mRNA_P-wetherbeei_contig17994.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig17994.1.1
Unique NamemRNA_P-wetherbeei_contig17994.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: A0A1F4CXY1_9PROT (Holliday junction ATP-dependent DNA helicase RuvA n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_12_FULL_68_19 TaxID=1797504 RepID=A0A1F4CXY1_9PROT)

HSP 1 Score: 234 bits (597), Expect = 2.870e-76
Identity = 126/161 (78.26%), Postives = 137/161 (85.09%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPASDVLNALLALGYS 486
            IGRLTG LAAK+PPQV+VD GGV YE+DVPMSTFYNLPA+GE V L THLVVREDAH+L+GF T EERSAFR+LIRISGVGARTAL+VLSGLSV DLA AV  QDG+RL KVPGIGKKTAERLLLELKGKLA+ LPG      A   ASDV+NALL LGYS
Sbjct:    2 IGRLTGRLAAKHPPQVLVDVGGVAYEVDVPMSTFYNLPASGEAVSLFTHLVVREDAHVLYGFATLEERSAFRQLIRISGVGARTALAVLSGLSVADLAQAVMLQDGSRLVKVPGIGKKTAERLLLELKGKLAEALPGP-----AQERASDVVNALLGLGYS 157
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: UPI00193BDDC1 (Holliday junction branch migration protein RuvA n=1 Tax=Azospira restricta TaxID=404405 RepID=UPI00193BDDC1)

HSP 1 Score: 234 bits (597), Expect = 3.390e-76
Identity = 120/161 (74.53%), Postives = 135/161 (83.85%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPASDVLNALLALGYS 486
            IGRLTG LAAK PPQ++VD  GVGYE+DVPMSTFYNLPA G+KVVLLTH  VRED H L+GFG+E ER AFR+L++ISGVGARTAL+VLSGLSV DLA AVA Q+  RLTKVPGIGKKTAERLLLELKGKLA+ +P A  A AA     D+LNALLALGY+
Sbjct:    2 IGRLTGTLAAKTPPQILVDVNGVGYEVDVPMSTFYNLPAAGDKVVLLTHHAVREDGHFLYGFGSEAERFAFRQLLKISGVGARTALAVLSGLSVADLAQAVAAQEAGRLTKVPGIGKKTAERLLLELKGKLAEAVPAAAAAAAAGDSRHDILNALLALGYN 162
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: UPI0019A286DF (Holliday junction branch migration protein RuvA n=1 Tax=Candidatus Parcubacteria bacterium TaxID=2762014 RepID=UPI0019A286DF)

HSP 1 Score: 231 bits (588), Expect = 6.950e-75
Identity = 124/161 (77.02%), Postives = 131/161 (81.37%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPASDVLNALLALGYS 486
            IGRL+G L +KNPPQV++D  GVGYEIDVPMSTFYNLPATGE   L THLVVREDAH+L+GF T EERS FRKLIRISGVGARTALSVLSGLSV DLA AV  QD  RLTKVPGIGKKTAERLLLELKGKL+D LP     G      SDVLNALL LGYS
Sbjct:    2 IGRLSGTLVSKNPPQVLLDVAGVGYEIDVPMSTFYNLPATGEAATLHTHLVVREDAHVLYGFATLEERSVFRKLIRISGVGARTALSVLSGLSVADLAQAVTLQDTGRLTKVPGIGKKTAERLLLELKGKLSDTLPAETAKGGG----SDVLNALLGLGYS 158
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: A0A8J3AXM3_9BURK (Holliday junction ATP-dependent DNA helicase RuvA n=3 Tax=Oxalicibacterium TaxID=223927 RepID=A0A8J3AXM3_9BURK)

HSP 1 Score: 230 bits (586), Expect = 1.600e-74
Identity = 118/161 (73.29%), Postives = 135/161 (83.85%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPASDVLNALLALGYS 486
            IGRL+G+L  KNPPQ++VD  GVGYE++VPMSTFYNLP   EKV LLTHLVVREDAHILFGFGT EER+ F++LI+ISG+GARTALS+LSG+SV DLA AV  Q+  RLTKVPGIGKKTAERLLLELKGKL   +   G A AA+  +SDVLNALLALGYS
Sbjct:    2 IGRLSGILLEKNPPQLLVDCNGVGYEVNVPMSTFYNLPGLNEKVALLTHLVVREDAHILFGFGTSEERNTFKELIKISGIGARTALSILSGMSVADLAQAVTLQEAGRLTKVPGIGKKTAERLLLELKGKLGADIGATGGAAAASDTSSDVLNALLALGYS 162
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: A0A401JFF7_9PROT (Holliday junction ATP-dependent DNA helicase RuvA n=2 Tax=Sulfuriferula TaxID=1778653 RepID=A0A401JFF7_9PROT)

