGQAARSGETKAEKKEKPKQEEVLVRTTFCPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKK 1131          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: A0A6H5JDS1_9PHAE (EF-hand domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JDS1_9PHAE)

HSP 1 Score: 403 bits (1036), Expect = 5.350e-122
Identity = 304/875 (34.74%), Postives = 429/875 (49.03%), Query Frame = 3

Query:   18 EEDLMIGEIKTVKLGREARALGRYICYSLWLIVPCLTVHLLHKLGGIDNHYSVATVSVLYWSIFFSAALLSLPFLRLTARTFAYCAMRYGSKLLYRSNTLYFAVAMHYDVALLAWSVFLLVAWRRVLLQYIDQSGGN-GSNVADAVDNVLTSYVAFRVGWLVKDFAVLIIATKYLWRPYLERVQTNIFAQYVLLMLTDYVTKGYIDRDDERFALQISNKGMRWDR-LSLYAVSKAIGFIPRHKLGHEFFKDLLESNDTQLDSAKEARVLGNFLFEQLI------------------------FIAAG----KSQRG----GSRFNRYGRYG------------------------------------------SVTGGNGSGAG-------------ATLTPEDPL----SRMAAGNFGATGNTMFSVVGTEDNSATLRPPT-----------AASVFAGHYLTIGRNALGR---------------SPLPTTS--SRDSXXXXXXXTVXXXSXXASXXXXXXXXXSGSGIVPP-IDPKERERD-----------------ELAEVLTRVKHLDQETFCPHLDPLLAEKAFAMFDIDGNGEATREEMVQGVVHTFHDHKSLSLTLKDSQHIAVKLGQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVMIH---DDRGKLQ-NCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALVGIEDSTKLTVRICFRQKSNFQ---------DLSRYWAQHSICTQAIKEACDDLAITYSLPELPIGLKQK 2186
            E +LMIGEI++++L  E   + R I   +  +V  + V      G +D   ++  V       F S  LLS P +R TAR FA+      S LLYR N  YF VA+HYD+A+  WSV     W+ +  +++          +   V  VL  ++A RVG L+K++ VL++AT YLWRPYL+RVQ++I AQY+LL+LTDY TKGY +  D+ FA+++  +G++  + +SLYAVSKA+GFIPR+KLGH FF+ L  SN   LDS+K+A  LGNFLF+QLI                        F  AG    +  RG    G  +    R+G                                          ++ GG G+ AG             +TL+   P+    S M++G   A+G T  S V   D   T    T           A +   G  L+I   + G                 P+ +TS   RD+            +  +S          G       +D + R++D                 E  E   + + L ++TFCP L+P L + AF +FD+D +G  T+EEMV GVV TF DH+SL+ TL+DS+HIA KLG II  VI         ++WG DV +LS+TFASFL+A +FMIG AASNL  +++++FV R+YDVGDRV I+   + RG+   N  VVK++L+ T FKRWD+Q                                            A LQEF+++ D P+ LY  M F+LV IEDSTKLT+RI FRQ+ N+Q         ++ + WA  S+CT AIK ACD L I+Y LPE+P+ +K+K
Sbjct:  323 EPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVR--EFAGELDKDVNIREVV-----FFVSLLLLSFPVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVARYIWKELFHEWVYTDDSKMARTIFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNT--LDSSKDATTLGNFLFDQLIIRSRPPPARPTTKTSPPRKSLAAAFTGAGGGSIRMSRGSHGVGGTYRALPRFGGQMQKRNSESMAVPASPRTPVASPKRFSTFDAGRPQVEGMPLAIGGGGGAAAGKVPVAKAVSSKASSTLSKLVPVAGRASLMSSGRGSASGGTESSDVPGGDAGTTSSANTSPGRSRGRRREARTCVNGVGLSIADGSGGEMEATGETGTEGESPEQPVVSTSPSGRDTRIDRARVLAQNRNVFSSCSSVANSSNYGDKECGDGVDGEGRQQDQEXXXXXXXXXXXXTEAEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNSGTITKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTRGEEPTNVTVVKVDLMTTVFKRWDEQ--------------------------------------------ASLQEFSKSKDKPEGLYPRMGFSLVRIEDSTKLTIRITFRQRGNWQVGQEAIVVVNMEKKWACQSMCTWAIKSACDSLNISYFLPEIPVQMKEK 1144          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: A0A835Z0M4_9STRA (Mechanosensitive ion channel-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z0M4_9STRA)

