mRNA_P-wetherbeei_contig53497.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig53497.1.1
Unique NamemRNA_P-wetherbeei_contig53497.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A382QDP2_9ZZZZ (Uncharacterized protein n=1 Tax=marine metagenome TaxID=408172 RepID=A0A382QDP2_9ZZZZ)

HSP 1 Score: 303 bits (775), Expect = 1.300e-101
Identity = 145/179 (81.01%), Postives = 159/179 (88.83%), Query Frame = 1 
Query:    1 LGGTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLVPLADL 537
            L GTAEA GTVAKVGEYMR+ DDDPDVRVIVLTG+GRGFCSGADLK R +  E+GP   GARGSHEGPDAARQHF++G TKLHRDIS+IRKPTIAMING AVG+GMDM+LHCDIRIGCEATRF+ YQQ GQIIENGGCYYLPKM+GLGRALEFAYTG L  + AY+WGMLNHLV L +L
Sbjct:   37 LYGTAEALGTVAKVGEYMRAGDDDPDVRVIVLTGVGRGFCSGADLKTRPSKDEVGPSVDGARGSHEGPDAARQHFYHGFTKLHRDISIIRKPTIAMINGAAVGSGMDMALHCDIRIGCEATRFIGYQQAGQIIENGGCYYLPKMVGLGRALEFAYTGMLSADLAYQWGMLNHLVSLEEL 215
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A2E4XZ68_9PROT (Uncharacterized protein n=2 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A2E4XZ68_9PROT)

HSP 1 Score: 298 bits (763), Expect = 7.340e-100
Identity = 144/179 (80.45%), Postives = 157/179 (87.71%), Query Frame = 1 
Query:    1 LGGTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLVPLADL 537
            L GTAE NGTVAKVGEYMR+ADDDPDVRVIVLTG+GRGFCSGADLK R +  EIGP   GARGSHEGPDA RQHF++G TKLHRDIS+IRKPTIAMING AVG+GMDMSLHCDIRIGCE TRF+ YQQ GQI+ENGG YYLPKM+GLGRALEFAYTGSLD + AY WG+LNHLV   +L
Sbjct:   32 LYGTAENNGTVAKVGEYMRAADDDPDVRVIVLTGVGRGFCSGADLKERPSEDEIGPNVEGARGSHEGPDAMRQHFYHGFTKLHRDISVIRKPTIAMINGAAVGSGMDMSLHCDIRIGCEKTRFIGYQQAGQIMENGGSYYLPKMVGLGRALEFAYTGSLDADRAYEWGLLNHLVSSEEL 210
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A2E9Y4M1_9PROT (Uncharacterized protein n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A2E9Y4M1_9PROT)

HSP 1 Score: 295 bits (754), Expect = 2.020e-98
Identity = 142/179 (79.33%), Postives = 155/179 (86.59%), Query Frame = 1 
Query:    1 LGGTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLVPLADL 537
            L GTAE  GTVAKVGEYMR+ DDDPDVRVIVLTG+GRGFCSGADLK R +A E GP+ +GARG HEGP AARQHF++G TKLHRDIS+IRKPTIAMING AVG+GMDM+LHCDIRIGCE TRF+ YQQ GQIIENGG YYLPKM+GLGRALEF YTGSL  E AY WGMLNHLV L +L
Sbjct:   37 LYGTAEEMGTVAKVGEYMRAGDDDPDVRVIVLTGVGRGFCSGADLKSRPSADEXGPDFVGARGXHEGPXAARQHFYHGFTKLHRDISIIRKPTIAMINGAAVGSGMDMALHCDIRIGCENTRFIGYQQAGQIIENGGSYYLPKMVGLGRALEFVYTGSLSAERAYEWGMLNHLVTLDEL 215
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A5C8PDP5_9HYPH (Uncharacterized protein n=2 Tax=Vineibacter terrae TaxID=2586908 RepID=A0A5C8PDP5_9HYPH)

HSP 1 Score: 287 bits (735), Expect = 1.360e-94
Identity = 138/179 (77.09%), Postives = 152/179 (84.92%), Query Frame = 1 
Query:    1 LGGTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLVPLADL 537
            L GTAE NGTV+KVGEYMR+ DDDPD+RVIVLTG+GR FC+GADL+ R +    GP   G RG HEGPDAAR+HFF+GLTKLHRDISLIRKPTIAMINGPAVGAGMDM+LHCDIR+GCE TRFVAY   GQIIENGG YYLPKM+GLGRALEFAYTG LD E A+ WG+LNHLVP   L
Sbjct:  104 LVGTAEENGTVSKVGEYMRAGDDDPDIRVIVLTGVGRAFCAGADLRSRPSPDVTGPNFPGNRGPHEGPDAAREHFFHGLTKLHRDISLIRKPTIAMINGPAVGAGMDMALHCDIRMGCEKTRFVAYHNAGQIIENGGSYYLPKMVGLGRALEFAYTGELDAERAHAWGLLNHLVPSEQL 282
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A259MUA6_9PROT (Uncharacterized protein n=3 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A259MUA6_9PROT)

