mRNA_P-wetherbeei_contig17542.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig17542.1.1
Unique NamemRNA_P-wetherbeei_contig17542.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A127ERL3_9HYPH (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Rhodoplanes sp. Z2-YC6860 TaxID=674703 RepID=A0A127ERL3_9HYPH)

HSP 1 Score: 235 bits (599), Expect = 3.220e-77
Identity = 113/151 (74.83%), Postives = 131/151 (86.75%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQT-GHKLE 456
            +ALYEPDIPQNTGTILRLAAC G++AHI+EPTGFPSSDRAFRRAGMDYLDQV+L+RH SW+AF+ WR++EGLR++LFTT A T YLD  FQ  D+L+FGRESAG PEAVHQAAD RL +PMR  LRS+NVA+ AAMA GEA+RQT G K E
Sbjct:    3 IALYEPDIPQNTGTILRLAACLGVEAHIVEPTGFPSSDRAFRRAGMDYLDQVSLRRHQSWAAFNAWRQSEGLRLLLFTTAAGTSYLDCAFQAGDILMFGRESAGAPEAVHQAADLRLKVPMRPNLRSINVAMTAAMALGEALRQTDGFKHE 153
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A537LDS2_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A537LDS2_9PROT)

HSP 1 Score: 226 bits (576), Expect = 9.620e-74
Identity = 111/146 (76.03%), Postives = 124/146 (84.93%), Query Frame = 1 
Query:    1 VSLALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQ 438
            + LALYEPDI QNTGTILRL AC G+ AHIIEP GFP +DRAFRRAGMDYLDQVTL+RH SW+ FD WRRA  LR+VLFTT A T YLDH++ G+DVLLFGRES+GVP+AVH AADARL IPMR GLRSLNVA+  AMA GEA+RQ
Sbjct:    1 MQLALYEPDIAQNTGTILRLCACLGVAAHIIEPAGFPVTDRAFRRAGMDYLDQVTLRRHVSWTGFDAWRRAGKLRLVLFTTRATTSYLDHRY-GDDVLLFGRESSGVPDAVHAAADARLTIPMRPGLRSLNVAMACAMAVGEALRQ 145
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A7C4NBY8_9CHLR (Putative tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A7C4NBY8_9CHLR)

HSP 1 Score: 226 bits (576), Expect = 1.430e-73
Identity = 107/145 (73.79%), Postives = 124/145 (85.52%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQT 441
            LALY+PDIPQNTGT+LRL AC GI+AHIIEP GF +SDRAFRRAG+DYLD V + RHTSW  F+ WRRA G ++VLFTT AATPYL+H+F  +DVLLFGRES+GVP+ VH+AAD RL IPMR G+RSLNVA+ AAMA GEAMRQT
Sbjct:    3 LALYQPDIPQNTGTLLRLCACLGIEAHIIEPAGFDTSDRAFRRAGLDYLDHVRIVRHTSWGTFEAWRRATGHKLVLFTTKAATPYLEHRFSTDDVLLFGRESSGVPDEVHRAADTRLRIPMRPGMRSLNVAISAAMAVGEAMRQT 147
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A537S0Q7_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=2 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A537S0Q7_9PROT)

HSP 1 Score: 222 bits (566), Expect = 3.670e-72
Identity = 105/151 (69.54%), Postives = 122/151 (80.79%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQTGHKLEG 459
            +ALYEPDIPQNTGTILRLAAC G++ HII+P GFP+SDRAFRRAGMDYLDQV + RH  W AF+TWR     R+++FTT A   YL+H F+  DVLLFGRE++GVPE VH AADARL+IPMR GLRSLNVA+ AAMA GEAMRQT  +  G
Sbjct:    3 IALYEPDIPQNTGTILRLAACLGVETHIIDPAGFPTSDRAFRRAGMDYLDQVEIARHADWDAFETWRAGAAARLIVFTTHARRSYLEHAFRRGDVLLFGRETSGVPEKVHAAADARLLIPMRQGLRSLNVAMAAAMAVGEAMRQTQAQASG 153
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A1M3C8H1_9BRAD (tRNA (cytidine(34)-2'-O)-methyltransferase n=2 Tax=unclassified Nitrobacter TaxID=2620411 RepID=A0A1M3C8H1_9BRAD)

