mRNA_P-wetherbeei_contig1784.3.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig1784.3.1
Unique NamemRNA_P-wetherbeei_contig1784.3.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: D7FXN4_ECTSI (Sar1A, Ras superfamily GTPase n=4 Tax=PX clade TaxID=569578 RepID=D7FXN4_ECTSI)

HSP 1 Score: 238 bits (608), Expect = 1.850e-78
Identity = 110/129 (85.27%), Postives = 121/129 (93.80%), Query Frame = 1 
Query:    1 QVRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            +VRFKTFDLGGHETARKLWKDYFTTVDGVVFL+DA D +RFPEAKKELDSLLTDE LQ VPFL+LGNKIDM  A SE++LRYAMGL+DT+GK +KPD+NPGVRPIELYMCSVVKRMGY+DGFKWLSQFI
Sbjct:   63 KVRFKTFDLGGHETARKLWKDYFTTVDGVVFLVDALDRQRFPEAKKELDSLLTDENLQTVPFLVLGNKIDMQAAVSEDELRYAMGLYDTFGKASKPDSNPGVRPIELYMCSVVKRMGYSDGFKWLSQFI 191
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: A0A7S2V1B9_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V1B9_9STRA)

HSP 1 Score: 221 bits (564), Expect = 9.570e-72
Identity = 105/130 (80.77%), Postives = 118/130 (90.77%), Query Frame = 1 
Query:    1 QVRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKP-DANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            +VRFKTFDLGGHETARKLW+DYFTTVDGVVFL+DA D ERFPEAKKELD+LLT E L NVPFL+LGNKID+A AASEE+LRYA+GLF+TYGK+ K  D +  VRPIELYMCSVV+RMGYADGFKWLSQF+
Sbjct:   63 KVRFKTFDLGGHETARKLWRDYFTTVDGVVFLVDALDRERFPEAKKELDALLTSEELANVPFLVLGNKIDIARAASEEELRYALGLFETYGKENKSADKDSNVRPIELYMCSVVRRMGYADGFKWLSQFL 192
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: A0A7S0C7R7_9STRA (Hypothetical protein n=1 Tax=Proboscia inermis TaxID=420281 RepID=A0A7S0C7R7_9STRA)

HSP 1 Score: 216 bits (550), Expect = 2.760e-70
Identity = 98/128 (76.56%), Postives = 116/128 (90.62%), Query Frame = 1 
Query:    4 VRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            ++FKTFDLGGHETAR+LW+DYFTTVDGVV+L+DA D  RFPEAKKELDSLL+ + L +VPFL+LGNKIDM  AASE++L+YA+GL DTYGK+TKPD + GVRPIELYMCSVV+RMGYADGFKWLS F+
Sbjct:   17 IKFKTFDLGGHETARRLWQDYFTTVDGVVYLVDAIDRGRFPEAKKELDSLLSSDELADVPFLVLGNKIDMPTAASEDELKYALGLMDTYGKETKPDKDSGVRPIELYMCSVVRRMGYADGFKWLSGFL 144
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: B7FXX2_PHATC (Predicted protein n=7 Tax=Bacillariophyta TaxID=2836 RepID=B7FXX2_PHATC)

HSP 1 Score: 217 bits (553), Expect = 4.520e-70
Identity = 98/128 (76.56%), Postives = 116/128 (90.62%), Query Frame = 1 
Query:    4 VRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            ++FKTFDLGGHETAR+LW+DYFTTVDGVV+L+DA D  RFPEAKKELD+LLT E LQ+VPFL+LGNKIDM  AASEE+L+YA+GL DTYGKD  PD++  VRPIELYMCSV++RMGY+DGFKWLSQF+
Sbjct:   64 IKFKTFDLGGHETARRLWQDYFTTVDGVVYLVDAIDRGRFPEAKKELDALLTSEELQDVPFLVLGNKIDMPTAASEEELKYALGLMDTYGKDKGPDSSSSVRPIELYMCSVIRRMGYSDGFKWLSQFL 191
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: B8BUA4_THAPS (Arf-type small G protein n=5 Tax=Bacillariophyta TaxID=2836 RepID=B8BUA4_THAPS)

HSP 1 Score: 216 bits (549), Expect = 1.840e-69
Identity = 97/129 (75.19%), Postives = 116/129 (89.92%), Query Frame = 1 
Query:    1 QVRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            Q++ KTFDLGGHETAR+LW+DYFTTVDGVV+L+DA D  RFPEAK+ELD+LLT + L +VPFL+LGNKIDM  AASEE+L+YA+GL DTYGKDTKPD N GVRPIE++MCSVV+RMGY DGF+WLSQF+
Sbjct:   63 QLKLKTFDLGGHETARRLWQDYFTTVDGVVYLVDAIDRGRFPEAKRELDALLTSDELVDVPFLVLGNKIDMPSAASEEELKYALGLLDTYGKDTKPDKNSGVRPIEVFMCSVVRRMGYKDGFQWLSQFL 191
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: A0A7S2LHQ1_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2LHQ1_9STRA)

