mRNA_P-wetherbeei_contig51188.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig51188.1.1
Unique NamemRNA_P-wetherbeei_contig51188.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A3C1L361_9ACTN (Uncharacterized protein (Fragment) n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A3C1L361_9ACTN)

HSP 1 Score: 292 bits (748), Expect = 2.430e-95
Identity = 153/182 (84.07%), Postives = 161/182 (88.46%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLLA 546
            YG VRGT    LR VFDGLV+RVLAGA MACRSLDD AAAAMVERLAGVQAALALTDH ARR EWPAVLT+IAER DVHGLVQGRA RLLHDGG W R QVGNR+SRALS GT PAVGA+FVEGF+AGSGTVLVHD +LLDVIDAWVSSL PD+F+AT PLLRRTFG FEPAERRQLGMLLA
Sbjct:  227 YGDVRGTDAAALRRVFDGLVIRVLAGALMACRSLDDDAAAAMVERLAGVQAALALTDHQARRGEWPAVLTLIAERSDVHGLVQGRAARLLHDGGAWGRAQVGNRVSRALSTGTPPAVGASFVEGFVAGSGTVLVHDRDLLDVIDAWVSSLAPDSFVATVPLLRRTFGGFEPAERRQLGMLLA 408
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A6J7AT58_9ZZZZ (Unannotated protein n=1 Tax=freshwater metagenome TaxID=449393 RepID=A0A6J7AT58_9ZZZZ)

HSP 1 Score: 288 bits (736), Expect = 6.300e-95
Identity = 150/182 (82.42%), Postives = 160/182 (87.91%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLLA 546
            YG VRGT    LR VFDGLVVRVLAGA MACRSLDD AAAAMV+RLAG QAALALTDHPARR EWPA+L +++ER DVHGLVQGRATRLLHDGG W+R QVGNR+SRALSVGT  AVGAAFVEGFLAGSGTVLVHD ELLDVID WVS L PDAF++T PLLRRTFGAFE AERRQLG+LLA
Sbjct:  122 YGDVRGTDASALRHVFDGLVVRVLAGALMACRSLDDDAAAAMVDRLAGAQAALALTDHPARRGEWPALLAIVSERGDVHGLVQGRATRLLHDGGAWKRSQVGNRVSRALSVGTPSAVGAAFVEGFLAGSGTVLVHDRELLDVIDTWVSGLAPDAFLSTVPLLRRTFGAFEQAERRQLGLLLA 303
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A7X9Q2T1_9ACTN (Uncharacterized protein n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A7X9Q2T1_9ACTN)

HSP 1 Score: 291 bits (745), Expect = 1.110e-91
Identity = 152/182 (83.52%), Postives = 161/182 (88.46%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLLA 546
            YG VRGT    LR VFDGLV+RVLAGA MACRSLDD AAAAMVERLAGVQAALALTDH ARR EWPAVLT+IAER DVHGLVQGRA RLLHDGG W R QVGNR+SRALS GT PAVGA+FVEGF+AGSGTVLVHD +LLDVIDAWVSSL PD+F+AT PLLRRTFG FEPAERRQLG+LLA
Sbjct:  517 YGDVRGTDAAALRRVFDGLVIRVLAGALMACRSLDDDAAAAMVERLAGVQAALALTDHQARRGEWPAVLTLIAERSDVHGLVQGRAARLLHDGGAWGRAQVGNRVSRALSTGTPPAVGASFVEGFVAGSGTVLVHDRDLLDVIDAWVSSLAPDSFVATVPLLRRTFGGFEPAERRQLGLLLA 698
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A6I3EWK3_9ACTN (Uncharacterized protein n=2 Tax=root TaxID=1 RepID=A0A6I3EWK3_9ACTN)

HSP 1 Score: 288 bits (736), Expect = 2.590e-90
Identity = 150/182 (82.42%), Postives = 160/182 (87.91%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLLA 546
            YG VRGT    LR VFDGLVVRVLAGA MACRSLDD AAAAMV+RLAG QAALALTDHPARR EWPA+L +++ER DVHGLVQGRATRLLHDGG W+R QVGNR+SRALSVGT  AVGAAFVEGFLAGSGTVLVHD ELLDVID WVS L PDAF++T PLLRRTFGAFE AERRQLG+LLA
Sbjct:  521 YGDVRGTDASALRHVFDGLVVRVLAGALMACRSLDDDAAAAMVDRLAGAQAALALTDHPARRGEWPALLAIVSERGDVHGLVQGRATRLLHDGGAWKRSQVGNRVSRALSVGTPSAVGAAFVEGFLAGSGTVLVHDRELLDVIDTWVSGLAPDAFLSTVPLLRRTFGAFEQAERRQLGLLLA 702
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A7W0KXI6_9ACTN (Uncharacterized protein n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A7W0KXI6_9ACTN)

