mRNA_P-wetherbeei_contig54187.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig54187.1.1
Unique NamemRNA_P-wetherbeei_contig54187.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: A0A5P6PGI6_9BRAD (Alpha/beta hydrolase n=7 Tax=Bradyrhizobium TaxID=374 RepID=A0A5P6PGI6_9BRAD)

HSP 1 Score: 330 bits (845), Expect = 9.960e-111
Identity = 171/177 (96.61%), Postives = 173/177 (97.74%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQGRMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPEILEQVARENAKGRRLL+VTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQG RFQEMHVDGGVTAPVLTLPEALLFQGR+P NTK  IYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR
Sbjct:  183 VARYVGPEILEQVARENAKGRRLLIVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGRRFQEMHVDGGVTAPVLTLPEALLFQGRLPGNTKSNIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 359
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BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: A0A5S4YNI0_9BRAD (Alpha/beta hydrolase n=2 Tax=Bradyrhizobium TaxID=374 RepID=A0A5S4YNI0_9BRAD)

HSP 1 Score: 322 bits (826), Expect = 5.690e-108
Identity = 167/177 (94.35%), Postives = 170/177 (96.05%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQGRMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPEIL QVARE  KGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQGR+P N KM+IYILVNKKIERNFELV+N TIDVASRSLSSITQSQTRSIIFSTYDFAKRN LGFHLSYIAR
Sbjct:  174 VARYVGPEILAQVAREYGKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQGRLPGNAKMDIYILVNKKIERNFELVANGTIDVASRSLSSITQSQTRSIIFSTYDFAKRNQLGFHLSYIAR 350
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BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: A0A5D3K2M0_9BRAD (Alpha/beta hydrolase n=27 Tax=Bradyrhizobium TaxID=374 RepID=A0A5D3K2M0_9BRAD)

HSP 1 Score: 322 bits (824), Expect = 1.380e-107
Identity = 166/177 (93.79%), Postives = 172/177 (97.18%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQGRMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPEI+ QVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQG RFQEMHVDGGVTAPVLTLPEALLFQGR+P N KM+IYILVNKKIERNFELV+NSTIDVASRSLSSITQSQTRSIIFSTYDFA+RN+LGFHLSYI R
Sbjct:  180 VARYVGPEIMAQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGRRFQEMHVDGGVTAPVLTLPEALLFQGRLPGNAKMDIYILVNKKIERNFELVANSTIDVASRSLSSITQSQTRSIIFSTYDFAQRNHLGFHLSYIER 356
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BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: A0A176Y7I3_9BRAD (Alpha/beta hydrolase n=1 Tax=Bradyrhizobium centrolobii TaxID=1505087 RepID=A0A176Y7I3_9BRAD)

HSP 1 Score: 321 bits (823), Expect = 1.960e-107
Identity = 167/177 (94.35%), Postives = 170/177 (96.05%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQGRMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPEIL QVARENAKGR+LLVVTTDLDTQRTAIWDMGKIAAVGTPEAL LFRDVMAASASIPLVFPPILIEAEGQG RFQEMHVDGGVTAPVLTLPEALLFQGR+P N KM+IYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRN LGFHLSYI R
Sbjct:  180 VARYVGPEILAQVARENAKGRKLLVVTTDLDTQRTAIWDMGKIAAVGTPEALHLFRDVMAASASIPLVFPPILIEAEGQGRRFQEMHVDGGVTAPVLTLPEALLFQGRLPGNAKMDIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNRLGFHLSYIER 356
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BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: UPI00190C8E12 (patatin-like phospholipase family protein n=1 Tax=Bradyrhizobium diazoefficiens TaxID=1355477 RepID=UPI00190C8E12)

