mRNA_P-wetherbeei_contig55121.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig55121.1.1
Unique NamemRNA_P-wetherbeei_contig55121.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: UPI0019CDEB65 (Polysaccharide deacetylase family protein n=1 Tax=Rhodoferax sp. TaxID=50421 RepID=UPI0019CDEB65)

HSP 1 Score: 333 bits (855), Expect = 1.280e-113
Identity = 154/174 (88.51%), Postives = 160/174 (91.95%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            DAM RC MRGSVSLNVA+CEHHPE+IK CAD+GWEFYSHGTYNTRYL  MDE QER+VIQDSIDTI KHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRL SVPYSLEMND I YNVNLV PRRY +ILKRQFDRLY EG +SGTVMCIPLHPYL
Sbjct:   77 DAMKRCNMRGSVSLNVAVCEHHPEVIKACADNGWEFYSHGTYNTRYLMGMDETQERSVIQDSIDTIHKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLTSVPYSLEMNDTIAYNVNLVQPRRYADILKRQFDRLYQEGEQSGTVMCIPLHPYL 250
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: UPI000A44DA43 (polysaccharide deacetylase family protein n=1 Tax=Limnohabitans sp. Rim11 TaxID=1100719 RepID=UPI000A44DA43)

HSP 1 Score: 328 bits (841), Expect = 2.340e-111
Identity = 151/174 (86.78%), Postives = 159/174 (91.38%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            D M RC MRGSVSLNVAMC+HHPE+IK CAD GWEFYSHGTYNTRYL  M+EAQER VIQDSIDTI KHTGQKLDGWLAPALTYT NT+DLVADMGLTYVCDLFHDDQPGPVKVKKG+L S+PYSLEMND IVYNVNLVSPRRYG+I+KRQFDRLY EG  SGTVMCIPLHPYL
Sbjct:   86 DVMKRCNMRGSVSLNVAMCDHHPEVIKACADEGWEFYSHGTYNTRYLMGMNEAQERQVIQDSIDTIYKHTGQKLDGWLAPALTYTPNTMDLVADMGLTYVCDLFHDDQPGPVKVKKGKLTSMPYSLEMNDTIVYNVNLVSPRRYGDIIKRQFDRLYEEGEHSGTVMCIPLHPYL 259
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: A0A848HED6_9BURK (Polysaccharide deacetylase family protein n=2 Tax=Ramlibacter agri TaxID=2728837 RepID=A0A848HED6_9BURK)

HSP 1 Score: 327 bits (838), Expect = 4.940e-111
Identity = 151/174 (86.78%), Postives = 159/174 (91.38%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            + M RC MRGSVSLNVAMCEHHPE+I  CA  GWEFYSHGTYNTRYL NM E QERAVIQDSIDTIRKHTGQKLDGWLAPALTYTD T+DLVA+MGLTYVCDLFHDDQPGPVKVK+G+L S+PYSLEMNDVIVYNVNLVSPRRY +ILKRQFDRLYAEG  SGTVMCIPLHPYL
Sbjct:   77 ETMKRCNMRGSVSLNVAMCEHHPEVIAACAKEGWEFYSHGTYNTRYLMNMTEEQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDRTMDLVAEMGLTYVCDLFHDDQPGPVKVKQGKLTSIPYSLEMNDVIVYNVNLVSPRRYADILKRQFDRLYAEGERSGTVMCIPLHPYL 250
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: A0A4Q6FHW3_9GAMM (Polysaccharide deacetylase n=1 Tax=Xanthomonadaceae bacterium TaxID=1926873 RepID=A0A4Q6FHW3_9GAMM)

HSP 1 Score: 325 bits (833), Expect = 2.850e-110
Identity = 148/174 (85.06%), Postives = 161/174 (92.53%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            + M RC MRGSVSLNVAMCEHHPE+I+ CA++GWEFYSHGTYNTRYL  M EAQERAVIQDSIDTI+KHTGQ+LDGWLAPALTYTD T+DLVA+MGLTYVCDLFHDDQPGPVK K GR+ S+PYSLEMND IVYNVNLVSPRRYG+ILKRQFDRLYAEG +SGTVMCIPLHPYL
Sbjct:   77 ETMKRCNMRGSVSLNVAMCEHHPEVIEACAENGWEFYSHGTYNTRYLMGMTEAQERAVIQDSIDTIKKHTGQRLDGWLAPALTYTDRTMDLVAEMGLTYVCDLFHDDQPGPVKTKTGRISSIPYSLEMNDTIVYNVNLVSPRRYGDILKRQFDRLYAEGEKSGTVMCIPLHPYL 250
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: A0A521YKB8_9PROT (Polysaccharide deacetylase n=2 Tax=unclassified Betaproteobacteria TaxID=33809 RepID=A0A521YKB8_9PROT)

