mRNA_P-wetherbeei_contig52136.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig52136.1.1
Unique NamemRNA_P-wetherbeei_contig52136.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: A0A7L9BHV4_9BACT (M20/M25/M40 family metallo-hydrolase n=1 Tax=Flammeovirgaceae bacterium TaxID=2026740 RepID=A0A7L9BHV4_9BACT)

HSP 1 Score: 297 bits (760), Expect = 8.370e-96
Identity = 139/182 (76.37%), Postives = 162/182 (89.01%), Query Frame = 1 
Query:    1 VSFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            +S S+S K  +D SYN+V KITG  QP EY++YSTHWDHLGIGKAD+ GDTIYNGALDNASG+ G+LE+AKAF S+KNKP+R++VFL+VTAEEQ LLGS++YA NPIYP EKTVANINIDG+NPYGK  DIV+VG+GQSDLEDYL+EEAK VG Y +PEPNPVAGYYFRSDHFSFAKVG+PA
Sbjct:  285 LSTSLSVKATFDTSYNVVGKITGAQQPGEYIIYSTHWDHLGIGKADERGDTIYNGALDNASGTAGMLELAKAFKSVKNKPSRSIVFLFVTAEEQNLLGSAHYAANPIYPVEKTVANINIDGINPYGKMNDIVVVGIGQSDLEDYLNEEAKAVGRYTSPEPNPVAGYYFRSDHFSFAKVGVPA 466
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: UPI001FA7870B (M28 family metallopeptidase n=1 Tax=Pontibacter sp. NBU2971 TaxID=2905677 RepID=UPI001FA7870B)

HSP 1 Score: 270 bits (691), Expect = 2.210e-85
Identity = 126/182 (69.23%), Postives = 154/182 (84.62%), Query Frame = 1 
Query:    1 VSFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            VS +M+ K KYDKS+N +AK+TG   PDE +LYS HWDHLGIGKAD TGD+IYNGA+D+ASG  G+LEIAK F S   +P R++VFL VTAEEQGL GS+YYA++P++PKEKTVANIN+D +N YG+ KDIVL GMGQS+LEDY++EE K+VG YVAPEPNP AG Y+RSDHF+FAK+GIPA
Sbjct:  295 VSTAMTVKTKYDKSHNFIAKLTGAKSPDENILYSAHWDHLGIGKADATGDSIYNGAVDDASGVAGLLEIAKVFKSQPGRPERSIVFLAVTAEEQGLWGSAYYAEHPVFPKEKTVANINMDMLNSYGRTKDIVLFGMGQSELEDYIAEEGKKVGRYVAPEPNPEAGLYYRSDHFNFAKIGIPA 476
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: UPI001FA75204 (M28 family metallopeptidase n=1 Tax=Pontibacter sp. NBU2972 TaxID=2905679 RepID=UPI001FA75204)

HSP 1 Score: 270 bits (691), Expect = 2.510e-85
Identity = 129/182 (70.88%), Postives = 153/182 (84.07%), Query Frame = 1 
Query:    1 VSFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            VS SM  K KYDKS+N +AKITG   P+E ++YS HWDHLG GKAD TGD+IYNGA+D+ASG  G+LEIAKAF SL  KP R++VFL VTAEEQGL GS+YYAQNP++PKEK+VANIN+D +N YG+ KDIVL GMGQS+LEDYL+EE K+VG YVA EPNP AG Y+RSDHF+FAK+GIPA
Sbjct:  300 VSTSMMVKTKYDKSHNFIAKITGTKNPEENIIYSAHWDHLGYGKADATGDSIYNGAVDDASGVAGLLEIAKAFKSLPVKPERSIVFLAVTAEEQGLWGSAYYAQNPVFPKEKSVANINMDMLNSYGRTKDIVLFGMGQSELEDYLAEEGKKVGRYVAAEPNPEAGLYYRSDHFNFAKIGIPA 481
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: A0A1N6TXX0_9BACT (Zn-dependent amino-or carboxypeptidase, M28 family n=5 Tax=Pontibacter TaxID=323449 RepID=A0A1N6TXX0_9BACT)