HSP 1 Score: 209 bits (531), Expect = 4.590e-65
Identity = 112/182 (61.54%), Postives = 134/182 (73.63%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLLA 546
            YG  R T    LR+VFD + VRV+AG   A   LDD +A AM+ER++ VQAAL++ DHPARR   PAVL +IA++   HGLV+GRATRLLHD G W+  +V  RL+RAL+ GT  A GAAFVEGFLAGSGTVL+HDA L  V+D W+ SL PDAF     LLRRTFGAFEP ERRQLG+L+A
Sbjct:   27 YGDARSTDAAALRAVFDEMAVRVVAGLDRAVVGLDDESARAMIERMSAVQAALSVVDHPARRRALPAVLAIIADQRRGHGLVRGRATRLLHDTGTWEPAEVERRLARALTPGTPAATGAAFVEGFLAGSGTVLLHDAALRTVVDGWIGSLTPDAFTDVVALLRRTFGAFEPVERRQLGVLVA 208
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A2E3TVY6_9ACTN (Uncharacterized protein n=1 Tax=Ilumatobacter sp. TaxID=1967498 RepID=A0A2E3TVY6_9ACTN)

HSP 1 Score: 221 bits (563), Expect = 6.730e-65
Identity = 122/181 (67.40%), Postives = 141/181 (77.90%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLL 543
            YG VRGT    +R+VFDG+VVR+LAGAA+AC  LDD AA  MVERLAGVQ+ALA+  H AR  ++PA+L  +A   +VHGLVQGRATRLLHDGG WQ   V NRLS+ALS GT   VGAAF+EGFLAGSGTVLVHDA+LL V+D W+SSL P AF A   LLRRTFG FE A+RRQ+ MLL
Sbjct:  519 YGDVRGTDASLMRTVFDGIVVRILAGAAVACGQLDDDAAVEMVERLAGVQSALAVLGHHAREHDFPALLDQLASARNVHGLVQGRATRLLHDGGSWQAIDVENRLSQALSPGTPSVVGAAFIEGFLAGSGTVLVHDADLLGVVDRWLSSLTPVAFDAALVLLRRTFGGFESAQRRQIMMLL 699
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: UPI00156374D1 (DUF5682 family protein n=1 Tax=Ilumatobacter nonamiensis TaxID=467093 RepID=UPI00156374D1)

HSP 1 Score: 216 bits (549), Expect = 5.990e-63
Identity = 124/182 (68.13%), Postives = 135/182 (74.18%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDV-HGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLL 543
            YG VRG+    LR VFDGLVVRVLAG   AC SLDD AAA MVERL  +Q ALA+ DH ARR  +PAVL  +A R  + HGL+ GRATRLLHD G W    V  RLS+ALS GT P VGAAFVEGFLAGSGTVLVHDA LL V+D W+ SL PDAF AT  LLRRTFGAFEPAERRQ+  LL
Sbjct:  511 YGDVRGSDAGSLRDVFDGLVVRVLAGLVPACSSLDDDAAARMVERLTAMQTALAMLDHDARRQAFPAVLEQLAPRHAMRHGLICGRATRLLHDAGRWDEHVVERRLSQALSGGTPPDVGAAFVEGFLAGSGTVLVHDAALLAVVDRWLCSLTPDAFEATVALLRRTFGAFEPAERRQIMSLL 692
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A4R7I4G2_9ACTN (Uncharacterized protein n=1 Tax=Ilumatobacter fluminis TaxID=467091 RepID=A0A4R7I4G2_9ACTN)

HSP 1 Score: 215 bits (548), Expect = 8.830e-63
Identity = 124/182 (68.13%), Postives = 132/182 (72.53%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLLA 546
            YG VR T    L SVFDGLVVRVLAG   AC SLDD AAA MVERL+  Q ALAL DH ARR  +P VL  +AE    HGLV GRATR+LHD G W  G V  RL RALS GT PA GAAFVEGFLAGSG VLVHD +LL V+D W+SSL P AF  T PLLRRTFGAFE AERRQLG L+A
Sbjct:  513 YGDVRTTDRDALASVFDGLVVRVLAGLVPACASLDDDAAALMVERLSEAQQALALVDHAARRRAYPEVLEQLAEIAG-HGLVHGRATRILHDAGRWDSGTVERRLGRALSGGTPPAAGAAFVEGFLAGSGAVLVHDTDLLAVVDRWLSSLAPQAFDETVPLLRRTFGAFESAERRQLGRLVA 693
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A2E0RM23_9ACTN (Uncharacterized protein n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2E0RM23_9ACTN)

