mRNA_P-wetherbeei_contig5510.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig5510.2.1
Unique NamemRNA_P-wetherbeei_contig5510.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: A0A7S2UU49_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UU49_9STRA)

HSP 1 Score: 125 bits (314), Expect = 1.160e-30
Identity = 68/151 (45.03%), Postives = 86/151 (56.95%), Query Frame = 1
Query:  253 SSVKQRYRTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLS-GPKKGRWGADEDALLVSLVGXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIRW 702
            SS+ +  R WT  ED  L  AV K G+ NW  IA  VG++N  QCLQRW++VL  G  KG+W A EDA LV LVG                          H LDP++KKG ++  ED  IL  Q E+GN+W+ IA+ +PGRTEN V+IRW
Sbjct:   21 SSIPKIPRKWTLVEDEELRHAVRKYGESNWKDIAREVGTRNHVQCLQRWKKVLKPGLVKGQWSALEDAKLVDLVGKGFKNWGQLAAQMPGRTSKQCRERWSHHLDPSVKKGEYTTEEDNTILRLQSELGNKWSEIAKHLPGRTENAVKIRW 171          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: A0A024UVV8_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UVV8_9STRA)

HSP 1 Score: 126 bits (317), Expect = 2.450e-30
Identity = 85/206 (41.26%), Postives = 105/206 (50.97%), Query Frame = 1
Query:   91 SMLVAKRTLPPRPTHAGVDHPSSSGFASGFRLEQVVPSPPERVRPTFPAGTDSSSSVKQRYRTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLS-GPKKGRWGADEDALLVSLV-GXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIRW 702
            S L+AKR    RPT    D P S G ++G R  QV P              DS   V+   R WTPE+D LL  A+E+ G +NW  IAE V  +N  QCLQRW +VL  G  KG W ADED  LV LV                            + LDP+I KG +S+ ED  + AA  E+GNRW  IA R+PGRTE+ V++RW
Sbjct:    2 SGLLAKRG---RPT----DDPESLGPSAGQRSRQVEP--------------DSEDGVQPSSR-WTPEQDELLRLAIEEFGSKNWKAIAERVPGRNHAQCLQRWNKVLKPGLVKGHWAADEDENLVRLVQNSTTVNWGEISKSIEGRTAKQCRERWKNHLDPSINKGPYSSDEDMLLAAAYAELGNRWTQIANRLPGRTEDSVKMRW 185          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: D8LM16_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LM16_ECTSI)

HSP 1 Score: 119 bits (298), Expect = 9.240e-29
Identity = 64/155 (41.29%), Postives = 85/155 (54.84%), Query Frame = 1
Query:  238 GTDSSSSVKQRYRTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLS-GPKKGRWGADEDALLVSLVGXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIR 699
            G  +      R R W P ED  L  AV++SG+++W  IA  VGS+N  QCLQRW++VL  G  KG+W A+EDA L+ L                            H LDP+I KG ++  ED  I+  Q E+GNRW+ IA R+PGRTEN ++IR
Sbjct:   95 GDPTRKKASTRPRRWQPAEDKKLTLAVKESGEKDWKSIALKVGSRNHVQCLQRWKKVLRPGLVKGQWDAEEDAALMKLATAPFKNWGVLSQSMSGRTSKQCRERWCHHLDPSINKGAYTEEEDNIIIETQAELGNRWSQIAARLPGRTENSIKIR 249          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: W4GLG9_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GLG9_9STRA)

HSP 1 Score: 121 bits (304), Expect = 1.580e-28
Identity = 78/206 (37.86%), Postives = 102/206 (49.51%), Query Frame = 1
Query:   91 SMLVAKRTLPPRPTHAGVDHPSSSGFASGFRLEQVVPSPPERVRPTFPAGTDSSSSVKQRYRTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLS-GPKKGRWGADEDALLVSLV-GXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIRW 702
            S L+ KR  P        D P S G ++G +  Q+ P              DS   +    R WTPE+D LL EA++  G +NW  IA+ V  +N  QCLQRW +VL  G  KG W ADED LL+ LV                            + LDP+I KG +S+ ED  + AA  E+GNRW  IA R+PGRTE+ V++RW
Sbjct:    2 SALLTKRGRPS-------DGPDSLGPSAGQKSRQLEP--------------DSEDGMLINTR-WTPEQDELLREAIDAFGSKNWKAIADRVPGRNHAQCLQRWNKVLKPGLIKGHWAADEDDLLIQLVQASTTVSWGELSKLIDGRTAKQCRERWKNHLDPSINKGSYSSEEDMLLTAAYAELGNRWTQIADRMPGRTEDSVKMRW 185          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: A0A6H5KJU6_9PHAE (MYB protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KJU6_9PHAE)

