mRNA_P-wetherbeei_contig52707.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig52707.1.1
Unique NamemRNA_P-wetherbeei_contig52707.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: A0A1F4F1T5_9PROT (Peptide ABC transporter permease n=5 Tax=unclassified Betaproteobacteria TaxID=33809 RepID=A0A1F4F1T5_9PROT)

HSP 1 Score: 327 bits (837), Expect = 1.530e-109
Identity = 168/181 (92.82%), Postives = 175/181 (96.69%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRDLLSRI+YGTRISLTVGLLGILVSF+IGITLGGISGY+GGW+DN+IQRFIEMVRSFPELPLWMALSAALPV WSP+LVYFGITIILGLLDWPGLAR+VRSKLLALREEDFA AAVLMGA P RVI RHLLPSFASHLIASVTLSVP MILGETALSFLGLGLRPPVTSWGVLLNEAQN
Sbjct:  170 LGRDLLSRILYGTRISLTVGLLGILVSFVIGITLGGISGYYGGWLDNLIQRFIEMVRSFPELPLWMALSAALPVNWSPLLVYFGITIILGLLDWPGLARAVRSKLLALREEDFAAAAVLMGAGPGRVIGRHLLPSFASHLIASVTLSVPGMILGETALSFLGLGLRPPVTSWGVLLNEAQN 350
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: A0A536WL82_9PROT (ABC transporter permease n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A536WL82_9PROT)

HSP 1 Score: 325 bits (834), Expect = 4.360e-109
Identity = 166/181 (91.71%), Postives = 174/181 (96.13%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRDL SRIVYGTRISLTVGL GI+VSF+IG+TLGGISGY+GGWID VIQRFIEMVRSFPELPLWMALSAALPV WSPILVYFGITIILGLLDWPGLAR+VRSKLLALREEDFA AA+LMGASPARVI RHLLPSFASHLIAS TLSVP+MILGETALSFLGLGLRPP+TSWGVLLNEAQN
Sbjct:  165 LGRDLFSRIVYGTRISLTVGLFGIVVSFIIGLTLGGISGYYGGWIDTVIQRFIEMVRSFPELPLWMALSAALPVNWSPILVYFGITIILGLLDWPGLARAVRSKLLALREEDFATAAILMGASPARVIGRHLLPSFASHLIASATLSVPAMILGETALSFLGLGLRPPITSWGVLLNEAQN 345
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: UPI0019B9F062 (ABC transporter permease n=1 Tax=Rhodoferax sp. TaxID=50421 RepID=UPI0019B9F062)

HSP 1 Score: 323 bits (827), Expect = 5.190e-108
Identity = 165/181 (91.16%), Postives = 174/181 (96.13%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRDLLSRI+YGTRISLTVGLLGI VSF+IGITLGGISGY+GGWID VIQR IE+VRSFPELPLWMALSAALPVTWSPIL+YFGITIILG+LDWPGLAR+VRSK LALREEDFA AAVLMGASPARVI RHLLPSF SHLIAS+TLSVPSMILGETALSFLGLGLRPP+TSWGVLLNEAQN
Sbjct:  171 LGRDLLSRIIYGTRISLTVGLLGIAVSFVIGITLGGISGYYGGWIDTVIQRLIEVVRSFPELPLWMALSAALPVTWSPILIYFGITIILGMLDWPGLARAVRSKFLALREEDFAAAAVLMGASPARVIGRHLLPSFTSHLIASLTLSVPSMILGETALSFLGLGLRPPITSWGVLLNEAQN 351
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: A0A0S8B4T1_9PROT (Peptide ABC transporter permease n=1 Tax=Betaproteobacteria bacterium SG8_39 TaxID=1703390 RepID=A0A0S8B4T1_9PROT)

HSP 1 Score: 322 bits (825), Expect = 1.040e-107
Identity = 164/181 (90.61%), Postives = 172/181 (95.03%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRDLLSRI+YGTRISLTVGL GI+VSF IGI LGGISGY+GGWIDN+IQRFIEMVR+FPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLAR+VRSKLLALREEDFA AAVLMGA P RVI RHLLPSFASHLIAS TL+VP MILGETALSFLGLGLRPP+TSWGVLLNEAQN
Sbjct:  171 LGRDLLSRIIYGTRISLTVGLFGIMVSFTIGIVLGGISGYYGGWIDNLIQRFIEMVRAFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARAVRSKLLALREEDFAAAAVLMGAGPGRVIARHLLPSFASHLIASATLAVPGMILGETALSFLGLGLRPPITSWGVLLNEAQN 351
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: A0A1F3YU29_9PROT (Peptide ABC transporter permease n=1 Tax=Betaproteobacteria bacterium RBG_16_66_20 TaxID=1797479 RepID=A0A1F3YU29_9PROT)

