mRNA_P-wetherbeei_contig52404.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig52404.1.1
Unique NamemRNA_P-wetherbeei_contig52404.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: A0A3A9JIJ4_9PROT (Thiolase family protein n=3 Tax=Roseomonas TaxID=125216 RepID=A0A3A9JIJ4_9PROT)

HSP 1 Score: 252 bits (644), Expect = 2.490e-80
Identity = 114/181 (62.98%), Postives = 146/181 (80.66%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            R+   QYGG QAMGGD +LSYVYH+PWGLTSQGALYA+  + + + RG+T+ +LGEVAVA+R W+ +NP AIM+K +TL+DY+AS YVCEPLHLFDYC++NDGGVA+IIAEA RA  MCERPV+IE +GR +LNRGAT+LE R+  FY  AQ + A++ +   G GPED+ + Q+YDSFSV
Sbjct:  118 RTAGTQYGGPQAMGGDAFLSYVYHSPWGLTSQGALYALTFQAYKQARGFTEAELGEVAVAQRGWSSMNPNAIMRKRITLEDYMASNYVCEPLHLFDYCLINDGGVAMIIAEAERAKRMCERPVFIEAVGRSDLNRGATALEPRITEFYLPAQQQAAARSYEVAGVGPEDMDVFQVYDSFSV 298
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: A0A1M5R3W8_9BURK (Acetyl-CoA acetyltransferase n=1 Tax=Candidimonas bauzanensis TaxID=658167 RepID=A0A1M5R3W8_9BURK)

HSP 1 Score: 248 bits (632), Expect = 1.920e-78
Identity = 114/181 (62.98%), Postives = 143/181 (79.01%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            RS ++QYGG QAMGGD +LSYVYHAPWGLTSQGALYA+  R + E RG+   DLG+VAVA+RAW+ +NP A+M+K +TLDDY  + Y+CEPL L+DYCM+NDGGVALII  A RAA + +  +YIE +GR +LNRGATSLE RL +FY  AQ + A Q+++  GFGPED+ L Q+YDSFSV
Sbjct:  124 RSAKIQYGGPQAMGGDAFLSYVYHAPWGLTSQGALYALTFRAYCEERGFDPLDLGQVAVAQRAWSSMNPNAVMRKRITLDDYRQAQYICEPLRLYDYCMINDGGVALIITTAERAARLAKPAIYIEAVGRRDLNRGATSLEPRLTDFYLPAQQDCARQVYDMAGFGPEDMDLFQVYDSFSV 304
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: A0A0P0RM29_9BURK (Putative thiolase n=4 Tax=Burkholderiaceae TaxID=119060 RepID=A0A0P0RM29_9BURK)

HSP 1 Score: 247 bits (631), Expect = 2.640e-78
Identity = 114/181 (62.98%), Postives = 140/181 (77.35%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            RS ++QYGG QAMGGD +LSYVYHAPWGLTSQGALYA+  R F E RG+   DLG VAVA+RAW+ +NP A+M+K +T D+Y AS ++CEPL LFDYCM+NDGGVALI+  A RA+   + PVYIE +GR +LNRGATSLE RL +FY  AQ + A Q ++  GFGP D+ L Q+YDSFSV
Sbjct:  123 RSAQIQYGGPQAMGGDAFLSYVYHAPWGLTSQGALYALTFRAFCEARGFDPLDLGHVAVAQRAWSSMNPNAVMRKTITFDEYCASRHICEPLRLFDYCMINDGGVALIVTTAERASRFAKPPVYIEALGRRDLNRGATSLEPRLTDFYLPAQQDCARQAYDMAGFGPTDIDLFQVYDSFSV 303
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: A0A2W7IRF8_9PROT (Acetyl-CoA acetyltransferase n=1 Tax=Humitalea rosea TaxID=990373 RepID=A0A2W7IRF8_9PROT)

HSP 1 Score: 247 bits (630), Expect = 3.420e-78
Identity = 113/181 (62.43%), Postives = 145/181 (80.11%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            R+   QYGG QAMGGD +LSYVYHAPWGLTSQGALYA+  + +   RG T+ +LGEVAVA+R W+ +NP AIM+K +T++DY+AS YVCEPLHLFDYC++NDGGVA+IIAEA  A  +CE PV+I+ +GR +LNRGATSLE RL +FY  AQ + A++ +   GFGPED+ ++Q+YDSFSV
Sbjct:  120 RTAGTQYGGPQAMGGDAFLSYVYHAPWGLTSQGALYALTFQAYKNARGMTEAELGEVAVAQRGWSSMNPNAIMRKRITVEDYMASRYVCEPLHLFDYCLINDGGVAMIIAEAEYAKRICETPVFIQGIGRSDLNRGATSLEPRLTDFYLPAQQQAAARSYAMAGFGPEDIDVLQVYDSFSV 300
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: A0A7L5C081_9RHOB (Thiolase family protein n=1 Tax=Pikeienuella piscinae TaxID=2748098 RepID=A0A7L5C081_9RHOB)

