mRNA_P-wetherbeei_contig11949.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig11949.1.1
Unique NamemRNA_P-wetherbeei_contig11949.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: D7G5A8_ECTSI (Na_H_antiporter domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G5A8_ECTSI)

HSP 1 Score: 181 bits (459), Expect = 1.600e-49
Identity = 110/238 (46.22%), Postives = 143/238 (60.08%), Query Frame = 1
Query:   13 LEAPAVVHAGKDFAITVVLPPFLGGKPLPATIACKVWEEFSGESPDRHLMTTEIVVNGKLAHDFEIPPLQT-TLGAVFLRIDLGDKGKLGGIAHARVSCMPGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVLPAVQV 723
            +E   VVH    F+  V LPP           A  V  +  G   +      ++   G++  + ++  L   ++G   +R+ +G     G     RV+C+PG  SL PPLVTL +S+L RQV+VALLVG+WAGA ++    PL  F RTFDTYFV +F  EG+ +VLLFTFLLGGTIGLVQRSGGALG+A  LK FM  R    VC + L  ++FFDDYSSILIVG+SLR V+  V V
Sbjct:   27 VEVSPVVHVAAPFSALVRLPPSYAATGRTEAKASLV--DAQGGEKELAKQKLKVSAGGEVFLEHKLEGLLVGSIGTSTIRVRVG-----GETQDVRVTCVPGLVSLLPPLVTLAISILYRQVLVALLVGIWAGALLVSGFNPLGAFLRTFDTYFVGAFVGEGNAEVLLFTFLLGGTIGLVQRSGGALGLANALKGFMGTRTRGQVCTVTLGCLIFFDDYSSILIVGNSLRDVVRTVGV 257          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: A0A5D6Y930_9STRA (Na_H_antiporter domain-containing protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6Y930_9STRA)

HSP 1 Score: 152 bits (383), Expect = 1.130e-38
Identity = 104/254 (40.94%), Postives = 135/254 (53.15%), Query Frame = 1
Query:   10 RLEAPAVVHAGKDFAITVVLPPFLGGKPLPATIACKVWEEFSGESPDRHLMTT------------EIVVNGKLAHDFEIPPLQTTL--------GAVFLRIDLGDKGKLGGI-AHARVSCMPGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVL 708
            +L  P V+     FA+T+ LP    G   P  +A +V E+ SG         T            E+V   +L+  +    L TT+        GA            L  + A + V   P W SL PPLVTL++S LL QV+VALL GVW GATI+  G P TGF RTFD Y+VN+F  +GH  VLLFT +LGGTIG+VQ+ GG  G+A   K +M       +   LL  ++FFDDYS ILI+G+SLR VL
Sbjct:   25 QLLTPTVILQQVPFAVTIALPELTNGSVYPRRLAFRV-EDASGALLRNGTFETTAADGSFNSRFAEVVKKLQLSA-YGAQQLTTTVSELPEPAAGAXXXXXXXXXXXVLWSLSATSDVFVTPAWLSLLPPLVTLLMSALLSQVLVALLAGVWCGATIVAQGDPFTGFLRTFDQYWVNAFVDDGHAGVLLFTLVLGGTIGVVQKGGGGHGLALLAKEYMTSALRMQLSTWLLCLLIFFDDYSCILIIGNSLRQVL 276          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: A0A7S0H5F9_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0H5F9_9EUKA)

HSP 1 Score: 139 bits (350), Expect = 1.840e-37
Identity = 74/130 (56.92%), Postives = 92/130 (70.77%), Query Frame = 1
Query:  310 PGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLR 699
            P W SL PPL TLV S+ L+QV +A+L+G+W+GA ++  G PL  F RTFD Y V +F S  H  V+LFT LLGG IGLVQRSGGALG+A  ++RF   RR  ++  + L  +VFFDDYSSILIV SSL 
Sbjct:   73 PQWLSLLPPLATLVASIALKQVTLAMLIGIWSGALLLHGGNPLVAFLRTFDHYLVGAFLSAEHAGVVLFTLLLGGCIGLVQRSGGALGLAGLVQRFFDTRRKGLLSTIGLGGLVFFDDYSSILIVASSLE 202          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: A0A7S0P500_9EUKA (Hypothetical protein n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0P500_9EUKA)

