mRNA_P-wetherbeei_contig55234.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig55234.1.1
Unique NamemRNA_P-wetherbeei_contig55234.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: UPI0019C5B438 (2-oxo acid dehydrogenase subunit E2 n=1 Tax=Proteobacteria bacterium TaxID=1977087 RepID=UPI0019C5B438)

HSP 1 Score: 257 bits (656), Expect = 1.620e-84
Identity = 129/172 (75.00%), Postives = 147/172 (85.47%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIE 516
            AEHLAR+W TVP VLQA+EVDF  + E R AAG  WKARE  SLTYLPF+A AV  ALAK+P LNAS+DGD+L++HK IN+GIAVDLNFDGL+VPVVKD   KSLPQ+AR +NDLAQRAR+NRLKPD+LS  TYTLSNSGV+GT  TAPI+N PQVAILSTDGV+KKPVVIE
Sbjct:    2 AEHLARAWVTVPAVLQAIEVDFHTIDEVRRAAGGVWKAREGVSLTYLPFIARAVSIALAKYPRLNASVDGDELVVHKRINLGIAVDLNFDGLMVPVVKDVPSKSLPQIAREVNDLAQRARANRLKPDDLSEATYTLSNSGVFGTAFTAPIVNVPQVAILSTDGVQKKPVVIE 173
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A7C2UL63_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A7C2UL63_9PROT)

HSP 1 Score: 243 bits (619), Expect = 2.080e-76
Identity = 118/172 (68.60%), Postives = 141/172 (81.98%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIE 516
            AEH+ARSWTT PHV+QA+E DF RV EAR   G  WK+RE F+LTYLPF+A AV  ALAKFP LNA+ DG  L LH+ IN+GIAVDLNF+GL+VPVVKD   KSLPQ+AR I+DLA RAR+ RLKPD+L+  TYT++N+G +GTL TAPIIN PQVAILSTD ++K+PVVIE
Sbjct:  189 AEHMARSWTTAPHVMQAVEADFHRVVEARRRLGDQWKSREGFALTYLPFIARAVSVALAKFPNLNAAFDGTQLTLHRRINLGIAVDLNFEGLVVPVVKDVPSKSLPQVAREIHDLAARARAGRLKPDDLTEATYTITNNGAFGTLFTAPIINTPQVAILSTDAIRKRPVVIE 360
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A1F4DNB5_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_12_FULL_62_58 TaxID=1797497 RepID=A0A1F4DNB5_9PROT)

HSP 1 Score: 242 bits (617), Expect = 5.960e-76
Identity = 115/174 (66.09%), Postives = 145/174 (83.33%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIESD 522
            AEH+A++W +VPHVLQA+EVDF +V +AR  AG +WK RE FSLTYLPF+  AV  AL KFP LNA+  GD L++H+ +N+GIAVDLNF+GL+VPV+KD   KSLPQ+AR  NDL QRAR+NRLK DE++ GTYT+SN+G +GTL+TAPIIN PQVA+LSTD ++KKPVVIES+
Sbjct:  202 AEHMAKAWASVPHVLQAVEVDFHKVEQARRVAGEEWKRREGFSLTYLPFIVRAVSIALGKFPHLNATFAGDHLVVHRRVNLGIAVDLNFEGLVVPVLKDVPLKSLPQIAREANDLVQRARANRLKSDEMTEGTYTISNNGAFGTLMTAPIINWPQVAVLSTDAIQKKPVVIESE 375
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A0S8CA09_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Betaproteobacteria bacterium SG8_41 TaxID=1703391 RepID=A0A0S8CA09_9PROT)

HSP 1 Score: 241 bits (615), Expect = 9.060e-76
Identity = 116/174 (66.67%), Postives = 145/174 (83.33%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIESD 522
            AEH+A+SW+ VPHVLQA+E DF +V +AR  AG +WK RE FSLTYLPF+  +V  ALAKFP LNAS  GD L+L   IN+GIAVDLNF+GL+VPV+KD   KSLPQ+AR  N+L QRAR+NRLKPD+L+ GTYT++N+G +GTLITAPIINQPQVAILSTD ++K+P+VIE++
Sbjct:  192 AEHMAKSWSAVPHVLQAVEADFHKVEQARRVAGDNWKRREGFSLTYLPFILRSVSIALAKFPRLNASFAGDHLVLQPRINLGIAVDLNFEGLVVPVLKDVPQKSLPQIAREANELVQRARTNRLKPDDLTEGTYTITNNGAFGTLITAPIINQPQVAILSTDSIQKRPLVIETE 365
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A3N5WZE6_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A3N5WZE6_9PROT)

