mRNA_P-wetherbeei_contig6831.3.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig6831.3.1
Unique NamemRNA_P-wetherbeei_contig6831.3.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: A0A3C0TVM3_9PROT (NADPH-dependent 7-cyano-7-deazaguanine reductase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A3C0TVM3_9PROT)

HSP 1 Score: 270 bits (690), Expect = 4.050e-91
Identity = 122/151 (80.79%), Postives = 134/151 (88.74%), Query Frame = 1 
Query:    1 MPKKPALKILGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGR 453
            M KKP L  LG+K + P SP+QA LE+  NPHPDTDYVARFTCPEFTSLCP+T QPDFA LMIDY+PGKWIVESKSLKLFLQS+RN+GAFHEDCTIAI KRIK+ IAPKWLRIGGYWYPRGG+PIDVFWQTG AP+ +WIPPQDVP YRGR
Sbjct:    1 MSKKPTLTQLGRKTAVPASPEQANLERAANPHPDTDYVARFTCPEFTSLCPITSQPDFAFLMIDYIPGKWIVESKSLKLFLQSFRNHGAFHEDCTIAIGKRIKSVIAPKWLRIGGYWYPRGGIPIDVFWQTGRAPEGVWIPPQDVPAYRGR 151
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: UPI001421FB0F (preQ(1) synthase n=1 Tax=Parvibaculum indicum TaxID=562969 RepID=UPI001421FB0F)

HSP 1 Score: 248 bits (633), Expect = 2.190e-82
Identity = 108/149 (72.48%), Postives = 128/149 (85.91%), Query Frame = 1 
Query:   10 KPALKILGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            KP +  LG+ V+ P SPD+A LE+V NPHPDTDY+ARFT PEFTS+CP+TGQPDFAHL+IDY PGKW+VESKSLKL+LQS+RN+GAFHEDCT+AI KR+ T + PKWLRIGGYWYPRGG+PIDVFWQTG  P+ +W+P Q V  YRGRG
Sbjct:    7 KPEVSQLGQSVAVPASPDEAELERVPNPHPDTDYIARFTAPEFTSICPVTGQPDFAHLVIDYAPGKWLVESKSLKLYLQSFRNHGAFHEDCTVAIGKRLATLLEPKWLRIGGYWYPRGGIPIDVFWQTGKLPEGVWVPDQGVQPYRGRG 155
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: UPI0015E7574F (preQ(1) synthase n=1 Tax=Hyphomicrobium methylovorum TaxID=84 RepID=UPI0015E7574F)

HSP 1 Score: 246 bits (627), Expect = 1.680e-81
Identity = 111/150 (74.00%), Postives = 128/150 (85.33%), Query Frame = 1 
Query:    7 KKPALKILGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            K P++K LGK V  P SPD+A LE+V NPH DT Y ARFT PEFTSLCP+TGQPDFAHL+IDYVPGKW+VESKSLKLFL S+RN+GAFHEDCT+AI KRI   I P++LRIGGYWYPRGG+PIDVFWQTG+ PK++W+P Q VP YRGRG
Sbjct:    4 KSPSVKQLGKSVGLPASPDEAELERVPNPHSDTHYAARFTAPEFTSLCPVTGQPDFAHLVIDYVPGKWLVESKSLKLFLASFRNHGAFHEDCTVAIGKRIADLIGPRYLRIGGYWYPRGGIPIDVFWQTGSLPKDVWLPDQGVPTYRGRG 153
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: A0A6L3EJ30_9PROT (NADPH-dependent 7-cyano-7-deazaguanine reductase n=1 Tax=Proteobacteria bacterium TaxID=1977087 RepID=A0A6L3EJ30_9PROT)

HSP 1 Score: 242 bits (618), Expect = 4.080e-80
Identity = 109/151 (72.19%), Postives = 127/151 (84.11%), Query Frame = 1 
Query:    4 PKKPALKILGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            PK  AL+ LGK V+ P  PDQA+LE+VVNP    DY+ RFTCPEFTSLCP+T QPDFAHL+IDYVPG+W+VESKSLKL+ QS+RN+GAFHEDCT+ IA+ I   + PKWLRIGGYWYPRGGMPIDVF+QTG+ P +LWIP Q VPGYRGRG
Sbjct:    4 PKSDALEQLGKPVAVPDHPDQALLERVVNPRQKADYLVRFTCPEFTSLCPVTSQPDFAHLVIDYVPGRWLVESKSLKLYFQSFRNHGAFHEDCTVQIAETIVDLLKPKWLRIGGYWYPRGGMPIDVFYQTGSPPADLWIPDQGVPGYRGRG 154
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: A0A7W6D3D0_9HYPH (NADPH-dependent 7-cyano-7-deazaguanine reductase n=1 Tax=Hansschlegelia beijingensis TaxID=1133344 RepID=A0A7W6D3D0_9HYPH)