HSP 1 Score: 221 bits (564), Expect = 7.640e-72
Identity = 107/150 (71.33%), Postives = 122/150 (81.33%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQTGHKLE 456
            +AL++PDIPQNTGTILRL AC  +QAHIIEP GFP SDR FRRAGMDYLDQVT+ RH SW  F  WR  +G R+VLFTT  A PYLDH +   D+LLFGRESAGVP+AV  AADARLVIP+R+GLRSLNVAV AAMA GEA+RQTG  ++
Sbjct:    3 IALFQPDIPQNTGTILRLCACLDLQAHIIEPAGFPVSDRHFRRAGMDYLDQVTIVRHDSWPKFQEWRAGDGGRLVLFTTRGALPYLDHCYAATDILLFGRESAGVPDAVAAAADARLVIPVRSGLRSLNVAVAAAMAVGEALRQTGSAIQ 152
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A537VQM6_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A537VQM6_9PROT)

HSP 1 Score: 221 bits (563), Expect = 1.050e-71
Identity = 109/151 (72.19%), Postives = 121/151 (80.13%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQTGHKLEG 459
            +AL+EPDIPQNTGTILRLAAC G++AHIIEP GF +SDRAFRRAGMDYLDQV L RH SW AF+ WR   G R++LFTT A   YL H F+  DVLLFGRE++GVPE VH AADARLVIPMR GLRSLNVA+ AAMA GEAMRQT     G
Sbjct:    3 IALFEPDIPQNTGTILRLAACLGLEAHIIEPAGFATSDRAFRRAGMDYLDQVALVRHPSWEAFEAWRGGAGARLILFTTRAHHSYLAHAFRRGDVLLFGRETSGVPEKVHAAADARLVIPMREGLRSLNVAMAAAMAVGEAMRQTAAPSPG 153
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A1Q7B813_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Alphaproteobacteria bacterium 13_2_20CM_2_64_7 TaxID=1803507 RepID=A0A1Q7B813_9PROT)

HSP 1 Score: 220 bits (561), Expect = 1.980e-71
Identity = 106/145 (73.10%), Postives = 120/145 (82.76%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQT 441
            +ALYEPDIPQNTGT+LRLAAC G++AHIIEP GFP+SDRAFRRAGMDYLDQV + RH SW AF+ WR     R++LFTT A   YL+H F   DVLLFGRE++GVPE VH AADARLVIPMR GLRSLNVA+ AAMA GEA+RQT
Sbjct:    3 IALYEPDIPQNTGTVLRLAACLGLEAHIIEPAGFPTSDRAFRRAGMDYLDQVEIVRHASWEAFEAWRIGADARLILFTTRARRSYLEHTFGPGDVLLFGRETSGVPEKVHAAADARLVIPMREGLRSLNVAMAAAMAVGEAIRQT 147
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: UPI00185BDC7B (tRNA (Cytidine(34)-2'-O)-methyltransferase n=1 Tax=Rhodoplanes sp. TaxID=1968906 RepID=UPI00185BDC7B)