HSP 1 Score: 119 bits (298), Expect = 3.130e-27
Identity = 63/145 (43.45%), Postives = 84/145 (57.93%), Query Frame = 1
Query:  268 RYRTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLS-GPKKGRWGADEDALLVSLVGXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIR 699
            R R W PEED  L  AV++SG+++W  IA  VGS+N  QCLQRW++VL  G  KG+W A+EDA L+ L                            H LDP+I KG ++  ED  I+  Q ++GNRW+ IA R+PGRTEN ++IR
Sbjct:  112 RPRRWQPEEDKKLTLAVKESGEKDWKSIALKVGSRNHVQCLQRWKKVLRPGLVKGQWDAEEDAALMKLATAPFKNWGVLSQSMSGRTSKQCRERWCHHLDPSINKGAYTEEEDKIIIETQAKLGNRWSQIAARLPGRTENSIKIR 256          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: D8LSJ8_ECTSI (Myb-like DNA-binding protein, putative n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LSJ8_ECTSI)

HSP 1 Score: 112 bits (281), Expect = 6.690e-25
Identity = 63/156 (40.38%), Postives = 84/156 (53.85%), Query Frame = 1
Query:  238 GTDSSSSVKQRYRTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLS-GPKKGRWGADEDALLVSLVGXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIRW 702
            G D+    + +   W+ EED  L +AV +  +  W  IA  V ++N  QCLQRW++VL  G  KG+W A+ED  LV LV                           H LDPA++KG ++  ED  I+  Q EVGNRWA IA+R+ GRTEN V+IRW
Sbjct:   89 GLDNPRLKRNKPVKWSAEEDRRLRDAVVRFSEARWKDIASLVETRNHVQCLQRWKKVLKPGLVKGQWSAEEDDRLVGLVEKGFRNWGQVASFMDGRTSKQCRERWCHHLDPAVRKGGYTTEEDELIIFLQGEVGNRWADIAKRLTGRTENAVKIRW 244          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: A0A7S2WMH6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WMH6_9STRA)

HSP 1 Score: 103 bits (257), Expect = 8.510e-24
Identity = 64/173 (36.99%), Postives = 88/173 (50.87%), Query Frame = 1
Query:  190 QVVPSPPERVRPTFPAGTDSSSSVKQRYRTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLSGPK-KGRWGADEDALLVSLVGXXXXXXXXXXXXXXXXXXXXXXXXXXHR-LDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIRW 702
            Q +P PP++     P   D     K   R WT EED  L  AV ++G +NW +IAEA   + + QCL RW++VL+    KG W A+EDA ++ LV                           H  L+P I K  +S  ED  IL A  ++GNRWA IA+R+PGRT+N ++  W
Sbjct:    8 QPLPYPPQQHE--LPTLGDDQRKRKSCNRRWTTEEDEKLKIAVARNGAKNWKKIAEAFPDRTEVQCLHRWQKVLNPELVKGPWTAEEDAKVIELVKLHGPKKWTMIADCLPGRIGKQCRERWHNHLNPKISKLAWSEDEDRVILEAHEKLGNRWAEIAKRLPGRTDNAIKNHW 178          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: A0A2R5GXY0_9STRA (Transcription factor MYB3R-4 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GXY0_9STRA)

HSP 1 Score: 105 bits (261), Expect = 2.320e-22
Identity = 72/201 (35.82%), Postives = 97/201 (48.26%), Query Frame = 1
Query:  148 HPSSSGFASGFRLEQVVPSPPERVRPTFPAGTDSSS---SVKQRY-----------RTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLSGPK--KGRWGADEDALLVSLVGXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIRW 702
            H S+S F  G    +  P P      +   G DSS    +VK R            R WT +ED LL  AVE+   +NW  IA  V  ++  QCLQRW++ L  P+  KG W  +ED  L+ L                            + LDP+IKKG ++  ED  I+  Q E+GNRWAVI++ +PGRTEN V+IR+
Sbjct:  338 HESNSSFDGGSPSPEASPLPGSA---SSDYGGDSSRPDIAVKTREYARSEKPTAVPRRWTDDEDELLRRAVERHEKKNWKAIAAEVPGRSNVQCLQRWKKALD-PRVVKGHWTPEEDQRLLMLAAQNPKNWGYVAREIEGRNPKQCRERYTNHLDPSIKKGSWTPEEDQIIVEKQQELGNRWAVISKFLPGRTENSVKIRF 534          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: A0A835Z3R6_9STRA (Homeodomain-like protein (Fragment) n=2 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z3R6_9STRA)

