mRNA_P-wetherbeei_contig14135.4.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig14135.4.1
Unique NamemRNA_P-wetherbeei_contig14135.4.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: A0A7X9AEK2_9BURK (ATP synthase subunit delta n=1 Tax=Burkholderiales bacterium TaxID=1891238 RepID=A0A7X9AEK2_9BURK)

HSP 1 Score: 137 bits (345), Expect = 9.630e-38
Identity = 73/179 (40.78%), Postives = 116/179 (64.80%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAKKGPDGLDKQLQELIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERLTK 537
            ML+  LA RY++AL+ MA++  +   +Q +EL K+   F  ++ L+ A++SP +   +KK+I++ L +GR+   TL FLY++VDK RELY+ AIL  Y   L ++   VE  V+ A PL   +  Q++  L   TGKKV+++  V P+L+GG VI +GDR+ DGS+R  L +++ERL +
Sbjct:    1 MLDETLAERYSEALYQMAREAGEA-GQQFRELQKIVELFADHRELRHALKSPAISRDVKKNIIRHLAEGRVQPRTLHFLYLVVDKGRELYLPAILRLYERKLHSDEGIVEAKVEVAFPLDSTMEDQIRAHLAELTGKKVQMEVRVRPELIGGAVITVGDRLFDGSVRTHLEKMRERLAQ 178
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: A0A7C6V2R4_9FIRM (ATP synthase subunit delta n=1 Tax=Syntrophomonadaceae bacterium TaxID=2093811 RepID=A0A7C6V2R4_9FIRM)

HSP 1 Score: 121 bits (304), Expect = 1.740e-31
Identity = 71/176 (40.34%), Postives = 111/176 (63.07%), Query Frame = 1 
Query:    4 LNNALAMRYAQALFVMAKKGPDGLDKQLQELIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERL 531
            + NA+A RYA+ALF +AK+    LD    +L ++   F ++  LK  +    + +  KK I++ L +  +  T L+F+ +LVDK RE Y+DAI++ + +LLR ER+     VKTA PL P    +V+Q L  +  K++ELK  V P+L+GGMVI++GDR+IDGS+ ++L  +  RL
Sbjct:    1 MQNAIARRYARALFQLAKE-QGSLDAAASDL-QIISKFLEDPALKDLLGHQRISSRRKKEIVRALWEKLVSRTVLAFIELLVDKHRERYLDAIVEVFSDLLRAERNIAVAEVKTAFPLDPEAEARVRQVLEKHFKKQIELKVSVHPELIGGMVIKVGDRIIDGSVSKRLALMGARL 174
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: UPI00200B4707 (ATP synthase F1 subunit delta n=1 Tax=Fuchsiella alkaliacetigena TaxID=957042 RepID=UPI00200B4707)

HSP 1 Score: 121 bits (303), Expect = 2.530e-31
Identity = 66/183 (36.07%), Postives = 111/183 (60.66%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAKKGPDGLDKQLQELIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERLTKVSAA 549
            MLNN +A +Y+QALF +  +  D L++   +LI V      +  L+  +  P +    KK ++++L    +  T L+FL +L+DK RE +++AILD Y E +  E+  +E+ V +A  LT     ++   L   T K++ LKT+V  DLLGG+V++IGDRV+DGSL+++L  ++E + K+  +
Sbjct:    1 MLNNQIAEKYSQALFELTSE-KDQLNEVKDDLIAVVETISDHNDLQEVIYHPQISNEDKKQLVEKLFADEVSKTLLNFLKLLIDKRREHFLEAILDRYLEYVDREKGILEVEVISAFELTAENKDRLATKLAEVTNKEIRLKTQVDRDLLGGLVLKIGDRVVDGSLKKELESVKEEINKIEVS 182
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: A0A1T4L008_9FIRM (ATP synthase subunit delta n=1 Tax=Selenihalanaerobacter shriftii TaxID=142842 RepID=A0A1T4L008_9FIRM)

HSP 1 Score: 120 bits (302), Expect = 3.570e-31
Identity = 71/187 (37.97%), Postives = 115/187 (61.50%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAKKGPDGLDKQLQE-LIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERLTKVSAATVG 558
            M+NN +A RY+QALF +A +      K+LQ+ LI+V    ++++ L   +  P +    KKS+L  L   +I  T L+FL +L+DK RE  ++AILD Y EL   E   +EI V+TA  L+     ++++ L   T  +V L  ++ P+L+GG+V++IGD+VIDGSL + L  +++ LTK+  + +G
Sbjct:    1 MINNQIAKRYSQALFELAVE--KSKVKELQDDLIEVLDTIEEHEELNEVIYHPRISREDKKSLLDELFGSKISQTLLNFLKLLIDKRREKTLEAILDQYIELANQENKILEIEVETAVELSDDNSVKLKEKLEELTNTEVILDIKINPELIGGLVLKIGDKVIDGSLHKYLQVMKDNLTKIEVSQLG 185
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: T2RMA1_9FIRM (ATP synthase subunit delta n=6 Tax=Dehalobacter TaxID=56112 RepID=T2RMA1_9FIRM)