HSP 1 Score: 220 bits (561), Expect = 2.190e-71
Identity = 106/146 (72.60%), Postives = 121/146 (82.88%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQTG 444
            +ALYEPDIPQNTGTILRLAAC G+ A +IEP GFP++DRAFRRAGMDYLD V L RH S+ AF+ +RRA GLR+VL TT AATPYL+H+F  +DVLL GRES GVPE VH  ADARL+IPMR GLRSLNVA+  AM  GEA+RQTG
Sbjct:    3 IALYEPDIPQNTGTILRLAACLGLDADLIEPAGFPTTDRAFRRAGMDYLDHVRLTRHASFDAFEAFRRARGLRLVLLTTAAATPYLEHRFSADDVLLLGRESGGVPERVHATADARLLIPMRPGLRSLNVAMACAMTVGEALRQTG 148
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BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A537NFX5_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A537NFX5_9PROT)

HSP 1 Score: 221 bits (562), Expect = 5.950e-71
Identity = 102/149 (68.46%), Postives = 127/149 (85.23%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQTGHKL 453
            +AL++PDIPQNTGTILRLAAC G +AHIIEP GFP++DRAFRRAGMDYL+ VT++RH SW AF+ WR +E  R++LFTT A  PYL+++FQ +D+LLFGRES+GVPE VH+AADARL+IPM+ GLRSLNVA+ AAM  GEA+RQT   +
Sbjct:    3 IALFQPDIPQNTGTILRLAACLGTEAHIIEPAGFPTTDRAFRRAGMDYLEHVTIRRHPSWRAFEEWRCSERARLILFTTHATLPYLEYRFQSDDILLFGRESSGVPEEVHKAADARLLIPMQGGLRSLNVAMTAAMGIGEALRQTRRSV 151
[Close]
BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Match: A0A537STU6_9PROT (tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A537STU6_9PROT)

HSP 1 Score: 219 bits (557), Expect = 8.610e-71
Identity = 107/151 (70.86%), Postives = 122/151 (80.79%), Query Frame = 1 
Query:    7 LALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDYLDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLFGRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQTGHKLEG 459
            +ALYEPDIPQNTGTILRLAAC G++AHIIEP GFP+SDRAFRRAGMDYL+QV + RHT W+AF+ WR     R+++FTT A   YL H F+  DVLLFGRES+GVPE VH AADARL IPMR GLRSLNVAV AAMA GEA+RQT  +  G
Sbjct:    3 IALYEPDIPQNTGTILRLAACLGVEAHIIEPAGFPTSDRAFRRAGMDYLNQVRIIRHTGWAAFEAWRGGAAARLLVFTTHAPRSYLAHAFERGDVLLFGRESSGVPEKVHAAADARLRIPMREGLRSLNVAVAAAMAVGEALRQTDARPLG 153
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig17542.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A127ERL3_9HYPH3.220e-7774.83tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
A0A537LDS2_9PROT9.620e-7476.03tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
A0A7C4NBY8_9CHLR1.430e-7373.79Putative tRNA (cytidine(34)-2'-O)-methyltransferas... [more]
A0A537S0Q7_9PROT3.670e-7269.54tRNA (cytidine(34)-2'-O)-methyltransferase n=2 Tax... [more]
A0A1M3C8H1_9BRAD7.640e-7271.33tRNA (cytidine(34)-2'-O)-methyltransferase n=2 Tax... [more]
A0A537VQM6_9PROT1.050e-7172.19tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
A0A1Q7B813_9PROT1.980e-7173.10tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
UPI00185BDC7B2.190e-7172.60tRNA (Cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
A0A537NFX5_9PROT5.950e-7168.46tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]
A0A537STU6_9PROT8.610e-7170.86tRNA (cytidine(34)-2'-O)-methyltransferase n=1 Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig17542contigP-wetherbeei_contig17542:779..1240 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog1245469.S58_55730
Preferred nametrmL
PFAMsSpoU_methylase
Model size462
Max annot lvl28211|Alphaproteobacteria
KEGG koko:K03216
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.21e-71
EggNOG OGsCOG0219@1|root,COG0219@2|Bacteria,1RCY4@1224|Proteobacteria,2U78W@28211|Alphaproteobacteria,3JYG0@41294|Bradyrhizobiaceae
EC2.1.1.207
DescriptionMethylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide
Cds size462
COG categoryJ
BRITEko00000,ko01000,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186119.5233936-CDS-P-wetherbeei_contig17542:778..12401683186119.5233936-CDS-P-wetherbeei_contig17542:778..1240Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig17542 779..1240 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig17542.1.1prot_P-wetherbeei_contig17542.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig17542 779..1240 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig17542.1.1