HSP 1 Score: 320 bits (819), Expect = 6.770e-107
Identity = 165/177 (93.22%), Postives = 171/177 (96.61%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQGRMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPEIL QVARENAKGRRLL+VTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGH+FQEMHVDGGVTAPVLTLPEALL Q R+P N KM+IYILVNKKIERNFELV+NSTIDVASRSLSSITQSQTRSIIFSTYDFAKRN+LGFHLSYI R
Sbjct:  175 VARYVGPEILAQVARENAKGRRLLIVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHKFQEMHVDGGVTAPVLTLPEALLSQDRLPGNAKMDIYILVNKKIERNFELVANSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNHLGFHLSYIER 351
[Close]
BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: A0A4Y9KXE8_9BRAD (Alpha/beta hydrolase (Fragment) n=1 Tax=Bradyrhizobium frederickii TaxID=2560054 RepID=A0A4Y9KXE8_9BRAD)

HSP 1 Score: 310 bits (793), Expect = 1.160e-104
Identity = 160/178 (89.89%), Postives = 170/178 (95.51%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQG-RMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPEIL QVARENAKGR+LL+VTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPI+I+AEGQG +FQEMHVDGGVTAPVLTLPEALLFQG R+P + K +IYILVNKKIERNFELVSN TIDVASRSLS+ITQSQTRSIIFSTYDFA+RN LGFHLSYIAR
Sbjct:   54 VARYVGPEILAQVARENAKGRKLLIVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPIMIDAEGQGRKFQEMHVDGGVTAPVLTLPEALLFQGSRLPGSAKFDIYILVNKKIERNFELVSNGTIDVASRSLSAITQSQTRSIIFSTYDFARRNRLGFHLSYIAR 231
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BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: A0A844S6Z0_9BRAD (Alpha/beta hydrolase n=7 Tax=Bradyrhizobium TaxID=374 RepID=A0A844S6Z0_9BRAD)

HSP 1 Score: 313 bits (803), Expect = 2.810e-104
Identity = 163/178 (91.57%), Postives = 171/178 (96.07%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQ-GRMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPEI+ QVARENAKGRRLLVVTTDLDTQRTAIWDMG+IAAVGTPEALKLFRDVMAASASIPLVFPPI+I+AEG G +FQEMHVDGGVTAPVLTLPEALLFQ GR+P NTKM+IYILVNKKIERNFELVSN TIDVASRSLS+ITQSQTRSIIFSTYDFAKRN LGFHLSYIAR
Sbjct:  188 VARYVGPEIMAQVARENAKGRRLLVVTTDLDTQRTAIWDMGRIAAVGTPEALKLFRDVMAASASIPLVFPPIMIDAEGGGRKFQEMHVDGGVTAPVLTLPEALLFQSGRLPGNTKMDIYILVNKKIERNFELVSNGTIDVASRSLSAITQSQTRSIIFSTYDFAKRNRLGFHLSYIAR 365
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BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: H5YHD9_9BRAD (Putative esterase of the alpha-beta hydrolase superfamily n=38 Tax=Bradyrhizobiaceae TaxID=41294 RepID=H5YHD9_9BRAD)

HSP 1 Score: 313 bits (801), Expect = 5.640e-104
Identity = 163/178 (91.57%), Postives = 171/178 (96.07%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQG-RMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPEI+ QVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPI+I+AEG+G RFQEMHVDGGVTAPVLTLPEALLFQG R+P   K++IYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFA+RN LGFHLSYIAR
Sbjct:  188 VARYVGPEIMAQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPIMIDAEGEGRRFQEMHVDGGVTAPVLTLPEALLFQGSRLPGPAKLDIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFARRNRLGFHLSYIAR 365
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BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: A0A160ULX7_BRASZ (PNPLA domain-containing protein n=166 Tax=Bradyrhizobiaceae TaxID=41294 RepID=A0A160ULX7_BRASZ)