HSP 1 Score: 320 bits (821), Expect = 3.480e-107
Identity = 164/181 (90.61%), Postives = 175/181 (96.69%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRD+LSRI++GTRISLTVGLLGI VSFLIGI LGGISGY+GGWID++IQRFIEMVRSFPELPLWMALSAALPV WSP+LVYFGITIILGLLDWPGLAR+VRSKLLALREE+FA AAVLMGA+P RVI RHLLPSFASHLIAS+TLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN
Sbjct:  165 LGRDVLSRIIHGTRISLTVGLLGIAVSFLIGIILGGISGYYGGWIDSLIQRFIEMVRSFPELPLWMALSAALPVNWSPVLVYFGITIILGLLDWPGLARAVRSKLLALREEEFATAAVLMGATPGRVIGRHLLPSFASHLIASLTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 345
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: A0A516H6R0_9PROT (ABC transporter permease n=3 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A516H6R0_9PROT)

HSP 1 Score: 315 bits (808), Expect = 4.070e-105
Identity = 158/181 (87.29%), Postives = 170/181 (93.92%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRD+ SR+VYGTRISLTVGL GI +SF+IGI LGGISGY+GGWIDNVIQR IE++RS PELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLAR+VRSKLLALREEDFA AA LMGASP+R+I RHLLPSF SHLIAS TLS+PSMILGETALSFLGLGLRPP+TSWGVLLNEAQN
Sbjct:  172 LGRDIFSRVVYGTRISLTVGLFGIAISFIIGIVLGGISGYYGGWIDNVIQRLIELIRSLPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARAVRSKLLALREEDFATAAQLMGASPSRIIGRHLLPSFMSHLIASATLSIPSMILGETALSFLGLGLRPPITSWGVLLNEAQN 352
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: A0A1G3INL2_9PROT (Peptide ABC transporter permease n=1 Tax=Rhodospirillales bacterium RIFCSPLOWO2_12_FULL_67_15 TaxID=1802023 RepID=A0A1G3INL2_9PROT)

HSP 1 Score: 313 bits (801), Expect = 5.150e-104
Identity = 163/181 (90.06%), Postives = 167/181 (92.27%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRDL SRIVYGTRISLTVGLLGI+VSF IGITLGGISGYFGG ID +IQRFIEMV SFPELPLWMALSAALPV WSPILVYFGITIILGLLDWPGLAR+VRSKLLALREEDF  AAVLMGA P RVI RHLLPSF SHLIAS TLSVP MI+GETALSFLGLGLRPPVTSWGVLLNEAQN
Sbjct:  171 LGRDLFSRIVYGTRISLTVGLLGIIVSFTIGITLGGISGYFGGRIDTLIQRFIEMVSSFPELPLWMALSAALPVNWSPILVYFGITIILGLLDWPGLARAVRSKLLALREEDFVTAAVLMGAGPGRVIGRHLLPSFTSHLIASATLSVPGMIMGETALSFLGLGLRPPVTSWGVLLNEAQN 351
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: A0A7S8HCG7_9HYPH (ABC transporter permease n=1 Tax=Kaustia mangrovi TaxID=2593653 RepID=A0A7S8HCG7_9HYPH)

HSP 1 Score: 312 bits (799), Expect = 1.000e-103
Identity = 154/181 (85.08%), Postives = 172/181 (95.03%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRD+LSRIVYGTRISLTVGL+GI +SFLIGITLGG++GY+GGW+D+++QR IE++RS PELPLW+ALSAALPVTWSPIL+YFGITIILGLLDWPGLAR+VRSKLLALREEDFA AAVLMGA P RVI RHLLP+F SHLIAS TLSVP+MILGETALSFLGLGLRPP+TSWGVLLNEAQN
Sbjct:  174 LGRDMLSRIVYGTRISLTVGLIGITISFLIGITLGGLAGYYGGWVDSIVQRMIEILRSLPELPLWLALSAALPVTWSPILIYFGITIILGLLDWPGLARAVRSKLLALREEDFATAAVLMGAKPGRVIGRHLLPNFMSHLIASATLSVPAMILGETALSFLGLGLRPPITSWGVLLNEAQN 354
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: A0A3B0T6U0_9ZZZZ (ABC transporter, permease protein 2 (Cluster 5, nickel/peptides/opines) (Fragment) n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B0T6U0_9ZZZZ)