>prot_P-wetherbeei_contig17542.1.1 ID=prot_P-wetherbeei_contig17542.1.1|Name=mRNA_P-wetherbeei_contig17542.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=154bp
VSLALYEPDIPQNTGTILRLAACFGIQAHIIEPTGFPSSDRAFRRAGMDY
LDQVTLQRHTSWSAFDTWRRAEGLRVVLFTTGAATPYLDHKFQGNDVLLF
GRESAGVPEAVHQAADARLVIPMRAGLRSLNVAVCAAMAAGEAMRQTGHK
LEG*
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mRNA from alignment at P-wetherbeei_contig17542:779..1240-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig17542.1.1 ID=mRNA_P-wetherbeei_contig17542.1.1|Name=mRNA_P-wetherbeei_contig17542.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=462bp|location=Sequence derived from alignment at P-wetherbeei_contig17542:779..1240- (Phaeothamnion wetherbeei SAG_119_79)
GTTTCCCTCGCCCTTTACGAGCCCGATATCCCGCAGAACACCGGCACCAT
CCTGCGGCTGGCGGCCTGCTTCGGCATCCAGGCCCACATCATCGAGCCGA
CCGGATTCCCAAGCTCGGACCGGGCGTTCCGCCGCGCCGGTATGGACTAT
CTCGATCAGGTGACGCTGCAGCGGCATACGTCGTGGAGCGCGTTCGACAC
ATGGCGGCGGGCGGAGGGTTTGCGCGTCGTGTTGTTCACAACCGGGGCTG
CGACGCCCTATCTCGATCATAAGTTTCAGGGGAACGACGTGCTGCTGTTC
GGCCGGGAATCCGCGGGCGTGCCGGAGGCGGTGCATCAGGCGGCCGACGC
GCGGCTGGTCATCCCGATGCGCGCAGGTTTGCGGTCGCTCAACGTCGCGG
TGTGCGCCGCGATGGCGGCGGGCGAAGCGATGCGCCAGACGGGGCACAAG
CTGGAGGGCTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig17542:779..1240-

>mRNA_P-wetherbeei_contig17542.1.1 ID=mRNA_P-wetherbeei_contig17542.1.1|Name=mRNA_P-wetherbeei_contig17542.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=462bp|location=Sequence derived from alignment at P-wetherbeei_contig17542:779..1240- (Phaeothamnion wetherbeei SAG_119_79)
GTTTCCCTCGCCCTTTACGAGCCCGATATCCCGCAGAACACCGGCACCAT
CCTGCGGCTGGCGGCCTGCTTCGGCATCCAGGCCCACATCATCGAGCCGA
CCGGATTCCCAAGCTCGGACCGGGCGTTCCGCCGCGCCGGTATGGACTAT
CTCGATCAGGTGACGCTGCAGCGGCATACGTCGTGGAGCGCGTTCGACAC
ATGGCGGCGGGCGGAGGGTTTGCGCGTCGTGTTGTTCACAACCGGGGCTG
CGACGCCCTATCTCGATCATAAGTTTCAGGGGAACGACGTGCTGCTGTTC
GGCCGGGAATCCGCGGGCGTGCCGGAGGCGGTGCATCAGGCGGCCGACGC
GCGGCTGGTCATCCCGATGCGCGCAGGTTTGCGGTCGCTCAACGTCGCGG
TGTGCGCCGCGATGGCGGCGGGCGAAGCGATGCGCCAGACGGGGCACAAG
CTGGAGGGCTGA
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