HSP 1 Score: 247 bits (630), Expect = 3.730e-78
Identity = 114/181 (62.98%), Postives = 142/181 (78.45%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            RS + QYGG  AMGGD +LSYVYH+PWGLTSQGALYA+  + +   RG+T+ +LGEVAVA+R W+ LNP AIM+K +T++DYLASPY+CEPLHLFDYC++NDGGVALIIAEA  A  M ERPV+IE +GR +LN+ ATSLE RL +FY  AQ +VA ++F+     P DV   Q+YDSFSV
Sbjct:  123 RSAQTQYGGPNAMGGDAFLSYVYHSPWGLTSQGALYALTLQAYKHARGFTEAELGEVAVAQRGWSSLNPNAIMRKRITVEDYLASPYICEPLHLFDYCLINDGGVALIIAEAELAKKMTERPVFIEAVGRRDLNQAATSLEPRLTDFYLPAQQDVAERVFSTANIEPRDVDAFQVYDSFSV 303
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: A0A3N1M9S4_9PROT (Acetyl-CoA acetyltransferase n=1 Tax=Stella humosa TaxID=94 RepID=A0A3N1M9S4_9PROT)

HSP 1 Score: 245 bits (625), Expect = 2.970e-77
Identity = 115/181 (63.54%), Postives = 141/181 (77.90%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            RS   QYGG  AMGG+ +LSYVYHAPWGLTSQGALYA+  + +    G+T+RDLGEVAVA+R W+ LNP AIM++ +T+DDY+AS YVCEPLHLFDYC++NDGGVALIIAEA  A  +  +PVYIE  GR +LN GATSLE RL +FY  AQ  VA Q+F+  G GP+D+ + Q+YDSFSV
Sbjct:  135 RSVGTQYGGPDAMGGNAFLSYVYHAPWGLTSQGALYALTLQAYKHAHGFTERDLGEVAVAQRGWSSLNPNAIMRERITVDDYMASRYVCEPLHLFDYCLINDGGVALIIAEADIARRISSKPVYIEAAGRSDLNHGATSLEPRLTDFYLPAQQAVAQQVFDMAGMGPKDMDVFQVYDSFSV 315
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: UPI001BAC89C6 (thiolase family protein n=2 Tax=Roseomonas hellenica TaxID=2687306 RepID=UPI001BAC89C6)

HSP 1 Score: 244 bits (623), Expect = 4.750e-77
Identity = 112/181 (61.88%), Postives = 146/181 (80.66%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            RS   QYGG  AMGG+ +LSYVYH+PWG TSQGALYA+  + F   RG+T+ ++GEV+VA+R+W+ LNP AIM+K +TL+DYLAS +VCEPLHLFDYC++NDGGVALIIAEA RA  M +RPV+IE +GR +LNRGATSLE R+++FY  AQ   A+Q +  +G GP+D+ ++Q+YDSFSV
Sbjct:  127 RSVGTQYGGPDAMGGNAFLSYVYHSPWGFTSQGALYALTLQSFKHARGFTEAEMGEVSVAQRSWSALNPNAIMRKRITLEDYLASHFVCEPLHLFDYCLINDGGVALIIAEAERAKRMTDRPVFIEGVGRFDLNRGATSLEPRIDDFYLPAQQSAAAQSYAVSGLGPKDMDILQVYDSFSV 307
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: A0A158F6H5_9BURK (Thiolase family protein n=2 Tax=Caballeronia TaxID=1827195 RepID=A0A158F6H5_9BURK)

HSP 1 Score: 243 bits (620), Expect = 1.200e-76
Identity = 112/181 (61.88%), Postives = 140/181 (77.35%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            RS E+QYGG QAMGGD +LSYVYH+PWGLTSQGALYA+  R +   RG+   DLG+VAVA+RAW+ +NP A+M+K +TLDDY  + Y+CEPL L+DYCM+NDGGVALI+  A RA  + + PVYIE +GR +LNRGATSLE RL +FY  AQ + A Q ++  GFGP D+ L Q+YDSFSV
Sbjct:  123 RSAEIQYGGPQAMGGDAFLSYVYHSPWGLTSQGALYALTFRAYCHERGFDPLDLGQVAVAQRAWSSMNPNAVMRKRITLDDYREAKYICEPLRLYDYCMINDGGVALIVTTAERAQKLRKPPVYIEALGRRDLNRGATSLEPRLTDFYLSAQQDCARQAYDMAGFGPRDMDLFQVYDSFSV 303
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: A0A7Y0HBN4_9BURK (Thiolase family protein n=1 Tax=Glaciimonas sp. TaxID=2021635 RepID=A0A7Y0HBN4_9BURK)

