mRNA_P-wetherbeei_contig55828.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig55828.1.1
Unique NamemRNA_P-wetherbeei_contig55828.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: A0A090MHG6_AFIFE (Respiratory nitrate reductase 1 beta chain n=2 Tax=Afipia felis TaxID=1035 RepID=A0A090MHG6_AFIFE)

HSP 1 Score: 327 bits (838), Expect = 7.310e-110
Identity = 158/173 (91.33%), Postives = 168/173 (97.11%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAGQPTVCSETCVGRIRYLGVVLYDADRI +AASKPDERDLYQAQLDVFL+PNDP+VIAEAE QGIPHAWLEAA  SPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDG+MPDV+SLRIPM+YLANLLTAG+E+PV LALERM+AMRSYMRAKT+D
Sbjct:  130 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIAEAASKPDERDLYQAQLDVFLDPNDPSVIAEAERQGIPHAWLEAAKASPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGEMPDVRSLRIPMRYLANLLTAGNEEPVALALERMLAMRSYMRAKTVD 302
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: B6JBW3_AFIC5 (Respiratory nitrate reductase beta chain NarH n=25 Tax=Bradyrhizobiaceae TaxID=41294 RepID=B6JBW3_AFIC5)

HSP 1 Score: 325 bits (833), Expect = 1.620e-107
Identity = 157/173 (90.75%), Postives = 168/173 (97.11%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAGQPTVCSETCVGRIRYLGVVLYDADRI +AASKPDERDLYQAQLDVFLNP+DPAVIAEAE QGIP+AWLEAA KSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDG+MPDV+SLRIP++YLANLLTAGDE+PV  ALERM+AMR+YMRAKT+D
Sbjct:  249 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIAEAASKPDERDLYQAQLDVFLNPHDPAVIAEAERQGIPYAWLEAAKKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGEMPDVRSLRIPVRYLANLLTAGDEEPVAQALERMLAMRAYMRAKTVD 421
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: A0A4R9ZI01_9BACT (Nitrate reductase subunit beta n=52 Tax=Bacteria TaxID=2 RepID=A0A4R9ZI01_9BACT)

HSP 1 Score: 305 bits (781), Expect = 1.200e-99
Identity = 146/173 (84.39%), Postives = 160/173 (92.49%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAGQPTVCSETCVGRIRYLGV+LYDADRI  AAS PDE+DLYQAQLDVFLNPNDPAV+A+A   GIP  WLEAA  SP+WKMAMEWK+AFPLHPEYRTLPMVWYVPPLSPI SAA+AGK+G+DGDMPDV+SLRIP++YLANLLTAG E+PV LALERM+AMRSYMRAKTID
Sbjct:  249 RIEAGQPTVCSETCVGRIRYLGVILYDADRIEAAASTPDEKDLYQAQLDVFLNPNDPAVLAQARADGIPDNWLEAAKLSPVWKMAMEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGKMGVDGDMPDVRSLRIPLQYLANLLTAGKEEPVALALERMLAMRSYMRAKTID 421
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: A0A833LGJ2_9HYPH (Nitrate reductase subunit beta n=1 Tax=Bauldia sp. TaxID=2575872 RepID=A0A833LGJ2_9HYPH)

HSP 1 Score: 301 bits (771), Expect = 2.280e-99
Identity = 145/173 (83.82%), Postives = 158/173 (91.33%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAGQPTVCSETCVGRIRYLGV+LYDADRI  AA   D++DLYQAQLDVFL+PNDPAVIAEA  QGIP  W+E A +SPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPI SAA+AG IGLDGDMPDV+SLRIP+KYLANLLTAG E+PV LALERM+AMR+YMRAKT+D
Sbjct:  155 RIEAGQPTVCSETCVGRIRYLGVILYDADRIEAAAGVADDQDLYQAQLDVFLDPNDPAVIAEARAQGIPENWIEGAVRSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGDIGLDGDMPDVRSLRIPLKYLANLLTAGKEEPVALALERMLAMRAYMRAKTVD 327
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: A0A5C1W8Y1_9HYPH (Nitrate reductase subunit beta n=3 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A5C1W8Y1_9HYPH)