HSP 1 Score: 310 bits (793), Expect = 1.820e-88
Identity = 243/728 (33.38%), Postives = 358/728 (49.18%), Query Frame = 3

Query:  234 PFLRLTARTFAYCAMRYGSKLLYRSNTLYFAVAMHYDVALLAWSVFLLVAWRRVLLQYIDQSGGNGSNVADAVDNVLTSYVAFRVGWLVKDFAVLIIATKYLWRPYLERVQTNIFAQYVLLMLTDYVTKGYIDRDDERFALQISNKGMRWDRLSLYAVSKAIGFIPRHKLGHEFFKDLL-----ESNDTQLDSAKEARVLGNFLFEQLIFIAAGKSQRGGS---------------RFNRYGRYGSVTGGNGSGAGATLTPED------------------------------------PLSRMAAGNFGATGN--TMFSVVGTEDNSATLRPP---------------TAAS-----------VFAGHYLTIGRNALGRSPLPTTSSRDSXXXXXXXTVXXXSXXASXXXXXXXXXSGSGIVPPIDPKERERDELAEVLTRVKHLDQETFCPHLDPLLAEKAFAMFDIDGNGEATREEMVQGVVHTFHDHKSLSLTLKDSQHIAVKLGQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVMIHD--DRGKLQNCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALVGIEDSTKLTVRICFRQKSNFQDLSRYWAQHSICTQAIKEACDDLAITYSLP 2159
            P     AR FAY ++   S++L+R N LYF VA+H ++  +     LL  W + +   +     +      AV N +   +A  V   +++F +L+IA+ +LW PYLER Q++IFAQYVLL+LT+Y + G I+ + E  A QI+ K  RW +LSLYAV K++ F+  HK+G   FK+L      +  +  +D++++AR LG +L+++L     G + R G+                   Y    S+  G+      T                                         PL R A+    A  +  +  S  GT+D+   L PP               TAA            +  G   ++ RN+   +P P       XXXXXX           XXXXXXXXX              E  EL  +       +   FCP +   L   AFA+FD + +G  T+++++QGVV  + DHK+L+ TL DSQ +  KLGQIIGA++         ++WG+   ++SITF+SFL++  FM G+ A+ L  SII+VFV   YDVGDR+MI D     +   C VVKINLL T F+R D Q+  +PN +LS    IN+QRSG+ W EF + V+A T   +L  LK R+Q+FA  N++   +Y  + F    I+DS KL VRI  + + N+ D+ R W  H+   QAI   C +L IT++ P
Sbjct:  254 PLFNFLARLFAYLSITSTSRILFRFNALYFVVALHQELTNILVFGLLLAEWEQWIQPLVIDKAYS------AVHNTILCLLAAAVAVFLRNFTLLLIASTHLWLPYLERTQSSIFAQYVLLILTEYCSTGIINAEFEALASQIAAKTARWSKLSLYAVYKSVSFVQHHKVGGSVFKELGLNAEGDGGEDTIDNSRQARALGEYLYKRL-----GDAARAGTWQPPYKPXXXXXXXXPRRAYLPIQSLLHGHLGSKSTTACHHTSLQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPLRRTASEASLALSSPGSPRSRAGTKDDGPCLSPPPLLAQPLPLPLXPSVTAAPLQPQAPPPPSPILHGLLASLRRNSNVPAPQPAXXXXXXXXXXXXKQEGTPPTSPXXXXXXXXXXXXXXXXXXXXXMAAENAELPNI-------ELAKFCPAMSTTLRPHAFALFDKEQHGVVTKDDLLQGVVSIYKDHKNLARTLADSQQMVNKLGQIIGALMSFVMIFIWLSIWGYQFLSISITFSSFLLSFTFMFGSTATALVESIIFVFVTSAYDVGDRLMIPDIMQTSRYHGCKVVKINLLTTVFERGDGQSLIMPNKLLSQMALINLQRSGNMWFEFVVGVSATTPLHKLHSLKGRMQKFA--NEDSSGIYHKIGFTFTEIQDSAKLVVRINVQGRFNWGDVKRRWEVHNATCQAIAHHCAELGITFTPP 961          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: A0A835YNP7_9STRA (Mechanosensitive ion channel-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNP7_9STRA)