HSP 1 Score: 241 bits (616), Expect = 6.730e-80
Identity = 107/143 (74.83%), Postives = 122/143 (85.31%), Query Frame = 1 
Query:   28 LGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            LGK+   P SPD+A L++V NPH DTDYV RF  PEFTSLCP+TGQPDFAHL+IDYVPG W+VESK+LKLFL ++RN+GAFHEDCT+AI KR+  AIAP+WLRIGGYWYPRGG+PIDVFWQTG AP  LWIP Q VP YRGRG
Sbjct:    6 LGKQTPLPTSPDEAQLDRVPNPHADTDYVTRFVAPEFTSLCPVTGQPDFAHLVIDYVPGDWLVESKALKLFLGAFRNHGAFHEDCTVAIGKRLVAAIAPRWLRIGGYWYPRGGIPIDVFWQTGPAPAGLWIPDQGVPPYRGRG 148
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: X5MN66_9HYPH (NADPH-dependent 7-cyano-7-deazaguanine reductase n=1 Tax=Candidatus Phaeomarinobacter ectocarpi TaxID=1458461 RepID=X5MN66_9HYPH)

HSP 1 Score: 241 bits (615), Expect = 1.090e-79
Identity = 105/143 (73.43%), Postives = 125/143 (87.41%), Query Frame = 1 
Query:   28 LGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            LG++ + P SP++A LE+V NPHP TDYVARFT PEFTSLCP+TGQPDFAH++IDYVPG+W+VESKSLKL+L S+RN+GAFHEDCTIAIAKR+   + PKWLRIGGYWYPRGG+PIDVFWQTG  P+ +W+P Q VP YRGRG
Sbjct:   10 LGQQTALPASPEEADLERVANPHPGTDYVARFTAPEFTSLCPVTGQPDFAHIVIDYVPGEWLVESKSLKLYLGSFRNHGAFHEDCTIAIAKRLADLLKPKWLRIGGYWYPRGGIPIDVFWQTGDVPQGVWLPDQGVPAYRGRG 152
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: F8JCK4_HYPSM (NADPH-dependent 7-cyano-7-deazaguanine reductase n=1 Tax=Hyphomicrobium sp. (strain MC1) TaxID=717785 RepID=F8JCK4_HYPSM)

HSP 1 Score: 241 bits (614), Expect = 1.610e-79
Identity = 108/149 (72.48%), Postives = 128/149 (85.91%), Query Frame = 1 
Query:   10 KPALKILGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            KP +  LGK+V  P SP++A LE+V NPH DTDY+ARFT PEFTSLCP+TGQPDFAHL+IDYVPGKW++ESKSLKL+L ++RN+GAFHEDCT+AI KRI   I PK+LRIGGYWYPRGG+PIDVFWQTGAAP ++W+P Q V  YRGRG
Sbjct:    5 KPDVTQLGKQVGIPDSPEKAELERVANPHTDTDYLARFTAPEFTSLCPVTGQPDFAHLVIDYVPGKWLLESKSLKLYLAAFRNHGAFHEDCTVAIGKRIAALIKPKYLRIGGYWYPRGGIPIDVFWQTGAAPADIWLPDQGVAPYRGRG 153
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: A0A433A7S8_HYPSQ (NADPH-dependent 7-cyano-7-deazaguanine reductase n=1 Tax=Hyphomicrobium sp. TaxID=82 RepID=A0A433A7S8_HYPSQ)