HSP 1 Score: 241 bits (615), Expect = 9.570e-76
Identity = 117/172 (68.02%), Postives = 140/172 (81.40%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIE 516
            AE++A+SW   PHVLQ +E DF RV  AR   G DWK RE F+LTYLPF+  AV  AL KFP LNASL GD L LH+ INIGIAVDLNFDGL+VPV+KD   KSLPQ+A+ INDLAQRAR+ +L+PDE++ GTYT++N+G +GT+ITAPIINQPQVAILS DG++KKPVVIE
Sbjct:  194 AENMAKSWHASPHVLQVVEADFHRVESARRLHGADWKRREGFALTYLPFIVRAVSIALGKFPKLNASLQGDGLALHRRINIGIAVDLNFDGLLVPVLKDVPAKSLPQIAKEINDLAQRARAGKLRPDEMTEGTYTITNNGAFGTVITAPIINQPQVAILSADGIRKKPVVIE 365
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A1F4BK48_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_02_FULL_67_26 TaxID=1797492 RepID=A0A1F4BK48_9PROT)

HSP 1 Score: 239 bits (610), Expect = 4.850e-75
Identity = 119/173 (68.79%), Postives = 141/173 (81.50%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIES 519
            AEH+A+SWTTVPHVLQ +E DF  V  AR AA   WK+RE FSLTYLPFVA AV   LAK+P LNAS  GD L LH+ +++GIAVDLNF+GL+VPVVKDAA KSL  LARAINDLA RAR NRLKPD+++  TYT++N+G YGTLITAP+I+ PQVAILSTD V+K+ VV+ES
Sbjct:  190 AEHMAKSWTTVPHVLQVVEADFSGVDRARRAAAAQWKSREGFSLTYLPFVAWAVTRGLAKYPRLNASFSGDSLALHRRVHLGIAVDLNFEGLLVPVVKDAAAKSLSDLARAINDLAVRARGNRLKPDDVTECTYTITNNGAYGTLITAPVISPPQVAILSTDAVRKRAVVVES 362
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A1F4AD98_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14 TaxID=1797482 RepID=A0A1F4AD98_9PROT)

HSP 1 Score: 236 bits (602), Expect = 1.100e-73
Identity = 114/173 (65.90%), Postives = 141/173 (81.50%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIES 519
            AEH+A+SWTT PHVLQA+EVDF RV +AR   G +WK RE F+LTYLPF+  AV  AL+KFP LN++ +GD L LH+ +N+GIAVDL  +GL+VPVVKD   KSL Q+AR INDLA RAR+N+LKPDE++ GTYT++N+G +GTL TAPIIN PQVAILSTD V+KKP V+ES
Sbjct:  203 AEHMAKSWTTTPHVLQAVEVDFHRVEQARQIHGAEWKQREGFALTYLPFIVRAVSIALSKFPNLNSTFNGDSLTLHRRVNLGIAVDLGGEGLVVPVVKDVPAKSLSQIAREINDLATRARANQLKPDEITEGTYTITNNGAFGTLFTAPIINPPQVAILSTDAVRKKPWVVES 375
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A2D8PRA0_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A2D8PRA0_9PROT)

HSP 1 Score: 234 bits (597), Expect = 4.380e-73
Identity = 115/174 (66.09%), Postives = 140/174 (80.46%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIESD 522
            AEH+ RS  T PHVLQA+E DF  V  AR A    WK RE FSLT+LPFVA AVC A+  FP +NA ++G+ L++H+ IN+GIAVDL+FDGL+ PVVKDA   SL +LARAI D++ RAR+N+L PD+ +GGTYT+SNSGV+GT+ITAPIINQPQVAILSTDGV KKPVVIES+
Sbjct:  191 AEHMVRSKATSPHVLQAVEADFASVGRARQAVSTQWKEREGFSLTWLPFVARAVCIAIRDFPRINAQIEGESLVVHRRINLGIAVDLDFDGLVAPVVKDADRMSLSELARAIRDVSSRARANKLTPDDFTGGTYTISNSGVFGTMITAPIINQPQVAILSTDGVTKKPVVIESE 364
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A1F4EY38_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_12_FULL_65_14 TaxID=1797501 RepID=A0A1F4EY38_9PROT)