HSP 1 Score: 302 bits (774), Expect = 1.360e-98
Identity = 143/173 (82.66%), Postives = 161/173 (93.06%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAGQPTVCSETCVGRIRYLGVVLYDADRI  AA+ PDE+DLYQAQLD+FL+P DPAVIA+A + G+P AW+EAA  SPIWKMAMEWK+AFPLHPEYRTLPMVWYVPPLSPI SAA+AG++GLDGDMPDV+SLRIP++YLANLLTAG E+PV LALERM+AMRSYMRAKT+D
Sbjct:  249 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIEAAAATPDEKDLYQAQLDLFLDPKDPAVIAQARVDGVPDAWIEAARHSPIWKMAMEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGRMGLDGDMPDVRSLRIPLRYLANLLTAGKEEPVALALERMLAMRSYMRAKTVD 421
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: A0A554U1A6_9MYCO (Nitrate reductase subunit beta n=3 Tax=Bacteria TaxID=2 RepID=A0A554U1A6_9MYCO)

HSP 1 Score: 301 bits (770), Expect = 5.450e-98
Identity = 142/173 (82.08%), Postives = 160/173 (92.49%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAGQPTVCSETCVGRIRYLGVVLYDADRI  AA  PDE+DLY+AQLD+FL+PN+PAVIA+A   G+P AWLEAA  SP+WKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPI SAA+AG+IG+DG+MPDV+SLRIP++YLANLLTAGDE+PV LALERM+AMR YMRAKT+D
Sbjct:  249 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIEAAAGTPDEKDLYKAQLDLFLDPNNPAVIAQARADGVPEAWLEAAKHSPVWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGRIGVDGEMPDVRSLRIPLRYLANLLTAGDEEPVALALERMLAMRGYMRAKTVD 421
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: A0A1G6C9I2_9HYPH (Respiratory nitrate reductase beta subunit n=1 Tax=Bauldia litoralis TaxID=665467 RepID=A0A1G6C9I2_9HYPH)

HSP 1 Score: 297 bits (760), Expect = 1.650e-96
Identity = 143/173 (82.66%), Postives = 157/173 (90.75%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAGQPTVCSETCVGRIRYLGV+LYDADRI  AAS  DE+DLYQAQLDVFL+PNDP VIAEA  QGIP  W+E A +SPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPI SAA+AG IG+DG+MPDV+SLRIP+KYLANLLTAG E+PV LALERM+AMR YMRA+T+D
Sbjct:  249 RIEAGQPTVCSETCVGRIRYLGVILYDADRIEAAASVADEQDLYQAQLDVFLDPNDPEVIAEARAQGIPGNWIEGAIRSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGSIGVDGEMPDVRSLRIPLKYLANLLTAGKEEPVALALERMLAMRGYMRARTVD 421
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: UPI0003F9AB24 (nitrate reductase subunit beta n=2 Tax=Salinarimonas TaxID=690086 RepID=UPI0003F9AB24)

HSP 1 Score: 296 bits (758), Expect = 3.600e-96
Identity = 141/173 (81.50%), Postives = 158/173 (91.33%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            R+EAG PTVCSETCVGRIRYLGVVLYDADRI +AAS PDE DLY+AQL VFL+PNDPAVIAEA  +GIP AWLEAA +SP+WKMAMEWK+AFPLHPEYRTLPMVWYVPPLSPI SAA AGKIG+DG+MPDVK+LRIP++YLANLLTAG E+PV   LERM+AMR++MRAKTID
Sbjct:  249 RLEAGMPTVCSETCVGRIRYLGVVLYDADRIQEAASTPDEGDLYEAQLKVFLDPNDPAVIAEARAEGIPDAWLEAARRSPVWKMAMEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAAEAGKIGVDGEMPDVKNLRIPVRYLANLLTAGREEPVVTGLERMLAMRAFMRAKTID 421
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: A0A387G2P1_9HYPH (Nitrate reductase subunit beta n=7 Tax=Hyphomicrobiales TaxID=356 RepID=A0A387G2P1_9HYPH)