HSP 1 Score: 307 bits (787), Expect = 3.630e-102
Identity = 154/181 (85.08%), Postives = 167/181 (92.27%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRD+ SRI YGTRISLTVGLLGI +SFL GI LGG+SGY+GGWIDNVIQR IE++RS PELPLW+ALSAALPVTWSPILV+ GIT+ILGLLDWPGLAR+VRSKLLALREEDFA AAVLMGA P RVI RHLLPSFASHLIAS +LS+P MILGETALSFLGLGLRPP+TSWGVLLNEAQN
Sbjct:  179 LGRDMFSRITYGTRISLTVGLLGITISFLFGIILGGLSGYYGGWIDNVIQRVIEIIRSLPELPLWLALSAALPVTWSPILVFLGITVILGLLDWPGLARAVRSKLLALREEDFATAAVLMGAKPKRVIARHLLPSFASHLIASASLSIPGMILGETALSFLGLGLRPPITSWGVLLNEAQN 359
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BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Match: UPI001FE554EF (ABC transporter permease n=1 Tax=Azospirillum oleiclasticum TaxID=2735135 RepID=UPI001FE554EF)

HSP 1 Score: 306 bits (785), Expect = 3.850e-102
Identity = 153/181 (84.53%), Postives = 168/181 (92.82%), Query Frame = 1 
Query:    1 LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARSVRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPSMILGETALSFLGLGLRPPVTSWGVLLNEAQN 543
            LGRD+LSRIVYGTRISLTVGLLGI+VSF +GI +GGI+GY+GGWIDN+IQRFIE+VRSFPELPLWMALSAALPVTW+PI VYFGIT+ILGLLDW GLAR+VRSKLLALREEDF  AA LMGA P+R+I RHLLPSF SHLIAS TLS+P MILGETALSFLGLGLRPP+TSWGVLL EAQN
Sbjct:  135 LGRDVLSRIVYGTRISLTVGLLGIIVSFTLGIVIGGIAGYYGGWIDNIIQRFIEIVRSFPELPLWMALSAALPVTWNPIFVYFGITLILGLLDWTGLARAVRSKLLALREEDFTTAAQLMGARPSRIIGRHLLPSFMSHLIASATLSIPGMILGETALSFLGLGLRPPITSWGVLLTEAQN 315
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig52707.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1F4F1T5_9PROT1.530e-10992.82Peptide ABC transporter permease n=5 Tax=unclassif... [more]
A0A536WL82_9PROT4.360e-10991.71ABC transporter permease n=1 Tax=Betaproteobacteri... [more]
UPI0019B9F0625.190e-10891.16ABC transporter permease n=1 Tax=Rhodoferax sp. Ta... [more]
A0A0S8B4T1_9PROT1.040e-10790.61Peptide ABC transporter permease n=1 Tax=Betaprote... [more]
A0A1F3YU29_9PROT3.480e-10790.61Peptide ABC transporter permease n=1 Tax=Betaprote... [more]
A0A516H6R0_9PROT4.070e-10587.29ABC transporter permease n=3 Tax=Alphaproteobacter... [more]
A0A1G3INL2_9PROT5.150e-10490.06Peptide ABC transporter permease n=1 Tax=Rhodospir... [more]
A0A7S8HCG7_9HYPH1.000e-10385.08ABC transporter permease n=1 Tax=Kaustia mangrovi ... [more]
A0A3B0T6U0_9ZZZZ3.630e-10285.08ABC transporter, permease protein 2 (Cluster 5, ni... [more]
UPI001FE554EF3.850e-10284.53ABC transporter permease n=1 Tax=Azospirillum olei... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig52707contigP-wetherbeei_contig52707:2..544 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1121033.AUCF01000003_gene3332
PFAMsBPD_transp_1,OppC_N
Model size543
Max annot lvl204441|Rhodospirillales
KEGG koko:K02034
KEGG TC3.A.1.5
KEGG Pathwayko02024,map02024
KEGG ModuleM00239
Hectar predicted targeting categorysignal anchor
Exons1
Evalue5.37e-101
EggNOG OGsCOG1173@1|root,COG1173@2|Bacteria,1MU26@1224|Proteobacteria,2TUWG@28211|Alphaproteobacteria,2JQ92@204441|Rhodospirillales
DescriptionN-terminal TM domain of oligopeptide transport permease C
Cds size543
COG categoryEP
BRITEko00000,ko00001,ko00002,ko02000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186789.8616598-CDS-P-wetherbeei_contig52707:1..5441683186789.8616598-CDS-P-wetherbeei_contig52707:1..544Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig52707 2..544 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig52707.1.1prot_P-wetherbeei_contig52707.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig52707 2..544 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig52707.1.1

