mRNA_P-wetherbeei_contig9974.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig9974.2.1
Unique NamemRNA_P-wetherbeei_contig9974.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: L1JX35_GUITC (Sterol desaturase n=2 Tax=Guillardia theta TaxID=55529 RepID=L1JX35_GUITC)

HSP 1 Score: 155 bits (393), Expect = 3.660e-45
Identity = 67/110 (60.91%), Postives = 85/110 (77.27%), Query Frame = 1
Query:    7 MHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            MHLP  Y   HK HH YK+PQPFDDM+I+P+EA GYYCILYSPPFV  +H   FV YMA+MG CGILDH G++    G +Y+++DHD+HHE  + N+ FPF+WMD++HGT
Sbjct:  137 MHLPFFYKHFHKMHHSYKSPQPFDDMYIHPVEAVGYYCILYSPPFVFPLHVYGFVLYMAIMGVCGILDHSGIKWGFLG-IYNTEDHDKHHEHFDCNFAFPFVWMDILHGT 245          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: A0A7S1GXN0_HEMAN (Hypothetical protein (Fragment) n=2 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1GXN0_HEMAN)

HSP 1 Score: 147 bits (372), Expect = 1.610e-41
Identity = 70/110 (63.64%), Postives = 84/110 (76.36%), Query Frame = 1
Query:    7 MHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            MH    YSRLHK HH YK+P+PFDD++I+PLEA GYY ILYSPPF L +HA  F  YMA+MG CG+LDH GVR+R+PG LY ++DHD HH   EVNY FP   MD++HGT
Sbjct:  176 MHHRFFYSRLHKLHHFYKSPEPFDDLYIHPLEAAGYYAILYSPPFALPIHALGFGFYMAIMGLCGVLDHSGVRVRVPG-LYAAEDHDLHHSKFEVNYAFPHPLMDILHGT 284          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: A0A482SNF4_9ARCH (Fatty acid hydroxylase family protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SNF4_9ARCH)

HSP 1 Score: 145 bits (365), Expect = 6.370e-41
Identity = 62/110 (56.36%), Postives = 82/110 (74.55%), Query Frame = 1
Query:    7 MHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            MHL   Y   HK HH YKAP+PFDDM+I+P+EAFGYYCILY+PPF+  MH  SF+ YM +MG  G++DH G++  +P  +Y++ DHD HH   EVNY FPF ++D++HGT
Sbjct:  133 MHLKFFYIAFHKMHHQYKAPEPFDDMYIHPVEAFGYYCILYAPPFLFTMHYVSFIAYMVIMGIFGVMDHSGIKFSIPA-IYNTVDHDSHHAKFEVNYSFPFPYLDILHGT 241          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: A0A7S3D0N0_9EUKA (Hypothetical protein n=4 Tax=Palpitomonas bilix TaxID=652834 RepID=A0A7S3D0N0_9EUKA)

HSP 1 Score: 144 bits (363), Expect = 1.270e-40
Identity = 62/110 (56.36%), Postives = 82/110 (74.55%), Query Frame = 1
Query:    7 MHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            MHL   Y   HKYHH++K+P+PFDDM+I+PLEA GYY I+YS PF++ MH  +F GYM ++G  G++DH G++   P   Y S+DHD HHE  EVNY FPF+WMD++HGT
Sbjct:  137 MHLRWFYCTFHKYHHYFKSPEPFDDMYIHPLEALGYYIIMYSTPFLIPMHWTAFAGYMIVVGVAGVMDHSGIKFHFPL-FYASEDHDLHHEKYEVNYSFPFMWMDILHGT 245          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: C1FEX9_MICCC (C-4 sterol methyl oxidase n=1 Tax=Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) TaxID=296587 RepID=C1FEX9_MICCC)

HSP 1 Score: 143 bits (361), Expect = 3.450e-40
Identity = 65/113 (57.52%), Postives = 85/113 (75.22%), Query Frame = 1
Query:    1 QAMHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPF-VLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            +AMHLP +Y  LHK+HHHYK+PQP+DD+FI+P E+FGY  ILYSP F V  +   +F+ YM +MGACG+LDHCGV++R P G YD++ HD HH   + N+ FPF  MD +HGT
Sbjct:  163 RAMHLPRVYRLLHKFHHHYKSPQPWDDLFIHPAESFGYCLILYSPAFCVPSLPVQAFLLYMTIMGACGVLDHCGVKMRWPFGAYDTQFHDVHHRSFDANFAFPFPTMDRLHGT 275          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: A0A1V9YQH7_9STRA (Sterol desaturase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YQH7_9STRA)