HSP 1 Score: 214 bits (545), Expect = 1.470e-68
Identity = 101/130 (77.69%), Postives = 115/130 (88.46%), Query Frame = 1 
Query:    1 QVRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDA-NPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            +++FKTFDLGGHETAR+LW+DYFTTVDGVV+L+DA D  RFPEAKKELDSLLT + L  VPFL+LGNKIDM  AASEE+L+YA+GL DTYGKDTKP   N  VRPIELYMCSVV+RMGYADGFKWLSQF+
Sbjct:   83 KIKFKTFDLGGHETARRLWQDYFTTVDGVVYLVDAIDRGRFPEAKKELDSLLTSDELCGVPFLVLGNKIDMPTAASEEELKYALGLIDTYGKDTKPSQENSTVRPIELYMCSVVRRMGYADGFKWLSQFL 212
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: A0A7R9ZN47_9STRA (Hypothetical protein n=2 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9ZN47_9STRA)

HSP 1 Score: 212 bits (539), Expect = 2.140e-67
Identity = 97/128 (75.78%), Postives = 116/128 (90.62%), Query Frame = 1 
Query:    4 VRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            ++FKTFDLGGHETAR+LW+DYFTTVDGVV+L+DA D  RFPEAKKELD+LLT E LQ+VPFL+LGNKIDM  AASEE+L++A+GL DTYGKD  PD++ G RPIELYMCSV+++MGYADGFKWLSQF+
Sbjct:  104 IKFKTFDLGGHETARRLWQDYFTTVDGVVYLVDAIDRGRFPEAKKELDALLTSEELQDVPFLVLGNKIDMPTAASEEELKFALGLVDTYGKDKGPDSS-GTRPIELYMCSVIRKMGYADGFKWLSQFL 230
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: K8YVP7_NANGC (GTP-binding protein SAR1 n=3 Tax=Monodopsidaceae TaxID=425072 RepID=K8YVP7_NANGC)

HSP 1 Score: 209 bits (532), Expect = 9.800e-67
Identity = 96/130 (73.85%), Postives = 114/130 (87.69%), Query Frame = 1 
Query:    1 QVRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKP-DANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            ++RFKTFDLGGHETAR+LWKDY TTVDGVVF++DA D ERFPEAK+ELDSLLT + L +VPFL+LGNKID+  A SEE LRYA+G+ +TYGKD+   +  PGVRPIELYMCSV+KRMGYADGFKWL+QF+
Sbjct:   73 KIRFKTFDLGGHETARRLWKDYLTTVDGVVFIVDALDQERFPEAKRELDSLLTSDELAHVPFLVLGNKIDVPRAVSEEQLRYALGMQNTYGKDSSGGEKQPGVRPIELYMCSVIKRMGYADGFKWLAQFL 202
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: A0A1E7FLB6_9STRA (Small GTP-binding protein sar1 n=3 Tax=Bacillariaceae TaxID=33852 RepID=A0A1E7FLB6_9STRA)

HSP 1 Score: 206 bits (524), Expect = 1.210e-65
Identity = 95/128 (74.22%), Postives = 112/128 (87.50%), Query Frame = 1 
Query:    4 VRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            ++FKTFDLGGHETAR+LW DYFTTVDGVV+++D+ D  RFPEAKKELD+LLT E LQ+VPFL+LGNKIDM  AASEE+L++A+GL DTYGKD  PD   G RPIELYMCSV++RMGYADGFKWLS F+
Sbjct:   66 IKFKTFDLGGHETARRLWADYFTTVDGVVYIVDSIDRGRFPEAKKELDALLTTEELQDVPFLVLGNKIDMPTAASEEELKFALGLGDTYGKDKGPDGT-GTRPIELYMCSVIRRMGYADGFKWLSAFL 192
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BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Match: A0A812ZYF4_SYMMI (SAR1A protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812ZYF4_SYMMI)