HSP 1 Score: 98.2 bits (243), Expect = 3.680e-22
Identity = 64/154 (41.56%), Postives = 78/154 (50.65%), Query Frame = 1
Query:  259 VKQRY---RTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLSGP-KKGRWGADEDALLVSLV-GXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARR-VPGRTENHVRIRW 702
            V  RY   R W P+ED  L  AV+  G   W RIA  VG++N  QCLQRW++VL    KKG W  +EDALL   V                            H LDP IK+G F+AAEDA IL      GNRW  IAR+ +PGR +  +R R+
Sbjct:    2 VAPRYTPPRKWAPDEDERLNAAVQAHGATKWKRIAAQVGTRNHVQCLQRWKKVLRPDLKKGDWTPEEDALLTDTVQSGTCKNWGKVALHLPNRTYRQCQLRWQHHLDPDIKRGSFTAAEDALILEGFKRFGNRWKHIARKHLPGRIDYTIRGRY 155          
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Match: A0A5A8CX53_CAFRO (Uncharacterized protein n=4 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CX53_CAFRO)

HSP 1 Score: 103 bits (257), Expect = 1.070e-21
Identity = 60/157 (38.22%), Postives = 80/157 (50.96%), Query Frame = 1
Query:  232 PAGTDSSSSVKQRYRTWTPEEDVLLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLS-GPKKGRWGADEDALLVSLVGXXXXXXXXXXXXXXXXXXXXXXXXXXHRLDPAIKKGRFSAAEDAAILAAQVEVGNRWAVIARRVPGRTENHVRIR 699
            P   D   S   R R WT  ED  L  AV + G+R W  IAE V  ++  QCLQRWR+ L  G KKG W  +ED +L S V                           + LDP+IK+  ++A EDA +      +G++WA I+R++PGRTEN V+IR
Sbjct:   59 PHSGDHWDSAGTRPRKWTAAEDSALRSAVAEFGERRWKTIAERVPGRDHVQCLQRWRKALQPGLKKGHWSREEDEILKSEVACRPRNWGDVAIHIPGRTAKQCRERWSNHLDPSIKRSAWTAEEDALLERLVDAIGSKWASISRKLPGRTENAVKIR 215          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig5510.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S2UU49_9STRA1.160e-3045.03Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A024UVV8_9STRA2.450e-3041.26Uncharacterized protein n=1 Tax=Aphanomyces invada... [more]
D8LM16_ECTSI9.240e-2941.29Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
W4GLG9_9STRA1.580e-2837.86Uncharacterized protein n=1 Tax=Aphanomyces astaci... [more]
A0A6H5KJU6_9PHAE3.130e-2743.45MYB protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 Ta... [more]
D8LSJ8_ECTSI6.690e-2540.38Myb-like DNA-binding protein, putative n=2 Tax=Ect... [more]
A0A7S2WMH6_9STRA8.510e-2436.99Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
A0A2R5GXY0_9STRA2.320e-2235.82Transcription factor MYB3R-4 n=1 Tax=Hondaea ferme... [more]
A0A835Z3R6_9STRA3.680e-2241.56Homeodomain-like protein (Fragment) n=2 Tax=Tribon... [more]
A0A5A8CX53_CAFRO1.070e-2138.22Uncharacterized protein n=4 Tax=Cafeteria roenberg... [more]

Pages

back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig5510contigP-wetherbeei_contig5510:718..1587 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog157072.XP_008861199.1
PFAMsMyb_DNA-binding
Model size702
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons2
Evalue4.01e-31
EggNOG OGsCOG5147@1|root,KOG0048@2759|Eukaryota
DescriptionRNA polymerase II transcription regulator recruiting activity
Cds size702
COG categoryADK
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186821.9225621-CDS-P-wetherbeei_contig5510:717..11501683186821.9225621-CDS-P-wetherbeei_contig5510:717..1150Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig5510 718..1150 -
1683186821.935159-CDS-P-wetherbeei_contig5510:1318..15871683186821.935159-CDS-P-wetherbeei_contig5510:1318..1587Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig5510 1319..1587 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig5510.2.1prot_P-wetherbeei_contig5510.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig5510 718..1587 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig5510.2.1