HSP 1 Score: 230 bits (587), Expect = 1.830e-72
Identity = 114/171 (66.67%), Postives = 141/171 (82.46%), Query Frame = 1 
Query:    4 EHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIE 516
            EH+ RS  T PHVLQA+EVD++RV +AR A G +WK+RE FSLTYLPF+A AVC A+A FPL+NASL   +LI+HK +++GIAVDLNF+GL+VPVV+DA  + LP LAR +N LA+ AR   LKPDE++GGTYTLSNSG +GT +TA IINQPQVAILS DGV+KKPVV+E
Sbjct:  120 EHMLRSIATSPHVLQAVEVDYERVEQARNAKGGEWKSREGFSLTYLPFIARAVCEAIADFPLVNASLAEGELIVHKRVHLGIAVDLNFEGLVVPVVRDAQRRELPGLAREMNRLARAARDGTLKPDEVAGGTYTLSNSGSFGTYMTAAIINQPQVAILSCDGVRKKPVVLE 290
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BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Match: A0A520NRF2_9PROT (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A520NRF2_9PROT)

HSP 1 Score: 229 bits (585), Expect = 3.030e-71
Identity = 111/173 (64.16%), Postives = 138/173 (79.77%), Query Frame = 1 
Query:    1 AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFVAHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDAAGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPIINQPQVAILSTDGVKKKPVVIES 519
            AEH+ RS  T PHVLQA+E DF RV  AR      WK RE FSL YLPFVA AVC A+  FP +NA ++G+ L++H+ +N+GIAVDL+FDGL+ PVVKDA   SLPQLARAI ++++RAR+NRL PD+ + GTYT+SNSGV+GT+ITAPIINQPQVAILS DG+ KKPVV+E+
Sbjct:  194 AEHMVRSKATSPHVLQAVEADFGRVARARENTSRQWKEREGFSLNYLPFVARAVCTAIRDFPRVNAQIEGESLVVHRRVNLGIAVDLDFDGLVAPVVKDADRMSLPQLARAIREVSERARANRLTPDDFTEGTYTISNSGVFGTMITAPIINQPQVAILSIDGITKKPVVVEN 366
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig55234.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI0019C5B4381.620e-8475.002-oxo acid dehydrogenase subunit E2 n=1 Tax=Proteo... [more]
A0A7C2UL63_9PROT2.080e-7668.60Dihydrolipoamide acetyltransferase component of py... [more]
A0A1F4DNB5_9PROT5.960e-7666.09Dihydrolipoamide acetyltransferase component of py... [more]
A0A0S8CA09_9PROT9.060e-7666.67Dihydrolipoamide acetyltransferase component of py... [more]
A0A3N5WZE6_9PROT9.570e-7668.02Dihydrolipoamide acetyltransferase component of py... [more]
A0A1F4BK48_9PROT4.850e-7568.79Dihydrolipoamide acetyltransferase component of py... [more]
A0A1F4AD98_9PROT1.100e-7365.90Dihydrolipoamide acetyltransferase component of py... [more]
A0A2D8PRA0_9PROT4.380e-7366.09Dihydrolipoamide acetyltransferase component of py... [more]
A0A1F4EY38_9PROT1.830e-7266.67Dihydrolipoamide acetyltransferase component of py... [more]
A0A520NRF2_9PROT3.030e-7164.16Dihydrolipoamide acetyltransferase component of py... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig55234contigP-wetherbeei_contig55234:2..523 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog991905.SL003B_1038
PFAMs2-oxoacid_dh,Biotin_lipoyl,E3_binding
Model size522
Max annot lvl28211|Alphaproteobacteria
KEGG rclassRC00004,RC02727,RC02833
KEGG koko:K00658
KEGG ReactionR02570,R02571,R08549
KEGG Pathwayko00020,ko00310,ko01100,ko01110,ko01120,ko01130,ko01200,map00020,map00310,map01100,map01110,map01120,map01130,map01200
KEGG ModuleM00009,M00011,M00032
Hectar predicted targeting categoryother localisation
Exons1
Evalue5.36e-63
EggNOG OGsCOG0508@1|root,COG0508@2|Bacteria,1MUJD@1224|Proteobacteria,2TS5N@28211|Alphaproteobacteria,4BPQ0@82117|unclassified Alphaproteobacteria
EC2.3.1.61
DescriptionThe 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2)
Cds size522
COG categoryC
BRITEbr01601,ko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186823.800416-CDS-P-wetherbeei_contig55234:1..5231683186823.800416-CDS-P-wetherbeei_contig55234:1..523Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig55234 2..523 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig55234.1.1prot_P-wetherbeei_contig55234.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig55234 2..523 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig55234.1.1

