mRNA_P-wetherbeei_contig54598.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig54598.1.1
Unique NamemRNA_P-wetherbeei_contig54598.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: UPI001C3CC685 (hypothetical protein n=1 Tax=Miltoncostaea oceani TaxID=2843216 RepID=UPI001C3CC685)

HSP 1 Score: 341 bits (875), Expect = 9.440e-116
Identity = 166/176 (94.32%), Postives = 170/176 (96.59%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSLWHEMG 528
            LHDGDE+EPWE VLTIRGDYSLFA LETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAY GDTVLAA++FADRFA  LHVTVLVDFENDSVRTALEVADALGPRLW VRLDTS+TMVDRSLWHEMG
Sbjct:   94 LHDGDEIEPWEPVLTIRGDYSLFASLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYAGDTVLAARKFADRFAEHLHVTVLVDFENDSVRTALEVADALGPRLWGVRLDTSNTMVDRSLWHEMG 269
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: UPI001C3CD0F5 (hypothetical protein n=1 Tax=Miltoncostaea marina TaxID=2843215 RepID=UPI001C3CD0F5)

HSP 1 Score: 335 bits (859), Expect = 2.540e-113
Identity = 162/176 (92.05%), Postives = 168/176 (95.45%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSLWHEMG 528
            LHDGDE+EPWE V+TI GDYSLFA+LETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHW VQTGDGWAAH+AGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTV AA+RFADRFA EL +TVLVDFENDSVRTALEVADALGPRLW VRLDTSSTMVDRSLW EMG
Sbjct:   94 LHDGDEIEPWETVMTITGDYSLFAHLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWHVQTGDGWAAHIAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVAAAERFADRFAHELAITVLVDFENDSVRTALEVADALGPRLWGVRLDTSSTMVDRSLWQEMG 269
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: A0A838FH65_9ACTN (Nicotinate phosphoribosyltransferase n=1 Tax=Thermoleophilaceae bacterium TaxID=2732252 RepID=A0A838FH65_9ACTN)

HSP 1 Score: 293 bits (749), Expect = 1.290e-96
Identity = 139/176 (78.98%), Postives = 160/176 (90.91%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSLWHEMG 528
            LH+GDE+ P+E+V+TI+GDY LFA+LET+YLG LARRTLI RNVR+VV AA GKPI++FPAR DHW VQTGDGWAAHVAGAIGVSTDAQASWWGG+G+GTVPHGLIAA+GGDTV AA  FADRF  E++VTVLVDF+NDSVRT+LEVADALG RLW VRLDTS T+VDR+LW+EMG
Sbjct:   95 LHEGDEIAPYESVMTIQGDYGLFAHLETVYLGCLARRTLIMRNVREVVDAAQGKPIIFFPARHDHWLVQTGDGWAAHVAGAIGVSTDAQASWWGGRGIGTVPHGLIAAFGGDTVAAATAFADRFHGEMNVTVLVDFDNDSVRTSLEVADALGERLWGVRLDTSGTLVDRALWNEMG 270
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: A0A7V9S1G6_9ACTN (Nicotinate phosphoribosyltransferase n=1 Tax=Thermoleophilaceae bacterium TaxID=2732252 RepID=A0A7V9S1G6_9ACTN)

HSP 1 Score: 286 bits (732), Expect = 4.850e-94
Identity = 138/176 (78.41%), Postives = 152/176 (86.36%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSLWHEMG 528
            LH+GDEV P+E V+TI GDYSLFA+LET+YLG LARRTL+  NVR+VV AAG KPIL+FPAR DHW VQTGDGWAAHVAGAIGVSTDAQASWWGG+G+GTVPH LIAAYGGDTV AA  F  RF  + +V VLVDFENDSVRTALEVADALG RLW VRLDTS  +VDR+LWHEMG
Sbjct:   95 LHEGDEVSPFETVMTIEGDYSLFAHLETVYLGCLARRTLVMSNVREVVEAAGDKPILFFPARHDHWLVQTGDGWAAHVAGAIGVSTDAQASWWGGRGVGTVPHALIAAYGGDTVAAADAFTRRFGDDTNVVVLVDFENDSVRTALEVADALGDRLWGVRLDTSDRLVDRALWHEMG 270
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: A0A1H6FNA8_THEAL (Nicotinate phosphoribosyltransferase n=1 Tax=Thermoleophilum album TaxID=29539 RepID=A0A1H6FNA8_THEAL)