HSP 1 Score: 296 bits (757), Expect = 4.950e-96
Identity = 141/173 (81.50%), Postives = 158/173 (91.33%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAGQPTVCSETCVGRIRYLGV+LYDADRI  AAS PDE+DLYQAQLD+FL+P DP VIA+A   G+P AW+EAA  SPIWKMAM+WKVAFPLHPEYRTLPMVWYVPPLSPI SAA+AG++GLDGDMPDV+SLRIP++YLANLLTAG+E PV LALERM+AMR YMRAK+ID
Sbjct:  249 RIEAGQPTVCSETCVGRIRYLGVLLYDADRIEAAASVPDEKDLYQAQLDLFLDPKDPKVIAQARADGVPEAWIEAALTSPIWKMAMDWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGRMGLDGDMPDVRSLRIPLQYLANLLTAGNEDPVALALERMLAMRGYMRAKSID 421
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BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Match: A0A6N9AYH5_9GAMM (Nitrate reductase subunit beta (Fragment) n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A6N9AYH5_9GAMM)

HSP 1 Score: 288 bits (737), Expect = 5.170e-96
Identity = 137/173 (79.19%), Postives = 155/173 (89.60%), Query Frame = 1 
Query:    1 RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDVFLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDEKPVTLALERMMAMRSYMRAKTID 519
            RIEAG+PTVCSETCVGRIRYLGVVLYDADRI +AAS  DE DLY+AQL +FL+PNDP VIA+A    +P AWLEAAT+SP+WK+AMEWKVAFPLHPEYRTLPMVWYVPPLSPI SAA AGKIG+D +MPDV+SLRIP++YLANLLTAG E+PV  ALERM+AMR YMRAKT+D
Sbjct:   22 RIEAGEPTVCSETCVGRIRYLGVVLYDADRIREAASVEDEHDLYEAQLGIFLDPNDPDVIAQARADMVPDAWLEAATRSPVWKLAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAEAGKIGIDNEMPDVRSLRIPIRYLANLLTAGKEEPVARALERMLAMRGYMRAKTVD 194
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig55828.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A090MHG6_AFIFE7.310e-11091.33Respiratory nitrate reductase 1 beta chain n=2 Tax... [more]
B6JBW3_AFIC51.620e-10790.75Respiratory nitrate reductase beta chain NarH n=25... [more]
A0A4R9ZI01_9BACT1.200e-9984.39Nitrate reductase subunit beta n=52 Tax=Bacteria T... [more]
A0A833LGJ2_9HYPH2.280e-9983.82Nitrate reductase subunit beta n=1 Tax=Bauldia sp.... [more]
A0A5C1W8Y1_9HYPH1.360e-9882.66Nitrate reductase subunit beta n=3 Tax=Alphaproteo... [more]
A0A554U1A6_9MYCO5.450e-9882.08Nitrate reductase subunit beta n=3 Tax=Bacteria Ta... [more]
A0A1G6C9I2_9HYPH1.650e-9682.66Respiratory nitrate reductase beta subunit n=1 Tax... [more]
UPI0003F9AB243.600e-9681.50nitrate reductase subunit beta n=2 Tax=Salinarimon... [more]
A0A387G2P1_9HYPH4.950e-9681.50Nitrate reductase subunit beta n=7 Tax=Hyphomicrob... [more]
A0A6N9AYH5_9GAMM5.170e-9679.19Nitrate reductase subunit beta (Fragment) n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig55828contigP-wetherbeei_contig55828:1..519 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1304878.AUGD01000033_gene3253
Preferred namenarH
PFAMsFer4_11,Nitr_red_bet_C
Model size519
Max annot lvl28211|Alphaproteobacteria
KEGG rclassRC02812
KEGG koko:K00371
KEGG TC5.A.3.1
KEGG ReactionR00798,R01106,R09497
KEGG Pathwayko00910,ko01120,ko02020,map00910,map01120,map02020
KEGG ModuleM00529,M00530,M00804
Hectar predicted targeting categoryother localisation
Exons1
Evalue4.09e-110
EggNOG OGsCOG1140@1|root,COG1140@2|Bacteria,1MW9Q@1224|Proteobacteria,2VDR0@28211|Alphaproteobacteria,3JWZN@41294|Bradyrhizobiaceae
EC1.7.5.1
DescriptionRespiratory nitrate reductase beta C-terminal
Cds size519
COG categoryC
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186830.9008985-CDS-P-wetherbeei_contig55828:0..5191683186830.9008985-CDS-P-wetherbeei_contig55828:0..519Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig55828 1..519 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig55828.1.1prot_P-wetherbeei_contig55828.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig55828 1..519 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig55828.1.1