HSP 1 Score: 283 bits (723), Expect = 7.020e-86
Identity = 138/279 (49.46%), Postives = 202/279 (72.40%), Query Frame = 3

Query: 1362 EKAFAMFDIDGNGEATREEMVQGVVHTFHDHKSLSLTLKDSQHIAVKLGQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVM--IHDDRGKLQNCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALVGIEDSTKLTVRICFRQKSNFQDLSRYWAQHSICTQAIKEACDDLAITYSLPELPIGL-KQKL 2189
            E+ FA+FD D + + T EE+V GVV+ + DH+SL+LTL+DSQ +A KLG+++GA I          ++G D+ AL++TFAS LV ++ +IG A++NLF SI+++FV R +DVGDRV     D RG++QN VV +I+LL+T F+ WD++ +Y+PN +L+TR  IN+QRSG QWHEFFIQV A+T  ++L  LKA+  EFA  ++ P  ++ ++ FAL G+E++ +LT+R+CFRQK N+Q LSR WA  S+CT  I  +C +L ITY+ P+LP+   KQKL
Sbjct:    2 EQVFALFDRDDSNDITHEEVVNGVVNIYKDHQSLALTLQDSQRMAHKLGEVLGAGILIALIFIWLRIFGRDIFALTLTFASVLVGVSVLIGPASANLFASIVFIFVQRPFDVGDRVFHWFPDGRGEMQNAVVTRIDLLITCFRAWDEKVYYLPNHVLATRPIINIQRSGDQWHEFFIQVLASTPSQKLWELKAKCVEFAEKHNKPSGIHPHIQFALTGVENTNRLTIRVCFRQKGNWQILSRKWAVQSMCTNCIVNSCKELGITYTQPQLPLDFNKQKL 280          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: D7FX36_ECTSI (Small Conductance Mechanosensitive Ion channel n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FX36_ECTSI)

HSP 1 Score: 299 bits (765), Expect = 2.640e-84
Identity = 245/755 (32.45%), Postives = 359/755 (47.55%), Query Frame = 3