HSP 1 Score: 310 bits (795), Expect = 4.570e-103
Identity = 161/178 (90.45%), Postives = 171/178 (96.07%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQG-RMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPE++ QVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPI+I+AEG+G RFQEMHVDGGVTAPVLTLPEALLFQG R+P   K++IYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFA+RN LGFHLS+IAR
Sbjct:  188 VARYVGPELMAQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPIMIDAEGEGRRFQEMHVDGGVTAPVLTLPEALLFQGSRLPGPAKLDIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFARRNRLGFHLSFIAR 365
[Close]
BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Match: A0A2U8PU96_9BRAD (Alpha/beta hydrolase n=1 Tax=Bradyrhizobium amphicarpaeae TaxID=1404768 RepID=A0A2U8PU96_9BRAD)

HSP 1 Score: 310 bits (793), Expect = 9.180e-103
Identity = 159/178 (89.33%), Postives = 171/178 (96.07%), Query Frame = 1 
Query:    1 VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPEALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLPEALLFQG-RMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQSQTRSIIFSTYDFAKRNNLGFHLSYIAR 531
            VARYVGPE++ QVARENAKGR+LLVVTTDLDTQRTAIWDMGKIAAVGTP+ALKLFRDVMAASASIPLVFPPI+I+AEG+G +FQEMHVDGGVTAPVLTLPEALLFQG R+P + KM+IYILVNKKIERNFELVSN TIDVASRSLS+ITQSQTRSIIFSTYDFAKRN LGFHLSYIAR
Sbjct:  188 VARYVGPEVMAQVARENAKGRKLLVVTTDLDTQRTAIWDMGKIAAVGTPQALKLFRDVMAASASIPLVFPPIMIDAEGEGRKFQEMHVDGGVTAPVLTLPEALLFQGSRLPGSAKMDIYILVNKKIERNFELVSNGTIDVASRSLSAITQSQTRSIIFSTYDFAKRNRLGFHLSYIAR 365
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig54187.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5P6PGI6_9BRAD9.960e-11196.61Alpha/beta hydrolase n=7 Tax=Bradyrhizobium TaxID=... [more]
A0A5S4YNI0_9BRAD5.690e-10894.35Alpha/beta hydrolase n=2 Tax=Bradyrhizobium TaxID=... [more]
A0A5D3K2M0_9BRAD1.380e-10793.79Alpha/beta hydrolase n=27 Tax=Bradyrhizobium TaxID... [more]
A0A176Y7I3_9BRAD1.960e-10794.35Alpha/beta hydrolase n=1 Tax=Bradyrhizobium centro... [more]
UPI00190C8E126.770e-10793.22patatin-like phospholipase family protein n=1 Tax=... [more]
A0A4Y9KXE8_9BRAD1.160e-10489.89Alpha/beta hydrolase (Fragment) n=1 Tax=Bradyrhizo... [more]
A0A844S6Z0_9BRAD2.810e-10491.57Alpha/beta hydrolase n=7 Tax=Bradyrhizobium TaxID=... [more]
H5YHD9_9BRAD5.640e-10491.57Putative esterase of the alpha-beta hydrolase supe... [more]
A0A160ULX7_BRASZ4.570e-10390.45PNPLA domain-containing protein n=166 Tax=Bradyrhi... [more]
A0A2U8PU96_9BRAD9.180e-10389.33Alpha/beta hydrolase n=1 Tax=Bradyrhizobium amphic... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig54187contigP-wetherbeei_contig54187:3..533 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog335659.S23_34700
Preferred nameMA20_41185
PFAMsPatatin
Model size531
Max annot lvl28211|Alphaproteobacteria
Hectar predicted targeting categoryother localisation
Exons1
Evalue4.7e-112
EggNOG OGsCOG1752@1|root,COG1752@2|Bacteria,1NT1Q@1224|Proteobacteria,2TRM3@28211|Alphaproteobacteria,3JRE2@41294|Bradyrhizobiaceae
DescriptionPatatin-like phospholipase
Cds size531
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186809.972733-CDS-P-wetherbeei_contig54187:2..5331683186809.972733-CDS-P-wetherbeei_contig54187:2..533Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig54187 3..533 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig54187.1.1prot_P-wetherbeei_contig54187.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig54187 3..533 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig54187.1.1