HSP 1 Score: 325 bits (832), Expect = 3.910e-110
Identity = 145/174 (83.33%), Postives = 162/174 (93.10%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            +AMDRCGMRGSVSLNVA+C+HHPE+I+ C   GWE YSHGTYNTRYLF M EAQERA+IQDSID +RKH+GQKLDGWLAPALTYT+ TLDLVA+MGLTYVCDLFHDDQPGPVKV+ GRL S+PYSLEMNDVI YN+NLVSPRRYG+I++RQFDRLYAEGA+SGTVMCIPLHPYL
Sbjct:   77 EAMDRCGMRGSVSLNVAVCDHHPEVIQACVQRGWELYSHGTYNTRYLFEMSEAQERALIQDSIDAVRKHSGQKLDGWLAPALTYTERTLDLVAEMGLTYVCDLFHDDQPGPVKVRDGRLASIPYSLEMNDVIAYNINLVSPRRYGDIIRRQFDRLYAEGADSGTVMCIPLHPYL 250
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: A0A1Q8IYJ2_9BURK (Polysaccharide deacetylase n=8 Tax=Burkholderiales TaxID=80840 RepID=A0A1Q8IYJ2_9BURK)

HSP 1 Score: 324 bits (830), Expect = 8.140e-110
Identity = 148/174 (85.06%), Postives = 157/174 (90.23%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            D M RC MRGSVSLNVAMCEHHPE+I  CA++GWEFYSHGTYNTRY+  M E QERAVIQDSIDTIRKHTGQKLDGWL+PALTYTD TLDLVA+MGLTYVCDLFHDDQPGPVK  KGRL S+PYSLEMND IVYNVN VSPRRYG+I+KRQFDRLY EG ESGTVMCIPLHPYL
Sbjct:   77 DVMKRCNMRGSVSLNVAMCEHHPEVIAACAENGWEFYSHGTYNTRYMMGMSEEQERAVIQDSIDTIRKHTGQKLDGWLSPALTYTDRTLDLVAEMGLTYVCDLFHDDQPGPVKTSKGRLASIPYSLEMNDTIVYNVNQVSPRRYGDIIKRQFDRLYREGEESGTVMCIPLHPYL 250
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: A0A4P5U0J0_9BURK (Polysaccharide deacetylase n=1 Tax=Comamonadaceae bacterium TaxID=1871071 RepID=A0A4P5U0J0_9BURK)

HSP 1 Score: 322 bits (825), Expect = 4.690e-109
Identity = 147/174 (84.48%), Postives = 160/174 (91.95%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            +AM RC MRGSVSLNVAMC+HHPE+I+ CAD GWEFYSHGTYNTRYL  M EAQERAVIQDSIDT+R++TGQKLDGWLAPALTYT+ TLDLVA+MGLTYVCDLFHDDQPGPVKV++GRL SVPYSLEMND IVYNVNLV PRRY +ILKRQFDRLY EG +SGTVMCIPLHPYL
Sbjct:   77 EAMQRCNMRGSVSLNVAMCQHHPEVIQACADKGWEFYSHGTYNTRYLMGMTEAQERAVIQDSIDTVRQYTGQKLDGWLAPALTYTERTLDLVAEMGLTYVCDLFHDDQPGPVKVRQGRLTSVPYSLEMNDTIVYNVNLVQPRRYADILKRQFDRLYEEGEQSGTVMCIPLHPYL 250
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: A0A1F3YAD8_9PROT (Polysaccharide deacetylase n=1 Tax=Betaproteobacteria bacterium RBG_16_66_20 TaxID=1797479 RepID=A0A1F3YAD8_9PROT)