HSP 1 Score: 215 bits (547), Expect = 1.450e-62
Identity = 123/182 (67.58%), Postives = 133/182 (73.08%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLLA 546
            YG VR T    L SVFDGLVVRVLAG A AC SLDD AA  MVERL+    ALAL +HPAR   +P VL  +A     HGLVQGRATR+LHD G W    V +RL RALS GT PAVGAAFVEGFLAGSGTVLVHD +LL V+D W+SSL P AF  T PLLRRTFGAFE AERRQLG L+A
Sbjct:  521 YGDVRATDREALASVFDGLVVRVLAGLAPACASLDDEAATLMVERLSEAHQALALVEHPARHRAYPEVLEQLAATAG-HGLVQGRATRILHDAGRWGSSTVEHRLGRALSGGTPPAVGAAFVEGFLAGSGTVLVHDTDLLAVVDRWLSSLRPQAFEETVPLLRRTFGAFESAERRQLGRLVA 701
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BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Match: A0A6C7E6B0_ILUCY (Uncharacterized protein n=1 Tax=Ilumatobacter coccineus (strain NBRC 103263 / KCTC 29153 / YM16-304) TaxID=1313172 RepID=A0A6C7E6B0_ILUCY)

HSP 1 Score: 214 bits (545), Expect = 3.950e-62
Identity = 124/187 (66.31%), Postives = 132/187 (70.59%), Query Frame = 1 
Query:    1 YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQAALALTDHPARRAEWPAVLTVIAERPDVH------GLVQGRATRLLHDGGVWQRGQVGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSLGPDAFIATAPLLRRTFGAFEPAERRQLGMLL 543
            YG VR T    LR VFDGLVVRVLA    AC SLDD AAA MVERL  VQ AL++ DHPARR  +P VL  +AER          GL+ GRATRLLHDG VW  GQV  RL RALS GT P VGAAFVEGFLAGSGTVLVHD ELL V+D W+SSL P+AF A   LLRRTFG FE AERRQL  LL
Sbjct:  532 YGDVRATDASSLRHVFDGLVVRVLAALVPACSSLDDDAAAQMVERLTTVQTALSMLDHPARRQAFPRVLAQLAERSGERSESSGRGLIAGRATRLLHDGDVWDAGQVERRLGRALSPGTPPLVGAAFVEGFLAGSGTVLVHDTELLGVVDRWLSSLRPEAFEAVMALLRRTFGGFEAAERRQLMALL 718
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig51188.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3C1L361_9ACTN2.430e-9584.07Uncharacterized protein (Fragment) n=1 Tax=Acidimi... [more]
A0A6J7AT58_9ZZZZ6.300e-9582.42Unannotated protein n=1 Tax=freshwater metagenome ... [more]
A0A7X9Q2T1_9ACTN1.110e-9183.52Uncharacterized protein n=1 Tax=Actinomycetia bact... [more]
A0A6I3EWK3_9ACTN2.590e-9082.42Uncharacterized protein n=2 Tax=root TaxID=1 RepID... [more]
A0A7W0KXI6_9ACTN4.590e-6561.54Uncharacterized protein n=1 Tax=Acidimicrobiia bac... [more]
A0A2E3TVY6_9ACTN6.730e-6567.40Uncharacterized protein n=1 Tax=Ilumatobacter sp. ... [more]
UPI00156374D15.990e-6368.13DUF5682 family protein n=1 Tax=Ilumatobacter nonam... [more]
A0A4R7I4G2_9ACTN8.830e-6368.13Uncharacterized protein n=1 Tax=Ilumatobacter flum... [more]
A0A2E0RM23_9ACTN1.450e-6267.58Uncharacterized protein n=1 Tax=Acidimicrobiaceae ... [more]
A0A6C7E6B0_ILUCY3.950e-6266.31Uncharacterized protein n=1 Tax=Ilumatobacter cocc... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig51188contigP-wetherbeei_contig51188:6..551 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1313172.YM304_29500
Model size546
Max annot lvl201174|Actinobacteria
Hectar predicted targeting categoryother localisation
Exons1
Evalue6.47e-63
EggNOG OGsCOG1916@1|root,COG1916@2|Bacteria,2H79D@201174|Actinobacteria
DescriptionNon-essential cell division protein that could be required for efficient cell constriction
Cds size546
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186769.3926663-CDS-P-wetherbeei_contig51188:5..5511683186769.3926663-CDS-P-wetherbeei_contig51188:5..551Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig51188 6..551 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig51188.1.1prot_P-wetherbeei_contig51188.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig51188 6..551 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig51188.1.1