HSP 1 Score: 229 bits (584), Expect = 3.120e-74
Identity = 118/161 (73.29%), Postives = 138/161 (85.71%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPASDVLNALLALGYS 486
            IGR+TG L  K+PPQ++VDA GVGYEIDVPMSTFYNLPA GE+V L THLVVREDA +L+GFG+E ER+ FR+LI+I+GVGA+ AL+VLSG++ N+LA AVA QD  RLTK+PGIGKKTAERLLLELKGKLAD LPGAG +G AA    DVLNALLALGY+
Sbjct:    2 IGRITGTLIEKHPPQIVVDAHGVGYEIDVPMSTFYNLPAIGERVALHTHLVVREDAQLLYGFGSEAERATFRQLIKITGVGAKMALAVLSGMNANELAQAVAMQDTGRLTKIPGIGKKTAERLLLELKGKLADALPGAGQSGVAAA-GGDVLNALLALGYN 161
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: A0A522VXB2_9PROT (Holliday junction ATP-dependent DNA helicase RuvA n=2 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A522VXB2_9PROT)

HSP 1 Score: 229 bits (584), Expect = 3.220e-74
Identity = 116/161 (72.05%), Postives = 139/161 (86.34%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPASDVLNALLALGYS 486
            IGRLTG L A++PPQV++D GGV YE+DVPMSTFYNLP TGE V L THL+VREDAH L+GF T +ER+AFR+LIRISG+GARTAL+VLSGLSV++LA AVA Q+ ARLTKVPGIGKKTAERLLLELKG+L + LPG+G A A  + ++D+L+ALL LGYS
Sbjct:    2 IGRLTGRLVARHPPQVLLDVGGVAYEVDVPMSTFYNLPGTGEAVTLHTHLLVREDAHTLYGFATLDERTAFRQLIRISGIGARTALAVLSGLSVSELAQAVALQESARLTKVPGIGKKTAERLLLELKGRLVEALPGSGAAAAPDSSSADILHALLGLGYS 162
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: A0A5C7K2M7_9RHOO (Holliday junction ATP-dependent DNA helicase RuvA n=1 Tax=Rhodocyclaceae bacterium TaxID=1898103 RepID=A0A5C7K2M7_9RHOO)

HSP 1 Score: 228 bits (582), Expect = 6.280e-74
Identity = 120/161 (74.53%), Postives = 135/161 (83.85%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPASDVLNALLALGYS 486
            IGRL+G LA KNPPQ++VD GGVGYE+DVPMSTFYNLPA+GEKV LLTH  VRED H L+GFG+E ER AFR+L++ISGVGARTALSVLSGLSV DLA AVA Q+  RLTKVPGIGKKTAERLLLELKGKLAD +P A  A   +    D+LNALLALGY+
Sbjct:    2 IGRLSGTLAEKNPPQILVDVGGVGYEVDVPMSTFYNLPASGEKVTLLTHHAVREDGHFLYGFGSEAERFAFRQLLKISGVGARTALSVLSGLSVADLAQAVAMQEAGRLTKVPGIGKKTAERLLLELKGKLADTVPNA-TALVQSDSRHDILNALLALGYN 161
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: A0A521Z5W1_9PROT (Holliday junction ATP-dependent DNA helicase RuvA n=4 Tax=Bacteria TaxID=2 RepID=A0A521Z5W1_9PROT)

