mRNA_P-wetherbeei_contig12169.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig12169.2.1
Unique NamemRNA_P-wetherbeei_contig12169.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: A0A351T4E9_9PROT (SsrA-binding protein n=3 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A351T4E9_9PROT)

HSP 1 Score: 236 bits (602), Expect = 2.930e-77
Identity = 118/167 (70.66%), Postives = 142/167 (85.03%), Query Frame = 1 
Query:    1 MAGKNKAAKKPVKKSDAGRLLADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            MAGKN+  K   K    GRLLA+NRKARYNY+I ETVEAG+ L GSEVKSLR GRANIAESYAS EG+E+WLINAYIPPY  AGRY++H+PRRKRKLLLHR+QI +L+GLR+++G+TLIPLRLYFNDRG+AKL + LA GKK HDKR +EK RDW R++ RL+R++G
Sbjct:    1 MAGKNRGKK--AKGDATGRLLAENRKARYNYQIEETVEAGVALTGSEVKSLREGRANIAESYASEEGNEIWLINAYIPPYSHAGRYNNHEPRRKRKLLLHRRQIGKLAGLRQKEGMTLIPLRLYFNDRGMAKLALGLARGKKMHDKRETEKKRDWDRQKARLLRDRG 165
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: A0A6N0DYJ0_9HYPH (SsrA-binding protein n=1 Tax=Methyloligella sp. GL2 TaxID=2742204 RepID=A0A6N0DYJ0_9HYPH)

HSP 1 Score: 203 bits (516), Expect = 2.670e-64
Identity = 101/154 (65.58%), Postives = 129/154 (83.77%), Query Frame = 1 
Query:   40 KSDAGRLLADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            K   G+ +A+NRKAR+NYEIG+ +EAGI+L GSEVKSLR G+ANIAESYASAE  EM+LINA+IP Y  AGR D+HDPRR+RKLLLH+K++ RLSG  +++GIT+IPLRLYFNDRG+AK+ + +  GKK HDKR +EK RDW R++ R+MRE+G
Sbjct:    4 KHGTGKTIAENRKARFNYEIGDKLEAGIVLAGSEVKSLRTGKANIAESYASAEDGEMYLINAHIPEYVQAGR-DNHDPRRRRKLLLHKKEMDRLSGAIQREGITVIPLRLYFNDRGVAKVELGVGKGKKLHDKRETEKKRDWDRQKARIMRERG 156
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: A0A3D3UKG4_9PROT (SsrA-binding protein (Fragment) n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A3D3UKG4_9PROT)

HSP 1 Score: 199 bits (505), Expect = 5.580e-63
Identity = 96/131 (73.28%), Postives = 117/131 (89.31%), Query Frame = 1 
Query:  109 VEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            +EAGIML GSEVKSLR GRANIAESYAS EG+E+WLINAYIPPY  AGRY++H+PRRKRKLLLHR+QI +L+GLR+++G+TLIPLRLYFNDRG+AKL + LA GKK HDKR +EK RDW R++ RL+R++G
Sbjct:    1 LEAGIMLVGSEVKSLREGRANIAESYASEEGNEIWLINAYIPPYSHAGRYNNHEPRRKRKLLLHRRQIGKLAGLRQKEGMTLIPLRLYFNDRGMAKLALGLARGKKMHDKRETEKKRDWDRQKARLLRDRG 131
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: UPI001FA6B892 (SsrA-binding protein SmpB n=2 Tax=unclassified Caulobacter TaxID=2648921 RepID=UPI001FA6B892)

HSP 1 Score: 198 bits (504), Expect = 1.850e-62
Identity = 104/161 (64.60%), Postives = 132/161 (81.99%), Query Frame = 1 
Query:   19 AAKKPVKKSDAGRLLADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            A KKP+    A +L+A+NR+AR++Y + +T EAG+ L G+EVK+LR GRANIAESYAS EGDE+ LINAYIP Y PA R++ H+PRR RKLLLHR++I+RL G  +++G TLIP RLYFN++G AKL ++LA GKK HDKRA+E DRDWKREQGRLMR+KG
Sbjct:    2 AEKKPL----AQKLVAENRRARFDYFLEQTFEAGLSLTGTEVKALREGRANIAESYASTEGDEIVLINAYIPEYGPANRFN-HEPRRPRKLLLHRREINRLLGSIQREGRTLIPTRLYFNEKGRAKLELALAKGKKLHDKRATEADRDWKREQGRLMRDKG 157
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: A0A5J4DYW1_9BACT (SsrA-binding protein n=1 Tax=bacterium MnTg02 TaxID=2080452 RepID=A0A5J4DYW1_9BACT)