Query:  318 YFAVAMHYDVALLAWSVFLLVAWRRVLLQYIDQSGGNGSNVADAVDNVLTSYVAFRVGWLVKDFAVLIIATKYLWRPYLERVQTNIFAQYVLLMLTDYVTKGYIDRDDERFALQISNKGMR-WDRLSLYAVSKAIGFIPRHKLGHEFFKDLLESNDTQLDSAKEARVLGNFLFEQLIF----IAAGKSQRGGSRFNRYGRYGSVT-----GGNGSGA---------GATLTPEDPLSRMAAGNFGATGNTMFSVVGTEDNSATLRP-PTAASVFAGHYLTIG---RNALGRSPLPTTSSRDSXXXXXXXTVXXXSXXASXXXXXXXXXSGSGIVPPIDPKERERDELA--EVLT----------RVKH----------LDQETFCPHLDPLL------------AEKAFAMF---------------------------------DIDGNGEATREEMVQGVVHTFHDH----KSLSLTLK--DSQH-------------------------IAVKL------GQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVMIHDDRGKL----QNCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALVGIEDSTKLTVRICFRQKSNFQDLSRYWAQHSICTQAIKEACDDLAITYSLPELPIGLKQKL 2189
            YF VA+H D+++  WS+  +  WRR+  +++  +           D+ L+   A+R G    D                E  Q++I AQY+LL+LTDY T G  + DD++FA+++  +G++  +++SLYAVSKA+GFIPRHKLGH FF++L  +N   L+S+++A+ LG ++F+QL+      + G +        R+    +V      GG GS A         G  L      S  ++   G  G+   S   + D  ATL+P P   S         G   R A GRSP+ T  S           +   +  AS         + +  V   D  +    E+   EVL           R  H           D ++F    DP +            AE  F+                                   D D  G +  E    G V   H+     +S   T K  DS                           +A K+      G I  A +         ++ GFDV +LS+TFASFL+A +FMIG AASNL  +++++FV R+YDVGDRV I+DD         N VVVK++L  T F+RWD+Q FYIPN +L+ +T +N+QR+ HQWHEF+I VAA T+ ++L  L   LQ+FA+  D P+ L+  M F+L GIEDST+L++RI FRQ+ N+Q++ + WA  S+CT AIK ACD + ITY LPE+PI +K K+
Sbjct:  318 YFVVALHQDISMTIWSIASMFVWRRLFAEWVYPT-----------DSELSGN-AYRYG----D----------------EFFQSSILAQYILLLLTDYATTGECNPDDQKFAIEMGRRGIKEQEKISLYAVSKAMGFIPRHKLGHAFFRELGGTNT--LNSSRDAKTLGEYMFDQLLLRSRMYSPGCTAPASPATRRWAYLANVGQTVLGGGGGSNANTLNAKSFRGGRLGASKRASLPSSWTAGDVGSLSPSKRTSSD--ATLKPAPWLRSRSGAGGRAAGSAPREAGGRSPIVTRVSSKLSSSVNKMRMGPNARAASDSAAG----AATATVDTADASDAGSSEIPMDEVLPSQGGGGMAGFREFHGGGKRALSVIEDADSFADS-DPFVGGESKAEVTASCAEMEFSSLRVVDAMAPRYGHDVSELAKETANGGSFSSEDRNDKDAGGASRGEGSAGGTVQKEHEESTNVQSNGSTQKGGDSDEADKPQEEELLTRETLCPMLEHQVLEMAFKIFDLNSNGSITKAFVWL-------SILGFDVLSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYDDVQTAGVEPMNVVVVKVDLRTTSFRRWDEQIFYIPNHLLADKTIVNIQRTAHQWHEFYIHVAATTSSQKLETLHDALQKFAKKKDKPEGLHPRMGFSLTGIEDSTRLSIRIIFRQRGNWQNMDKKWACQSMCTWAIKNACDTIGITYYLPEVPIVMKNKI 1024          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: A0A835YTT2_9STRA (EF-hand domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YTT2_9STRA)

HSP 1 Score: 183 bits (465), Expect = 3.820e-45
Identity = 189/676 (27.96%), Postives = 293/676 (43.34%), Query Frame = 3