HSP 1 Score: 147 bits (371), Expect = 3.670e-37
Identity = 78/137 (56.93%), Postives = 99/137 (72.26%), Query Frame = 1
Query:  313 GWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVLPAVQV 723
            GW S+ PP+V LV SV L+QVIVALL+GVWAG  ++  G  L   ARTFDTY V + A   H  VLLFTFLLGGTIGLVQ++GG L +   L+RFM+   +A+VC   +  ++FFDDYSS+LIVG+SL+  LPA+ V
Sbjct:   78 GWLSVLPPVVALVASVALKQVIVALLLGVWAGCLLLHKGNVLLATARTFDTYLVEAIADRAHAGVLLFTFLLGGTIGLVQKAGGGLALGLLLQRFMKSAHSALVCAWAMCHLIFFDDYSSLLIVGNSLQPALPALHV 214          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: A0A2D4CF21_PYTIN (Na_H_antiporter domain-containing protein (Fragment) n=2 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CF21_PYTIN)

HSP 1 Score: 148 bits (374), Expect = 3.710e-37
Identity = 77/139 (55.40%), Postives = 98/139 (70.50%), Query Frame = 1
Query:  292 ARVSCMPGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVL 708
            + V   PGW S+ PPLVTLV+S  L QV++ALL GVW GATI+  G PLTGF RTFDTY+V++F ++GH  VLLFT +LGG IG+VQ+ GG  G+A   K++M           +L  ++FFDDYS ILI+GSSLR VL
Sbjct:  637 SEVFVSPGWLSVLPPLVTLVISAALSQVLLALLAGVWCGATIVAQGNPLTGFLRTFDTYWVDAFVNDGHAGVLLFTIILGGAIGVVQKGGGGHGLALLAKQYMTTSMRMQFSTWMLCMLIFFDDYSCILIIGSSLRQVL 775          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: A0A7S4BES2_CHRCT (Hypothetical protein (Fragment) n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4BES2_CHRCT)

HSP 1 Score: 141 bits (356), Expect = 4.500e-37
Identity = 74/136 (54.41%), Postives = 99/136 (72.79%), Query Frame = 1
Query:  310 PGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVLPAV 717
            P W S+ PPLVTLV SV LRQVI+A+L+G+W+GA ++  G  L    RTFD YFV + AS  H  V+LFT +LGGTIGLVQ+SGGALG++  ++ F   RR  ++  + L +++FFDDYSSILIVG+SLR ++  V
Sbjct:   23 PRWLSVLPPLVTLVASVALRQVILAMLLGIWSGALLLHGGNALIAMLRTFDQYFVGALASSEHAGVVLFTLILGGTIGLVQKSGGALGLSSLVRGFFTTRRKGLLSTISLGSLIFFDDYSSILIVGNSLRPLISLV 158          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: K3WRN4_GLOUD (Na_H_antiporter domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WRN4_GLOUD)

HSP 1 Score: 147 bits (371), Expect = 5.680e-37
Identity = 75/133 (56.39%), Postives = 94/133 (70.68%), Query Frame = 1
Query:  310 PGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVL 708
            PGW SL PPLVTLV+S +L QV++ALL G+W GATI+  G P  GF RTFD Y+VN+F  +GH  VLLFT +LGGTIG+VQ+ GG  G+A   K +M       +   LL  ++FFDDYS ILI+G+SLR VL
Sbjct:  161 PGWLSLLPPLVTLVMSAVLSQVLIALLAGIWCGATIVAQGNPFVGFLRTFDQYWVNAFVDDGHAGVLLFTIILGGTIGVVQKGGGGHGLALLAKEYMTTSLRMQLSTWLLCLLIFFDDYSCILIIGNSLRQVL 293          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: M4C418_HYAAE (Na_H_antiporter domain-containing protein n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4C418_HYAAE)

HSP 1 Score: 146 bits (369), Expect = 9.460e-37
Identity = 78/137 (56.93%), Postives = 95/137 (69.34%), Query Frame = 1
Query:  298 VSCMPGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVL 708
            V+  PGW SL PPLVTL++S +L QV V+LL G+W GA I+  G P T F RTFD Y+VNSF +E H  VLLFT +LGGTIG+VQ+ GG  G+A   K+FM       +   LL  ++FFDDYS ILIVGSSLR VL
Sbjct:  138 VTASPGWVSLLPPLVTLIMSAVLGQVAVSLLAGIWCGAIIVSDGNPFTAFLRTFDYYWVNSFTAEDHAGVLLFTIILGGTIGVVQKGGGGHGLALIAKKFMTSSLRMQLSTWLLCLVIFFDDYSCILIVGSSLRQVL 274          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: A0A8K1CRC0_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CRC0_PYTOL)

