mRNA_P-wetherbeei_contig13195.3.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig13195.3.1
Unique NamemRNA_P-wetherbeei_contig13195.3.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: A0A7X9Q3F8_9ACTN (Sigma-70 family RNA polymerase sigma factor n=2 Tax=Actinobacteria TaxID=201174 RepID=A0A7X9Q3F8_9ACTN)

HSP 1 Score: 201 bits (510), Expect = 3.240e-63
Identity = 102/157 (64.97%), Postives = 127/157 (80.89%), Query Frame = 1
Query:    1 LVATRAAFADVYRLEYPGLVAVATALAGD-AEAEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGPSPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENEL 468
            ++AT  +F ++YR EYP LVAVATALAG  A+ ED+VQDTM+KAL+ W  V  L+RPGAWCH VL+NACRS  RR   + RFL+R RR+EP+T GP+ +V+AFW AVRRLPERPR+ +AL+YAGD +V +VA+IL VPEGTVRSDL+RAR AL NEL
Sbjct:   22 IIATEVSFEELYRREYPALVAVATALAGTPADGEDVVQDTMVKALMHWGKVRRLERPGAWCHRVLLNACRSRWRRRRTEARFLARQRREEPSTQGPTVEVVAFWAAVRRLPERPRLAVALYYAGDRSVAEVASILDVPEGTVRSDLSRARMALTNEL 178          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: A0A6N2DQF2_9ACTN (SigE family RNA polymerase sigma factor n=1 Tax=Ilumatobacter sp. TaxID=1967498 RepID=A0A6N2DQF2_9ACTN)

HSP 1 Score: 119 bits (299), Expect = 1.070e-30
Identity = 69/152 (45.39%), Postives = 93/152 (61.18%), Query Frame = 1
Query:   22 FADVYRLEYPGLVAVATALAGDAEA-EDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGP-SPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENELR 471
            F + +  E+P +VA+A+ L G +E   D+ QD+M+KA   W  V +LDRPGAW   V +NA  S HRR + +R   SRLR   P    P  P   +FW AVR LP R R  + LH+  DL+V +VA ILGV EGTV++ L + R+ LE +LR
Sbjct:   71 FDEFFTDEFPKVVAIASVLTGASEVGRDVAQDSMVKAFRDWSKVSELDRPGAWVRRVTINAAVSWHRRNSRERAARSRLR---PGVAQPVQPPEYSFWEAVRGLPSRQRAAVVLHHLEDLSVAEVAEILGVAEGTVKASLAKGRARLEPQLR 219          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: UPI001BBCD9DC (Sigma-70 family RNA polymerase sigma factor n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=UPI001BBCD9DC)

HSP 1 Score: 117 bits (293), Expect = 2.120e-30
Identity = 70/147 (47.62%), Postives = 86/147 (58.50%), Query Frame = 1
Query:   22 FADVYRLEYPGLVAVATALAG-DAEAEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRL--RRQEPATGGP--SPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRAR 447
            FA  Y   +   +AV  AL G D  AE++VQ+  L A  RW+ V  +DRP  W H V +NA  S  RR+  + R L+RL  RR   ATG P  + D + FW  VRRLP   R  +ALHYA DL V DVA +LG PEGTV+S L  AR
Sbjct:   18 FATFYARHHASTLAVVLALRGSDVPAEEIVQEAFLVAYRRWDDVAGMDRPDLWVHRVALNAATSRLRRLAAETRALTRLAGRRDAEATGVPPLADDDVRFWATVRRLPRAQREAVALHYAADLPVADVATVLGCPEGTVKSRLHAAR 164          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: A0A7W1R6X8_9ACTN (Sigma-70 family RNA polymerase sigma factor n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A7W1R6X8_9ACTN)

HSP 1 Score: 115 bits (288), Expect = 9.880e-30
Identity = 66/156 (42.31%), Postives = 90/156 (57.69%), Query Frame = 1
Query:    4 VATRAAFADVYRLEYPGLVAVATALAGDAE-AEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGPSPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENEL 468
            +A   ++   +R E+P +VA+   L G+ E A DL Q+ ML+   RW+ VG  DRP  W   VL+N     HRR   +R   SRL R +       PD   +W AVR LP+R R  +ALHY  D ++ DVA ILG+ EGTV+S L +AR  LE++L
Sbjct:    6 IAVTQSYEAFFREEFPKMVALGLGLTGERETARDLAQEAMLRGYQRWDTVGRFDRPACWVRRVLINLATDHHRRRRTERTNESRLVRDDRVVLD-DPDDERWWDAVRALPDRQRAAVALHYLEDRSIADVAEILGIAEGTVKSTLAKARRKLEHDL 160          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: A0A7G9RGF8_9ACTN (Sigma-70 family RNA polymerase sigma factor n=1 Tax=Nocardioides mesophilus TaxID=433659 RepID=A0A7G9RGF8_9ACTN)