HSP 1 Score: 282 bits (722), Expect = 1.250e-93
Identity = 137/174 (78.74%), Postives = 148/174 (85.06%), Query Frame = 1 
Query:    1 LGGTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLV 522
            L GTAE NGTVAKVGEYMR+ DDDP++RVIVLTG GR FC+GADL+    A   G E  G RGSHEGPDAARQHFF+G TKLHRDISLIRKPTIAMINGPAVG+GMDM+LHCDIRIGCE TRF+ YQ  GQIIENGG YYLPKM+GLGRALEFAYTG LD + AY WGMLNHLV
Sbjct:   32 LVGTAEENGTVAKVGEYMRAGDDDPNIRVIVLTGAGRVFCAGADLRSNMPADVTGEEFSGNRGSHEGPDAARQHFFHGFTKLHRDISLIRKPTIAMINGPAVGSGMDMALHCDIRIGCEKTRFIGYQNAGQIIENGGSYYLPKMVGLGRALEFAYTGELDAQRAYDWGMLNHLV 205
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A2E5PN93_9PROT (Uncharacterized protein n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A2E5PN93_9PROT)

HSP 1 Score: 276 bits (706), Expect = 3.370e-91
Identity = 131/179 (73.18%), Postives = 149/179 (83.24%), Query Frame = 1 
Query:    1 LGGTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLVPLADL 537
            L G +E N TVAKVGEYMR+ADDDPD+R+IVLTG+GR FC+GA+LK +A +   G   +G R   +G DA RQHFF+G TKLHRDI+ IRKPTIAMINGPAVG+GMDMSLHCD+RIGCE TRF+AY Q GQIIENGGCYYLP MIGLGRALEFAYTGS D ETAYRWGM+NHLV   +L
Sbjct:   32 LVGNSEENSTVAKVGEYMRAADDDPDIRIIVLTGVGRAFCAGANLKKKAPSEITGENFVGNRSGRDGADAVRQHFFHGFTKLHRDIADIRKPTIAMINGPAVGSGMDMSLHCDVRIGCEKTRFIAYHQAGQIIENGGCYYLPNMIGLGRALEFAYTGSCDAETAYRWGMINHLVDAENL 210
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A2S6U1G0_9PROT (Carnitinyl-CoA dehydratase n=1 Tax=Alphaproteobacteria bacterium MarineAlpha4_Bin1 TaxID=2013074 RepID=A0A2S6U1G0_9PROT)

HSP 1 Score: 273 bits (698), Expect = 5.540e-90
Identity = 131/172 (76.16%), Postives = 144/172 (83.72%), Query Frame = 1 
Query:    7 GTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLV 522
            G ++ N TVAKVGEYMR+ DDDPD+RVIVLTG+GRGFCSGADLKG A     G   IG R S EGPD  RQHFF+G TKLHRDI+ IRKPTIAMINGPAVG+GMDM+LHCDIRIGCE TRFV YQQ GQIIENGG YYLP+M+GLGRALEFAYTG LD + AY WG+LNHLV
Sbjct:   34 GNSQENSTVAKVGEYMRAGDDDPDLRVIVLTGVGRGFCSGADLKGTAPPEVTGENFIGNRSSREGPDGVRQHFFHGFTKLHRDIADIRKPTIAMINGPAVGSGMDMALHCDIRIGCEKTRFVGYQQAGQIIENGGSYYLPRMVGLGRALEFAYTGHLDAQRAYDWGILNHLV 205
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A6N8ZBR1_9CHLR (Uncharacterized protein n=2 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A6N8ZBR1_9CHLR)