HSP 1 Score: 146 bits (368), Expect = 1.270e-36
Identity = 76/138 (55.07%), Postives = 95/138 (68.84%), Query Frame = 1
Query:  295 RVSCMPGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVL 708
             V   PGW SL PPLVTLV+S    QV++ALL G+W GATI+  G PLTGF RTFD Y+V++F ++GH  VLLFT +LGG IG+VQ+ GG  G+A   K +M       +   LL  ++FFDDYS ILI+GSSLR VL
Sbjct:  131 EVFVTPGWLSLLPPLVTLVISATTNQVLIALLAGIWCGATIVAQGNPLTGFLRTFDQYWVDAFVNDGHAGVLLFTIILGGAIGVVQKGGGGHGLALLAKEYMTTSLRMQLSTWLLCLVIFFDDYSCILIIGSSLRQVL 268          
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Match: A0A7S3TUM3_EMIHU (Hypothetical protein (Fragment) n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A7S3TUM3_EMIHU)

HSP 1 Score: 140 bits (353), Expect = 1.280e-36
Identity = 78/149 (52.35%), Postives = 104/149 (69.80%), Query Frame = 1
Query:  277 GGIAHARVSCMPGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFARTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFMRGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVLPAVQV 723
            GG A A  +   GW SL PP+V L  SV LRQV++ALL+GVW+GA ++  G  L   ARTFD+YFV++ A   H  V+LFT LLGGTIGLVQ++GG L +A  L+RFM   R+ +  C  L +++FFDDYSS+LIVG+S+R  + A+ V
Sbjct:   61 GGDAAAAPNL--GWLSLIPPVVALSASVALRQVVLALLLGVWSGALLVHRGNGLLAAARTFDSYFVDALAEREHAGVVLFTLLLGGTIGLVQKAGGGLALAALLRRFMTSARSGLGACGALCSLIFFDDYSSVLIVGTSMREAIAALGV 207          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig11949.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G5A8_ECTSI1.600e-4946.22Na_H_antiporter domain-containing protein n=2 Tax=... [more]
A0A5D6Y930_9STRA1.130e-3840.94Na_H_antiporter domain-containing protein n=1 Tax=... [more]
A0A7S0H5F9_9EUKA1.840e-3756.92Hypothetical protein (Fragment) n=1 Tax=Phaeocysti... [more]
A0A7S0P500_9EUKA3.670e-3756.93Hypothetical protein n=1 Tax=Calcidiscus leptoporu... [more]
A0A2D4CF21_PYTIN3.710e-3755.40Na_H_antiporter domain-containing protein (Fragmen... [more]
A0A7S4BES2_CHRCT4.500e-3754.41Hypothetical protein (Fragment) n=1 Tax=Chrysotila... [more]
K3WRN4_GLOUD5.680e-3756.39Na_H_antiporter domain-containing protein n=1 Tax=... [more]
M4C418_HYAAE9.460e-3756.93Na_H_antiporter domain-containing protein n=1 Tax=... [more]
A0A8K1CRC0_PYTOL1.270e-3655.07Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A7S3TUM3_EMIHU1.280e-3652.35Hypothetical protein (Fragment) n=1 Tax=Emiliania ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig11949contigP-wetherbeei_contig11949:91..1127 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D7G5A8
PFAMsNa_H_antiporter
Model size723
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons2
Evalue2.63e-50
EggNOG OGsCOG1757@1|root,2QWQB@2759|Eukaryota
Ec32 ortholog descriptionNa /H antiporter, NhaC-like
Ec32 orthologEc-14_006840.1
DescriptionNa /H antiporter family
Cds size723
COG categoryC
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185932.5045457-CDS-P-wetherbeei_contig11949:90..3201683185932.5045457-CDS-P-wetherbeei_contig11949:90..320Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig11949 91..320 -
1683185932.5178244-CDS-P-wetherbeei_contig11949:634..11271683185932.5178244-CDS-P-wetherbeei_contig11949:634..1127Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig11949 635..1127 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig11949.1.1prot_P-wetherbeei_contig11949.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig11949 91..1127 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig11949.1.1