>prot_P-wetherbeei_contig55828.1.1 ID=prot_P-wetherbeei_contig55828.1.1|Name=mRNA_P-wetherbeei_contig55828.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=173bp
RIEAGQPTVCSETCVGRIRYLGVVLYDADRIVDAASKPDERDLYQAQLDV
FLNPNDPAVIAEAEMQGIPHAWLEAATKSPIWKMAMEWKVAFPLHPEYRT
LPMVWYVPPLSPITSAASAGKIGLDGDMPDVKSLRIPMKYLANLLTAGDE
KPVTLALERMMAMRSYMRAKTID
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mRNA from alignment at P-wetherbeei_contig55828:1..519-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig55828.1.1 ID=mRNA_P-wetherbeei_contig55828.1.1|Name=mRNA_P-wetherbeei_contig55828.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=519bp|location=Sequence derived from alignment at P-wetherbeei_contig55828:1..519- (Phaeothamnion wetherbeei SAG_119_79)
CGCATCGAGGCCGGCCAGCCGACGGTGTGCTCGGAGACCTGCGTCGGGCG
CATCCGCTACCTCGGCGTGGTTCTCTACGACGCCGATAGAATCGTCGACG
CCGCGAGCAAGCCGGATGAGCGCGATCTCTATCAGGCTCAGCTCGACGTG
TTCCTCAACCCGAACGACCCTGCTGTCATAGCGGAGGCGGAAATGCAGGG
CATTCCGCATGCGTGGCTCGAAGCTGCCACAAAATCGCCGATCTGGAAGA
TGGCGATGGAATGGAAGGTCGCGTTCCCGCTGCATCCGGAATATCGCACC
TTGCCGATGGTCTGGTATGTGCCGCCACTGTCGCCGATCACATCGGCGGC
GTCCGCCGGCAAGATCGGCCTCGACGGCGACATGCCCGACGTCAAATCGC
TGCGCATTCCGATGAAGTATCTCGCCAACCTGCTGACCGCCGGCGACGAG
AAGCCCGTTACGCTTGCGCTGGAGCGAATGATGGCGATGCGGTCCTACAT
GCGGGCGAAGACCATCGAC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig55828:1..519-

>mRNA_P-wetherbeei_contig55828.1.1 ID=mRNA_P-wetherbeei_contig55828.1.1|Name=mRNA_P-wetherbeei_contig55828.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=519bp|location=Sequence derived from alignment at P-wetherbeei_contig55828:1..519- (Phaeothamnion wetherbeei SAG_119_79)
CGCATCGAGGCCGGCCAGCCGACGGTGTGCTCGGAGACCTGCGTCGGGCG
CATCCGCTACCTCGGCGTGGTTCTCTACGACGCCGATAGAATCGTCGACG
CCGCGAGCAAGCCGGATGAGCGCGATCTCTATCAGGCTCAGCTCGACGTG
TTCCTCAACCCGAACGACCCTGCTGTCATAGCGGAGGCGGAAATGCAGGG
CATTCCGCATGCGTGGCTCGAAGCTGCCACAAAATCGCCGATCTGGAAGA
TGGCGATGGAATGGAAGGTCGCGTTCCCGCTGCATCCGGAATATCGCACC
TTGCCGATGGTCTGGTATGTGCCGCCACTGTCGCCGATCACATCGGCGGC
GTCCGCCGGCAAGATCGGCCTCGACGGCGACATGCCCGACGTCAAATCGC
TGCGCATTCCGATGAAGTATCTCGCCAACCTGCTGACCGCCGGCGACGAG
AAGCCCGTTACGCTTGCGCTGGAGCGAATGATGGCGATGCGGTCCTACAT
GCGGGCGAAGACCATCGAC
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