HSP 1 Score: 120 bits (300), Expect = 7.730e-31
Identity = 69/183 (37.70%), Postives = 107/183 (58.47%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAKKGP--DGLDKQLQELIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERLTKVS 543
            MLN ALA RYAQALF +A +    D +DK+L +L ++     QN  LK  +  P + A  KK +L ++LD  +   T  F+Y+L+D+ R+  +  +   +  L    R+ +E  V +A  LTPA   ++++ +   TGK V L +EV P L+GG  +Q+GDRV+DGS+   L +I++ L K S
Sbjct:    1 MLNGALAHRYAQALFELAVEMSVLDQIDKELHDLAEI---VAQNNELKVLLNHPNIEAETKKKVLAKILDDTVSDMTSHFVYLLIDRRRQNLLSLVQREFARLANEARNLIEAKVTSATALTPAQEEKLKEMIARSTGKNVRLLSEVNPTLIGGAKLQVGDRVMDGSITTALSKIRDELKKTS 180
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: A0A2P6C2Y7_9BACT (ATP synthase subunit delta n=1 Tax=Salinibacter sp. 10B TaxID=1923971 RepID=A0A2P6C2Y7_9BACT)

HSP 1 Score: 120 bits (300), Expect = 7.730e-31
Identity = 71/183 (38.80%), Postives = 105/183 (57.38%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAKKGPDGLDKQLQELIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERLTKVSAA 549
            M  + +A RYA AL+  A++    L+K  ++++ ++   + N  L    +SP +P   K SIL+ LL  R+D   + FL +LV K RE    AILD Y+ L   ++  V+ HV TA PL+  V   V  TL   TGK V L T   PD++GG++I+IGD V DGS+R +L  + +RL + S A
Sbjct:    1 MSQSTVARRYATALYEEAEE-TGALEKVDEDVLMLRESLESNDELARFFESPVIPQEKKDSILQTLLGDRVDQLVVRFLRLLVQKDRETMTKAILDAYQSLRDEQQGIVDAHVTTAHPLSDEVQETVVDTLEEQTGKTVRLHTTEDPDVIGGLIIRIGDHVFDGSVRNKLSNLHDRLRESSLA 182
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: UPI001AE5375B (F0F1 ATP synthase subunit delta n=1 Tax=Symbiobacterium terraclitae TaxID=557451 RepID=UPI001AE5375B)

HSP 1 Score: 119 bits (298), Expect = 1.380e-30
Identity = 65/188 (34.57%), Postives = 112/188 (59.57%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAK-KGPDGLDKQLQELIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERLTKVSAATVGG 561
            MLN A++ RYAQALF +A+ KG   LD+  +EL  V    + +  L+  +    +    K+ +L R+L+G++    L FLYV+V K RE Y+  I   +++L       VE+ V++A PL       ++  L+   GK+V+ +T+V P+L+GG+V+++GD ++DGS+R +L +++ERL    +  + G
Sbjct:    1 MLNQAVSRRYAQALFELAQEKGL--LDQVSRELELVLQMIEADGYLRAFINDAVISPAQKREVLGRVLEGKVSPLVLHFLYVVVQKRRETYLPGIYRAFQDLANEALGVVEVEVRSAVPLAEETARNLEAKLVARLGKRVKFRTQVAPELIGGLVVRVGDTLMDGSVRTRLRRMRERLISSQSKVIEG 186
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: A0A3A6MX34_9FIRM (ATP synthase subunit delta n=1 Tax=Candidatus Desulforudis sp. TaxID=2093368 RepID=A0A3A6MX34_9FIRM)

HSP 1 Score: 119 bits (297), Expect = 1.750e-30
Identity = 63/179 (35.20%), Postives = 111/179 (62.01%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAKKGPD--GLDKQLQELIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERL 531
            M+  A+A RYAQALF +A+   +  G++ +L+ +I+     D+++  +  +  P VPA +KK I+K L  G I  T+ +FL V++++ RE Y+ AI + Y  L    R+  E+ V +A  L       + Q L   TGK++ +K +  P++LGG+V+++GDR++D S++RQL +++ER+
Sbjct:    1 MIKGAVAARYAQALFDIAQDTDNIAGIEGELRGVIQA---LDESRDFQKVIFHPQVPAGVKKEIVKELFAGEISKTSFNFLCVILERHREFYLKAICEAYINLANQTRNIAEVEVTSALELPMVHKVNLMQALSKMTGKEIRVKYQTDPEILGGLVVRLGDRIVDASVKRQLERVKERI 176
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: A0A259USW2_9FIRM (ATP synthase subunit delta n=1 Tax=Sporomusa acidovorans DSM 3132 TaxID=1123286 RepID=A0A259USW2_9FIRM)