HSP 1 Score: 286 bits (731), Expect = 7.090e-94
Identity = 136/176 (77.27%), Postives = 153/176 (86.93%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSLWHEMG 528
            LH+GDE+ PWE V+ I GDYSLFA+LET+YLG LARRTLI RNVR+VV AA GKPILYFPAR DHW VQTGDGWAAHVAGA+GVSTDAQASWWGG+GMGTVPH LIAA+GGDTV AA+ FA+R+  E++V VLVDF+ND  RTA+EVADALG +LW VRLDTS TMVDR LW EMG
Sbjct:   96 LHEGDEIAPWETVMLIEGDYSLFAHLETVYLGCLARRTLIMRNVREVVEAARGKPILYFPARHDHWLVQTGDGWAAHVAGALGVSTDAQASWWGGRGMGTVPHALIAAFGGDTVAAARAFAERYHREMNVVVLVDFQNDCCRTAVEVADALGEQLWGVRLDTSGTMVDRCLWDEMG 271
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: A0A6J4SYQ7_9ACTN (Nicotinate phosphoribosyltransferase (Fragment) n=1 Tax=uncultured Solirubrobacteraceae bacterium TaxID=1162706 RepID=A0A6J4SYQ7_9ACTN)

HSP 1 Score: 284 bits (726), Expect = 2.540e-93
Identity = 140/176 (79.55%), Postives = 152/176 (86.36%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSLWHEMG 528
            LH+GDE+ P E VL I GDY LF +LET+ LG LARRTL+ RNVR+VVAAA GKPILYFPAR DHW VQTGDGWAAHVAGAIGVSTDAQASWWGG+G+GTVPHGLIAAYGGDTV AA+ FA RFAAE +VTVLVDFENDSV TAL VA ALGP LW VRLDTS TMVDR+LW +MG
Sbjct:   89 LHEGDEIGPREPVLEITGDYGLFTHLETVVLGCLARRTLVMRNVREVVAAARGKPILYFPARHDHWLVQTGDGWAAHVAGAIGVSTDAQASWWGGRGIGTVPHGLIAAYGGDTVAAARAFAGRFAAETNVTVLVDFENDSVGTALAVAGALGPDLWGVRLDTSETMVDRALWEDMG 264
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: A0A838PJ36_9ACTN (Nicotinate phosphoribosyltransferase (Fragment) n=1 Tax=Thermoleophilaceae bacterium TaxID=2732252 RepID=A0A838PJ36_9ACTN)

HSP 1 Score: 276 bits (707), Expect = 2.250e-91
Identity = 134/176 (76.14%), Postives = 152/176 (86.36%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSLWHEMG 528
            LH+GDE+ P E V+TI GDYSLFA+LET+YLG LARRTL+  NVR+VV AA GKPIL+FPAR DHW VQTGDGWAAHVAGAIGVSTDAQASWWGG G+GTVPH LIAAYGGDTV A++ FA R+A E +VTVLVDF+NDSVRTA+EVADALG  LW VRLDTS  +VDR+LW +MG
Sbjct:   89 LHEGDEIAPHETVMTIEGDYSLFAHLETVYLGCLARRTLVMGNVREVVEAARGKPILFFPARHDHWLVQTGDGWAAHVAGAIGVSTDAQASWWGGTGIGTVPHALIAAYGGDTVEASRAFARRYAPETNVTVLVDFDNDSVRTAIEVADALGDDLWGVRLDTSERIVDRALWDDMG 264
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: UPI00041E1A8B (quinolinate phosphoribosyl transferase n=1 Tax=Solirubrobacter sp. URHD0082 TaxID=1298857 RepID=UPI00041E1A8B)

HSP 1 Score: 279 bits (714), Expect = 2.430e-91
Identity = 133/171 (77.78%), Postives = 151/171 (88.30%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSL 513
            LH+GDE+ PWE VLTI GDY+ FA+LET+YLGV+ARRTLI RNVR+VV AA GKPILYFPAR DHW VQTGDGWAAH+AGAIGVSTDAQASWWGG+G+GTVPHGLIAAYGGDT LAA++FADRF  +++VTVLVDFENDSV+TALEVA+ LG  LW VRLDTS  + D SL
Sbjct:   93 LHEGDEISPWETVLTIEGDYADFAHLETVYLGVMARRTLIMRNVREVVDAARGKPILYFPARHDHWLVQTGDGWAAHIAGAIGVSTDAQASWWGGRGVGTVPHGLIAAYGGDTALAARKFADRFKRDMNVTVLVDFENDSVKTALEVAEVLGEDLWGVRLDTSDKLADASL 263
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: A0A538KBY1_9ACTN (Nicotinate phosphoribosyltransferase n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A538KBY1_9ACTN)