HSP 1 Score: 227 bits (578), Expect = 2.240e-73
Identity = 123/165 (74.55%), Postives = 137/165 (83.03%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPA----SDVLNALLALGYS 486
            IGR++G LA K PPQV+VD GGV YE+DVPMSTFY+LPATGE + L THL+VREDAH+L+GF T EERSAFR+LIRISGVGARTAL+VLSGLSV DLA AV  QD ARLT+VPGIGKKTAERLLLELKGKLA+V         AATPA    SD+LNALLALGYS
Sbjct:    2 IGRISGKLAVKQPPQVLVDVGGVAYELDVPMSTFYSLPATGEPITLYTHLIVREDAHMLYGFATLEERSAFRQLIRISGVGARTALAVLSGLSVADLAQAVVMQDAARLTRVPGIGKKTAERLLLELKGKLAEV---------AATPAQEHASDILNALLALGYS 157
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: A0A3S9HGX9_9BURK (Holliday junction ATP-dependent DNA helicase RuvA n=15 Tax=Burkholderiales TaxID=80840 RepID=A0A3S9HGX9_9BURK)

HSP 1 Score: 227 bits (578), Expect = 2.640e-73
Identity = 115/161 (71.43%), Postives = 135/161 (83.85%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPASDVLNALLALGYS 486
            IGR+ G+L  KNPPQ++VD  GVGYEIDVPMSTFYNLPA GEKV+LLTHL +REDAH+LFGFG+ EER+ FR+LI+ISGVG+RTAL++LSG+SV DLA A+  Q+  RLT+VPGIGKKTAERLLLELKGKL   L  A  A AA   +SD+LNALLALGYS
Sbjct:    2 IGRIAGILIEKNPPQLLVDCQGVGYEIDVPMSTFYNLPALGEKVILLTHLAIREDAHVLFGFGSSEERATFRQLIKISGVGSRTALAILSGMSVADLAQAITLQEAGRLTRVPGIGKKTAERLLLELKGKLGADLGAASAAIAAGDHSSDILNALLALGYS 162
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BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Match: A0A6N6LG20_9PROT (Holliday junction ATP-dependent DNA helicase RuvA n=4 Tax=Proteobacteria TaxID=1224 RepID=A0A6N6LG20_9PROT)

HSP 1 Score: 227 bits (578), Expect = 3.210e-73
Identity = 115/167 (68.86%), Postives = 137/167 (82.04%), Query Frame = 1 
Query:    4 IGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTHLVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAHAVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLP------GAGPAGAAATPASDVLNALLALGYS 486
            IGRLTG L  KNPPQ++VDAGGVGYE+DVPMSTFY+LPATG+KV L THL +RED H L+GFG++EER+AFR+LI++SG+GARTAL+VLSGLSV DLA A+A Q+  RL K+PGIGKKTAERLLLELK KL   LP      G+  AGAA    SD+LNAL+ALGY+
Sbjct:    2 IGRLTGTLLEKNPPQILVDAGGVGYEVDVPMSTFYHLPATGQKVSLHTHLAIREDGHFLYGFGSDEERAAFRQLIKVSGIGARTALAVLSGLSVTDLAQAIALQEAGRLVKIPGIGKKTAERLLLELKDKLGKALPTVAASLGSSGAGAAPDARSDILNALMALGYN 168
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig17994.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1F4CXY1_9PROT2.870e-7678.26Holliday junction ATP-dependent DNA helicase RuvA ... [more]
UPI00193BDDC13.390e-7674.53Holliday junction branch migration protein RuvA n=... [more]
UPI0019A286DF6.950e-7577.02Holliday junction branch migration protein RuvA n=... [more]
A0A8J3AXM3_9BURK1.600e-7473.29Holliday junction ATP-dependent DNA helicase RuvA ... [more]
A0A401JFF7_9PROT3.120e-7473.29Holliday junction ATP-dependent DNA helicase RuvA ... [more]
A0A522VXB2_9PROT3.220e-7472.05Holliday junction ATP-dependent DNA helicase RuvA ... [more]
A0A5C7K2M7_9RHOO6.280e-7474.53Holliday junction ATP-dependent DNA helicase RuvA ... [more]
A0A521Z5W1_9PROT2.240e-7374.55Holliday junction ATP-dependent DNA helicase RuvA ... [more]
A0A3S9HGX9_9BURK2.640e-7371.43Holliday junction ATP-dependent DNA helicase RuvA ... [more]
A0A6N6LG20_9PROT3.210e-7368.86Holliday junction ATP-dependent DNA helicase RuvA ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig17994contigP-wetherbeei_contig17994:2..487 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1121127.JAFA01000001_gene927
Preferred nameruvA
PFAMsHHH_5,RuvA_C,RuvA_N
Model size486
Max annot lvl28216|Betaproteobacteria
KEGG koko:K03550
KEGG Pathwayko03440,map03440
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.06e-74
EggNOG OGsCOG0632@1|root,COG0632@2|Bacteria,1MWJR@1224|Proteobacteria,2VJ98@28216|Betaproteobacteria,1K0EV@119060|Burkholderiaceae
EC3.6.4.12
DescriptionThe RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB
Cds size486
COG categoryL
BRITEko00000,ko00001,ko01000,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186132.9423566-CDS-P-wetherbeei_contig17994:1..4871683186132.9423566-CDS-P-wetherbeei_contig17994:1..487Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig17994 2..487 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig17994.1.1prot_P-wetherbeei_contig17994.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig17994 2..487 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig17994.1.1