HSP 1 Score: 142 bits (357), Expect = 5.580e-40
Identity = 64/110 (58.18%), Postives = 81/110 (73.64%), Query Frame = 1
Query:    7 MHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            +H  A+Y+R+HK HHHYK P+PFDDM+++P E   YY +LY PPFVL MH  SFV YM LMG  G+LDH GV +R+P  +YD++DHD HH     NYGFPF  +D+VHGT
Sbjct:  128 LHTRAMYTRIHKVHHHYKRPRPFDDMYMHPCEGMAYYWVLYGPPFVLPMHLHSFVVYMVLMGTFGLLDHSGVPVRIPF-VYDTRDHDVHHTRVTYNYGFPFQLLDVVHGT 236          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: A0A835YXK7_9STRA (Fatty acid hydroxylase domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YXK7_9STRA)

HSP 1 Score: 139 bits (350), Expect = 7.260e-40
Identity = 61/109 (55.96%), Postives = 75/109 (68.81%), Query Frame = 1
Query:   10 HLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            H P  Y R HKYHH+YK+P PFDD+FI+P+E F YYCIL++P   L +   +F  YM LMG CG+ DH GVR+ L  G YDS +HD HH    VN+GFPF W D +HGT
Sbjct:   52 HTPFFYRRFHKYHHYYKSPSPFDDLFIHPVEGFLYYCILFAPAAALPLRRAAFGAYMTLMGMCGLADHSGVRIAL--GPYDSAEHDLHHSHVNVNFGFPFAWFDRLHGT 158          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: W7TYR2_9STRA (C-4 sterol methyl oxidase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TYR2_9STRA)

HSP 1 Score: 141 bits (356), Expect = 3.230e-39
Identity = 65/110 (59.09%), Postives = 81/110 (73.64%), Query Frame = 1
Query:    7 MHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            MH   +Y R HK+HH YKAP PFDDM+I PLEA GYY ILYSP  VL MH  SF  Y+ L+G CGILDH G+ +RLP  +YD++ HD HH +T  N+GFPF ++D++HGT
Sbjct:  187 MHYGPVYRRFHKHHHFYKAPAPFDDMYIAPLEALGYYFILYSPALVLPMHVLSFAVYLTLLGLCGILDHSGIDVRLPR-VYDTRFHDIHHSYTNANFGFPFPFLDVLHGT 295          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: A0A5A8E491_CAFRO (Hypothetical protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8E491_CAFRO)

HSP 1 Score: 136 bits (343), Expect = 1.860e-37
Identity = 61/112 (54.46%), Postives = 78/112 (69.64%), Query Frame = 1
Query:    1 QAMHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            + MH P LY  +H  HH  K+P+PFDDM I+P+EA GYY ILYSP F+  MH   F+ YMA+MG  G+LDHCG  L LP  LYD++ HD HHE  +VN+GFP   +D++ GT
Sbjct:  154 RVMHFPWLYRNVHHLHHFNKSPEPFDDMLIHPVEAVGYYIILYSPAFLFPMHVSVFLAYMAIMGVTGVLDHCGAVLGLPLSLYDTRHHDHHHELVDVNFGFPSAAIDVLCGT 265          
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Match: A0A485KEW3_9STRA (Aste57867_4084 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KEW3_9STRA)

HSP 1 Score: 135 bits (340), Expect = 3.460e-37
Identity = 60/110 (54.55%), Postives = 81/110 (73.64%), Query Frame = 1
Query:    7 MHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMHAGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFPFLWMDMVHGT 336
            +H+ ALY R+HK HHHYK PQPFDDM+++P E   Y+CILY PPFVL M+  +F+ YM +MG  G+LDH G+   +P  +Y ++DH  HH    VNYGFPF+++D+VHGT
Sbjct:  130 LHIRALYVRVHKVHHHYKRPQPFDDMYMHPFEGAIYFCILYGPPFVLPMNVHAFILYMVVMGLFGVLDHSGLSFHIPL-VYKAQDHAVHHAKVNVNYGFPFVYLDLVHGT 238          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig9974.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
L1JX35_GUITC3.660e-4560.91Sterol desaturase n=2 Tax=Guillardia theta TaxID=5... [more]
A0A7S1GXN0_HEMAN1.610e-4163.64Hypothetical protein (Fragment) n=2 Tax=Hemiselmis... [more]
A0A482SNF4_9ARCH6.370e-4156.36Fatty acid hydroxylase family protein n=1 Tax=arch... [more]
A0A7S3D0N0_9EUKA1.270e-4056.36Hypothetical protein n=4 Tax=Palpitomonas bilix Ta... [more]
C1FEX9_MICCC3.450e-4057.52C-4 sterol methyl oxidase n=1 Tax=Micromonas commo... [more]
A0A1V9YQH7_9STRA5.580e-4058.18Sterol desaturase n=1 Tax=Achlya hypogyna TaxID=12... [more]
A0A835YXK7_9STRA7.260e-4055.96Fatty acid hydroxylase domain-containing protein n... [more]
W7TYR2_9STRA3.230e-3959.09C-4 sterol methyl oxidase n=1 Tax=Nannochloropsis ... [more]
A0A5A8E491_CAFRO1.860e-3754.46Hypothetical protein n=1 Tax=Cafeteria roenbergens... [more]
A0A485KEW3_9STRA3.460e-3754.55Aste57867_4084 protein n=1 Tax=Aphanomyces stellat... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig9974contigP-wetherbeei_contig9974:1912..2432 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog55529.EKX53146
PFAMsFA_hydroxylase
Model size336
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons2
Evalue1.96e-46
EggNOG OGsCOG3000@1|root,KOG0873@2759|Eukaryota
DescriptionC-4 methylsterol oxidase activity
Cds size330
COG categoryI
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683187119.3025913-CDS-P-wetherbeei_contig9974:1911..21471683187119.3025913-CDS-P-wetherbeei_contig9974:1911..2147Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig9974 1912..2147 -
1683187119.3165245-CDS-P-wetherbeei_contig9974:2332..24261683187119.3165245-CDS-P-wetherbeei_contig9974:2332..2426Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig9974 2333..2426 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683187119.3424754-UTR-P-wetherbeei_contig9974:2426..24321683187119.3424754-UTR-P-wetherbeei_contig9974:2426..2432Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig9974 2427..2432 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig9974.2.1prot_P-wetherbeei_contig9974.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig9974 1912..2426 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig9974.2.1