HSP 1 Score: 114 bits (285), Expect = 5.070e-29
Identity = 66/149 (44.30%), Postives = 88/149 (59.06%), Query Frame = 1
Query:   22 FADVYRLEYPGLVAVATALAGDAEAEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGPSPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENEL 468
            F   YR EYP L+ +A  +AG A A+D+VQ+TML A  +WE V     P  W   V +    ++ RR +V+RR L  LR Q  A      D  AFW  VR LP+R   V+ALHYA DL V ++A+ LG  EGTV++ L+RAR+ L  +L
Sbjct:   34 FESFYRREYPHLLVLARVMAGPAVADDVVQETMLTAYHQWEDVQSYASPVGWLRTVCLRKAVNVVRRRSVERRVLGVLRNQPAAPVADVGDGEAFWSLVRTLPQRQAQVVALHYALDLGVAEIASTLGCAEGTVKAHLSRARAGLAEKL 182          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: UPI001B86DBB6 (SigE family RNA polymerase sigma factor n=2 Tax=Stackebrandtia TaxID=283810 RepID=UPI001B86DBB6)

HSP 1 Score: 113 bits (282), Expect = 9.330e-29
Identity = 69/150 (46.00%), Postives = 85/150 (56.67%), Query Frame = 1
Query:   22 FADVYRLEYPGLVAVATALAGDA-EAEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGPSPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENEL 468
            F  +Y   +  L A   A   DA EA+DLVQ+  L+A  RW+ VG  D P AW   V  N   S HRR+ V RRFL R    EP  G  SPD +A   A+R+LPE+ R  + LHY GDL+V ++AA  G  EGTV+S L R RS L   L
Sbjct:    7 FIALYETRFGELAAQVYAYTSDASEAQDLVQEAFLRAWQRWDTVGGYDEPLAWIRRVAWNLATSRHRRVAVARRFLQRSSPPEPHPGM-SPDHVALVAALRKLPEKQRRAVVLHYLGDLSVAEIAAQTGAKEGTVKSWLHRGRSELARYL 155          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: A0A562UY48_9ACTN (RNA polymerase sigma factor n=1 Tax=Stackebrandtia albiflava TaxID=406432 RepID=A0A562UY48_9ACTN)

HSP 1 Score: 113 bits (282), Expect = 3.550e-28
Identity = 69/150 (46.00%), Postives = 85/150 (56.67%), Query Frame = 1
Query:   22 FADVYRLEYPGLVAVATALAGDA-EAEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGPSPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENEL 468
            F  +Y   +  L A   A   DA EA+DLVQ+  L+A  RW+ VG  D P AW   V  N   S HRR+ V RRFL R    EP  G  SPD +A   A+R+LPE+ R  + LHY GDL+V ++AA  G  EGTV+S L R RS L   L
Sbjct:   59 FIALYETRFGELAAQVYAYTSDASEAQDLVQEAFLRAWQRWDTVGGYDEPLAWIRRVAWNLATSRHRRVAVARRFLQRSSPPEPHPGM-SPDHVALVAALRKLPEKQRRAVVLHYLGDLSVAEIAAQTGAKEGTVKSWLHRGRSELARYL 207          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: A0A7V8Z633_9ACTN (SigE family RNA polymerase sigma factor n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A7V8Z633_9ACTN)