HSP 1 Score: 321 bits (822), Expect = 1.300e-108
Identity = 145/174 (83.33%), Postives = 160/174 (91.95%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            + MDRCGMRGSVSLNVA+CEHHPE+I  C + GWE YSHGTYNTRYLFN+ E QERAVIQDSIDTIR+ +GQKLDGWLAPALTYT+ TLDLVA+MGL+YVCDLFHDDQPGP+KVKKGRL S+PYSLEMNDVI YNVNLV+PR YGEI++RQFDRLYAEG ESGTVMCIPLHPYL
Sbjct:   77 EVMDRCGMRGSVSLNVAVCEHHPEVIAACVERGWELYSHGTYNTRYLFNLSEDQERAVIQDSIDTIRRASGQKLDGWLAPALTYTERTLDLVAEMGLSYVCDLFHDDQPGPIKVKKGRLASIPYSLEMNDVIAYNVNLVAPRHYGEIIRRQFDRLYAEGEESGTVMCIPLHPYL 250
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: A0A177QUM7_9PROT (Polysaccharide deacetylase (Fragment) n=1 Tax=Betaproteobacteria bacterium SCGC AG-212-J23 TaxID=1799662 RepID=A0A177QUM7_9PROT)

HSP 1 Score: 307 bits (787), Expect = 1.700e-103
Identity = 138/174 (79.31%), Postives = 157/174 (90.23%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            +AMDRCG+RGSVSLNVA+CEHHPE+I+ C + GWE YSHGTYNTRY F M EAQER +IQDSIDTI++ +GQKLDGWLAPALTYT+ TLDLVA+MGLTYVCDLFHDDQPGPVKVK+GRL S+PYSLEMND I +NV+ V+PR YGEI++RQFDRLY EG ESGTVMCIPLHPYL
Sbjct:   63 EAMDRCGVRGSVSLNVAVCEHHPEVIEACVERGWELYSHGTYNTRYTFGMSEAQERELIQDSIDTIKRVSGQKLDGWLAPALTYTERTLDLVAEMGLTYVCDLFHDDQPGPVKVKQGRLASIPYSLEMNDAIAFNVSSVTPRHYGEIIRRQFDRLYEEGRESGTVMCIPLHPYL 236
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BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Match: UPI00199B544F (Polysaccharide deacetylase family protein n=1 Tax=Candidatus Parcubacteria bacterium TaxID=2762014 RepID=UPI00199B544F)

HSP 1 Score: 305 bits (781), Expect = 2.220e-102
Identity = 139/174 (79.89%), Postives = 155/174 (89.08%), Query Frame = 1 
Query:    1 DAMDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDEAQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCDLFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQFDRLYAEGAESGTVMCIPLHPYL 522
            +AMDRCG+R SVSLN A+CEHHPE++  C +  WE YSHGTYNTRY+FNM E QER +IQDSIDTIR+ +GQKLDGWLAPALTYTD TLDLVA+MGL+YVCDLFHDDQPG VKV+KGRL S+PYSLEMND I Y+VN VSPR YGEI+KRQFDRLYAEGAESGTVMCIPLHPYL
Sbjct:   77 EAMDRCGVRCSVSLNAAVCEHHPEVVAACVERDWELYSHGTYNTRYMFNMSETQERVLIQDSIDTIRRVSGQKLDGWLAPALTYTDRTLDLVAEMGLSYVCDLFHDDQPGVVKVRKGRLSSIPYSLEMNDAISYSVNWVSPRHYGEIIKRQFDRLYAEGAESGTVMCIPLHPYL 250
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig55121.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI0019CDEB651.280e-11388.51Polysaccharide deacetylase family protein n=1 Tax=... [more]
UPI000A44DA432.340e-11186.78polysaccharide deacetylase family protein n=1 Tax=... [more]
A0A848HED6_9BURK4.940e-11186.78Polysaccharide deacetylase family protein n=2 Tax=... [more]
A0A4Q6FHW3_9GAMM2.850e-11085.06Polysaccharide deacetylase n=1 Tax=Xanthomonadacea... [more]
A0A521YKB8_9PROT3.910e-11083.33Polysaccharide deacetylase n=2 Tax=unclassified Be... [more]
A0A1Q8IYJ2_9BURK8.140e-11085.06Polysaccharide deacetylase n=8 Tax=Burkholderiales... [more]
A0A4P5U0J0_9BURK4.690e-10984.48Polysaccharide deacetylase n=1 Tax=Comamonadaceae ... [more]
A0A1F3YAD8_9PROT1.300e-10883.33Polysaccharide deacetylase n=1 Tax=Betaproteobacte... [more]
A0A177QUM7_9PROT1.700e-10379.31Polysaccharide deacetylase (Fragment) n=1 Tax=Beta... [more]
UPI00199B544F2.220e-10279.89Polysaccharide deacetylase family protein n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig55121contigP-wetherbeei_contig55121:2..523 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog1220582.RRU01S_07_03760
PFAMsPolysacc_deac_1
Model size522
Max annot lvl28211|Alphaproteobacteria
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.22e-91
EggNOG OGsCOG0726@1|root,COG0726@2|Bacteria,1MV3V@1224|Proteobacteria,2U6K8@28211|Alphaproteobacteria,4BK6A@82115|Rhizobiaceae
DescriptionPolysaccharide deacetylase
Cds size516
COG categoryG
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186822.1839287-CDS-P-wetherbeei_contig55121:1..5171683186822.1839287-CDS-P-wetherbeei_contig55121:1..517Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig55121 2..517 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186822.1951995-UTR-P-wetherbeei_contig55121:517..5231683186822.1951995-UTR-P-wetherbeei_contig55121:517..523Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig55121 518..523 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig55121.1.1prot_P-wetherbeei_contig55121.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig55121 2..517 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig55121.1.1