>prot_P-wetherbeei_contig5510.2.1 ID=prot_P-wetherbeei_contig5510.2.1|Name=mRNA_P-wetherbeei_contig5510.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=234bp
MGNDFANPGSLLSDHGEAATHKFPCLASRGSMLVAKRTLPPRPTHAGVDH
PSSSGFASGFRLEQVVPSPPERVRPTFPAGTDSSSSVKQRYRTWTPEEDV
LLLEAVEKSGDRNWARIAEAVGSKNKTQCLQRWRRVLSGPKKGRWGADED
ALLVSLVGSGFDNWGQLAQRLPGRTAKQCRERWKHRLDPAIKKGRFSAAE
DAAILAAQVEVGNRWAVIARRVPGRTENHVRIRW
back to top

mRNA from alignment at P-wetherbeei_contig5510:718..1587-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig5510.2.1 ID=mRNA_P-wetherbeei_contig5510.2.1|Name=mRNA_P-wetherbeei_contig5510.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=870bp|location=Sequence derived from alignment at P-wetherbeei_contig5510:718..1587- (Phaeothamnion wetherbeei SAG_119_79)
ATGGGAAACGACTTTGCAAACCCAGGCTCACTTCTTTCTGACCATGGGGA AGCAGCCACACATAAATTTCCTTGTCTTGCAAGCCGAGGAAGCATGCTGG TGGCGAAGCGCACCCTCCCGCCGCGCCCTACCCACGCGGGTGTGGATCAT CCGTCGAGCAGCGGTTTCGCGAGTGGTTTCAGGCTTGAACAAGTCGTACC GAGCCCTCCCGAGCGTGTTCGGCCGACTTTCCCCGCCGGCACCGACTCCT CGTCGTCGGTCAAGCAAAGGTGAGGGCGAGGGCCGGGCCGATTTCTCGTT GTCGGAGGAATCAATCCGGGCACAACGCCCAACGGATACTCTCGGGAAAC ATTATAATTAGGACCACGGCGGCGTTCCTTTCTAAACTTACTCAGGCTTC CTAAAATGTCCACACTTGTGCGCATCCAATACCTCAGGTACCGAACATGG ACGCCCGAGGAGGATGTTCTGCTCCTCGAGGCGGTAGAGAAGTCCGGCGA CAGGAACTGGGCGCGCATCGCTGAAGCCGTCGGCTCAAAGAACAAGACTC AGTGCCTGCAGCGCTGGCGCCGCGTTTTGTCGGGCCCCAAGAAGGGCCGG TGGGGCGCCGACGAGGATGCGCTGCTCGTCTCGCTCGTCGGCAGCGGCTT TGACAACTGGGGTCAGCTGGCGCAGCGTCTGCCGGGGAGGACCGCGAAGC AGTGCCGCGAGCGCTGGAAGCATCGCCTGGACCCAGCGATCAAGAAGGGC CGCTTCTCGGCCGCTGAGGACGCCGCCATCCTTGCCGCCCAGGTCGAGGT CGGCAATCGCTGGGCCGTCATCGCGCGGCGCGTCCCCGGCCGCACCGAGA ACCATGTCCGCATCCGCTGG
back to top

Coding sequence (CDS) from alignment at P-wetherbeei_contig5510:718..1587-

>mRNA_P-wetherbeei_contig5510.2.1 ID=mRNA_P-wetherbeei_contig5510.2.1|Name=mRNA_P-wetherbeei_contig5510.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=702bp|location=Sequence derived from alignment at P-wetherbeei_contig5510:718..1587- (Phaeothamnion wetherbeei SAG_119_79)
ATGGGAAACGACTTTGCAAACCCAGGCTCACTTCTTTCTGACCATGGGGA
AGCAGCCACACATAAATTTCCTTGTCTTGCAAGCCGAGGAAGCATGCTGG
TGGCGAAGCGCACCCTCCCGCCGCGCCCTACCCACGCGGGTGTGGATCAT
CCGTCGAGCAGCGGTTTCGCGAGTGGTTTCAGGCTTGAACAAGTCGTACC
GAGCCCTCCCGAGCGTGTTCGGCCGACTTTCCCCGCCGGCACCGACTCCT
CGTCGTCGGTCAAGCAAAGGTACCGAACATGGACGCCCGAGGAGGATGTT
CTGCTCCTCGAGGCGGTAGAGAAGTCCGGCGACAGGAACTGGGCGCGCAT
CGCTGAAGCCGTCGGCTCAAAGAACAAGACTCAGTGCCTGCAGCGCTGGC
GCCGCGTTTTGTCGGGCCCCAAGAAGGGCCGGTGGGGCGCCGACGAGGAT
GCGCTGCTCGTCTCGCTCGTCGGCAGCGGCTTTGACAACTGGGGTCAGCT
GGCGCAGCGTCTGCCGGGGAGGACCGCGAAGCAGTGCCGCGAGCGCTGGA
AGCATCGCCTGGACCCAGCGATCAAGAAGGGCCGCTTCTCGGCCGCTGAG
GACGCCGCCATCCTTGCCGCCCAGGTCGAGGTCGGCAATCGCTGGGCCGT
CATCGCGCGGCGCGTCCCCGGCCGCACCGAGAACCATGTCCGCATCCGCT
GG
back to top