HSP 1 Score: 267 bits (682), Expect = 4.670e-84
Identity = 124/181 (68.51%), Postives = 152/181 (83.98%), Query Frame = 1 
Query:    4 SFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            S S+  K +YDK+ N +AKITG T+PDE ++Y+ HWDHLGIGKAD TGD+IYNGA+DNASG  G+LEIAKAF +   +P RTVVFL VTAEEQGL GS+YYA+NP++PKEKTVANIN+D +NPYGK KD+VL GMGQS+LEDY++ EA+  G Y+APE NP AG Y+RSDHF+FAK+GIPA
Sbjct:  293 STSLKVKTRYDKTSNFIAKITGSTRPDETIIYTGHWDHLGIGKADATGDSIYNGAVDNASGVAGLLEIAKAFKNQPTQPERTVVFLAVTAEEQGLWGSAYYAENPVFPKEKTVANINMDMMNPYGKTKDVVLYGMGQSELEDYITAEAEAAGRYIAPEENPEAGLYYRSDHFNFAKIGIPA 473
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: UPI00117D7624 (M28 family metallopeptidase n=1 Tax=Litoribacter populi TaxID=2598460 RepID=UPI00117D7624)

HSP 1 Score: 266 bits (681), Expect = 5.680e-84
Identity = 124/181 (68.51%), Postives = 149/181 (82.32%), Query Frame = 1 
Query:    4 SFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            S SM    +YD S N++AK+ G  +PDEY++Y+ HWDHLGIGK D+TGD+IYNGALDNASG+  +LEIA+AF     KP RTVVFL VTAEEQGL GS+YYA+NP++PKEKTVANIN+DG+NPYGK KDIVLVG GQS+LE  L EEA++V  Y +PEP P AGYYFRSDHF+FAK+GIPA
Sbjct:  287 SSSMEVSAEYDVSQNVIAKVEGSKRPDEYIIYTAHWDHLGIGKPDETGDSIYNGALDNASGTAALLEIARAFQEANEKPERTVVFLAVTAEEQGLWGSAYYAENPVFPKEKTVANINMDGINPYGKMKDIVLVGKGQSELELLLEEEAEKVDRYTSPEPTPSAGYYFRSDHFNFAKIGIPA 467
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: A0A074KXV5_9BACT (Peptidase M28 n=1 Tax=Anditalea andensis TaxID=1048983 RepID=A0A074KXV5_9BACT)

HSP 1 Score: 266 bits (681), Expect = 5.820e-84
Identity = 123/173 (71.10%), Postives = 147/173 (84.97%), Query Frame = 1 
Query:   28 KYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            KY+ S N +A I G  +PDEY++Y+ HWDHLGIGK D+TGD+IYNGALDNASG+  +LE+A+AF   +N+P RT+VFL VTAEEQGL GS+YYA NPIYPKEKTVANIN+DG+NPYGK KDIVLVG GQS+LE+ L EEAK+VG Y +PEP P AGYYFRSDHF+FAK+GIPA
Sbjct:  293 KYNVSKNAIAMIEGSKRPDEYIIYTAHWDHLGIGKPDETGDSIYNGALDNASGTAALLELARAFNQSENRPERTIVFLAVTAEEQGLWGSAYYAANPIYPKEKTVANINMDGINPYGKMKDIVLVGKGQSELEEILEEEAKKVGRYTSPEPTPSAGYYFRSDHFNFAKIGIPA 465
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: A0A142ELU3_9BACT (Peptidase M28 n=1 Tax=Algoriphagus sanaruensis TaxID=1727163 RepID=A0A142ELU3_9BACT)

HSP 1 Score: 266 bits (680), Expect = 6.890e-84
Identity = 123/182 (67.58%), Postives = 155/182 (85.16%), Query Frame = 1 
Query:    1 VSFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            +S SM+ K  Y+ S N+VAK+TGK++P+EY++YS HWDH GIGK D+TGD+IYNGALDNASG+  ++ +AKAF +    P RTVVFL VTAEEQGL GS+YYAQNPIYPKEKTVANIN+DG+NPYGK KD+ ++G+GQS++ED L+ E +++G Y APEPNPVAGYYFRSDHF+FAK+GIPA
Sbjct:  280 LSVSMNVKATYNSSKNVVAKLTGKSRPEEYIIYSAHWDHFGIGKPDETGDSIYNGALDNASGTAALIALAKAFKT-DGAPDRTVVFLSVTAEEQGLWGSAYYAQNPIYPKEKTVANINMDGINPYGKMKDVSIIGVGQSEMEDLLNVELEKLGRYSAPEPNPVAGYYFRSDHFNFAKIGIPA 460
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: UPI001BD20998 (M28 family metallopeptidase n=2 Tax=Litoribacter TaxID=1237104 RepID=UPI001BD20998)