>prot_P-wetherbeei_contig11949.1.1 ID=prot_P-wetherbeei_contig11949.1.1|Name=mRNA_P-wetherbeei_contig11949.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=241bp
TQVRLEAPAVVHAGKDFAITVVLPPFLGGKPLPATIACKVWEEFSGESPD
RHLMTTEIVVNGKLAHDFEIPPLQTTLGAVFLRIDLGDKGKLGGIAHARV
SCMPGWASLFPPLVTLVLSVLLRQVIVALLVGVWAGATIICAGAPLTGFA
RTFDTYFVNSFASEGHVKVLLFTFLLGGTIGLVQRSGGALGMAQGLKRFM
RGRRNAMVCCLLLSAMVFFDDYSSILIVGSSLRAVLPAVQV
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mRNA from alignment at P-wetherbeei_contig11949:91..1127-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig11949.1.1 ID=mRNA_P-wetherbeei_contig11949.1.1|Name=mRNA_P-wetherbeei_contig11949.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1037bp|location=Sequence derived from alignment at P-wetherbeei_contig11949:91..1127- (Phaeothamnion wetherbeei SAG_119_79)
ACTCAGGTTCGCCTCGAGGCGCCTGCGGTGGTGCATGCCGGCAAGGATTT CGCTATCACCGTCGTCCTTCCACCCTTCCTAGGCGGCAAGCCATTGCCTG CAACCATTGCGTGCAAGGTCTGGGAAGAGTTCAGCGGAGAGAGCCCCGAT CGCCACCTCATGACGACGGAGATTGTGGTGAACGGCAAATTGGCGCACGA CTTTGAAATTCCGCCGCTCCAAACGACGCTCGGCGCTGTCTTCTTGCGAA TCGACCTCGGCGACAAGGGCAAGCTGGGAGGCATCGCGCATGCCCGCGTG TCGTGCATGCCAGGCTGGGCGTCTCTGTTTCCGCCGCTGGTGACGCTGGT GCTCTCCGTCTTGCTACGCCAGGTCATCGTGGCTCTTCTTGTGGGGGTGT GGGCCGGAGCCACAATCATCTGCGCCGGCGCTCCGCTCACCGGCTTCGCG CGCACGTTCGACACTTATTTCGTGAACTCCTTTGCGTCAGAAGGTGCGTT TGCTTTGCGTTCTATCCTCTTCTCTGTTTGCCCGTCAGCTGCCAGCGACG TGGAACTCAATCCTTTCCCTTTTTTTGCTGAGGCCAAAACTATTTTGACA ATATGTACCGTATTCAGGGAATATAAATTAGGTCTTGTTAGAGGACTGCC CGCTTCCTGTTGGTAAAACTAGTTGTCCTGCCACATGCATGCAGGCATTG TCGAGTTTATCGTCGCATCTTTCCTCTTGTTTGCGGGCTTCTCCTTCCCT GTAGGCACGCCAGCGGCCACCATCCTGCTTCTCTTCTTCTTTATCCTTCA TTTGCAGGCCACGTGAAGGTACTTCTTTTCACCTTCCTGCTGGGTGGAAC GATTGGGTTGGTGCAGCGCAGCGGTGGCGCCCTCGGTATGGCCCAGGGCC TGAAGCGCTTCATGCGGGGCCGCCGCAACGCCATGGTCTGCTGCTTGCTG CTGTCGGCGATGGTCTTCTTCGACGACTACTCCTCCATCCTCATCGTCGG CAGCTCGCTGCGTGCCGTCTTGCCCGCCGTTCAGGTG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig11949:91..1127-

>mRNA_P-wetherbeei_contig11949.1.1 ID=mRNA_P-wetherbeei_contig11949.1.1|Name=mRNA_P-wetherbeei_contig11949.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=723bp|location=Sequence derived from alignment at P-wetherbeei_contig11949:91..1127- (Phaeothamnion wetherbeei SAG_119_79)
ACTCAGGTTCGCCTCGAGGCGCCTGCGGTGGTGCATGCCGGCAAGGATTT
CGCTATCACCGTCGTCCTTCCACCCTTCCTAGGCGGCAAGCCATTGCCTG
CAACCATTGCGTGCAAGGTCTGGGAAGAGTTCAGCGGAGAGAGCCCCGAT
CGCCACCTCATGACGACGGAGATTGTGGTGAACGGCAAATTGGCGCACGA
CTTTGAAATTCCGCCGCTCCAAACGACGCTCGGCGCTGTCTTCTTGCGAA
TCGACCTCGGCGACAAGGGCAAGCTGGGAGGCATCGCGCATGCCCGCGTG
TCGTGCATGCCAGGCTGGGCGTCTCTGTTTCCGCCGCTGGTGACGCTGGT
GCTCTCCGTCTTGCTACGCCAGGTCATCGTGGCTCTTCTTGTGGGGGTGT
GGGCCGGAGCCACAATCATCTGCGCCGGCGCTCCGCTCACCGGCTTCGCG
CGCACGTTCGACACTTATTTCGTGAACTCCTTTGCGTCAGAAGGCCACGT
GAAGGTACTTCTTTTCACCTTCCTGCTGGGTGGAACGATTGGGTTGGTGC
AGCGCAGCGGTGGCGCCCTCGGTATGGCCCAGGGCCTGAAGCGCTTCATG
CGGGGCCGCCGCAACGCCATGGTCTGCTGCTTGCTGCTGTCGGCGATGGT
CTTCTTCGACGACTACTCCTCCATCCTCATCGTCGGCAGCTCGCTGCGTG
CCGTCTTGCCCGCCGTTCAGGTG
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