HSP 1 Score: 279 bits (713), Expect = 2.520e-91
Identity = 136/171 (79.53%), Postives = 153/171 (89.47%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSL 513
            L++GDE+ P EAVLTIRGDYS FA+LET+YLG LARR+L+ RNV +VVAAA GK IL+FPAR DHW VQTGDGWAAHVAGAIGVSTDAQASWWGG+G+GTVPHGLIAAYGGDTV AA +FAD FA E++VTVLVDFENDSVRTALEVADALG RLW VRLDTS  +VD++L
Sbjct:   87 LYEGDEIGPREAVLTIRGDYSRFAHLETVYLGCLARRSLVMRNVAEVVAAANGKQILFFPARHDHWLVQTGDGWAAHVAGAIGVSTDAQASWWGGRGVGTVPHGLIAAYGGDTVAAATKFADHFAGEMNVTVLVDFENDSVRTALEVADALGERLWGVRLDTSEALVDKAL 257
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BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Match: UPI002010A7FA (quinolinate phosphoribosyl transferase n=1 Tax=Conexibacter sp. S30A1 TaxID=2937800 RepID=UPI002010A7FA)

HSP 1 Score: 278 bits (712), Expect = 5.040e-91
Identity = 136/176 (77.27%), Postives = 153/176 (86.93%), Query Frame = 1 
Query:    1 LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAAAGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGTVPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADALGPRLWAVRLDTSSTMVDRSLWHEMG 528
            L++GD +EPWE VLTI G+Y LFA+LET+YLG LARR+LI RNV  VV AA GKPILYFPAR DHW VQTGDGWAAHVAGAIGVSTDAQASWWGG+G+GTVPHGLIAAYGGDTV AA+ FA+RF AE+++TVLVDF+NDS RTA+EVA ALGP+LW VRLDTS  MVDRSL   MG
Sbjct:   94 LYEGDPIEPWETVLTIEGEYPLFAHLETVYLGSLARRSLIMRNVDAVVRAARGKPILYFPARHDHWHVQTGDGWAAHVAGAIGVSTDAQASWWGGRGVGTVPHGLIAAYGGDTVAAARVFAERFHAEMNITVLVDFDNDSPRTAVEVARALGPQLWGVRLDTSERMVDRSLIDSMG 269
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig54598.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI001C3CC6859.440e-11694.32hypothetical protein n=1 Tax=Miltoncostaea oceani ... [more]
UPI001C3CD0F52.540e-11392.05hypothetical protein n=1 Tax=Miltoncostaea marina ... [more]
A0A838FH65_9ACTN1.290e-9678.98Nicotinate phosphoribosyltransferase n=1 Tax=Therm... [more]
A0A7V9S1G6_9ACTN4.850e-9478.41Nicotinate phosphoribosyltransferase n=1 Tax=Therm... [more]
A0A1H6FNA8_THEAL7.090e-9477.27Nicotinate phosphoribosyltransferase n=1 Tax=Therm... [more]
A0A6J4SYQ7_9ACTN2.540e-9379.55Nicotinate phosphoribosyltransferase (Fragment) n=... [more]
A0A838PJ36_9ACTN2.250e-9176.14Nicotinate phosphoribosyltransferase (Fragment) n=... [more]
UPI00041E1A8B2.430e-9177.78quinolinate phosphoribosyl transferase n=1 Tax=Sol... [more]
A0A538KBY1_9ACTN2.520e-9179.53Nicotinate phosphoribosyltransferase n=1 Tax=Actin... [more]
UPI002010A7FA5.040e-9177.27quinolinate phosphoribosyl transferase n=1 Tax=Con... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig54598contigP-wetherbeei_contig54598:1..528 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog469383.Cwoe_5866
PFAMsIsochorismatase,QRPTase_C,QRPTase_N
Model size528
Max annot lvl84995|Rubrobacteria
KEGG rclassRC00033
KEGG koko:K00763
KEGG ReactionR01724
KEGG Pathwayko00760,ko01100,map00760,map01100
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.68e-90
EggNOG OGsCOG1488@1|root,COG1488@2|Bacteria,2GJAT@201174|Actinobacteria,4CQTG@84995|Rubrobacteria
EC6.3.4.21
DescriptionCatalyzes the synthesis of beta-nicotinate D- ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP
Cds size528
COG categoryH
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186815.3810816-CDS-P-wetherbeei_contig54598:0..5281683186815.3810816-CDS-P-wetherbeei_contig54598:0..528Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig54598 1..528 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig54598.1.1prot_P-wetherbeei_contig54598.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig54598 1..528 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig54598.1.1