HSP 1 Score: 266 bits (680), Expect = 8.010e-84
Identity = 123/181 (67.96%), Postives = 151/181 (83.43%), Query Frame = 1 
Query:    4 SFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            S S+    +YD S N++AK+ G  + DEY++Y+ HWDHLGIGK D+TGD+IYNGALDNASG+  +LEIA+AF     KP RTVVFL VTAEEQGL GS+YYA+NP++PKEKTVANIN+DG+NPYGK KDIVLVGMGQS+LE+ L++EA +VG Y +PEP P AGYYFRSDHF+FAK+GIPA
Sbjct:  287 SSSLEVMAEYDVSKNVIAKLEGSKRSDEYIIYTAHWDHLGIGKPDETGDSIYNGALDNASGTAALLEIARAFKQADEKPERTVVFLAVTAEEQGLWGSAYYAENPVFPKEKTVANINMDGINPYGKMKDIVLVGMGQSELEETLTDEADKVGRYTSPEPTPSAGYYFRSDHFNFAKIGIPA 467
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: UPI0019D408C1 (M28 family metallopeptidase n=1 Tax=Algoriphagus aestuariicola TaxID=1852016 RepID=UPI0019D408C1)

HSP 1 Score: 265 bits (676), Expect = 2.800e-83
Identity = 124/181 (68.51%), Postives = 154/181 (85.08%), Query Frame = 1 
Query:    4 SFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            S S+  + KY+ S N+VAK+TGKT+PDEYL+YS HWDH GIGK D+TGD+IYNGALDNASG+  +L +AKAF +   +P RTVVFL VTAEEQGL GS++YAQNPIYP EKTVANIN+DG+NPYGK +D+ ++G+GQS++ED L+ E +++G Y APEPNPVAGYYFRSDHF+FAKVGIPA
Sbjct:  282 SASIEVEAKYNVSKNVVAKVTGKTRPDEYLIYSAHWDHFGIGKPDETGDSIYNGALDNASGTAALLALAKAFKT-DGQPERTVVFLAVTAEEQGLWGSAFYAQNPIYPIEKTVANINMDGINPYGKMRDVSIIGVGQSEMEDLLNAELEKLGRYSAPEPNPVAGYYFRSDHFNFAKVGIPA 461
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BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Match: UPI00094B8527 (M28 family metallopeptidase n=1 Tax=Algoriphagus marinus TaxID=1925762 RepID=UPI00094B8527)

HSP 1 Score: 264 bits (675), Expect = 3.760e-83
Identity = 123/181 (67.96%), Postives = 151/181 (83.43%), Query Frame = 1 
Query:    4 SFSMSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYNGALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQNPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGSYVAPEPNPVAGYYFRSDHFSFAKVGIPA 546
            S S++ +  YD S N++AK+TG T+P+EY++YS HWDHLGIGK D+TGD+IYNGALDNA+G+  +L +AKAF S   KP RTV+FL VTAEEQGL GS YYA NPIYPKEKTVANIN+DG+NPYGK KD+ ++G+GQSD+ED L+ E ++VG Y AP+P P AGYYFRSDHFSFAKVGIPA
Sbjct:  281 SSSLAVEANYDVSKNVIAKVTGSTKPEEYIIYSAHWDHLGIGKPDETGDSIYNGALDNATGTAALLALAKAF-STDEKPERTVIFLAVTAEEQGLWGSLYYALNPIYPKEKTVANINMDGINPYGKMKDVSIIGVGQSDMEDLLNVELEKVGRYSAPDPTPTAGYYFRSDHFSFAKVGIPA 460
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig52136.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7L9BHV4_9BACT8.370e-9676.37M20/M25/M40 family metallo-hydrolase n=1 Tax=Flamm... [more]
UPI001FA7870B2.210e-8569.23M28 family metallopeptidase n=1 Tax=Pontibacter sp... [more]
UPI001FA752042.510e-8570.88M28 family metallopeptidase n=1 Tax=Pontibacter sp... [more]
A0A1N6TXX0_9BACT4.670e-8468.51Zn-dependent amino-or carboxypeptidase, M28 family... [more]
UPI00117D76245.680e-8468.51M28 family metallopeptidase n=1 Tax=Litoribacter p... [more]
A0A074KXV5_9BACT5.820e-8471.10Peptidase M28 n=1 Tax=Anditalea andensis TaxID=104... [more]
A0A142ELU3_9BACT6.890e-8467.58Peptidase M28 n=1 Tax=Algoriphagus sanaruensis Tax... [more]
UPI001BD209988.010e-8467.96M28 family metallopeptidase n=2 Tax=Litoribacter T... [more]
UPI0019D408C12.800e-8368.51M28 family metallopeptidase n=1 Tax=Algoriphagus a... [more]
UPI00094B85273.760e-8367.96M28 family metallopeptidase n=1 Tax=Algoriphagus m... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig52136contigP-wetherbeei_contig52136:1..546 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog1048983.EL17_16995
PFAMsPA,Peptidase_M28
Model size546
Max annot lvl976|Bacteroidetes
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.15e-84
EggNOG OGsCOG2234@1|root,COG2234@2|Bacteria,4NFDJ@976|Bacteroidetes,47KZX@768503|Cytophagia
DescriptionPA domain
Cds size534
COG categoryS
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186781.8839827-UTR-P-wetherbeei_contig52136:0..121683186781.8839827-UTR-P-wetherbeei_contig52136:0..12Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig52136 1..12 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186781.9046416-CDS-P-wetherbeei_contig52136:12..5461683186781.9046416-CDS-P-wetherbeei_contig52136:12..546Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig52136 13..546 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig52136.1.1prot_P-wetherbeei_contig52136.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig52136 13..546 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig52136.1.1