>prot_P-wetherbeei_contig9974.2.1 ID=prot_P-wetherbeei_contig9974.2.1|Name=mRNA_P-wetherbeei_contig9974.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=110bp
MHLPALYSRLHKYHHHYKAPQPFDDMFINPLEAFGYYCILYSPPFVLRMH
AGSFVGYMALMGACGILDHCGVRLRLPGGLYDSKDHDRHHEFTEVNYGFP
FLWMDMVHGT
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mRNA from alignment at P-wetherbeei_contig9974:1912..2432-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig9974.2.1 ID=mRNA_P-wetherbeei_contig9974.2.1|Name=mRNA_P-wetherbeei_contig9974.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=521bp|location=Sequence derived from alignment at P-wetherbeei_contig9974:1912..2432- (Phaeothamnion wetherbeei SAG_119_79)
CAGGCGATGCACCTACCGGCGCTTTACTCGCGCCTCCACAAGTACCACCA CCACTACAAGGCGCCGCAGCCGTTCGACGATATGTTTATTAACCCCCTCG GTAGGTCACGGCGGCCCAACTCGCGCTCTTCTTCGCTTTTTCAACGAAAC CTTCGCGGACTTTCCTGTCATTGATTTCCTCATGCAGCACAATATGATCC ATGGACCGGAAGGCTGTACGCAAAACAGCGCTCTCTTTCTCTTCTTTTTC ACCCCGCATCTCTCCACGCGGCACACTTCTCCCAGAGGCCTTCGGTTACT ACTGCATTCTCTACAGCCCACCGTTCGTGCTGAGGATGCATGCAGGCAGT TTCGTCGGTTACATGGCCCTCATGGGCGCCTGCGGCATCCTCGACCACTG CGGCGTCCGGCTGCGGCTCCCGGGAGGGCTGTACGACTCGAAGGACCACG ACCGGCACCACGAGTTCACAGAGGTGAATTACGGCTTTCCGTTCTTGTGG ATGGACATGGTGCACGGCACT
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Coding sequence (CDS) from alignment at P-wetherbeei_contig9974:1912..2432-

>mRNA_P-wetherbeei_contig9974.2.1 ID=mRNA_P-wetherbeei_contig9974.2.1|Name=mRNA_P-wetherbeei_contig9974.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=330bp|location=Sequence derived from alignment at P-wetherbeei_contig9974:1912..2432- (Phaeothamnion wetherbeei SAG_119_79)
ATGCACCTACCGGCGCTTTACTCGCGCCTCCACAAGTACCACCACCACTA
CAAGGCGCCGCAGCCGTTCGACGATATGTTTATTAACCCCCTCGAGGCCT
TCGGTTACTACTGCATTCTCTACAGCCCACCGTTCGTGCTGAGGATGCAT
GCAGGCAGTTTCGTCGGTTACATGGCCCTCATGGGCGCCTGCGGCATCCT
CGACCACTGCGGCGTCCGGCTGCGGCTCCCGGGAGGGCTGTACGACTCGA
AGGACCACGACCGGCACCACGAGTTCACAGAGGTGAATTACGGCTTTCCG
TTCTTGTGGATGGACATGGTGCACGGCACT
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