HSP 1 Score: 204 bits (518), Expect = 9.560e-65
Identity = 93/130 (71.54%), Postives = 113/130 (86.92%), Query Frame = 1 
Query:    1 QVRFKTFDLGGHETARKLWKDYFTT-VDGVVFLIDAWDPERFPEAKKELDSLLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDANPGVRPIELYMCSVVKRMGYADGFKWLSQFI 387
            +++FKTFDLGGHE ARKLW++YF   VDGVVFL+DA D ERFPEAKKELD+LLT E L NVPFL+LGNKID+  AASE+DLR+ +GL++TYGK+TK + +  VRPIELYMCSVV++MGY DGFKWLSQF+
Sbjct:   63 KIKFKTFDLGGHEIARKLWREYFAAGVDGVVFLVDAVDRERFPEAKKELDALLTSEELANVPFLILGNKIDLGRAASEDDLRHQLGLYETYGKETKGERDANVRPIELYMCSVVRKMGYGDGFKWLSQFL 192
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig1784.3.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FXN4_ECTSI1.850e-7885.27Sar1A, Ras superfamily GTPase n=4 Tax=PX clade Tax... [more]
A0A7S2V1B9_9STRA9.570e-7280.77Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S0C7R7_9STRA2.760e-7076.56Hypothetical protein n=1 Tax=Proboscia inermis Tax... [more]
B7FXX2_PHATC4.520e-7076.56Predicted protein n=7 Tax=Bacillariophyta TaxID=28... [more]
B8BUA4_THAPS1.840e-6975.19Arf-type small G protein n=5 Tax=Bacillariophyta T... [more]
A0A7S2LHQ1_9STRA1.470e-6877.69Hypothetical protein n=1 Tax=Leptocylindrus danicu... [more]
A0A7R9ZN47_9STRA2.140e-6775.78Hypothetical protein n=2 Tax=Craspedostauros austr... [more]
K8YVP7_NANGC9.800e-6773.85GTP-binding protein SAR1 n=3 Tax=Monodopsidaceae T... [more]
A0A1E7FLB6_9STRA1.210e-6574.22Small GTP-binding protein sar1 n=3 Tax=Bacillariac... [more]
A0A812ZYF4_SYMMI9.560e-6571.54SAR1A protein n=1 Tax=Symbiodinium microadriaticum... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig1784contigP-wetherbeei_contig1784:5199..6524 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog2880.D7FXN4
PFAMsArf
Model size390
Max annot lvl2759|Eukaryota
KEGG koko:K07953
KEGG Pathwayko04141,ko05134,map04141,map05134
KEGG ModuleM00404
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005798,GO:0006810,GO:0006888,GO:0006900,GO:0006996,GO:0008150,GO:0009987,GO:0012505,GO:0012506,GO:0012507,GO:0016020,GO:0016043,GO:0016050,GO:0016192,GO:0022607,GO:0030117,GO:0030120,GO:0030127,GO:0030133,GO:0030134,GO:0030135,GO:0030658,GO:0030659,GO:0030660,GO:0030662,GO:0031090,GO:0031410,GO:0031982,GO:0032991,GO:0034622,GO:0035459,GO:0043226,GO:0043227,GO:0043229,GO:0043933,GO:0044085,GO:0044422,GO:0044424,GO:0044425,GO:0044433,GO:0044444,GO:0044446,GO:0044464,GO:0046907,GO:0048193,GO:0048194,GO:0048475,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0051668,GO:0061024,GO:0065003,GO:0071840,GO:0090110,GO:0090114,GO:0097708,GO:0098588,GO:0098796,GO:0098805
Exons4
Evalue3.04e-79
EggNOG OGsKOG0077@1|root,KOG0077@2759|Eukaryota
Ec32 ortholog descriptionSar1A, Ras superfamily GTPase
Ec32 orthologEc-28_002410.1
Descriptionintracellular protein transport
Cds size390
COG categoryU
BRITEko00000,ko00001,ko00002,ko01000,ko04031,ko04131
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186128.8280978-CDS-P-wetherbeei_contig1784:5198..52271683186128.8280978-CDS-P-wetherbeei_contig1784:5198..5227Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1784 5199..5227 -
1683186128.8378696-CDS-P-wetherbeei_contig1784:5597..56921683186128.8378696-CDS-P-wetherbeei_contig1784:5597..5692Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1784 5598..5692 -
1683186128.8455968-CDS-P-wetherbeei_contig1784:5996..61151683186128.8455968-CDS-P-wetherbeei_contig1784:5996..6115Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1784 5997..6115 -
1683186128.853244-CDS-P-wetherbeei_contig1784:6377..65241683186128.853244-CDS-P-wetherbeei_contig1784:6377..6524Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1784 6378..6524 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig1784.3.1prot_P-wetherbeei_contig1784.3.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig1784 5199..6524 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig1784.3.1