HSP 1 Score: 243 bits (619), Expect = 1.600e-76
Identity = 118/180 (65.56%), Postives = 140/180 (77.78%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFS 540
            RS  + YGGA A  G  ++SYVYHAPWGLTSQGALYA++ RR+ E  G T+RDL EVAVA+R  A LN  AIM+KP+T+DDYL SPYVCEPLHLFDYC+VNDGGVALII +A RA  M + PV I  +GR++LNRGATSLE RL NFY  AQ + A Q+FN  G GPEDV+ +Q+YDSFS
Sbjct:  121 RSAGLNYGGANAAFGSTFMSYVYHAPWGLTSQGALYALMFRRYMEETGLTERDLAEVAVAQRFAASLNDNAIMRKPITVDDYLQSPYVCEPLHLFDYCLVNDGGVALIITDARRAKKMRKDPVVIHGVGRYDLNRGATSLEPRLTNFYLPAQQKAAHQVFNMAGIGPEDVNSLQVYDSFS 300
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BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Match: UPI001F19F705 (thiolase family protein n=1 Tax=Acuticoccus sp. M5D2P5 TaxID=2910977 RepID=UPI001F19F705)

HSP 1 Score: 242 bits (618), Expect = 1.910e-76
Identity = 117/182 (64.29%), Postives = 144/182 (79.12%), Query Frame = 1 
Query:    1 RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYTDRDLGEVAVAERAWAGLNPAAIMQ-KPVTLDDYLASPYVCEPLHLFDYCMVNDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYADAQAEVASQIFNATGFGPEDVSLVQIYDSFSV 543
            RS   QYGG QAMGGD +LSYVYHAPWGLTSQGALYA++ +RF  + G+ + DLG VAVA+RA A  NP AIMQ +P++L+DYL S Y+CEPLHLFDYC++NDGGVALIIAEA RA  + +RPVYI+ +GR++LN  AT+LE RL  FY  AQ +VA Q+FN  G GP+DV + Q+YDSFSV
Sbjct:  114 RSVGTQYGGPQAMGGDAFLSYVYHAPWGLTSQGALYALMFQRFRALYGFEESDLGHVAVAQRASAQHNPNAIMQGRPISLEDYLGSRYICEPLHLFDYCLINDGGVALIIAEAERARAITDRPVYIDAVGRYDLNENATTLEPRLTEFYRPAQQKVAEQVFNRAGIGPDDVDVFQVYDSFSV 295
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig52404.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3A9JIJ4_9PROT2.490e-8062.98Thiolase family protein n=3 Tax=Roseomonas TaxID=1... [more]
A0A1M5R3W8_9BURK1.920e-7862.98Acetyl-CoA acetyltransferase n=1 Tax=Candidimonas ... [more]
A0A0P0RM29_9BURK2.640e-7862.98Putative thiolase n=4 Tax=Burkholderiaceae TaxID=1... [more]
A0A2W7IRF8_9PROT3.420e-7862.43Acetyl-CoA acetyltransferase n=1 Tax=Humitalea ros... [more]
A0A7L5C081_9RHOB3.730e-7862.98Thiolase family protein n=1 Tax=Pikeienuella pisci... [more]
A0A3N1M9S4_9PROT2.970e-7763.54Acetyl-CoA acetyltransferase n=1 Tax=Stella humosa... [more]
UPI001BAC89C64.750e-7761.88thiolase family protein n=2 Tax=Roseomonas helleni... [more]
A0A158F6H5_9BURK1.200e-7661.88Thiolase family protein n=2 Tax=Caballeronia TaxID... [more]
A0A7Y0HBN4_9BURK1.600e-7665.56Thiolase family protein n=1 Tax=Glaciimonas sp. Ta... [more]
UPI001F19F7051.910e-7664.29thiolase family protein n=1 Tax=Acuticoccus sp. M5... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig52404contigP-wetherbeei_contig52404:3..545 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1097668.BYI23_C006070
Model size543
Max annot lvl28216|Betaproteobacteria
Hectar predicted targeting categoryother localisation
Exons1
Evalue3.47e-78
EggNOG OGsCOG0183@1|root,COG0183@2|Bacteria,1MUZV@1224|Proteobacteria,2VMWG@28216|Betaproteobacteria,1K4A2@119060|Burkholderiaceae
DescriptionCatalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation
Cds size543
COG categoryI
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186785.3538547-CDS-P-wetherbeei_contig52404:2..5451683186785.3538547-CDS-P-wetherbeei_contig52404:2..545Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig52404 3..545 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig52404.1.1prot_P-wetherbeei_contig52404.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig52404 3..545 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig52404.1.1