>prot_P-wetherbeei_contig54187.1.1 ID=prot_P-wetherbeei_contig54187.1.1|Name=mRNA_P-wetherbeei_contig54187.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=177bp
VARYVGPEILEQVARENAKGRRLLVVTTDLDTQRTAIWDMGKIAAVGTPE
ALKLFRDVMAASASIPLVFPPILIEAEGQGHRFQEMHVDGGVTAPVLTLP
EALLFQGRMPANTKMEIYILVNKKIERNFELVSNSTIDVASRSLSSITQS
QTRSIIFSTYDFAKRNNLGFHLSYIAR
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mRNA from alignment at P-wetherbeei_contig54187:3..533-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig54187.1.1 ID=mRNA_P-wetherbeei_contig54187.1.1|Name=mRNA_P-wetherbeei_contig54187.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=531bp|location=Sequence derived from alignment at P-wetherbeei_contig54187:3..533- (Phaeothamnion wetherbeei SAG_119_79)
GTGGCACGCTATGTCGGGCCGGAGATCCTGGAGCAGGTCGCCCGCGAAAA
TGCCAAGGGCCGCAGGCTGCTGGTGGTGACGACCGATCTCGACACCCAGC
GAACCGCCATCTGGGACATGGGCAAGATCGCCGCCGTGGGGACGCCCGAG
GCGCTCAAGCTGTTTCGCGACGTGATGGCGGCTTCCGCCAGCATTCCGCT
GGTATTTCCGCCCATCCTGATCGAAGCCGAAGGCCAGGGACACAGATTTC
AGGAGATGCATGTCGACGGCGGCGTGACGGCGCCGGTGCTGACGCTGCCG
GAGGCCCTGCTCTTCCAGGGGCGCATGCCGGCCAACACGAAGATGGAGAT
CTACATCCTCGTCAATAAGAAGATCGAACGCAACTTCGAGCTGGTCTCCA
ACAGCACCATTGACGTCGCCTCGCGCAGCCTGTCGTCGATCACCCAGTCG
CAGACGCGCTCGATCATCTTCTCGACCTATGATTTCGCCAAGCGCAACAA
TCTGGGCTTCCACCTCTCCTATATCGCGCGC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig54187:3..533-

>mRNA_P-wetherbeei_contig54187.1.1 ID=mRNA_P-wetherbeei_contig54187.1.1|Name=mRNA_P-wetherbeei_contig54187.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=531bp|location=Sequence derived from alignment at P-wetherbeei_contig54187:3..533- (Phaeothamnion wetherbeei SAG_119_79)
GTGGCACGCTATGTCGGGCCGGAGATCCTGGAGCAGGTCGCCCGCGAAAA
TGCCAAGGGCCGCAGGCTGCTGGTGGTGACGACCGATCTCGACACCCAGC
GAACCGCCATCTGGGACATGGGCAAGATCGCCGCCGTGGGGACGCCCGAG
GCGCTCAAGCTGTTTCGCGACGTGATGGCGGCTTCCGCCAGCATTCCGCT
GGTATTTCCGCCCATCCTGATCGAAGCCGAAGGCCAGGGACACAGATTTC
AGGAGATGCATGTCGACGGCGGCGTGACGGCGCCGGTGCTGACGCTGCCG
GAGGCCCTGCTCTTCCAGGGGCGCATGCCGGCCAACACGAAGATGGAGAT
CTACATCCTCGTCAATAAGAAGATCGAACGCAACTTCGAGCTGGTCTCCA
ACAGCACCATTGACGTCGCCTCGCGCAGCCTGTCGTCGATCACCCAGTCG
CAGACGCGCTCGATCATCTTCTCGACCTATGATTTCGCCAAGCGCAACAA
TCTGGGCTTCCACCTCTCCTATATCGCGCGC
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