>prot_P-wetherbeei_contig1784.3.1 ID=prot_P-wetherbeei_contig1784.3.1|Name=mRNA_P-wetherbeei_contig1784.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=130bp
QVRFKTFDLGGHETARKLWKDYFTTVDGVVFLIDAWDPERFPEAKKELDS
LLTDETLQNVPFLLLGNKIDMAGAASEEDLRYAMGLFDTYGKDTKPDANP
GVRPIELYMCSVVKRMGYADGFKWLSQFI*
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mRNA from alignment at P-wetherbeei_contig1784:5199..6524-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig1784.3.1 ID=mRNA_P-wetherbeei_contig1784.3.1|Name=mRNA_P-wetherbeei_contig1784.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1326bp|location=Sequence derived from alignment at P-wetherbeei_contig1784:5199..6524- (Phaeothamnion wetherbeei SAG_119_79)
CAGGTGCGGTTCAAGACGTTCGATTTGGGGGGCCACGAAACGGCGCGTAA
GCTGTGGAAGGACTATTTCACTACCGTCGACGGCGTCGTCTTCCTCATCG
ACGCCTGGGATCCCGAGCGCTTCCCCGAGGCTAAAAAAGAGCTAGACGTG
AGTTGTACCTTTTCTGAAACGTCTCTTCTGAAACGTCTTTTTTTGTTTCT
GGCTTCTCCGGTCGAAAGATCCTGTCGCACTTCTCTCATCCGCATTGTTT
TCTCTTCTGTTTCTTCTTGCTGCATTTTTCCGTTGCTGCTCTCGCTGTCC
GGTGTATCACCTTTCCTTCTCTCTCCCAATTTCTGTTGTCCTTACTCTAC
TCTCGTTGCCTGCGATTCATCGTGACAACGCTACATCTGGCAACGGTAAA
TCAAAACAGTCATTGCTGACGGATGAGACACTGCAGAACGTGCCGTTCCT
GCTGCTCGGCAACAAGATCGACATGGCGGGGGCGGCCAGCGAGGAGGACC
TGCGCTACGCCATGGGGCTCTTCGACACGTGAGAAGAAGAGAAGAGGAGG
CCGTTTCTTTTAGCTGTTTCCCTGCCCCCGCCGTTCCGTTTCATCCCCGC
CGTTCTCCTGCCGTTCGGTGCCATTTCGCTGCCTTTGTGTCCGTCGCTGT
GTTGGGAAGGGGCGTTGCAGCTGGCGGTTTGGAATAAAGGCGATGTGCGT
CTACTGAGGCCGGACGATCTGACGTCAATGTGTTGGCAGCGAGCGCATCC
CGCTCTCATGATTGTGTTTCCCTTTCGCGCTCTCCATTTCTCGGCATTTA
ACTTTCGTTCTCTCCTTATCGGAATTTCACAGGTATGGCAAGGACACGAA
GCCCGACGCGAACCCGGGCGTGCGGCCCATCGAGCTCTACATGTGCTCCG
TCGTCAAGCGCATGGGCTACGCCGACGGTGAGACCGGATGACTGGTAGAA
AAGAGGAAAATACTCGCGGAGAAAGGGAGAACAAGGGATGGATGGAGGAA
GCAAGTGAGAAGGGTAGGTGCGGGCGGCTGCGAATGGCTGCAGTAGCCAG
CGGCTAAACGGAGGCAGAAAGATGGAGGTGGGAGGCGGAGGCGGCAGGCT
GGTGGCGGCAGGGAAGAGGATTGATTGCAGCATCTTTACTCATACCTGAT
CGGTCCCTACCTAGACCGGAGCGCCGGCAAGACGCGTGCGTTCAAGGTGG
TAGTGTTTTCAGGGCATGGATGTCTTCAGTACGAGCACGGAAAAAAGAGT
CTGACGCGGCATTTTCCGTCCCAACCCTTCCCACGCCCGGGCCGCAGGCT
TCAAGTGGCTTTCGCAATTCATATAA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig1784:5199..6524-

>mRNA_P-wetherbeei_contig1784.3.1 ID=mRNA_P-wetherbeei_contig1784.3.1|Name=mRNA_P-wetherbeei_contig1784.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=390bp|location=Sequence derived from alignment at P-wetherbeei_contig1784:5199..6524- (Phaeothamnion wetherbeei SAG_119_79)
CAGGTGCGGTTCAAGACGTTCGATTTGGGGGGCCACGAAACGGCGCGTAA
GCTGTGGAAGGACTATTTCACTACCGTCGACGGCGTCGTCTTCCTCATCG
ACGCCTGGGATCCCGAGCGCTTCCCCGAGGCTAAAAAAGAGCTAGACTCA
TTGCTGACGGATGAGACACTGCAGAACGTGCCGTTCCTGCTGCTCGGCAA
CAAGATCGACATGGCGGGGGCGGCCAGCGAGGAGGACCTGCGCTACGCCA
TGGGGCTCTTCGACACGTATGGCAAGGACACGAAGCCCGACGCGAACCCG
GGCGTGCGGCCCATCGAGCTCTACATGTGCTCCGTCGTCAAGCGCATGGG
CTACGCCGACGGCTTCAAGTGGCTTTCGCAATTCATATAA
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