HSP 1 Score: 119 bits (297), Expect = 1.850e-30
Identity = 75/188 (39.89%), Postives = 110/188 (58.51%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAKKGP--DGLDKQLQEL-IKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERLTKVSAATV 555
            ML N LA++YAQA++ +AK+    D ++KQL+ +   + G+ D    L T +  P VPAP KK  + R+  G +DG   +FL +L+DK RE  + AI+  Y  L    R+  E+ V TA  LT   L  +   L   TGK + LKT V   L+GG+V++IGD++IDGS+ RQL  ++  L +  AA V
Sbjct:    1 MLTNQLALKYAQAVYEIAKEKAMLDVVEKQLRLVETTIAGHAD----LATLMYHPLVPAPAKKETINRIFTGDLDGFLHNFLLLLIDKRREPALPAIIREYIRLANEARNIAEVEVFTAKELTAEQLEALAAKLSKVTGKNIVLKTTVDQTLIGGVVVKIGDKLIDGSVERQLKMLKAALLESQAAAV 184
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BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Match: A0A3B9M429_9FIRM (ATP synthase subunit delta n=3 Tax=Clostridia TaxID=186801 RepID=A0A3B9M429_9FIRM)

HSP 1 Score: 119 bits (297), Expect = 2.060e-30
Identity = 66/181 (36.46%), Postives = 109/181 (60.22%), Query Frame = 1 
Query:    1 MLNNALAMRYAQALFVMAKKGPDGLDKQLQELIKVKGYFDQNQPLKTAVQSPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRELLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLLGGMVIQIGDRVIDGSLRRQLLQIQERLTKVS 543
            M++ A+A RYA+ LF  A +  D ++K  ++L +V    +Q   L+  ++    P  IKK I++++    +    L+FL +L+DK RE  +  I + Y +LLR  ++ V   VKTA PL P    ++Q+ L   TGK +EL+  V P+L+GG+V+Q+GDRV DGS+ ++L  ++ER  + S
Sbjct:    1 MIHQAVARRYAKGLFDTAWE-MDLIEKAAEDLDQVVDLIEQQPDLRKVLEYGGTPTKIKKEIIRKVFAESLSPVVLAFLELLIDKHRERNLYDIRESYHDLLRKHKNTVVASVKTAYPLEPQFEAKLQEVLKKATGKNIELQVSVHPELIGGLVVQVGDRVFDGSITKRLEMMKERFMERS 180
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig14135.4.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7X9AEK2_9BURK9.630e-3840.78ATP synthase subunit delta n=1 Tax=Burkholderiales... [more]
A0A7C6V2R4_9FIRM1.740e-3140.34ATP synthase subunit delta n=1 Tax=Syntrophomonada... [more]
UPI00200B47072.530e-3136.07ATP synthase F1 subunit delta n=1 Tax=Fuchsiella a... [more]
A0A1T4L008_9FIRM3.570e-3137.97ATP synthase subunit delta n=1 Tax=Selenihalanaero... [more]
T2RMA1_9FIRM7.730e-3137.70ATP synthase subunit delta n=6 Tax=Dehalobacter Ta... [more]
A0A2P6C2Y7_9BACT7.730e-3138.80ATP synthase subunit delta n=1 Tax=Salinibacter sp... [more]
UPI001AE5375B1.380e-3034.57F0F1 ATP synthase subunit delta n=1 Tax=Symbiobact... [more]
A0A3A6MX34_9FIRM1.750e-3035.20ATP synthase subunit delta n=1 Tax=Candidatus Desu... [more]
A0A259USW2_9FIRM1.850e-3039.89ATP synthase subunit delta n=1 Tax=Sporomusa acido... [more]
A0A3B9M429_9FIRM2.060e-3036.46ATP synthase subunit delta n=3 Tax=Clostridia TaxI... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig14135contigP-wetherbeei_contig14135:1149..1712 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog1131462.DCF50_p2881
Preferred nameatpH
PFAMsOSCP
Model size564
Max annot lvl186801|Clostridia
KEGG koko:K02113
KEGG TC3.A.2.1
KEGG Pathwayko00190,ko00195,ko01100,map00190,map00195,map01100
KEGG ModuleM00157
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.27e-31
EggNOG OGsCOG0712@1|root,COG0712@2|Bacteria,1VAG3@1239|Firmicutes,24MSA@186801|Clostridia,261U6@186807|Peptococcaceae
DescriptionF(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
Cds size564
COG categoryC
BRITEko00000,ko00001,ko00002,ko00194
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186010.2979927-CDS-P-wetherbeei_contig14135:1148..17121683186010.2979927-CDS-P-wetherbeei_contig14135:1148..1712Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig14135 1149..1712 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig14135.4.1prot_P-wetherbeei_contig14135.4.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig14135 1149..1712 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig14135.4.1