HSP 1 Score: 197 bits (501), Expect = 5.110e-62
Identity = 95/154 (61.69%), Postives = 131/154 (85.06%), Query Frame = 1 
Query:   40 KSDAGRLLADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            K D  +++ADNR+ARYNY+I ET EAGIML+GSEVKSLRAG+ANIAE+YAS E  E++L+N+ IP Y  AGR++ H+PRR+RKLLLH+++I +L G  +++G+T++PL+LYFNDRG+AKL +++A GKK HDKR +EK RDW+R++ R+MREKG
Sbjct:    4 KKDGRKIVADNRRARYNYQIEETFEAGIMLQGSEVKSLRAGKANIAEAYASEEDGEIYLLNSNIPTYKQAGRFN-HEPRRQRKLLLHKREIDKLLGAVQREGMTIVPLKLYFNDRGIAKLELAIARGKKLHDKRETEKKRDWQRQKARIMREKG 156
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: UPI00096B88F0 (SsrA-binding protein SmpB n=1 Tax=Salaquimonas pukyongi TaxID=2712698 RepID=UPI00096B88F0)

HSP 1 Score: 196 bits (497), Expect = 2.290e-61
Identity = 100/158 (63.29%), Postives = 128/158 (81.01%), Query Frame = 1 
Query:   28 KPVKKSDAGRLLADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            K  K S  G+L+ADNRKAR+NYE  ET+EAG++L G+EVKSLR G+ANIAESYA+ EG E+WLIN+YIP Y    R++ H+PRRKRKLLL++++++RLS    + G+T++P RLYFN+RG AKL I+LA G+K HDKR   KDRDWKREQGRL+REKG
Sbjct:    3 KKKKDSANGKLIADNRKARHNYEFVETLEAGLVLTGTEVKSLRNGKANIAESYATEEGGEIWLINSYIPEYLEGNRHN-HEPRRKRKLLLNKREMARLSQAVARDGMTIVPNRLYFNERGRAKLQIALAKGRKVHDKRQVSKDRDWKREQGRLLREKG 159
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: A0A1Q3JB86_9PROT (SsrA-binding protein n=1 Tax=Caulobacterales bacterium 68-7 TaxID=1895737 RepID=A0A1Q3JB86_9PROT)

HSP 1 Score: 195 bits (495), Expect = 3.440e-61
Identity = 100/150 (66.67%), Postives = 123/150 (82.00%), Query Frame = 1 
Query:   52 GRLLADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            G+L+A+NR+ARY+Y + + VE GI L G+EVKSLR GRANIAESYA+ EGDE+ LINA IPPY    R++ H+PRR RKLLLHRKQI +LS   ++ G TLIP +LY+ND+GLAKL I++A GKK HDKRA+E DRDWKREQGRLMR+KG
Sbjct:    2 GKLIAENRRARYDYFLDDAVECGIALTGTEVKSLRTGRANIAESYAAVEGDEIVLINADIPPYAGGNRFN-HEPRRPRKLLLHRKQIDKLSAAVQRDGQTLIPTKLYWNDKGLAKLEIAVAKGKKLHDKRATEADRDWKREQGRLMRDKG 150
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: UPI00136A1EF0 (SsrA-binding protein SmpB n=1 Tax=Propylenella binzhouense TaxID=2555902 RepID=UPI00136A1EF0)

HSP 1 Score: 195 bits (495), Expect = 4.320e-61
Identity = 95/148 (64.19%), Postives = 122/148 (82.43%), Query Frame = 1 
Query:   58 LLADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            ++ADNR+AR+NYEIGET EAGI L G+EVKSLR  +ANIAESY S EG E+WLIN+YIP Y  A  ++ H+PRR RKLLLH+++++RL G   ++G+T++PLR+YFNDRG AK+ I+LA GKK HDKR +EK RDW RE+GRL+REKG
Sbjct:   11 VVADNRRARFNYEIGETFEAGIALAGTEVKSLRQSKANIAESYVSPEGGEVWLINSYIPEYQQASFFN-HEPRRPRKLLLHKREVNRLFGAVNREGMTIVPLRMYFNDRGRAKVEIALAKGKKVHDKRETEKQRDWAREKGRLLREKG 157
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: I3TKY6_TISMK (SsrA-binding protein n=6 Tax=Tistrella TaxID=171436 RepID=I3TKY6_TISMK)