HSP 1 Score: 111 bits (278), Expect = 4.870e-28
Identity = 65/151 (43.05%), Postives = 85/151 (56.29%), Query Frame = 1
Query:   22 FADVYRLEYPGLVAVATALAGD-AEAEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGP-SPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENEL 468
            F   YR E+P  VA+A AL G  A AED+ Q+TML A   WE +G  D+P AW   V+VN   SLHRR   + R + RL  ++  T     PD    W AVR LP R    +ALHY  +L++ ++AA+LG   GTV++ L R R  L   L
Sbjct:   26 FDAFYRSEFPRAVALAYALTGSGAVAEDMAQETMLAAHRHWERIGAYDQPRAWVRRVVVNRATSLHRRRAAEWRAVRRLASRDTTTVPDLEPDAAEVWAAVRELPRRQAQAIALHYVEELSLEEMAAVLGCSPGTVKTHLHRGRRRLSKRL 176          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: A0A543AV76_9ACTN (RNA polymerase sigma-70 factor (ECF subfamily) n=1 Tax=Stackebrandtia endophytica TaxID=1496996 RepID=A0A543AV76_9ACTN)

HSP 1 Score: 110 bits (275), Expect = 3.940e-27
Identity = 66/150 (44.00%), Postives = 85/150 (56.67%), Query Frame = 1
Query:   22 FADVYRLEYPGLVAVATALAGDA-EAEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGPSPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENEL 468
            F  +Y   +  L A   A   DA EA+DLVQ+  L+A  RW+ +G  D P AW   V  N   S HRR+ V RRFL R    EP  G  SPD +A   A+R++PE+ R  + LHY GDL+V ++AA  G  EGTV+S L R R+ L   L
Sbjct:   59 FIALYESRFAELAAQVFAYTSDASEAQDLVQEAFLRAWQRWDTIGTYDEPLAWVRRVAWNLATSRHRRVAVARRFLQRSTPPEPHPGM-SPDHVALVEALRKVPEKQRRAVVLHYLGDLSVAEIAAQTGAKEGTVKSWLHRGRAELARHL 207          
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Match: A0A7J9Y4C9_9ACTN (SigE family RNA polymerase sigma factor n=1 Tax=Micromonosporaceae bacterium TaxID=1873464 RepID=A0A7J9Y4C9_9ACTN)

HSP 1 Score: 105 bits (263), Expect = 1.150e-25
Identity = 65/156 (41.67%), Postives = 87/156 (55.77%), Query Frame = 1
Query:   10 TRAAFADVYRLEYPGLVAVATALAGDA-EAEDLVQDTMLKALIRWEHVGDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGP--SPDVLAFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARSALENEL 468
            + A F  +Y+  +  L A   A  GDA EA+DLVQ+  ++A  RW  +G+ D P AW   V  N   S HRR+ V RRFL+R     P    P  S D +    A+R++PER R  M LHY  D+++ D+A  LGV EGTV+S L RAR  +   L
Sbjct:    3 SEAEFTALYQARFNDLTAQLYAYIGDATEAQDLVQEAFVRAWQRWHKIGEYDDPVAWVRRVAWNLATSRHRRLAVVRRFLTR---SGPPDHHPAASADHVVLVDALRKIPERQRQAMVLHYLADMSIADIAHDLGVAEGTVKSWLHRARGEMARHL 155          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig13195.3.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7X9Q3F8_9ACTN3.240e-6364.97Sigma-70 family RNA polymerase sigma factor n=2 Ta... [more]
A0A6N2DQF2_9ACTN1.070e-3045.39SigE family RNA polymerase sigma factor n=1 Tax=Il... [more]
UPI001BBCD9DC2.120e-3047.62Sigma-70 family RNA polymerase sigma factor n=1 Ta... [more]
A0A7W1R6X8_9ACTN9.880e-3042.31Sigma-70 family RNA polymerase sigma factor n=1 Ta... [more]
A0A7G9RGF8_9ACTN5.070e-2944.30Sigma-70 family RNA polymerase sigma factor n=1 Ta... [more]
UPI001B86DBB69.330e-2946.00SigE family RNA polymerase sigma factor n=2 Tax=St... [more]
A0A562UY48_9ACTN3.550e-2846.00RNA polymerase sigma factor n=1 Tax=Stackebrandtia... [more]
A0A7V8Z633_9ACTN4.870e-2843.05SigE family RNA polymerase sigma factor n=1 Tax=Ac... [more]
A0A543AV76_9ACTN3.940e-2744.00RNA polymerase sigma-70 factor (ECF subfamily) n=1... [more]
A0A7J9Y4C9_9ACTN1.150e-2541.67SigE family RNA polymerase sigma factor n=1 Tax=Mi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig13195contigP-wetherbeei_contig13195:762..1235 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog1122182.KB903818_gene1974
PFAMsSigma70_r2,Sigma70_r4_2
Model size474
Max annot lvl201174|Actinobacteria
Hectar predicted targeting categoryother localisation
Exons1
Evalue3.5e-29
EggNOG OGsCOG1595@1|root,COG1595@2|Bacteria,2HUC6@201174|Actinobacteria,4DIYP@85008|Micromonosporales
DescriptionSigma-70, region 4
Cds size474
COG categoryK
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185976.8462029-CDS-P-wetherbeei_contig13195:761..12351683185976.8462029-CDS-P-wetherbeei_contig13195:761..1235Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig13195 762..1235 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig13195.3.1prot_P-wetherbeei_contig13195.3.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig13195 762..1235 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig13195.3.1