HSP 1 Score: 273 bits (698), Expect = 7.430e-90
Identity = 129/177 (72.88%), Postives = 150/177 (84.75%), Query Frame = 1 
Query:    7 GTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLVPLADL 537
            G++E NGTVAKVGEYMR+ DDDP+VRVIVLTG+GRGFCSGAD++     +  G   +G RG+  GPDAAR+HF++G TKLHRDIS +RKPTIAMINGPAVG+GMDM+LHCDIR+GCE TRF+ YQQ+GQIIENGG YYLPKM+GLGRALEFAYTG LD E AY WGMLN+LV   DL
Sbjct:   43 GSSEENGTVAKVGEYMRAGDDDPNVRVIVLTGVGRGFCSGADMRRDNDPSVTGENFVGNRGTQPGPDAAREHFYHGFTKLHRDISQVRKPTIAMINGPAVGSGMDMALHCDIRLGCENTRFIGYQQLGQIIENGGSYYLPKMVGLGRALEFAYTGHLDAERAYAWGMLNYLVASEDL 219
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A2N0LDD4_9CHLR (Uncharacterized protein n=1 Tax=SAR202 cluster bacterium Io17-Chloro-G4 TaxID=1926613 RepID=A0A2N0LDD4_9CHLR)

HSP 1 Score: 270 bits (689), Expect = 1.290e-88
Identity = 128/179 (71.51%), Postives = 149/179 (83.24%), Query Frame = 1 
Query:    1 LGGTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLVPLADL 537
            L G ++ NGTVAKVGEYMR+ DDDP++RVIVLTG+GRGFCSGAD++     +  G   IG RGS EGPDAAR+HFF+G TKLHRDIS +RKPTIAMINGPAVG+GMDM+LHCDIR+GCE TRF+ YQQ+GQI ENGG YYLPKM GLGRALEFAYTG LD + AY WG+LN+LV   +L
Sbjct:   32 LVGNSQENGTVAKVGEYMRAGDDDPNIRVIVLTGVGRGFCSGADMRRDNDPSVTGENFIGNRGSEEGPDAAREHFFHGFTKLHRDISQVRKPTIAMINGPAVGSGMDMALHCDIRLGCENTRFIGYQQLGQIFENGGSYYLPKMAGLGRALEFAYTGHLDAQRAYDWGLLNYLVSAEEL 210
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BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Match: A0A3N5N802_9PROT (Uncharacterized protein n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A3N5N802_9PROT)

HSP 1 Score: 264 bits (675), Expect = 1.720e-86
Identity = 130/173 (75.14%), Postives = 148/173 (85.55%), Query Frame = 1 
Query:    7 GTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRATAAEI-GPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGPAVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRALEFAYTGSLDNETAYRWGMLNHLV 522
            G+AE NGTVAKVGEYMR+ DDDP++RVIVLTG+GRGFCSGAD+K R T AE+ G   +G R   +G D AR+HFF+G TKLHRDIS IRKPTIAMING AVG+GMDM+LHCDIRIGCE TRFV YQQ+GQI+ENGG YYLPKM+GLGRALEFAYTG LD + AY WG+LNHLV
Sbjct:   34 GSAEENGTVAKVGEYMRAGDDDPNIRVIVLTGVGRGFCSGADMK-RDTPAEMKGENFVGNRNIPDGGDHAREHFFHGFTKLHRDISQIRKPTIAMINGAAVGSGMDMTLHCDIRIGCENTRFVGYQQLGQIMENGGSYYLPKMVGLGRALEFAYTGQLDAKRAYEWGLLNHLV 205
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig53497.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A382QDP2_9ZZZZ1.300e-10181.01Uncharacterized protein n=1 Tax=marine metagenome ... [more]
A0A2E4XZ68_9PROT7.340e-10080.45Uncharacterized protein n=2 Tax=Rhodospirillaceae ... [more]
A0A2E9Y4M1_9PROT2.020e-9879.33Uncharacterized protein n=1 Tax=Rhodospirillaceae ... [more]
A0A5C8PDP5_9HYPH1.360e-9477.09Uncharacterized protein n=2 Tax=Vineibacter terrae... [more]
A0A259MUA6_9PROT1.250e-9378.74Uncharacterized protein n=3 Tax=Alphaproteobacteri... [more]
A0A2E5PN93_9PROT3.370e-9173.18Uncharacterized protein n=1 Tax=Rhodospirillaceae ... [more]
A0A2S6U1G0_9PROT5.540e-9076.16Carnitinyl-CoA dehydratase n=1 Tax=Alphaproteobact... [more]
A0A6N8ZBR1_9CHLR7.430e-9072.88Uncharacterized protein n=2 Tax=Chloroflexi bacter... [more]
A0A2N0LDD4_9CHLR1.290e-8871.51Uncharacterized protein n=1 Tax=SAR202 cluster bac... [more]
A0A3N5N802_9PROT1.720e-8675.14Uncharacterized protein n=1 Tax=Betaproteobacteria... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig53497contigP-wetherbeei_contig53497:2..538 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1051632.TPY_3801
PFAMsECH_1
Model size537
Max annot lvl186801|Clostridia
KEGG rclassRC00004,RC00326,RC02689,RC03003
KEGG koko:K15866
KEGG ReactionR09837,R09839
KEGG Pathwayko00360,ko01120,map00360,map01120
Hectar predicted targeting categoryother localisation
Exons1
Evalue2.96e-31
EggNOG OGsCOG1024@1|root,COG1024@2|Bacteria,1TQ2V@1239|Firmicutes,24HM8@186801|Clostridia,3WDT7@538999|Clostridiales incertae sedis
EC5.3.3.18
DescriptionBelongs to the enoyl-CoA hydratase isomerase family
Cds size537
COG categoryI
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186800.0762024-CDS-P-wetherbeei_contig53497:1..5381683186800.0762024-CDS-P-wetherbeei_contig53497:1..538Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig53497 2..538 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig53497.1.1prot_P-wetherbeei_contig53497.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig53497 2..538 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig53497.1.1