Query:  198 WSIFFSAALLSLPFLRLTARTFAYCAMRYGSKLLYRSNTLYFAVAMHYDVALLAWSVFLLVAWRRVLLQYIDQSGGNGSNVADAVDNVLTSYVAFRVGWLVKDFAVLIIATKYLWRPYLERVQTNIFAQYVLLMLTDYVTKGY-IDRDDERFALQISNKGMRWDRLSLYAVSKAIGFIPRHKLGHEFFKDLLESNDTQLDSAKEARVLGNFLFEQLIFIAAGKSQRGGSRFNRYGRYGSVTGGNGSGAGATLT-------PEDPLSR--MAAGNF---GATGNTMFSVVGTEDNSATLRPPTAASVFAGHYLTIGRNALGRSPLPTTSSRDSXXXXXXXTVXXXSXXASXXXXXXXXXSGSGIVPPIDPKERERDELAEVLTRVKHLDQETFCPHLDPLLAEKAFAMFDIDGNGEATREEMVQGVVHTFHDHKSLSLTLKDSQHIAVKLGQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVMIHDDRGKLQNCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALVGIE--DSTKLTVRICFRQKSNFQDLSRYWAQHSICTQAIKEACDDLAITYSLPELPIGLK 2180
            W++    A+ + P L+L  R       R  ++L++ ++  YFA+ +   VA   W++ L + W+R++   +   GG GS    A + +L  ++AFR  WLVKDF V  +A + LW PYL RVQ +IFAQYV++ML+++   G      D+  ALQ +     W  +SLYA+S+A+ F+P  +L          S    + S + AR     LF  +I   A + ++G                                 P  PL++  +  G     G+ G T+ S              T+  V +G                       XXXXXXX  XXX X AS          G+ +     P       LA VLT        +  P +   LA K   + D + +   T +E V  +V  +   +SLSL+L+D + I  KL  ++              ++G  V  L +++ S L+A +F+   +A  L  S +++FV R +DVGD V   DD G+    VV +INLL T  KRWD Q    PN  L+ R   N++RSG Q +   + V    + E L   +  L+  ARA     +L    I A    +    T L V + + Q  ++QD +  W  H+   +        LA+   LP  P+ L+
Sbjct:   35 WAVAAFVAVAAYPSLQLAVRAAGAAIAR--TRLVHVTHV-YFALRLARRVAAFLWALLLFLLWQRLVAPRVRGLGGGGS----AFERLLACHLAFRTIWLVKDFVVQEVAHRTLWAPYLARVQASIFAQYVIMMLSEFAMDGRRCGAVDQAVALQYAANEANWHNMSLYAISQAMSFVPSQRL----------SGAAAIQSKRTAREFAAVLFRSVIAQCA-RPRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERAPPTPPLAQWQVVRGLLRVDGSGGGTVKSA------------RTSGDVGSGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRAS----------GTDV-----PSGDAAAALAGVLTL------RSLRPAMCKPLARKTLELLDANADATITEDEFVSAIVRVYQKRRSLSLSLQDYEIILRKLSYMLSVGFAVFLLFVCLWIFGQGVVGLGVSWVSLLLAGSFVFSTSAQGLCESFVFIFVTRPFDVGDGVHFRDDMGQDNVFVVHRINLLSTVLKRWDGQYVVWPNRFLALREIRNMRRSGPQSNTLTVFVRRDASAEALARYRDALRAAARAAPRDHALVPDSIGAAFRADAGGGTALEVCVSYTQVQSWQDANERWRTHAFLARVALTLRASLALAPDLPTQPVLLR 659          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: A0A835YNU8_9STRA (Mechanosensitive ion channel-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNU8_9STRA)

HSP 1 Score: 162 bits (409), Expect = 2.930e-37
Identity = 102/286 (35.66%), Postives = 161/286 (56.29%), Query Frame = 3

Query:    6 DDDDEEDLM---IGEIKTVKLGREARALGRYICYSLWLIVPCLTVHLLHKLGGIDNHYSVATVSVLYWSIFFSAALLSLPFLRLTARTFAY--CAMRYGSKLLYRSNTLYFAVAMHYDVALLAWSVFLLVAWRRVLLQYIDQSGGNGSNVADAVDNVLTSYVAFRVGWLVKDFAVLIIATKYLWRPYLERVQTNIFAQYVLLMLTDYVTKGYIDRDDERF-ALQISNKGMRWDRLSLYAVSKAIGFIPRHKLGHEFFKDLLESNDTQLDSAKEARVLGNFLFEQLI 845
            DD+D E+ +   +GE+  + L +EAR L +Y+  +L +  P   +  +H  G I     V    +  W+     A+ S PF  +  +T     C  R+       SN +YF V +HY  A L W+VF+ + W+  L +Y  +S     +   AV  VL  Y+  R+ +L+   ++L +A +Y+WRPYLERVQ NIFAQYV+L+LTDY + GY+  D +++ ALQ S     WD++S+YA+S+A+GFIP++KL H  F     +    +DS + AR+    LF++L+
Sbjct:  289 DDEDSENAVFEHLGELPMMTLRKEARFLVKYLLIALAIFAPGAIIEKVH--GCI----LVRGQCLFVWASLAGMAVASYPFTAMAIKTLVMVLCNSRF-----IHSNHVYFFVRLHYASAQLMWAVFIYLFWKYGLAEY--KSPNMQESTDRAVGRVLACYMVSRIVYLLNKMSLLELANRYMWRPYLERVQANIFAQYVILILTDY-SLGYVRNDQDQYIALQFSENQGNWDKMSMYAISRAMGFIPKNKLRHPVFAG--SAGSVAMDSKRSARMFAGLLFDRLV 558          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: A9S7E3_PHYPA (MscS-Like mechanosensitive ion channel MSCL15 n=1 Tax=Physcomitrium patens TaxID=3218 RepID=A9S7E3_PHYPA)