>prot_P-wetherbeei_contig55121.1.1 ID=prot_P-wetherbeei_contig55121.1.1|Name=mRNA_P-wetherbeei_contig55121.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=172bp
MDRCGMRGSVSLNVAMCEHHPEIIKDCADSGWEFYSHGTYNTRYLFNMDE
AQERAVIQDSIDTIRKHTGQKLDGWLAPALTYTDNTLDLVADMGLTYVCD
LFHDDQPGPVKVKKGRLVSVPYSLEMNDVIVYNVNLVSPRRYGEILKRQF
DRLYAEGAESGTVMCIPLHPYL
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mRNA from alignment at P-wetherbeei_contig55121:2..523-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig55121.1.1 ID=mRNA_P-wetherbeei_contig55121.1.1|Name=mRNA_P-wetherbeei_contig55121.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=522bp|location=Sequence derived from alignment at P-wetherbeei_contig55121:2..523- (Phaeothamnion wetherbeei SAG_119_79)
GACGCGATGGACCGCTGCGGCATGCGTGGCAGCGTCTCGCTCAACGTGGC
GATGTGCGAGCACCATCCGGAGATAATCAAGGACTGCGCCGACAGCGGCT
GGGAGTTCTACAGCCACGGCACCTACAACACCCGCTACCTGTTCAACATG
GACGAGGCACAGGAACGCGCGGTGATCCAGGATTCCATCGACACGATCAG
GAAGCACACCGGCCAGAAGCTCGACGGCTGGCTGGCACCGGCGCTCACCT
ACACCGACAACACCCTCGACCTGGTGGCCGACATGGGCCTCACCTACGTG
TGCGACCTGTTCCACGACGACCAGCCCGGGCCGGTCAAGGTGAAGAAGGG
CCGCCTGGTATCGGTGCCCTACTCGCTGGAAATGAACGACGTGATCGTCT
ACAACGTCAACCTGGTGAGCCCCAGGCGCTATGGCGAGATCCTCAAACGC
CAGTTCGACCGGTTGTATGCCGAGGGTGCGGAATCCGGCACGGTGATGTG
CATCCCCCTGCACCCCTACCTG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig55121:2..523-

>mRNA_P-wetherbeei_contig55121.1.1 ID=mRNA_P-wetherbeei_contig55121.1.1|Name=mRNA_P-wetherbeei_contig55121.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=516bp|location=Sequence derived from alignment at P-wetherbeei_contig55121:2..523- (Phaeothamnion wetherbeei SAG_119_79)
ATGGACCGCTGCGGCATGCGTGGCAGCGTCTCGCTCAACGTGGCGATGTG
CGAGCACCATCCGGAGATAATCAAGGACTGCGCCGACAGCGGCTGGGAGT
TCTACAGCCACGGCACCTACAACACCCGCTACCTGTTCAACATGGACGAG
GCACAGGAACGCGCGGTGATCCAGGATTCCATCGACACGATCAGGAAGCA
CACCGGCCAGAAGCTCGACGGCTGGCTGGCACCGGCGCTCACCTACACCG
ACAACACCCTCGACCTGGTGGCCGACATGGGCCTCACCTACGTGTGCGAC
CTGTTCCACGACGACCAGCCCGGGCCGGTCAAGGTGAAGAAGGGCCGCCT
GGTATCGGTGCCCTACTCGCTGGAAATGAACGACGTGATCGTCTACAACG
TCAACCTGGTGAGCCCCAGGCGCTATGGCGAGATCCTCAAACGCCAGTTC
GACCGGTTGTATGCCGAGGGTGCGGAATCCGGCACGGTGATGTGCATCCC
CCTGCACCCCTACCTG
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