>prot_P-wetherbeei_contig52707.1.1 ID=prot_P-wetherbeei_contig52707.1.1|Name=mRNA_P-wetherbeei_contig52707.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=181bp
LGRDLLSRIVYGTRISLTVGLLGILVSFLIGITLGGISGYFGGWIDNVIQ
RFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARS
VRSKLLALREEDFANAAVLMGASPARVIRRHLLPSFASHLIASVTLSVPS
MILGETALSFLGLGLRPPVTSWGVLLNEAQN
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mRNA from alignment at P-wetherbeei_contig52707:2..544+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig52707.1.1 ID=mRNA_P-wetherbeei_contig52707.1.1|Name=mRNA_P-wetherbeei_contig52707.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=543bp|location=Sequence derived from alignment at P-wetherbeei_contig52707:2..544+ (Phaeothamnion wetherbeei SAG_119_79)
CTCGGCCGCGACCTTCTGTCACGCATCGTGTACGGGACCCGGATTTCCCT
GACAGTAGGCCTGCTCGGGATCCTCGTGAGCTTCCTGATAGGCATCACGC
TGGGCGGTATTTCCGGATACTTCGGTGGATGGATAGATAACGTTATCCAG
CGCTTCATCGAGATGGTGAGGTCCTTCCCGGAGTTGCCCCTGTGGATGGC
GCTGTCGGCCGCCTTGCCGGTCACCTGGAGCCCGATCCTGGTGTACTTCG
GCATAACGATCATCCTCGGGCTTCTCGACTGGCCGGGCCTCGCGAGGTCG
GTGCGCTCCAAGCTGCTGGCACTCAGGGAGGAGGATTTCGCCAACGCCGC
CGTGCTGATGGGAGCGAGTCCCGCGCGGGTGATCCGGCGCCACCTTCTGC
CGAGCTTCGCGAGCCATCTCATCGCTTCGGTCACGCTCTCCGTGCCCAGC
ATGATCCTGGGCGAGACCGCGCTGTCCTTCCTCGGGCTCGGGCTGCGCCC
GCCCGTAACCAGCTGGGGCGTGCTGCTGAACGAGGCGCAGAAC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig52707:2..544+

>mRNA_P-wetherbeei_contig52707.1.1 ID=mRNA_P-wetherbeei_contig52707.1.1|Name=mRNA_P-wetherbeei_contig52707.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=543bp|location=Sequence derived from alignment at P-wetherbeei_contig52707:2..544+ (Phaeothamnion wetherbeei SAG_119_79)
CTCGGCCGCGACCTTCTGTCACGCATCGTGTACGGGACCCGGATTTCCCT
GACAGTAGGCCTGCTCGGGATCCTCGTGAGCTTCCTGATAGGCATCACGC
TGGGCGGTATTTCCGGATACTTCGGTGGATGGATAGATAACGTTATCCAG
CGCTTCATCGAGATGGTGAGGTCCTTCCCGGAGTTGCCCCTGTGGATGGC
GCTGTCGGCCGCCTTGCCGGTCACCTGGAGCCCGATCCTGGTGTACTTCG
GCATAACGATCATCCTCGGGCTTCTCGACTGGCCGGGCCTCGCGAGGTCG
GTGCGCTCCAAGCTGCTGGCACTCAGGGAGGAGGATTTCGCCAACGCCGC
CGTGCTGATGGGAGCGAGTCCCGCGCGGGTGATCCGGCGCCACCTTCTGC
CGAGCTTCGCGAGCCATCTCATCGCTTCGGTCACGCTCTCCGTGCCCAGC
ATGATCCTGGGCGAGACCGCGCTGTCCTTCCTCGGGCTCGGGCTGCGCCC
GCCCGTAACCAGCTGGGGCGTGCTGCTGAACGAGGCGCAGAAC
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