HSP 1 Score: 240 bits (613), Expect = 2.280e-79
Identity = 108/149 (72.48%), Postives = 127/149 (85.23%), Query Frame = 1 
Query:   10 KPALKILGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            KPA+  LGK+V  P+SP++A LE+V NPH DTDYVARFT PEFTSLCP+TGQPDFAH +IDYVPGKW++ESKSLKL+L S+RN+GAFHEDCT+ I KRI   I PK+LRIGGYWYPRGG+PIDVFWQTG  PK++W+P Q V  YRGRG
Sbjct:    5 KPAVSQLGKQVGIPESPEKAELERVPNPHADTDYVARFTAPEFTSLCPVTGQPDFAHFVIDYVPGKWLLESKSLKLYLASFRNHGAFHEDCTVVIGKRIAGLIKPKYLRIGGYWYPRGGIPIDVFWQTGTLPKDVWLPDQGVQTYRGRG 153
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: A0A3B0RW72_9ZZZZ (NADPH-dependent 7-cyano-7-deazaguanine reductase n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B0RW72_9ZZZZ)

HSP 1 Score: 240 bits (613), Expect = 2.280e-79
Identity = 110/151 (72.85%), Postives = 124/151 (82.12%), Query Frame = 1 
Query:    4 PKKPALKILGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            P KP L  LG     P SP+QA+LE+V NPHP  DY+ RF  PEFTSLCP+TGQPDFAHL+IDYVP KW+VESKSLKL+L S+RN+GAFHEDCTIAI KRI   +APKWLRIGGYWYPRGG+PIDVFWQTG  P  +W+P QDVPGYRGRG
Sbjct:    4 PTKP-LTQLGGTSKLPASPEQAVLERVANPHPGDDYLVRFAAPEFTSLCPVTGQPDFAHLVIDYVPAKWLVESKSLKLYLGSFRNHGAFHEDCTIAIGKRIVETLAPKWLRIGGYWYPRGGIPIDVFWQTGEIPNEVWVPEQDVPGYRGRG 153
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BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Match: A0A2D4VFU5_9HYPH (NADPH-dependent 7-cyano-7-deazaguanine reductase n=1 Tax=Parvibaculum sp. TaxID=2024848 RepID=A0A2D4VFU5_9HYPH)

HSP 1 Score: 240 bits (613), Expect = 2.440e-79
Identity = 104/143 (72.73%), Postives = 122/143 (85.31%), Query Frame = 1 
Query:   28 LGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLCPMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAKRIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRGRG 456
            LG+ V+   SP+ A LE+V NPHPDTDY+ARFT PEFTSLCP+TGQPDFAHL+IDY PGKW+VESKSLKL+ QS+RN+GAFHEDCT+AI KR+ T + PKWLRIGGYWYPRGG+PIDVFWQTG  P+ +W+P Q V  YRGRG
Sbjct:   13 LGQNVAPAASPEDAQLERVPNPHPDTDYIARFTAPEFTSLCPVTGQPDFAHLVIDYAPGKWLVESKSLKLYFQSFRNHGAFHEDCTVAIGKRLNTLLKPKWLRIGGYWYPRGGIPIDVFWQTGKLPEGVWVPDQGVQPYRGRG 155
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig6831.3.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3C0TVM3_9PROT4.050e-9180.79NADPH-dependent 7-cyano-7-deazaguanine reductase n... [more]
UPI001421FB0F2.190e-8272.48preQ(1) synthase n=1 Tax=Parvibaculum indicum TaxI... [more]
UPI0015E7574F1.680e-8174.00preQ(1) synthase n=1 Tax=Hyphomicrobium methylovor... [more]
A0A6L3EJ30_9PROT4.080e-8072.19NADPH-dependent 7-cyano-7-deazaguanine reductase n... [more]
A0A7W6D3D0_9HYPH6.730e-8074.83NADPH-dependent 7-cyano-7-deazaguanine reductase n... [more]
X5MN66_9HYPH1.090e-7973.43NADPH-dependent 7-cyano-7-deazaguanine reductase n... [more]
F8JCK4_HYPSM1.610e-7972.48NADPH-dependent 7-cyano-7-deazaguanine reductase n... [more]
A0A433A7S8_HYPSQ2.280e-7972.48NADPH-dependent 7-cyano-7-deazaguanine reductase n... [more]
A0A3B0RW72_9ZZZZ2.280e-7972.85NADPH-dependent 7-cyano-7-deazaguanine reductase n... [more]
A0A2D4VFU5_9HYPH2.440e-7972.73NADPH-dependent 7-cyano-7-deazaguanine reductase n... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig6831contigP-wetherbeei_contig6831:2017..2475 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog670307.HYPDE_30688
Preferred namequeF
PFAMsQueF
Model size459
Max annot lvl28211|Alphaproteobacteria
KEGG rclassRC01875
KEGG koko:K09457
KEGG ReactionR07605
KEGG Pathwayko00790,ko01100,map00790,map01100
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.79e-80
EggNOG OGsCOG0780@1|root,COG0780@2|Bacteria,1MW0M@1224|Proteobacteria,2U57X@28211|Alphaproteobacteria,3N6RD@45401|Hyphomicrobiaceae
EC1.7.1.13
DescriptionCatalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)
Cds size459
COG categoryH
BRITEko00000,ko00001,ko01000,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186940.5589936-CDS-P-wetherbeei_contig6831:2016..24751683186940.5589936-CDS-P-wetherbeei_contig6831:2016..2475Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig6831 2017..2475 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig6831.3.1prot_P-wetherbeei_contig6831.3.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig6831 2017..2475 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig6831.3.1