>prot_P-wetherbeei_contig13195.3.1 ID=prot_P-wetherbeei_contig13195.3.1|Name=mRNA_P-wetherbeei_contig13195.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=158bp
LVATRAAFADVYRLEYPGLVAVATALAGDAEAEDLVQDTMLKALIRWEHV
GDLDRPGAWCHHVLVNACRSLHRRITVQRRFLSRLRRQEPATGGPSPDVL
AFWIAVRRLPERPRMVMALHYAGDLTVPDVAAILGVPEGTVRSDLTRARS
ALENELR*
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mRNA from alignment at P-wetherbeei_contig13195:762..1235-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig13195.3.1 ID=mRNA_P-wetherbeei_contig13195.3.1|Name=mRNA_P-wetherbeei_contig13195.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=474bp|location=Sequence derived from alignment at P-wetherbeei_contig13195:762..1235- (Phaeothamnion wetherbeei SAG_119_79)
CTCGTCGCGACCCGCGCCGCCTTCGCGGACGTCTACCGCCTCGAGTACCC CGGGCTGGTCGCTGTTGCCACGGCGCTGGCCGGCGACGCGGAAGCAGAGG ACCTCGTCCAGGACACCATGCTGAAGGCGTTGATCCGGTGGGAGCACGTC GGCGACCTCGATCGGCCGGGTGCGTGGTGCCATCACGTCCTCGTCAACGC CTGTCGCAGCCTGCATCGGAGGATCACGGTGCAACGCCGCTTCCTCTCCC GACTTCGTCGACAGGAGCCCGCCACCGGTGGCCCCTCGCCCGACGTCCTC GCGTTCTGGATCGCCGTTCGCCGGCTGCCCGAGCGCCCTCGCATGGTGAT GGCGCTGCACTACGCCGGCGACCTCACCGTGCCCGACGTGGCCGCGATCC TCGGGGTTCCCGAGGGCACGGTCCGTTCCGATCTCACCCGGGCGCGTTCC GCGCTCGAGAACGAACTGAGGTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig13195:762..1235-

>mRNA_P-wetherbeei_contig13195.3.1 ID=mRNA_P-wetherbeei_contig13195.3.1|Name=mRNA_P-wetherbeei_contig13195.3.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=474bp|location=Sequence derived from alignment at P-wetherbeei_contig13195:762..1235- (Phaeothamnion wetherbeei SAG_119_79)
CTCGTCGCGACCCGCGCCGCCTTCGCGGACGTCTACCGCCTCGAGTACCC
CGGGCTGGTCGCTGTTGCCACGGCGCTGGCCGGCGACGCGGAAGCAGAGG
ACCTCGTCCAGGACACCATGCTGAAGGCGTTGATCCGGTGGGAGCACGTC
GGCGACCTCGATCGGCCGGGTGCGTGGTGCCATCACGTCCTCGTCAACGC
CTGTCGCAGCCTGCATCGGAGGATCACGGTGCAACGCCGCTTCCTCTCCC
GACTTCGTCGACAGGAGCCCGCCACCGGTGGCCCCTCGCCCGACGTCCTC
GCGTTCTGGATCGCCGTTCGCCGGCTGCCCGAGCGCCCTCGCATGGTGAT
GGCGCTGCACTACGCCGGCGACCTCACCGTGCCCGACGTGGCCGCGATCC
TCGGGGTTCCCGAGGGCACGGTCCGTTCCGATCTCACCCGGGCGCGTTCC
GCGCTCGAGAACGAACTGAGGTGA
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