HSP 1 Score: 143 bits (360), Expect = 5.740e-32
Identity = 87/289 (30.10%), Postives = 155/289 (53.63%), Query Frame = 3

Query: 1311 KHLDQETFCPHLDPLLAEKAFAMFDIDGNGEATREEMVQGVVHTFHDHKSLSLTLKDSQHIAVKLGQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVMIHDDRGKLQNCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALV--GIEDSTKLTVRICFRQKSNFQDLSRYWAQHSICTQAIKEACDDLAITYSLPELPI 2171
            K+L  + F   L    A KAF +F++   G  +++ ++Q VV  + + K+LSLTL D++ +  KL +++  ++          + G + + L + F+S L+   F+ GNAA + F S+I++F+M  +DVGDR+ + +      + VV ++N+L T F    ++  Y PN++L+++   N+ RS  QW     Q+ ++T  E++G LK R+ ++  +   PQ  Y Y  F LV   IEDS ++ + +  +   NFQ+    W + S     +K   +DL I + LP   I
Sbjct:  330 KYLTLDNFTEFLPEDKAAKAFGLFEVTDQGHISKKGLMQWVVSVYKERKALSLTLSDNRTVVAKLHRVLDVLMLAILLTICFLIMGVNTQKLLVAFSSILLPSVFVFGNAARSTFESLIFLFIMHPFDVGDRINVDN-----VSLVVEEMNILNTIFLSGSNEKVYYPNSVLASKPISNLYRSPDQWDAIEFQIHSSTPCEKIGILKERMTKYIESL--PQ--YWYPTFRLVCKDIEDSNRMKMALWMQHHMNFQESGERWQRRSNMILHMKTCMEDLKIGFMLPRQEI 609          

BLAST of mRNA_P-wetherbeei_contig656.5.2 vs. uniprot
Match: A0A2K1IAQ2_PHYPA (Mechanosensitive ion channel protein n=2 Tax=Physcomitrium patens TaxID=3218 RepID=A0A2K1IAQ2_PHYPA)

HSP 1 Score: 143 bits (360), Expect = 2.270e-31
Identity = 87/289 (30.10%), Postives = 155/289 (53.63%), Query Frame = 3

Query: 1311 KHLDQETFCPHLDPLLAEKAFAMFDIDGNGEATREEMVQGVVHTFHDHKSLSLTLKDSQHIAVKLGQIIGAVIXXXXXXXXXALWGFDVKALSITFASFLVAIAFMIGNAASNLFVSIIYVFVMRMYDVGDRVMIHDDRGKLQNCVVVKINLLLTEFKRWDDQTFYIPNAILSTRTAINVQRSGHQWHEFFIQVAAATTGERLGHLKARLQEFARANDNPQSLYGYMIFALV--GIEDSTKLTVRICFRQKSNFQDLSRYWAQHSICTQAIKEACDDLAITYSLPELPI 2171
            K+L  + F   L    A KAF +F++   G  +++ ++Q VV  + + K+LSLTL D++ +  KL +++  ++          + G + + L + F+S L+   F+ GNAA + F S+I++F+M  +DVGDR+ + +      + VV ++N+L T F    ++  Y PN++L+++   N+ RS  QW     Q+ ++T  E++G LK R+ ++  +   PQ  Y Y  F LV   IEDS ++ + +  +   NFQ+    W + S     +K   +DL I + LP   I
Sbjct:  712 KYLTLDNFTEFLPEDKAAKAFGLFEVTDQGHISKKGLMQWVVSVYKERKALSLTLSDNRTVVAKLHRVLDVLMLAILLTICFLIMGVNTQKLLVAFSSILLPSVFVFGNAARSTFESLIFLFIMHPFDVGDRINVDN-----VSLVVEEMNILNTIFLSGSNEKVYYPNSVLASKPISNLYRSPDQWDAIEFQIHSSTPCEKIGILKERMTKYIESL--PQ--YWYPTFRLVCKDIEDSNRMKMALWMQHHMNFQESGERWQRRSNMILHMKTCMEDLKIGFMLPRQEI 991