>prot_P-wetherbeei_contig6831.3.1 ID=prot_P-wetherbeei_contig6831.3.1|Name=mRNA_P-wetherbeei_contig6831.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=153bp
MPKKPALKILGKKVSAPKSPDQAMLEKVVNPHPDTDYVARFTCPEFTSLC
PMTGQPDFAHLMIDYVPGKWIVESKSLKLFLQSYRNYGAFHEDCTIAIAK
RIKTAIAPKWLRIGGYWYPRGGMPIDVFWQTGAAPKNLWIPPQDVPGYRG
RG*
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mRNA from alignment at P-wetherbeei_contig6831:2017..2475-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig6831.3.1 ID=mRNA_P-wetherbeei_contig6831.3.1|Name=mRNA_P-wetherbeei_contig6831.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=459bp|location=Sequence derived from alignment at P-wetherbeei_contig6831:2017..2475- (Phaeothamnion wetherbeei SAG_119_79)
ATGCCCAAAAAACCAGCCCTTAAAATTCTCGGGAAAAAAGTTTCCGCACC
GAAATCACCGGATCAGGCGATGCTGGAGAAAGTAGTGAATCCGCATCCCG
ATACGGATTATGTGGCGCGCTTTACCTGCCCGGAATTTACGTCATTGTGT
CCCATGACCGGCCAGCCGGATTTTGCGCATCTGATGATTGATTATGTGCC
GGGCAAGTGGATTGTTGAATCCAAATCGCTGAAACTGTTTTTACAATCCT
ACCGCAATTACGGGGCGTTTCATGAGGATTGCACCATCGCCATCGCCAAG
CGCATCAAGACGGCGATAGCCCCCAAATGGCTGCGCATCGGGGGCTATTG
GTATCCGCGCGGCGGCATGCCGATTGATGTGTTCTGGCAAACCGGTGCGG
CGCCCAAAAATCTGTGGATCCCGCCGCAGGATGTTCCCGGCTATCGCGGG
CGCGGCTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig6831:2017..2475-

>mRNA_P-wetherbeei_contig6831.3.1 ID=mRNA_P-wetherbeei_contig6831.3.1|Name=mRNA_P-wetherbeei_contig6831.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=459bp|location=Sequence derived from alignment at P-wetherbeei_contig6831:2017..2475- (Phaeothamnion wetherbeei SAG_119_79)
ATGCCCAAAAAACCAGCCCTTAAAATTCTCGGGAAAAAAGTTTCCGCACC
GAAATCACCGGATCAGGCGATGCTGGAGAAAGTAGTGAATCCGCATCCCG
ATACGGATTATGTGGCGCGCTTTACCTGCCCGGAATTTACGTCATTGTGT
CCCATGACCGGCCAGCCGGATTTTGCGCATCTGATGATTGATTATGTGCC
GGGCAAGTGGATTGTTGAATCCAAATCGCTGAAACTGTTTTTACAATCCT
ACCGCAATTACGGGGCGTTTCATGAGGATTGCACCATCGCCATCGCCAAG
CGCATCAAGACGGCGATAGCCCCCAAATGGCTGCGCATCGGGGGCTATTG
GTATCCGCGCGGCGGCATGCCGATTGATGTGTTCTGGCAAACCGGTGCGG
CGCCCAAAAATCTGTGGATCCCGCCGCAGGATGTTCCCGGCTATCGCGGG
CGCGGCTGA
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