>prot_P-wetherbeei_contig52136.1.1 ID=prot_P-wetherbeei_contig52136.1.1|Name=mRNA_P-wetherbeei_contig52136.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=178bp
MSAKIKYDKSYNLVAKITGKTQPDEYLLYSTHWDHLGIGKADDTGDTIYN
GALDNASGSVGVLEIAKAFLSLKNKPARTVVFLWVTAEEQGLLGSSYYAQ
NPIYPKEKTVANINIDGVNPYGKNKDIVLVGMGQSDLEDYLSEEAKRVGS
YVAPEPNPVAGYYFRSDHFSFAKVGIPA
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mRNA from alignment at P-wetherbeei_contig52136:1..546+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig52136.1.1 ID=mRNA_P-wetherbeei_contig52136.1.1|Name=mRNA_P-wetherbeei_contig52136.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=546bp|location=Sequence derived from alignment at P-wetherbeei_contig52136:1..546+ (Phaeothamnion wetherbeei SAG_119_79)
GTTTCATTCTCGATGAGCGCTAAAATAAAATATGACAAATCGTATAATCT
TGTAGCCAAGATTACGGGCAAAACGCAACCTGATGAATATCTATTATACT
CAACGCATTGGGATCACCTGGGTATCGGTAAAGCCGATGATACAGGCGAC
ACTATATATAATGGAGCGTTAGATAATGCCAGCGGTTCAGTGGGCGTACT
TGAAATTGCAAAAGCTTTTCTTTCCTTAAAGAATAAGCCTGCACGAACTG
TAGTGTTCTTATGGGTAACAGCCGAAGAGCAAGGTTTGCTGGGTTCATCG
TATTACGCTCAGAATCCTATTTATCCAAAAGAGAAAACGGTTGCCAACAT
TAACATTGATGGCGTTAATCCTTACGGAAAAAATAAAGATATCGTGCTTG
TTGGTATGGGACAATCTGATCTGGAAGATTATTTAAGCGAAGAAGCAAAA
CGTGTAGGTAGTTACGTTGCACCTGAACCTAATCCGGTTGCCGGGTACTA
TTTCCGTTCTGATCACTTCAGCTTTGCCAAAGTCGGTATCCCAGCA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig52136:1..546+

>mRNA_P-wetherbeei_contig52136.1.1 ID=mRNA_P-wetherbeei_contig52136.1.1|Name=mRNA_P-wetherbeei_contig52136.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=534bp|location=Sequence derived from alignment at P-wetherbeei_contig52136:1..546+ (Phaeothamnion wetherbeei SAG_119_79)
ATGAGCGCTAAAATAAAATATGACAAATCGTATAATCTTGTAGCCAAGAT
TACGGGCAAAACGCAACCTGATGAATATCTATTATACTCAACGCATTGGG
ATCACCTGGGTATCGGTAAAGCCGATGATACAGGCGACACTATATATAAT
GGAGCGTTAGATAATGCCAGCGGTTCAGTGGGCGTACTTGAAATTGCAAA
AGCTTTTCTTTCCTTAAAGAATAAGCCTGCACGAACTGTAGTGTTCTTAT
GGGTAACAGCCGAAGAGCAAGGTTTGCTGGGTTCATCGTATTACGCTCAG
AATCCTATTTATCCAAAAGAGAAAACGGTTGCCAACATTAACATTGATGG
CGTTAATCCTTACGGAAAAAATAAAGATATCGTGCTTGTTGGTATGGGAC
AATCTGATCTGGAAGATTATTTAAGCGAAGAAGCAAAACGTGTAGGTAGT
TACGTTGCACCTGAACCTAATCCGGTTGCCGGGTACTATTTCCGTTCTGA
TCACTTCAGCTTTGCCAAAGTCGGTATCCCAGCA
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