>prot_P-wetherbeei_contig14135.4.1 ID=prot_P-wetherbeei_contig14135.4.1|Name=mRNA_P-wetherbeei_contig14135.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=188bp
MLNNALAMRYAQALFVMAKKGPDGLDKQLQELIKVKGYFDQNQPLKTAVQ
SPTVPAPIKKSILKRLLDGRIDGTTLSFLYVLVDKSRELYVDAILDGYRE
LLRNERSEVEIHVKTAAPLTPAVLGQVQQTLLGYTGKKVELKTEVVPDLL
GGMVIQIGDRVIDGSLRRQLLQIQERLTKVSAATVGG*
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mRNA from alignment at P-wetherbeei_contig14135:1149..1712+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig14135.4.1 ID=mRNA_P-wetherbeei_contig14135.4.1|Name=mRNA_P-wetherbeei_contig14135.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=564bp|location=Sequence derived from alignment at P-wetherbeei_contig14135:1149..1712+ (Phaeothamnion wetherbeei SAG_119_79)
ATGCTAAATAACGCTCTGGCCATGCGATACGCGCAAGCGCTCTTCGTGAT
GGCGAAAAAGGGTCCCGATGGTTTGGATAAACAGCTTCAGGAGCTGATTA
AAGTCAAAGGTTACTTCGATCAAAATCAACCCCTGAAAACGGCTGTCCAG
TCTCCCACCGTGCCGGCTCCCATCAAGAAGTCAATCCTCAAGCGTCTGCT
CGACGGGCGCATCGACGGAACGACTCTCAGCTTCCTCTACGTGCTTGTCG
ACAAGAGCCGCGAGCTCTACGTCGACGCCATCCTAGATGGCTACCGTGAG
CTACTTCGCAACGAACGTAGCGAAGTGGAAATTCACGTAAAGACCGCCGC
GCCGCTCACGCCCGCGGTGCTAGGCCAAGTTCAGCAGACCCTGCTCGGTT
ATACCGGCAAAAAGGTCGAGCTGAAGACCGAGGTCGTTCCCGACCTGCTT
GGCGGAATGGTCATTCAAATCGGGGATCGGGTTATCGACGGCAGCTTGCG
TCGTCAACTGTTGCAGATTCAGGAGCGGCTGACCAAGGTTAGTGCCGCCA
CAGTGGGAGGTTAA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig14135:1149..1712+

>mRNA_P-wetherbeei_contig14135.4.1 ID=mRNA_P-wetherbeei_contig14135.4.1|Name=mRNA_P-wetherbeei_contig14135.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=564bp|location=Sequence derived from alignment at P-wetherbeei_contig14135:1149..1712+ (Phaeothamnion wetherbeei SAG_119_79)
ATGCTAAATAACGCTCTGGCCATGCGATACGCGCAAGCGCTCTTCGTGAT
GGCGAAAAAGGGTCCCGATGGTTTGGATAAACAGCTTCAGGAGCTGATTA
AAGTCAAAGGTTACTTCGATCAAAATCAACCCCTGAAAACGGCTGTCCAG
TCTCCCACCGTGCCGGCTCCCATCAAGAAGTCAATCCTCAAGCGTCTGCT
CGACGGGCGCATCGACGGAACGACTCTCAGCTTCCTCTACGTGCTTGTCG
ACAAGAGCCGCGAGCTCTACGTCGACGCCATCCTAGATGGCTACCGTGAG
CTACTTCGCAACGAACGTAGCGAAGTGGAAATTCACGTAAAGACCGCCGC
GCCGCTCACGCCCGCGGTGCTAGGCCAAGTTCAGCAGACCCTGCTCGGTT
ATACCGGCAAAAAGGTCGAGCTGAAGACCGAGGTCGTTCCCGACCTGCTT
GGCGGAATGGTCATTCAAATCGGGGATCGGGTTATCGACGGCAGCTTGCG
TCGTCAACTGTTGCAGATTCAGGAGCGGCTGACCAAGGTTAGTGCCGCCA
CAGTGGGAGGTTAA
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