>prot_P-wetherbeei_contig52404.1.1 ID=prot_P-wetherbeei_contig52404.1.1|Name=mRNA_P-wetherbeei_contig52404.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=181bp
RSGEVQYGGAQAMGGDNYLSYVYHAPWGLTSQGALYAMLQRRFAEVRGYT
DRDLGEVAVAERAWAGLNPAAIMQKPVTLDDYLASPYVCEPLHLFDYCMV
NDGGVALIIAEAGRAAHMCERPVYIETMGRHELNRGATSLESRLENFYAD
AQAEVASQIFNATGFGPEDVSLVQIYDSFSV
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mRNA from alignment at P-wetherbeei_contig52404:3..545-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig52404.1.1 ID=mRNA_P-wetherbeei_contig52404.1.1|Name=mRNA_P-wetherbeei_contig52404.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=543bp|location=Sequence derived from alignment at P-wetherbeei_contig52404:3..545- (Phaeothamnion wetherbeei SAG_119_79)
CGTAGCGGCGAGGTGCAGTACGGCGGCGCCCAGGCAATGGGGGGAGACAA
CTACCTTTCCTACGTGTATCACGCGCCGTGGGGGCTTACATCGCAGGGGG
CGCTGTACGCCATGCTGCAGCGGCGTTTCGCCGAGGTGCGCGGCTACACC
GACCGCGACCTGGGCGAGGTGGCGGTTGCCGAGCGCGCCTGGGCGGGGCT
CAATCCGGCAGCCATCATGCAAAAGCCCGTCACGCTCGACGACTACCTCG
CCTCGCCCTACGTGTGCGAGCCGCTGCACCTGTTCGACTACTGCATGGTC
AACGACGGCGGCGTGGCGCTCATCATCGCCGAGGCCGGGCGGGCGGCGCA
CATGTGCGAGCGCCCGGTGTACATCGAGACCATGGGCCGCCATGAACTCA
ACCGCGGCGCGACCTCGCTGGAGTCGCGGCTGGAGAACTTCTATGCCGAT
GCCCAGGCCGAAGTGGCGTCGCAGATCTTCAATGCCACCGGCTTCGGCCC
GGAGGATGTGAGCCTGGTGCAGATCTACGACAGCTTCTCGGTG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig52404:3..545-

>mRNA_P-wetherbeei_contig52404.1.1 ID=mRNA_P-wetherbeei_contig52404.1.1|Name=mRNA_P-wetherbeei_contig52404.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=543bp|location=Sequence derived from alignment at P-wetherbeei_contig52404:3..545- (Phaeothamnion wetherbeei SAG_119_79)
CGTAGCGGCGAGGTGCAGTACGGCGGCGCCCAGGCAATGGGGGGAGACAA
CTACCTTTCCTACGTGTATCACGCGCCGTGGGGGCTTACATCGCAGGGGG
CGCTGTACGCCATGCTGCAGCGGCGTTTCGCCGAGGTGCGCGGCTACACC
GACCGCGACCTGGGCGAGGTGGCGGTTGCCGAGCGCGCCTGGGCGGGGCT
CAATCCGGCAGCCATCATGCAAAAGCCCGTCACGCTCGACGACTACCTCG
CCTCGCCCTACGTGTGCGAGCCGCTGCACCTGTTCGACTACTGCATGGTC
AACGACGGCGGCGTGGCGCTCATCATCGCCGAGGCCGGGCGGGCGGCGCA
CATGTGCGAGCGCCCGGTGTACATCGAGACCATGGGCCGCCATGAACTCA
ACCGCGGCGCGACCTCGCTGGAGTCGCGGCTGGAGAACTTCTATGCCGAT
GCCCAGGCCGAAGTGGCGTCGCAGATCTTCAATGCCACCGGCTTCGGCCC
GGAGGATGTGAGCCTGGTGCAGATCTACGACAGCTTCTCGGTG
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