>prot_P-wetherbeei_contig55234.1.1 ID=prot_P-wetherbeei_contig55234.1.1|Name=mRNA_P-wetherbeei_contig55234.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=174bp
AEHLARSWTTVPHVLQAMEVDFQRVYEARTAAGVDWKAREAFSLTYLPFV
AHAVCGALAKFPLLNASLDGDDLILHKSINIGIAVDLNFDGLIVPVVKDA
AGKSLPQLARAINDLAQRARSNRLKPDELSGGTYTLSNSGVYGTLITAPI
INQPQVAILSTDGVKKKPVVIESD
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mRNA from alignment at P-wetherbeei_contig55234:2..523+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig55234.1.1 ID=mRNA_P-wetherbeei_contig55234.1.1|Name=mRNA_P-wetherbeei_contig55234.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=522bp|location=Sequence derived from alignment at P-wetherbeei_contig55234:2..523+ (Phaeothamnion wetherbeei SAG_119_79)
GCGGAGCATCTCGCCAGGTCGTGGACAACGGTACCGCACGTGCTGCAGGC
CATGGAAGTCGATTTCCAGCGCGTATATGAGGCGCGGACCGCTGCTGGCG
TGGACTGGAAAGCGCGCGAAGCGTTTTCGCTGACCTACCTGCCGTTTGTC
GCGCATGCCGTATGCGGAGCCTTGGCGAAATTCCCGCTGCTCAACGCGAG
CCTCGATGGCGACGACTTGATATTGCACAAGTCCATCAATATCGGCATTG
CCGTCGATCTGAATTTCGACGGGCTGATCGTGCCGGTAGTGAAGGACGCG
GCCGGCAAGTCATTGCCGCAGCTTGCGCGCGCCATCAACGATCTGGCGCA
GCGCGCACGCAGCAACCGGCTGAAACCCGACGAGTTGAGTGGCGGGACTT
ACACGCTGTCGAATTCGGGCGTATACGGTACGCTGATCACCGCACCCATC
ATCAACCAGCCGCAGGTCGCGATCCTTTCGACGGACGGGGTGAAGAAGAA
GCCCGTCGTCATCGAAAGCGAC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig55234:2..523+

>mRNA_P-wetherbeei_contig55234.1.1 ID=mRNA_P-wetherbeei_contig55234.1.1|Name=mRNA_P-wetherbeei_contig55234.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=522bp|location=Sequence derived from alignment at P-wetherbeei_contig55234:2..523+ (Phaeothamnion wetherbeei SAG_119_79)
GCGGAGCATCTCGCCAGGTCGTGGACAACGGTACCGCACGTGCTGCAGGC
CATGGAAGTCGATTTCCAGCGCGTATATGAGGCGCGGACCGCTGCTGGCG
TGGACTGGAAAGCGCGCGAAGCGTTTTCGCTGACCTACCTGCCGTTTGTC
GCGCATGCCGTATGCGGAGCCTTGGCGAAATTCCCGCTGCTCAACGCGAG
CCTCGATGGCGACGACTTGATATTGCACAAGTCCATCAATATCGGCATTG
CCGTCGATCTGAATTTCGACGGGCTGATCGTGCCGGTAGTGAAGGACGCG
GCCGGCAAGTCATTGCCGCAGCTTGCGCGCGCCATCAACGATCTGGCGCA
GCGCGCACGCAGCAACCGGCTGAAACCCGACGAGTTGAGTGGCGGGACTT
ACACGCTGTCGAATTCGGGCGTATACGGTACGCTGATCACCGCACCCATC
ATCAACCAGCCGCAGGTCGCGATCCTTTCGACGGACGGGGTGAAGAAGAA
GCCCGTCGTCATCGAAAGCGAC
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