>prot_P-wetherbeei_contig51188.1.1 ID=prot_P-wetherbeei_contig51188.1.1|Name=mRNA_P-wetherbeei_contig51188.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=182bp
YGAVRGTGGWGLRSVFDGLVVRVLAGAAMACRSLDDAAAAAMVERLAGVQ
AALALTDHPARRAEWPAVLTVIAERPDVHGLVQGRATRLLHDGGVWQRGQ
VGNRLSRALSVGTTPAVGAAFVEGFLAGSGTVLVHDAELLDVIDAWVSSL
GPDAFIATAPLLRRTFGAFEPAERRQLGMLLA
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mRNA from alignment at P-wetherbeei_contig51188:6..551-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig51188.1.1 ID=mRNA_P-wetherbeei_contig51188.1.1|Name=mRNA_P-wetherbeei_contig51188.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=546bp|location=Sequence derived from alignment at P-wetherbeei_contig51188:6..551- (Phaeothamnion wetherbeei SAG_119_79)
TACGGCGCGGTGCGCGGGACCGGTGGCTGGGGGTTGCGATCGGTGTTCGA
CGGATTGGTCGTGCGCGTGCTGGCAGGTGCAGCGATGGCGTGCCGCTCCC
TCGATGACGCCGCCGCGGCTGCGATGGTCGAACGGCTCGCCGGAGTGCAG
GCAGCGTTGGCGCTCACCGACCACCCGGCGCGCCGAGCCGAGTGGCCCGC
CGTGCTGACGGTGATCGCCGAGCGACCGGATGTGCACGGATTGGTGCAGG
GGCGCGCGACGCGGCTGCTGCACGACGGCGGCGTGTGGCAGCGCGGGCAA
GTGGGCAACCGCCTGTCGCGTGCGCTCAGCGTCGGCACCACGCCTGCCGT
CGGTGCCGCGTTCGTCGAGGGCTTCCTGGCCGGCAGCGGCACCGTGCTCG
TCCACGACGCGGAGCTCCTCGACGTGATCGACGCCTGGGTGTCGTCGCTC
GGCCCGGATGCGTTCATCGCGACGGCGCCCTTGCTGCGCCGCACGTTCGG
CGCGTTCGAGCCGGCCGAGCGTCGCCAGTTGGGCATGCTGCTCGCC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig51188:6..551-

>mRNA_P-wetherbeei_contig51188.1.1 ID=mRNA_P-wetherbeei_contig51188.1.1|Name=mRNA_P-wetherbeei_contig51188.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=546bp|location=Sequence derived from alignment at P-wetherbeei_contig51188:6..551- (Phaeothamnion wetherbeei SAG_119_79)
TACGGCGCGGTGCGCGGGACCGGTGGCTGGGGGTTGCGATCGGTGTTCGA
CGGATTGGTCGTGCGCGTGCTGGCAGGTGCAGCGATGGCGTGCCGCTCCC
TCGATGACGCCGCCGCGGCTGCGATGGTCGAACGGCTCGCCGGAGTGCAG
GCAGCGTTGGCGCTCACCGACCACCCGGCGCGCCGAGCCGAGTGGCCCGC
CGTGCTGACGGTGATCGCCGAGCGACCGGATGTGCACGGATTGGTGCAGG
GGCGCGCGACGCGGCTGCTGCACGACGGCGGCGTGTGGCAGCGCGGGCAA
GTGGGCAACCGCCTGTCGCGTGCGCTCAGCGTCGGCACCACGCCTGCCGT
CGGTGCCGCGTTCGTCGAGGGCTTCCTGGCCGGCAGCGGCACCGTGCTCG
TCCACGACGCGGAGCTCCTCGACGTGATCGACGCCTGGGTGTCGTCGCTC
GGCCCGGATGCGTTCATCGCGACGGCGCCCTTGCTGCGCCGCACGTTCGG
CGCGTTCGAGCCGGCCGAGCGTCGCCAGTTGGGCATGCTGCTCGCC
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