>prot_P-wetherbeei_contig53497.1.1 ID=prot_P-wetherbeei_contig53497.1.1|Name=mRNA_P-wetherbeei_contig53497.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=179bp
LGGTAEANGTVAKVGEYMRSADDDPDVRVIVLTGIGRGFCSGADLKGRAT
AAEIGPEAIGARGSHEGPDAARQHFFYGLTKLHRDISLIRKPTIAMINGP
AVGAGMDMSLHCDIRIGCEATRFVAYQQVGQIIENGGCYYLPKMIGLGRA
LEFAYTGSLDNETAYRWGMLNHLVPLADL
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mRNA from alignment at P-wetherbeei_contig53497:2..538-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig53497.1.1 ID=mRNA_P-wetherbeei_contig53497.1.1|Name=mRNA_P-wetherbeei_contig53497.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=537bp|location=Sequence derived from alignment at P-wetherbeei_contig53497:2..538- (Phaeothamnion wetherbeei SAG_119_79)
CTGGGCGGCACCGCGGAGGCGAACGGCACGGTCGCCAAGGTCGGCGAATA
CATGCGCTCCGCCGACGACGACCCGGATGTGCGCGTCATCGTGCTGACCG
GAATAGGTCGCGGCTTCTGCTCGGGTGCCGACCTGAAGGGCCGGGCAACC
GCCGCCGAGATCGGACCGGAGGCGATCGGCGCGCGCGGCAGCCACGAGGG
TCCCGACGCCGCGCGGCAGCACTTCTTCTACGGTCTCACCAAGCTGCACC
GCGACATCTCGCTGATCCGCAAACCGACGATCGCCATGATCAACGGTCCC
GCCGTCGGCGCGGGCATGGACATGTCGCTGCACTGTGACATCCGCATCGG
ATGCGAGGCGACGCGCTTCGTCGCCTATCAGCAGGTCGGCCAGATCATCG
AGAACGGCGGTTGCTACTACCTGCCGAAGATGATCGGCCTCGGGCGGGCG
CTCGAGTTCGCCTACACCGGCTCGCTCGACAACGAAACCGCCTATCGCTG
GGGCATGCTCAATCATCTCGTGCCGCTCGCCGATCTC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig53497:2..538-

>mRNA_P-wetherbeei_contig53497.1.1 ID=mRNA_P-wetherbeei_contig53497.1.1|Name=mRNA_P-wetherbeei_contig53497.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=537bp|location=Sequence derived from alignment at P-wetherbeei_contig53497:2..538- (Phaeothamnion wetherbeei SAG_119_79)
CTGGGCGGCACCGCGGAGGCGAACGGCACGGTCGCCAAGGTCGGCGAATA
CATGCGCTCCGCCGACGACGACCCGGATGTGCGCGTCATCGTGCTGACCG
GAATAGGTCGCGGCTTCTGCTCGGGTGCCGACCTGAAGGGCCGGGCAACC
GCCGCCGAGATCGGACCGGAGGCGATCGGCGCGCGCGGCAGCCACGAGGG
TCCCGACGCCGCGCGGCAGCACTTCTTCTACGGTCTCACCAAGCTGCACC
GCGACATCTCGCTGATCCGCAAACCGACGATCGCCATGATCAACGGTCCC
GCCGTCGGCGCGGGCATGGACATGTCGCTGCACTGTGACATCCGCATCGG
ATGCGAGGCGACGCGCTTCGTCGCCTATCAGCAGGTCGGCCAGATCATCG
AGAACGGCGGTTGCTACTACCTGCCGAAGATGATCGGCCTCGGGCGGGCG
CTCGAGTTCGCCTACACCGGCTCGCTCGACAACGAAACCGCCTATCGCTG
GGGCATGCTCAATCATCTCGTGCCGCTCGCCGATCTC
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