>prot_P-wetherbeei_contig54598.1.1 ID=prot_P-wetherbeei_contig54598.1.1|Name=mRNA_P-wetherbeei_contig54598.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=176bp
LHDGDEVEPWEAVLTIRGDYSLFAYLETLYLGVLARRTLISRNVRDVVAA
AGGKPILYFPARFDHWQVQTGDGWAAHVAGAIGVSTDAQASWWGGKGMGT
VPHGLIAAYGGDTVLAAQRFADRFAAELHVTVLVDFENDSVRTALEVADA
LGPRLWAVRLDTSSTMVDRSLWHEMG
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mRNA from alignment at P-wetherbeei_contig54598:1..528-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig54598.1.1 ID=mRNA_P-wetherbeei_contig54598.1.1|Name=mRNA_P-wetherbeei_contig54598.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=528bp|location=Sequence derived from alignment at P-wetherbeei_contig54598:1..528- (Phaeothamnion wetherbeei SAG_119_79)
TTGCACGACGGCGACGAGGTGGAGCCGTGGGAGGCCGTGCTCACGATCCG
CGGCGACTACAGCCTGTTCGCCTATCTCGAGACGCTCTACCTCGGGGTGC
TGGCGCGGCGCACGCTGATCAGCCGCAACGTGCGCGATGTGGTGGCCGCG
GCCGGGGGCAAGCCGATCCTCTACTTCCCCGCCCGCTTCGACCACTGGCA
GGTGCAGACCGGAGACGGGTGGGCCGCCCACGTGGCCGGGGCCATCGGGG
TGTCCACCGACGCCCAGGCCTCGTGGTGGGGGGGCAAGGGCATGGGCACG
GTGCCGCACGGCCTCATCGCGGCCTACGGCGGCGACACGGTGCTCGCCGC
CCAGCGCTTCGCCGACCGCTTCGCGGCCGAGCTGCACGTCACCGTGCTGG
TGGATTTCGAGAACGACTCGGTGCGCACGGCGCTCGAGGTGGCCGACGCC
CTCGGGCCACGCCTGTGGGCGGTGCGCCTGGACACCTCCAGCACCATGGT
CGATCGCTCGCTCTGGCACGAGATGGGG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig54598:1..528-

>mRNA_P-wetherbeei_contig54598.1.1 ID=mRNA_P-wetherbeei_contig54598.1.1|Name=mRNA_P-wetherbeei_contig54598.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=528bp|location=Sequence derived from alignment at P-wetherbeei_contig54598:1..528- (Phaeothamnion wetherbeei SAG_119_79)
TTGCACGACGGCGACGAGGTGGAGCCGTGGGAGGCCGTGCTCACGATCCG
CGGCGACTACAGCCTGTTCGCCTATCTCGAGACGCTCTACCTCGGGGTGC
TGGCGCGGCGCACGCTGATCAGCCGCAACGTGCGCGATGTGGTGGCCGCG
GCCGGGGGCAAGCCGATCCTCTACTTCCCCGCCCGCTTCGACCACTGGCA
GGTGCAGACCGGAGACGGGTGGGCCGCCCACGTGGCCGGGGCCATCGGGG
TGTCCACCGACGCCCAGGCCTCGTGGTGGGGGGGCAAGGGCATGGGCACG
GTGCCGCACGGCCTCATCGCGGCCTACGGCGGCGACACGGTGCTCGCCGC
CCAGCGCTTCGCCGACCGCTTCGCGGCCGAGCTGCACGTCACCGTGCTGG
TGGATTTCGAGAACGACTCGGTGCGCACGGCGCTCGAGGTGGCCGACGCC
CTCGGGCCACGCCTGTGGGCGGTGCGCCTGGACACCTCCAGCACCATGGT
CGATCGCTCGCTCTGGCACGAGATGGGG
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