HSP 1 Score: 194 bits (494), Expect = 6.330e-61
Identity = 97/147 (65.99%), Postives = 123/147 (83.67%), Query Frame = 1 
Query:   61 LADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            +A NRKAR++Y I +TVEAG++L G+EVKSLR G+ NIAESYAS E +E WLINA IP Y    R++ H+PRRKRKLLLHRK+I+RL GL K++G+T++PL LYFND+G+AKL + LA GKK HDKRA+E+DRDW R++GRLMREKG
Sbjct:   13 VAVNRKARFDYFIEDTVEAGLVLTGTEVKSLRGGKCNIAESYASVESNEAWLINANIPEYGGGNRFN-HEPRRKRKLLLHRKEINRLFGLIKREGVTIVPLVLYFNDKGMAKLKLGLAKGKKLHDKRATERDRDWDRQKGRLMREKG 158
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BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Match: J6J411_9RHOB (SsrA-binding protein n=1 Tax=Rhodovulum sp. PH10 TaxID=1187851 RepID=J6J411_9RHOB)

HSP 1 Score: 194 bits (494), Expect = 6.330e-61
Identity = 96/155 (61.94%), Postives = 125/155 (80.65%), Query Frame = 1 
Query:   37 KKSDAGRLLADNRKARYNYEIGETVEAGIMLKGSEVKSLRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLHRKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASEKDRDWKREQGRLMREKG 501
            KK    +++ADNRKAR+NYEIGE VEAGI L GSEVKSLR GRA I ESYA A+G E+WL+NA IP Y  AG + +H+P+R+RKLLLH++QI++L+   +++G+T++PLR+YFN+RG AKL I+L  GKK HDKR +EK RDW RE+GRL+R KG
Sbjct:    4 KKEPGRKIVADNRKARFNYEIGEVVEAGISLLGSEVKSLRQGRATIGESYADAKGGEIWLVNANIPEYLQAGPFFNHEPKRRRKLLLHKRQIAKLAQGIEREGMTIVPLRMYFNERGKAKLEIALGRGKKLHDKRETEKKRDWNREKGRLLRAKG 158
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig12169.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A351T4E9_9PROT2.930e-7770.66SsrA-binding protein n=3 Tax=Alphaproteobacteria b... [more]
A0A6N0DYJ0_9HYPH2.670e-6465.58SsrA-binding protein n=1 Tax=Methyloligella sp. GL... [more]
A0A3D3UKG4_9PROT5.580e-6373.28SsrA-binding protein (Fragment) n=1 Tax=Alphaprote... [more]
UPI001FA6B8921.850e-6264.60SsrA-binding protein SmpB n=2 Tax=unclassified Cau... [more]
A0A5J4DYW1_9BACT5.110e-6261.69SsrA-binding protein n=1 Tax=bacterium MnTg02 TaxI... [more]
UPI00096B88F02.290e-6163.29SsrA-binding protein SmpB n=1 Tax=Salaquimonas puk... [more]
A0A1Q3JB86_9PROT3.440e-6166.67SsrA-binding protein n=1 Tax=Caulobacterales bacte... [more]
UPI00136A1EF04.320e-6164.19SsrA-binding protein SmpB n=1 Tax=Propylenella bin... [more]
I3TKY6_TISMK6.330e-6165.99SsrA-binding protein n=6 Tax=Tistrella TaxID=17143... [more]
J6J411_9RHOB6.330e-6161.94SsrA-binding protein n=1 Tax=Rhodovulum sp. PH10 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig12169contigP-wetherbeei_contig12169:559..1062 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog1110502.TMO_1585
Preferred namesmpB
PFAMsSmpB
Model size504
Max annot lvl204441|Rhodospirillales
KEGG koko:K03664
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.04e-61
EggNOG OGsCOG0691@1|root,COG0691@2|Bacteria,1RDFP@1224|Proteobacteria,2U71I@28211|Alphaproteobacteria,2JSVE@204441|Rhodospirillales
Descriptionthe 2 termini fold to resemble tRNA(Ala) and it encodes a tag peptide , a short internal open reading frame. During trans-translation Ala- aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA
Cds size504
COG categoryO
BRITEko00000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185939.9716196-CDS-P-wetherbeei_contig12169:558..10621683185939.9716196-CDS-P-wetherbeei_contig12169:558..1062Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig12169 559..1062 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig12169.2.1prot_P-wetherbeei_contig12169.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig12169 559..1062 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig12169.2.1