>prot_P-wetherbeei_contig17994.1.1 ID=prot_P-wetherbeei_contig17994.1.1|Name=mRNA_P-wetherbeei_contig17994.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=162bp
VIGRLTGVLAAKNPPQVMVDAGGVGYEIDVPMSTFYNLPATGEKVVLLTH
LVVREDAHILFGFGTEEERSAFRKLIRISGVGARTALSVLSGLSVNDLAH
AVATQDGARLTKVPGIGKKTAERLLLELKGKLADVLPGAGPAGAAATPAS
DVLNALLALGYS
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mRNA from alignment at P-wetherbeei_contig17994:2..487-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig17994.1.1 ID=mRNA_P-wetherbeei_contig17994.1.1|Name=mRNA_P-wetherbeei_contig17994.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=486bp|location=Sequence derived from alignment at P-wetherbeei_contig17994:2..487- (Phaeothamnion wetherbeei SAG_119_79)
GTGATCGGCCGGCTAACAGGCGTACTGGCCGCCAAGAATCCCCCTCAGGT
GATGGTCGATGCGGGCGGCGTCGGGTACGAGATCGACGTGCCGATGAGCA
CTTTCTACAACCTGCCGGCCACCGGCGAGAAGGTCGTGCTGCTCACGCAC
CTCGTCGTGCGCGAGGACGCGCACATCCTGTTCGGCTTCGGCACCGAGGA
AGAGCGCTCGGCTTTCCGCAAGCTGATCCGCATCTCCGGCGTGGGTGCGC
GCACTGCGCTGTCCGTGCTCTCCGGCCTTTCGGTCAACGACCTTGCGCAT
GCGGTGGCCACGCAGGACGGCGCACGGCTCACCAAGGTGCCGGGCATAGG
CAAGAAGACCGCCGAGCGGCTGCTGCTCGAACTCAAGGGCAAGCTCGCCG
ACGTGCTGCCGGGCGCGGGTCCGGCCGGTGCCGCGGCGACGCCGGCCTCC
GATGTGCTGAACGCGCTGCTGGCACTGGGCTACAGC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig17994:2..487-

>mRNA_P-wetherbeei_contig17994.1.1 ID=mRNA_P-wetherbeei_contig17994.1.1|Name=mRNA_P-wetherbeei_contig17994.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=486bp|location=Sequence derived from alignment at P-wetherbeei_contig17994:2..487- (Phaeothamnion wetherbeei SAG_119_79)
GTGATCGGCCGGCTAACAGGCGTACTGGCCGCCAAGAATCCCCCTCAGGT
GATGGTCGATGCGGGCGGCGTCGGGTACGAGATCGACGTGCCGATGAGCA
CTTTCTACAACCTGCCGGCCACCGGCGAGAAGGTCGTGCTGCTCACGCAC
CTCGTCGTGCGCGAGGACGCGCACATCCTGTTCGGCTTCGGCACCGAGGA
AGAGCGCTCGGCTTTCCGCAAGCTGATCCGCATCTCCGGCGTGGGTGCGC
GCACTGCGCTGTCCGTGCTCTCCGGCCTTTCGGTCAACGACCTTGCGCAT
GCGGTGGCCACGCAGGACGGCGCACGGCTCACCAAGGTGCCGGGCATAGG
CAAGAAGACCGCCGAGCGGCTGCTGCTCGAACTCAAGGGCAAGCTCGCCG
ACGTGCTGCCGGGCGCGGGTCCGGCCGGTGCCGCGGCGACGCCGGCCTCC
GATGTGCTGAACGCGCTGCTGGCACTGGGCTACAGC
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