>prot_P-wetherbeei_contig12169.2.1 ID=prot_P-wetherbeei_contig12169.2.1|Name=mRNA_P-wetherbeei_contig12169.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=168bp
MAGKNKAAKKPVKKSDAGRLLADNRKARYNYEIGETVEAGIMLKGSEVKS
LRAGRANIAESYASAEGDEMWLINAYIPPYDPAGRYDSHDPRRKRKLLLH
RKQISRLSGLRKQQGITLIPLRLYFNDRGLAKLVISLASGKKQHDKRASE
KDRDWKREQGRLMREKG*
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mRNA from alignment at P-wetherbeei_contig12169:559..1062-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig12169.2.1 ID=mRNA_P-wetherbeei_contig12169.2.1|Name=mRNA_P-wetherbeei_contig12169.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=504bp|location=Sequence derived from alignment at P-wetherbeei_contig12169:559..1062- (Phaeothamnion wetherbeei SAG_119_79)
ATGGCAGGCAAAAACAAAGCCGCGAAGAAGCCGGTTAAAAAATCAGATGC
AGGGCGGTTGCTGGCTGATAATCGCAAGGCGCGCTATAATTATGAAATTG
GCGAAACGGTGGAAGCCGGGATCATGCTCAAGGGATCGGAGGTGAAGTCG
TTGCGCGCGGGCCGCGCCAATATTGCGGAATCCTATGCCAGCGCAGAAGG
CGATGAGATGTGGCTGATCAATGCCTATATCCCGCCCTATGATCCCGCCG
GCCGGTATGACAGCCACGATCCGCGCCGCAAACGCAAGCTGTTATTGCAT
CGCAAGCAGATCAGCCGCCTGTCAGGGTTGCGCAAACAGCAGGGCATTAC
GCTGATCCCGTTGCGGCTGTACTTCAATGATCGTGGTCTGGCCAAGCTGG
TCATTTCTCTGGCCAGCGGCAAAAAACAGCATGACAAGCGCGCCAGTGAA
AAAGACCGCGACTGGAAACGCGAACAGGGCCGCCTGATGCGCGAGAAAGG
ATAG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig12169:559..1062-

>mRNA_P-wetherbeei_contig12169.2.1 ID=mRNA_P-wetherbeei_contig12169.2.1|Name=mRNA_P-wetherbeei_contig12169.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=504bp|location=Sequence derived from alignment at P-wetherbeei_contig12169:559..1062- (Phaeothamnion wetherbeei SAG_119_79)
ATGGCAGGCAAAAACAAAGCCGCGAAGAAGCCGGTTAAAAAATCAGATGC
AGGGCGGTTGCTGGCTGATAATCGCAAGGCGCGCTATAATTATGAAATTG
GCGAAACGGTGGAAGCCGGGATCATGCTCAAGGGATCGGAGGTGAAGTCG
TTGCGCGCGGGCCGCGCCAATATTGCGGAATCCTATGCCAGCGCAGAAGG
CGATGAGATGTGGCTGATCAATGCCTATATCCCGCCCTATGATCCCGCCG
GCCGGTATGACAGCCACGATCCGCGCCGCAAACGCAAGCTGTTATTGCAT
CGCAAGCAGATCAGCCGCCTGTCAGGGTTGCGCAAACAGCAGGGCATTAC
GCTGATCCCGTTGCGGCTGTACTTCAATGATCGTGGTCTGGCCAAGCTGG
TCATTTCTCTGGCCAGCGGCAAAAAACAGCATGACAAGCGCGCCAGTGAA
AAAGACCGCGACTGGAAACGCGAACAGGGCCGCCTGATGCGCGAGAAAGG
ATAG
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